FastQCFastQC Report
Thu 4 May 2017
HCGCLBCXY_s1_1_illumina12index_9_SL248373.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s1_1_illumina12index_9_SL248373.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11999592
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 14303815 8.68978 58.85171 110-119
ATACA 14203340 8.299093 38.80516 110-119
CACAG 18687110 7.6655045 44.077145 110-119
TCCCT 23098970 7.264122 119.26653 30-34
CAGTA 17007255 7.253532 44.47817 60-69
AGTAA 12900385 6.8523593 48.375008 110-119
GGAGA 18658150 6.3250165 99.733475 35-39
GTAAT 14188960 6.291911 39.790573 110-119
CCCTG 22647250 6.2271094 80.83391 30-34
GGTGA 21793155 6.16748 108.703316 45-49
AATAC 10102780 5.903113 42.488705 110-119
GTAGT 16921840 5.4771514 30.01853 80-89
ACAGT 12799780 5.459059 35.987316 110-119
AGACG 14384360 5.3639703 105.17312 40-44
CTCAG 15034340 5.148463 37.824574 140-149
GTGAC 16460770 5.124371 87.89909 45-49
ACTCC 13549805 5.1042147 136.48103 30-34
GAGAC 12790265 4.769528 147.4163 40-44
CCCCA 13073150 4.7365203 100.90421 60-69
CACTC 12527310 4.719041 111.53199 25-29
TAGTA 10517295 4.6637588 37.67173 80-89
ACCAG 11218025 4.601665 93.760254 45-49
ATGCA 10610625 4.5253925 110.56529 25-29
GCACA 11000840 4.512575 35.168514 110-119
CTCCC 14347210 4.3395157 78.068375 30-34
AGTAG 11018400 4.2719984 29.299591 60-69
TGACC 12458040 4.2662163 144.99292 45-49
CGTGT 16228200 4.2175026 21.179392 120-129
GGCCC 17482245 4.202897 60.760357 60-69
GTGTC 15929085 4.139766 22.020197 120-129
CCCAG 12246240 4.0334735 89.364075 60-69
TACAC 8530595 4.0021873 28.54629 110-119
AGATA 7517205 3.9929497 20.58532 150-159
GACCA 9708470 3.9824412 106.66079 45-49
TGCAC 11435525 3.9160595 132.83087 25-29
ATGGT 12036265 3.89582 26.19811 190-199
AGGAG 11353785 3.8488743 100.86123 35-39
TGAGG 13522630 3.8269157 73.12095 35-39
CAGAT 8898775 3.7952948 19.650003 150-159
GACGG 13936310 3.793314 113.521255 40-44
CCTGA 10988100 3.7628405 98.58496 30-34
CTGAG 11978890 3.7291255 110.2846 35-39
ACGGT 11973025 3.7272995 99.63628 40-44
GAGGA 10948725 3.7115612 131.24681 35-39
GGATG 13096485 3.7063162 83.33897 20-24
GCACT 10734860 3.6761189 102.81574 25-29
TGTCC 12671410 3.622536 19.883331 120-129
CAGGG 13188170 3.5896785 38.302963 50-59
CCACT 9375685 3.5318232 25.919542 240-247
GGGTT 14908580 3.5222366 44.08153 50-59
CGGAT 11221090 3.493216 92.97744 20-24
AGGCT 10995040 3.422845 21.37865 140-149
GCCCC 12735230 3.3679152 73.645386 60-69
CTTCA 8541090 3.3452291 16.000996 200-209
CTCTC 10629630 3.3427866 13.574155 100-109
GATGC 10722280 3.3379326 112.43186 25-29
TCAGG 10716760 3.336214 22.618843 140-149
CCGTG 13059950 3.2644486 21.696016 120-129
CCCTT 10366950 3.2601795 19.042902 50-59
TCTCA 8250620 3.231463 21.820047 130-139
GGTTG 13670015 3.2296185 85.939735 15-19
TGGTG 13621795 3.2182264 18.355013 190-199
CCAGG 10630200 3.1828423 41.789314 50-59
CCTGG 12649250 3.1617906 28.587711 50-59
TCTTG 10628165 3.1590955 16.985067 170-179
TCGCA 9201580 3.1510522 22.658398 110-119
GTTGG 13195840 3.1175923 61.78562 15-19
CGCAC 9434540 3.1074007 23.042364 100-109
CTTGG 11878500 3.0870707 14.344693 170-179
AGGGT 10837615 3.0670536 53.762093 50-59
ACAGC 7383225 3.028619 13.259177 120-129
GCGGA 11001830 2.9945798 105.42803 20-24
AGTCA 6982755 2.9781194 33.52147 70-79
TGGCC 11781090 2.9447858 45.167786 60-69
TCCAT 7499645 2.937334 20.382387 70-79
TGTCT 9692910 2.8811023 18.790594 180-189
TTCCC 9134430 2.8725786 47.306908 50-59
CCAGT 8355335 2.861258 40.03167 60-69
CGGTG 12531385 2.847506 71.46555 40-44
ACCAC 6290235 2.8383648 16.388098 230-239
TCAAA 4828075 2.821072 57.25865 70-79
TCTCT 8621695 2.819031 14.23944 180-189
AGATG 7269165 2.818364 18.141403 180-189
CAGCT 8176030 2.7998557 13.6401205 150-159
GGTTC 10757740 2.7957997 48.662643 50-59
ACTAC 5839325 2.7395594 15.235524 240-247
GCCGT 10901420 2.7249055 20.45007 120-129
AAAAA 3105770 2.707304 12.843874 130-139
TTGGG 11386520 2.69013 92.81676 15-19
GATGG 9495890 2.6873448 14.395086 190-199
GGCTG 11818090 2.685424 13.758353 140-149
GGCGG 13516455 2.6853993 51.71785 20-24
CGGCC 11129000 2.6755168 11.32863 120-129
TACCA 5634805 2.6436071 17.626589 230-239
TCCTC 8363965 2.6302843 17.557768 130-139
GCCCT 9521325 2.6179929 16.176357 200-209
TTCTT 7674855 2.6091201 19.252707 170-179
TGTTC 8769095 2.6065092 13.661787 150-159
GAGAT 6708890 2.6011372 17.566206 180-189
GTTCC 8787115 2.5120835 53.79466 50-59
CACGG 8382290 2.5097842 10.56311 120-129
GACTC 7268210 2.4889755 16.6605 190-199
TGGGG 11994190 2.4776144 63.372818 15-19
CTGGA 7947710 2.4741864 13.785508 180-189
TCAGC 7213235 2.4701495 15.111768 140-149
ACAGA 4762965 2.4333136 10.412002 140-149
GCTGT 9353825 2.4309402 10.694174 140-149
ACACA 4314085 2.424444 16.445776 120-129
TCATT 5919195 2.4104161 17.933702 150-159
GATAC 5577205 2.3786573 15.710586 150-159
CATTT 5834660 2.3759916 17.963324 150-159
GTGAA 6110815 2.3692546 17.986486 190-199
TACAG 5516075 2.3525858 15.950452 160-169
CTACT 5969670 2.3380992 9.376095 230-239
TACTA 4773545 2.3284976 13.007994 240-247
ATTTG 6280935 2.3251495 16.057116 150-159
TGAAT 5241350 2.324209 15.735894 190-199
TTGTC 7809205 2.3211927 13.89868 170-179
CAGGC 7744890 2.3189373 17.951395 140-149
TTCAT 5685600 2.3152912 16.777035 140-149
TTCAC 5870445 2.2992365 17.86167 200-209
GTTCT 7712420 2.2924254 16.530663 160-169
CTGGC 9135740 2.2835577 34.74587 60-69
TGGAA 5870165 2.275951 10.722224 170-179
TGGAG 8034375 2.2737348 13.442397 180-189
AGCTC 6607900 2.2628546 13.643784 140-149
CGTAG 7241905 2.2544637 12.162887 80-89
AATCG 5279230 2.2515721 15.449086 190-199
GGGGC 11253045 2.2357135 58.72362 15-19
GAATC 5234995 2.232706 15.993468 190-199
ACTCT 5691375 2.2291014 12.691921 200-209
TAGTC 6256615 2.227662 29.849396 70-79
AGGGA 6546545 2.219245 6.857087 160-169
CTCTG 7631185 2.1816232 14.192553 180-189
GGGCG 10976075 2.180686 72.265205 20-24
CAGCC 6599805 2.1737397 11.767872 120-129
ATCTC 5492940 2.1513817 7.973208 100-109
GTCCT 7512865 2.1477976 15.375197 130-139
TCACA 4573130 2.1455152 8.672299 140-149
TCTGC 7491410 2.1416638 12.138261 210-219
CATAG 5013450 2.138218 8.838702 220-229
AACCA 3790575 2.1302402 15.327316 80-89
CTCGC 7730050 2.1254618 15.674916 100-109
CACTA 4511780 2.1167324 14.7079525 240-247
AGTAT 4760115 2.1108117 13.814521 220-229
TTGGA 6486530 2.0995178 15.284129 170-179
GTTCA 5885085 2.0953794 14.920841 140-149
AATAA 2874695 2.0919635 9.399651 80-89
TTTGC 6944270 2.0641012 12.564864 150-159
ACACG 5002195 2.0519142 14.677279 120-129
TTGCA 5741855 2.044382 14.590676 150-159
ATATG 4544630 2.0152576 7.4868093 180-189
GATAT 4516990 2.003001 7.2009783 180-189
TGTAG 6161035 1.994164 6.7786984 160-169
TCGGC 7928520 1.9818026 7.786648 190-199
GTCAA 4628175 1.9738996 39.814426 70-79
CCTTC 6272560 1.9725832 13.054583 200-209
GAACT 4615110 1.9683273 8.314381 160-169
AATAT 3232150 1.963579 10.59893 110-119
TCTCG 6799585 1.9438831 15.465524 100-109
CCATA 4106625 1.9266511 24.607046 70-79
CAGAG 5138980 1.9163411 7.189549 140-149
GCAGA 5103095 1.9029595 15.1863785 150-159
GAGTC 6073990 1.8908823 12.139939 210-219
ATTGT 5107770 1.8908536 8.324209 180-189
GTCTC 6605020 1.8882605 11.457036 180-189
ACGGC 6305395 1.8879308 13.555708 120-129
TCAGA 4417840 1.8841925 11.097579 130-139
CTCCA 4974625 1.8739425 9.42883 150-159
TGCAG 5987530 1.8639666 13.893107 150-159
CAAAA 2645975 1.8519589 22.72356 70-79
CTGTT 6216995 1.8479279 12.871292 140-149
ATATA 3024295 1.8373041 9.542792 240-247
CTGTG 6986075 1.815592 6.067047 210-219
ATAGT 4034310 1.7889627 8.743705 220-229
ACCAT 3810725 1.7878277 12.605504 45-49
CCAGA 4293225 1.7610928 28.29671 60-69
AGTCT 4941745 1.759504 12.935327 210-219
ATGTA 3958410 1.7553059 8.7122135 160-169
TGCTC 6130195 1.7525163 7.0379486 150-159
TGTGC 6740520 1.7517755 8.5268345 220-229
ACCGT 5085975 1.7416761 21.900915 45-49
GTGCT 6528845 1.6967638 7.335887 220-229
GACGT 5435805 1.6922101 9.736245 60-69
TCTGG 6392845 1.6614189 11.258774 180-189
GTCTG 6378360 1.6576546 9.139842 210-219
ATACC 3511585 1.6474841 18.028214 70-79
CAAAG 3219790 1.6449331 28.996803 70-79
GCTCT 5739795 1.6409076 10.5522785 130-139
CACCA 3593070 1.6213136 11.265888 230-239
GCTCC 5878280 1.6162975 7.9421635 150-159
AGATC 3775435 1.6102091 8.741065 130-139
TGACT 4487835 1.5978897 10.825423 190-199
GTCCC 5787375 1.5913022 21.982887 50-59
TTCAG 4468170 1.5908878 8.315269 140-149
TAGTT 4276330 1.5830615 10.247932 220-229
GAACC 3801200 1.5592626 12.024996 70-79
AATTG 3478670 1.5425713 10.673652 170-179
TGGTT 5669040 1.5318323 19.89849 10-14
GTCAC 4460545 1.5274996 6.4842777 140-149
GCTCA 4445060 1.5221969 10.546783 150-159
GTTGT 5622185 1.5191718 5.2451544 210-219
GCGTA 4873570 1.5171821 10.555975 220-229
CCTCA 3993745 1.5044447 9.436906 130-139
ACTTC 3840005 1.5039881 7.9409924 210-219
TGATA 3360220 1.4900463 6.7578006 180-189
GAATT 3351095 1.4859998 11.499184 170-179
ATCGG 4771325 1.485352 8.866363 190-199
CCATC 3935785 1.4826112 13.876413 240-247
GGAAT 3823250 1.4823313 10.024226 170-179
CCACC 4075060 1.4764313 8.378233 230-239
AGAAC 2888245 1.4755528 8.779915 160-169
GATCT 4141570 1.4746023 6.859072 130-139
CTGGT 5664540 1.4721419 5.413872 160-169
AGCCG 4910515 1.4702823 10.770922 120-129
AACTG 3437710 1.4661707 7.825848 160-169
CAGAC 3570535 1.4646434 17.932 60-69
CTGCT 5096830 1.4570951 6.2948184 140-149
GTAGG 5119035 1.4486912 6.9390535 160-169
GGACG 5237110 1.4254851 5.260315 170-179
GAGAA 3052615 1.4177185 8.330627 160-169
ACGGA 3791905 1.4140128 9.829561 200-209
GGCCG 6403765 1.3995364 10.815272 120-129
ACTGA 3279285 1.398603 6.8403335 180-189
CCTCG 5071315 1.3944136 8.558022 130-139
ATAAC 2382250 1.3919624 9.382722 80-89
TCCCA 3690910 1.3903667 10.010838 240-247
CCTTG 4828565 1.3804028 11.14264 20-24
GGTCA 4423345 1.3770231 5.343854 130-139
TTGTA 3709330 1.3731629 6.8160434 220-229
GTGTT 5070445 1.3700858 7.8408966 160-169
AATAG 2538500 1.3483871 8.199487 240-247
TCACG 3909470 1.3387856 9.920623 200-209
ATCAT 2733400 1.333331 6.577646 240-247
CTGAT 3692045 1.3145493 6.7162523 180-189
CACTG 3833390 1.3127323 5.2588573 230-239
TTGGC 4999505 1.2993077 10.010011 60-69
TCCAC 3436655 1.2945889 8.988934 240-247
CCCAC 3569860 1.2933927 9.804748 240-247
TTCCA 3258685 1.2763065 9.253712 230-239
GCATA 2942390 1.2549186 8.482095 220-229
GTCCA 3658850 1.2529618 18.890177 70-79
ATAGA 2351335 1.2489698 8.525942 240-247
CAGCG 4163840 1.2467165 7.7067885 160-169
TCTGT 4164925 1.2379744 7.278057 210-219
TATGG 3810240 1.2332737 5.5215354 180-189
TATAC 2526305 1.2323116 5.195277 110-119
GGCTC 4874705 1.2184751 7.7051053 130-139
GTGGT 5156580 1.2182713 12.833115 50-59
GGAGT 4299570 1.2167817 7.3946276 210-219
CATAC 2589600 1.2149286 22.51428 70-79
AAATA 1668630 1.2142899 5.589948 9
TAACT 2475375 1.2074684 5.9755015 240-247
TGGAC 3873880 1.2059702 8.302275 170-179
ATATC 2471070 1.2053684 8.334357 70-79
AAAAG 1882035 1.1974863 16.315664 70-79
GAGGG 4824570 1.1937869 5.8501635 200-209
CTCTT 3643795 1.1914098 6.5717683 200-209
AGCTT 3322520 1.1829803 5.365143 150-159
TGGTC 4540535 1.1800272 14.176674 50-59
AAAGT 2214705 1.1763954 20.609146 70-79
TGCCC 4220720 1.1605332 6.548593 60-69
TTGGT 4272355 1.1544338 12.566224 25-29
CTTGA 3214225 1.1444218 6.3733807 200-209
CTACC 3026770 1.140185 8.166456 230-239
AGGTT 3519145 1.139054 26.913618 10-14
CATGT 3168950 1.1283019 7.412725 160-169
GGGAG 4558015 1.1278307 5.076903 150-159
AAGTA 2119035 1.1255779 15.550206 70-79
ACTAT 2301135 1.1224755 5.877712 230-239
AACTT 2299775 1.1218121 7.245797 210-219
AGCGT 3598930 1.120376 7.6023397 160-169
GCTGA 3584995 1.116038 9.923091 30-34
TATCA 2286155 1.1151683 7.931106 70-79
CTGCC 4033450 1.1090412 6.9109125 200-209
TAGGC 3560585 1.108439 8.051085 160-169
CCATG 3222255 1.103451 7.477294 150-159
CTTCT 3364130 1.0999677 6.779144 210-219
CGGCT 4371760 1.0927596 6.7355003 130-139
AGAGC 2900935 1.0817672 5.9365873 140-149
TATAA 1780105 1.0814402 9.310197 240-247
CTCGG 4324135 1.0808554 7.8615513 130-139
CGAAC 2616455 1.0732771 12.336802 70-79
GAAGA 2310945 1.0732666 18.35528 35-39
ACGTG 3446150 1.0728143 6.7262883 170-179
CAATA 1834335 1.0718125 6.4323974 100-109
TGCGT 4112090 1.0686798 8.048942 210-219
CGACT 3115420 1.0668658 6.448621 200-209
AAAAT 1463080 1.0647076 6.3913236 90-99
TTGAG 3262835 1.0560932 6.171332 200-209
ATAAA 1446720 1.0528023 6.4679837 7
ATCCA 2237750 1.0498557 14.683544 240-247
GTGGA 3707950 1.0493528 10.869968 25-29
TCGAC 3030965 1.0379444 6.7139535 190-199
CATCA 2193350 1.0290251 8.161116 230-239
CGTGG 4502450 1.0230914 9.034687 50-59
CGGAG 3756315 1.0224286 7.2116013 210-219
ACTCG 2971540 1.0175945 6.404579 190-199
TATGT 2746555 1.0167516 6.623743 220-229
AGTTT 2734785 1.0123944 5.859155 220-229
AGTTC 2839770 1.0110976 5.037279 160-169
TCCCC 3317590 1.0034518 5.6836762 90-99
AGCTG 3218640 1.0019886 6.116371 90-99
TGTAA 2245975 0.99594873 7.1147866 230-239
ATGAT 2243095 0.9946715 10.499689 240-247
TTCTG 3342610 0.9935511 6.2084575 210-219
AAGAG 2135600 0.99183154 14.129003 35-39
TGCTA 2780725 0.9900747 5.0249567 220-229
TCTAC 2524795 0.98886895 5.989151 180-189
GAGCT 3172760 0.9877059 5.2368884 140-149
TCATA 2019450 0.9850718 5.259948 240-247
ACTGG 3152820 0.98149836 5.4270463 160-169
CTCGA 2855570 0.977881 6.0175557 190-199
TATTT 2305100 0.97596854 6.610817 220-229
ACGTC 2838470 0.9720251 13.224066 70-79
CTGCG 3886670 0.97150713 6.500851 210-219
GTCTA 2728525 0.9714889 5.9003954 180-189
CCGTA 2836400 0.97131634 9.142179 80-89
CTGAA 2271265 0.96868616 14.698012 35-39
CTTCC 3079895 0.9685597 6.7731147 230-239
AGAGA 2074700 0.9635479 13.387514 35-39
AGGGG 3890100 0.96256256 5.00367 10-14
ACAAT 1646885 0.96228445 7.7346663 100-109
GGGGT 4642155 0.9589201 8.964316 10-14
TACTG 2687420 0.9568535 5.2450013 180-189
TAGAT 2126600 0.94301337 6.4180136 240-247
CCATT 2399035 0.9396134 6.253588 50-59
TACAA 1596655 0.93293476 6.138635 100-109
CCAGC 2827685 0.93133825 6.5622797 80-89
TGCAT 2596315 0.92441565 6.5475645 210-219
ACCCA 2035120 0.91831434 11.391219 240-247
GTATT 2452520 0.90790236 6.0682364 220-229
ACTAA 1543540 0.90189934 11.964944 240-247
GGAGG 3637460 0.90004957 8.399078 30-34
TATGA 2004310 0.8887854 8.220994 240-247
ATCAA 1492260 0.87193614 14.007298 70-79
AAAGA 1368585 0.87079245 6.9328885 8
GACCG 2875190 0.8608753 20.061146 45-49
CGTCC 3019565 0.8302625 10.95954 70-79
TTTAT 1952720 0.8267725 5.8095517 220-229
TGAAG 2123700 0.82339025 9.684606 35-39
ATTTA 1610265 0.81667423 5.604974 220-229
TGAGA 2056765 0.7974386 5.091801 240-247
GGTCC 3140235 0.78492904 14.083728 50-59
TACCG 2282635 0.7816811 12.068717 70-79
CCCAA 1728660 0.7800293 8.028301 240-247
AGCCA 1878660 0.77063155 7.153053 80-89
AAAGG 1636065 0.7598337 10.16197 10-14
TCGAA 1765075 0.752798 14.976062 70-79
TAACC 1588050 0.74504447 6.5820765 80-89
CATTG 2085305 0.742471 5.746552 50-59
ATGGG 2622055 0.74204373 16.792536 15-19
AAGGT 1896480 0.7352937 11.806521 10-14
GTAGC 2310095 0.71915126 5.2751837 80-89
GCGTG 3143450 0.714286 5.2962813 160-169
CTAAT 1458045 0.7112229 9.053319 240-247
CATAT 1451945 0.70824736 6.206088 240-247
AAAGC 1367185 0.6984704 11.132054 70-79
ATCCC 1848725 0.6964151 5.3970356 240-247
CCGAA 1690180 0.6933166 5.8055835 90-99
AGCAG 1844140 0.6876854 5.0656567 80-89
CCAAT 1456930 0.6835287 7.781089 240-247
ATAGG 1747730 0.67762107 5.6298094 10-14
TAGGG 2393360 0.67732286 5.130328 10-14
CCCAT 1769695 0.66664463 5.4933405 240-247
AATCC 1361025 0.6385342 7.252069 240-247
TAGGT 1954415 0.6325923 6.016353 10-14
GGTGG 2980810 0.6157396 11.277398 25-29
AATGG 1583425 0.6139176 7.2211986 10-14
CATCC 1625300 0.6122509 8.1836 240-247
GAAGG 1770510 0.60019374 6.0103498 10-14
GAGGT 2109615 0.5970227 8.156408 10-14
GAGGC 2125105 0.5784307 6.613307 30-34
GTCGA 1852615 0.57673407 10.43357 70-79
CAATC 1218560 0.57169574 7.3891764 240-247
AAGGG 1658835 0.5623365 7.3557982 10-14
GGGTC 2459765 0.55893224 5.3309345 70-79
ATAAG 1030840 0.5475562 6.848461 9
AAGCA 1066150 0.54467696 7.273838 70-79
TGATC 1528765 0.5443154 6.396957 240-247
GAAAA 848240 0.5397114 5.8650208 8
AGAGG 1487605 0.5042904 5.1743116 10-14
CGGTT 1911545 0.49678624 9.667781 10-14
GGGCC 2249325 0.49158773 8.470453 15-19
TAAGG 1226715 0.47561577 6.8087473 10-14
GATCC 1386070 0.4746553 6.353415 240-247
TGGGC 2084080 0.47356537 9.166382 15-19
AAGAA 729915 0.46442455 6.0166755 9
TAAAA 637530 0.46394125 6.790619 8
AAATG 857700 0.4555886 5.475489 9
AGAAG 947275 0.43994063 5.6569467 9
GGTCG 1872680 0.42552897 5.315388 70-79
TGGGT 1698000 0.40116215 5.6225986 10-14
CGATG 1190195 0.37051734 7.506791 15-19
TAAAG 686485 0.3646435 5.6854033 9
ATGAG 887335 0.34403306 5.19562 9