FastQCFastQC Report
Thu 4 May 2017
HCGCLBCXY_s1_1_illumina12index_7_SL248371.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s1_1_illumina12index_7_SL248371.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2554674
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 3043065 8.577278 57.69131 110-119
ATACA 3081725 8.306999 38.098022 110-119
CACAG 3976245 7.5691643 43.487244 110-119
TCCCT 4873900 7.118045 117.055954 30-34
CAGTA 3564605 7.0953555 41.972374 60-69
AGTAA 2750940 6.817554 47.556103 110-119
GGAGA 3974715 6.395509 100.416794 35-39
GTAAT 3013185 6.2715735 39.275932 110-119
GGTGA 4609120 6.228599 109.358345 45-49
CCCTG 4699680 6.0347824 79.83967 30-34
AATAC 2153675 5.8053775 41.143333 110-119
GTAGT 3673755 5.646422 33.86528 80-89
ACAGT 2756490 5.4868007 35.62652 110-119
AGACG 3111855 5.4461718 104.37557 40-44
CTCAG 3225900 5.1573777 37.85775 140-149
GTGAC 3461755 5.088286 87.84383 45-49
ACTCC 2884865 5.0165644 131.95619 30-34
TAGTA 2377815 4.949129 42.37613 80-89
GAGAC 2735895 4.7881904 146.2779 40-44
CCCCA 2808100 4.6698775 98.62779 60-69
CACTC 2630835 4.5748253 108.47999 25-29
ACCAG 2375980 4.5229063 89.10015 45-49
GCACA 2347695 4.469063 34.250412 110-119
ATGCA 2232730 4.444255 108.41814 25-29
AGTAG 2373110 4.3428793 32.457043 80-89
CGTGT 3470640 4.28438 21.388212 120-129
CTCCC 3055080 4.2669625 75.84858 30-34
GGCCC 3754905 4.239372 59.935326 60-69
TGACC 2638635 4.2184935 144.24158 45-49
AGATA 1667500 4.1325045 21.16742 150-159
GTGTC 3335630 4.1177154 22.150637 120-129
CCCAG 2607180 3.9862182 88.33399 60-69
TACAC 1824975 3.9511409 28.261812 110-119
ATGGT 2549935 3.9191535 26.281895 190-199
CAGAT 1951220 3.8839085 19.89914 150-159
AGGAG 2393480 3.8512256 101.472374 35-39
TGCAC 2404750 3.8445714 130.49268 25-29
TGAGG 2841210 3.839509 73.465866 35-39
GACCA 2014325 3.8344612 102.57445 45-49
GACGG 2932770 3.7902024 114.74271 40-44
ACGGT 2537675 3.7300203 100.27558 40-44
CTGAG 2533675 3.7241414 109.291084 35-39
GGATG 2754345 3.7221224 83.881065 20-24
GAGGA 2305445 3.709573 131.71199 35-39
CCTGA 2316770 3.7039146 97.33081 30-34
TGTCC 2701620 3.627484 19.968744 120-129
GCACT 2253050 3.6020424 101.085304 25-29
AGGCT 2372830 3.487722 21.615118 140-149
GGGTT 3053280 3.4653113 42.711628 50-59
CGGAT 2357315 3.4649165 92.59209 20-24
CAGGG 2676815 3.4594157 36.76827 50-59
CCCTT 2353215 3.436732 20.78194 50-59
CCACT 1966645 3.4198484 25.112427 240-247
CTCTC 2321275 3.3900855 13.455519 130-139
GCCCC 2739560 3.3642335 72.81826 60-69
CCGTG 2849380 3.363883 21.822266 120-129
TCAGG 2282730 3.3552878 22.63075 140-149
CTTCA 1843290 3.3516793 16.331852 200-209
GATGC 2258250 3.3193054 111.25987 25-29
AAAAA 826295 3.3019226 18.312502 130-139
TCTCA 1800815 3.2744465 22.316816 130-139
GGTTG 2853020 3.2380266 86.3915 15-19
TGGTG 2842190 3.2257352 18.54558 190-199
CTTGG 2587495 3.1941693 14.908321 170-179
TCTTG 2268750 3.185341 17.600721 170-179
TCGCA 1950985 3.1191194 22.419024 100-109
GTTGG 2738785 3.108376 62.193615 15-19
ACAGC 1630165 3.1031759 13.399917 120-129
CGCAC 2011970 3.0761788 23.24247 100-109
CCTGG 2580895 3.0469186 27.54496 50-59
CCAGG 2159155 3.0350864 39.63101 50-59
AGGGT 2221085 3.0014946 52.179184 50-59
GCGGA 2311945 2.9878712 105.39894 20-24
TCCAT 1632165 2.9677882 22.40954 70-79
TGTCT 2066930 2.9019837 19.057404 180-189
TCTCT 1893110 2.8909988 14.822302 180-189
AGATG 1574545 2.8814762 18.821396 180-189
TGGCC 2439230 2.8796732 44.358154 60-69
CGGTG 2641025 2.8665543 72.83206 40-44
ACCAC 1366280 2.8288984 16.611568 230-239
AGTCA 1408455 2.8035333 30.618662 70-79
GCCGT 2348245 2.7722595 20.473198 120-129
GGCTG 2533700 2.7500646 14.075223 140-149
TGTTC 1955065 2.7449245 14.409581 150-159
GATGG 2026385 2.7383838 14.852641 190-199
CGGCC 2420725 2.7330525 11.273219 120-129
CAGCT 1708765 2.731872 13.151729 150-159
CCAGT 1700035 2.717915 38.317413 60-69
TTCCC 1860780 2.7175596 44.714584 50-59
ACTAC 1253330 2.7135074 15.6457405 240-247
TTGGG 2390155 2.7126994 93.836075 15-19
GAGAT 1475085 2.6994605 17.94081 180-189
GCCCT 2093970 2.6888325 16.457306 200-209
GGTTC 2176555 2.686879 46.69825 50-59
GGCGG 2812865 2.6843908 52.158882 20-24
TCAAA 988710 2.6651351 53.842903 70-79
TCCTC 1823320 2.6628516 17.96997 130-139
TTCTT 1655055 2.6428497 19.724968 170-179
TACCA 1200770 2.599713 17.525839 230-239
CACGG 1817440 2.5547435 10.355009 120-129
CTGGA 1732145 2.5460064 14.321551 180-189
TGGGG 2505225 2.4999514 64.42742 15-19
ACACA 960850 2.476954 16.154688 120-129
TCATT 1299970 2.4716682 18.464289 150-159
GATAC 1237660 2.4635653 16.24567 150-159
GCTGT 1993305 2.460663 11.21821 140-149
TACAG 1234360 2.456997 16.80322 160-169
GTGAA 1341540 2.455068 18.539703 190-199
CATTT 1289620 2.4519894 18.650967 150-159
TCAGC 1520780 2.431333 14.940445 140-149
TGAAT 1166320 2.4275513 16.029182 190-199
ATTTG 1386690 2.424004 16.837616 150-159
CGTAG 1640705 2.4116023 13.548477 80-89
GTTCC 1792470 2.406762 51.087444 50-59
TTGTC 1710695 2.4018273 14.439999 170-179
TTCAT 1260610 2.3968318 17.069355 140-149
GACTC 1495830 2.3914444 15.910783 190-199
ACAGA 1004835 2.3815203 9.91196 140-149
TTCAC 1306235 2.375145 18.205414 200-209
TGGAG 1748915 2.363421 14.106845 180-189
CAGGC 1679955 2.3614833 18.119297 140-149
AATCG 1177260 2.343339 15.931134 190-199
GTTCT 1663355 2.3353617 16.840063 160-169
GAATC 1168565 2.3260317 16.30824 190-199
TACTA 1023535 2.3171613 13.270113 240-247
TGGAA 1256665 2.2997437 11.0126 170-179
CTACT 1256835 2.2853205 9.715739 230-239
GGGGC 2355230 2.2476578 59.491657 15-19
CTCTG 1667975 2.2396016 14.744984 180-189
AGCTC 1389330 2.2211785 13.405135 140-149
GTCCT 1637045 2.1980717 15.890029 130-139
GGGCG 2295580 2.1907322 72.74364 20-24
TCTGC 1631010 2.1899686 12.410434 210-219
CTGGC 1854485 2.1893432 33.001087 60-69
CAGCC 1430310 2.1868563 11.950665 120-129
AGTAT 1046000 2.1771202 14.874408 220-229
GTTCA 1300560 2.1741843 15.0801735 140-149
TTTGC 1548280 2.1737957 13.105829 150-159
TAGTC 1289540 2.155762 27.896555 70-79
AGGGA 1338195 2.1532207 6.656821 160-169
ACTCT 1176355 2.1389825 12.095846 200-209
TTGCA 1272470 2.1272256 14.967162 150-159
ATCTC 1169885 2.127218 8.002044 100-109
CATAG 1058870 2.1076837 8.758415 210-219
CTCGC 1629975 2.0930245 15.527052 100-109
AATAA 622830 2.0902805 8.75728 80-89
TCGGC 1769655 2.0891957 8.310761 190-199
TCACA 963780 2.0866206 8.18581 140-149
TTGGA 1354710 2.0821378 15.389251 170-179
CACTA 957120 2.0722015 14.801118 240-247
AACCA 790435 2.0376446 13.791646 80-89
CCAGA 1069885 2.036629 31.990513 60-69
ACACG 1068110 2.03325 14.14868 120-129
GATAT 972390 2.02391 6.9492483 180-189
CCTTC 1385405 2.0233026 13.503838 200-209
ATATG 967205 2.013118 7.2520714 180-189
CCATA 923390 1.9991747 26.940979 70-79
GCAGA 1134545 1.9856086 15.641699 150-159
GTCTC 1462100 1.9631718 12.10142 180-189
TGTAG 1272020 1.9550465 6.581236 150-159
TCTCG 1453225 1.9512553 15.573969 100-109
GAGTC 1326890 1.9503391 12.842281 210-219
CTGTT 1378215 1.9350234 13.387657 140-149
GAACT 968210 1.9272246 7.985426 160-169
AATAT 683380 1.9261961 10.560056 110-119
ATTGT 1099910 1.9226983 8.882966 50-59
TGCAG 1307950 1.9224999 14.051804 150-159
ACCGT 1190075 1.9026213 24.355307 45-49
CAGAG 1082920 1.8952581 7.1354065 140-149
ACGGC 1342740 1.8874661 13.546454 120-129
CAAAA 578625 1.8571385 23.07992 70-79
ATATA 657370 1.8528836 9.797202 240-247
AGTCT 1091915 1.8253865 13.500843 210-219
GTCAA 912515 1.8163633 36.256306 70-79
CTCCA 1043535 1.8146293 8.667143 150-159
CTGTG 1444625 1.7833378 5.8961687 210-219
ATAGT 853215 1.7758619 8.531243 220-229
ACCAT 819920 1.7751583 13.180522 45-49
CACCA 852805 1.7657425 10.9247 230-239
ATGTA 845570 1.7599498 8.032793 160-169
GACGT 1188295 1.7466241 11.036932 60-69
TGTGC 1412990 1.7442853 8.407924 220-229
ATACC 798685 1.7291837 19.856607 70-79
TCTGG 1391725 1.7180344 11.782679 180-189
GTCTG 1390785 1.716874 9.4858885 210-219
TCAGA 857950 1.7077519 10.504271 130-139
TGCTC 1266680 1.70078 6.4933257 150-159
GCTCT 1259120 1.6906292 10.984361 130-139
GTCCC 1303925 1.6743487 24.09529 50-59
GTGCT 1355245 1.673001 7.211104 220-229
AGATC 824565 1.6412988 8.310053 130-139
GCGTA 1081600 1.5897977 10.783864 210-219
AATTG 761255 1.5844585 10.907941 170-179
ATCGG 1069515 1.5720347 9.411654 190-199
CAGAC 823625 1.5678494 19.721987 60-69
GTTGT 1206090 1.5568477 5.222366 210-219
GCTCC 1211570 1.5557572 7.439323 150-159
GAACC 813270 1.5481377 11.267524 70-79
TGACT 921975 1.5412928 10.374127 190-199
GGAAT 842060 1.541001 10.323704 170-179
TTCAG 913335 1.526849 7.611275 150-159
TAGTT 873000 1.526048 9.683358 220-229
CAAAG 643255 1.5245537 26.189735 70-79
GAATT 731405 1.5223296 11.88707 170-179
TGGTT 1171240 1.5118625 20.50775 10-14
AGCCG 1066950 1.4997929 10.79109 120-129
CCTCA 861750 1.4985188 9.57115 130-139
CCATC 859795 1.4951191 13.830305 240-247
ACTTC 817585 1.4866261 7.529169 210-219
CCACC 888970 1.4783593 8.302979 230-239
GTCAC 923345 1.4761891 6.168486 140-149
GCTCA 921385 1.4730556 10.238558 140-149
ACGGA 837785 1.4662383 10.218557 200-209
ATCAT 643765 1.4574072 6.5754666 240-247
GTAGG 1073120 1.4501758 6.418706 160-169
TGATA 696105 1.4488568 6.427663 180-189
CCTCG 1118675 1.4364724 8.800022 130-139
CCTTG 1069050 1.4354209 11.771885 20-24
CTGCT 1063855 1.4284457 5.972921 140-149
GTGTT 1105390 1.4268619 8.1166725 160-169
CTGGT 1153995 1.4245653 5.2795486 160-169
AACTG 715430 1.4240652 7.650856 160-169
AGAAC 595220 1.4107077 8.51089 160-169
GGCCG 1353260 1.4046915 10.900906 120-129
ATAAC 515665 1.39001 8.892258 80-89
TTGGC 1124715 1.3884202 10.99897 60-69
GGACG 1062110 1.3726311 5.211135 170-179
TCCCA 789010 1.3720294 9.801053 240-247
GAGAA 629215 1.3710577 8.126717 160-169
ACTGA 683450 1.3604091 6.566188 180-189
GATCT 811115 1.3559649 6.678004 130-139
GGTCA 905130 1.330412 5.04148 130-139
GTCCA 826385 1.3211752 21.04208 70-79
CATAC 608125 1.3166139 24.899837 70-79
TTGTA 751280 1.3132752 6.4911942 220-229
AATAG 523700 1.2978666 8.157691 240-247
CACTG 808905 1.2932295 5.1858416 230-239
CCCAC 774665 1.2882699 9.772355 240-247
TCACG 803470 1.2845403 9.94215 200-209
GTGGT 1118320 1.2692341 14.614994 50-59
CAGCG 902195 1.2681997 8.145202 160-169
GGAGT 928550 1.2548089 7.9232354 210-219
TGGTC 1005785 1.2416054 15.986902 50-59
CTGAT 742345 1.2410002 6.540173 180-189
GGCTC 1050065 1.2396717 8.0433655 130-139
AAAAG 418925 1.2361765 17.098116 70-79
ATATC 544935 1.2336677 8.556879 70-79
TTCCA 676190 1.2295256 8.947264 230-239
TATGG 799225 1.2283787 5.3910365 190-199
GCATA 616915 1.2279708 8.0592 220-229
ATAGA 494690 1.2259722 8.279356 240-247
TATAC 540930 1.224601 5.2474704 110-119
TCTGT 867715 1.2182778 7.0792875 210-219
TCCAC 698350 1.2143784 8.335015 240-247
TAACT 535825 1.2130438 6.293421 240-247
AAATA 359720 1.2072567 5.077225 9
TGCCC 928695 1.192522 7.4897366 60-69
GAGGG 992390 1.1791363 5.82087 200-209
AGCGT 788685 1.1592544 8.043095 160-169
TGGAC 788400 1.1588355 8.055303 170-179
GAAGA 530495 1.1559471 20.28683 35-39
CTACC 659950 1.1476036 8.5437355 230-239
AGGTT 743820 1.1432232 26.856178 10-14
AGCTT 681160 1.1387153 5.2972956 150-159
CTCTT 745625 1.138656 6.216926 200-209
TTGGT 879695 1.1355298 12.85341 25-29
CGGCT 961515 1.1351324 7.162018 130-139
CTTGA 678950 1.1350207 6.2589355 200-209
TGCGT 908590 1.1216216 8.464849 210-219
CTCGG 949770 1.1212668 8.162336 130-139
GCTGA 752795 1.1065012 10.8771925 30-34
CATGT 656050 1.0967382 7.1194935 150-159
CTGCC 853525 1.0959976 6.4440637 200-209
TATCA 482690 1.0927526 8.353065 70-79
CGTGG 1003965 1.0896982 10.14247 50-59
AACTT 480845 1.0885756 6.669297 210-219
AAGTA 438435 1.0865574 14.817459 70-79
CTTCT 709915 1.0841227 6.3118896 210-219
ACTAT 477955 1.0820332 5.7015595 230-239
AGAGC 617795 1.0812256 5.9034123 140-149
ACGTC 671075 1.072875 14.556994 70-79
TATAA 380470 1.0724046 9.574845 240-247
AAAGT 432050 1.0707337 18.44791 70-79
CGGAG 827515 1.0694493 7.6824865 210-219
CGAAC 560975 1.0678698 11.591003 70-79
TAGGC 724870 1.0654556 7.394136 160-169
GTGGA 783500 1.0587938 11.741307 25-29
AAGAG 485415 1.0577179 15.460272 35-39
CCGTA 660470 1.0559204 10.279672 70-79
AAAAT 313640 1.0526074 5.926054 90-99
CCATG 657810 1.0516677 7.1751075 150-159
CAATA 389665 1.0503685 6.575983 100-109
CATCA 484230 1.0483766 8.095513 230-239
ACGTG 711400 1.0456566 6.613212 170-179
TTGAG 670645 1.0307559 6.1125565 200-209
AGTTC 615400 1.0287822 5.146173 160-169
TATGT 587975 1.02781 6.8590727 220-229
CTGCG 865775 1.0221051 6.7725215 210-219
TCCCC 731075 1.0210762 5.927794 90-99
AGAGA 467590 1.0188773 14.362348 35-39
ATCCA 468975 1.0153489 13.833495 240-247
CGACT 634650 1.0146407 6.129606 200-209
CTGAA 507480 1.0101404 15.697114 35-39
TGCTA 597830 0.99941 5.0367117 230-239
TCGAC 620955 0.9927461 6.3748174 190-199
TCATA 435500 0.9859201 5.271138 240-247
TATTT 493940 0.9820169 6.797577 220-229
AGCTG 664045 0.97605145 5.9437733 90-99
AGTTT 554165 0.96870834 5.630221 220-229
TTCTG 689710 0.9683576 5.9339714 210-219
ACTCG 603095 0.96419257 5.9803157 190-199
TGTAA 463130 0.96394795 6.8829823 230-239
GAGCT 654645 0.96223485 5.2226624 140-149
ATGAT 462300 0.9622205 10.550017 240-247
CTTCC 657100 0.9596559 6.840714 230-239
GACCG 680925 0.95716435 22.295427 45-49
ACTGG 649130 0.9541286 5.3189206 160-169
CTCGA 593430 0.9487407 5.718104 190-199
TAGAT 453770 0.94446635 6.442538 240-247
TCTAC 516265 0.93873173 5.8416486 180-189
ACAAT 347120 0.93568563 7.711817 100-109
GGGGT 935175 0.93320644 8.963993 10-14
GTATT 533200 0.9320605 6.4074287 220-229
ACTAA 344885 0.92966104 12.232512 240-247
CCAGC 606185 0.9268197 6.443066 80-89
CCATT 508795 0.92514896 6.607328 50-59
TACTG 553045 0.92454165 5.261911 180-189
TACAA 342430 0.92304343 6.1046057 100-109
GTCTA 547405 0.91511303 5.7347293 180-189
TGCAT 545845 0.91250515 6.313744 210-219
GGAGG 761320 0.90458375 8.900422 30-34
ACCCA 434225 0.8990678 11.22917 240-247
ATCAA 331655 0.8939987 14.573422 70-79
CGTCC 694900 0.8923097 12.045742 70-79
AAAGA 298050 0.8794949 5.888991 8
CCCCC 657445 0.87814784 5.2178326 80-89
TATGA 420455 0.87512535 8.480694 240-247
TACCG 537460 0.85925925 13.546105 70-79
TGAAG 467365 0.85529524 10.442266 35-39
GGTCC 709605 0.83773595 15.7284775 50-59
TTTAT 420850 0.8367045 5.9089613 220-229
ATTTA 344820 0.81627023 5.627691 220-229
TGAGA 442265 0.8093614 5.313668 240-247
AGCCA 406945 0.7746589 6.88243 80-89
CCCAA 372605 0.7714829 7.2991204 240-247
GCGTG 695955 0.7553858 5.594137 160-169
TATTA 316730 0.7497746 5.1479206 230-239
ATGGG 552870 0.74712867 17.475279 15-19
TCGAA 373450 0.74335325 14.568306 70-79
CATTG 442875 0.74036723 6.1816173 50-59
AAAGG 339760 0.74033606 9.516415 10-14
AAAGC 311295 0.7377881 11.409238 70-79
GTAGC 500660 0.7358988 5.3536434 80-89
CCGAA 380685 0.7246705 6.053982 90-99
CTAAT 317415 0.7185897 9.256821 240-247
AAGGT 391980 0.7173379 11.308311 10-14
CATAT 315575 0.7144242 6.217231 240-247
CCTTT 464270 0.7089942 5.017161 50-59
TAACC 327250 0.7085088 5.9474907 80-89
AGCAG 393545 0.68875754 5.076388 80-89
TAGGG 504585 0.68187803 5.1822367 10-14
AGAAA 225075 0.66415817 5.371518 7
ATCCC 381110 0.66272175 5.1838813 240-247
CCCAT 378865 0.6588179 5.315693 240-247
CCAAT 303285 0.65662366 7.254329 240-247
ATAGG 356710 0.6527925 5.41414 10-14
CCCGA 417650 0.6385613 5.074696 90-99
TAGGT 414595 0.6372168 6.315349 10-14
AATCC 286400 0.620067 6.859592 240-247
GGTGG 616820 0.6155216 12.034066 25-29
AATGG 328915 0.6019266 6.9699483 10-14
GAGGC 457785 0.5916243 6.950371 30-34
CATCC 338385 0.5884262 7.873271 240-247
GAAAA 197525 0.5828628 6.3190045 8
AAGCA 243950 0.5781763 7.5041056 70-79
GAGGT 427465 0.5776609 8.086774 10-14
GAAGG 357495 0.57522684 6.1052327 10-14
GTCGA 379915 0.55842084 9.920568 70-79
CAATC 255055 0.55220383 6.7914076 240-247
AAGGG 337175 0.5425309 6.9211435 10-14
GGGTC 497530 0.5400164 5.005152 70-79
TGATC 314825 0.5263022 6.1787434 240-247
ATAAG 207600 0.5144875 5.7171507 9
GGGCC 490520 0.5091625 9.010749 15-19
AGAGG 307645 0.49501574 5.384305 10-14
AAGAA 165795 0.48923287 5.9436536 9
TGGGC 443760 0.48165476 9.815943 15-19
CGGTT 384220 0.47430575 9.489235 10-14
TAAAA 139285 0.46745452 6.1589847 8
TTGCC 343700 0.4614884 5.446354 60-69
GATCC 287300 0.45931822 6.171833 240-247
AGAAG 207430 0.4519894 5.3846703 9
TAAGG 244915 0.4482035 6.309566 10-14
GGTCG 380125 0.41258562 5.0176177 70-79
TGGGT 351285 0.39868987 5.632278 10-14
CGATG 257760 0.37887043 7.32044 15-19
TAAAG 150450 0.37285474 5.6100307 9