FastQCFastQC Report
Thu 4 May 2017
HCGCLBCXY_s1_1_illumina12index_14_SL248361.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s1_1_illumina12index_14_SL248361.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6305324
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 7127320 8.063483 57.329655 110-119
ATACA 7154895 7.837505 38.766827 110-119
CACAG 9782080 7.5943546 43.670166 110-119
TCCCT 11442570 7.1992583 115.030754 30-34
CAGTA 8758455 7.0227804 45.550915 60-69
CCCTG 11630050 6.3063054 77.13612 30-34
AGTAA 6364110 6.205308 46.551353 110-119
GGTGA 11358235 5.9340396 100.861694 45-49
GGAGA 9547795 5.8730655 89.72396 35-39
GTAAT 7003370 5.799767 38.708668 110-119
AATAC 5060805 5.543629 40.939674 110-119
ACAGT 6728975 5.3954844 35.11042 110-119
ACTCC 7031885 5.209046 133.20741 30-34
CTCAG 7642985 5.039651 37.45185 140-149
GTGAC 8318515 4.882411 82.212456 45-49
GTAGT 8024925 4.8646483 23.462261 70-79
ACCAG 6212500 4.8230977 97.07357 45-49
CCCCA 6627035 4.7531753 99.96969 60-69
AGACG 6861070 4.741356 96.88871 40-44
CACTC 6350155 4.704037 107.31967 25-29
GAGAC 6720110 4.643945 137.6635 40-44
GCACA 5803755 4.505767 33.20883 110-119
AAAAA 2789435 4.3747888 24.199806 130-139
GGCCC 9336115 4.3630357 61.55595 60-69
CTCCC 7127335 4.3417964 76.1078 30-34
ATGCA 5280120 4.2337513 101.32101 25-29
GACCA 5396330 4.189461 108.94161 45-49
TGACC 6215910 4.0986624 137.80112 45-49
CGTGT 8209165 4.09228 21.553083 120-129
GTGTC 8039715 4.007809 22.771439 120-129
CCCAG 6189685 3.9517014 86.42592 60-69
TACAC 4334200 3.904266 29.640564 110-119
AGTAG 5300690 3.7832499 29.55838 70-79
TGCAC 5711775 3.7662446 124.34034 25-29
TAGTA 4487395 3.7161887 21.93405 80-89
AGATA 3808200 3.7131748 20.470592 150-159
TGAGG 7084060 3.7010233 67.06017 35-39
CAGGG 7283595 3.6843722 39.3527 50-59
ATGGT 6071915 3.6807485 25.021849 190-199
AGGAG 5932365 3.6491325 90.94458 35-39
CCACT 4921255 3.6455436 25.94891 240-247
GGGTT 8119785 3.6029806 45.91078 50-59
GGATG 6855320 3.5815196 75.316154 20-24
CTGAG 6083225 3.5704453 104.27926 35-39
TGTCC 6361025 3.5623977 20.85096 120-129
GACGG 7018935 3.5504951 104.3394 40-44
CCTGA 5384355 3.5503502 91.74609 30-34
CAGAT 4404785 3.531883 19.036589 150-159
ACGGT 5998825 3.5209084 92.62177 40-44
GCACT 5319340 3.50748 96.89728 25-29
CTTCA 4501270 3.4438434 16.606924 200-209
GAGGA 5576800 3.4304163 118.06169 35-39
CTCTC 5422080 3.4113803 14.932402 100-109
GCCCC 6421060 3.3711512 73.278 60-69
TGGTG 7562495 3.3556952 17.974684 190-199
CCAGG 5847755 3.323195 43.886486 50-59
CCTGG 6875375 3.318493 30.21982 50-59
CGGAT 5582695 3.2766676 86.00018 20-24
AGGCT 5551010 3.258071 20.907053 140-149
TTCCC 5097960 3.2074556 51.8841 50-59
CCCTT 5089530 3.2021518 23.564598 20-24
AGGGT 6107190 3.1906636 54.88763 50-59
TCTCA 4169320 3.1898742 23.416275 130-139
TCAGG 5428895 3.1863976 22.032757 140-149
TCTTG 5445045 3.1494784 17.385347 170-179
GATGC 5340215 3.1343484 105.29897 25-29
AGTCA 3900375 3.127432 35.388767 70-79
CCAGT 4729200 3.118352 42.78003 60-69
GGTTG 6938975 3.0790215 80.15597 15-19
TCGCA 4591555 3.0275915 23.73191 100-109
CTTGG 6041270 3.0115812 14.795697 170-179
CGCAC 4712265 3.0084672 24.584692 100-109
GTTGG 6713655 2.9790406 56.416187 15-19
CCGTG 6166635 2.97641 22.209806 120-129
GGTTC 5929030 2.9556293 51.740982 50-59
TGGCC 6093100 2.9409168 45.600883 60-69
ACAGC 3767575 2.924971 13.230734 120-129
GTTCC 5173965 2.8976023 58.427097 50-59
TCCAT 3770225 2.8845334 14.28104 70-79
ACCAC 3285290 2.8653805 16.597954 230-239
TCTCT 4392130 2.8540483 14.896697 180-189
TGTCT 4890510 2.8287287 18.505987 180-189
TCAAA 2569000 2.8140945 57.11494 70-79
GCGGA 5503275 2.7838054 95.439644 20-24
GCCCT 5088920 2.7594275 16.455805 200-209
CAGCT 4117055 2.7147145 13.210808 150-159
GATGG 5191045 2.7120295 14.059428 15-19
GCCGT 5613525 2.7094436 20.876013 120-129
CGGTG 6251395 2.6857924 66.630264 40-44
TTCTT 3949935 2.650928 19.621298 170-179
TCCTC 4204250 2.645165 18.34126 130-139
AGATG 3686335 2.6310399 17.867704 180-189
CGGCC 5606765 2.6202028 11.34467 120-129
ACTAC 2891510 2.6046848 14.338356 230-239
TGTTC 4486625 2.5951169 13.839856 140-149
TTGGG 5804695 2.575709 84.77158 15-19
GGCTG 5968055 2.5640607 13.630496 140-149
TACCA 2836080 2.554753 17.140253 230-239
GGCGG 6820930 2.5256214 46.900635 20-24
GACTC 3815100 2.5156105 17.529007 190-199
TCAGC 3796640 2.5034382 15.331749 140-149
GAGAT 3440925 2.4558837 17.27445 180-189
CTGGC 5021455 2.423673 37.13045 60-69
CACGG 4252490 2.4166288 9.970097 120-129
CTGGA 4097070 2.4047058 13.867815 180-189
ACACA 2263800 2.4009922 15.863039 120-129
TCACA 2642620 2.3804836 8.762048 140-149
TGGGG 6178585 2.362847 57.687275 15-19
TCATT 2975565 2.3512545 17.740614 150-159
TAGTC 3428265 2.3347144 31.904928 70-79
CTACT 3046895 2.3311262 9.348987 230-239
ACAGA 2463880 2.3260725 10.267414 140-149
CATTT 2932530 2.317249 17.909052 150-159
TGGAG 4413015 2.3055525 13.234609 180-189
TTCAC 3009790 2.302738 18.142956 200-209
GCTGT 4613330 2.2997513 10.809369 140-149
GATAC 2861630 2.294537 15.980262 150-159
TTCAT 2894260 2.2870083 17.67807 140-149
GTTCT 3949940 2.284692 17.49607 160-169
ACTCT 2965630 2.268952 13.044009 190-199
TGAAT 2733945 2.2640877 15.643098 190-199
TGGAA 3157185 2.2533708 10.751218 170-179
ATCTC 2929785 2.2415276 9.444351 100-109
GTGAA 3125175 2.2305243 17.582766 190-199
TACAG 2777540 2.2271109 15.571569 160-169
TTGTC 3845225 2.2241235 14.667234 170-179
TCTGC 3946370 2.210106 12.436583 210-219
AGCTC 3344760 2.2054763 13.919373 140-149
CAGGC 3880565 2.205269 17.412487 140-149
ATTTG 3134235 2.2045138 15.948419 150-159
TACTA 2363540 2.1989498 12.201934 240-247
AGGGA 3570165 2.1960895 6.785645 150-159
CTCTG 3884025 2.1751904 15.008944 180-189
GAATC 2704515 2.1685576 15.90663 190-199
CAGCC 3388885 2.1635773 12.275761 120-129
ACACG 2756700 2.1401744 13.468128 120-129
AATCG 2662615 2.1349611 15.369308 190-199
GTCCT 3801300 2.128862 15.450415 130-139
CACTA 2349185 2.116156 14.278946 240-247
CTCGC 3876705 2.1021135 16.64243 100-109
GGGGC 5646715 2.0908387 53.647392 15-19
GAACT 2606580 2.0900304 8.874924 160-169
GTTCA 3046490 2.0747182 15.488577 140-149
GATAT 2481745 2.0552309 7.434425 180-189
GGGCG 5545780 2.053465 66.84606 20-24
CTCCA 2762245 2.0462024 9.4657345 150-159
AACCA 1912615 2.0285244 15.052048 80-89
TCTCG 3609610 2.0215085 16.973913 100-109
AATAA 1515955 2.0193193 8.393596 80-89
CCTTC 3208260 2.0185237 13.712939 200-209
TTGGA 3319380 2.0121827 15.4059 170-179
CATAG 2500595 2.0050488 8.928818 210-219
GTCAA 2495130 2.0006666 40.431915 70-79
TTGCA 2935485 1.9991217 14.653905 150-159
TGTAG 3264365 1.9788331 6.8528643 210-219
CGTAG 3347255 1.9646144 10.164563 210-219
ATATG 2371200 1.963684 7.686445 180-189
AGTAT 2363945 1.9576762 13.783772 220-229
TCGGC 3960130 1.9114101 8.320385 190-199
TTTGC 3286645 1.9010342 13.003864 150-159
GTCTC 3393825 1.9006613 11.811076 180-189
AATAT 1656360 1.8739206 10.2774935 110-119
CAGAG 2678910 1.8512661 7.435795 140-149
TGCAG 3125955 1.8347261 13.863614 150-159
ATATA 1618510 1.8310989 10.073585 240-247
GCAGA 2634310 1.8204453 15.019908 150-159
CTGTT 3138535 1.8153657 13.308678 140-149
ACGGC 3171465 1.8022978 13.285438 120-129
CAAAA 1394605 1.7986554 20.821733 70-79
CCATC 2426515 1.7975022 14.991733 240-247
GAGTC 3046560 1.7881266 12.252683 210-219
ATTGT 2535335 1.783268 8.64513 170-179
GTCTG 3536650 1.7630248 9.282261 210-219
CTGTG 3520125 1.7547871 6.055024 210-219
TCAGA 2146120 1.7208203 11.168626 130-139
AGTCT 2525875 1.7201694 13.072585 210-219
TGTGC 3443770 1.7167242 8.218323 220-229
CACCA 1939370 1.6914893 10.639852 230-239
TGCTC 3018095 1.6902393 6.739014 150-159
CAAAG 1789925 1.6898125 29.767338 70-79
TTCAG 2459115 1.6747046 8.045216 150-159
ACCAT 1842710 1.6599213 11.65171 45-49
GTGCT 3319295 1.6546731 7.53456 220-229
ATAGT 1979500 1.6393019 7.906336 220-229
GTCAC 2472330 1.6302112 6.602123 130-139
TGACT 2387035 1.6256167 11.2651205 190-199
ATGTA 1954680 1.6187475 7.846334 150-159
AGATC 2017325 1.6175491 8.260092 130-139
GCTCT 2873485 1.6092525 10.695449 130-139
TCTGG 3202640 1.5965203 11.299444 180-189
TTCCA 2080770 1.5919609 10.400193 230-239
GCTCC 2910505 1.5781988 7.5002794 150-159
ATCGG 2654195 1.5578346 9.368387 190-199
CCTCA 2092110 1.5497832 10.1496105 130-139
CCACC 2155200 1.5457957 8.3200445 230-239
ACTTC 2013655 1.5406125 7.906138 220-229
TAGTT 2161785 1.5205257 9.749947 220-229
CTGCT 2712150 1.5188994 6.344269 90-99
GTTGT 2934005 1.5105987 5.3983946 210-219
TGGTT 2922475 1.5046623 18.451876 10-14
TGATA 1810070 1.4989903 6.820634 180-189
CCAGA 1924730 1.4942714 20.494768 60-69
AACTG 1862240 1.4931972 8.077664 160-169
GCTCA 2259320 1.489756 10.775562 140-149
GGAAT 2087175 1.4896748 10.285889 170-179
GAACC 1918605 1.4895163 12.437839 70-79
CCATA 1653195 1.4892054 15.025475 70-79
AATTG 1792735 1.4846345 10.649414 170-179
GCGTA 2508785 1.4724886 10.501637 210-219
TCCCA 1983195 1.4691015 10.386289 240-247
CTGGT 2945625 1.4683981 5.7677913 160-169
GAATT 1741705 1.4423746 11.785918 170-179
AGAAC 1526310 1.4409419 8.788308 160-169
ACTGA 1780475 1.4276359 7.085627 180-189
AGCCG 2497700 1.4194069 10.895941 120-129
ATCAT 1507665 1.4026755 7.0785003 230-239
GTGTT 2714210 1.3974352 8.103947 160-169
GACGT 2376715 1.3949724 7.242822 170-179
CCTTG 2481675 1.3898253 14.839489 20-24
GTAGG 2657925 1.3886164 5.678262 160-169
GGACG 2725720 1.3787926 5.3871207 170-179
GGTCA 2343965 1.3757504 5.640052 130-139
GAGAA 1633510 1.372703 8.0506 160-169
ACGGA 1981720 1.3694717 10.077276 200-209
ATAAC 1246095 1.3649782 8.9792595 80-89
CCCAC 1896690 1.360382 9.630668 240-247
TTGTA 1908830 1.3426057 6.640447 210-219
TCCAC 1810315 1.3410363 9.682166 240-247
GATCT 1968275 1.3404331 6.6827645 130-139
GGCCG 3220385 1.3396193 10.826592 120-129
CCTCG 2463005 1.3355455 8.622171 130-139
GTCCC 2452105 1.3296351 15.867105 50-59
CACTG 2016075 1.3293648 5.4783754 230-239
GAGGG 2888575 1.300626 6.2540374 200-209
TTGGT 2524695 1.2998617 17.000523 25-29
ATATC 1384305 1.2879059 8.141804 70-79
GTGGA 2458440 1.2843969 13.528816 25-29
AATAG 1306780 1.2741722 7.145427 240-247
ATAGA 1297860 1.2654747 8.147042 240-247
CTCTT 1939910 1.2605721 6.9871154 200-209
CTGAT 1835005 1.2496737 6.6907735 180-189
GAAGA 1484375 1.247379 17.0345 35-39
CTTGA 1824175 1.2422982 6.8314238 200-209
AAAGT 1268020 1.2363794 21.878801 70-79
TCACG 1875040 1.2363687 10.117183 200-209
AAATA 927195 1.2350649 6.068512 9
ATACC 1368230 1.2325075 10.246892 70-79
TCTGT 2129025 1.2314532 7.227319 210-219
AAAAG 1069130 1.2273779 14.815165 70-79
AGTTC 1795990 1.2231038 5.273877 160-169
TATGG 2011045 1.21908 5.724129 180-189
AGAGC 1755650 1.2132454 6.302763 140-149
CAGCG 2120045 1.2047912 7.575329 160-169
TGGAC 2046680 1.201264 8.412556 170-179
CTTCT 1838805 1.1948731 6.625582 210-219
AAGTA 1211440 1.1812112 16.907227 70-79
TAACT 1268685 1.1803374 6.653224 240-247
GGAGT 2258485 1.1799315 7.431785 210-219
GCATA 1470580 1.1791531 7.7557106 210-219
GGCTC 2441710 1.1785241 7.558873 130-139
ACCGT 1786440 1.1779474 12.842397 45-49
AAGAG 1391325 1.1691854 13.190524 35-39
CAGAC 1492390 1.1586226 10.664594 60-69
ACTAT 1244630 1.1579576 6.2552185 230-239
GCTTC 2051255 1.1487749 5.132813 150-159
AGCTT 1685225 1.1476706 5.6558566 150-159
GGGAG 2517540 1.1335617 5.2070484 150-159
CTACC 1527450 1.1314969 8.084287 230-239
TTGGC 2266475 1.129841 8.027226 60-69
CTGAA 1392765 1.1167588 14.433009 35-39
AACTT 1192330 1.1092995 6.9181814 200-209
CATCA 1230065 1.108048 8.745684 230-239
CATGT 1611775 1.0976498 7.254117 150-159
CTGCC 2018480 1.0945053 6.5667453 200-209
GCTGA 1864510 1.0943425 11.36422 30-34
ATCCA 1213940 1.0935224 15.2294655 240-247
GGAGG 2426370 1.092511 10.2003 30-34
GTGGT 2457785 1.0905895 7.757231 50-59
CTTCC 1726385 1.0861802 7.6208344 230-239
CGACT 1646635 1.0857624 6.685635 190-199
AAAAT 814660 1.0851631 6.29388 8
TATCA 1163075 1.0820817 7.8057303 70-79
CAATA 984870 1.0788311 6.922213 100-109
TATAA 950165 1.0749677 9.233654 240-247
CCATG 1629225 1.0742826 7.382525 150-159
ACGTG 1821010 1.0688109 7.0060787 170-179
AGCGT 1810285 1.062516 7.4545817 160-169
AGGTT 1751660 1.0618429 23.913084 10-14
ATAAA 796000 1.0603073 6.4289513 5
TTGAG 1733840 1.0510405 6.4347916 200-209
TCATA 1122145 1.044002 5.477757 240-247
ACTCG 1582760 1.0436444 6.8577895 190-199
TCGAC 1579880 1.0417453 7.092646 190-199
CGAAC 1340145 1.0404266 12.800829 70-79
ATGAT 1252615 1.0373399 10.884134 240-247
TGCGT 2075020 1.0344003 8.029798 210-219
CGGCT 2138350 1.0321034 6.6839495 130-139
TGCCC 1901220 1.0309219 5.5840473 200-209
CTCGG 2135220 1.0305927 7.749202 130-139
TTCTG 1766010 1.021481 6.0839033 210-219
CTCGA 1548350 1.020955 6.3563943 190-199
GGGGT 2643095 1.0107863 8.821945 10-14
TAGGC 1717620 1.0081278 6.9419575 160-169
AGAGA 1198300 1.0069789 12.093787 35-39
ACTGG 1715390 1.006819 5.6693854 160-169
TAGAT 1203015 0.99626416 6.413269 240-247
TCCCC 1633595 0.99514574 5.8405633 90-99
TGCTA 1459350 0.9938454 5.0766277 220-229
TCTAC 1295875 0.9914514 6.450781 180-189
CGGAG 1949965 0.9863805 6.9613285 210-219
CCAGC 1541720 0.9842855 6.895646 80-89
GTCCA 1479455 0.9755269 11.822549 70-79
TATGT 1384155 0.9735673 6.096171 220-229
CTGCG 2013160 0.971679 6.637777 210-219
AGCTG 1649310 0.96803445 6.0309763 80-89
ACAAT 883660 0.9679651 8.204655 100-109
AAAGA 838990 0.9631736 5.9007163 8
AGTTT 1367025 0.96151865 5.282908 220-229
TACTG 1407575 0.9585856 5.740993 180-189
TATGA 1156440 0.9576935 8.423432 240-247
TATTT 1172765 0.95711267 6.9188786 220-229
GTCTA 1402785 0.9553235 6.099726 180-189
ACCCA 1094230 0.954371 11.154136 240-247
GAGCT 1614640 0.9476855 5.4510703 140-149
TGGTC 1894545 0.9444333 8.356036 50-59
TGTAA 1120050 0.9275575 6.52515 230-239
TACAA 841000 0.92123526 6.4238462 100-109
ATGGG 1726280 0.9018843 21.09069 15-19
TGCAT 1320080 0.89899987 6.117597 210-219
CCATT 1167735 0.8934138 6.175923 50-59
GGAAG 1452200 0.8932812 5.65214 30-34
GGTGG 2334050 0.8925997 14.384956 25-29
ACTAA 811200 0.88859224 11.553705 240-247
GTATT 1245915 0.876334 6.520958 220-229
ATCAA 793620 0.869335 13.241486 70-79
CCCAA 995050 0.86786765 8.130068 240-247
TTTAT 1056430 0.8621698 6.150933 220-229
TGAGA 1193290 0.8516842 5.251118 240-247
CATAC 940525 0.84722906 13.237969 70-79
ATTTA 880565 0.84612715 5.934765 220-229
CTATA 905550 0.8424901 5.076592 90-99
TCCAG 1260260 0.8309935 5.1837487 240-247
TGAAG 1139035 0.8129609 9.110101 35-39
ACGTC 1230775 0.81155163 7.98224 70-79
CGTGG 1875170 0.805631 5.362513 50-59
TCGAA 981905 0.7873195 15.517906 70-79
CCTCT 1250620 0.78684574 5.098342 100-109
AGCCA 994405 0.7720101 7.364602 80-89
AGCAG 1106100 0.7643726 5.6986628 80-89
CATAT 794870 0.73951757 6.6519322 240-247
AAAGG 879285 0.7388979 8.607226 10-14
AAGGT 1035000 0.73870826 10.150539 10-14
CCAAT 807995 0.72784543 7.911362 240-247
GAAAA 627920 0.720862 6.7457013 7
TAACC 790455 0.7120453 6.3512707 80-89
GAGGT 1358650 0.7098183 7.2503724 10-14
GTAGC 1204510 0.70696664 5.1824036 80-89
CATTG 1036365 0.70578456 5.560506 50-59
CCGTA 1064170 0.7016952 6.5711956 80-89
CTAAT 754055 0.70154476 9.073962 240-247
GACCG 1229565 0.69874406 12.041149 45-49
CCCAT 937890 0.6947657 5.625087 240-247
ATCCC 926585 0.6863911 5.310479 240-247
GCGTG 1593100 0.68444496 5.1789885 160-169
CCGAA 874300 0.67876613 5.156266 90-99
ATAGG 943295 0.67325586 5.134502 10-14
AATCC 742430 0.6687842 7.412113 240-247
AAAGC 697590 0.6585731 9.842117 70-79
GAGGC 1288815 0.651941 7.617611 30-34
CATCC 870540 0.64487445 9.000689 240-247
AATGG 892610 0.63708055 6.5832195 10-14
TAGGT 1048875 0.63582 5.2854543 10-14
CAATC 687235 0.6190643 7.566477 240-247
TGGGC 1436410 0.61712617 11.650146 15-19
GGGCC 1474425 0.6133329 10.824644 15-19
GGGTC 1422555 0.61117357 5.6551275 70-79
GTCGA 1029600 0.6043063 10.894813 70-79
CGTCC 1091210 0.59170014 6.813694 70-79
AAGGG 941235 0.578975 6.3941717 10-14
GGTCC 1184170 0.57155555 8.553287 50-59
TGGGT 1217565 0.5402684 5.234945 10-14
AAGCA 570840 0.5389123 6.336976 70-79
TGATC 780275 0.53138226 6.283144 240-247
AAGAA 460445 0.52859807 5.0231233 8
ATAAG 532565 0.519276 5.8377185 9
TACCG 771045 0.5084136 6.697902 70-79
TAAAT 449140 0.5081339 5.127639 8
TAAAA 381170 0.5077353 7.391115 8
GATCC 731415 0.48228228 6.441843 240-247
TAAGG 675245 0.48194113 5.9947767 10-14
CGGTT 947725 0.47244218 8.419753 10-14
CGATG 789005 0.46309307 10.084148 15-19
GGTCG 1016060 0.43653077 5.670939 70-79
GGAAA 446850 0.37550572 5.045877 6
ATTAA 323770 0.3662967 5.0649056 2
AAGCT 446230 0.35779998 5.175144 30-34
TAAAG 365700 0.35657477 5.2139688 9
AATTA 264405 0.29913422 5.5595202 6