FastQCFastQC Report
Thu 4 May 2017
HCGCLBCXY_s2_1_illumina12index_7_SL248371.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s2_1_illumina12index_7_SL248371.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2742288
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 3276775 8.6223755 57.99908 110-119
ATACA 3313970 8.339229 38.189526 110-119
CACAG 4276305 7.591965 43.74568 110-119
CAGTA 3834395 7.118448 42.24253 60-69
TCCCT 5237580 7.1095243 116.82415 30-34
AGTAA 2950720 6.8303022 47.783936 110-119
GGAGA 4272820 6.419031 100.64263 35-39
GTAAT 3235745 6.281534 39.409782 110-119
GGTGA 4947295 6.2330723 109.503334 45-49
CCCTG 5052040 6.0326524 79.60674 30-34
AATAC 2316520 5.829259 41.21704 110-119
GTAGT 3936590 5.6379704 33.685448 80-89
ACAGT 2963520 5.501693 35.867184 110-119
AGACG 3340125 5.454851 104.67029 40-44
CTCAG 3468015 5.1635213 37.899685 140-149
GTGAC 3717575 5.09166 87.88534 45-49
ACTCC 3098210 5.0146565 132.12378 30-34
TAGTA 2542140 4.9350424 42.05539 80-89
GAGAC 2937900 4.7979665 146.75504 40-44
CCCCA 3020040 4.6745534 98.668335 60-69
CACTC 2828920 4.5787935 108.2773 25-29
ACCAG 2559530 4.544078 89.437454 45-49
GCACA 2523090 4.4793835 34.547337 110-119
ATGCA 2397040 4.450038 108.57963 25-29
AGTAG 2545530 4.347117 32.198032 80-89
CGTGT 3727750 4.281796 21.350803 120-129
CTCCC 3287795 4.267874 75.74261 30-34
GGCCC 4037790 4.241475 59.923637 60-69
TGACC 2832430 4.2172 144.20654 45-49
AGATA 1790815 4.145364 21.151163 150-159
GTGTC 3585315 4.118191 22.08702 120-129
CCCAG 2800355 3.987265 88.37089 60-69
TACAC 1961120 3.9578373 28.27509 110-119
ATGGT 2738520 3.9220989 26.40878 190-199
CAGAT 2093245 3.8860514 19.90716 150-159
AGGAG 2567825 3.8576276 101.76918 35-39
TGCAC 2584630 3.848251 130.45921 25-29
TGAGG 3053970 3.847681 73.762245 35-39
GACCA 2166535 3.8463714 102.93864 45-49
GACGG 3151845 3.797486 114.791046 40-44
ACGGT 2725740 3.7332244 100.164635 40-44
CTGAG 2725390 3.7327447 109.641174 35-39
GGATG 2960810 3.7303097 83.80341 20-24
GAGGA 2476485 3.7204082 132.28156 35-39
CCTGA 2487695 3.7039251 97.12831 30-34
TGTCC 2898955 3.6198115 19.900942 120-129
GCACT 2418980 3.6016154 100.974266 25-29
AGGCT 2551740 3.4949107 21.694105 140-149
CAGGG 2886530 3.4778225 37.058037 50-59
CGGAT 2534805 3.4717162 92.55003 20-24
GGGTT 3284110 3.4700165 42.893524 50-59
CCCTT 2528200 3.431795 20.546309 50-59
CCACT 2118040 3.4281871 25.13808 240-247
CTCTC 2502015 3.3962512 13.383666 100-109
GCCCC 2942895 3.360573 72.745926 60-69
TCAGG 2453525 3.3603938 22.689358 140-149
CCGTG 3057280 3.3582373 21.879457 120-129
CTTCA 1984010 3.3579724 16.456585 200-209
GATGC 2424410 3.3205175 111.37866 25-29
TCTCA 1936795 3.2780602 22.145788 130-139
GGTTG 3065395 3.2389205 86.489334 15-19
TGGTG 3051980 3.224746 18.592035 190-199
CTTGG 2779360 3.1924489 15.0682745 170-179
TCTTG 2443735 3.1908147 17.803999 170-179
AAAAA 835680 3.1265204 17.138094 130-139
TCGCA 2095820 3.1204627 22.435312 110-119
ACAGC 1752270 3.1109035 13.576615 120-129
GTTGG 2942605 3.1091795 62.194843 15-19
CGCAC 2164265 3.081573 23.169493 100-109
CCTGG 2777900 3.0513556 27.559624 50-59
CCAGG 2327850 3.0489595 39.942482 50-59
AGGGT 2386085 3.0062165 52.527283 50-59
GCGGA 2484360 2.99327 105.52609 20-24
TCCAT 1748505 2.9593766 22.294197 70-79
TGTCT 2225780 2.906228 19.222887 180-189
TCTCT 2035540 2.8892987 14.846568 180-189
AGATG 1688030 2.8827255 18.794676 180-189
TGGCC 2621250 2.879285 44.36815 60-69
CGGTG 2835350 2.8649533 72.62247 40-44
ACCAC 1467565 2.8323586 16.594404 230-239
AGTCA 1515055 2.8126578 30.790663 70-79
GCCGT 2522800 2.7711434 20.51775 120-129
GGCTG 2721970 2.7503896 14.129465 140-149
TGTTC 2100845 2.743099 14.485056 150-159
CAGCT 1837840 2.736357 13.276246 150-159
GATGG 2171535 2.7359061 14.891099 190-199
CGGCC 2601030 2.7322383 11.272468 120-129
CCAGT 1833150 2.7293737 38.493973 60-69
ACTAC 1351510 2.7275522 15.756942 240-247
TTCCC 2007945 2.7255971 44.8963 50-59
TTGGG 2564755 2.7099402 93.76803 15-19
GAGAT 1582025 2.7016962 17.966661 180-189
GGTTC 2345860 2.694519 46.874508 50-59
GCCCT 2250495 2.6873214 16.504236 200-209
GGCGG 3022375 2.686525 52.197807 20-24
TCAAA 1064820 2.6794982 54.044666 70-79
TCCTC 1961620 2.6627154 17.995035 130-139
TTCTT 1783730 2.6475544 19.905418 170-179
TACCA 1292500 2.608461 17.565458 230-239
CTGGA 1864135 2.553154 14.411869 180-189
CACGG 1949160 2.5529613 10.328705 120-129
TGGGG 2691235 2.5014806 64.31514 15-19
ACACA 1028625 2.4753203 16.335093 120-129
TCATT 1398595 2.4753022 18.555786 150-159
GATAC 1331015 2.4709926 16.173937 150-159
GTGAA 1444140 2.4662235 18.63273 190-199
TACAG 1327240 2.4639843 16.920214 160-169
CATTT 1389715 2.4595861 18.812613 150-159
GCTGT 2140215 2.4583097 11.279059 140-149
TGAAT 1255800 2.4378777 16.154032 190-199
TCAGC 1631815 2.4296064 14.81621 140-149
ATTTG 1490495 2.4266183 16.942364 150-159
GTTCC 1930185 2.4101465 51.23498 50-59
CGTAG 1757120 2.4065843 13.520822 80-89
GACTC 1612735 2.4011984 16.02466 190-199
TTCAT 1355365 2.3987916 17.014626 140-149
TTGTC 1836945 2.3985214 14.346402 170-179
ACAGA 1079470 2.389569 10.019726 140-149
TTCAC 1404505 2.3771498 18.322605 200-209
TGGAG 1878805 2.3670967 14.139839 180-189
CAGGC 1807000 2.3667634 18.168482 140-149
AATCG 1264140 2.3468409 15.95055 190-199
GTTCT 1791240 2.338844 16.79001 160-169
GAATC 1257120 2.3338084 16.4036 190-199
TACTA 1104145 2.3301446 13.363618 240-247
TGGAA 1351450 2.3079326 11.11401 170-179
CTACT 1352380 2.2889276 9.750167 230-239
GGGGC 2529880 2.248757 59.394875 15-19
CTCTG 1794765 2.2410529 14.821971 180-189
AGCTC 1493405 2.2235281 13.44964 140-149
CTGGC 1998950 2.195726 33.102234 60-69
GTCCT 1757980 2.195121 15.900029 130-139
GGGCG 2464555 2.1906908 72.82322 20-24
CAGCC 1535495 2.1863036 12.041673 120-129
TCTGC 1750470 2.1857433 12.499061 210-219
AGTAT 1123220 2.180501 14.896394 220-229
GTTCA 1397715 2.1761386 15.093609 140-149
TTTGC 1664675 2.1735866 13.176004 150-159
AGGGA 1440575 2.164167 6.776881 160-169
TAGTC 1385785 2.1575646 28.000443 70-79
ACTCT 1270490 2.1503274 12.314641 200-209
TTGCA 1371095 2.1346934 15.092263 150-159
ATCTC 1253850 2.1221638 7.953957 100-109
CATAG 1135480 2.1079872 8.664245 220-229
CTCGC 1755390 2.0961154 15.531353 100-109
TCGGC 1904555 2.0920386 8.301361 190-199
TCACA 1035615 2.090028 8.350231 140-149
AATAA 665955 2.0895157 8.825886 80-89
TTGGA 1458155 2.0883646 15.579782 170-179
CACTA 1030815 2.080341 14.862777 240-247
AACCA 846565 2.0372047 13.797677 80-89
ACACG 1145140 2.0330317 14.132205 120-129
GATAT 1044825 2.0283132 7.022015 180-189
CCTTC 1493430 2.0271914 13.589026 200-209
CCAGA 1141805 2.0271106 31.745052 60-69
ATATG 1041945 2.0227222 7.3217015 180-189
CCATA 987975 1.9938834 26.785645 70-79
GCAGA 1220530 1.9932815 15.740578 150-159
GTCTC 1570550 1.9610844 12.158316 180-189
TGTAG 1366975 1.9577768 6.550523 150-159
TCTCG 1563865 1.9527369 15.621219 100-109
GAGTC 1425140 1.9518986 12.870002 210-219
AATAT 735140 1.9344181 10.526705 110-119
GAACT 1039965 1.9306663 8.138898 160-169
CTGTT 1478570 1.9305867 13.435942 140-149
TGCAG 1404620 1.9237938 14.15072 150-159
ATTGT 1179000 1.9194851 8.889594 50-59
CAGAG 1165230 1.9029695 7.192212 140-149
ACCGT 1267380 1.8870001 24.1518 45-49
ACGGC 1440570 1.8868226 13.509938 120-129
CAAAA 617710 1.8534565 23.193735 70-79
ATATA 704215 1.8530433 9.828925 240-247
GTCAA 983400 1.8256547 36.47246 70-79
AGTCT 1172560 1.8255894 13.560015 210-219
CTCCA 1122595 1.8169937 8.630856 150-159
CTGTG 1548865 1.7790686 5.818942 210-219
ACCAT 880045 1.7760644 13.186703 45-49
ATAGT 912500 1.7714313 8.512019 220-229
CACCA 915315 1.7665317 10.89783 230-239
ATGTA 908050 1.7627925 8.072268 160-169
GACGT 1270735 1.7404224 10.964186 60-69
TGTGC 1509595 1.733962 8.37329 220-229
ATACC 855245 1.7260141 19.67612 70-79
TCTGG 1494930 1.7171175 11.835821 180-189
GTCTG 1489105 1.7104267 9.531269 210-219
TCAGA 920605 1.7090776 10.388966 130-139
TGCTC 1362930 1.7018373 6.521127 150-159
GCTCT 1355715 1.6928284 10.942719 130-139
GTGCT 1456895 1.6734296 7.2620816 220-229
GTCCC 1392500 1.6627876 23.917507 50-59
AGATC 881810 1.6370559 8.196674 130-139
GCGTA 1161190 1.5903875 10.817203 220-229
AATTG 814465 1.5811166 10.862698 170-179
ATCGG 1148725 1.5733153 9.405227 190-199
GTTGT 1302060 1.563915 5.2499456 210-219
CAGAC 880400 1.5630237 19.544914 60-69
GCTCC 1308600 1.5626024 7.444856 150-159
GAACC 872475 1.548954 11.349657 70-79
TGACT 994495 1.5483552 10.458428 190-199
GGAAT 900610 1.5380126 10.391452 170-179
CAAAG 692500 1.5329528 26.372347 70-79
TTCAG 983310 1.5309407 7.7701344 150-159
TAGTT 936450 1.5245986 9.724818 220-229
GAATT 784105 1.5221789 11.932209 170-179
TGGTT 1260620 1.5141411 20.453001 10-14
CCTCA 927745 1.5016162 9.561344 130-139
AGCCG 1146210 1.5012773 10.913449 120-129
CCATC 919635 1.4884897 13.761041 240-247
ACTTC 875865 1.4824175 7.5039845 220-229
CCACC 956150 1.4799716 8.264673 230-239
GTCAC 991075 1.4756098 6.2611756 140-149
ACGGA 900565 1.4707377 10.153985 200-209
GCTCA 987495 1.4702797 10.189512 140-149
TGATA 749820 1.4556215 6.564573 180-189
ATCAT 688930 1.4538912 6.61714 240-247
GTAGG 1152795 1.4524004 6.416733 160-169
CCTCG 1202440 1.4358363 8.808654 130-139
AACTG 771625 1.4325005 7.77642 160-169
CTGGT 1243195 1.4279678 5.348871 160-169
CCTTG 1143045 1.4272755 11.83666 20-24
GTGTT 1185395 1.4237877 8.087067 160-169
CTGCT 1137025 1.4197586 5.889185 140-149
AGAAC 639210 1.4149873 8.724168 160-169
GGCCG 1452735 1.4037647 10.866552 120-129
ATAAC 552655 1.3906934 8.886763 80-89
TTGGC 1206470 1.3857844 10.935631 60-69
TCCCA 849830 1.3755057 9.647945 240-247
GGACG 1140485 1.3741082 5.2116017 170-179
GAGAA 673600 1.3716576 8.362488 160-169
ACTGA 736625 1.3675239 6.6672792 180-189
GATCT 871515 1.3568845 6.5964723 130-139
GGTCA 971060 1.3299819 5.071323 130-139
TTGTA 811940 1.3218886 6.616994 220-229
GTCCA 883135 1.3148983 20.95566 70-79
CATAC 647310 1.3063698 24.726934 70-79
AATAG 564025 1.3056004 8.303035 240-247
CCCAC 840095 1.3003366 9.854117 240-247
CACTG 867505 1.2916268 5.1791415 230-239
TCACG 862315 1.2838993 9.9282255 200-209
CAGCG 968320 1.2682815 8.192245 160-169
GTGGT 1197365 1.2651453 14.501796 50-59
GGAGT 997530 1.256783 7.9531116 210-219
GGCTC 1133330 1.2448946 8.057119 130-139
CTGAT 794100 1.2363548 6.6465364 180-189
ATAGA 533475 1.2348835 8.466701 240-247
ATATC 584795 1.2341287 8.51726 70-79
TGGTC 1074235 1.2338957 15.856671 50-59
TATGG 859175 1.2305075 5.476145 190-199
AAAAG 444400 1.2266101 17.0897 70-79
TATAC 581200 1.2265419 5.1497097 110-119
TTCCA 724530 1.22628 9.060773 230-239
GCATA 658130 1.2218001 8.038983 220-229
TCTGT 930060 1.2143908 7.0211096 210-219
TAACT 574730 1.212888 6.2551866 240-247
TCCAC 749240 1.2126942 8.267169 240-247
AAATA 383890 1.204502 5.2931285 9
TGCCC 994510 1.1875467 7.444119 60-69
GAGGG 1059080 1.1738019 5.893139 200-209
TGGAC 844965 1.1572798 8.056586 170-179
AGCGT 844545 1.1567045 8.059618 160-169
CTACC 713160 1.1542963 8.578319 230-239
AGGTT 800945 1.1471108 26.938244 10-14
AGCTT 735825 1.1456251 5.408004 150-159
GAAGA 561885 1.1441712 20.053574 35-39
CTCTT 803135 1.1399909 6.28813 200-209
CGGCT 1034620 1.1364676 7.1856685 130-139
TTGGT 945115 1.1351854 12.835413 25-29
CTTGA 728525 1.1342595 6.370976 200-209
CTCGG 1020170 1.1205952 8.160082 130-139
TGCGT 975400 1.1203712 8.441078 210-219
GCTGA 805310 1.1029676 10.904816 30-34
AAGTA 473510 1.0960771 15.024049 70-79
CATGT 703305 1.094994 7.0187144 150-159
CTGCC 914615 1.0921438 6.433454 200-209
CGTGG 1074660 1.0858803 10.07299 50-59
AACTT 514525 1.0858337 6.637391 210-219
TATCA 514455 1.0856857 8.304327 70-79
ATAAA 345180 1.0830448 5.0528846 7
ACTAT 511970 1.0804417 5.776808 230-239
AGAGC 661510 1.0803304 6.0129395 140-149
CTTCT 760990 1.0801691 6.2604685 210-219
AAAGT 466585 1.0800471 18.690083 70-79
TATAA 409260 1.0769104 9.520603 240-247
CGGAG 889400 1.0715896 7.715725 210-219
CGAAC 601410 1.067717 11.646307 70-79
ACGTC 715810 1.0657682 14.508033 70-79
TAGGC 776805 1.0639266 7.366121 160-169
GTGGA 841085 1.0596788 11.818366 25-29
CCATG 706850 1.0524278 7.092849 150-159
AAGAG 516720 1.0522014 15.309138 35-39
CAATA 417695 1.0510819 6.5569243 100-109
CCGTA 705865 1.0509611 10.194476 70-79
AAAAT 334250 1.0487504 5.88909 90-99
CATCA 518980 1.0473803 8.021393 230-239
ACGTG 761845 1.0434371 6.6152945 170-179
TTGAG 721860 1.0338454 6.248125 200-209
AGTTC 663090 1.0323821 5.147852 160-169
TATGT 630390 1.026314 6.839786 220-229
TCCCC 786685 1.0211928 5.9046364 90-99
CTGCG 928995 1.0204449 6.81359 210-219
CGACT 682505 1.0161806 6.2562613 200-209
AGAGA 497425 1.0129108 14.294163 35-39
ATCCA 499495 1.0080566 13.733384 240-247
CTGAA 540290 1.0030333 15.46113 35-39
TGCTA 641670 0.9990326 5.043892 230-239
TCGAC 668235 0.9949338 6.3904085 190-199
TCATA 469440 0.99068797 5.3517675 240-247
TATTT 532155 0.98486656 6.872546 220-229
AGCTG 711695 0.97475076 5.942761 90-99
TGTAA 500870 0.9723362 7.0107837 230-239
AGTTT 595545 0.9695842 5.6079974 220-229
ACTCG 650345 0.96829754 6.0715604 190-199
ATGAT 497855 0.9664832 10.675591 240-247
GAGCT 703980 0.96418417 5.3182755 140-149
TTCTG 738315 0.9640269 5.881322 210-219
ACTGG 701855 0.9612738 5.4288974 160-169
CTTCC 706175 0.95856637 6.948584 230-239
CTCGA 639280 0.951823 5.772203 190-199
TAGAT 489090 0.9494677 6.5635314 240-247
GACCG 724040 0.94832957 22.133587 45-49
ACAAT 375870 0.9458341 7.7552514 100-109
TCTAC 556840 0.94246167 5.966839 180-189
ACTAA 373195 0.93910277 12.300572 240-247
GTATT 576020 0.9377963 6.446482 220-229
GGGGT 1004020 0.9332282 8.9376 10-14
AGGGG 840710 0.9317776 5.061945 10-14
CCATT 549180 0.9294972 6.5689697 50-59
TACAA 369245 0.9291629 6.1709485 100-109
TACTG 593795 0.9244949 5.3121147 180-189
CCAGC 648585 0.923483 6.410854 80-89
GTCTA 589740 0.9181815 5.8933764 180-189
ACCCA 470455 0.90796465 11.428196 240-247
TGCAT 581565 0.90545374 6.189112 210-219
GGAGG 812525 0.9005395 8.893649 30-34
ATCAA 355525 0.8946381 14.625741 70-79
CGTCC 743405 0.8877017 11.996654 70-79
CCCCC 710715 0.8822657 5.2383823 80-89
TATGA 453945 0.8812409 8.595137 240-247
AAAGA 314730 0.8687016 5.893706 8
TACCG 573325 0.85362256 13.412497 70-79
TGAAG 498395 0.85113174 10.230936 35-39
GGTCC 758725 0.83341366 15.611093 50-59
TTTAT 449490 0.8318774 5.8803053 220-229
ATTTA 369305 0.8149762 5.675333 220-229
TGAGA 473450 0.80853206 5.3773913 240-247
AGCCA 436250 0.7744992 6.858907 80-89
CCCAA 400980 0.7738799 7.3153925 240-247
GCGTG 746340 0.7541324 5.6209383 160-169
ATGGG 595375 0.7501099 17.52154 15-19
TATTA 339410 0.7490044 5.153606 230-239
TCGAA 401980 0.74626464 14.656159 70-79
AAAGG 364205 0.7416338 9.511502 10-14
CATTG 475950 0.741019 6.1380825 50-59
GTAGC 538380 0.7373753 5.3115096 80-89
AAAGC 332565 0.7361826 11.351643 70-79
CTAAT 343675 0.7252784 9.294815 240-247
CCGAA 406590 0.72184205 6.047449 90-99
AAGGT 421835 0.7203868 11.392595 10-14
CATAT 339630 0.71674204 6.3047066 240-247
TAACC 351900 0.71018755 5.957544 80-89
AGCAG 422080 0.68931055 5.124905 80-89
TAGGG 540050 0.6804062 5.2258096 10-14
CCCAT 407825 0.6600916 5.321462 240-247
AGAAA 238630 0.6586543 5.273316 7
CCAAT 325915 0.6577459 7.360509 240-247
ATCCC 406245 0.6575342 5.1483026 240-247
ATAGG 384590 0.6567818 5.548454 10-14
TAGGT 447975 0.6415882 6.3115664 10-14
CCCGA 449625 0.64019537 5.05388 90-99
AATCC 306035 0.61762506 6.8800898 240-247
GGTGG 661420 0.61478436 12.051412 25-29
AATGG 355300 0.60676193 7.0969086 10-14
GAGGC 492880 0.5938443 6.9601 30-34
CATCC 360465 0.5834363 7.822402 240-247
GAGGT 459735 0.5792178 8.188648 10-14
AAGCA 259270 0.5739331 7.471532 70-79
GAAGG 381535 0.5731776 6.149601 10-14
GAAAA 206955 0.57122654 6.387973 8
GTCGA 408455 0.5594276 9.943562 70-79
CAATC 275575 0.55615216 6.8548536 240-247
AAGGG 358785 0.53900045 6.9792943 10-14
GGGTC 529685 0.5352153 5.013222 70-79
TGATC 335515 0.522372 6.1429505 240-247
GGGCC 525825 0.5080999 9.072107 15-19
ATAAG 219195 0.5073908 5.863253 9
AGAGG 329525 0.4950433 5.4119306 10-14
TGGGC 475815 0.48078287 9.881691 15-19
AAGAA 173770 0.479631 5.9959683 9
CGGTT 410300 0.47128186 9.397798 10-14
TAAAA 146980 0.46116784 5.820117 8
TTGCC 367350 0.45869556 5.369814 60-69
GATCC 305375 0.45467234 6.1108313 240-247
TAAGG 262705 0.44863325 6.3908033 10-14
AGAAG 217920 0.44375238 5.2534313 9
GGTCG 407055 0.41130498 5.03219 70-79
TGGGT 377215 0.39856833 5.6321783 10-14
CGATG 275590 0.3774532 7.2911897 15-19
TAAAG 161400 0.3736074 5.6688595 9