FastQCFastQC Report
Thu 4 May 2017
HCGCLBCXY_s1_1_illumina12index_1_SL248356.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s1_1_illumina12index_1_SL248356.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 1895893
Filtered Sequences 0
Sequence length 50-251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 2244935 8.95257 59.513668 110-119
ATACA 2263315 8.626299 38.72986 110-119
CACAG 2931345 7.7756643 44.07751 110-119
CAGTA 2650715 7.3569546 42.91543 60-69
TCCCT 3609095 7.249583 117.01326 30-34
AGTAA 2033515 7.0934706 48.8079 110-119
GGAGA 2937510 6.527004 101.51271 35-39
GTAAT 2223080 6.455867 40.007282 110-119
GGTGA 3395285 6.280566 109.24917 45-49
CCCTG 3482205 6.1183815 79.56647 30-34
AATAC 1581470 6.0275455 41.974125 110-119
ACAGT 2047300 5.6822 36.148735 110-119
GTAGT 2667120 5.6402407 33.197853 80-89
AGACG 2308820 5.60522 105.98769 40-44
CTCAG 2377605 5.250472 38.376427 140-149
GTGAC 2565715 5.1856003 88.63285 45-49
ACTCC 2116305 5.1062837 132.72339 30-34
TAGTA 1714575 4.9791584 41.991318 80-89
GAGAC 2027590 4.9224663 148.30312 40-44
CCCCA 2078050 4.7920156 99.85111 60-69
CACTC 1927270 4.6501737 109.299446 25-29
GCACA 1733605 4.598548 35.049717 110-119
ATGCA 1651840 4.5846167 109.964615 25-29
ACCAG 1722730 4.569701 91.56766 45-49
AGTAG 1746875 4.4374022 31.985674 80-89
TGACC 1963605 4.336235 145.98865 45-49
CGTGT 2571245 4.3263545 21.215279 120-129
CTCCC 2253560 4.326328 75.93469 30-34
GGCCC 2787175 4.283651 59.828262 60-69
AGATA 1213060 4.231494 21.8138 150-159
GTGTC 2475230 4.1648006 21.998196 120-129
TACAC 1349315 4.091819 28.4305 110-119
CCCAG 1932580 4.078793 89.0217 60-69
GACCA 1505965 3.9947119 105.26118 45-49
AGGAG 1773760 3.9412081 102.6632 35-39
ATGGT 1861900 3.9374173 26.86182 190-199
TGCAC 1774450 3.9185233 130.7551 25-29
TGAGG 2108975 3.9011621 73.719536 35-39
GACGG 2175555 3.8461673 114.56537 40-44
CAGAT 1379315 3.8282342 20.406036 150-159
ACGGT 1885510 3.8108292 100.70282 40-44
GAGGA 1712650 3.8054247 133.30878 35-39
CTGAG 1880850 3.8014112 109.858475 35-39
CCTGA 1715115 3.787493 97.971466 30-34
GGATG 2030995 3.7569156 83.42911 20-24
TGTCC 1996100 3.6696868 19.799921 120-129
GCACT 1661010 3.6680129 101.47206 25-29
AGGCT 1756970 3.5510352 21.760395 140-149
CAGGG 2004925 3.5445104 36.98147 50-59
CGGAT 1743765 3.5243466 92.52882 20-24
GGGTT 2257210 3.4760227 42.477406 50-59
CCCTT 1716045 3.4470165 20.637667 50-59
CTCTC 1715145 3.4452093 13.53787 130-139
TCAGG 1685870 3.4073343 22.97971 140-149
GCCCC 2027500 3.4046986 72.65259 60-69
CCGTG 2110245 3.393494 21.676552 120-129
CCACT 1405975 3.3923779 26.5628 240-247
CTTCA 1338910 3.3802009 16.996449 200-209
GATGC 1671100 3.3774817 110.96688 25-29
TCTCA 1316745 3.3242433 22.53658 130-139
GGTTG 2099575 3.2332706 84.91373 15-19
CTTGG 1912840 3.2185283 15.041471 170-179
TGGTG 2087640 3.214891 18.721022 190-199
TCTTG 1661530 3.1960921 17.796556 170-179
TCGCA 1446795 3.194962 22.750408 110-119
CGCAC 1491415 3.1476953 23.09016 100-109
ACAGC 1186310 3.1467972 13.55297 120-129
CCAGG 1619230 3.1277692 40.08142 50-59
GTTGG 2015325 3.103529 61.281773 15-19
CCTGG 1911065 3.0731916 27.50184 50-59
AGGGT 1656600 3.0643632 52.608982 50-59
GCGGA 1703375 3.0114 104.64197 20-24
TCCAT 1189425 3.0028124 22.396948 70-79
TGTCT 1520360 2.92454 19.424538 180-189
TCTCT 1390405 2.922271 15.021233 180-189
TGGCC 1815090 2.9188545 44.256435 60-69
AGATG 1143015 2.9034808 19.1438 180-189
CGGTG 1958880 2.8830624 72.10996 40-44
AGTCA 1037450 2.8794014 31.1906 70-79
ACCAC 971280 2.8150303 16.996378 230-239
GCCGT 1738385 2.7955043 20.412334 120-129
CAGCT 1257515 2.7769737 13.382805 150-159
CCAGT 1255250 2.771972 38.647743 60-69
TTCCC 1376830 2.7656364 45.091354 50-59
TGTTC 1434030 2.7584772 14.744732 150-159
TCAAA 723485 2.757459 55.084564 70-79
GGCTG 1873090 2.7567976 14.091542 140-149
GATGG 1488865 2.7540884 15.12038 190-199
GAGAT 1079660 2.7425468 18.43193 180-189
CGGCC 1779040 2.734233 11.198949 120-129
GGTTC 1621760 2.7287593 46.601997 50-59
TTGGG 1763125 2.7151499 92.202995 15-19
ACTAC 891955 2.7048676 16.176685 240-247
TCCTC 1340935 2.6935344 17.977722 130-139
GCCCT 1529215 2.6868956 16.700851 200-209
GGCGG 2073895 2.669934 51.188316 20-24
TTCTT 1213365 2.6683028 20.214233 170-179
TACCA 856815 2.598305 18.209448 230-239
CACGG 1331930 2.572809 10.412149 120-129
CTGGA 1259400 2.5453901 14.615562 180-189
TCATT 952650 2.5164537 18.892279 150-159
TACAG 902765 2.5055885 17.068916 160-169
GATAC 900545 2.499427 16.54952 150-159
CATTT 942395 2.4893646 18.932823 150-159
TGGGG 1838830 2.4769645 63.152332 15-19
GCTGT 1462505 2.4607983 11.301836 140-149
TCAGC 1112340 2.4563837 15.008633 140-149
GTGAA 966970 2.4562922 18.990433 190-199
GTTCC 1335070 2.4544306 51.17349 50-59
ATTTG 1014830 2.4534707 17.121162 150-159
TGAAT 843595 2.449816 16.69753 190-199
ACACA 669265 2.4378846 16.358738 120-129
TTCAT 921780 2.4349098 17.381498 140-149
GACTC 1095055 2.4182134 16.268312 190-199
ACAGA 724500 2.4153795 10.18276 140-149
TTGTC 1247400 2.3994787 14.530573 170-179
CAGGC 1236600 2.388666 18.316362 140-149
CGTAG 1176420 2.377678 13.39119 80-89
AATCG 854820 2.3725193 16.502014 190-199
TGGAG 1280440 2.368546 14.394436 180-189
TTCAC 934460 2.35913 18.839102 200-209
GTTCT 1223425 2.3533607 16.933702 160-169
GAATC 844195 2.3430297 16.95408 190-199
TGGAA 916465 2.3279996 11.176716 170-179
TACTA 730175 2.3168314 13.932142 240-247
CTACT 898935 2.2694438 9.846888 230-239
TCACA 745905 2.2619689 8.606593 140-149
AGCTC 1024040 2.2613907 13.547941 140-149
GGGGC 1739935 2.2399933 58.28602 15-19
CTCTG 1212465 2.22903 14.879465 180-189
CTGGC 1378640 2.2169974 32.962917 60-69
GTCCT 1205325 2.2159035 15.888749 130-139
CAGCC 1046055 2.207744 11.875106 120-129
TAGTC 952565 2.2009883 28.137783 70-79
GTTCA 952490 2.200815 15.350278 140-149
ATCTC 862220 2.1767533 7.969895 100-109
GGGCG 1690135 2.1758811 71.11688 20-24
AGGGA 977055 2.1709685 6.9328384 160-169
TTTGC 1127445 2.168735 13.120318 150-159
ACTCT 858280 2.1668065 12.730586 200-209
TCTGC 1173825 2.157993 12.802666 210-219
TTGCA 931860 2.1531475 15.232011 150-159
AGTAT 741170 2.1523716 15.363581 220-229
AATAA 446640 2.1395059 9.100627 80-89
CTCGC 1211855 2.1292808 15.466644 100-109
CATAG 759865 2.1089752 9.057609 220-229
AACCA 575700 2.097062 14.172012 70-79
TTGGA 989660 2.0928645 15.615003 170-179
ACACG 786690 2.0867682 14.504941 120-129
TCGGC 1284780 2.0660608 8.33247 190-199
CACTA 677965 2.0559392 15.413369 240-247
CCATA 675460 2.0483427 27.100147 70-79
GATAT 703950 2.044284 7.078263 180-189
ATATG 702005 2.0386362 7.35754 180-189
GCAGA 827660 2.009345 15.954714 150-159
CCTTC 994330 1.997309 13.7770605 200-209
TCTCG 1082350 1.9898229 15.613978 100-109
AATAT 498940 1.9897213 10.958035 110-119
TGTAG 927900 1.9622587 6.641242 150-159
GAACT 706140 1.9598635 8.25882 160-169
GTCTC 1064880 1.9577055 12.154498 180-189
CTGTT 1009150 1.9411851 13.545583 140-149
CCAGA 729360 1.934695 31.157166 60-69
TGCAG 955780 1.9317396 14.267152 150-159
AAAAA 335520 1.9305731 11.425558 120-129
ACGGC 999260 1.9302105 13.588918 120-129
ATTGT 796335 1.9252331 8.894914 180-189
CAGAG 791975 1.9227111 7.133638 140-149
ACCGT 868565 1.9180543 24.120792 45-49
GAGTC 947245 1.9144894 13.170278 210-219
GTCAA 674920 1.8732138 36.89775 70-79
ATATA 466795 1.8615304 10.211026 240-247
CAAAA 399000 1.8266877 23.684713 70-79
ACCAT 597580 1.8121707 13.505783 45-49
AGTCT 780500 1.8034164 13.91804 210-219
CTCCA 747230 1.8029387 8.845292 150-159
ATAGT 613200 1.7807447 8.961268 220-229
CTGTG 1053520 1.772644 5.957442 210-219
ATGTA 609270 1.7693317 8.337098 160-169
GACGT 872395 1.7632091 10.878762 60-69
TCAGA 628345 1.7439468 10.841226 130-139
ATACC 569120 1.7258655 20.03993 70-79
TGCTC 931175 1.7118984 6.677215 150-159
TGTGC 1016460 1.710287 8.555076 220-229
GCTCT 924770 1.7001233 10.923402 130-139
TCTGG 1006650 1.6937807 11.823709 180-189
GTCTG 998060 1.6793272 9.675522 210-219
GTCCC 945045 1.6604842 23.657455 50-59
GTGCT 976415 1.6429075 7.3293424 220-229
CACCA 553435 1.6040032 10.982773 230-239
AATTG 551655 1.6020168 11.11347 170-179
CAGAC 595635 1.579977 19.785069 60-69
GAACC 594505 1.5769796 11.785007 70-79
GCTCC 888270 1.5607281 7.393639 150-159
TTCAG 673765 1.5567954 7.8726034 140-149
ATCGG 769595 1.5554386 9.557967 190-199
CAAAG 466075 1.5538275 26.700785 70-79
GCGTA 768565 1.5533569 11.169131 220-229
TGACT 671235 1.5509496 10.64139 190-199
GTTGT 877115 1.5441862 5.322238 210-219
AGATC 554595 1.5392565 8.403134 130-139
GAATT 527275 1.5312166 12.178203 170-179
GGAAT 600295 1.5248662 10.503694 170-179
TGGTT 863990 1.5210792 20.093246 10-14
AGCCG 785360 1.5170327 10.78561 120-129
CCTCA 625735 1.5097919 9.554979 130-139
GCTCA 680635 1.5030483 10.3400545 150-159
TAGTT 618000 1.4940873 10.018253 220-229
GTCAC 675865 1.4925147 6.43457 140-149
GATCT 643010 1.4857332 6.6846066 130-139
ACTTC 584775 1.476318 7.947723 220-229
CCATC 611815 1.4762052 14.454613 240-247
TGATA 506310 1.4703339 6.644804 180-189
CCACC 637025 1.4689895 8.400128 230-239
GTAGG 789730 1.4608352 6.494758 160-169
CCTTG 792555 1.4570554 12.232409 20-24
ACGGA 599145 1.4545696 10.470863 200-209
AACTG 522505 1.4501921 7.9318447 160-169
CCTCG 822660 1.4454484 8.843656 130-139
AGAAC 432835 1.4430101 8.872503 160-169
CTGGT 852890 1.4350656 5.3846254 170-179
CTGCT 772865 1.4208571 5.951904 140-149
GTGTT 802290 1.4124546 8.126043 160-169
GGCCG 1001205 1.4083313 10.82615 120-129
ATAAC 367510 1.4007114 9.085629 80-89
GAGAA 456950 1.3942726 8.518897 160-169
TTGGC 827340 1.3920752 10.984067 60-69
TCCCA 569320 1.3736722 10.496435 240-247
GGACG 774915 1.3699735 5.235939 170-179
ACTGA 493130 1.3686628 6.7555313 180-189
TCACG 619770 1.36864 10.25489 200-209
GTCCA 611340 1.350024 20.912409 70-79
GGTCA 666390 1.3468497 5.0641575 130-139
CATAC 437005 1.3252245 24.970028 70-79
TTGTA 544230 1.3157399 6.9733667 220-229
AATAG 373845 1.3040764 8.466073 240-247
ATCAT 410800 1.3034606 6.8964286 240-247
CACTG 581885 1.2849782 5.4126334 230-239
CCCAC 547745 1.2631085 10.362745 240-247
ATATC 395235 1.2540734 8.696992 70-79
GTGGT 813140 1.2522066 14.19562 50-59
CAGCG 644580 1.2450964 8.055692 160-169
CTGAT 538610 1.2445076 6.6390047 180-189
GGCTC 769890 1.2380636 7.9793415 130-139
TATGG 584775 1.2366416 5.6065245 190-199
TGGTC 732135 1.231884 15.611088 50-59
ATAGA 352950 1.2311888 8.900215 240-247
GGAGT 663255 1.2268829 7.9641967 210-219
TATAC 385085 1.2218676 5.360905 110-119
TAACT 384420 1.2197576 6.453013 240-247
GCATA 435995 1.2100868 8.523593 220-229
TTCCA 477010 1.2042555 9.331758 230-239
TCTGT 621205 1.19494 7.1031203 210-219
TCCAC 495005 1.1943629 8.74703 240-247
TGCCC 671190 1.1793094 7.1722784 60-69
CATCA 385185 1.1680795 8.582239 230-239
AGGTT 550810 1.1648148 27.19011 10-14
AAATA 242840 1.1632582 5.5015006 9
TGGAC 575295 1.1627363 8.208434 170-179
AAAAG 276170 1.1571773 17.342957 70-79
AGCTT 498740 1.1523842 5.4452543 150-159
AGCGT 568740 1.149488 7.991506 160-169
CTCTT 545575 1.1466572 6.467016 200-209
TTGGT 650945 1.1460074 13.460773 25-29
GAAGA 374570 1.1429098 20.244831 35-39
GAGGG 702365 1.136457 5.7173142 200-209
CGGCT 701410 1.1279404 7.199774 130-139
GCTGA 555545 1.1228193 11.360313 30-34
AAGTA 321850 1.1227033 15.499511 70-79
CTTGA 484345 1.1191232 6.4389343 200-209
CTCGG 695210 1.1179703 8.09085 130-139
CATGT 482310 1.1144212 7.155942 150-159
CTACC 460155 1.1102757 8.699449 230-239
CGAAC 416545 1.1049242 12.061948 70-79
AACTT 347845 1.1037058 7.0354824 210-219
TATCA 346400 1.0991207 8.474612 70-79
AAAGT 311840 1.0877855 19.044868 70-79
ACTAT 342660 1.0872537 5.988319 230-239
TATAA 272455 1.0865225 10.055638 240-247
AGAGC 446915 1.0849944 5.9541426 140-149
GTGGA 585500 1.0830524 12.233478 25-29
TGCGT 642415 1.0809219 8.505939 210-219
CTGCC 614030 1.0788769 6.563878 200-209
CGTGG 729020 1.0729651 9.850821 50-59
CCATG 485455 1.0720315 7.2694187 150-159
ATAAA 223635 1.0712619 5.7037616 7
ACGTC 483885 1.0685647 14.463822 70-79
TAGGC 528540 1.0682392 7.441778 160-169
CAATA 279460 1.0651214 6.809883 100-109
ACGTG 523760 1.0585783 6.701955 170-179
CCGTA 479100 1.0579978 10.0918255 70-79
CTTCT 502750 1.0566502 6.363467 210-219
AGTGA 415000 1.0541807 5.105073 160-169
AAGAG 342275 1.0443695 15.564059 35-39
CGGAG 588475 1.0403659 7.6772246 210-219
AGTTC 449515 1.0386453 5.270377 160-169
TTGAG 485745 1.0272199 6.278468 200-209
AGAGA 335035 1.0222782 14.608465 35-39
CGACT 461745 1.0196728 6.4371014 200-209
AAAAT 210330 1.007528 6.083529 90-99
TCCCC 524435 1.0067971 5.9454474 90-99
CTGAA 361485 1.0032874 15.926488 35-39
TCGAC 453380 1.0012006 6.4240384 190-199
TATGT 412785 0.9979561 6.974945 220-229
CTGCG 617310 0.992699 6.830923 210-219
ATCCA 327015 0.9916781 14.736807 240-247
TGCTA 425290 0.98267126 5.125658 230-239
AGCTG 482475 0.9751366 5.9625077 90-99
ACAAT 254785 0.9710762 8.114394 100-109
ACTGG 479415 0.968952 5.42753 160-169
ACTCG 437135 0.96532655 6.1397367 190-199
GAGCT 477445 0.9649704 5.2147074 140-149
TATTT 347730 0.9610859 6.9692154 220-229
CTCGA 434725 0.9600046 5.8089795 190-199
AGTTT 397065 0.95995104 5.737979 220-229
TCATA 301925 0.95800245 5.464455 240-247
TGTAA 327585 0.9513132 7.19842 230-239
GACCG 492240 0.9508304 22.054327 45-49
ATGAT 326585 0.9484091 11.022332 240-247
TCTAC 375000 0.94672185 6.0567107 180-189
TTCTG 489075 0.94077677 5.9233413 210-219
TACAA 245885 0.9371551 6.355336 100-109
GTCTA 404030 0.9335481 6.0042105 180-189
TAGAT 321180 0.93271285 6.834648 240-247
CCATT 368190 0.9295293 6.699514 50-59
TACTG 400125 0.92452526 5.3552394 180-189
CTTCC 460140 0.92428255 7.019464 230-239
GGGGT 679620 0.9154704 8.722613 10-14
GTATT 376065 0.9091812 6.5230436 220-229
CCAGC 430675 0.908958 6.459286 80-89
ATCAA 234255 0.89282906 14.816483 70-79
GGAGG 551540 0.8924156 9.2153635 30-34
ACTAA 233735 0.89084715 12.778677 240-247
TGCAT 384575 0.8885956 6.4811034 210-219
CGTCC 505235 0.88771933 11.837482 70-79
ACCCA 300480 0.8708717 11.864877 240-247
GAGTT 411165 0.86950326 5.057799 160-169
TATGA 296640 0.8614482 8.811276 240-247
TACCG 387475 0.8556622 13.464486 70-79
TGAAG 335585 0.85245126 10.556485 35-39
GGTCC 518380 0.8336092 15.316836 50-59
CCCCC 452990 0.8311405 5.144697 80-89
TTTAT 295415 0.8164932 6.150412 220-229
ATTTA 242480 0.80502224 5.9537063 220-229
TGAGA 314595 0.7991326 5.513977 240-247
AAAGA 189780 0.7951954 5.065 8
TCGAA 280700 0.7790718 15.3929 70-79
GTACC 351535 0.7762958 5.050031 80-89
CCCAA 266540 0.7725045 7.6973987 240-247
AGCCA 290695 0.77109545 7.038081 80-89
ATGGG 411825 0.76179004 18.169905 15-19
GTAGC 369630 0.74706405 5.4219832 80-89
AAAGG 243430 0.7427678 9.721555 10-14
CATTG 320085 0.7395856 6.23841 50-59
CCGAA 277950 0.7372882 6.161453 90-99
GCGTG 500070 0.73599863 5.520385 160-169
AAGGT 287975 0.7315126 11.792693 10-14
AAAGC 218060 0.726981 11.486588 70-79
TATTA 217790 0.7230526 5.3922963 230-239
TAACC 235025 0.71271706 5.9105406 80-89
CATAT 220260 0.6988808 6.486921 240-247
CTAAT 220080 0.6983098 9.60805 240-247
AGCAG 279605 0.67880887 5.141388 80-89
TAGGG 366205 0.67740256 5.1611795 10-14
TGTTA 277225 0.67022395 5.0808268 230-239
ATCCC 274830 0.663118 5.362861 240-247
ATAGG 260030 0.6605269 5.603077 10-14
TAGGT 309090 0.6536421 6.3136935 10-14
CCCGA 309160 0.65249544 5.10907 90-99
CCAAT 214990 0.65196055 7.7193985 240-247
CCCAT 267810 0.64617985 5.5413713 240-247
GGTGG 454155 0.61176175 12.377674 25-29
AATCC 199370 0.60459274 7.2457557 240-247
AATGG 237210 0.6025596 7.1794405 10-14
GAGGC 339370 0.5999729 7.384545 30-34
GAGGT 317380 0.58708656 8.106854 10-14
CATCC 239220 0.57719713 8.26267 240-247
GTCGA 280805 0.5675387 10.176648 70-79
GAAGG 255355 0.5673863 6.161497 10-14
AAGCA 168960 0.5632885 7.6296587 70-79
CAATC 180765 0.5481727 7.2971077 240-247
AAGGG 244050 0.54226726 7.141505 10-14
GGGTC 367775 0.541288 5.1538367 70-79
GATGA 211945 0.5383816 5.022425 240-247
ATAAG 148315 0.51736444 6.0766883 9
TGATC 223855 0.51723737 6.397588 240-247
GGGCC 367280 0.5166293 9.24263 15-19
TGGGC 336140 0.49472788 10.043969 15-19
GAAAA 116360 0.4875589 5.6513667 8
AGAGG 214220 0.4759864 5.2458234 10-14
CGGTT 278445 0.46850914 9.137636 10-14
TAAGG 183955 0.46728155 6.4739995 10-14
GATCC 202715 0.44765607 6.3096995 240-247
TTGCC 239985 0.44119522 5.1657104 60-69
AGAAG 143415 0.43759617 5.8270426 9
AAATG 125320 0.4371514 5.3192067 9
GGTCG 283765 0.41764286 5.100305 70-79
TAAAA 86740 0.4155041 5.894465 8
AAGAA 97290 0.40765393 5.348074 9
CGATG 195540 0.39520848 7.9691896 15-19
TGGGT 249080 0.3835743 5.4994597 10-14
TAAAG 103785 0.36203125 6.122979 9
AAGCT 114645 0.31819266 5.060609 30-34