FastQCFastQC Report
Wed 3 May 2017
HCGCLBCXY_s1_1_illumina12index_1_SL248356.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s1_1_illumina12index_1_SL248356.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 1929799
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 2307435 8.622706 57.832073 110-119
ATACA 2325450 8.323303 37.736736 110-119
CACAG 3000800 7.5816426 43.442432 110-119
CAGTA 2704265 7.1334705 42.474766 60-69
TCCCT 3674960 7.120002 117.6547 30-34
AGTAA 2079155 6.8270645 47.696968 110-119
GGAGA 3005125 6.390071 101.04352 35-39
GTAAT 2278030 6.273884 39.367863 110-119
GGTGA 3485280 6.2159944 109.1682 45-49
CCCTG 3555260 6.0524597 80.210815 30-34
AATAC 1627475 5.825095 40.91841 110-119
GTAGT 2754790 5.591501 33.115204 80-89
ACAGT 2092765 5.5204196 35.67015 110-119
AGACG 2350695 5.44855 105.05064 40-44
CTCAG 2436660 5.163581 37.75685 140-149
GTGAC 2620955 5.095351 88.5731 45-49
ACTCC 2163260 4.9969654 131.86218 30-34
TAGTA 1779755 4.9015927 41.43409 80-89
GAGAC 2070590 4.7993097 146.74641 40-44
CCCCA 2125800 4.703213 99.51587 60-69
CACTC 1969775 4.55003 108.963165 25-29
GCACA 1772560 4.478444 34.577343 110-119
ATGCA 1681295 4.4350195 108.6849 25-29
ACCAG 1754330 4.432386 90.514275 45-49
AGTAG 1787515 4.325733 31.729174 80-89
CTCCC 2308845 4.2844677 76.42443 30-34
CGTGT 2626405 4.2825856 21.256468 120-129
GGCCC 2861805 4.280885 60.51043 60-69
TGACC 1997845 4.233679 145.92224 45-49
AGATA 1257175 4.1280303 21.10305 150-159
GTGTC 2530215 4.1257396 22.053707 120-129
CCCAG 1972990 4.0045676 88.93007 60-69
TACAC 1383735 3.9787447 28.104233 110-119
ATGGT 1928315 3.9139738 26.140726 190-199
GACCA 1535285 3.8789594 104.274315 45-49
AGGAG 1812345 3.853754 102.23678 35-39
TGAGG 2154645 3.8428082 73.82193 35-39
TGCAC 1812425 3.840751 130.73553 25-29
GACGG 2221860 3.7954583 115.1633 40-44
CAGAT 1419525 3.7445073 19.936209 150-159
ACGGT 1920365 3.7333467 100.707115 40-44
CTGAG 1918855 3.730411 109.441505 35-39
GAGGA 1748250 3.717463 132.7254 35-39
GGATG 2074965 3.7006989 83.67751 20-24
CCTGA 1745280 3.698462 97.779594 30-34
TGTCC 2045630 3.6359096 19.850552 120-129
GCACT 1694355 3.590546 101.378555 25-29
AGGCT 1802000 3.5032353 21.443455 140-149
CAGGG 2041405 3.487199 37.005764 50-59
CGGAT 1773205 3.4472556 92.5936 20-24
GGGTT 2302840 3.4448206 42.7478 50-59
CCCTT 1775215 3.4393663 20.77985 50-59
CCACT 1472175 3.4006119 25.387484 240-247
CTCTC 1754460 3.399155 13.481597 130-139
GCCCC 2074600 3.3827472 73.31825 60-69
CCGTG 2151200 3.3596947 21.758366 120-129
CTTCA 1392165 3.3574824 16.56231 200-209
TCAGG 1724285 3.3521512 22.576443 140-149
GATGC 1701895 3.308623 110.66931 25-29
TCTCA 1357100 3.2729163 22.227423 130-139
TGGTG 2170610 3.2470174 18.475712 190-199
GGTTG 2148090 3.2133293 85.54436 15-19
CTTGG 1964000 3.2024758 14.961748 170-179
TCTTG 1715500 3.1834795 17.629272 170-179
AAAAA 593405 3.1521406 17.322039 130-139
TCGCA 1472910 3.1212766 22.505276 110-119
ACAGC 1222090 3.0876598 13.45566 120-129
GTTGG 2061730 3.0841439 61.93328 15-19
CGCAC 1519360 3.083837 22.99158 100-109
CCAGG 1648870 3.0702627 39.98792 50-59
CCTGG 1956925 3.0562801 27.75189 50-59
AGGGT 1690320 3.014685 52.545628 50-59
TCCAT 1233490 2.974806 22.255545 70-79
GCGGA 1735615 2.964838 105.280624 20-24
TGTCT 1566020 2.9060874 19.17983 180-189
TCTCT 1436485 2.9057217 14.853868 180-189
TGGCC 1850885 2.8906696 44.531944 60-69
AGATG 1190840 2.8817976 18.622494 180-189
CGGTG 1994140 2.8571503 73.00406 40-44
ACCAC 1032880 2.8445768 16.467697 230-239
AGTCA 1065275 2.8100455 30.874214 70-79
GCCGT 1777955 2.776769 20.501198 120-129
GGCTG 1924100 2.7567987 14.058806 140-149
GATGG 1540440 2.7473738 14.820232 190-199
CGGCC 1835640 2.745877 11.257672 120-129
TGTTC 1475920 2.7388875 14.490441 150-159
TTCCC 1408080 2.7280657 45.190575 50-59
CAGCT 1286590 2.726442 13.150562 150-159
ACTAC 947665 2.724884 15.639445 240-247
CCAGT 1282040 2.7167997 38.56006 60-69
GAGAT 1122145 2.7155578 17.967941 180-189
GCCCT 1591145 2.7087586 16.436615 200-209
GGTTC 1647900 2.6870465 46.795666 50-59
TTGGG 1795745 2.6862562 93.26643 15-19
TCCTC 1381460 2.676491 17.936543 130-139
TCAAA 745530 2.668418 53.918716 70-79
GGCGG 2112055 2.658984 52.062958 20-24
TTCTT 1250160 2.6402428 19.848595 170-179
TACCA 907040 2.608072 17.608398 230-239
CACGG 1378835 2.5674465 10.37732 120-129
CTGGA 1310250 2.5472329 14.392995 180-189
TGGGG 1882365 2.474228 64.10231 15-19
TCATT 982120 2.4729366 18.47962 150-159
TACAG 934740 2.4657123 16.652187 160-169
GCTGT 1507680 2.4584057 11.243358 140-149
GATAC 931170 2.4562953 16.099276 150-159
GTGAA 1013015 2.4514666 18.56958 190-199
CATTT 970150 2.442797 18.583485 150-159
ACACA 710645 2.4362175 16.02805 120-129
TGAAT 881625 2.4280684 16.288815 190-199
TCAGC 1141025 2.4179718 14.681287 140-149
GTTCC 1359280 2.4159887 51.310432 50-59
ATTTG 1044835 2.4135406 16.775465 150-159
GACTC 1135025 2.405257 15.748064 190-199
TTGTC 1291355 2.396387 14.324672 170-179
ACAGA 760880 2.392974 9.900391 140-149
TTCAT 949190 2.3900201 16.952164 140-149
CGTAG 1226885 2.3851647 13.333502 80-89
TGGAG 1331425 2.3745959 14.2235565 180-189
TTCAC 983185 2.371146 18.367022 200-209
CAGGC 1269835 2.3644843 18.074064 140-149
AATCG 891545 2.3517702 16.047739 190-199
GTTCT 1258735 2.3358538 16.711708 160-169
GAATC 883020 2.3292823 16.579092 190-199
TACTA 774690 2.3256617 13.400857 240-247
TGGAA 950265 2.2996132 10.961363 170-179
CTACT 943380 2.2751482 9.5820675 230-239
CTCTG 1261485 2.2421675 14.721984 180-189
GGGGC 1773185 2.2323613 59.272102 15-19
TCACA 775675 2.2303495 8.423034 140-149
AGCTC 1048710 2.2223449 13.296591 140-149
GTCCT 1240450 2.2047799 15.839481 130-139
TCTGC 1234065 2.1934311 12.556953 210-219
CTGGC 1404375 2.1933203 33.140022 60-69
CAGCC 1074965 2.181851 11.909756 120-129
TTTGC 1168270 2.1679764 13.034933 150-159
GGGCG 1721790 2.1676576 72.11511 20-24
GTTCA 978135 2.164115 15.075945 140-149
TAGTC 977100 2.1618247 28.013159 70-79
AGTAT 784625 2.1609223 14.714311 220-229
AGGGA 1012140 2.1522055 6.819899 160-169
ACTCT 892140 2.151573 12.342279 200-209
ATCTC 886570 2.1381397 7.9513445 100-109
TTGCA 961780 2.1279294 15.027039 150-159
AATAA 476615 2.1234987 8.932883 80-89
CTCGC 1235460 2.103242 15.509835 100-109
CATAG 794615 2.0960827 8.696008 210-219
TCGGC 1328505 2.0748281 8.25041 190-199
CACTA 721585 2.0748212 14.770291 240-247
TTGGA 1020525 2.071398 15.375701 170-179
AACCA 598210 2.05077 13.826787 80-89
ACACG 811665 2.0507045 14.331507 120-129
ATATG 736225 2.0276248 7.1186185 180-189
CCTTC 1043425 2.0215697 13.579538 200-209
GATAT 732410 2.017118 6.843438 180-189
CCATA 697995 2.0069914 26.771906 70-79
GCAGA 857515 1.9875879 15.656442 150-159
TGTAG 967005 1.9627669 6.5890875 150-159
TCTCG 1103140 1.9607245 15.597902 100-109
GTCTC 1103040 1.960547 12.033506 180-189
AATAT 519840 1.9426019 10.625514 110-119
GAGTC 996110 1.9365194 12.800012 210-219
CTGTT 1042825 1.9351863 13.420865 140-149
GAACT 728405 1.9214298 7.9869647 160-169
TGCAG 987480 1.9197419 14.061872 150-159
ATTGT 828050 1.9127731 8.896265 50-59
CAGAG 823605 1.9089901 7.008543 140-149
ACGGC 1023680 1.9061337 13.548675 120-129
CCAGA 748080 1.8900542 30.93171 60-69
ACCGT 886870 1.8793862 24.212898 45-49
CAAAA 434425 1.8538462 22.967365 70-79
ATATA 492580 1.8407334 9.566831 240-247
GTCAA 692300 1.82619 36.448082 70-79
AGTCT 820195 1.8146738 13.458917 210-219
ACCAT 625200 1.7976791 13.472419 45-49
CTCCA 775120 1.7904681 8.648007 150-159
CTGTG 1097330 1.7892936 5.8301544 210-219
ATAGT 643265 1.7716051 8.438645 220-229
ATGTA 636060 1.751762 8.093298 160-169
TGTGC 1066490 1.7390064 8.310828 220-229
GACGT 892205 1.7345192 10.935506 60-69
TCAGA 651950 1.7197524 10.575121 130-139
TGCTC 964765 1.7147765 6.6266723 150-159
TCTGG 1045575 1.7049025 11.761711 180-189
GTCTG 1044740 1.7035412 9.457942 210-219
ATACC 590365 1.6975155 19.840036 70-79
GCTCT 951140 1.6905593 10.928275 130-139
GTGCT 1019745 1.6627846 7.1133823 220-229
GTCCC 975400 1.660517 23.915237 50-59
CACCA 593520 1.6345686 10.642182 230-239
GCGTA 812410 1.5793915 10.871469 220-229
AATTG 573435 1.5792875 10.756725 170-179
GCTCC 920985 1.567881 7.336005 150-159
GTTGT 918975 1.5644926 5.175335 220-229
CAGAC 617150 1.5592545 19.618208 60-69
ATCGG 801255 1.5577053 9.359558 190-199
GAACC 615525 1.5551487 11.605554 70-79
TGACT 698585 1.5456129 10.330989 190-199
CAAAG 487980 1.5347011 26.25764 70-79
TTCAG 691750 1.5304904 7.7178993 140-149
GGAAT 627325 1.518108 10.279939 170-179
GAATT 549600 1.513644 11.825765 170-179
TGGTT 888585 1.5127555 20.323084 10-14
AGATC 572530 1.5102534 8.17811 130-139
CCTCA 651630 1.5052156 9.510616 130-139
TAGTT 649725 1.5008471 9.652082 220-229
CCACC 677670 1.4993067 8.275869 230-239
CCATC 648070 1.4969922 13.716845 240-247
AGCCG 803510 1.4961681 10.777907 120-129
ACTTC 617245 1.4886091 7.728824 220-229
GCTCA 698425 1.4800482 10.186981 150-159
GTCAC 696625 1.4762338 6.3307137 140-149
GATCT 662535 1.4658525 6.576821 130-139
TGATA 530595 1.4613026 6.4196143 180-189
ACGGA 629685 1.4595133 10.152971 200-209
GTAGG 818255 1.4593574 6.3575873 160-169
CCTTG 814910 1.4484236 12.391398 20-24
CCTCG 844285 1.4373072 8.816698 130-139
CTGGT 876495 1.4292027 5.291669 170-179
CTGCT 801340 1.4243042 5.879691 140-149
AACTG 539855 1.4240614 7.624054 160-169
GTGTT 835795 1.4228841 8.065968 160-169
AGAAC 449525 1.4137597 8.645114 160-169
GGCCG 1026380 1.4085109 10.930613 120-129
TTGGC 855965 1.3957266 11.0490885 60-69
ATAAC 388040 1.3888816 8.868728 80-89
TCCCA 600150 1.3863007 10.033463 240-247
TCACG 651815 1.3812758 9.973698 200-209
GGACG 802770 1.3713197 5.1020913 170-179
GAGAA 473640 1.3665597 8.301242 160-169
ACTGA 514250 1.3565191 6.548452 180-189
GTCCA 630305 1.3356936 20.889254 70-79
TTGTA 571360 1.319826 6.7298107 220-229
ATCAT 435685 1.3079501 6.6135306 240-247
CATAC 453755 1.3047119 24.656588 70-79
AATAG 396320 1.301347 8.133504 240-247
CCCAC 584865 1.293981 10.102229 240-247
CACTG 610485 1.2936926 5.1886506 230-239
GTGGT 845935 1.265435 14.317988 50-59
GGAGT 699560 1.2476649 7.793018 210-219
CAGCG 669280 1.2462265 7.971328 160-169
GGCTC 795930 1.2430651 8.01302 130-139
ATAGA 376155 1.2351336 8.478716 240-247
TATGG 608325 1.2347403 5.5610456 190-199
CTGAT 557805 1.2341384 6.501621 180-189
ATATC 410870 1.2334542 8.564548 70-79
TGGTC 755985 1.2327005 15.695551 50-59
TTCCA 506655 1.2218992 9.073923 230-239
AAAAG 311100 1.2179159 16.995049 70-79
GCATA 460810 1.215552 8.055164 220-229
AAATA 272685 1.214914 5.1115785 9
TCTGT 654620 1.2147883 6.930521 210-219
TAACT 404120 1.2131903 6.188077 240-247
TCCAC 519680 1.2004212 8.316404 240-247
TATAC 399400 1.1990206 5.179288 110-119
TGCCC 699395 1.1906472 7.226795 60-69
CATCA 413950 1.1902579 8.18238 230-239
GAGGG 743235 1.1647485 5.658586 200-209
TTGGT 680665 1.1587857 13.425798 25-29
TGGAC 594205 1.1551832 7.996676 170-179
CTCTT 570110 1.1532184 6.33133 200-209
AGCGT 589800 1.1466193 7.893738 160-169
CTACC 495635 1.1448791 8.572034 230-239
AGGTT 563830 1.1444271 27.001715 10-14
GAAGA 395070 1.1398672 20.171045 35-39
AGCTT 514135 1.1375189 5.3696876 150-159
CTTGA 513545 1.1362137 6.2708907 200-209
CGGCT 721875 1.1274077 7.2214575 130-139
GCTGA 575800 1.1194022 11.460817 30-34
CTCGG 714695 1.116194 8.116401 130-139
TGCGT 680880 1.1102352 8.366826 210-219
ATAAA 249050 1.1096112 5.70582 7
CATGT 498415 1.1027386 7.0285206 150-159
AACTT 366325 1.0997276 6.6824055 210-219
CTGCC 644540 1.0972621 6.466155 200-209
AAGTA 334005 1.096731 15.198537 70-79
ACTAT 364215 1.0933933 5.8106956 230-239
TATAA 291260 1.0884161 9.526446 240-247
GTGGA 607805 1.0840199 12.390289 25-29
TATCA 360965 1.0836366 8.31652 70-79
CGAAC 428340 1.0822183 11.888867 70-79
CTTCT 532920 1.0779904 6.158245 210-219
CGTGG 752235 1.077782 9.933559 50-59
AGAGC 460925 1.0683533 5.8354907 140-149
AAAGT 323870 1.0634519 18.680656 70-79
CGGAG 622360 1.063137 7.5574 210-219
CCATG 501515 1.0627718 7.1210732 150-159
TAGGC 545010 1.0595441 7.300232 160-169
AAAAT 237450 1.0579288 6.015046 90-99
CAATA 294905 1.0555308 6.5887785 100-109
ACGTC 497720 1.0547297 14.376737 70-79
ACGTG 540510 1.0507957 6.5207987 170-179
AGTGA 433985 1.050231 5.0089526 160-169
CCGTA 493675 1.0461578 10.144452 70-79
AAGAG 362370 1.0455202 15.440595 35-39
AGAGA 358370 1.0339793 14.508472 35-39
TTGAG 508865 1.0328624 6.0888257 200-209
AGTTC 465120 1.0290737 5.2101407 160-169
TATGT 441215 1.0191947 6.748926 220-229
CTGCG 651955 1.0182081 6.751287 210-219
TCCCC 547810 1.0165578 5.9976 90-99
CGACT 479560 1.0162464 6.320322 200-209
ATCCA 346940 0.9975795 13.830999 240-247
TCGAC 470730 0.9975345 6.2600203 190-199
TGCTA 448740 0.99283326 5.014212 230-239
CTGAA 376000 0.9918351 15.843338 35-39
TATTT 371855 0.9775691 6.7868605 220-229
AGTTT 420655 0.9717017 5.5421305 220-229
AGCTG 499295 0.97067034 5.927041 90-99
TCATA 321905 0.9663764 5.253748 240-247
TGTAA 350345 0.96487933 6.85015 230-239
TTCTG 519235 0.9635523 5.7726455 210-219
ATGAT 349450 0.9624143 10.578357 240-247
ACTGG 493960 0.96029866 5.28178 160-169
ACTCG 453050 0.9600685 5.950933 190-199
ACAAT 267355 0.9569231 7.893185 100-109
CTCGA 451485 0.956752 5.635824 190-199
GAGCT 491745 0.9559925 5.120173 140-149
CTTCC 490115 0.9495667 6.838002 230-239
GACCG 505765 0.9417549 22.277649 45-49
TCTAC 389935 0.9404057 5.970927 180-189
TACAA 262285 0.93877643 6.2263207 100-109
TAGAT 340705 0.9383298 6.5625777 240-247
GGGGT 709515 0.9326044 8.889679 10-14
CCATT 385270 0.92915523 6.6995378 50-59
GTATT 401380 0.92717695 6.3326817 220-229
GGAGG 589770 0.9242484 9.193333 30-34
ACTAA 258215 0.92420906 12.214895 240-247
GTCTA 416265 0.9209826 5.899908 180-189
TACTG 415215 0.9186593 5.2271466 180-189
CCAGC 448065 0.9094351 6.4133973 80-89
TGCAT 407195 0.90091527 6.1758437 210-219
ATCAA 249860 0.8943046 14.530586 70-79
CGTCC 523010 0.89037 11.8787775 70-79
ACCCA 321605 0.885708 11.297937 240-247
CCCCC 496040 0.8816443 5.2299933 80-89
AAAGA 224955 0.8806695 5.153824 8
TATGA 318235 0.8764456 8.4711895 240-247
GAGTT 428150 0.8690322 5.0106473 160-169
TACCG 398465 0.8443962 13.48851 70-79
TGAAG 348555 0.84349287 10.565832 35-39
GGTCC 535235 0.8359176 15.498403 50-59
TTTAT 316145 0.8311131 5.9225526 220-229
TGAGA 333850 0.80790716 5.1922216 240-247
ATTTA 257600 0.8074021 5.6676264 220-229
CCCAA 282220 0.7772408 7.2869215 240-247
GTACC 364935 0.773342 5.0132113 80-89
TCGAA 289605 0.76393723 15.19441 70-79
AGCCA 301950 0.76288885 6.902488 80-89
ATGGG 427185 0.7618842 18.182144 15-19
GCGTG 520730 0.74608797 5.504607 160-169
AAAGG 257855 0.7439706 9.567083 10-14
TATTA 237245 0.74360293 5.2584386 230-239
CATTG 335815 0.7429877 6.2360463 50-59
GTAGC 381620 0.7419005 5.369558 80-89
AAAGC 233065 0.7329913 11.341994 70-79
CCGAA 287850 0.7272647 6.206686 90-99
CATAT 238925 0.7172659 6.2131042 240-247
CTAAT 238345 0.71552473 9.216768 240-247
AAGGT 295260 0.71452045 11.587329 10-14
TAACC 244555 0.70318514 5.8346157 80-89
AGCAG 295805 0.68563056 5.178194 80-89
TAGGG 381995 0.6812879 5.1650066 10-14
ATCCC 288515 0.66644764 5.2324777 240-247
ATAGG 274330 0.66387045 5.522899 10-14
TAGGT 324930 0.65952265 6.2756677 10-14
CCAAT 227645 0.6545628 7.326107 240-247
CCCAT 282090 0.6516064 5.2920313 240-247
CCCGA 316665 0.6427332 5.1490664 90-99
GGTGG 483145 0.63505787 12.6008 25-29
AATCC 213560 0.6140632 6.8791 240-247
GAGGC 353805 0.60438204 7.3595934 30-34
AATGG 249210 0.6030808 6.9986606 10-14
CATCC 256535 0.59257627 7.895254 240-247
GAGGT 330595 0.589616 8.088246 10-14
AAGCA 184545 0.5803955 7.608326 70-79
GAAAA 145330 0.56894803 5.675975 8
GAAGG 265845 0.5652904 6.099196 10-14
GTCGA 290055 0.56389064 10.14826 70-79
CAATC 193155 0.55539143 6.9208274 240-247
GGGTC 382750 0.5483939 5.178934 70-79
AAGGG 256015 0.54438794 7.06137 10-14
ATAAG 160975 0.5285737 5.9488406 9
GGGCC 382185 0.52447605 9.458093 15-19
TGATC 236055 0.52226955 6.1037517 240-247
TGGGC 350215 0.50177866 10.271167 15-19
AGAGG 228705 0.4863162 5.155929 10-14
AAGAA 123640 0.48403448 5.4245687 9
CGGTT 289605 0.47222662 9.37307 10-14
TAAGG 191570 0.46359372 6.4194736 10-14
TAAAA 102750 0.4577898 5.3866906 8
AGAAG 157090 0.45324054 5.66186 9
AAATG 137920 0.45287088 5.2513623 9
GATCC 213205 0.4518075 6.0480213 240-247
TTGCC 251090 0.44628817 5.2035446 60-69
GGTCG 293220 0.4201177 5.147792 70-79
CGATG 202705 0.3940751 7.7898803 15-19
TGGGT 262105 0.39208314 5.571719 10-14
TAAAG 112465 0.36928743 6.0745487 9
AAGCT 123980 0.32704177 5.096294 30-34