FastQCFastQC Report
Thu 4 May 2017
HCGCLBCXY_s1_1_illumina12index_8_SL248372.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s1_1_illumina12index_8_SL248372.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 8641096
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 9960160 8.261842 56.750416 110-119
ATACA 9808455 7.833671 39.05314 110-119
CACAG 12969365 7.316342 42.690357 110-119
TCCCT 15973675 7.214395 117.03591 30-34
CAGTA 12018350 7.0415125 45.255768 60-69
AGTAA 8831895 6.3369484 45.694607 110-119
CCCTG 16086350 6.284465 78.21907 30-34
GGAGA 13105145 5.966783 93.55694 35-39
GGTGA 15373755 5.936343 102.45006 45-49
GTAAT 9747320 5.9313307 37.99936 110-119
AATAC 7014780 5.6024604 41.231136 110-119
ACAGT 8837465 5.1778426 34.683186 110-119
ACTCC 9584420 5.104112 132.91325 30-34
GTAGT 11253620 5.0236063 22.999563 70-79
CTCAG 10471925 5.0100546 37.154583 140-149
GTGAC 11504290 4.9446697 84.17979 45-49
AGACG 9667140 4.8993087 99.078995 40-44
ACCAG 8461355 4.773261 96.966644 45-49
CCCCA 9156405 4.6949778 99.26545 60-69
CACTC 8722405 4.6450524 108.43581 25-29
GAGAC 9134675 4.6294556 138.4219 40-44
GCACA 7873835 4.4418263 33.849632 110-119
CTCCC 9940155 4.3225737 76.56455 30-34
ATGCA 7322540 4.2902527 104.89607 25-29
GGCCC 12654105 4.276199 61.15925 60-69
TGACC 8667580 4.1468067 141.10454 45-49
CGTGT 11296990 4.1179485 21.408846 120-129
GACCA 7278975 4.106251 108.68956 45-49
GTGTC 11138435 4.0601525 22.617826 120-129
AAAAA 3482195 4.015894 22.746181 130-139
CCCAG 8573430 3.9493415 86.84028 60-69
TAGTA 6483600 3.9453285 26.004953 80-89
AGTAG 7434185 3.9130552 29.528744 60-69
TACAC 5955510 3.8839936 29.276268 110-119
TGCAC 7925790 3.7919142 127.8012 25-29
TGAGG 9659725 3.7299569 69.66644 35-39
AGATA 5197555 3.729283 20.171986 150-159
ATGGT 8337485 3.721846 25.446032 190-199
AGGAG 8157040 3.7139058 94.4903 35-39
CAGGG 9925375 3.6901166 38.954315 50-59
GACGG 9739015 3.6208308 107.92964 40-44
GGATG 9366320 3.6166632 78.14602 20-24
CTGAG 8391345 3.6066923 105.66598 35-39
CCACT 6761070 3.600558 25.557598 240-247
CCTGA 7507075 3.5915897 93.83238 30-34
GGGTT 10964345 3.5905619 45.544968 50-59
ACGGT 8318535 3.5753977 95.29169 40-44
TGTCC 8787825 3.5656443 20.502325 120-129
GCACT 7445755 3.5622525 98.68495 25-29
GAGGA 7773810 3.539421 123.338615 35-39
CAGAT 5958095 3.4908285 18.551962 150-159
CTTCA 6112530 3.3808136 16.023542 200-209
GCCCC 8899505 3.347579 72.82134 60-69
CGGAT 7781965 3.344774 88.16569 20-24
AGGCT 7733610 3.32399 20.740807 140-149
CTCTC 7358915 3.323601 13.771995 100-109
CCAGG 7976505 3.3009906 42.897644 50-59
CCTGG 9370080 3.2886348 30.28041 50-59
TGGTG 9940490 3.2552738 18.034576 190-199
TCAGG 7531450 3.2370996 22.144749 140-149
GATGC 7428780 3.192971 107.02386 25-29
CCCTT 7035555 3.177558 22.125538 20-24
TCTCA 5728190 3.1682367 22.564653 130-139
AGGGT 8189305 3.162176 55.072304 50-59
TCTTG 7435275 3.1332822 17.121408 170-179
GGTTG 9559470 3.1304986 82.08663 15-19
AGTCA 5275390 3.090834 34.01923 70-79
TTCCC 6832520 3.0858586 51.153885 50-59
CCAGT 6371675 3.0483832 42.19727 60-69
CCGTG 8677870 3.0456886 21.718054 120-129
TCGCA 6357630 3.041664 23.19941 100-109
CTTGG 8298650 3.025001 14.412126 170-179
GTTGG 9230430 3.0227456 59.11527 15-19
CGCAC 6524905 3.0056906 23.98811 100-109
TGGCC 8419170 2.9548922 45.11554 60-69
GGTTC 7982195 2.9096484 50.87946 50-59
TGTCT 6796045 2.8639057 18.683931 180-189
ACAGC 5071260 2.860824 12.904861 120-129
GCGGA 7649180 2.843859 99.15066 20-24
TCAAA 3539205 2.8266394 56.81146 70-79
ACCAC 4475030 2.8100219 15.504411 230-239
TCTCT 5967515 2.7992032 14.313736 180-189
CGGTG 8726655 2.7515771 68.81442 40-44
TCCAT 4958210 2.7423642 14.987544 70-79
GCCGT 7704545 2.704079 20.40819 120-129
GCCCT 6894010 2.6932876 16.082268 200-209
CAGCT 5615685 2.686697 13.197199 150-159
GATGG 6945790 2.6820118 13.438521 190-199
AGATG 5092570 2.6805236 17.829758 180-189
GTTCC 6561210 2.6621993 56.975216 50-59
GGCTG 8385110 2.6438854 13.438325 140-149
ACTAC 4037995 2.6334515 14.350032 230-239
TCCTC 5801675 2.6202848 17.574863 130-139
TTCTT 5367585 2.6149642 19.290253 170-179
CGGCC 7734570 2.6137414 11.5256605 120-129
TTGGG 7950425 2.6035748 88.63445 15-19
TACCA 3953395 2.578278 16.917267 230-239
GGCGG 9410245 2.5665545 49.021496 20-24
TGTTC 6072965 2.559194 13.446952 140-149
GAGAT 4731585 2.4905155 17.098984 180-189
TCAGC 5199895 2.4877715 14.944351 140-149
TGAAT 4078405 2.4817457 15.50342 190-199
GACTC 5184390 2.4803538 17.13092 190-199
CACGG 5904130 2.4433606 10.5153265 120-129
TGGGG 8466005 2.3981369 59.746983 15-19
CTGGA 5565500 2.3921127 13.50311 180-189
CTGGC 6814410 2.3916664 36.239307 60-69
GAATC 4044690 2.3697708 15.7677 190-199
GCTGT 6500705 2.3696196 10.512786 140-149
ACAGA 3422205 2.3642123 10.229597 140-149
TCACA 3625020 2.3641224 8.979782 140-149
TCATT 4095995 2.352911 17.262592 150-159
TAGTC 4673300 2.3221245 31.13999 70-79
ACACA 3018395 2.321106 15.685152 120-129
CTACT 4185245 2.3148408 9.517544 230-239
CATTT 4026180 2.3128064 17.41959 150-159
TGGAG 5895720 2.2765431 13.067232 180-189
TTCAT 3960495 2.2750742 17.496563 140-149
TTCAC 4104835 2.2703664 17.51631 200-209
GTTCT 5370125 2.263012 17.424301 160-169
TTGTC 5335165 2.2482798 14.437917 170-179
TACTA 3314320 2.2449145 12.330066 230-239
TACAG 3823985 2.2404602 15.153716 160-169
GATAC 3819365 2.2377536 15.600632 150-159
GTGAA 4248740 2.2363653 17.13267 190-199
ATTTG 4331370 2.235285 15.576744 150-159
CAGGC 5378335 2.225766 17.402637 140-149
ACTCT 4023220 2.2252254 12.99526 190-199
TCTGC 5478830 2.223025 12.033351 210-219
TGGAA 4214445 2.218314 10.486262 170-179
AGGGA 4842400 2.204748 6.8190947 150-159
AGCTC 4607980 2.2045834 13.816181 140-149
ATCTC 3977860 2.2001371 8.549231 100-109
CAGCC 4696135 2.1632698 11.648177 120-129
CTCTG 5283130 2.1436207 14.143852 180-189
AATCG 3654775 2.141321 15.254887 190-199
GGGGC 7837215 2.1375256 55.43133 15-19
AACCA 2775430 2.134269 15.580768 80-89
ACACG 3765775 2.1243675 14.487864 120-129
GTCCT 5230835 2.122402 15.009315 130-139
CTCGC 5387225 2.1046307 16.381975 100-109
AATAA 2149315 2.1021802 9.276521 80-89
CACTA 3215775 2.097226 14.141039 240-247
GGGCG 7645085 2.085124 68.38082 20-24
CGTAG 4801155 2.063589 10.123356 210-219
GTTCA 4149240 2.0617232 15.289092 140-149
GAACT 3474760 2.0358508 8.3623085 160-169
TTGGA 4554225 2.0330021 15.064638 170-179
GATAT 3322020 2.0214787 7.567964 180-189
CTCCA 3783165 2.0146966 9.51343 150-159
CATAG 3425635 2.0070684 8.586791 210-219
TGTAG 4479990 1.9998634 6.765768 150-159
ATATG 3278885 1.995231 7.7928314 180-189
GTCAA 3401840 1.9931271 40.40994 70-79
TTGCA 3988500 1.9818529 14.129404 150-159
TCTCG 4867845 1.9751194 16.37218 100-109
CCTTC 4372915 1.9749956 13.293619 200-209
AGTAT 3228245 1.9644158 13.225074 220-229
TTTGC 4584415 1.9319078 12.334264 150-159
TCGGC 5497505 1.9294698 8.1358595 190-199
AATAT 2281755 1.8926904 10.08818 110-119
CAGAG 3719560 1.8850739 7.3301325 140-149
GTCTC 4638610 1.8821077 11.429169 180-189
ACGGC 4476135 1.8524002 13.471839 120-129
CAAAA 1961180 1.846892 20.982998 70-79
ATATA 2208810 1.8321834 9.899599 240-247
CTGTG 4993655 1.8202736 6.0365496 210-219
ATTGT 3520875 1.8170137 8.504415 170-179
CTGTT 4306435 1.8147649 12.639864 140-149
TGCAG 4203635 1.8067685 13.371771 150-159
GAGTC 4194725 1.8029387 11.688912 210-219
GCAGA 3554785 1.8015659 14.396511 150-159
TGCTC 4338645 1.7603974 6.7401414 150-159
TGTGC 4768820 1.7383174 8.07209 220-229
GTCTG 4745905 1.7299645 9.174217 210-219
ATGTA 2822845 1.7177263 8.244298 150-159
ACCAT 2633580 1.7175368 11.59588 45-49
AGTCT 3456150 1.7173326 12.649254 210-219
TCAGA 2927065 1.714958 10.943022 130-139
ATAGT 2773875 1.6879277 8.227334 220-229
GTGCT 4600040 1.6767943 7.4569855 220-229
CAAAG 2419545 1.6715299 29.444016 70-79
TTCAG 3362305 1.6707017 8.415666 140-149
CACCA 2659700 1.6701151 10.786803 230-239
GCTCC 4195155 1.638924 7.797524 150-159
TCTGG 4414375 1.6091158 11.158103 180-189
GTCAC 3363060 1.6089797 6.6878505 140-149
CCATA 2445430 1.5948313 17.830832 70-79
TGACT 3206390 1.5932287 10.86412 190-199
GCTCT 3912130 1.5873399 10.637715 130-139
AGATC 2682105 1.5714366 8.581802 130-139
ACTTC 2794425 1.5455843 7.7040215 220-229
CTGCT 3808330 1.5452232 6.108216 90-99
CCATC 2882280 1.534937 13.633788 240-247
TAGTT 2974040 1.5348094 10.122036 220-229
CCAGA 2715245 1.5317372 22.381332 60-69
GTTGT 4045915 1.5317248 5.450754 210-219
CCACC 2977965 1.5269617 8.108377 230-239
GAACC 2702490 1.5245419 13.158838 70-79
GCTCA 3161645 1.5126172 10.929102 140-149
ATCGG 3511990 1.5094917 9.130501 190-199
TGGTT 3984175 1.5083511 18.704653 10-14
CCTCA 2832165 1.5082484 9.407671 130-139
TGATA 2443850 1.4871044 6.867061 180-189
GACGT 3451315 1.4834131 7.0086894 170-179
GCGTA 3436985 1.4772537 10.374656 210-219
AATTG 2423415 1.4746698 10.599292 170-179
GGAAT 2801045 1.4743572 9.757276 170-179
TCCCA 2759090 1.4693329 10.347264 240-247
CTGGT 4026415 1.4676981 5.715749 160-169
AACTG 2493125 1.4607139 7.875264 160-169
AGAAC 2087435 1.4420933 8.89873 160-169
GTAGG 3711680 1.4332092 6.39106 160-169
GTCCC 3662865 1.4309739 17.458054 50-59
GAATT 2331440 1.4187019 11.288965 170-179
CTTGA 2843890 1.4131052 6.7422576 200-209
AGCCG 3409560 1.4110097 10.301183 120-129
TTGTA 2706650 1.3968178 6.8236704 210-219
GAGAA 2244795 1.3932166 8.31586 160-169
CCTTG 3423070 1.3889046 14.486242 20-24
GGACG 3735310 1.3887365 5.1699824 170-179
GGTCA 3221045 1.3844403 5.502809 130-139
ATAAC 1733030 1.3841107 9.591489 80-89
ACTGA 2358170 1.3816441 6.6485696 180-189
GTGTT 3644845 1.3798857 8.046646 160-169
GGCCG 4536770 1.3773198 10.916138 120-129
CACTG 2864300 1.3703592 5.186978 230-239
CCTCG 3499390 1.3671088 8.452701 130-139
ACCGT 2838605 1.3580661 15.44527 45-49
AATAG 1889780 1.3559309 8.144104 240-247
ATACC 2078245 1.355365 12.75558 70-79
CCCAC 2632575 1.3498619 9.512504 240-247
ACGGA 2659565 1.3478682 9.731704 200-209
ATCAT 1984275 1.3440247 6.941303 230-239
GATCT 2704620 1.3439034 6.7295184 130-139
TCCAC 2510815 1.337116 9.425641 240-247
TTCCA 2372345 1.3121338 9.330443 230-239
ATAGA 1828060 1.3116462 8.808278 240-247
CTGAT 2628080 1.3058714 6.8151584 180-189
GAGGG 3811555 1.2730834 6.192443 200-209
TTGGT 3340890 1.2648127 15.266463 25-29
AAATA 1290600 1.2622969 5.865466 9
TCTGT 2959565 1.2471833 7.360279 210-219
ATATC 1823345 1.2350209 7.851941 70-79
CAGCG 2980935 1.2336278 7.542952 160-169
CAGAC 2178795 1.2291124 12.696317 60-69
CTCTT 2618340 1.2281941 6.79454 200-209
AAAAG 1449010 1.2259054 14.823319 70-79
TATGG 2735725 1.2212251 5.6416335 180-189
AAAGT 1699315 1.2192707 20.495998 70-79
TCACG 2532965 1.2118396 9.845942 200-209
CTTCT 2569295 1.2051883 6.8543177 210-219
TGGAC 2795925 1.2017192 8.356303 170-179
GCATA 2048860 1.2004205 8.046936 220-229
GTGGA 3092685 1.1941936 13.451619 25-29
GGCTC 3386735 1.1886488 7.5455217 130-139
TTGGC 3245780 1.183143 8.368981 60-69
TAACT 1739265 1.1780701 5.9944706 240-247
GAAGA 1883845 1.1691955 17.323328 35-39
GGAGT 3017670 1.1652278 7.0308247 210-219
AAGTA 1622675 1.164281 16.310883 70-79
AGAGC 2282790 1.1569185 6.038855 140-149
AGCTT 2317905 1.151748 5.465541 150-159
GCTGA 2666865 1.1462477 11.399263 30-34
GGGAG 3409540 1.138808 5.1831684 150-159
ACTAT 1675045 1.1345714 5.8675156 230-239
AACTT 1654075 1.1203678 6.885403 210-219
CATGT 2252975 1.1194847 7.4404745 150-159
CTGCC 2843270 1.1107821 6.8079777 200-209
CCATG 2313245 1.1067195 7.523344 150-159
AGGTT 2474790 1.1047442 25.206251 10-14
AAGAG 1772120 1.0998541 12.970229 35-39
CAATA 1375850 1.0988435 6.868258 100-109
CTACC 2059180 1.0966011 7.829172 230-239
AGCGT 2534080 1.0891755 7.5707273 160-169
ATCCA 1668245 1.0879761 15.048186 240-247
TGCCC 2774000 1.0837204 5.771727 200-209
CGACT 2264790 1.0835373 6.627973 200-209
GTGGT 3307960 1.0832781 9.124709 50-59
TAGGC 2517090 1.0818728 7.4822392 160-169
ACGTG 2513165 1.0801859 6.855439 170-179
CGAAC 1913610 1.079515 13.345129 70-79
AAAAT 1102995 1.0788062 6.524365 90-99
TATCA 1592270 1.0785048 7.445993 70-79
CATCA 1650130 1.0761621 8.418402 230-239
TATAA 1294580 1.0738397 9.214363 240-247
CGGCT 3045505 1.0688866 6.687828 130-139
GTCCA 2230130 1.0669551 14.018864 70-79
TTCTG 2523515 1.0634288 6.150621 210-219
TTGAG 2382060 1.0633496 6.3127227 200-209
ATAAA 1084620 1.0608342 5.6408014 7
CTGAA 1802325 1.0559764 13.630441 35-39
GGAGG 3149600 1.0519866 9.934798 30-34
TCGAC 2172460 1.0393642 7.0245595 190-199
TGCGT 2850385 1.0390147 8.126018 210-219
CTCGG 2936130 1.0304991 7.6430893 130-139
TGGTC 2807880 1.023521 10.135431 50-59
TAGAT 1681660 1.023305 6.677788 240-247
CTTCC 2252355 1.0172598 6.9079576 230-239
ACTCG 2124120 1.0162369 6.705607 190-199
TATGT 1945910 1.0042235 6.3254676 220-229
CCAGC 2179375 1.0039269 7.077208 80-89
AGTTC 2011090 0.9992941 5.1108847 160-169
GGGGT 3526980 0.9990758 8.453494 10-14
AGTTT 1932925 0.9975224 5.5994377 220-229
ATGAT 1632740 0.9935368 10.568498 240-247
TGTAA 1631255 0.99263316 7.20498 230-239
ACTGG 2308890 0.99238616 5.482014 160-169
TCCCC 2276040 0.98975825 5.7811365 90-99
TCTAC 1783775 0.98659813 6.26071 180-189
CTCGA 2061560 0.9863066 6.327864 190-199
ACAAT 1233665 0.9852852 8.170795 100-109
GCCAC 2135180 0.98356843 5.278395 80-89
CTGCG 2784180 0.977169 6.2389836 210-219
AGAGA 1573880 0.9768179 11.956955 35-39
CGGAG 2613170 0.97154033 6.714182 210-219
GAGCT 2251940 0.96790844 5.4546146 140-149
GTCTA 1944040 0.96597743 6.108603 180-189
AGCTG 2230080 0.9585129 5.8288293 90-99
CCATT 1729970 0.95683885 5.812096 50-59
TACTG 1903520 0.94584346 5.407265 180-189
ACCCA 1501615 0.94291455 11.403559 240-247
TATTT 1577580 0.94120306 6.7164307 220-229
CATAC 1442640 0.94084376 15.788084 70-79
TACAA 1150935 0.91921157 6.3388977 100-109
TATGA 1496930 0.91089517 8.272268 240-247
TGCAT 1830415 0.9095182 6.416312 210-219
ACTAA 1114070 0.8897688 10.890358 240-247
CGTGG 2806670 0.8849632 6.4463973 50-59
GTATT 1695435 0.87496114 5.9995794 220-229
ATCAA 1084290 0.86598456 13.09589 70-79
ACGTC 1793740 0.8581742 9.531669 70-79
ATGGG 2200925 0.84985393 20.140759 15-19
AGCCA 1489435 0.8402273 7.6246033 80-89
TGAGA 1595415 0.83976215 5.0650125 240-247
CTATA 1230500 0.8334643 5.2282977 90-99
CCCAA 1310980 0.82320845 8.487864 240-247
ATTTA 1142815 0.8039466 5.6496744 220-229
TCGAA 1367785 0.8013808 15.974344 70-79
TTTAT 1329355 0.79310906 5.737972 220-229
CCGTA 1657435 0.79296213 7.5247035 80-89
TGAAG 1504470 0.79189235 8.918088 35-39
AAAGG 1246845 0.7738458 8.406045 10-14
GGTGG 2690600 0.7621572 13.961191 25-29
TAACC 1140195 0.7435988 6.568228 80-89
AGCAG 1461945 0.74091405 5.5451055 80-89
CCAAT 1120370 0.73066956 8.274479 240-247
GACCG 1755940 0.7266768 14.110508 45-49
CATTG 1455975 0.72346205 5.3230467 50-59
GTAGC 1677820 0.7211454 5.3672442 80-89
CATAT 1056365 0.7155161 6.183111 240-247
CTAAT 1039050 0.703788 8.539135 240-247
CCCAT 1316430 0.7010551 5.460767 240-247
ATCCC 1310135 0.69770277 5.512644 240-247
AAGGT 1318015 0.69375 10.025879 10-14
GCGTG 2193200 0.6915317 5.245945 160-169
CGTCC 1745330 0.68184924 8.041274 70-79
CCGAA 1200165 0.6770429 5.394691 90-99
AAAGC 978395 0.67591894 10.233406 70-79
TAGGG 1748125 0.67501205 5.005338 10-14
GAAAA 795720 0.6732027 6.150829 8
TAGGT 1485545 0.663146 6.191829 10-14
AATCC 1015885 0.6625278 8.048145 240-247
ATAGG 1257935 0.6621263 5.3921695 10-14
GAGGT 1689755 0.6524733 7.6588855 10-14
CATCC 1210100 0.64442986 8.269377 240-247
GAAGG 1403215 0.63888484 5.46041 10-14
GGTCC 1819210 0.6384916 10.203115 50-59
GAGGC 1713125 0.63691604 7.610006 30-34
GGGTC 1997075 0.6296921 5.739299 70-79
GTCGA 1428320 0.6139076 11.239068 70-79
CAATC 932415 0.6080913 7.9808893 240-247
AATGG 1130280 0.59493387 6.7824645 10-14
TACCG 1219525 0.583454 8.501657 70-79
GGGCC 1917070 0.582004 10.769888 15-19
TGGGC 1815520 0.5724465 11.568314 15-19
AAGGG 1246570 0.56756425 7.0206237 10-14
AGAGG 1241675 0.5653356 5.1972632 10-14
TGATC 1108135 0.5506231 6.571954 240-247
AAGCA 774235 0.5348761 6.4720454 70-79
ATAAG 736825 0.5286773 5.0799828 9
AGAAG 802835 0.49827403 5.3798633 9
TGGGT 1471490 0.48187792 5.573812 10-14
GATCC 1004040 0.48036015 6.483586 240-247
CGGTT 1312625 0.47847456 8.898691 10-14
TAAAA 487930 0.47722965 6.1348243 8
TAAGG 875175 0.46065688 6.184042 10-14
GGTCG 1407950 0.44393677 5.6886 70-79
CGATG 975820 0.41941816 8.602794 15-19
TAAAG 540200 0.38759744 5.231505 9