FastQCFastQC Report
Thu 4 May 2017
HCGCLBCXY_s2_1_illumina12index_8_SL248372.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s2_1_illumina12index_8_SL248372.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9373162
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 10815775 8.295979 57.026844 110-119
ATACA 10647645 7.860789 39.240067 110-119
CACAG 14078760 7.330537 42.904785 110-119
TCCCT 17365600 7.205231 116.750755 30-34
CAGTA 13042540 7.055551 45.2974 60-69
AGTAA 9581085 6.3611274 45.89558 110-119
CCCTG 17489495 6.2812324 78.11011 30-34
GGAGA 14222865 5.9892435 93.95679 35-39
GGTGA 16686235 5.94826 102.635 45-49
GTAAT 10576605 5.94446 38.0849 110-119
AATAC 7607185 5.6161227 41.38222 110-119
ACAGT 9582300 5.183684 34.80515 110-119
ACTCC 10412905 5.103678 132.98949 30-34
GTAGT 12206190 5.026924 23.032368 70-79
CTCAG 11373775 5.0132895 37.25325 140-149
GTGAC 12483630 4.9484105 84.207085 45-49
AGACG 10491735 4.9127584 99.42633 40-44
ACCAG 9182050 4.7809153 97.16013 45-49
CCCCA 9943495 4.690867 99.164696 60-69
CACTC 9475155 4.6440587 108.4357 25-29
GAGAC 9903530 4.6373315 138.89944 40-44
GCACA 8541110 4.4471903 34.008804 110-119
CTCCC 10808510 4.3164487 76.38135 30-34
ATGCA 7945390 4.298174 105.170784 25-29
GGCCC 13732190 4.2689066 61.04137 60-69
TGACC 9408445 4.147018 141.07034 45-49
CGTGT 12279510 4.1205263 21.366611 120-129
GACCA 7897345 4.1119943 108.87067 45-49
GTGTC 12091200 4.0573363 22.57048 120-129
TAGTA 7035440 3.9541886 26.109356 80-89
CCCAG 9308080 3.9489431 86.82827 60-69
AGTAG 8061000 3.92161 29.566849 60-69
TACAC 6457800 3.8846028 29.344048 110-119
AAAAA 3623835 3.8786886 21.866652 130-139
TGCAC 8603850 3.7923725 127.79773 25-29
AGATA 5638890 3.7438028 20.226084 150-159
TGAGG 10491075 3.7398274 69.874054 35-39
ATGGT 9051370 3.727662 25.539406 190-199
AGGAG 8843140 3.723843 94.87437 35-39
CAGGG 10795265 3.703971 39.10219 50-59
GACGG 10572595 3.6275704 108.097404 40-44
GGATG 10160970 3.622153 78.33626 20-24
CTGAG 9103385 3.6085086 105.93242 35-39
CCACT 7357795 3.606277 25.551014 240-247
CCTGA 8155440 3.594724 93.845276 30-34
GGGTT 11912055 3.5947196 45.66795 50-59
ACGGT 9032375 3.5803614 95.46326 40-44
GCACT 8085790 3.5640233 98.79364 25-29
TGTCC 9541530 3.5602698 20.420341 120-129
GAGGA 8434410 3.551727 123.789894 35-39
CAGAT 6461440 3.4954097 18.569248 150-159
CTTCA 6639970 3.3812313 16.007708 200-209
CGGAT 8450365 3.3496568 88.36915 20-24
GCCCC 9663455 3.340432 72.6384 60-69
AGGCT 8397105 3.3285453 20.729464 140-149
CTCTC 7988595 3.3145802 13.691933 100-109
CCAGG 8676405 3.3103 43.04134 50-59
CCTGG 10182250 3.2886527 30.280838 50-59
TGGTG 10784445 3.2544389 18.05092 190-199
TCAGG 8181255 3.2429838 22.190586 140-149
GATGC 8062980 3.1961005 107.19171 25-29
AGGGT 8906960 3.1751273 55.287804 50-59
CCCTT 7642360 3.1709228 21.977604 20-24
TCTCA 6216670 3.1656768 22.540537 130-139
TCTTG 8087675 3.1353493 17.164122 170-179
GGTTG 10380330 3.1324885 82.2123 15-19
AGTCA 5711795 3.0898783 34.118626 70-79
TTCCC 7440760 3.0872757 51.164803 50-59
CCAGT 6914010 3.0475311 42.140556 60-69
CCGTG 9422140 3.043153 21.680586 120-129
TCGCA 6901490 3.0420127 23.19551 100-109
GTTGG 10036015 3.028584 59.150715 15-19
CTTGG 9010690 3.0236368 14.445479 170-179
CGCAC 7090180 3.008001 23.96959 100-109
TGGCC 9142890 2.9529612 45.087936 60-69
GGTTC 8690720 2.9162676 50.986176 50-59
ACAGC 5509195 2.8685308 12.920981 120-129
TGTCT 7382175 2.8618484 18.68629 180-189
GCGGA 8306385 2.8500092 99.37266 20-24
TCAAA 3831635 2.828764 56.90399 70-79
ACCAC 4875835 2.8230178 15.613577 230-239
TCTCT 6479730 2.7932665 14.306876 180-189
CGGTG 9467145 2.7497928 68.81578 40-44
TCCAT 5394825 2.7471738 15.046877 70-79
GCCGT 8364950 2.701703 20.334625 120-129
CAGCT 6106045 2.691399 13.249972 150-159
GCCCT 7485870 2.6884987 16.082067 200-209
GATGG 7532940 2.6853206 13.485124 190-199
AGATG 5514970 2.6829872 17.844263 180-189
GTTCC 7139300 2.663916 57.00475 50-59
GGCTG 9107655 2.6453767 13.41495 140-149
ACTAC 4387290 2.6391153 14.366093 230-239
TTCTT 5843455 2.6171136 19.31905 170-179
TCCTC 6303025 2.6152136 17.598211 130-139
CGGCC 8398610 2.6108642 11.517974 120-129
TTGGG 8629635 2.6041787 88.67479 15-19
TACCA 4302950 2.5883818 16.996466 230-239
GGCGG 10225355 2.5708025 49.071495 20-24
TGTTC 6604770 2.5604713 13.424341 140-149
GAGAT 5124400 2.492978 17.107061 180-189
TCAGC 5650170 2.4904604 15.01569 140-149
TGAAT 4420280 2.4843678 15.5074835 190-199
GACTC 5626780 2.4801507 17.115543 190-199
CACGG 6402880 2.442884 10.546488 120-129
TGGGG 9193485 2.4014184 59.786537 15-19
CTGGA 6036245 2.3927193 13.504166 180-189
CTGGC 7404835 2.391606 36.240906 60-69
GCTGT 7065940 2.371055 10.488925 140-149
ACAGA 3708340 2.3697445 10.254084 140-149
GAATC 4378940 2.368851 15.787862 190-199
TCACA 3933550 2.366174 9.0212 140-149
TCATT 4456730 2.3578844 17.380133 150-159
ACACA 3268200 2.3223298 15.722646 120-129
TAGTC 5068720 2.3212066 31.193888 70-79
CATTT 4381985 2.3183396 17.539558 150-159
CTACT 4549280 2.316602 9.475887 230-239
TTCAT 4311245 2.2809136 17.48794 140-149
TGGAG 6377110 2.2732933 13.089701 180-189
TTCAC 4451565 2.266843 17.531784 200-209
GTTCT 5842545 2.2649798 17.415586 160-169
TACAG 4153125 2.2466931 15.263305 160-169
TACTA 3594645 2.2465463 12.324813 230-239
TTGTC 5793670 2.2460322 14.38899 170-179
GATAC 4151545 2.2458384 15.6228 150-159
ATTTG 4711110 2.2414865 15.659645 150-159
GTGAA 4604610 2.2401044 17.17487 190-199
CAGGC 5836880 2.2269387 17.391975 140-149
TGGAA 4576000 2.226186 10.523577 170-179
ACTCT 4361410 2.2209342 12.96496 190-199
TCTGC 5938390 2.2158153 11.973122 210-219
AGGGA 5246160 2.209156 6.870045 150-159
AGCTC 5009020 2.207857 13.836459 140-149
ATCTC 4300630 2.1899834 8.512327 100-109
CAGCC 5107755 2.1669595 11.637421 120-129
GGGGC 8515245 2.1408563 55.492252 15-19
AATCG 3957205 2.1407075 15.245769 190-199
AACCA 3012260 2.1404629 15.618128 80-89
CTCTG 5735335 2.1400487 14.102253 180-189
ACACG 4078220 2.12345 14.544768 120-129
GTCCT 5683010 2.1205244 15.043638 130-139
AATAA 2329250 2.1104708 9.305725 80-89
CACTA 3505055 2.1084187 14.2731495 240-247
CTCGC 5846800 2.099838 16.332487 100-109
GGGCG 8305470 2.0881157 68.48758 20-24
GTTCA 4507155 2.0640395 15.287782 140-149
CGTAG 5198480 2.0606358 10.101879 210-219
TTGGA 4961350 2.0432527 15.108471 170-179
GAACT 3772175 2.0406125 8.42293 160-169
GATAT 3602280 2.0246205 7.6137724 180-189
CATAG 3722465 2.0137215 8.656082 210-219
CTCCA 4102990 2.010999 9.525243 150-159
ATATG 3562410 2.002212 7.836515 180-189
TGTAG 4852640 1.9984822 6.777371 150-159
GTCAA 3681740 1.9916906 40.416985 70-79
TTGCA 4331585 1.9836377 14.143261 150-159
CCTTC 4754310 1.9726299 13.264757 200-209
AGTAT 3505955 1.9704821 13.254524 220-229
TCTCG 5272215 1.9672428 16.329773 100-109
TTTGC 4983220 1.9318453 12.358608 150-159
TCGGC 5963805 1.9261837 8.097354 190-199
AATAT 2479640 1.9019481 10.0819435 110-119
CAGAG 4029195 1.886672 7.3138876 140-149
GTCTC 5040185 1.8806647 11.434335 180-189
ACGGC 4853440 1.8517278 13.486999 120-129
ATATA 2398785 1.8399299 9.91465 240-247
CAAAA 2107605 1.8380406 20.976889 70-79
ATTGT 3824670 1.8197296 8.526075 170-179
CTGTG 5418795 1.8183371 6.0295615 210-219
CTGTT 4685475 1.8164185 12.61652 140-149
TGCAG 4558045 1.8067725 13.340954 150-159
GCAGA 3854170 1.8047165 14.408722 150-159
GAGTC 4539155 1.7992846 11.685731 210-219
TGCTC 4706695 1.7562282 6.731683 150-159
TGTGC 5184490 1.7397132 8.097278 220-229
GTCTG 5139315 1.7245544 9.121716 210-219
ATGTA 3058940 1.7192422 8.29829 150-159
ACCAT 2851070 1.7150228 11.537069 45-49
AGTCT 3744785 1.7149142 12.624943 210-219
TCAGA 3168480 1.7140352 10.990776 130-139
ATAGT 3010805 1.6921886 8.235453 220-229
GTGCT 5001240 1.6782217 7.4488144 220-229
CAAAG 2622450 1.6758269 29.58256 70-79
TTCAG 3650790 1.6718693 8.399336 140-149
CACCA 2884485 1.6700631 10.775686 230-239
GCTCC 4553340 1.6353008 7.785711 150-159
GTCAC 3647480 1.6077224 6.6854644 140-149
TCTGG 4788130 1.6067101 11.134119 180-189
CCATA 2657865 1.5988029 17.891077 70-79
TGACT 3479370 1.593368 10.842649 190-199
GCTCT 4247015 1.5847058 10.609397 130-139
AGATC 2901825 1.5697842 8.63391 130-139
ACTTC 3035955 1.5459808 7.726452 210-219
CTGCT 4135015 1.5429147 6.1070933 140-149
TAGTT 3230815 1.5371809 10.149001 220-229
GAACC 2945335 1.5335786 13.279471 70-79
CCATC 3128220 1.5332348 13.659648 240-247
GTTGT 4393030 1.5315568 5.444 210-219
CCACC 3244315 1.530513 8.144814 230-239
CCAGA 2934715 1.5280491 22.349457 60-69
GCTCA 3437675 1.5152453 10.96979 140-149
TGGTT 4336690 1.5119148 18.698933 10-14
CCTCA 3076215 1.5077456 9.465439 130-139
ATCGG 3803285 1.5075916 9.112647 190-199
TGATA 2655715 1.4926142 6.8800898 180-189
GACGT 3754590 1.4882895 7.039873 170-179
AATTG 2635920 1.4814888 10.639059 170-179
GGAAT 3044315 1.481034 9.790106 170-179
GCGTA 3715650 1.4728538 10.340977 210-219
AACTG 2716215 1.4693758 7.895946 160-169
TCCCA 2997135 1.4689862 10.354694 240-247
CTGGT 4377530 1.4689289 5.726777 160-169
AGAAC 2269685 1.4503993 8.995397 160-169
GTAGG 4019910 1.4330057 6.407754 160-169
GAATT 2543255 1.4294074 11.353299 170-179
GTCCC 3975545 1.4277896 17.419239 50-59
AGCCG 3704715 1.413456 10.276264 120-129
CTTGA 3080200 1.4105692 6.750279 200-209
GAGAA 2435085 1.3994031 8.425229 160-169
TTGTA 2937325 1.3975418 6.848161 210-219
GGACG 4071395 1.3969392 5.178923 170-179
ATAAC 1881785 1.3892571 9.63535 80-89
GGTCA 3495900 1.3857467 5.4989376 130-139
ACTGA 2560610 1.3851991 6.671354 180-189
GTGTT 3968705 1.383623 8.049226 160-169
CCTTG 3707260 1.3833048 14.398174 20-24
GGCCG 4929115 1.3780106 10.904642 120-129
CACTG 3109215 1.370468 5.213625 230-239
CCTCG 3802280 1.3655626 8.411717 130-139
ACCGT 3082590 1.3587321 15.516873 45-49
ATACC 2256460 1.3573432 12.799906 70-79
AATAG 2043465 1.3567086 8.2177 240-247
ACGGA 2886600 1.3516514 9.716721 200-209
CCCAC 2864795 1.3514737 9.508499 240-247
ATCAT 2155045 1.3468391 6.982854 230-239
GATCT 2931460 1.3424542 6.7457557 130-139
TCCAC 2729095 1.3376117 9.391485 240-247
TTCCA 2585330 1.3165118 9.343125 230-239
CTGAT 2861545 1.3104368 6.819239 180-189
ATAGA 1973705 1.3103932 8.843634 240-247
GAGGG 4111345 1.2686006 6.2116094 200-209
TTGGT 3633685 1.2668238 15.183776 25-29
AAATA 1388590 1.258164 5.8769226 9
TCTGT 3205795 1.2427907 7.340963 210-219
CAGCG 3236965 1.2349958 7.6047983 160-169
ATATC 1971630 1.2322102 7.841711 70-79
CTCTT 2849590 1.2283945 6.789067 200-209
CAGAC 2353470 1.2254062 12.722283 60-69
AAAGT 1844660 1.2247169 20.585464 70-79
TATGG 2973700 1.2246709 5.6703935 180-189
AAAAG 1552590 1.2176694 14.82099 70-79
TCACG 2748550 1.2114954 9.830978 200-209
TGGAC 3046870 1.2077547 8.357957 170-179
GCATA 2226245 1.2043195 8.050794 220-229
CTTCT 2783535 1.1999197 6.8491 210-219
GTGGA 3329240 1.1867976 13.394605 25-29
GGCTC 3673485 1.1864583 7.520819 130-139
TAACT 1894565 1.1840469 6.0327454 240-247
TTGGC 3521720 1.1817523 8.369159 60-69
GAAGA 2028860 1.1659522 17.286087 35-39
GGAGT 3269565 1.165525 7.0455694 210-219
AAGTA 1754865 1.1650996 16.316452 70-79
AGAGC 2478210 1.1604227 6.0568166 140-149
AGCTT 2517255 1.1527702 5.5039024 150-159
GCTGA 2895040 1.1475707 11.395382 30-34
GGGAG 3695250 1.1402099 5.208939 150-159
ACTAT 1821435 1.1383427 5.9039493 230-239
AACTT 1797060 1.1231091 6.931699 210-219
CATGT 2448580 1.1213206 7.46639 150-159
CTGCC 3090630 1.1099784 6.821499 200-209
AGGTT 2692650 1.1089249 25.283474 10-14
CCATG 2511340 1.106939 7.5546865 150-159
CAATA 1491385 1.1010381 6.939129 100-109
CTACC 2245820 1.100744 7.9093823 230-239
AAGAG 1907500 1.0962087 12.967263 35-39
ATCCA 1814980 1.0917768 15.048742 240-247
AGCGT 2753380 1.0914178 7.6065974 160-169
CGAAC 2084430 1.0853223 13.461064 70-79
ACGTG 2735270 1.084239 6.86168 170-179
TGCCC 3015670 1.0830572 5.7764115 200-209
TAGGC 2732285 1.0830559 7.4985557 160-169
CGACT 2456050 1.0825683 6.6410475 200-209
GTGGT 3583595 1.081427 9.137868 50-59
TATCA 1724930 1.07803 7.448155 70-79
TATAA 1403430 1.076467 9.253681 240-247
CATCA 1787590 1.0753007 8.4165125 230-239
AAAAT 1185340 1.0740047 6.4472704 90-99
GTCCA 2424690 1.0687456 14.065142 70-79
CGGCT 3302100 1.0665089 6.661484 130-139
TTGAG 2582555 1.063584 6.3297496 200-209
ATAAA 1171675 1.0616232 5.697885 7
TTCTG 2733050 1.0595216 6.126199 210-219
CTGAA 1951215 1.055538 13.57185 35-39
GGAGG 3388055 1.0454215 9.918678 30-34
TCGAC 2355930 1.0384378 7.0109634 190-199
TGCGT 3088965 1.0365366 8.097836 210-219
CTCGG 3184620 1.0285653 7.603346 130-139
TGGTC 3052115 1.0241711 10.146038 50-59
TAGAT 1819395 1.0225703 6.679558 240-247
CTTCC 2458205 1.0199437 6.8980083 230-239
ACTCG 2307520 1.0170999 6.706719 190-199
TATGT 2119120 1.0082505 6.3199 220-229
CCAGC 2365745 1.0036649 7.081384 80-89
GGGGT 3823690 0.9987812 8.397447 10-14
AGTTT 2095680 0.99709797 5.58238 220-229
ATGAT 1773980 0.9970453 10.644917 240-247
AGTTC 2175645 0.9963308 5.109199 160-169
ACTGG 2511600 0.99557805 5.483811 160-169
TGTAA 1765905 0.9925067 7.2274156 230-239
TCCCC 2480530 0.9906158 5.7898192 90-99
TCTAC 1944915 0.9903971 6.304256 180-189
ACAAT 1335870 0.98622674 8.248307 100-109
CTCGA 2235725 0.9854544 6.338348 190-199
GCCAC 2316435 0.982745 5.27017 80-89
CTGCG 3024515 0.97685474 6.226377 210-219
CGGAG 2834725 0.97262436 6.7455835 210-219
AGAGA 1690740 0.9716403 11.982305 35-39
GAGCT 2448565 0.9705916 5.465013 140-149
GTCTA 2112580 0.9674503 6.1586027 180-189
AGCTG 2418475 0.95866406 5.8340755 90-99
CCATT 1873050 0.9538019 5.7874346 50-59
TACTG 2072200 0.9489584 5.4155073 180-189
TATTT 1715405 0.9429099 6.692704 220-229
ACCCA 1627760 0.9424427 11.396448 240-247
CATAC 1563315 0.9403911 15.843672 70-79
TACAA 1250355 0.923094 6.4110446 100-109
TGCAT 1987865 0.91033745 6.467298 210-219
TATGA 1618405 0.90960604 8.334436 240-247
ACTAA 1208200 0.89197236 11.01266 240-247
CGTGG 3039735 0.88291055 6.4473033 50-59
GTATT 1841210 0.8760243 6.003174 220-229
ATCAA 1171440 0.8648337 13.063611 70-79
ACGTC 1943335 0.85657585 9.547506 70-79
ATGGG 2387210 0.85098565 20.095562 15-19
AGCCA 1618145 0.8425367 7.607533 80-89
TGAGA 1719525 0.8365346 5.0460873 240-247
CTATA 1335940 0.8349228 5.2504478 90-99
CCCAA 1420560 0.82247794 8.516859 240-247
ATTTA 1241750 0.8062894 5.636888 220-229
TCGAA 1485265 0.8034756 16.052177 70-79
TTTAT 1443805 0.7936189 5.734683 220-229
CCGTA 1799780 0.7933002 7.4999247 80-89
TGAAG 1625150 0.790622 8.889352 35-39
AAAGG 1341710 0.77105856 8.410254 10-14
GGTGG 2905325 0.7588962 13.898559 25-29
TAACC 1235480 0.74318635 6.5752378 80-89
AGCAG 1580415 0.7400299 5.56678 80-89
CCAAT 1216210 0.73159474 8.308089 240-247
GACCG 1902800 0.72597325 14.176292 45-49
CATTG 1575355 0.7214296 5.304543 50-59
GTAGC 1818010 0.72064453 5.380811 80-89
CATAT 1148410 0.7177221 6.224453 240-247
CTAAT 1127640 0.7047415 8.589967 240-247
CCCAT 1423310 0.69760716 5.4292355 240-247
AAGGT 1433020 0.6971524 10.123296 10-14
ATCCC 1421725 0.6968303 5.553476 240-247
GCGTG 2386785 0.693257 5.258719 160-169
CGTCC 1898960 0.68199843 8.034712 70-79
CCGAA 1304225 0.6790846 5.4358172 90-99
TAGGG 1896365 0.67601067 5.024243 10-14
AAAGC 1055865 0.6747306 10.245796 70-79
ATAGG 1363475 0.66331935 5.34457 10-14
AATCC 1102205 0.6630166 8.016963 240-247
GAAAA 842985 0.6611385 6.1601567 8
TAGGT 1601605 0.65959543 6.170956 10-14
GAGGT 1825280 0.6506705 7.6951165 10-14
CATCC 1318195 0.64608705 8.267639 240-247
GAAGG 1520780 0.6403998 5.477293 10-14
GGTCC 1979170 0.63923025 10.211986 50-59
GAGGC 1857540 0.6373418 7.6110773 30-34
GGGTC 2175845 0.6319881 5.7664003 70-79
GTCGA 1556110 0.6168296 11.306286 70-79
CAATC 1007465 0.606027 7.9677463 240-247
AATGG 1220175 0.5936051 6.80454 10-14
TACCG 1326065 0.5844979 8.524777 70-79
GGGCC 2074630 0.57999504 10.710504 15-19
TGGGC 1963645 0.5703532 11.535239 15-19
AAGGG 1344745 0.56627154 6.9958453 10-14
AGAGG 1338335 0.56357235 5.2153945 10-14
TGATC 1204215 0.551467 6.626363 240-247
AAGCA 835485 0.53390086 6.4700527 70-79
ATAAG 799115 0.53055286 5.181193 9
AGAAG 861340 0.4949979 5.321526 9
CGGTT 1430495 0.4800185 8.963345 10-14
GATCC 1088435 0.47975624 6.5166473 240-247
TGGGT 1589185 0.4795708 5.5620055 10-14
TAAAA 518350 0.469663 6.153313 8
TAAGG 953955 0.46409124 6.253717 10-14
GGTCG 1537520 0.44658253 5.716934 70-79
CGATG 1054090 0.41783282 8.603052 15-19
TAAAG 585005 0.38839978 5.421435 9