FastQCFastQC Report
Thu 4 May 2017
HCGCLBCXY_s2_1_illumina12index_1_SL248356.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s2_1_illumina12index_1_SL248356.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2070648
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 2478735 8.6517105 58.066647 110-119
ATACA 2492860 8.33231 37.91103 110-119
CACAG 3222415 7.5948625 43.558876 110-119
CAGTA 2899355 7.135826 42.39729 60-69
TCCCT 3949315 7.1148944 117.25695 30-34
AGTAA 2232245 6.8489885 47.8851 110-119
GGAGA 3224875 6.404504 101.36473 35-39
GTAAT 2445115 6.284149 39.40918 110-119
GGTGA 3740000 6.2216682 109.26827 45-49
CCCTG 3819375 6.048555 80.06313 30-34
AATAC 1745825 5.835367 41.13203 110-119
GTAGT 2955330 5.5927896 33.316574 80-89
ACAGT 2246375 5.5287266 35.688168 110-119
AGACG 2524825 5.4624414 105.29026 40-44
CTCAG 2616605 5.1658196 37.767616 140-149
GTGAC 2816110 5.103496 88.5536 45-49
ACTCC 2323875 4.998006 131.98354 30-34
TAGTA 1911395 4.9124446 41.738274 80-89
GAGAC 2221610 4.806438 147.21634 40-44
CCCCA 2278975 4.6937413 99.47435 60-69
CACTC 2117410 4.5539575 108.86424 25-29
GCACA 1902850 4.4847994 34.663727 110-119
ATGCA 1803330 4.4383144 108.93198 25-29
ACCAG 1882020 4.435705 90.62517 45-49
AGTAG 1915645 4.3278775 31.950672 80-89
CTCCC 2482850 4.2834406 76.29881 30-34
CGTGT 2821455 4.2830577 21.202433 120-129
GGCCC 3069455 4.2730045 60.405777 60-69
TGACC 2147115 4.238931 145.75577 45-49
AGATA 1347545 4.1345463 21.161476 150-159
GTGTC 2720120 4.129228 21.9992 120-129
CCCAG 2115615 3.9997544 88.905785 60-69
TACAC 1486155 3.9846468 28.091677 110-119
ATGGT 2072650 3.9223697 26.15198 190-199
GACCA 1644990 3.8770523 104.26652 45-49
AGGAG 1943785 3.860298 102.429184 35-39
TGAGG 2313940 3.849349 73.97477 35-39
TGCAC 1943560 3.8370638 130.53676 25-29
GACGG 2383745 3.7974377 115.05977 40-44
CAGAT 1524165 3.7512395 20.051378 150-159
ACGGT 2060610 3.7343407 100.75213 40-44
CTGAG 2059865 3.732991 109.66605 35-39
GAGGA 1874405 3.7225113 132.99449 35-39
CCTGA 1877250 3.7061512 97.8171 30-34
GGATG 2226160 3.703323 83.746994 20-24
TGTCC 2194975 3.6298885 19.72908 120-129
GCACT 1817935 3.589049 101.30953 25-29
AGGCT 1934675 3.506115 21.426905 140-149
CAGGG 2192365 3.492559 37.076218 50-59
CGGAT 1905235 3.4527626 92.65693 20-24
GGGTT 2470950 3.4431965 42.759052 50-59
CCCTT 1907320 3.4361348 20.749973 50-59
CCACT 1583865 3.406451 25.321575 240-247
CTCTC 1883225 3.3927264 13.432538 100-109
GCCCC 2224085 3.3729224 73.230515 60-69
CCGTG 2313440 3.3630602 21.722815 120-129
CTTCA 1495165 3.3579755 16.499733 200-209
TCAGG 1850740 3.3540041 22.59072 140-149
GATGC 1824800 3.306994 110.87103 25-29
TCTCA 1453090 3.2634797 22.052925 130-139
TGGTG 2331925 3.2494695 18.46119 190-199
GGTTG 2307920 3.2160194 85.75594 15-19
CTTGG 2107620 3.1994338 14.87068 170-179
TCTTG 1845350 3.1867418 17.549782 170-179
TCGCA 1584015 3.1272342 22.586891 110-119
GTTGG 2218595 3.0915468 62.056522 15-19
CGCAC 1634660 3.0904672 23.01364 100-109
ACAGC 1308345 3.083619 13.457991 120-129
CCAGG 1768500 3.0691552 40.059067 50-59
CCTGG 2101585 3.055085 27.739231 50-59
AAAAA 608625 3.0275853 16.178003 130-139
AGGGT 1812460 3.0151136 52.645424 50-59
TCCAT 1325610 2.9771736 22.348871 70-79
GCGGA 1862910 2.967719 105.26607 20-24
TGTCT 1682355 2.9052646 19.132238 180-189
TCTCT 1541470 2.8999205 14.788223 180-189
TGGCC 1985490 2.8863177 44.502705 60-69
AGATG 1273150 2.8763351 18.671915 180-189
CGGTG 2141835 2.8581138 72.92396 40-44
ACCAC 1106305 2.8405094 16.507858 230-239
AGTCA 1137855 2.8004625 30.874788 70-79
GCCGT 1906560 2.7715764 20.472383 120-129
GGCTG 2066965 2.7582057 14.044844 140-149
GATGG 1654005 2.7515159 14.797344 190-199
CGGCC 1969840 2.7422247 11.195099 120-129
CAGCT 1383780 2.7319207 13.213862 150-159
TTCCC 1515030 2.7294042 45.16834 50-59
TGTTC 1580230 2.7289047 14.339201 150-159
ACTAC 1015375 2.7224016 15.486902 240-247
GAGAT 1201220 2.713829 17.995863 180-189
CCAGT 1373035 2.7107077 38.45445 60-69
GCCCT 1708900 2.7063003 16.42142 200-209
GGTTC 1773555 2.6923125 46.833202 50-59
TTGGG 1927740 2.686249 93.19372 15-19
TCAAA 797565 2.665837 53.97681 70-79
TCCTC 1479615 2.6656022 17.812899 130-139
GGCGG 2270715 2.663599 52.114822 20-24
TTCTT 1344310 2.6409175 19.773588 170-179
TACCA 971955 2.6059852 17.484692 230-239
CACGG 1480500 2.5693438 10.339524 120-129
CTGGA 1403745 2.5439372 14.320207 180-189
TGGGG 2021770 2.476523 64.089294 15-19
TCATT 1052825 2.4691584 18.393696 150-159
GATAC 998300 2.4569929 16.141954 150-159
TACAG 996930 2.4536214 16.578733 160-169
GCTGT 1615350 2.4521523 11.189793 140-149
GTGAA 1083265 2.447342 18.523407 190-199
CATTT 1041255 2.4420233 18.476242 150-159
TCAGC 1228855 2.426061 14.747467 140-149
TGAAT 943685 2.425349 16.16371 190-199
ACACA 757715 2.42532 16.128159 120-129
GTTCC 1461385 2.416731 51.283436 50-59
GACTC 1222865 2.414235 15.874502 190-199
ATTTG 1119740 2.4106107 16.715857 150-159
ACAGA 816705 2.3996365 9.967098 140-149
TTGTC 1385840 2.3932118 14.337718 170-179
TTCAT 1016755 2.3845642 16.91278 140-149
CGTAG 1310350 2.3746822 13.359 80-89
TGGAG 1426940 2.3737826 14.189056 180-189
TTCAC 1053875 2.3668866 18.287392 200-209
CAGGC 1360295 2.3607328 18.093845 140-149
AATCG 956785 2.3548172 15.9803095 190-199
GTTCT 1349475 2.330413 16.632065 160-169
TACTA 831645 2.3284566 13.269793 240-247
GAATC 945770 2.3277073 16.458132 190-199
TGGAA 1018005 2.2999046 10.946728 170-179
CTACT 1015730 2.2812176 9.55286 230-239
CTCTG 1352950 2.2374094 14.659173 180-189
GGGGC 1905525 2.235223 59.32569 15-19
TCACA 832125 2.231076 8.413795 140-149
AGCTC 1129010 2.2289424 13.292372 140-149
GTCCT 1325780 2.1924777 15.764925 130-139
CTGGC 1508010 2.1922023 33.088345 60-69
CAGCC 1156730 2.186899 11.917285 120-129
TCTGC 1317420 2.1786525 12.449273 210-219
GGGCG 1848460 2.1682844 72.29065 20-24
ACTCT 964015 2.165071 12.368004 200-209
TTTGC 1252045 2.1621609 12.989416 150-159
GTTCA 1046885 2.1582632 15.043337 140-149
AGGGA 1086400 2.1575572 6.836248 160-169
AGTAT 838425 2.154822 14.668652 220-229
TAGTC 1042375 2.1489656 27.953085 70-79
AATAA 513920 2.1414344 8.952517 80-89
ATCTC 951625 2.1372445 7.871864 100-109
TTGCA 1030410 2.1242983 14.994198 150-159
CTCGC 1331210 2.1081715 15.5432005 100-109
CATAG 850415 2.0930216 8.66386 210-219
TTGGA 1097875 2.0776644 15.387949 170-179
TCGGC 1424990 2.0715158 8.212264 190-199
CACTA 771915 2.0696418 14.603617 240-247
AACCA 644810 2.0639296 13.896701 80-89
ACACG 869165 2.0485222 14.239518 120-129
ATATG 791420 2.0340154 7.1793833 180-189
GATAT 787010 2.0226812 6.8914967 180-189
CCTTC 1119920 2.0175931 13.543052 200-209
CCATA 748985 2.0081625 26.81785 70-79
GCAGA 917430 1.9848535 15.644395 150-159
TGTAG 1042170 1.9722458 6.576467 150-159
TCTCG 1186910 1.9628247 15.636258 100-109
GTCTC 1181775 1.9543327 11.950608 180-189
AATAT 557720 1.946651 10.676818 110-119
CTGTT 1117485 1.9297887 13.345576 140-149
GAGTC 1064135 1.9284787 12.724088 210-219
GAACT 783085 1.9273107 8.036478 160-169
TGCAG 1057495 1.9164456 14.082131 150-159
CAGAG 884435 1.9134691 7.0462832 140-149
ATTGT 888665 1.9131452 8.911738 50-59
ACGGC 1098515 1.906425 13.495262 120-129
CCAGA 805405 1.8982472 31.075977 60-69
ACCGT 954365 1.8841504 24.294334 45-49
CAAAA 463955 1.8513167 23.110117 70-79
ATATA 526190 1.8365996 9.764174 240-247
GTCAA 740435 1.8223417 36.443184 70-79
AGTCT 875570 1.8050795 13.387387 210-219
ACCAT 671720 1.8010013 13.403793 45-49
CTCCA 836090 1.7981962 8.680954 150-159
CTGTG 1180885 1.7926208 5.859927 210-219
ATAGT 690615 1.7749382 8.508783 220-229
ATGTA 684990 1.7604815 8.086751 160-169
GACGT 965870 1.7503979 10.990865 60-69
TGTGC 1147830 1.742442 8.330006 220-229
TCAGA 700010 1.7228485 10.633441 130-139
TGCTC 1038550 1.7174777 6.629188 150-159
ATACC 638750 1.7126029 19.977547 70-79
TCTGG 1120925 1.7015996 11.729106 180-189
GTCTG 1113460 1.6902674 9.39002 210-219
GCTCT 1017775 1.6831217 10.840671 130-139
GTGCT 1099785 1.6695083 7.1269083 220-229
GTCCC 1049590 1.6621839 23.931362 50-59
CACCA 637440 1.6366683 10.727666 230-239
AATTG 612835 1.5750369 10.836406 170-179
GCTCC 993860 1.5739269 7.374674 150-159
GCGTA 867640 1.5723809 10.74889 210-219
GAACC 664090 1.565184 11.705447 70-79
GTTGT 987170 1.5648656 5.1941037 220-229
CAGAC 660995 1.5578893 19.714867 60-69
ATCGG 858170 1.5552188 9.326379 190-199
TGACT 752515 1.5513886 10.406707 190-199
TTCAG 743955 1.5337412 7.7499213 150-159
CAAAG 521200 1.5313859 26.358673 70-79
AGATC 615830 1.5156665 8.16657 130-139
TGGTT 955970 1.5154074 20.231287 10-14
GGAAT 669160 1.5117846 10.242224 170-179
TAGTT 701690 1.5106198 9.693198 220-229
GAATT 587085 1.5088573 11.856534 170-179
CCTCA 699890 1.5052677 9.413773 130-139
AGCCG 862715 1.4972043 10.857979 120-129
CCATC 695675 1.4962025 13.74633 240-247
CCACC 726365 1.4960102 8.2880535 230-239
ACTTC 663340 1.4897882 7.7139635 220-229
GCTCA 752675 1.4859651 10.17884 150-159
GTCAC 746580 1.4739319 6.339709 140-149
GATCT 709355 1.4624097 6.5983434 130-139
GTAGG 878340 1.4611603 6.3977795 160-169
TGATA 567925 1.4596145 6.4407816 180-189
ACGGA 674425 1.4591138 10.1708 200-209
CCTTG 875960 1.4485981 12.31196 20-24
CTGGT 948505 1.4398606 5.3340297 170-179
CCTCG 904910 1.4330611 8.804155 130-139
AACTG 579765 1.4269043 7.742254 160-169
GTGTT 897590 1.4228632 8.078459 160-169
AGAAC 484105 1.4223934 8.663334 160-169
CTGCT 857075 1.4173678 5.905184 140-149
GGCCG 1101900 1.4080929 10.896396 120-129
ATAAC 417025 1.3938935 8.897685 80-89
TTGGC 915860 1.3903042 11.081994 60-69
TCCCA 645655 1.3886237 9.97557 240-247
GGACG 865885 1.3794028 5.188624 170-179
TCACG 698335 1.3786846 10.048925 200-209
GAGAA 511170 1.3786774 8.335191 160-169
ACTGA 554410 1.3645011 6.640202 180-189
GGTCA 739245 1.339697 5.0012603 130-139
GTCCA 675890 1.3343726 20.923576 70-79
TTGTA 617165 1.3286515 6.7423773 220-229
CATAC 489690 1.3129463 24.788406 70-79
ATCAT 465990 1.3046882 6.594483 240-247
CACTG 659275 1.3015704 5.26814 230-239
AATAG 423780 1.3002446 8.149308 240-247
CCCAC 629700 1.2969204 10.107683 240-247
GTGGT 910275 1.2684417 14.393388 50-59
CTGAT 603430 1.2440344 6.601985 180-189
CAGCG 716355 1.2432032 7.9892235 160-169
GGAGT 746925 1.2425454 7.724636 210-219
TATGG 655000 1.2395494 5.5449815 190-199
TGGTC 816290 1.239154 15.772421 50-59
GGCTC 851055 1.2371833 7.980843 130-139
ATAGA 402995 1.236472 8.520658 240-247
ATATC 441285 1.2355187 8.617711 70-79
AAAAG 333640 1.2220812 17.129938 70-79
TTCCA 543130 1.2198099 9.067727 230-239
TAACT 435235 1.2185798 6.1717653 240-247
GCATA 494810 1.217815 8.118438 220-229
TCTGT 702530 1.2132016 6.9415607 210-219
TCCAC 560160 1.2047477 8.363276 240-247
TATAC 429850 1.2035028 5.0600486 110-119
AAATA 288045 1.200244 5.336413 9
TGCCC 751395 1.1899471 7.291553 60-69
CATCA 440415 1.1808312 8.222792 230-239
GAGGG 796405 1.1646137 5.7044835 200-209
TGGAC 642380 1.1641533 8.116032 170-179
TTGGT 732370 1.1609557 13.337433 25-29
CTCTT 616695 1.1601695 6.3087387 200-209
AGGTT 607080 1.1488634 27.26643 10-14
AGCTT 555380 1.1449741 5.3836226 150-159
AGCGT 630290 1.142243 7.8895288 160-169
CTACC 528935 1.1375915 8.480284 230-239
CTTGA 551590 1.1371609 6.340863 200-209
GAAGA 420115 1.1330928 20.070541 35-39
CGGCT 773905 1.1250299 7.1866007 130-139
GCTGA 619490 1.1226709 11.3798485 30-34
CTCGG 763725 1.1102313 8.079945 130-139
ATAAA 265585 1.1066564 5.5371084 7
TGCGT 728655 1.1061213 8.359058 210-219
CATGT 536345 1.1057315 7.06758 150-159
AACTT 394480 1.1044731 6.7068777 210-219
AAGTA 357940 1.0982338 15.198431 70-79
CGAAC 463855 1.093253 11.978764 70-79
CTGCC 690050 1.0927981 6.468938 200-209
ACTAT 389895 1.0916358 5.8175645 230-239
TATCA 389140 1.0895222 8.383969 70-79
TATAA 310700 1.084459 9.530321 240-247
GTGGA 650695 1.0824621 12.297149 25-29
CGTGG 810055 1.080956 10.000507 50-59
CTTCT 573000 1.0779674 6.210986 210-219
AGAGC 496635 1.0744665 5.8957715 140-149
CAATA 319155 1.066766 6.60505 100-109
TAGGC 587815 1.0652679 7.339852 160-169
CCATG 539330 1.0647696 7.123578 150-159
AAAAT 255330 1.0639251 5.9158545 90-99
AAAGT 346435 1.062934 18.7273 70-79
ACGTG 585890 1.0617794 6.583155 170-179
CGGAG 666300 1.0614529 7.526768 210-219
ACGTC 534490 1.0552143 14.477806 70-79
AAGAG 388165 1.0469203 15.342026 35-39
CCGTA 530010 1.0463697 10.250033 70-79
TTGAG 546420 1.034068 6.156424 200-209
AGAGA 382575 1.0318437 14.377404 35-39
AGTTC 496710 1.0240198 5.1292725 160-169
TCCCC 592890 1.0228604 6.0398507 90-99
CGACT 517890 1.0224417 6.3303785 200-209
TATGT 471570 1.0152104 6.6745768 220-229
CTGCG 696815 1.0129639 6.6834526 210-219
TCGAC 507885 1.0026895 6.2803607 190-199
CTGAA 404865 0.99644446 15.907608 35-39
ATCCA 370880 0.9943956 13.882584 240-247
TATTT 399150 0.97753716 6.799574 220-229
AGTTT 452225 0.97356373 5.574865 220-229
TGTAA 377000 0.96892136 6.886978 230-239
ACTGG 533160 0.96621925 5.3929276 160-169
ACTCG 488715 0.96484315 5.9565654 190-199
ACAAT 288450 0.9641354 7.9061637 100-109
TCATA 343915 0.9629002 5.2813783 240-247
AGCTG 530890 0.96210545 5.8653903 90-99
CTCGA 486855 0.9611712 5.652764 190-199
TTCTG 556285 0.9606506 5.804149 210-219
ATGAT 373455 0.9598105 10.616524 240-247
GAGCT 529010 0.9586984 5.1832175 140-149
CTTCC 528040 0.95129126 6.889911 230-239
TCTAC 422250 0.9483269 5.970327 180-189
TACAA 281900 0.94224226 6.2620215 100-109
GACCG 542395 0.94130296 22.301924 45-49
TAGAT 365795 0.9401237 6.5973377 240-247
CCATT 414340 0.9305618 6.726628 50-59
GGGGT 759440 0.9302593 8.781613 10-14
GTCTA 449595 0.9268873 5.896466 180-189
GGAGG 633160 0.92589426 9.311558 30-34
GTATT 430015 0.92574936 6.397521 220-229
ACTAA 276725 0.924945 12.11566 240-247
TACTG 448195 0.92400104 5.2953362 180-189
CCAGC 480150 0.9077654 6.398411 80-89
TGCAT 437025 0.90097296 6.252574 210-219
ATCAA 267025 0.892523 14.583039 70-79
ACCCA 346535 0.88975096 11.296598 240-247
CGTCC 561555 0.8893069 11.942994 70-79
TATGA 341850 0.87858313 8.564875 240-247
CCCCC 529300 0.8744595 5.2242517 80-89
AAAGA 237010 0.8681377 5.211178 8
TACCG 429555 0.84804696 13.568861 70-79
TGAAG 374540 0.84617096 10.55722 35-39
GGTCC 576840 0.83855546 15.5354805 50-59
TTTAT 337950 0.8276554 5.883385 220-229
TGAGA 357300 0.80722183 5.237855 240-247
ATTTA 275525 0.8055556 5.6359873 220-229
CCCAA 305330 0.7839544 7.3503203 240-247
GTACC 392740 0.775365 5.0389495 80-89
AGCCA 326085 0.76854485 7.057905 80-89
TCGAA 311060 0.76557374 15.210648 70-79
ATGGG 458640 0.7629695 18.182753 15-19
TATTA 255480 0.7469498 5.2578425 230-239
CATTG 360555 0.7433219 6.257936 50-59
GTAGC 409970 0.74296814 5.4153085 80-89
GCGTG 556155 0.742146 5.4888005 160-169
AAAGG 275040 0.74181074 9.55629 10-14
AAAGC 251385 0.7386174 11.422175 70-79
CCGAA 313010 0.73772866 6.240286 90-99
AAGGT 318025 0.7184908 11.82114 10-14
CTAAT 256615 0.7184759 9.216371 240-247
CATAT 254795 0.71338016 6.25432 240-247
TAACC 261520 0.7011818 5.824094 80-89
AGCAG 316705 0.68518907 5.16047 80-89
TAGGG 406465 0.67617387 5.0831337 10-14
ATCCC 310850 0.66855156 5.2535677 240-247
CCAAT 248295 0.6657232 7.4665446 240-247
TAGGT 348750 0.6599891 6.325693 10-14
ATAGG 291820 0.6592877 5.5366955 10-14
CCCGA 345145 0.6525267 5.184758 90-99
CCCAT 302050 0.6496251 5.256555 240-247
GGTGG 518910 0.63562745 12.545076 25-29
AATCC 228775 0.61338663 6.880032 240-247
GAGGC 383500 0.61093676 7.3871884 30-34
AATGG 265855 0.6006268 6.9959226 10-14
GAGGT 355560 0.591491 8.1512575 10-14
CATCC 274675 0.5907492 7.9004383 240-247
AAGCA 197875 0.58139473 7.6255484 70-79
GAAGG 286580 0.5691392 6.1571116 10-14
GAAAA 153500 0.5622511 5.600207 8
GTCGA 309840 0.56150764 10.128973 70-79
CAATC 207420 0.55613005 6.973991 240-247
GGGTC 410300 0.54751366 5.1817794 70-79
AAGGG 274275 0.5447018 7.027799 10-14
ATAAG 172865 0.5303855 6.0513325 9
TGATC 254605 0.52489495 6.175268 240-247
GGGCC 408905 0.5225303 9.407086 15-19
TGGGC 372985 0.49771976 10.212604 15-19
AGAGG 244485 0.48553976 5.1912613 10-14
CGGTT 312945 0.4750604 9.393225 10-14
AAGAA 129520 0.4744154 5.6499662 9
TAAGG 206260 0.46598822 6.4306474 10-14
TAAAA 111660 0.46527195 5.701781 8
GATCC 230810 0.4556755 6.1049933 240-247
AAATG 147490 0.45252976 5.293494 9
AGAAG 167265 0.45113063 5.762419 9
TTGCC 271670 0.4492679 5.2138557 60-69
GGTCG 314490 0.41966268 5.1293736 70-79
CGATG 216185 0.39178133 7.7590485 15-19
TGGGT 280900 0.39142597 5.564406 10-14
TAAAG 119260 0.36591431 6.0740676 9