FastQCFastQC Report
Thu 4 May 2017
HCGCLBCXY_s1_1_illumina12index_7_SL248371.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s1_1_illumina12index_7_SL248371.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2509802
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 2964355 8.921425 59.59824 110-119
ATACA 3003405 8.630999 39.085197 110-119
CACAG 3885690 7.781927 44.238277 110-119
CAGTA 3500610 7.342089 42.716686 60-69
TCCCT 4786555 7.2494993 116.46601 30-34
AGTAA 2687255 7.085838 49.0291 110-119
GGAGA 3881465 6.544629 101.38905 35-39
GTAAT 2944140 6.466827 40.081356 110-119
GGTGA 4488730 6.3046846 109.36523 45-49
CCCTG 4606300 6.1124516 79.48416 30-34
AATAC 2095445 6.0217595 42.272263 110-119
GTAGT 3554930 5.6989217 33.83157 80-89
ACAGT 2697495 5.657656 36.35033 110-119
AGACG 3055460 5.614754 105.15863 40-44
CTCAG 3153490 5.260912 38.68559 140-149
GTGAC 3382665 5.17802 87.82248 45-49
ACTCC 2818195 5.1239567 132.48079 30-34
TAGTA 2286040 5.021305 42.77695 80-89
GAGAC 2677140 4.919549 147.6113 40-44
CCCCA 2744130 4.764115 98.97981 60-69
CACTC 2571150 4.6747866 109.18942 25-29
ACCAG 2331910 4.6701493 90.38128 45-49
GCACA 2295380 4.5969906 34.893158 110-119
ATGCA 2190515 4.59433 109.43851 25-29
AGTAG 2319375 4.463567 32.620472 80-89
CGTGT 3396995 4.3316183 21.453386 120-129
TGACC 2589955 4.320776 144.4702 45-49
CTCCC 2979075 4.308335 75.197876 30-34
GGCCC 3662025 4.2575793 59.393288 60-69
AGATA 1609920 4.245087 22.004143 150-159
GTGTC 3263440 4.161318 22.171268 120-129
TACAC 1780640 4.070211 28.7552 110-119
CCCAG 2553790 4.0681586 88.50907 60-69
CAGAT 1900955 3.987014 20.562235 150-159
GACCA 1980385 3.9661453 104.132965 45-49
AGGAG 2343100 3.9507556 102.51075 35-39
ATGGT 2463010 3.94846 27.176363 190-199
TGCAC 2358390 3.9344602 130.62434 25-29
TGAGG 2776700 3.900038 73.26148 35-39
GACGG 2865160 3.8426588 114.34967 40-44
ACGGT 2494145 3.8179164 100.54815 40-44
GAGGA 2257255 3.8060105 132.53725 35-39
CTGAG 2478600 3.7941208 109.42102 35-39
CCTGA 2273055 3.7920978 97.86708 30-34
GGATG 2698840 3.790679 83.95763 20-24
GCACT 2206465 3.6810064 101.564735 25-29
TGTCC 2642555 3.6723504 20.05516 120-129
AGGCT 2318800 3.5495067 22.08694 140-149
CGGAT 2316355 3.545764 92.87507 20-24
CAGGG 2630185 3.527518 36.818665 50-59
GGGTT 2980545 3.487274 42.428543 50-59
CTCTC 2274450 3.444779 13.616889 130-139
CCCTT 2272500 3.4418256 20.567284 50-59
TCAGG 2227445 3.4096649 23.08944 140-149
CCACT 1874545 3.4082408 26.425684 240-247
CCGTG 2791365 3.3987172 21.88414 120-129
GCCCC 2678455 3.393834 72.204155 60-69
GATGC 2216705 3.3932247 111.30388 25-29
CTTCA 1770020 3.3703072 16.958345 200-209
TCTCA 1753350 3.3385656 22.720522 130-139
GGTTG 2781315 3.2541728 85.559845 15-19
CTTGG 2517060 3.209585 15.170648 170-179
TCTTG 2195205 3.194862 17.95784 170-179
TGGTG 2730435 3.1946425 18.896492 190-199
TCGCA 1911175 3.1883793 22.5089 110-119
ACAGC 1584620 3.173541 13.734446 120-129
CGCAC 1968000 3.1350012 23.232067 100-109
GTTGG 2671590 3.1257932 61.40704 15-19
CCAGG 2119545 3.0980647 39.767857 50-59
CCTGG 2523120 3.0721066 27.399626 50-59
AGGGT 2177820 3.0588758 52.297005 50-59
GCGGA 2268130 3.0419416 104.98259 20-24
TCCAT 1572575 2.994351 22.432909 70-79
TGTCT 2003255 2.915501 19.551256 180-189
AGATG 1514475 2.9145613 19.456806 180-189
TCTCT 1836980 2.9137096 15.124213 180-189
TGGCC 2390890 2.9111054 44.061054 60-69
AGTCA 1379775 2.8939044 31.127417 70-79
CGGTG 2587525 2.8908 72.16942 40-44
GCCGT 2299680 2.8000498 20.544762 120-129
ACCAC 1282610 2.799487 17.270096 230-239
CCAGT 1671190 2.7880166 38.603474 60-69
CAGCT 1666590 2.7803426 13.52338 150-159
TGTTC 1905625 2.7734122 14.706623 150-159
TCAAA 964440 2.771548 55.377827 70-79
GGCTG 2463825 2.7526014 14.242079 140-149
TTCCC 1816155 2.7506657 44.580753 50-59
GATGG 1958075 2.7502313 15.260193 190-199
TTGGG 2341195 2.7392268 92.766 15-19
GAGAT 1421205 2.7350662 18.577597 180-189
CGGCC 2342925 2.7239542 11.24481 120-129
GGTTC 2135185 2.7226434 46.46735 50-59
ACTAC 1188510 2.7167125 16.512356 240-247
TCCTC 1782840 2.7002087 18.195517 130-139
GGCGG 2747625 2.6894853 51.270943 20-24
TTCTT 1608150 2.671316 20.319506 170-179
GCCCT 2011645 2.6694052 16.873219 200-209
TACCA 1134635 2.5935638 18.360561 230-239
CACGG 1753605 2.5631828 10.423674 120-129
CTGGA 1670365 2.5569139 14.737157 180-189
TCATT 1265020 2.522584 19.026785 150-159
TACAG 1198780 2.5142903 17.389042 160-169
GATAC 1197850 2.5123396 16.707254 150-159
CATTT 1255355 2.503311 19.167362 150-159
TGGGG 2440895 2.5021753 63.702015 15-19
GTGAA 1287825 2.4783804 19.251484 190-199
ACACA 902885 2.4775515 16.691618 120-129
GCTGT 1937840 2.4710023 11.429891 140-149
TCAGC 1479585 2.4683654 15.167326 140-149
ATTTG 1347470 2.4654856 17.303862 150-159
TGAAT 1119765 2.4595728 16.76508 190-199
TTCAT 1226385 2.4455414 17.538202 140-149
GTTCC 1759055 2.4445531 50.91921 50-59
ACAGA 957700 2.41132 10.247999 140-149
TTGTC 1652605 2.4051716 14.621538 170-179
CGTAG 1568970 2.4017034 13.5091095 80-89
GACTC 1435910 2.3955033 16.476683 190-199
CAGGC 1638120 2.3943825 18.462349 140-149
AATCG 1130230 2.3705153 16.58601 190-199
TTCAC 1244770 2.370175 18.936543 200-209
TGGAG 1686380 2.3686197 14.452276 180-189
GTTCT 1618870 2.356074 17.183977 160-169
GAATC 1121590 2.3523939 17.048975 190-199
TGGAA 1210585 2.3297343 11.389654 170-179
TACTA 972235 2.3273888 14.08396 240-247
CTACT 1198225 2.2815483 10.113203 230-239
AGCTC 1355770 2.261807 13.639173 140-149
GGGGC 2304310 2.2555509 58.74728 15-19
CTCTG 1604965 2.230415 15.034463 180-189
GTCCT 1599385 2.2226603 16.083107 130-139
CAGCC 1392410 2.2180932 12.104898 120-129
GTTCA 1269155 2.2173848 15.485257 140-149
CTGGC 1819385 2.215251 32.847713 60-69
TAGTC 1261710 2.2043774 28.178162 70-79
GGGCG 2248820 2.201235 71.66248 20-24
TTTGC 1500565 2.1838953 13.334053 150-159
ATCTC 1141140 2.1728525 8.0976515 100-109
AGGGA 1287770 2.171339 6.885029 160-169
AGTAT 987955 2.170051 15.568509 220-229
TTGCA 1240635 2.1675568 15.403129 150-159
TCTGC 1552135 2.1569974 12.809536 210-219
ACTCT 1129010 2.1497557 12.666676 200-209
CATAG 1012470 2.1235285 9.167097 210-219
TCACA 927355 2.1197605 8.502924 140-149
CTCGC 1596490 2.1185045 15.401548 100-109
TTGGA 1306760 2.094872 15.797768 170-179
AATAA 579565 2.0938988 8.975393 80-89
TCGGC 1712020 2.0845253 8.453842 190-199
CCAGA 1038350 2.0795183 32.163193 60-69
ACACG 1038225 2.079268 14.417973 120-129
AACCA 755790 2.0739172 14.110414 80-89
CACTA 897355 2.051186 15.492764 240-247
GATAT 933395 2.0502095 7.2035108 180-189
CCATA 893855 2.0431857 27.165731 70-79
ATATG 921175 2.0233684 7.5274496 180-189
GCAGA 1097275 2.0163674 16.13592 150-159
CCTTC 1322905 2.003612 13.882003 200-209
AAAAA 460205 1.9959713 12.682098 120-129
TCTCG 1425080 1.9804294 15.605376 100-109
AATAT 653265 1.966045 10.856388 110-119
GTCTC 1411750 1.9619048 12.334544 180-189
GAACT 934565 1.9601325 8.35602 160-169
CTGTT 1338855 1.9485457 13.676284 140-149
TGTAG 1213630 1.9455749 6.6632643 150-159
TGCAG 1268470 1.9417125 14.3927 150-159
GAGTC 1262845 1.933102 13.289097 210-219
ATTGT 1056130 1.9324164 8.947726 50-59
ACCGT 1155600 1.9278669 24.301065 45-49
ACGGC 1308415 1.9124643 13.641736 120-129
CAGAG 1037720 1.9069282 7.3756485 140-149
GTCAA 895585 1.8783768 36.88118 70-79
ATATA 618110 1.8602434 10.33206 240-247
CAAAA 532810 1.8380992 24.003712 70-79
CTCCA 1003515 1.8245605 8.820015 150-159
AGTCT 1038875 1.8150549 14.0096035 210-219
ACCAT 788210 1.8017008 13.439598 45-49
ATAGT 809340 1.7777218 8.942302 220-229
ATGTA 808815 1.7765687 8.2909565 160-169
CTGTG 1388685 1.7707573 6.033104 210-219
GACGT 1154500 1.7672527 11.023725 60-69
ATACC 766180 1.7513446 19.93439 70-79
CACCA 797200 1.7400076 11.3733835 230-239
TCAGA 826620 1.7337315 10.743875 130-139
TGTGC 1341750 1.7109088 8.688195 220-229
TCTGG 1337780 1.7058467 12.013155 180-189
GCTCT 1225035 1.7024273 11.142923 130-139
TGCTC 1218665 1.693575 6.588065 150-159
GTCTG 1328155 1.6935735 9.753329 210-219
AGATC 801045 1.6800911 8.494112 130-139
GTCCC 1262250 1.6749759 23.849615 50-59
GTGCT 1288930 1.6435562 7.4331203 220-229
AATTG 731620 1.6070092 11.297039 170-179
CAGAC 795370 1.5928988 19.814373 60-69
ATCGG 1031145 1.5784266 9.672502 190-199
GCGTA 1025810 1.5702603 11.203451 220-229
GAACC 782385 1.5668936 11.454541 70-79
GGAAT 808350 1.5556452 10.6807575 170-179
CAAAG 617840 1.5556123 26.782377 70-79
GCTCC 1171535 1.5545992 7.534636 150-159
TGACT 884655 1.5456117 10.728439 190-199
TTCAG 884325 1.545035 7.827113 150-159
GAATT 702055 1.5420694 12.318287 170-179
GTTGT 1154485 1.541702 5.3462224 210-219
AGCCG 1045215 1.5277541 10.9695425 120-129
TGGTT 1139340 1.5214773 20.430706 10-14
CCTCA 832980 1.514499 9.743083 130-139
TAGTT 823455 1.5066876 10.192353 220-229
GCTCA 893325 1.4903183 10.406612 140-149
GTCAC 893220 1.4901431 6.3577185 140-149
CCATC 811690 1.4757901 14.489426 240-247
ATCAT 611340 1.4634588 6.9445143 240-247
ACTTC 768380 1.4630775 7.84812 220-229
TGATA 662865 1.4559884 6.7470765 180-189
ACGGA 792270 1.4558861 10.5349865 200-209
GTAGG 1031265 1.4484721 6.5533586 160-169
CCTCG 1090355 1.4468753 8.8775835 130-139
AACTG 689525 1.4461919 8.059882 160-169
CCACC 830955 1.4426302 8.518391 230-239
CCTTG 1033340 1.4360294 11.63973 20-24
AGAAC 566700 1.4268508 8.905763 160-169
CTGCT 1023175 1.421903 5.996497 140-149
CTGGT 1114840 1.4215685 5.413477 160-169
GTGTT 1062160 1.418411 8.227089 160-169
GGCCG 1317205 1.4051734 10.867713 120-129
ATAAC 486580 1.3983033 8.999603 80-89
TTGGC 1091990 1.3924317 10.881608 60-69
GAGAA 599540 1.3850924 8.480841 160-169
GATCT 788695 1.3779566 6.7508807 130-139
ACTGA 650270 1.3638595 6.8668256 180-189
GGACG 1012280 1.3576368 5.304146 170-179
TCCCA 745455 1.3553637 10.163218 240-247
GGTCA 876530 1.3417497 5.114123 130-139
GTCCA 799085 1.3330994 21.023987 70-79
CATAC 580830 1.3276691 25.046997 70-79
AATAG 496660 1.3096086 8.766432 240-247
TTGTA 711970 1.302702 6.844888 220-229
CACTG 767380 1.2802064 5.5076323 230-239
CAGCG 873240 1.2763842 8.276272 160-169
TCACG 760205 1.2682366 10.221541 200-209
ATATC 527730 1.2633085 8.657737 70-79
CCCAC 727355 1.2627691 10.0714035 240-247
GTGGT 1072395 1.2547152 14.398749 50-59
GGCTC 1022750 1.2452823 8.102014 130-139
GGAGT 881835 1.2385889 8.112357 210-219
TGGTC 971265 1.2384914 15.7953615 50-59
CTGAT 708275 1.237452 6.7711787 180-189
TATAC 516345 1.2360544 5.350078 110-119
TATGG 764065 1.2248753 5.5855174 190-199
GCATA 581720 1.2200845 8.490386 220-229
ATAGA 462100 1.2184798 8.812505 240-247
TAACT 508765 1.2179092 6.615026 240-247
TTCCA 636165 1.2113261 9.388608 230-239
TCTGT 823085 1.1979032 7.2798705 210-219
TCCAC 653915 1.1889285 8.619149 240-247
AAAAG 372605 1.1794525 17.659706 70-79
TGCCC 887410 1.177572 7.3647714 60-69
AGCGT 762255 1.166823 8.1990385 160-169
GAAGA 504340 1.1651556 20.683826 35-39
AGGTT 726640 1.1648793 27.038195 10-14
AAATA 319830 1.1555073 5.4450126 9
TGGAC 754195 1.1544852 8.249035 170-179
AGCTT 658325 1.1501826 5.4743686 150-159
GAGGG 932235 1.1472116 5.901776 200-209
CGGCT 933515 1.1366314 7.226675 130-139
TTGGT 843925 1.1269795 12.827872 25-29
CTCGG 925215 1.1265255 8.215044 130-139
CTCTT 709035 1.1246296 6.420523 200-209
CTACC 618240 1.1240653 8.863502 230-239
CTTGA 638390 1.1153536 6.498287 200-209
AAGTA 420835 1.1096709 15.177144 70-79
CATGT 631915 1.1040407 7.2004504 150-159
AAAGT 417770 1.101589 19.02305 70-79
GCTGA 719445 1.1012915 10.858636 30-34
AGAGC 596695 1.0964947 6.1176877 140-149
TATCA 456765 1.0934286 8.429841 70-79
TGCGT 857090 1.0929031 8.66669 210-219
AACTT 454825 1.0887848 6.9696455 210-219
ACGTC 651990 1.0877033 14.5302305 70-79
CGAAC 543045 1.087564 11.754183 70-79
CGTGG 966180 1.0794226 9.961233 50-59
CTGCC 811800 1.0772394 6.5942397 200-209
ACTAT 448080 1.0726383 5.935312 230-239
TAGGC 700210 1.0718474 7.598578 160-169
CCGTA 639850 1.0674505 10.227565 70-79
CTTCT 671155 1.0645466 6.520839 210-219
AAGAG 459605 1.0618063 15.778668 35-39
CCATG 636305 1.0615364 7.27543 150-159
TATAA 352565 1.0610679 10.043336 240-247
GTGGA 754630 1.0599221 11.687386 25-29
CAATA 366435 1.0530381 6.629761 100-109
ATAAA 290250 1.0486383 5.262206 7
CGGAG 781085 1.0475657 7.8387074 210-219
ACGTG 684045 1.0471029 6.774042 170-179
AGTGA 543740 1.0464113 5.1166244 160-169
AGTTC 598225 1.0451798 5.2894373 160-169
CATCA 450565 1.0299076 8.450836 230-239
TTGAG 638965 1.0243273 6.421473 200-209
CTGAA 487230 1.0219036 15.810947 35-39
AGAGA 436720 1.008936 14.66816 35-39
CGACT 604590 1.0086268 6.3569703 200-209
ATCCA 439955 1.0056549 14.725624 240-247
TCCCC 694535 1.0044358 5.864524 90-99
TATGT 548905 1.0043395 7.079407 220-229
AAAAT 276260 0.9980942 6.1135917 90-99
CTGCG 816890 0.9946308 6.923409 210-219
TCGAC 592765 0.98889935 6.583851 190-199
TGCTA 565850 0.9886164 5.1708016 230-239
AGCTG 641120 0.9813954 5.982564 90-99
TCATA 409155 0.97945744 5.5559278 240-247
GAGCT 633080 0.9690882 5.3858776 140-149
TATTT 461575 0.9639341 7.064138 220-229
GACCG 659355 0.9637561 22.18102 45-49
ACTCG 576810 0.962282 6.180441 190-199
TGTAA 437355 0.96065384 7.375714 230-239
ACTGG 626395 0.95885515 5.5182834 160-169
AGTTT 523445 0.957755 5.884535 220-229
TTCTG 651420 0.9480648 6.115832 210-219
ATGAT 431455 0.94769436 11.098441 240-247
CTCGA 567685 0.9470589 5.886936 190-199
ACAAT 327585 0.9413934 7.875015 100-109
TCTAC 493365 0.9394196 6.0726447 180-189
TAGAT 427325 0.9386228 6.8394465 240-247
CCATT 489040 0.9311844 6.6475463 50-59
CCAGC 582760 0.92832994 6.4499545 80-89
CTTCC 612200 0.9272104 7.0775466 230-239
TACTG 529595 0.92527395 5.464883 180-189
TACAA 321730 0.92456764 6.1699214 100-109
GTCTA 527400 0.92143905 5.9572086 180-189
GTATT 500780 0.91628456 6.6170774 220-229
GGGGT 888630 0.91093963 8.689501 10-14
ACTAA 314645 0.9042073 12.991696 240-247
ATCAA 312955 0.8993506 14.978656 70-79
TGCAT 512520 0.8954416 6.501208 210-219
CGTCC 669380 0.88825136 11.8848295 70-79
ACCCA 406865 0.8880434 11.939743 240-247
GGAGG 712235 0.87647885 8.85491 30-34
TACCG 520930 0.8690583 13.435994 70-79
GAGTT 541440 0.86798453 5.1108174 160-169
TGAAG 449455 0.8649625 10.449287 35-39
TATGA 393495 0.8643149 8.938349 240-247
GGTCC 688735 0.8385916 15.517518 50-59
CCCCC 598410 0.8263613 5.095717 80-89
TTTAT 394015 0.8228447 6.1318045 220-229
ATTTA 323600 0.8112656 5.9381776 220-229
TGAGA 413365 0.79550844 5.508293 240-247
AAAGA 251295 0.79545504 6.53628 8
AGCCA 392140 0.78534436 6.971751 80-89
CCCAA 348285 0.76018393 7.720933 240-247
TCGAA 362085 0.7594277 14.800454 70-79
AAAGG 324120 0.748801 9.608255 10-14
GCGTG 668380 0.7467185 5.6408796 160-169
ATGGG 531360 0.7463262 17.401968 15-19
CATTG 426350 0.74489105 6.1970124 50-59
AAAGC 293035 0.7378106 11.585709 70-79
AAGGT 382130 0.7353976 11.457353 10-14
CCGAA 366385 0.7337646 6.0379906 90-99
GTAGC 479320 0.7337198 5.3069654 80-89
TATTA 287335 0.7203492 5.2410073 230-239
TAACC 313610 0.716854 6.040751 80-89
CTAAT 294130 0.70410436 9.802188 240-247
CATAT 290640 0.6957498 6.5424204 240-247
AGCAG 374095 0.687442 5.146323 80-89
TAGGG 478420 0.6719689 5.1558504 10-14
TGTTA 365985 0.6696481 5.1243405 230-239
ATCCC 360345 0.6551683 5.327514 240-247
ATAGG 339850 0.65403104 5.603711 10-14
CCCAT 357940 0.65079564 5.5022416 240-247
CCAAT 283625 0.6483138 7.674416 240-247
TAGGT 395925 0.6347088 6.3543615 10-14
AATCC 267160 0.6106779 7.223401 240-247
AATGG 311810 0.60006887 7.0281715 10-14
GGTGG 581655 0.5962578 11.850286 25-29
AGAAA 187160 0.59244055 5.6248612 7
GAGGC 437265 0.5864455 6.910189 30-34
GAGGT 409370 0.5749842 8.220934 10-14
CATCC 313635 0.57024163 8.217848 240-247
AAGCA 225605 0.5680337 7.661222 70-79
GAAGG 336750 0.56780195 6.108571 10-14
GTCGA 369680 0.5658882 9.977675 70-79
CAATC 237035 0.5418178 7.1397543 240-247
AAGGG 318890 0.53768784 6.928004 10-14
TGATC 293995 0.5136489 6.4329104 240-247
GAAAA 158845 0.5028116 6.501958 8
ATAAG 190470 0.50223726 5.9054055 9
GGGCC 469605 0.5009672 8.845651 15-19
AGAGG 286440 0.48297313 5.46505 10-14
TGGGC 426145 0.476092 9.645183 15-19
CGGTT 370520 0.47246203 9.3820915 10-14
TTGCC 326685 0.4539931 5.3166223 60-69
TAAGG 234575 0.45143247 6.4680324 10-14
GATCC 269090 0.44891807 6.3848476 240-247
AAATG 164995 0.43506396 5.3145475 9
AGAAG 185900 0.429477 5.3883266 9
TAAAA 114130 0.412338 6.0543666 8
AAGAA 129965 0.41139418 6.1625595 9
TGGGT 332825 0.3894093 5.6426835 10-14
CGATG 245785 0.37623578 7.3131022 15-19
TAAAG 141010 0.37181956 5.7211595 9