FastQCFastQC Report
Thu 4 May 2017
HCGCLBCXY_s2_1_BC_X.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s2_1_BC_X.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12131825
Filtered Sequences 0
Sequence length 251
%GC 53

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TCCCT 17567205 6.372171 102.3676 30-34
TAATA 10891915 6.12186 40.279694 110-119
CACAG 13612850 5.8798294 33.993366 110-119
ATACA 10460350 5.795904 27.710745 110-119
CAGTA 13179305 5.774473 35.21247 60-69
CCCTG 17566810 5.747996 71.46061 30-34
GGTGA 16115360 5.109141 89.456215 45-49
GGAGA 13974195 5.053923 79.331696 35-39
AGTAA 9800070 4.9687505 33.73711 110-119
GTAAT 10507945 4.670268 29.126417 110-119
ACTCC 10500010 4.3447857 112.321335 30-34
AATAC 7821525 4.3337755 30.009989 110-119
AGACG 10931370 4.3204937 82.71494 40-44
GTGAC 12437785 4.309311 72.069374 45-49
GTAGT 12143445 4.267867 20.240177 80-89
CTCAG 11167595 4.2284517 30.27263 140-149
ACAGT 9565330 4.191021 27.091259 110-119
ACCAG 9650375 4.1683087 77.76918 45-49
CCCCA 10113330 4.1254244 85.59754 60-69
CACTC 9941720 4.113772 92.31866 25-29
CTCCC 11306230 4.0429506 69.04519 30-34
GAGAC 9907715 3.9159062 116.836075 40-44
GGCCC 13020440 3.8431573 56.159054 60-69
ATGCA 8528290 3.736645 84.16227 25-29
TGACC 9845875 3.7280009 119.09547 45-49
GACCA 8392940 3.625182 87.46351 45-49
GCACA 8217050 3.5492098 26.852537 110-119
CGTGT 11657165 3.5404932 17.91734 120-129
CCCAG 9447585 3.5264547 75.43968 60-69
GTGTC 11598485 3.5226707 18.750002 120-129
TGCAC 9067620 3.433326 109.3139 25-29
GACGG 10981580 3.4321673 96.34396 40-44
AGTAG 8527245 3.418784 23.132057 60-69
TGAGG 10707505 3.3946586 61.7174 35-39
CCTGA 8877010 3.3611538 80.71303 30-34
AGGAG 9168320 3.315825 80.20993 35-39
CTGAG 9523190 3.2994938 92.47331 35-39
GGATG 10386405 3.292859 68.79509 20-24
ACGGT 9470390 3.2811997 82.0626 40-44
CAGGG 10496695 3.2806222 34.109318 50-59
GGGTT 11799115 3.2791655 40.145702 50-59
GCACT 8612860 3.261138 84.99388 25-29
ATGGT 9236275 3.2461295 20.331432 190-199
TAGTA 7263925 3.2284598 22.956331 80-89
GCCCC 9862135 3.181196 67.39572 60-69
GAGGA 8641930 3.1254501 104.746414 35-39
AAAAA 4255985 3.112914 8.127185 120-129
TACAC 6448510 3.0877078 22.081415 110-119
GGTTG 11044755 3.0695167 75.39113 15-19
TGTCC 9231230 3.0639899 16.903313 120-129
CGGAT 8737910 3.0274181 76.485405 20-24
GATGC 8708070 3.017079 92.30364 25-29
GTTGG 10675980 2.9670281 54.281082 15-19
CCACT 7121985 2.946997 20.068182 240-247
CTCTC 8051400 2.920493 11.937439 100-109
CCTGG 9743715 2.917368 26.582376 50-59
CAGAT 6630710 2.9052258 14.653058 150-159
CCAGG 8498980 2.9028687 37.130783 50-59
AGGGT 9148275 2.9003277 46.716072 50-59
TGGTG 10421865 2.8964052 15.746013 190-199
AGATA 5651120 2.865184 14.418696 150-159
AGGCT 8256615 2.8606641 17.01808 140-149
TCAGG 8121050 2.813695 18.086578 140-149
CCGTG 9289960 2.781509 18.894308 120-129
TGGCC 9249780 2.7694786 40.607086 60-69
GCGGA 8788820 2.7468455 89.52667 20-24
TCTTG 8136735 2.7395685 14.29669 170-179
TTCCC 7511285 2.724576 43.256535 50-59
CTTGG 8917125 2.7082934 12.453611 170-179
GGCGG 10936150 2.7027948 47.94034 20-24
CTTCA 6359300 2.6692681 12.342961 200-209
CGGTG 9691270 2.6551583 63.851692 40-44
CCCTT 7304435 2.649545 15.189997 20-24
TCTCA 6308050 2.6477563 17.37162 130-139
TCGCA 6931910 2.62467 18.629139 100-109
GGTTC 8626750 2.6201012 44.256344 50-59
CGCAC 7001740 2.6135066 19.757156 100-109
TCAAA 4640800 2.5713892 41.578854 70-79
TTGGG 9250210 2.5707834 81.181915 15-19
CCAGT 6776215 2.565718 34.053234 60-69
AGTCA 5797320 2.5400784 26.595045 70-79
GCCGT 8379495 2.5089068 17.988453 120-129
TCTCT 6773580 2.4923394 11.789977 180-189
TGTCT 7369825 2.4813561 15.509376 180-189
GGCTG 8960080 2.4548311 12.039444 140-149
TGGGG 9767955 2.4488173 57.979317 15-19
ACAGC 5614315 2.4250042 10.534962 120-129
GTTCC 7250600 2.4065878 48.922268 50-59
CAGCT 6331485 2.3973272 11.238046 150-159
CGGCC 8100770 2.3910508 10.428153 120-129
ACCAC 5046615 2.3821702 12.611788 230-239
TCCAT 5646475 2.3700652 13.453123 70-79
GATGG 7309865 2.3174863 11.409615 190-199
GCCCT 7071765 2.3139367 13.586823 200-209
CTGGC 7726000 2.3132439 32.452946 60-69
TGTTC 6830175 2.2996607 11.158577 150-159
TACCA 4773745 2.2857883 13.299316 230-239
TTCTT 6098850 2.2763603 15.297425 170-179
TCCTC 6272675 2.275294 14.826812 130-139
TCAGC 6007980 2.2748365 12.750504 140-149
AGATG 5618020 2.2524035 13.575751 180-189
GGGGC 9008605 2.2264154 54.675014 15-19
AACCA 4056450 2.2157304 11.970817 70-79
GGGCG 8802705 2.1755285 67.54371 20-24
GCTGT 7083395 2.151356 9.233139 140-149
CACGG 6215175 2.1228237 8.977258 120-129
GAGAT 5269420 2.112641 13.081747 180-189
ACTAC 4404410 2.1089416 11.063421 240-247
GACTC 5537215 2.0965881 13.545004 190-199
CATTT 4820335 2.0524106 13.878694 150-159
CTGGA 5889435 2.040509 11.0029335 180-189
TCATT 4779045 2.0348303 13.690489 150-159
TGAAT 4562510 2.0278127 11.661838 190-199
GTCAA 4619250 2.0239105 31.947027 70-79
CAGGC 5886995 2.0107324 14.717296 140-149
TCTGC 6038745 2.0043542 10.169516 210-219
GTTCT 5946015 2.0019717 13.738958 160-169
TTCAT 4682335 1.9936528 12.968213 140-149
TAGTC 5186775 1.9921559 24.824389 70-79
ACAGA 3974165 1.9863681 7.9365263 140-149
CTACT 4721550 1.9818349 7.6409583 230-239
CAAAA 3134485 1.981231 15.854615 70-79
TGGAG 6242695 1.9791555 11.072354 180-189
ATTTG 5073630 1.9767369 12.437743 150-159
CTCGC 6022240 1.970524 14.102276 100-109
TTGTC 5852565 1.970508 11.814586 170-179
CAGCC 5221385 1.9489616 10.026188 120-129
AGCTC 5118950 1.938218 11.240913 140-149
CTCTG 5791530 1.9222996 11.934103 180-189
AATAA 2997670 1.9220157 6.811652 80-89
ATCTC 4553750 1.9114021 6.4110746 100-109
GTCCT 5730160 1.9019299 12.925997 130-139
ACACA 3473980 1.8975714 12.0206585 120-129
GATAC 4329625 1.8970124 11.829248 150-159
TGGAA 4705215 1.8864373 8.160353 170-179
TACAG 4283810 1.8769386 11.902827 160-169
ACCAT 3850855 1.8438857 10.012256 45-49
TTCAC 4386030 1.8410028 13.629697 200-209
TACTA 3771980 1.832105 9.3713665 230-239
ACTCT 4351425 1.8264775 10.0574045 200-209
GAATC 4165565 1.8251299 11.931195 190-199
TCGGC 6092250 1.8240823 6.9015093 190-199
GTTCA 4738290 1.8199002 11.856863 140-149
TTGGA 5152765 1.810962 12.487929 170-179
TCACA 3757940 1.7993957 6.9384403 140-149
TTTGC 5337290 1.7970195 10.537831 150-159
GTGAA 4474810 1.7940623 13.263086 190-199
TCTCG 5397410 1.791485 13.303701 100-109
TTGCA 4637025 1.7810059 11.563039 150-159
CCTTC 4896495 1.7761106 10.789952 200-209
CGTAG 5067330 1.7556746 8.914704 80-89
AGGGA 4852135 1.7548286 5.3677435 160-169
ACGGC 5131930 1.7528361 11.642706 120-129
AATAT 3114360 1.750443 7.6336126 110-119
AATCG 3984520 1.7458056 11.393906 190-199
CACTA 3629585 1.7379359 10.798268 240-247
TGTAG 4937375 1.7352622 5.4640026 210-219
GCAGA 4372255 1.7280817 11.866117 150-159
CTGTT 5124975 1.7255349 10.6686945 140-149
CATAG 3917240 1.7163271 6.4831877 210-219
CCATA 3535180 1.6927325 15.69587 70-79
ACACG 3871830 1.6723685 11.474999 120-129
GTCTC 5025705 1.6681103 9.6085415 180-189
GATAT 3742015 1.6631428 5.7186904 180-189
TGCTC 5007380 1.662028 6.0522494 150-159
AGTAT 3726080 1.6560605 10.124926 220-229
TGCAG 4761100 1.6495752 11.4016285 150-159
CACCA 3488185 1.6465394 8.758382 230-239
CTGTG 5360675 1.6281348 5.205108 210-219
TGGTT 5275485 1.6253144 17.317266 10-14
CTCCA 3914535 1.6197907 7.429793 150-159
ATATG 3624140 1.6107533 5.7689276 180-189
ATTGT 4121405 1.6057405 6.7076716 170-179
CTGCT 4800630 1.5934045 5.775021 140-149
GTGCT 5218350 1.5849078 6.329559 220-229
CAGAG 4000360 1.5810945 6.0394793 140-149
TCAGA 3589195 1.5725952 8.387631 130-139
GAGTC 4537320 1.5720423 9.434737 210-219
TGTGC 5165910 1.5689809 7.0950937 220-229
GCTCT 4704520 1.5615039 8.999458 130-139
CCAGA 3595610 1.55306 20.04888 60-69
CAAAG 3083970 1.5414307 22.19973 70-79
TCTGG 5059915 1.5367885 9.612811 180-189
CTGGT 5047100 1.5328963 5.1335206 160-169
AAAAT 2384015 1.5285585 5.1402035 90-99
GAACT 3483620 1.5263379 6.451134 160-169
TTCAG 3914510 1.5034996 6.9065933 150-159
GCTCC 4534655 1.4837744 6.756476 150-159
GTCTG 4834230 1.4682436 7.5423455 210-219
ATAGT 3299315 1.4663844 6.1907053 220-229
GCTCA 3867990 1.4645597 9.158043 140-149
CCATC 3524645 1.4584583 11.2253475 240-247
CCTCA 3501935 1.4490613 8.008795 130-139
ATATA 2577870 1.4489058 6.953794 240-247
AGTCT 3752690 1.4413471 9.783929 210-219
CCACC 3492815 1.4247874 7.029765 230-239
ATAAC 2564985 1.421215 7.3673153 80-89
TGACT 3663655 1.4071503 8.831661 190-199
ACTTC 3337525 1.4009008 6.1626573 210-219
ATACC 2920035 1.3981858 11.422982 70-79
ATGTA 3141500 1.3962432 6.1866446 160-169
GTCCC 4242550 1.3881954 16.955275 50-59
GACGT 4003715 1.3871645 6.6173916 60-69
ACCGT 3657000 1.3846713 14.953274 45-49
GTCAC 3628125 1.3737382 5.4418197 140-149
TGATA 3082845 1.3701739 5.380083 180-189
CCTTG 4113160 1.3652221 10.014571 20-24
GGCCG 4997930 1.3498819 9.940178 120-129
GAACC 3119090 1.3472358 10.246432 70-79
GCGTA 3834850 1.328658 8.523862 210-219
ACTGA 3031555 1.3282669 5.614106 180-189
TTGGT 4293010 1.3226256 10.802559 25-29
AGCCG 3869175 1.3215358 8.993511 120-129
GAGAA 2884305 1.3191619 6.4182773 160-169
CTGAT 3420850 1.313893 5.7473335 180-189
CAATA 2365895 1.3109027 5.61546 100-109
CCTCG 3992900 1.306508 7.480715 130-139
GTGTT 4208960 1.2967306 6.6500793 160-169
TAGTT 3316485 1.2921356 7.9092374 220-229
AACTG 2947670 1.2915128 6.2606654 160-169
ATCAT 2658135 1.2910945 5.243642 230-239
GTAGG 4055950 1.2858799 5.374819 160-169
AAAAG 2216905 1.2822098 11.2250805 70-79
AGAAC 2546055 1.2725698 6.6313243 160-169
AATAG 2504450 1.2697856 5.891273 240-247
CATCA 2636265 1.2623096 6.469629 230-239
AATTG 2836905 1.2608658 7.865721 170-179
TCCCA 3045815 1.2603241 8.182594 240-247
CTCTT 3425230 1.2603136 5.851045 200-209
ATCGG 3584590 1.2419506 7.1813416 190-199
TTGGC 4078845 1.2388195 7.8770385 60-69
CTTCT 3351445 1.2331644 5.7544003 210-219
CAGCG 3588965 1.2258288 6.5961695 160-169
TCTGT 3611555 1.2159795 6.134231 210-219
TCCAC 2922200 1.2091736 7.474444 240-247
ACGGA 3051720 1.2061559 7.5957894 200-209
TTCCA 2866865 1.2033447 7.225389 230-239
TGGTC 3947555 1.1989442 10.073974 50-59
GAATT 2686965 1.1942247 8.503081 170-179
TTCTG 3540875 1.192182 5.4340143 210-219
GCTGA 3440230 1.1919342 9.090478 30-34
TCACG 3138840 1.1884774 7.898612 200-209
CTTGA 3076225 1.1815281 5.6552444 200-209
TTGTA 3022205 1.1774812 5.4079356 220-229
TATCA 2423880 1.1773134 5.8648996 70-79
AGATC 2683365 1.1757084 6.316317 130-139
GAGGG 4105530 1.1741295 5.176989 200-209
CCCAC 2871340 1.1712756 8.220711 240-247
GTGGT 4213335 1.1709541 9.095413 50-59
GGCTC 3891515 1.1651595 6.7047963 130-139
GGAAT 2903355 1.1640271 7.616102 170-179
AAAGT 2280435 1.1562073 15.560583 70-79
CAGAC 2666010 1.1515359 11.560779 60-69
AGGTT 3258030 1.1450491 21.66558 10-14
ATATC 2356970 1.1448144 6.0695615 70-79
CATAC 2390050 1.1444157 14.15509 70-79
GATCT 2966785 1.1394938 5.2597723 130-139
CCAGC 3002575 1.1207569 6.010286 80-89
GCATA 2556370 1.1200659 6.144455 220-229
GGAGT 3521775 1.1165276 6.0010824 210-219
CTGCC 3406105 1.1145042 5.941986 200-209
TGCCC 3402655 1.1133753 5.466453 60-69
TGGAC 3210070 1.1121907 7.071769 170-179
AAGAG 2428530 1.1107092 9.949803 35-39
CCATG 2889830 1.0941931 6.090027 150-159
ATAGA 2157815 1.0940377 6.392899 240-247
AGAGC 2753245 1.0881872 5.184014 140-149
GAAGA 2372330 1.0850056 13.593641 35-39
AGCGT 3127340 1.0835273 6.272792 160-169
ATCCA 2235590 1.0704564 11.09742 240-247
GTCCA 2820405 1.0679065 13.049844 70-79
AAGTA 2103430 1.0664637 11.953572 70-79
TCCCC 2974455 1.0636238 5.096952 90-99
CGGCT 3527050 1.056035 6.09115 130-139
CTACC 2546145 1.0535662 6.7410455 230-239
TGCGT 3448940 1.0475059 6.95351 210-219
AACTT 2149620 1.0441015 5.117808 210-219
TTGAG 2968700 1.0433626 5.234824 200-209
CGAAC 2406250 1.0393372 10.588678 70-79
CTCGG 3444360 1.0312768 6.9343557 130-139
GGAGG 3605645 1.0311687 7.347942 30-34
GTGGA 3248015 1.0297358 9.343002 25-29
TATAA 1797850 1.0104913 6.567854 240-247
AGCTG 2915950 1.0102873 5.0798206 90-99
GGGGT 4011375 1.0056481 8.689066 10-14
ACGTG 2901735 1.0053623 5.7412634 170-179
TATTT 2310005 0.9977095 5.31483 220-229
CTTCC 2742695 0.99486065 5.7927756 230-239
CTGAA 2267565 0.993527 11.610645 35-39
ATCAA 1789200 0.9913657 9.838246 70-79
AGAGA 2161425 0.9885464 9.518902 35-39
CTGCG 3268685 0.978678 5.666032 210-219
ACAAT 1762005 0.9762974 6.221154 100-109
TAACC 2032015 0.9729796 5.5743985 80-89
TAGGC 2804120 0.9715415 6.2292266 160-169
CGACT 2564410 0.97097766 5.3252954 200-209
CGGAG 3087645 0.9650084 5.8884277 210-219
CATGT 2478730 0.9520399 5.8526864 150-159
TATGT 2429730 0.94664705 5.2013783 220-229
CGTGG 3448045 0.9446755 6.576008 50-59
GTCTA 2444925 0.9390559 5.0282955 180-189
AAAGC 1862170 0.9307502 8.1113 70-79
ATGAT 2085870 0.9270673 7.8136835 240-247
TCTAC 2196885 0.9221259 5.041439 180-189
ACTCG 2401845 0.9094246 5.402783 190-199
AAGCA 1805510 0.9024304 5.5014505 70-79
GTATT 2314110 0.90160036 5.0333424 220-229
AAAGG 1921265 0.8787071 7.5488935 10-14
TCGAC 2316150 0.8769775 5.4674234 190-199
ACTAA 1555700 0.86198723 8.270393 240-247
TGCAT 2236940 0.8591723 5.0037484 210-219
AGCCA 1987980 0.85867274 5.966931 80-89
TGTAA 1892780 0.8412482 5.1734295 230-239
TGAAG 2073385 0.8312714 7.719703 35-39
TAGAT 1855200 0.82454574 5.149472 240-247
AAGGT 2040825 0.81821734 9.154286 10-14
CCGTA 2145495 0.81236124 6.7066913 80-89
CGTCC 2465255 0.80665064 7.9997954 70-79
GGTGG 3190215 0.79978406 10.400481 25-29
ACGTC 2087665 0.7904648 8.924771 70-79
ATGGG 2491150 0.7897828 14.904309 15-19
CCAAT 1644290 0.7873271 6.1661944 240-247
ACCCA 1657570 0.7824282 8.579634 240-247
TATGA 1746420 0.7761984 6.0926776 240-247
CAATC 1609235 0.77054197 5.895326 240-247
TCGAA 1740445 0.7625709 12.515513 70-79
CCCAA 1603650 0.7569762 6.213173 240-247
CTAAT 1555315 0.755439 6.6503925 240-247
GACCG 2189230 0.74774235 14.487629 45-49
GAGGC 2360560 0.73776615 5.7001 30-34
AATCC 1523570 0.7295234 5.7807655 240-247
GAAGG 2005025 0.72513956 5.182411 10-14
TACCG 1885070 0.7137551 8.163984 70-79
CATCC 1704445 0.7052801 6.9884763 240-247
AATGG 1751990 0.70241624 5.922862 10-14
GGTCC 2300790 0.6888801 10.616516 50-59
GGGTC 2444540 0.669741 5.0530033 70-79
GAGGT 2102810 0.6666654 6.8869367 10-14
TAGGT 1805380 0.6345087 5.1385293 10-14
CGGTT 2039705 0.6194956 8.250226 10-14
GGTCG 2125460 0.5823213 5.1161895 70-79
GTCGA 1670860 0.57890177 9.393148 70-79
GGGCC 2079080 0.5615349 8.495803 15-19
TGGGC 2023435 0.55436903 9.107456 15-19
AAGGG 1517935 0.5489781 6.0958514 10-14
TAAGG 1281185 0.5136588 5.1941853 10-14
TGGGT 1782390 0.49535507 5.3415775 10-14
CGATG 1294685 0.44856867 6.223105 15-19
GATCC 1092045 0.41348734 5.0187373 240-247