FastQCFastQC Report
Thu 4 May 2017
HCGCLBCXY_s2_1_illumina12index_7_SL248371.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s2_1_illumina12index_7_SL248371.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2691748
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 3180220 8.9546585 59.600597 110-119
ATACA 3221635 8.654216 39.11195 110-119
CACAG 4161265 7.776796 44.337196 110-119
CAGTA 3758465 7.3625174 42.961807 60-69
TCCCT 5139795 7.2556777 116.573616 30-34
AGTAA 2885170 7.114435 49.03232 110-119
GGAGA 4162990 6.5556526 101.34278 35-39
GTAAT 3154600 6.477398 40.151672 110-119
GGTGA 4809940 6.307213 109.30285 45-49
CCCTG 4947755 6.1167045 79.095436 30-34
AATAC 2249360 6.042413 42.25711 110-119
GTAGT 3800395 5.6904864 33.684883 80-89
ACAGT 2896375 5.6737556 36.50088 110-119
AGACG 3276100 5.620178 105.321846 40-44
CTCAG 3383270 5.2650104 38.706673 140-149
GTGAC 3632325 5.1887755 87.88984 45-49
ACTCC 3022915 5.124729 132.6821 30-34
TAGTA 2439930 5.009953 42.536102 80-89
GAGAC 2871225 4.9256115 147.84027 40-44
CCCCA 2949810 4.77088 98.97529 60-69
ACCAG 2504245 4.6800675 90.631065 45-49
CACTC 2756515 4.6731024 108.772484 25-29
ATGCA 2351095 4.6055975 109.762024 25-29
GCACA 2460480 4.598277 34.956924 110-119
AGTAG 2482580 4.464128 32.339 80-89
CGTGT 3643905 4.3344545 21.372795 120-129
TGACC 2780275 4.326636 144.72061 45-49
CTCCC 3201975 4.312307 75.31184 30-34
GGCCC 3933505 4.2585936 59.40622 60-69
AGATA 1726325 4.2568817 21.964848 150-159
GTGTC 3499940 4.1632075 22.137665 120-129
CCCAG 2741885 4.070721 88.45172 60-69
TACAC 1906920 4.0694027 28.735067 110-119
CAGAT 2036365 3.989068 20.47819 150-159
GACCA 2125335 3.97194 104.27516 45-49
AGGAG 2509580 3.9519515 102.45581 35-39
ATGGT 2637650 3.949461 27.245598 190-199
TGCAC 2530400 3.9377828 130.79303 25-29
TGAGG 2976620 3.903204 73.52428 35-39
GACGG 3078860 3.8516545 114.44712 40-44
ACGGT 2674225 3.82013 100.33614 40-44
GAGGA 2422125 3.814232 133.00089 35-39
CTGAG 2661470 3.801909 109.55449 35-39
CCTGA 2440810 3.7983637 97.48234 30-34
GGATG 2893380 3.7940521 83.716125 20-24
GCACT 2370410 3.6888075 101.23837 25-29
TGTCC 2830785 3.6682312 20.008074 120-129
AGGCT 2490480 3.5576503 22.185253 140-149
CGGAT 2486080 3.551365 92.64811 20-24
CAGGG 2826005 3.5353327 36.8931 50-59
GGGTT 3203545 3.4979684 42.581345 50-59
CTCTC 2439490 3.4437468 13.510915 130-139
CCCTT 2434395 3.4365544 20.46873 50-59
TCAGG 2397870 3.4253566 23.227554 140-149
CCACT 2012480 3.4117448 26.465431 240-247
GATGC 2380595 3.400679 111.45311 25-29
CCGTG 2990225 3.393367 21.801619 120-129
GCCCC 2874855 3.3906646 72.12855 60-69
CTTCA 1895325 3.3679767 16.913204 200-209
TCTCA 1880570 3.341757 22.667582 130-139
GGTTG 2986215 3.2606647 85.74025 15-19
CTTGG 2704285 3.2167695 15.233148 170-179
TCTTG 2357595 3.202281 18.072464 170-179
TGGTG 2925910 3.1948175 18.927404 190-199
TCGCA 2050915 3.1916127 22.613028 110-119
ACAGC 1698935 3.1750612 13.7668915 120-129
CGCAC 2115135 3.1402209 23.301231 100-109
GTTGG 2866980 3.1304715 61.57177 15-19
CCAGG 2281460 3.1092284 39.85991 50-59
CCTGG 2711930 3.0775526 27.44277 50-59
AGGGT 2336745 3.0641437 52.54516 50-59
GCGGA 2435685 3.0470421 105.16413 20-24
TCCAT 1683545 2.9916458 22.373928 70-79
TGTCT 2152510 2.923718 19.594753 180-189
TGGCC 2572760 2.9196198 44.00996 60-69
TCTCT 1970050 2.9150755 15.187813 180-189
AGATG 1621105 2.9150403 19.462059 180-189
CGGTG 2780480 2.8964388 71.94557 40-44
AGTCA 1478145 2.8955615 31.18403 70-79
ACCAC 1379630 2.808797 17.356585 230-239
GCCGT 2463470 2.795595 20.470467 120-129
TCAAA 1038905 2.7907906 55.654934 70-79
CCAGT 1791040 2.7871983 38.688885 60-69
CAGCT 1788220 2.7828097 13.498633 150-159
TGTTC 2045990 2.7790337 14.840751 150-159
GGCTG 2649000 2.7594757 14.2704935 140-149
TTCCC 1954350 2.7588909 44.823765 50-59
GATGG 2094740 2.7468057 15.247564 190-199
TTGGG 2512610 2.7435327 92.93371 15-19
GAGAT 1521360 2.7356808 18.579657 180-189
GGTTC 2294870 2.7297668 46.62176 50-59
CGGCC 2515585 2.723488 11.273719 120-129
ACTAC 1267585 2.70505 16.445362 240-247
TCCTC 1913990 2.701916 18.250208 130-139
GGCGG 2960310 2.700592 51.322803 20-24
TTCTT 1725360 2.6760411 20.37962 170-179
GCCCT 2155615 2.6648977 16.943214 200-209
TACCA 1219465 2.602361 18.51997 230-239
CACGG 1879295 2.5611484 10.407844 120-129
CTGGA 1789615 2.5564644 14.683996 180-189
TCATT 1357055 2.527687 19.085245 150-159
TACAG 1286970 2.521066 17.502993 160-169
GATAC 1284725 2.516668 16.67071 150-159
CATTT 1347880 2.5105977 19.31254 150-159
TGGGG 2622570 2.5077796 63.50659 15-19
ACACA 967550 2.4796157 16.65839 120-129
GTGAA 1376830 2.4757898 19.193476 190-199
ATTTG 1446645 2.473463 17.391224 150-159
GCTGT 2079380 2.4734395 11.42026 140-149
TCAGC 1581305 2.460811 15.212092 140-149
TTCAT 1318455 2.4557896 17.545748 140-149
TGAAT 1194735 2.4531713 16.694773 190-199
GTTCC 1892160 2.451928 51.04224 50-59
TTGTC 1774325 2.4100356 14.628697 170-179
CAGGC 1763800 2.4037488 18.53551 140-149
ACAGA 1021435 2.40292 10.308835 140-149
CGTAG 1678825 2.398201 13.551553 80-89
GACTC 1540485 2.3972871 16.447645 190-199
TGGAG 1805970 2.3681455 14.366584 180-189
TTCAC 1330655 2.364563 18.903473 200-209
AATCG 1205710 2.3618844 16.457907 190-199
GTTCT 1734375 2.3557723 17.111475 160-169
GAATC 1199180 2.3490927 16.911684 190-199
TGGAA 1301510 2.3403506 11.4328 170-179
TACTA 1033520 2.3118386 13.983272 240-247
CTACT 1276435 2.2682145 9.986355 230-239
GGGGC 2477710 2.260332 58.52707 15-19
AGCTC 1451670 2.2590742 13.63142 140-149
CTCTG 1721960 2.2313764 15.087444 180-189
CTGGC 1960420 2.2247238 32.834435 60-69
GTTCA 1363335 2.223851 15.503287 140-149
GTCCT 1715640 2.223187 16.090397 130-139
CAGCC 1488785 2.2103148 12.108034 120-129
TAGTC 1353570 2.207922 28.247631 70-79
GGGCG 2414025 2.2022345 71.69587 20-24
TTTGC 1608425 2.1846967 13.402802 150-159
TTGCA 1332205 2.1730719 15.4242 150-159
AGGGA 1379425 2.1722443 6.9301987 160-169
AGTAT 1056860 2.17007 15.641035 220-229
ATCTC 1220935 2.1695914 7.990453 100-109
ACTCT 1210090 2.1503203 12.635353 200-209
TCTGC 1658180 2.1487281 12.811659 210-219
CATAG 1085700 2.1267953 9.1232195 220-229
CTCGC 1713230 2.1179955 15.396569 100-109
TCACA 990590 2.1139374 8.498114 140-149
TTGGA 1409050 2.109828 15.927651 170-179
AATAA 622670 2.1055355 9.124225 80-89
TCGGC 1836290 2.0838585 8.422266 190-199
AACCA 810380 2.076824 14.0697565 80-89
ACACG 1110030 2.0744839 14.410753 120-129
CCAGA 1107320 2.0694194 31.972778 60-69
CACTA 968205 2.0661674 15.675649 240-247
GATAT 997135 2.0474355 7.135109 180-189
CCATA 955980 2.040079 27.085894 70-79
ATATG 985445 2.0234323 7.4687467 180-189
GCAGA 1179005 2.0225935 16.10606 150-159
CCTTC 1418640 2.002647 13.857603 200-209
AATAT 702505 1.9780682 10.919867 110-119
TCTCG 1525745 1.977114 15.538386 100-109
GTCTC 1515090 1.963307 12.377179 180-189
GAACT 1000035 1.9589846 8.404122 160-169
CTGTT 1436300 1.9509016 13.662738 140-149
TGTAG 1302175 1.9498 6.6958895 150-159
TGCAG 1363825 1.9482238 14.43705 150-159
ATTGT 1134995 1.9406062 8.977891 180-189
GAGTC 1352560 1.9321315 13.318556 210-219
ACCGT 1236150 1.9236841 24.014635 45-49
ACGGC 1406445 1.9167368 13.678902 120-129
AAAAA 469430 1.9062867 11.346728 120-129
CAGAG 1110995 1.9059216 7.3407187 140-149
GTCAA 964280 1.8889436 37.052757 70-79
ATATA 663640 1.8686347 10.274317 240-247
CAAAA 572095 1.8455776 24.0293 70-79
CTCCA 1073745 1.8203132 8.826754 150-159
AGTCT 1113180 1.8158016 14.052594 210-219
ACCAT 842900 1.7987642 13.326117 45-49
ATAGT 868200 1.7826909 8.979662 220-229
ATGTA 866190 1.7785637 8.293807 160-169
CTGTG 1484130 1.7653848 6.0383825 210-219
GACGT 1235180 1.7644545 10.898484 60-69
CACCA 855320 1.741351 11.3614025 230-239
ATACC 814980 1.7391825 19.895088 70-79
TCAGA 883660 1.7310156 10.6609125 130-139
TGTGC 1437070 1.7094066 8.6808 220-229
TCTGG 1436195 1.7083658 11.962164 180-189
GCTCT 1315130 1.7041918 11.035465 130-139
TGCTC 1305775 1.6920693 6.5692306 150-159
GTCTG 1418365 1.6871569 9.751822 210-219
AGATC 852225 1.669437 8.463039 130-139
GTCCC 1350255 1.6692644 23.744543 50-59
GTGCT 1387325 1.6502346 7.454683 220-229
AATTG 784520 1.6108693 11.318445 170-179
CAGAC 843880 1.5770884 19.659723 60-69
GAACC 841825 1.5732478 11.406048 70-79
ATCGG 1099115 1.5700854 9.611122 190-199
GCGTA 1094050 1.5628501 11.21313 220-229
CAAAG 663765 1.5615033 26.95793 70-79
GGAAT 867240 1.5594546 10.707751 170-179
GCTCC 1258540 1.5558809 7.532827 150-159
GAATT 755505 1.5512922 12.408855 170-179
TGACT 949995 1.5496169 10.750153 190-199
TTCAG 948955 1.5479203 7.818893 150-159
GTTGT 1232855 1.5371631 5.3117995 210-219
AGCCG 1117375 1.5227852 10.901595 120-129
TGGTT 1220790 1.5221201 20.362974 10-14
CCTCA 895290 1.5177795 9.763482 130-139
TAGTT 882250 1.5084647 10.14657 220-229
GTCAC 955935 1.4876163 6.3630195 140-149
GCTCA 954135 1.484815 10.299909 140-149
CCATC 870195 1.4752362 14.586365 240-247
ACTTC 824275 1.46473 7.7980666 220-229
TGATA 711700 1.4613466 6.6547327 180-189
ATCAT 652410 1.4593493 6.9570317 240-247
ACGGA 847955 1.4546742 10.504822 200-209
AACTG 742240 1.4539859 8.00007 160-169
GTAGG 1107775 1.4526113 6.564913 160-169
CCACC 894755 1.4471337 8.535631 230-239
CCTCG 1168115 1.4440922 8.89078 130-139
CCTTG 1108515 1.4364527 11.81121 20-24
AGAAC 609280 1.4333277 8.967055 160-169
CTGGT 1201145 1.4287717 5.3807707 160-169
GTGTT 1140180 1.421613 8.240795 160-169
CTGCT 1096670 1.4211036 5.97977 140-149
ATAAC 524875 1.4099617 9.174812 80-89
GGCCG 1417655 1.4088821 10.836423 120-129
TTGGC 1168405 1.3898274 10.87036 60-69
GAGAA 643535 1.3896914 8.532698 160-169
GATCT 845510 1.3791826 6.7770553 130-139
GGACG 1092210 1.366355 5.265735 170-179
ACTGA 696935 1.3652371 6.786193 180-189
TCCCA 798730 1.354082 10.225013 240-247
GGTCA 941785 1.3453395 5.096213 130-139
GTCCA 853270 1.3278501 20.98307 70-79
CATAC 618900 1.3207442 25.012262 70-79
TTGTA 764575 1.3072648 6.918248 220-229
AATAG 529495 1.3056624 8.712082 240-247
CACTG 822245 1.2795693 5.381001 230-239
CAGCG 933265 1.271876 8.358748 160-169
TCACG 813580 1.2660849 10.18969 200-209
CCCAC 781535 1.2640171 10.200994 240-247
ATATC 564915 1.2636353 8.709898 70-79
GTGGT 1146500 1.2518698 14.358345 50-59
GGCTC 1098170 1.2462252 8.069821 130-139
CTGAT 761780 1.2426034 6.685467 180-189
GGAGT 942970 1.2365044 8.186886 210-219
TGGTC 1039440 1.2364225 15.728739 50-59
TATAC 550450 1.231279 5.287576 110-119
TATGG 819795 1.2275125 5.5969343 190-199
ATAGA 497300 1.2262739 8.990735 240-247
TAACT 546775 1.2230586 6.6776104 240-247
GCATA 622310 1.2190529 8.5096855 220-229
TTCCA 680615 1.2094471 9.328057 230-239
TCCAC 706470 1.1976742 8.682313 240-247
TCTGT 881340 1.1971091 7.289465 210-219
AAAAG 399530 1.1831266 17.736376 70-79
TGCCC 948945 1.1731415 7.2822824 60-69
AGCGT 817685 1.1680629 8.246242 160-169
AGGTT 778555 1.1657621 26.9991 10-14
TGGAC 815060 1.1643131 8.253935 170-179
AAATA 343685 1.162158 5.372663 9
AGCTT 708540 1.1557593 5.4771214 150-159
GAAGA 533335 1.1517183 20.49656 35-39
CGGCT 1005560 1.1411296 7.231628 130-139
GAGGG 990455 1.1373909 5.9021487 200-209
CTACC 665340 1.1279467 8.859496 230-239
TTGGT 901275 1.1237384 12.729072 25-29
CTCTT 759370 1.1236371 6.4079666 200-209
CTCGG 988985 1.12232 8.235007 130-139
AAGTA 453055 1.1171718 15.370286 70-79
CTTGA 680805 1.1105185 6.463528 200-209
GCTGA 773895 1.1055088 10.948124 30-34
AAAGT 447515 1.1035109 19.154724 70-79
CATGT 675885 1.102493 7.238901 150-159
TATCA 492785 1.1022905 8.466052 70-79
AGAGC 636680 1.0922301 6.063805 140-149
CGAAC 583110 1.0897473 11.738684 70-79
TGCGT 915495 1.0889889 8.656207 210-219
AACTT 484120 1.0829083 6.977898 210-219
ACGTC 694425 1.0806571 14.521602 70-79
CGTGG 1034575 1.0777216 9.9477625 50-59
ACTAT 480740 1.0753478 5.948572 230-239
TAGGC 752015 1.0742533 7.6074533 160-169
TATAA 380940 1.0726261 10.0660715 240-247
CTGCC 867380 1.0723059 6.5929184 200-209
CCGTA 685090 1.0661299 10.161348 70-79
CCATG 681245 1.0601466 7.290317 150-159
GTGGA 807655 1.0590677 11.839355 25-29
CTTCT 715480 1.058693 6.504333 210-219
CAATA 393685 1.0575485 6.70278 100-109
AAGAG 488355 1.0545856 15.607787 35-39
CGGAG 836960 1.0470371 7.871288 210-219
ACGTG 731980 1.0456333 6.713749 170-179
ATAAA 308805 1.0442126 5.128451 7
AGTTC 639115 1.0425143 5.2323627 160-169
AGTGA 579170 1.0414525 5.0829587 160-169
CATCA 481940 1.028469 8.388677 230-239
TTGAG 685050 1.0257534 6.344432 200-209
CTGAA 517185 1.0131218 15.753652 35-39
TCCCC 749595 1.009528 5.9273705 90-99
CGACT 647900 1.0082552 6.4015727 200-209
ATCCA 471535 1.0062644 14.787655 240-247
AGAGA 464435 1.0029311 14.576901 35-39
TATGT 585080 1.0003655 7.081102 220-229
AAAAT 295325 0.9986305 6.063001 90-99
TGCTA 609680 0.9945004 5.1994076 230-239
CTGCG 873690 0.9914809 6.904463 210-219
TCGAC 634855 0.9879548 6.518039 190-199
TCATA 439310 0.98267454 5.5887933 240-247
AGCTG 686280 0.9803508 6.002761 90-99
GAGCT 677985 0.96850145 5.306365 140-149
ACTGG 675515 0.9649731 5.50323 160-169
TATTT 493620 0.96373814 7.0970163 220-229
ACTCG 618245 0.9621066 6.130443 190-199
AGTTT 561305 0.9597152 5.8856435 220-229
GACCG 701645 0.9562184 21.925077 45-49
TGTAA 465100 0.9549984 7.3496346 230-239
ATGAT 464705 0.9541873 11.302035 240-247
ACAAT 353090 0.94849885 7.962192 100-109
CTCGA 608800 0.9474084 5.851483 190-199
TAGAT 458720 0.94189817 6.926013 240-247
TTCTG 693300 0.94169754 6.1231427 210-219
TCTAC 527590 0.93752307 6.06417 180-189
TACAA 346455 0.9306754 6.30519 100-109
CCATT 523465 0.930193 6.621507 50-59
CTTCC 656040 0.92610985 7.1413875 230-239
GTCTA 566335 0.9237967 5.96252 180-189
TACTG 565835 0.9229812 5.382118 180-189
CCAGC 621360 0.92249805 6.4446907 80-89
GTATT 536635 0.9175347 6.668465 220-229
GGGGT 955085 0.91328067 8.786001 10-14
ATCAA 338050 0.9080972 15.036087 70-79
ACTAA 335370 0.90089804 12.956673 240-247
TGCAT 547890 0.8937096 6.5166917 210-219
CGTCC 718365 0.8880849 11.883365 70-79
ACCCA 435510 0.88665736 11.860685 240-247
GGAGG 757995 0.870445 8.822614 30-34
TATGA 421125 0.8647036 9.022468 240-247
TACCG 555290 0.86413664 13.413101 70-79
GAGTT 577075 0.86407787 5.0270905 160-169
TGAAG 477585 0.85878426 10.393182 35-39
CCCCC 654065 0.8403731 5.214478 80-89
GGTCC 734810 0.8338771 15.42249 50-59
TTTAT 420425 0.8208331 6.1528516 220-229
ATTTA 345365 0.809762 5.9493203 220-229
TGAGA 443645 0.79775405 5.5768967 240-247
AAAGA 266750 0.7899257 6.027489 8
AGCCA 419930 0.7847877 6.9739785 80-89
CCCAA 374110 0.76165277 7.6521306 240-247
TCGAA 387420 0.7589231 14.812362 70-79
AAAGG 348810 0.753243 9.700087 10-14
GCGTG 719295 0.74929297 5.677846 160-169
ATGGG 569740 0.74709284 17.516754 15-19
CATTG 454890 0.7420095 6.1773787 50-59
AAAGC 315125 0.7413297 11.662252 70-79
GTAGC 517850 0.73974866 5.324508 80-89
AAGGT 410265 0.7377308 11.600071 10-14
CCGAA 393705 0.735777 6.2030506 90-99
TATTA 310315 0.7275818 5.3384194 230-239
TAACC 337025 0.7192176 6.0964565 80-89
CTAAT 312585 0.6992086 9.763456 240-247
CATAT 311485 0.69674814 6.547708 240-247
AGCAG 398565 0.68374175 5.114768 80-89
TAGGG 516665 0.67749625 5.3086905 10-14
TGTTA 388595 0.66441697 5.091422 230-239
ATAGG 366715 0.65941995 5.6322913 10-14
ATCCC 386280 0.65485805 5.371119 240-247
CCCAT 385320 0.6532306 5.54895 240-247
CCCGA 435310 0.64628005 5.125697 90-99
CCAAT 302645 0.64585006 7.6261535 240-247
TAGGT 424770 0.6360254 6.3365936 10-14
AATCC 287135 0.6127514 7.2649536 240-247
AATGG 334820 0.602067 7.1703386 10-14
AGAAA 202365 0.59926265 5.3395495 7
GGTGG 619380 0.59226954 11.906347 25-29
GAGGC 469985 0.5879513 6.931818 30-34
AAGCA 244010 0.57403207 7.7247167 70-79
CATCC 337170 0.5716022 8.282053 240-247
GAGGT 435665 0.5712819 8.104931 10-14
GAAGG 359565 0.5662236 6.1474547 10-14
GTCGA 393175 0.56165045 9.93549 70-79
CAATC 254850 0.54385465 7.1865706 240-247
AAGGG 343460 0.5408623 6.9928436 10-14
GATGA 296410 0.53299886 5.107122 240-247
TGATC 313100 0.51072377 6.4842267 240-247
ATAAG 206320 0.50875694 5.8382735 9
GGGCC 503995 0.5008761 8.957141 15-19
GAAAA 166230 0.49225622 6.6013656 8
AGAGG 301750 0.47517967 5.358931 10-14
TGGGC 455435 0.4744288 9.760195 15-19
CGGTT 396340 0.47144967 9.344416 10-14
TAAGG 253165 0.4552365 6.48378 10-14
TTGCC 347410 0.45018613 5.3095317 60-69
GATCC 286575 0.44596505 6.4649577 240-247
AAATG 177030 0.4365318 5.232779 9
AGAAG 197190 0.42582494 5.5859065 9
TAAAA 121715 0.41157475 6.1867027 8
AAGAA 138640 0.41055405 6.027489 9
TGGGT 354110 0.3866547 5.617339 10-14
CGATG 266380 0.38052374 7.3286676 15-19
TAAAG 150020 0.36992884 5.752198 9