FastQCFastQC Report
Thu 4 May 2017
HCGCLBCXY_s1_1_BC_X.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s1_1_BC_X.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11891411
Filtered Sequences 0
Sequence length 251
%GC 53

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TCCCT 17795185 6.44889 103.89585 30-34
TAATA 11117060 6.2227683 41.185474 110-119
CACAG 13903895 5.980804 34.74399 110-119
ATACA 10673315 5.8916135 28.35629 110-119
CAGTA 13438420 5.861787 35.83785 60-69
CCCTG 17836160 5.822494 72.399254 30-34
GGTGA 16496945 5.19417 90.94674 45-49
GGAGA 14285460 5.127258 80.1872 35-39
AGTAA 10003645 5.0440235 34.5064 110-119
GTAAT 10739910 4.7505198 29.801952 110-119
AATAC 7949930 4.3883195 30.651064 110-119
ACTCC 10621380 4.3877487 113.87357 30-34
GTGAC 12692895 4.3751135 73.433876 45-49
AGACG 11087000 4.356333 83.88972 40-44
GTAGT 12419285 4.3409424 20.533594 80-89
CTCAG 11436360 4.315514 31.057293 140-149
ACAGT 9762210 4.2582383 27.685701 110-119
ACCAG 9800370 4.2156596 78.85957 45-49
CCCCA 10214470 4.161194 86.75416 60-69
CACTC 10005595 4.1333647 93.17763 25-29
CTCCC 11393960 4.071913 70.18191 30-34
GAGAC 10067245 3.955648 118.255 40-44
GGCCC 13313880 3.9150515 57.141098 60-69
TGACC 9998645 3.7729921 121.0241 45-49
ATGCA 8625325 3.7623334 85.52302 25-29
GACCA 8524095 3.666666 88.70424 45-49
CGTGT 11987630 3.624797 18.481163 120-129
GCACA 8382325 3.6056833 27.424667 110-119
GTGTC 11892015 3.5958848 19.34104 120-129
CCCAG 9541990 3.5507898 76.5317 60-69
GACGG 11160310 3.4652145 97.76799 40-44
TGCAC 9150165 3.4528174 110.72487 25-29
AGTAG 8653740 3.4480183 23.508904 60-69
TGAGG 10880280 3.4257267 62.41773 35-39
CCTGA 8990390 3.3925269 81.77348 30-34
AGGAG 9301855 3.33857 81.095055 35-39
CTGAG 9640100 3.3228457 93.504776 35-39
GGATG 10534765 3.3169389 69.503296 20-24
CAGGG 10680970 3.316382 34.669827 50-59
GGGTT 12000540 3.3146303 40.803913 50-59
ATGGT 9467170 3.3090825 20.853325 190-199
ACGGT 9588300 3.3049908 83.12337 40-44
TAGTA 7428970 3.286012 23.277351 80-89
GCACT 8668260 3.2709708 85.92233 25-29
GCCCC 9957650 3.205574 68.370544 60-69
GAGGA 8757940 3.143351 105.84706 35-39
AAAAA 4306315 3.1322477 8.1687355 120-129
TGTCC 9454805 3.1298175 17.430511 120-129
TACAC 6537345 3.1217582 22.565575 110-119
GGTTG 11176305 3.086971 76.25589 15-19
CGGAT 8855325 3.0523417 77.31123 20-24
GATGC 8809330 3.0364876 93.7558 25-29
CCACT 7290715 3.0118337 20.678017 240-247
GTTGG 10784445 2.9787366 54.88725 15-19
CCTGG 9929880 2.9609811 26.975185 50-59
CTCTC 8167075 2.9597092 12.170989 100-109
CAGAT 6769520 2.9528384 14.941545 150-159
TGGTG 10658345 2.9439063 16.058075 190-199
CCAGG 8628335 2.932898 37.706158 50-59
AGGGT 9279310 2.921651 47.47622 50-59
AGGCT 8468855 2.9191294 17.46534 140-149
AGATA 5788410 2.918624 14.763152 150-159
TCAGG 8326075 2.8699145 18.55731 140-149
CCGTG 9482285 2.8275135 19.426785 120-129
TGGCC 9388675 2.7995996 41.428837 60-69
TCTTG 8320020 2.7928617 14.525752 170-179
GCGGA 8879010 2.7568834 90.565834 20-24
CTTGG 9114975 2.7561688 12.630456 170-179
TTCCC 7569820 2.743267 43.870815 50-59
CTTCA 6522170 2.7321947 12.679854 200-209
CCCTT 7484900 2.7124925 15.666327 20-24
GGCGG 11033175 2.7070808 48.54961 20-24
TCTCA 6435110 2.6957245 17.893072 130-139
CGGTG 9807770 2.6714425 64.65679 40-44
TCGCA 7067650 2.6669798 19.077593 100-109
GGTTC 8761695 2.649345 44.97161 50-59
CGCAC 7109535 2.6456184 20.205355 100-109
CCAGT 6872680 2.5934086 34.660645 60-69
TCAAA 4685435 2.5863352 42.296215 70-79
TTGGG 9329695 2.5769248 82.10166 15-19
AGTCA 5893615 2.570772 27.096018 70-79
GCCGT 8556520 2.55146 18.471598 120-129
TGTCT 7519760 2.5242312 15.876483 180-189
TCTCT 6867730 2.523797 12.03793 180-189
GGCTG 9135260 2.488264 12.293278 140-149
ACAGC 5711265 2.4567184 10.675963 120-129
CGGCC 8325150 2.448076 10.703522 120-129
TGGGG 9822220 2.4438195 58.335308 15-19
GTTCC 7345295 2.4315078 49.70422 50-59
CAGCT 6434150 2.4279287 11.437279 150-159
ACCAC 5137720 2.4194107 12.915907 230-239
TCCAT 5765215 2.4150996 13.610648 70-79
GCCCT 7243210 2.3644967 14.008322 200-209
GATGG 7473600 2.3531113 11.672472 190-199
TGTTC 6959220 2.3360693 11.492994 150-159
CTGGC 7816025 2.3306527 33.044033 60-69
TACCA 4868290 2.3247397 13.629341 230-239
TTCTT 6215685 2.316269 15.6344185 170-179
TCCTC 6385325 2.3140113 15.175047 130-139
TCAGC 6097515 2.300899 13.001996 140-149
AGATG 5772465 2.2999957 13.983731 180-189
GGGGC 9068400 2.2250073 55.03049 15-19
AACCA 4072890 2.217067 12.090104 70-79
GCTGT 7212645 2.180946 9.41174 140-149
GGGCG 8869495 2.176204 68.22032 20-24
CACGG 6357360 2.16096 9.17153 120-129
GAGAT 5403405 2.1529465 13.489852 180-189
GACTC 5680755 2.1436348 13.979596 190-199
ACTAC 4473400 2.136169 11.242957 240-247
CTGGA 6051945 2.0860448 11.284799 180-189
CATTT 4900050 2.0815113 14.123432 150-159
TCATT 4868995 2.068319 13.996272 150-159
TGAAT 4654475 2.058786 11.864658 190-199
CAGGC 6021525 2.0468047 15.099951 140-149
TCTGC 6170700 2.0426824 10.468524 210-219
GTTCT 6060960 2.0345416 14.13216 160-169
GTCAA 4662775 2.0338843 32.440907 70-79
TTCAT 4765690 2.024436 13.339542 140-149
TAGTC 5252720 2.0099614 25.282778 70-79
TGGAG 6382115 2.0094502 11.309431 180-189
CTACT 4793140 2.0078888 7.802675 230-239
ACAGA 4030830 2.0042624 8.079269 140-149
ATTTG 5163960 2.003757 12.685807 150-159
TTGTC 5958485 2.0001428 12.035901 170-179
CTCGC 6086790 1.9869913 14.4160385 100-109
CAAAA 3145595 1.9793199 16.03807 70-79
CAGCC 5293685 1.9698997 10.175102 120-129
AGCTC 5209970 1.965984 11.503631 140-149
CTCTG 5875175 1.9448549 12.203757 180-189
ATCTC 4627730 1.9385971 6.56666 100-109
GTCCT 5839400 1.9330122 13.246834 130-139
GATAC 4411165 1.9241331 12.161476 150-159
TGGAA 4826635 1.923137 8.2817545 170-179
AATAA 3009210 1.9201028 6.8072796 80-89
TACAG 4356985 1.9004999 12.134622 160-169
ACACA 3491130 1.9003875 12.129687 120-129
TTCAC 4494530 1.8827984 14.041285 200-209
GAATC 4268970 1.8621082 12.218136 190-199
TCGGC 6236180 1.8595603 7.102629 190-199
ACCAT 3883100 1.8542849 9.979547 45-49
ACTCT 4426360 1.8542415 10.321355 200-209
GTTCA 4835640 1.8503653 12.176849 140-149
TACTA 3811755 1.845787 9.543295 230-239
TTGGA 5254820 1.8367298 12.654903 170-179
GTGAA 4586085 1.8272916 13.516757 190-199
TTTGC 5416285 1.8181373 10.703324 150-159
CCTTC 5010715 1.8158594 11.103323 200-209
TCACA 3801655 1.8153926 7.054531 140-149
TCTCG 5470745 1.8109767 13.638249 100-109
TTGCA 4711790 1.8029739 11.774094 150-159
AGGGA 4985020 1.7891957 5.444199 160-169
CGTAG 5189950 1.7889237 8.970328 80-89
AATCG 4097455 1.787294 11.743096 190-199
ACGGC 5224635 1.7759302 11.9389 120-129
TGTAG 5039225 1.7613723 5.5718784 150-159
CACTA 3681020 1.7577863 11.107537 240-247
AATAT 3120555 1.7467289 7.7157493 110-119
CTGTT 5195475 1.7440157 10.933662 140-149
CATAG 3996385 1.7432077 6.712798 210-219
GCAGA 4420410 1.7368789 12.100819 150-159
CCATA 3556305 1.6982316 15.870892 70-79
ACACG 3934310 1.6923556 11.690136 120-129
GTCTC 5101775 1.6888368 9.821946 180-189
AGTAT 3809615 1.6850841 10.3726225 220-229
GATAT 3808950 1.68479 5.8105044 180-189
TGCTC 5070955 1.6786345 6.1263237 150-159
TGCAG 4829575 1.6647061 11.578478 150-159
CACCA 3517505 1.656433 8.96763 230-239
CTGTG 5433845 1.6430757 5.322619 210-219
CTCCA 3974075 1.6417116 7.6281834 150-159
ATATG 3690760 1.6325119 5.8662615 180-189
ATTGT 4182515 1.6229297 6.8146915 170-179
TGGTT 5282650 1.6197982 17.50655 10-14
GAGTC 4684410 1.6146691 9.748312 210-219
GTGCT 5308320 1.6051198 6.550761 220-229
CTGCT 4818245 1.5949801 5.8233657 140-149
CAGAG 4057530 1.5942951 6.124653 140-149
TGTGC 5264150 1.5917639 7.2891283 220-229
TCAGA 3637125 1.5864997 8.615367 130-139
GCTCT 4739705 1.568981 9.234455 130-139
GAACT 3577195 1.5603586 6.625274 160-169
TCTGG 5145595 1.5559152 9.799723 180-189
CAAAG 3120195 1.5514646 22.511358 70-79
CCAGA 3599270 1.5482373 20.201208 60-69
CTGGT 5080245 1.536155 5.231301 160-169
AAAAT 2372370 1.513751 5.121324 90-99
TTCAG 3954955 1.5133697 6.9743104 150-159
GCTCC 4606775 1.5038503 6.927884 150-159
GTCTG 4955805 1.4985269 7.769457 210-219
CCATC 3593895 1.4846574 11.54131 240-247
ATAGT 3353815 1.483473 6.3489695 220-229
AGTCT 3852645 1.4742206 10.117363 210-219
GCTCA 3893580 1.4692436 9.30028 140-149
CCTCA 3517815 1.4532284 8.151271 130-139
ATATA 2584850 1.4468684 7.0452423 240-247
CCACC 3527135 1.4368922 7.1727557 230-239
TGACT 3730175 1.4273573 9.055189 190-199
ATAAC 2580750 1.4245603 7.392295 80-89
ACTTC 3387055 1.4188673 6.286991 210-219
ATGTA 3204015 1.4172128 6.3444266 160-169
GACGT 4060790 1.3997136 6.6816387 60-69
ATACC 2928905 1.398631 11.589267 70-79
GTCCC 4261290 1.3910692 17.109287 50-59
ACCGT 3669935 1.3848512 15.093811 45-49
GTCAC 3657830 1.3802832 5.5546217 140-149
TGATA 3115985 1.378275 5.490625 180-189
CCTTG 4128725 1.3667289 10.343288 20-24
GAACC 3171105 1.3640606 10.409128 70-79
GGCCG 5075220 1.3632379 10.202849 120-129
GCGTA 3942890 1.3590746 8.767879 210-219
ACTGA 3062865 1.3360099 5.730607 180-189
AGCCG 3929615 1.3357339 9.154498 120-129
GAGAA 2928905 1.3302993 6.57395 160-169
TTGGT 4308760 1.3211783 11.202464 25-29
CTGAT 3448660 1.319635 5.849295 180-189
CCTCG 4033270 1.3166337 7.632917 130-139
TAGTT 3383845 1.313024 8.101579 220-229
AACTG 3003940 1.310307 6.4242306 160-169
ATCAT 2697705 1.3063244 5.342203 230-239
GTGTT 4241995 1.3007063 6.7743735 160-169
GTAGG 4118845 1.296845 5.4950438 160-169
CAATA 2341920 1.2927275 5.6081185 100-109
AGAAC 2585990 1.2858402 6.819922 160-169
AAAAG 2226745 1.2798754 11.372219 70-79
TCCCA 3091760 1.2772225 8.387776 240-247
AATAG 2532320 1.2768428 6.04079 240-247
AATTG 2881845 1.2747092 8.017122 170-179
ATCGG 3693725 1.2731899 7.4189696 190-199
CATCA 2666015 1.273094 6.636317 230-239
CTCTT 3426370 1.2591443 5.9624014 200-209
TTGGC 4100315 1.2398456 8.0381365 60-69
CTTCT 3361560 1.2353275 5.8650303 210-219
CAGCG 3630125 1.2339327 6.7252192 160-169
ACGGA 3120535 1.2261287 7.8050466 200-209
TCTGT 3632010 1.2191921 6.2629795 210-219
TTCCA 2907305 1.2178959 7.3751283 230-239
TCCAC 2930180 1.210473 7.5646834 240-247
TCACG 3202465 1.2084512 8.141429 200-209
GAATT 2731705 1.2082987 8.628293 170-179
TTCTG 3558995 1.1946826 5.5530925 210-219
AGATC 2737720 1.1941829 6.4867373 130-139
TGGTC 3945530 1.1930419 10.18953 50-59
CTTGA 3111740 1.1907121 5.7582817 200-209
TTGTA 3066895 1.1900389 5.4873376 220-229
GCTGA 3434500 1.1838377 9.242019 30-34
GAGGG 4167830 1.1820825 5.328528 200-209
TATCA 2435315 1.179266 6.03282 70-79
CCCAC 2887880 1.176471 8.384556 240-247
GGAAT 2949185 1.1750808 7.7072535 170-179
GGCTC 3931650 1.1723747 6.8800426 130-139
AAAGT 2297000 1.1581903 15.786276 70-79
GTGGT 4192690 1.1580492 9.200922 50-59
GATCT 3014785 1.1536121 5.421963 130-139
CAGAC 2663530 1.1457257 11.677131 60-69
ATATC 2363005 1.1442508 6.2465005 70-79
AGGTT 3252755 1.1369432 21.87694 10-14
CATAC 2371575 1.1324909 14.310605 70-79
GCATA 2593860 1.1314317 6.3030014 220-229
GGAGT 3584785 1.1286927 6.1520987 210-219
TGGAC 3247635 1.1194272 7.1659546 170-179
CTGCC 3423080 1.1174412 6.0883284 200-209
CCAGC 2999650 1.1162376 6.1217885 80-89
AAGAG 2440035 1.108256 10.049553 35-39
TGCCC 3390315 1.1067452 5.3395762 60-69
ATAGA 2186965 1.1027085 6.555554 240-247
CCATG 2917870 1.1010594 6.2824383 150-159
AGCGT 3173285 1.0937995 6.4088807 160-169
AGAGC 2779795 1.0922443 5.258898 140-149
GAAGA 2393170 1.0869701 13.647413 35-39
ATCCA 2250625 1.0747341 11.402553 240-247
GTCCA 2833625 1.0692693 13.153893 70-79
CTACC 2576985 1.0645663 6.8556166 230-239
TGCGT 3519025 1.0640762 7.1498218 210-219
AAGTA 2107305 1.0625423 12.1542015 70-79
CGGCT 3563195 1.0625055 6.2219067 130-139
AACTT 2180115 1.055689 5.269726 210-219
TTGAG 3004290 1.0500966 5.366693 200-209
CGAAC 2422210 1.0419211 10.744396 70-79
TCCCC 2904105 1.0378536 5.1508756 90-99
CTCGG 3479585 1.0375739 7.079846 130-139
GTGGA 3287080 1.0349584 9.65936 25-29
GGAGG 3615690 1.0254842 7.516835 30-34
ACGTG 2944520 1.0149465 5.861044 170-179
TATAA 1807580 1.011792 6.656838 240-247
AGCTG 2926010 1.0085663 5.176844 90-99
CTTCC 2752255 0.9974041 5.907425 230-239
GGGGT 4007895 0.99718523 8.591851 10-14
CTGAA 2285930 0.99711376 11.745074 35-39
TATTT 2313940 0.99675655 5.359213 220-229
CTGCG 3320000 0.9899875 5.8276205 210-219
ATCAA 1788140 0.9870438 10.01454 70-79
CGACT 2611115 0.98530513 5.4551716 200-209
AGAGA 2161920 0.9819371 9.634675 35-39
TAGGC 2843025 0.9799622 6.388818 160-169
CGGAG 3127205 0.9709798 6.0546684 210-219
ACAAT 1749160 0.96552706 6.26909 100-109
TAACC 2016255 0.962816 5.6191363 80-89
CATGT 2508660 0.95994264 6.0145063 150-159
TATGT 2456150 0.95305306 5.2670984 220-229
GTCTA 2469980 0.9451416 5.1416163 180-189
CGTGG 3443455 0.937929 6.6535807 50-59
CCATT 2238850 0.9378742 5.0123596 50-59
ATGAT 2108025 0.93243 7.9878373 240-247
AAAGC 1864245 0.9269646 8.237464 70-79
TCTAC 2204615 0.9235327 5.1546555 180-189
ACTCG 2426430 0.9156142 5.5277543 190-199
GTATT 2316085 0.89870405 5.0872207 220-229
CTCGA 2366560 0.8930221 5.096844 190-199
AAGCA 1794360 0.8922154 5.5799937 70-79
TCGAC 2363545 0.89188445 5.6039906 190-199
AAAGG 1919075 0.87163776 7.57466 10-14
TGCAT 2261185 0.86524594 5.1615276 210-219
ACTAA 1558145 0.8600878 8.499224 240-247
AGCCA 1998940 0.8598503 6.1102133 80-89
TGTAA 1913270 0.84628534 5.355003 230-239
TAGAT 1880810 0.83192736 5.284541 240-247
TGAAG 2077525 0.8277744 7.7885494 35-39
AAGGT 2041730 0.81351215 9.150259 10-14
CCGTA 2133130 0.8049374 6.7720037 80-89
CGTCC 2439755 0.79644144 8.088321 70-79
ACCCA 1679660 0.7909709 8.896556 240-247
ACGTC 2089195 0.78835845 9.011362 70-79
CCAAT 1648360 0.7871364 6.2866435 240-247
TATGA 1777365 0.78617126 6.2400775 240-247
ATGGG 2487430 0.78318346 15.110467 15-19
GGTGG 3135955 0.780242 10.718149 25-29
CAATC 1610400 0.76900935 6.041543 240-247
TCGAA 1744460 0.76092666 12.664432 70-79
CCCAA 1610600 0.75844985 6.359802 240-247
CTAAT 1560925 0.75585514 6.823397 240-247
GACCG 2187665 0.74361944 14.56174 45-49
GAGGC 2340150 0.7266036 5.8753324 30-34
AATCC 1520045 0.7258624 5.901347 240-247
GAAGG 2006300 0.72009003 5.179245 10-14
TACCG 1884330 0.7110526 8.28404 70-79
CATCC 1696065 0.70065355 7.124221 240-247
AATGG 1742755 0.6943878 5.910746 10-14
GGTCC 2273855 0.6780386 10.612102 50-59
GGGTC 2411830 0.65693474 5.1843195 70-79
GAGGT 2084030 0.6561703 6.8852067 10-14
TAGGT 1820530 0.6363342 5.209371 10-14
CGGTT 2011450 0.6082185 8.37481 10-14
GTCGA 1672385 0.57645434 9.517547 70-79
GGTCG 2091470 0.5696751 5.238419 70-79
GGGCC 2051045 0.55092436 8.59654 15-19
TGGGC 1987620 0.5413883 9.188435 15-19
AAGGG 1501685 0.53897643 6.0603156 10-14
TAAGG 1268275 0.5053348 5.2079988 10-14
TGGGT 1749095 0.48311183 5.3267684 10-14
TGATC 1235305 0.47269136 5.0676813 240-247
CGATG 1285845 0.44321787 6.354678 15-19
GATCC 1097655 0.41420048 5.1752963 240-247