FastQCFastQC Report
Thu 4 May 2017
HCGCLBCXY_s2_1_illumina12index_1_SL248356.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s2_1_illumina12index_1_SL248356.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2033332
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 2411980 8.981235 59.6213 110-119
ATACA 2431565 8.651151 38.893486 110-119
CACAG 3145585 7.7888556 44.02424 110-119
CAGTA 2843550 7.3689814 43.023228 60-69
TCCCT 3876975 7.259161 117.192986 30-34
AGTAA 2182985 7.114106 48.883427 110-119
GGAGA 3152640 6.5495253 101.75479 35-39
GTAAT 2385670 6.4704027 40.028267 110-119
GGTGA 3643105 6.29881 109.11277 45-49
CCCTG 3739310 6.127624 79.590965 30-34
AATAC 1699430 6.046323 42.122593 110-119
ACAGT 2199590 5.700177 36.114086 110-119
GTAGT 2856755 5.6435523 33.22478 80-89
AGACG 2476025 5.615764 105.942154 40-44
CTCAG 2553235 5.2615585 38.43481 140-149
GTGAC 2751465 5.1936088 88.50925 45-49
ACTCC 2273480 5.114853 132.37743 30-34
TAGTA 1833935 4.9739895 42.01522 80-89
GAGAC 2174720 4.9323874 148.21889 40-44
CCCCA 2230125 4.7939878 99.757484 60-69
CACTC 2070350 4.657853 109.3795 25-29
GCACA 1859665 4.6047597 35.02622 110-119
ATGCA 1771045 4.5896144 109.94448 25-29
ACCAG 1843810 4.5655003 91.55671 45-49
AGTAG 1868510 4.435306 32.07562 80-89
TGACC 2107035 4.3420553 146.25864 45-49
CGTGT 2761050 4.337414 21.251888 120-129
CTCCC 2418310 4.3264437 75.78649 30-34
GGCCC 2991570 4.290492 59.857086 60-69
AGATA 1305955 4.2559624 21.961344 150-159
GTGTC 2655600 4.171759 22.075642 120-129
TACAC 1446460 4.0923376 28.60347 110-119
CCCAG 2072110 4.080018 89.04412 60-69
GACCA 1615820 4.000969 105.3904 45-49
AGGAG 1902805 3.9530263 102.90773 35-39
ATGGT 1998850 3.9487505 26.810953 190-199
TGCAC 1903985 3.923622 131.08638 25-29
TGAGG 2260945 3.9091003 73.707275 35-39
GACGG 2331245 3.851238 114.87835 40-44
CAGAT 1482795 3.8426228 20.579624 150-159
ACGGT 2025265 3.8228486 100.81463 40-44
GAGGA 1836310 3.814885 133.73264 35-39
CTGAG 2013510 3.8006601 109.580765 35-39
CCTGA 1840495 3.7927854 98.071945 30-34
GGATG 2178005 3.7656999 83.64584 20-24
GCACT 1781940 3.6721187 101.61116 25-29
TGTCC 2138055 3.6668556 19.860573 120-129
AGGCT 1884235 3.5566432 21.71296 140-149
CAGGG 2150830 3.553191 37.00732 50-59
CGGAT 1871675 3.5329354 92.75672 20-24
GGGTT 2415210 3.4753063 42.559235 50-59
CCCTT 1842560 3.4499676 20.620234 50-59
CTCTC 1842430 3.4497247 13.559348 130-139
TCAGG 1807400 3.411611 22.8632 140-149
GCCCC 2174595 3.4049027 72.6203 60-69
CCGTG 2265385 3.4003549 21.706757 120-129
CCACT 1509290 3.395586 26.715406 240-247
GATGC 1792350 3.383203 110.78479 25-29
CTTCA 1435685 3.3804584 17.00481 200-209
TCTCA 1417130 3.3367689 22.602524 130-139
GGTTG 2249640 3.2370632 84.901985 15-19
CTTGG 2051910 3.2234051 15.122715 170-179
TGGTG 2238860 3.2215514 18.707344 190-199
TCTTG 1786175 3.2060726 17.919521 170-179
TCGCA 1551570 3.1973855 22.725811 110-119
ACAGC 1274590 3.156042 13.637589 120-129
CGCAC 1601165 3.15272 23.141266 100-109
CCAGG 1738345 3.1352115 40.06254 50-59
GTTGG 2163045 3.1124594 61.34634 15-19
CCTGG 2051620 3.0794926 27.532154 50-59
AGGGT 1776125 3.070862 52.772823 50-59
GCGGA 1826900 3.0180554 105.06686 20-24
TCCAT 1273730 2.9991198 22.312382 70-79
TGTCT 1633325 2.931716 19.527718 180-189
TCTCT 1495495 2.9305744 15.07196 180-189
TGGCC 1944535 2.9187574 44.194416 60-69
AGATG 1224635 2.9069319 19.306015 180-189
CGGTG 2100185 2.887496 72.15921 40-44
AGTCA 1108920 2.873736 31.168365 70-79
ACCAC 1041395 2.8151796 17.02688 230-239
GCCGT 1866315 2.8013492 20.40689 120-129
CAGCT 1346885 2.7755823 13.333716 150-159
TTCCC 1479835 2.77081 45.194183 50-59
CCAGT 1343475 2.7685554 38.627136 60-69
TCAAA 778150 2.7685432 55.297462 70-79
TGTTC 1541875 2.7675688 14.697297 150-159
GATGG 1594065 2.7560866 15.110662 190-199
GGCTG 2004420 2.7558308 14.062668 140-149
GAGAT 1158160 2.7491393 18.616207 180-189
CGGCC 1909900 2.7391672 11.134045 120-129
GGTTC 1739710 2.732961 46.68619 50-59
TTGGG 1890870 2.7208204 92.530106 15-19
ACTAC 961055 2.7190254 16.199324 240-247
TCCTC 1444150 2.703994 17.91007 130-139
GCCCT 1641245 2.6895156 16.644852 200-209
TTCTT 1306535 2.6795578 20.35364 170-179
GGCGG 2225560 2.677999 51.262226 20-24
TACCA 919710 2.6020517 18.196203 230-239
CACGG 1428615 2.576594 10.300319 120-129
CTGGA 1352825 2.5535648 14.681394 180-189
TCATT 1026650 2.5299568 18.951982 150-159
TACAG 970880 2.5160086 17.111729 160-169
GATAC 969445 2.5122902 16.677969 150-159
CATTT 1015385 2.5021968 19.002586 150-159
TGGGG 1970460 2.481493 63.260063 15-19
GTGAA 1040445 2.4697177 19.083376 190-199
ATTTG 1092050 2.464988 17.174618 150-159
GCTGT 1568650 2.464238 11.336393 140-149
GTTCC 1432750 2.4572275 51.222713 50-59
TCAGC 1190705 2.4537358 15.009629 140-149
TGAAT 903350 2.4500616 16.731878 190-199
ACACA 718110 2.441207 16.56572 120-129
TTCAT 990080 2.4398382 17.46635 140-149
ACAGA 775725 2.4154775 10.238121 140-149
GACTC 1171275 2.4136953 16.29596 190-199
TTGTC 1340060 2.405324 14.585724 170-179
CAGGC 1326990 2.3933077 18.220633 140-149
TGGAG 1376595 2.3800879 14.461275 180-189
AATCG 916340 2.37467 16.540705 190-199
CGTAG 1254705 2.3683553 13.2744465 80-89
GTTCT 1315200 2.3607016 17.08463 160-169
TTCAC 1001040 2.3570447 18.850014 200-209
GAATC 907705 2.3522928 17.01945 190-199
TACTA 787645 2.332222 13.996967 240-247
TGGAA 980730 2.3279715 11.217606 170-179
CTACT 968865 2.2812855 10.014566 230-239
AGCTC 1098750 2.26424 13.55504 140-149
TCACA 798210 2.2583027 8.604857 140-149
GGGGC 1867125 2.2466967 58.47078 15-19
CTCTG 1301630 2.2323515 14.922908 180-189
CTGGC 1480825 2.2227266 32.922607 60-69
GTCCT 1290130 2.2126284 15.86216 130-139
CAGCC 1122520 2.2102597 11.970687 120-129
GTTCA 1022250 2.2047281 15.404162 140-149
TAGTC 1018760 2.197201 28.018358 70-79
GGGCG 1813975 2.1827416 71.121124 20-24
TTTGC 1213925 2.1789196 13.209428 150-159
ATCTC 923315 2.1740334 7.986918 100-109
AGGGA 1042060 2.1648517 6.9835334 160-169
ACTCT 919240 2.1644387 12.793059 200-209
TTGCA 1001880 2.160796 15.323779 150-159
AATAA 481140 2.152695 9.149986 80-89
AGTAT 793105 2.1510556 15.350462 220-229
TCTGC 1252845 2.148683 12.814747 210-219
CTCGC 1300415 2.130996 15.467152 100-109
CATAG 814015 2.1094973 9.007296 210-219
AACCA 619325 2.1053889 14.145547 80-89
TTGGA 1062015 2.098023 15.777117 170-179
ACACG 841895 2.0846357 14.380014 120-129
TCGGC 1378500 2.0691361 8.369906 190-199
CACTA 727890 2.0593529 15.369788 240-247
CCATA 725505 2.0526052 26.9755 70-79
GATAT 754450 2.0462155 7.1594605 180-189
ATATG 752615 2.0412388 7.419014 180-189
GCAGA 890335 2.01933 16.017326 150-159
CCTTC 1066525 1.9969375 13.795132 200-209
AATAT 535420 1.9936868 10.903712 110-119
TCTCG 1158645 1.9871261 15.61019 100-109
GTCTC 1146150 1.9656965 12.183012 180-189
TGTAG 990805 1.9573464 6.645761 150-159
GAACT 754260 1.9546442 8.329928 160-169
CTGTT 1084350 1.9463404 13.552852 140-149
CCAGA 782480 1.9375169 31.267023 60-69
TGCAG 1025385 1.9354956 14.282079 150-159
ATTGT 856335 1.9329294 8.979305 50-59
ACGGC 1070985 1.931587 13.519883 120-129
CAGAG 847845 1.9229602 7.1888933 140-149
GAGTC 1013780 1.9135903 13.139227 210-219
ACCGT 926530 1.9093391 24.191114 45-49
GTCAA 725450 1.8799838 36.941303 70-79
AAAAA 346525 1.8629199 10.895614 120-129
ATATA 498515 1.8562676 10.137031 240-247
CAAAA 428445 1.8316044 23.828348 70-79
ACCAT 645515 1.8262967 13.680453 45-49
AGTCT 834285 1.7993367 13.902784 210-219
CTCCA 799750 1.7992698 8.76154 150-159
ATAGT 658730 1.7866043 8.929244 220-229
CTGTG 1130460 1.7758725 5.9574447 210-219
ATGTA 653005 1.7710769 8.311359 160-169
GACGT 934505 1.7639525 10.921183 60-69
TCAGA 673575 1.7455512 10.859639 130-139
ATACC 610050 1.7259588 20.0285 70-79
TGTGC 1091830 1.7151875 8.586672 220-229
TGCTC 994100 1.7049242 6.6654525 150-159
GCTCT 990365 1.6985185 10.963911 130-139
TCTGG 1078880 1.694844 11.869048 180-189
GTCTG 1065175 1.6733143 9.636912 210-219
GTCCC 1015485 1.6640799 23.683226 50-59
GTGCT 1046230 1.6435531 7.3591514 220-229
AATTG 591380 1.6039381 11.128693 170-179
CACCA 590605 1.5965692 10.97759 230-239
GAACC 641925 1.5894854 11.803131 70-79
CAGAC 637685 1.5789866 19.770493 60-69
GCTCC 953615 1.5626932 7.3306284 150-159
CAAAG 501620 1.5619606 26.801733 70-79
ATCGG 824625 1.5565453 9.583888 190-199
GCGTA 822595 1.5527132 11.14146 220-229
TTCAG 719005 1.5507076 7.842908 150-159
TGACT 718805 1.5502762 10.666851 190-199
GTTGT 940040 1.5455294 5.3283224 220-229
GAATT 566765 1.5371772 12.19972 170-179
AGATC 592965 1.5366526 8.3039055 130-139
GGAAT 647030 1.5358635 10.544087 170-179
TGGTT 928340 1.5262933 20.122652 10-14
AGCCG 845290 1.5245321 10.895582 120-129
CCTCA 675000 1.5186085 9.425875 130-139
TAGTT 665795 1.5028403 10.031856 220-229
GCTCA 727745 1.4996947 10.35832 150-159
GTCAC 721285 1.4863822 6.4689837 140-149
GATCT 686565 1.4807429 6.6568017 130-139
CCATC 655270 1.4742202 14.433905 240-247
TGATA 541145 1.4676908 6.665494 180-189
ACTTC 622275 1.465206 7.9983225 220-229
CCACC 680295 1.462396 8.479366 230-239
ACGGA 642020 1.4561377 10.469046 200-209
GTAGG 841895 1.4556091 6.4226975 160-169
CCTTG 847015 1.4526672 12.190295 20-24
AACTG 560045 1.4513413 7.9783993 160-169
CCTCG 881790 1.4449933 8.835232 130-139
AGAAC 463250 1.4424828 8.966734 160-169
CTGGT 914310 1.4363163 5.4470625 160-169
GTGTT 863115 1.4190562 8.169347 160-169
GGCCG 1074550 1.4116144 10.7676 120-129
CTGCT 820550 1.4072785 5.874486 140-149
ATAAC 393140 1.3987342 9.071345 80-89
TTGGC 888425 1.3956528 10.96365 60-69
GAGAA 488525 1.3933609 8.568656 160-169
TCCCA 609235 1.370651 10.318156 240-247
GGACG 828905 1.3693587 5.269172 170-179
ACTGA 527310 1.3665094 6.8206596 180-189
TCACG 662825 1.3659112 10.258245 200-209
GGTCA 715030 1.3496758 5.11485 130-139
GTCCA 653420 1.3465301 20.859163 70-79
CATAC 467930 1.3238717 24.913359 70-79
TTGTA 582625 1.315108 7.0344043 220-229
AATAG 401145 1.3072871 8.486169 240-247
ATCAT 440115 1.3031833 6.9146476 240-247
CACTG 621065 1.2798548 5.3960767 230-239
CCCAC 588660 1.2654128 10.451906 240-247
ATATC 423605 1.2542971 8.701082 70-79
GTGGT 870975 1.2532678 14.220115 50-59
CAGCG 691255 1.2467206 8.100546 160-169
GGCTC 829870 1.2456393 8.034545 130-139
CTGAT 576500 1.2433612 6.722496 180-189
TGGTC 787480 1.2370752 15.59829 50-59
TATGG 626100 1.2368678 5.6656127 190-199
ATAGA 378265 1.2327236 8.919811 240-247
GGAGT 708115 1.2243077 7.9939766 210-219
TAACT 412285 1.2207786 6.5006 240-247
TATAC 409870 1.2136277 5.2202835 110-119
GCATA 466955 1.2101011 8.508687 220-229
TTCCA 508150 1.196488 9.413992 230-239
TCTGT 664390 1.1925385 7.065986 210-219
TCCAC 529770 1.1918714 8.702462 240-247
TGCCC 720025 1.1799083 7.1844997 60-69
AAATA 261600 1.1704389 5.4932737 9
AAAAG 298530 1.1689776 17.539701 70-79
AGGTT 590300 1.1661445 27.198355 10-14
TGGAC 617305 1.1652123 8.2430725 170-179
CATCA 410750 1.1620976 8.52102 230-239
AGCTT 536820 1.1577816 5.507137 150-159
TTGGT 699900 1.1507128 13.371125 25-29
AGCGT 608505 1.1486015 8.052724 160-169
GAAGA 402155 1.1470182 20.421364 35-39
CTCTT 583145 1.1427319 6.4751043 200-209
GAGGG 748880 1.1331992 5.798478 200-209
CGGCT 753715 1.1313303 7.1966643 130-139
GCTGA 594460 1.1220905 11.335659 30-34
CGAAC 451000 1.1167316 12.145974 70-79
CTCGG 743770 1.1164027 8.107196 130-139
AAGTA 342330 1.1156155 15.432906 70-79
CTACC 495450 1.1146585 8.732398 230-239
CATGT 516495 1.113946 7.1555095 160-169
CTTGA 516170 1.1132449 6.480018 200-209
TATCA 371020 1.0985926 8.465889 70-79
AACTT 370085 1.0958241 6.9868 210-219
ACTAT 367555 1.0883328 6.0693626 230-239
AGAGC 479350 1.0871928 6.0289884 140-149
GTGGA 626755 1.083639 12.233021 25-29
TATAA 290550 1.0818902 10.039288 240-247
TGCGT 687445 1.0799274 8.548887 210-219
AAAGT 330580 1.0773236 18.958124 70-79
ATAAA 240330 1.0752734 6.0000124 7
CTGCC 655765 1.0746051 6.5634913 200-209
CGTGG 779925 1.072301 9.825941 50-59
CCATG 519480 1.0705142 7.223599 150-159
CAATA 300260 1.0682809 6.788143 100-109
TAGGC 564510 1.0655574 7.3555627 160-169
ACGTC 516515 1.064404 14.396583 70-79
AGTGA 447210 1.0615481 5.143896 160-169
ACGTG 561240 1.059385 6.6979513 170-179
CCGTA 511070 1.0531834 10.14674 70-79
CTTCT 535550 1.0494646 6.2765117 210-219
AAGAG 367445 1.0480192 15.729785 35-39
CGGAG 629975 1.0407245 7.7351346 210-219
AGTTC 480880 1.0371336 5.288273 160-169
TTGAG 519205 1.0256954 6.320404 200-209
AGAGA 358375 1.0221497 14.784044 35-39
CGACT 492075 1.0140396 6.492713 200-209
TCCCC 565390 1.0115031 5.9991565 90-99
AAAAT 226015 1.011226 6.119344 90-99
CTGAA 387950 1.0053618 15.958113 35-39
TATGT 442595 0.99903065 6.9444246 220-229
ATCCA 352830 0.99822974 14.745768 240-247
TCGAC 483440 0.9962451 6.427173 190-199
CTGCG 661525 0.9929525 6.8512874 210-219
TGCTA 454930 0.98116606 5.143468 230-239
AGCTG 516410 0.97476476 5.9145074 90-99
ACAAT 273270 0.97225446 8.164129 100-109
ACTGG 513735 0.96971565 5.482646 160-169
GAGCT 512910 0.96815836 5.2884483 140-149
AGTTT 428675 0.96761024 5.7575207 220-229
TATTT 371555 0.95827055 7.015804 220-229
ACTCG 464645 0.95751345 6.1020856 190-199
TGTAA 352185 0.9551944 7.2759347 230-239
CTCGA 463015 0.95415443 5.815977 190-199
TCATA 321480 0.95190436 5.587935 240-247
ATGAT 349470 0.9478308 11.195879 240-247
TCTAC 402030 0.9466182 6.1562133 180-189
GACCG 524570 0.946094 22.099577 45-49
TACAA 263835 0.93868613 6.413657 100-109
CCATT 397475 0.93589306 6.7413826 50-59
TTCTG 519995 0.93335855 5.865561 210-219
GTCTA 431975 0.9316581 6.048463 180-189
TACTG 427600 0.9222224 5.394962 180-189
TAGAT 339995 0.9221328 6.8718266 240-247
CTTCC 491390 0.9200676 7.072462 230-239
GGGGT 725635 0.91382635 8.736712 10-14
GTATT 402575 0.908697 6.5409956 220-229
CCAGC 458635 0.9030598 6.3741016 80-89
ACTAA 253165 0.9007238 12.836503 240-247
ATCAA 252150 0.8971126 14.89797 70-79
GGAGG 590640 0.8937517 9.209835 30-34
CGTCC 541310 0.8870472 11.79693 70-79
TGCAT 410740 0.8858598 6.465921 210-219
ACCCA 323570 0.8746995 11.977566 240-247
GAGTT 440185 0.8695905 5.1094265 160-169
TATGA 319805 0.8673735 8.968704 240-247
TGAAG 362170 0.8596876 10.620524 35-39
TACCG 413490 0.8520962 13.437857 70-79
GGTCC 556035 0.8346115 15.321531 50-59
CCCCC 486350 0.8313687 5.107572 80-89
TTTAT 314875 0.8120883 6.1310925 220-229
AAAGA 205435 0.8044381 5.345572 8
ATTTA 257780 0.79884577 5.93613 220-229
TGAGA 334045 0.7929269 5.5952997 240-247
TCGAA 301955 0.78250813 15.485329 70-79
CCCAA 288275 0.7792874 7.706558 240-247
AGCCA 311030 0.7701486 6.9454913 80-89
ATGGG 438715 0.7585239 18.108501 15-19
AAAGG 263425 0.7513353 9.730939 10-14
CCGAA 302585 0.7492377 6.3035955 90-99
CATTG 345875 0.74596274 6.279925 50-59
GTAGC 395135 0.7458487 5.3867784 80-89
AAAGC 237575 0.7397687 11.703181 70-79
GCGTG 535850 0.73672783 5.5595098 160-169
AAGGT 307730 0.7304627 11.773127 10-14
TATTA 232345 0.7200241 5.4197016 230-239
CTAAT 238885 0.70734 9.7147255 240-247
TAACC 249955 0.7071749 5.8861113 80-89
CATAT 234670 0.6948594 6.5406847 240-247
AGCAG 299755 0.6798612 5.1971416 80-89
TGTTA 297875 0.672367 5.0671945 230-239
TAGGG 387400 0.66980195 5.0738635 10-14
ATCCC 294840 0.6633281 5.457702 240-247
CCAAT 232015 0.65641886 7.775041 240-247
CCCGA 333165 0.6560073 5.2034054 90-99
ATAGG 276040 0.65523964 5.519332 10-14
TAGGT 330225 0.6523632 6.279143 10-14
CCCAT 286520 0.64460987 5.4677305 240-247
GGTGG 484885 0.6106385 12.37749 25-29
AATCC 213980 0.6053941 7.2248187 240-247
AATGG 253115 0.6008224 7.096367 10-14
GAGGC 361940 0.5979282 7.339708 30-34
GAGGT 337615 0.58372533 8.137847 10-14
CATCC 258965 0.5826169 8.300581 240-247
AAGCA 184810 0.5754674 7.8176584 70-79
GTCGA 301400 0.56891644 10.2044115 70-79
GAAGG 269245 0.5593493 6.152166 10-14
CAATC 193885 0.5485411 7.310761 240-247
AAGGG 261880 0.5440488 7.1549163 10-14
GGGTC 392685 0.53989357 5.1511407 70-79
GATGA 222840 0.5289582 5.007084 240-247
ATAAG 159500 0.5197928 6.3218107 9
GGGCC 393185 0.5165191 9.234573 15-19
TGATC 239010 0.5154826 6.4557614 240-247
TGGGC 359165 0.49380767 10.051406 15-19
GAAAA 123485 0.48354 5.6616826 8
CGGTT 301500 0.4736351 9.296631 10-14
AGAGG 227480 0.4725836 5.333145 10-14
TAAGG 197910 0.4697815 6.536367 10-14
GATCC 217750 0.44872656 6.380362 240-247
TTGCC 257880 0.44227532 5.125384 60-69
AAATG 135200 0.44060183 5.3480167 9
AGAAG 152130 0.43390203 5.831828 9
GGTCG 303915 0.41784573 5.098031 70-79
TAAAA 92625 0.41441852 5.6549983 8
AAGAA 104905 0.41078487 5.8834324 9
CGATG 206120 0.3890679 7.7713466 15-19
TGGGT 261845 0.37677535 5.4793153 10-14
TAAAG 111175 0.36230698 6.1137013 9
AAGCT 124370 0.32230145 5.140144 30-34