FastQCFastQC Report
Thu 4 May 2017
HCGCLBCXY_s2_1_illumina12index_14_SL248361.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s2_1_illumina12index_14_SL248361.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6764433
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 7662410 8.105423 57.66563 110-119
ATACA 7688645 7.8697615 38.94008 110-119
CACAG 10502180 7.6062207 43.70016 110-119
TCCCT 12304295 7.188601 114.83569 30-34
CAGTA 9402970 7.038062 45.637543 60-69
CCCTG 12504305 6.300368 77.01508 30-34
AGTAA 6833085 6.2336984 46.77779 110-119
GGTGA 12163350 5.9338255 101.136665 45-49
GGAGA 10255210 5.9002237 90.27307 35-39
GTAAT 7528405 5.8235736 38.882435 110-119
AATAC 5441710 5.5698967 41.216167 110-119
ACAGT 7216625 5.401597 35.147934 110-119
ACTCC 7543835 5.197831 133.10748 30-34
CTCAG 8207290 5.0401907 37.463722 140-149
GTGAC 8930495 4.8880973 82.34898 45-49
GTAGT 8614695 4.873072 23.532085 70-79
ACCAG 6686420 4.8426504 97.48151 45-49
AGACG 7368755 4.756645 97.3041 40-44
CCCCA 7130525 4.753932 99.928474 60-69
CACTC 6818660 4.698173 107.15964 25-29
GAGAC 7205395 4.6511946 138.2833 40-44
GCACA 6225380 4.5087423 33.219795 110-119
GGCCC 10034505 4.3603454 61.529 60-69
CTCCC 7656620 4.3283877 75.88988 30-34
ATGCA 5661720 4.237761 101.58703 25-29
GACCA 5801970 4.2020864 109.361946 45-49
AAAAA 2843885 4.184148 22.712484 130-139
TGACC 6680135 4.1023474 138.03394 45-49
CGTGT 8825910 4.096204 21.55311 120-129
GTGTC 8638245 4.0091057 22.73647 120-129
CCCAG 6652390 3.952996 86.464325 60-69
TACAC 4657495 3.9113193 29.692743 110-119
AGTAG 5693930 3.7985458 29.704565 70-79
TGCAC 6127015 3.76267 124.410675 25-29
AGATA 4085715 3.7273228 20.586466 150-159
TAGTA 4814085 3.7239199 21.957418 80-89
TGAGG 7607800 3.7114246 67.39054 35-39
CAGGG 7842945 3.7022195 39.541424 50-59
ATGGT 6505315 3.6798599 24.965178 190-199
AGGAG 6361935 3.6602702 91.5165 35-39
CCACT 5294815 3.6482184 25.972239 240-247
GGGTT 8738455 3.6147134 46.077003 50-59
GGATG 7357530 3.5893319 75.48134 20-24
CTGAG 6537465 3.5782752 104.60081 35-39
GACGG 7542995 3.5606298 104.68073 40-44
TGTCC 6836765 3.560045 20.84284 120-129
CCTGA 5779810 3.5494475 91.75834 30-34
CAGAT 4725090 3.5366993 19.054306 150-159
ACGGT 6443030 3.5265863 92.84172 40-44
GCACT 5710500 3.5068839 96.94439 25-29
GAGGA 5990290 3.4464483 118.871124 35-39
CTTCA 4831245 3.440231 16.59172 200-209
CTCTC 5833665 3.408232 14.85166 100-109
GCCCC 6905475 3.366676 73.143745 60-69
TGGTG 8087540 3.3454583 17.884663 190-199
CCAGG 6303150 3.3382852 44.09203 50-59
CCTGG 7396985 3.3218362 30.260979 50-59
CGGAT 5988470 3.2777832 86.173874 20-24
AGGCT 5967670 3.2663984 20.895287 140-149
TTCCC 5497350 3.2117448 51.930412 50-59
AGGGT 6565900 3.2031393 55.202293 50-59
CCCTT 5471465 3.1966226 23.604183 20-24
TCAGG 5837625 3.1952183 22.040031 140-149
TCTCA 4477490 3.1883295 23.419044 130-139
TCTTG 5859740 3.1534195 17.422947 170-179
GATGC 5727485 3.1349332 105.3833 25-29
AGTCA 4185675 3.1329508 35.545418 70-79
CCAGT 5070820 3.1140487 42.69072 60-69
GGTTG 7445150 3.0797303 80.20896 15-19
TCGCA 4932180 3.0289083 23.740356 100-109
CTTGG 6499840 3.0166485 14.782464 170-179
CGCAC 5064515 3.0094454 24.640022 100-109
GTTGG 7206335 2.9809427 56.51359 15-19
CCGTG 6619800 2.9728181 22.20746 120-129
GGTTC 6383145 2.9624891 51.881817 50-59
TGGCC 6547345 2.94028 45.586685 60-69
ACAGC 4047395 2.931333 13.170747 120-129
GTTCC 5557445 2.8938766 58.49275 50-59
TCCAT 4043980 2.8796358 14.191111 70-79
ACCAC 3530025 2.8684685 16.596922 230-239
TCTCT 4729185 2.8554368 14.818862 180-189
TGTCT 5261530 2.8314927 18.489347 180-189
TCAAA 2757910 2.8228762 57.34634 70-79
GCGGA 5910160 2.789859 95.70412 20-24
GCCCT 5467185 2.754673 16.40708 200-209
CAGCT 4428605 2.7196572 13.195661 150-159
GATGG 5561125 2.7129653 14.126544 15-19
GCCGT 6025345 2.7058604 20.840084 120-129
CGGTG 6712180 2.6866078 66.63333 40-44
TTCTT 4255780 2.655608 19.672977 170-179
TCCTC 4519790 2.64062 18.282408 130-139
AGATG 3955955 2.6391046 17.956821 180-189
CGGCC 6020425 2.6160865 11.313002 120-129
ACTAC 3104090 2.6067848 14.31241 230-239
TGTTC 4823860 2.5959606 13.846296 140-149
TTGGG 6230985 2.5774834 84.87531 15-19
GGCTG 6414415 2.5674248 13.634632 140-149
TACCA 3053455 2.5642622 17.169977 230-239
GGCGG 7327635 2.5294328 46.99315 20-24
GACTC 4101500 2.518778 17.536179 190-199
TCAGC 4072430 2.500926 15.38619 140-149
GAGAT 3684870 2.4582577 17.349363 180-189
CTGGC 5404390 2.4270022 37.182938 60-69
CACGG 4565575 2.4180279 9.971521 120-129
CTGGA 4402100 2.409485 13.906463 180-189
ACACA 2426140 2.4028635 15.868775 120-129
TCACA 2834815 2.3806503 8.739944 140-149
TGGGG 6629635 2.3650887 57.772903 15-19
TCATT 3195960 2.3519518 17.782335 150-159
TAGTC 3681820 2.3367286 31.975128 70-79
ACAGA 2645365 2.3351567 10.291978 140-149
CTACT 3274965 2.3320358 9.306281 230-239
CATTT 3150215 2.3182874 17.905027 150-159
TGGAG 4728635 2.3068392 13.290778 180-189
TTCAC 3228995 2.2993016 18.148428 200-209
GCTGT 4952935 2.2987125 10.7961855 140-149
GATAC 3067060 2.2956746 16.051292 150-159
GTTCT 4251495 2.2879422 17.545614 160-169
TTCAT 3108515 2.2876 17.704084 140-149
ACTCT 3189265 2.2710106 13.050561 190-199
TGAAT 2933220 2.2689831 15.713488 190-199
TGGAA 3389425 2.2611597 10.802376 170-179
ATCTC 3139670 2.2356951 9.391414 100-109
GTGAA 3348780 2.2340446 17.612598 190-199
TACAG 2982145 2.2321162 15.603617 160-169
TTGTC 4133375 2.2243762 14.710218 170-179
AGGGA 3840695 2.209702 6.802632 150-159
ATTTG 3367980 2.209092 15.925779 150-159
CAGGC 4170685 2.2088854 17.3885 140-149
AGCTC 3596610 2.2087195 14.000793 140-149
TCTGC 4230230 2.2027686 12.393666 210-219
TACTA 2529750 2.1955686 12.180401 240-247
GAATC 2905305 2.174602 15.948251 190-199
CTCTG 4173605 2.1732826 15.011795 180-189
CAGCC 3647765 2.1675818 12.210851 120-129
AATCG 2859135 2.140044 15.432616 190-199
ACACG 2950820 2.1371367 13.481564 120-129
GTCCT 4081395 2.125267 15.410816 130-139
CACTA 2528750 2.1236198 14.303615 240-247
CTCGC 4169255 2.1007035 16.653254 100-109
GAACT 2797810 2.0941427 8.889057 160-169
GGGGC 6062595 2.0927525 53.695744 15-19
GTTCA 3269310 2.074922 15.534919 140-149
GATAT 2659315 2.0571046 7.454927 180-189
GGGCG 5952460 2.054735 66.92855 20-24
CTCCA 2961735 2.0406861 9.492542 150-159
AACCA 2058245 2.038498 15.176361 80-89
AATAA 1622585 2.0242312 8.398552 80-89
TTGGA 3573505 2.0214233 15.428788 170-179
TCTCG 3872780 2.0166368 16.928932 100-109
CCTTC 3447300 2.0140338 13.672641 200-209
CATAG 2682645 2.0079424 8.978114 210-219
GTCAA 2681860 2.007355 40.595272 70-79
TTGCA 3149230 1.9987112 14.643189 150-159
TGTAG 3496250 1.9777225 6.8451004 210-219
ATATG 2552905 1.9747915 7.7285337 180-189
CGTAG 3594745 1.9675802 10.16586 210-219
AGTAT 2538160 1.9633856 13.790776 220-229
TCGGC 4259590 1.9128957 8.328851 190-199
TTTGC 3534175 1.9019166 12.959648 150-159
GTCTC 3647270 1.8992091 11.789846 180-189
AATAT 1779570 1.8824584 10.309569 110-119
CAGAG 2878795 1.8583069 7.445832 140-149
ATATA 1736570 1.8369724 10.158296 240-247
TGCAG 3350355 1.8338135 13.891548 150-159
GCAGA 2828940 1.8261249 15.084587 150-159
CTGTT 3372605 1.8149676 13.305375 140-149
ACGGC 3403710 1.8026788 13.2588215 120-129
CCATC 2606375 1.7958369 15.126636 240-247
CAAAA 1485635 1.793355 20.939953 70-79
ATTGT 2727295 1.7888601 8.693907 170-179
GAGTC 3266230 1.7877678 12.188797 210-219
GTCTG 3785115 1.7567142 9.236424 210-219
CTGTG 3770110 1.7497503 6.0270224 210-219
AGTCT 2711680 1.7210131 13.021216 210-219
TCAGA 2297035 1.7193159 11.1650505 130-139
TGTGC 3697475 1.7160394 8.251865 220-229
CAAAG 1920020 1.694869 29.924877 70-79
TGCTC 3244090 1.6892649 6.783393 150-159
CACCA 2078625 1.6890732 10.581048 230-239
TTCAG 2642145 1.6768817 8.041163 150-159
ACCAT 1977725 1.6608742 11.588404 45-49
GTGCT 3566875 1.6554265 7.5613203 220-229
ATAGT 2125300 1.644019 7.8932858 220-229
GTCAC 2651270 1.6281753 6.6064 130-139
TGACT 2562420 1.6262827 11.274281 190-199
ATGTA 2098375 1.6231912 7.883539 150-159
AGATC 2149385 1.6088008 8.283407 130-139
GCTCT 3086185 1.6070405 10.681096 130-139
TCTGG 3440695 1.596865 11.304042 180-189
TTCCA 2230745 1.5884681 10.393734 230-239
GCTCC 3128925 1.5765271 7.5003705 150-159
ATCGG 2843810 1.5565566 9.389966 190-199
CCTCA 2249805 1.5501541 10.174982 130-139
CCACC 2315390 1.5436741 8.297896 230-239
ACTTC 2163105 1.540303 7.8964763 220-229
TAGTT 2321445 1.5226591 9.744058 220-229
CTGCT 2905570 1.5129906 6.308672 90-99
GTTGT 3150655 1.511198 5.401075 210-219
TGGTT 3138555 1.5053943 18.459835 10-14
TGATA 1940280 1.5008974 6.845712 180-189
AACTG 2005135 1.5008307 8.089566 160-169
GAACC 2066480 1.4966516 12.555106 70-79
CCAGA 2063820 1.4947251 20.532757 60-69
GCTCA 2431515 1.4932212 10.76843 140-149
CCATA 1777735 1.4929247 15.057791 70-79
GGAAT 2236585 1.492075 10.326089 170-179
AATTG 1928380 1.4916922 10.735682 170-179
GCGTA 2691390 1.4731297 10.47263 210-219
CTGGT 3168600 1.4705827 5.752368 160-169
TCCCA 2132775 1.4695184 10.440228 240-247
GAATT 1872725 1.4486403 11.860187 170-179
AGAAC 1639855 1.4475574 8.792899 160-169
ACTGA 1917500 1.4352363 7.085082 180-189
AGCCG 2690195 1.4247857 10.893567 120-129
ATCAT 1616690 1.4031243 7.1169324 230-239
GACGT 2554990 1.3984712 7.2859197 170-179
GTGTT 2915535 1.3984238 8.161431 160-169
GTAGG 2850460 1.3905817 5.680645 160-169
CCTTG 2665650 1.3880593 14.874295 20-24
GGACG 2937710 1.3867301 5.414956 170-179
GAGAA 1753505 1.3796046 8.0833025 160-169
GGTCA 2514030 1.3760519 5.651403 130-139
ACGGA 2123645 1.3708459 10.155603 200-209
ATAAC 1336035 1.3675071 8.960492 80-89
CCCAC 2040745 1.3605678 9.658904 240-247
TTGTA 2054970 1.3478754 6.6330204 210-219
TCCAC 1949935 1.3435385 9.753297 240-247
GGCCG 3458570 1.3394876 10.81521 120-129
GATCT 2108835 1.3384074 6.6699047 130-139
CCTCG 2647290 1.3338524 8.569647 130-139
CACTG 2169330 1.3322103 5.482408 230-239
GTCCC 2625770 1.3230095 15.778431 50-59
TTGGT 2717430 1.3034035 17.05039 25-29
GAGGG 3096520 1.302789 6.2782373 200-209
ATATC 1476055 1.2810674 8.110704 70-79
GTGGA 2624810 1.2804995 13.607568 25-29
AATAG 1399425 1.2766697 7.1544776 240-247
ATAGA 1389810 1.2678981 8.185257 240-247
CTCTT 2089050 1.2613485 6.970849 200-209
CTGAT 1971530 1.2512645 6.6618714 180-189
AAAGT 1359935 1.2406437 22.053024 70-79
CTTGA 1951975 1.2388536 6.82731 200-209
ATACC 1475055 1.2387369 10.373206 70-79
GAAGA 1572880 1.2374941 16.997942 35-39
TCACG 2011315 1.2351716 10.151648 200-209
TCTGT 2285250 1.2298075 7.200458 210-219
AAATA 985330 1.2292334 6.185828 9
TATGG 2159165 1.2213742 5.7291007 180-189
AGTTC 1917745 1.2171289 5.268705 160-169
AAAAG 1130630 1.2164431 14.854073 70-79
AGAGC 1878755 1.2127655 6.3186727 140-149
TGGAC 2203535 1.2061026 8.46163 170-179
CAGCG 2276130 1.205488 7.5671535 160-169
AAGTA 1304365 1.1899482 17.082926 70-79
CTTCT 1970735 1.189911 6.583578 210-219
TAACT 1365760 1.1853423 6.71209 240-247
GGAGT 2422650 1.1818769 7.430716 210-219
GCATA 1578160 1.1812426 7.8129683 210-219
GGCTC 2624810 1.1787491 7.5747843 130-139
ACCGT 1918515 1.178182 12.854526 45-49
AAGAG 1476515 1.1616771 13.155673 35-39
ACTAT 1336800 1.160208 6.2789145 230-239
CAGAC 1600565 1.159212 10.712569 60-69
GCTTC 2213165 1.1524409 5.1279783 150-159
AGCTT 1813180 1.150765 5.6551185 150-159
GGGAG 2709215 1.1398394 5.228415 150-159
CTACC 1644665 1.1332023 8.081306 230-239
TTGGC 2433180 1.129266 7.986173 60-69
CTGAA 1488585 1.1141963 14.415613 35-39
AACTT 1281840 1.1125083 6.979611 200-209
CATCA 1318055 1.1068898 8.779099 230-239
GCTGA 2012130 1.1013374 11.448274 30-34
CATGT 1733330 1.1000868 7.285893 150-159
ATCCA 1304855 1.0958047 15.306162 240-247
CTGCC 2172035 1.0943925 6.5419855 200-209
GGAGG 2592260 1.0906333 10.2887945 30-34
CTTCC 1861105 1.0873228 7.597362 230-239
CGACT 1769535 1.0866919 6.671337 190-199
CAATA 1060765 1.0857528 6.9861407 100-109
TATCA 1247400 1.0826178 7.765358 70-79
GTGGT 2610345 1.0797846 7.7162824 50-59
AAAAT 864705 1.0787495 6.264403 8
TATAA 1016790 1.0755771 9.310261 240-247
ACGTG 1962700 1.0742819 7.0566316 170-179
CCATG 1746900 1.0727913 7.440505 150-159
AGCGT 1940695 1.0622375 7.4579186 160-169
AGGTT 1877380 1.061977 23.92183 10-14
ATAAA 844760 1.0538675 6.224345 5
TTGAG 1862335 1.0534664 6.43391 200-209
CGAAC 1446880 1.0479054 12.917442 70-79
ACTCG 1702370 1.045445 6.8749785 190-199
TCGAC 1702095 1.045276 7.103725 190-199
TCATA 1204315 1.0452243 5.4917583 240-247
ATGAT 1341465 1.0376859 10.862552 240-247
TGCGT 2230025 1.0349796 8.012987 210-219
CGGCT 2301215 1.033429 6.6810393 130-139
TGCCC 2044350 1.0300577 5.5650654 200-209
CTCGG 2290710 1.0287114 7.707612 130-139
CTCGA 1668850 1.0248599 6.3640914 190-199
TTCTG 1888900 1.0165117 6.0824647 210-219
GGGGT 2846565 1.0154978 8.909613 10-14
TAGGC 1846005 1.010409 6.957096 160-169
ACTGG 1845625 1.0102011 5.6706676 160-169
AGAGA 1274845 1.0030093 12.073261 35-39
TAGAT 1290955 0.9986141 6.4015517 240-247
TGCTA 1570785 0.99692494 5.104793 220-229
TCTAC 1397175 0.99489987 6.4480767 180-189
TCCCC 1757250 0.9933965 5.8018355 90-99
CGGAG 2091485 0.9872741 6.981573 210-219
CCAGC 1657160 0.9847207 6.9020214 80-89
ACAAT 952730 0.97517294 8.300269 100-109
TATGT 1485960 0.97465605 6.0806494 220-229
GTCCA 1585815 0.9738672 11.793958 70-79
CTGCG 2159370 0.96972936 6.6185727 210-219
AGCTG 1770095 0.9688598 6.063471 80-89
AGTTT 1472350 0.96572924 5.2934 220-229
TATTT 1264740 0.9618933 6.9557195 220-229
TACTG 1515165 0.9616248 5.7419868 180-189
GTCTA 1514275 0.9610599 6.0982304 180-189
TATGA 1236635 0.956595 8.40241 240-247
ACCCA 1176730 0.95620096 11.139249 240-247
AAAGA 886055 0.9533052 6.0974607 8
GAGCT 1734590 0.9494262 5.45686 140-149
TGGTC 2031595 0.9428859 8.321533 50-59
TGTAA 1202200 0.9299579 6.551495 230-239
TACAA 902590 0.9238517 6.5064254 100-109
ATGGG 1855380 0.90513724 21.218554 15-19
TGCAT 1414975 0.8980375 6.149648 210-219
CCATT 1257880 0.8957107 6.152483 50-59
ACTAA 872335 0.892884 11.554812 240-247
GGAAG 1545800 0.88935924 5.6708965 30-34
GGTGG 2483935 0.88613135 14.421969 25-29
GTATT 1340160 0.87902445 6.52207 220-229
CCCAA 1070405 0.8698022 8.151245 240-247
ATCAA 848010 0.867986 13.235832 70-79
TTTAT 1135665 0.8637258 6.1720386 220-229
TGAGA 1273750 0.84974664 5.2294683 240-247
CATAC 1010645 0.8487299 13.266366 70-79
ATTTA 945840 0.8483707 5.988628 220-229
CTATA 967535 0.8397231 5.0636835 90-99
TCCAG 1344170 0.8254702 5.164422 240-247
TGAAG 1215175 0.81066996 9.053308 35-39
ACGTC 1311380 0.8053335 7.951373 70-79
CGTGG 2011325 0.80505013 5.324373 50-59
TCGAA 1061495 0.79452217 15.682745 70-79
CCTCT 1343415 0.7848702 5.062172 100-109
AGCCA 1068230 0.7736674 7.3712244 80-89
AGCAG 1181250 0.76251525 5.6736603 80-89
CATAT 855855 0.7427961 6.720397 240-247
AAGGT 1102280 0.7353552 10.09008 10-14
AAAGG 931395 0.7327933 8.549302 10-14
CCAAT 867885 0.7288415 7.9158716 240-247
TAACC 846930 0.7112436 6.3120375 80-89
GTAGC 1293330 0.7079029 5.2166224 80-89
CATTG 1113145 0.7064761 5.537862 50-59
GAGGT 1446550 0.7056917 7.2973347 10-14
CCGTA 1148550 0.7053377 6.573106 80-89
GAAAA 653995 0.7036322 6.9239197 7
CTAAT 809790 0.7028163 9.077855 240-247
CCCAT 1014410 0.6989459 5.6951056 240-247
GACCG 1309515 0.6935476 12.0604315 45-49
ATCCC 998105 0.6877114 5.3777 240-247
GCGTG 1714175 0.6861133 5.186856 160-169
CCGAA 937530 0.6790077 5.170465 90-99
ATAGG 1012625 0.6755444 5.1077375 10-14
AATCC 797355 0.669611 7.4378695 240-247
AAAGC 746510 0.65897053 9.871721 70-79
GAGGC 1388380 0.65537727 7.68628 30-34
CATCC 940540 0.64804816 9.12072 240-247
AATGG 957285 0.63862586 6.636862 10-14
TAGGT 1119695 0.6333776 5.2378907 10-14
CAATC 739655 0.62115514 7.6016116 240-247
TGGGC 1542320 0.6173268 11.699255 15-19
GGGCC 1586400 0.61440516 10.865098 15-19
GGGTC 1527580 0.61142707 5.716856 70-79
GTCGA 1109415 0.6072372 10.991051 70-79
CGTCC 1169795 0.58940804 6.7778745 70-79
AAGGG 1000825 0.5758137 6.3491697 10-14
GGTCC 1268010 0.5694376 8.5163355 50-59
AAGCA 609235 0.5377931 6.356938 70-79
TGGGT 1297595 0.5367578 5.3086157 10-14
TGATC 838025 0.53186655 6.300511 240-247
ATAAG 567895 0.5180802 5.9475794 9
AAGAA 479775 0.51618916 5.0863104 8
TACCG 828225 0.50862247 6.7637715 70-79
TAAAT 478900 0.5065883 5.004747 8
TAAAA 400670 0.49984977 7.435319 8
TAAGG 723175 0.48244593 5.9686937 10-14
GATCC 783705 0.4812822 6.4379416 240-247
CGGTT 1012895 0.4700959 8.291443 10-14
CGATG 842235 0.4609965 10.1024275 15-19
GGTCG 1095975 0.43867344 5.72086 70-79
GGAAA 474030 0.37295243 5.0438046 6
ATTAA 348580 0.36873364 5.0282617 2
AAGCT 476310 0.35651496 5.225486 30-34
TAAAG 387280 0.35330844 5.04964 9
AATTA 284430 0.30087474 5.621359 6