FastQCFastQC Report
Thu 4 May 2017
HCGCLBCXY_s2_1_BC_X.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s2_1_BC_X.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9231713
Filtered Sequences 0
Sequence length 50-251
%GC 52

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TCCCT 14577280 6.780247 104.93836 30-34
TAATA 9123145 6.326506 41.43561 110-119
CAGTA 11183085 6.1148114 35.928837 60-69
CACAG 11350050 6.1132746 35.23908 110-119
CCCTG 14585520 6.1109657 73.2478 30-34
ATACA 8673545 5.9247594 28.19353 110-119
GGTGA 13397875 5.3625207 91.810936 45-49
GGAGA 11681610 5.261471 79.69667 35-39
AGTAA 8304225 5.1872516 34.856564 110-119
GTAAT 8813105 4.892104 30.425577 110-119
GTGAC 10401980 4.5528655 73.95092 45-49
AGACG 9165090 4.5141606 83.26347 40-44
ACTCC 8578775 4.490201 113.361786 30-34
CTCAG 9358675 4.4793916 32.1766 140-149
AATAC 6504655 4.4432254 30.596552 110-119
GTAGT 9969655 4.429904 20.90362 80-89
ACCAG 8223605 4.4293337 78.40076 45-49
ACAGT 8020925 4.3857703 28.24601 110-119
CCCCA 8266590 4.26208 86.24958 60-69
CACTC 7997490 4.1859517 92.364426 25-29
CTCCC 9050390 4.146589 70.51656 30-34
GGCCC 10847440 4.093869 58.392193 60-69
GAGAC 8224655 4.0509605 116.825165 40-44
TGACC 8320320 3.9823985 121.58344 45-49
GACCA 7162615 3.8578713 88.13445 45-49
ATGCA 7048090 3.8538332 84.54902 25-29
CGTGT 9850965 3.8315654 19.474504 120-129
GTGTC 9691430 3.7695134 20.338503 120-129
GCACA 6884110 3.7078655 27.866043 110-119
CCCAG 7749040 3.6534848 76.642586 60-69
GACGG 9171535 3.6160111 98.46355 40-44
AGTAG 7123505 3.5618806 23.658281 60-69
CCTGA 7423720 3.5532544 82.15574 30-34
TGAGG 8860930 3.5466013 62.445866 35-39
TGCAC 7364385 3.5248542 110.428795 25-29
CAGGG 8825570 3.4796095 35.16242 50-59
GGGTT 9745055 3.4661381 41.875076 50-59
ACGGT 7907930 3.4612398 83.7797 40-44
AGGAG 7623340 3.4336004 80.680046 35-39
CTGAG 7810865 3.4187548 93.5035 35-39
GGATG 8497400 3.4010978 69.94089 20-24
ATGGT 7605440 3.3793917 22.168127 190-199
GCCCC 8062750 3.327559 69.044876 60-69
GCACT 6929750 3.316823 85.46553 25-29
TGTCC 7682095 3.2674794 18.295982 120-129
TAGTA 5865140 3.2557058 23.360077 80-89
GAGGA 7143595 3.2175202 104.9203 35-39
GGTTG 8897705 3.1647515 77.168106 15-19
GATGC 7208480 3.1550956 94.19953 25-29
CGGAT 7207550 3.154689 77.693596 20-24
TACAC 5244715 3.1360226 22.749556 110-119
CCTGG 8077485 3.0947692 27.633184 50-59
CCAGG 7116830 3.068387 38.14285 50-59
AGGGT 7631390 3.054476 48.320625 50-59
AGGCT 6955485 3.044362 18.28343 140-149
CTCTC 6542415 3.0430362 12.441403 100-109
GTTGG 8537575 3.0366595 55.36527 15-19
CAGAT 5530135 3.0238285 15.332995 150-159
TCAGG 6858100 3.001737 19.34402 140-149
CCACT 5730860 2.999579 22.299444 240-247
CCGTG 7732580 2.9626238 20.380936 120-129
TGGTG 8320155 2.9593275 16.970829 190-199
TGGCC 7703330 2.951417 42.385338 60-69
TCTTG 6718365 2.9009624 15.456424 170-179
AGATA 4643070 2.9003038 14.936063 150-159
CTTGG 7410890 2.8824894 13.516169 170-179
TTCCC 6125795 2.849256 44.46532 50-59
GCGGA 7158395 2.8223014 90.77795 20-24
GGTTC 7208390 2.803727 46.055923 50-59
GGCGG 8861055 2.796537 49.046375 20-24
CGGTG 7974145 2.793833 65.89158 40-44
TCGCA 5818710 2.7850394 19.571226 100-109
TCTCA 5213005 2.7699628 18.51813 130-139
CCCTT 5947955 2.7665389 15.922622 20-24
CTTCA 5178240 2.75149 13.440173 200-209
CGCAC 5743570 2.7079537 20.652536 100-109
TCAAA 3963580 2.7074575 41.78084 70-79
CCAGT 5639745 2.6993809 34.970417 60-69
GCCGT 6980625 2.6745234 19.337076 120-129
TTGGG 7451160 2.6502419 82.93373 15-19
AGTCA 4844015 2.6486642 27.217403 70-79
TGTCT 5977085 2.5808806 16.721296 180-189
GTTCC 6048015 2.5724447 50.70021 50-59
GGCTG 7340200 2.5717227 12.932476 140-149
TCTCT 5412315 2.5556264 12.668587 180-189
CGGCC 6698445 2.5280216 11.038359 120-129
CAGCT 5261570 2.518373 11.837964 150-159
ACAGC 4605650 2.480659 10.778914 120-129
CTGGC 6459760 2.4749615 33.887688 60-69
TCCAT 4656855 2.474449 13.775484 70-79
TGGGG 7655445 2.4527364 58.405685 15-19
TTCTT 5068180 2.429471 16.876688 170-179
TGTTC 5618870 2.426205 12.095346 150-159
TCCTC 5153255 2.396904 15.840326 130-139
TCAGC 4994905 2.3907375 13.330798 140-149
GATGG 5923495 2.3708882 12.235883 190-199
GCCCT 5641960 2.3638391 15.122852 200-209
ACCAC 3967695 2.3369544 13.423018 230-239
AACCA 3445260 2.3181992 12.026721 70-79
AGATG 4612950 2.3065581 14.565713 180-189
TACCA 3852210 2.3033888 14.196618 230-239
GGGGC 7190290 2.2692459 55.53128 15-19
AAAAA 2552365 2.241326 5.7743607 8
GCTGT 5729465 2.2284944 9.881664 140-149
GGGCG 7043860 2.2230327 68.53015 20-24
GAGAT 4361970 2.1810637 14.060677 180-189
CACGG 5037405 2.1718528 9.386794 120-129
CATTT 4021430 2.1692603 14.625049 150-159
GTCAA 3952805 2.1613586 32.5145 70-79
GACTC 4512710 2.1599422 14.810082 190-199
TCATT 3973070 2.1431735 14.552392 150-159
CAGGC 4922930 2.1224976 15.681012 140-149
GTTCT 4876520 2.1056612 15.059392 160-169
CTGGA 4806055 2.103573 11.888113 180-189
TTCAT 3883720 2.0949762 13.947571 140-149
TGAAT 3760180 2.0872543 12.35331 190-199
TAGTC 4284130 2.0816736 25.850567 70-79
ATTTG 4193665 2.0686598 13.222361 150-159
CTCGC 4928680 2.0649924 14.899907 100-109
AGCTC 4264015 2.0409079 11.902126 140-149
ACTAC 3404310 2.0355716 11.491048 240-247
TCTGC 4769725 2.0287406 11.3354025 210-219
CAAAA 2626805 2.019172 15.372942 70-79
CAGCC 4277550 2.016761 10.489162 120-129
TTGTC 4665770 2.0146604 12.557646 170-179
GTCCT 4723205 2.008954 13.886293 130-139
TGGAG 4996945 2.000035 11.757056 180-189
ATCTC 3750920 1.9930748 6.4880624 100-109
CTACT 3743875 1.9893314 8.006047 230-239
ACAGA 3202610 1.9705931 8.167432 140-149
TGGAA 3903250 1.9516953 8.679829 170-179
GATAC 3542545 1.9370321 12.525718 150-159
GTTCA 3960490 1.9244157 12.681695 140-149
TCGGC 5003255 1.9169233 7.597056 190-199
ACCAT 3203610 1.9155651 9.755042 45-49
TACAG 3483980 1.9050094 12.466881 160-169
CTCTG 4461460 1.8976243 12.937269 180-189
AATAA 2411505 1.8818239 6.5755796 80-89
TTGGA 4222630 1.8762782 13.292842 170-179
TCTCG 4398965 1.8710427 14.0523405 100-109
TTTGC 4325170 1.8675908 11.263135 150-159
GAATC 3407735 1.8633193 12.669615 190-199
TTGCA 3829205 1.860624 12.269516 150-159
ACGGC 4291785 1.8503826 12.275367 120-129
TTCAC 3452715 1.8346217 14.890436 200-209
CCTTC 3934290 1.8299341 11.975937 200-209
ACTCT 3421105 1.8178257 10.900897 200-209
ACACA 2675115 1.7999945 11.887413 120-129
CTGTT 4160635 1.7965451 11.569964 140-149
GTGAA 3581840 1.7909845 14.0297 190-199
AGGGA 3972485 1.7892324 5.661633 160-169
AATCG 3263670 1.7845458 12.221638 190-199
TCACA 2983535 1.7839735 7.182156 140-149
CCATA 2955535 1.7672313 15.805186 70-79
AATAT 2545560 1.7652357 7.630431 110-119
CGTAG 4030230 1.7640005 9.110739 80-89
GCAGA 3578895 1.7627442 12.506841 150-159
TACTA 2891065 1.7549326 9.577814 240-247
TGTAG 3929235 1.7459111 5.8269215 210-219
CATAG 3146975 1.720738 7.0279317 210-219
ACACG 3155700 1.6996983 11.788055 120-129
TGCAG 3856155 1.687809 11.996042 150-159
GTCTC 3959260 1.68402 10.36752 180-189
TGCTC 3956165 1.6827036 6.273787 150-159
GATAT 3028710 1.6812196 5.998547 180-189
CACTA 2807360 1.6786314 11.607466 240-247
TGGTT 4230020 1.6702641 17.692032 10-14
CTCCA 3130815 1.6386942 7.86794 150-159
AGTAT 2944325 1.634378 10.894142 220-229
ATTGT 3285235 1.6205475 7.0205936 170-179
CTGCT 3804710 1.6182841 5.9225454 140-149
CACCA 2740895 1.6143748 9.250885 230-239
GAGTC 3679935 1.6106789 10.594257 210-219
ATATG 2890775 1.6046528 6.061852 180-189
CTGTG 4114825 1.6004746 5.5668244 210-219
TCAGA 2924680 1.5991888 8.73806 130-139
GTGCT 4104510 1.5964626 7.047171 220-229
GCTCT 3741905 1.5915709 9.630123 130-139
CAAAG 2584710 1.5903939 22.275885 70-79
CCAGA 2942670 1.584958 19.72558 60-69
CTGGT 4071580 1.5836543 5.5385475 160-169
GAACT 2894100 1.5824682 6.9823723 160-169
TCTGG 4011620 1.5603324 10.331265 180-189
TTCAG 3205810 1.5577141 7.1175203 150-159
TGTGC 3989930 1.5518962 7.7276344 220-229
CAGAG 3133980 1.5436062 6.1993265 140-149
GCTCC 3593900 1.5057536 7.1767535 150-159
GCTCA 3138665 1.5022758 9.449416 140-149
GTCTG 3811195 1.4823767 8.445829 210-219
CCATC 2828605 1.480515 12.324217 240-247
CCTCA 2801575 1.4663672 8.310265 130-139
AGTCT 2982810 1.4493576 10.952828 210-219
ATAGT 2609720 1.4486408 6.629516 220-229
ATAAC 2115745 1.4452314 7.296934 80-89
GACGT 3296755 1.4429642 6.7679024 60-69
ACCGT 2968535 1.4208455 14.99963 45-49
TGACT 2922225 1.4199193 9.477007 190-199
ACTTC 2649055 1.4075919 6.6921225 210-219
GGCCG 4062925 1.4021993 10.589256 120-129
ATACC 2341965 1.4003536 11.47779 70-79
GTCCC 3331995 1.3960218 17.161083 50-59
GAACC 2589940 1.3949732 10.413202 70-79
CCTTG 3277470 1.3940296 10.571246 20-24
ATGTA 2504640 1.3903115 6.4687886 160-169
AGCCG 3219605 1.3881172 9.508559 120-129
GTCAC 2890285 1.3833923 5.8094387 140-149
TGATA 2486810 1.3804141 5.723324 180-189
CCACC 2668285 1.3757116 7.3948545 230-239
GTTGT 3454830 1.3641728 5.0172033 210-219
GAGAA 2420865 1.3621585 6.870097 160-169
ATATA 1947720 1.3506595 7.1058836 240-247
GCGTA 3082065 1.348996 9.348298 210-219
AACTG 2462525 1.3464868 6.784038 160-169
CTGAT 2769665 1.3457898 6.149035 180-189
TTGGT 3406450 1.3450695 11.539163 25-29
CCTCG 3209935 1.3448819 7.938241 130-139
ACTGA 2434420 1.3311192 5.9706845 180-189
AGAAC 2137385 1.3151511 7.091375 160-169
ATCAT 2159690 1.3109738 5.539648 230-239
CAATA 1906415 1.3022414 5.4073553 100-109
CATCA 2176230 1.301254 6.9969783 230-239
AGCTT 2675695 1.3001294 5.1290894 150-159
ATCGG 2953280 1.2926277 7.8714976 190-199
GTAGG 3219765 1.2887161 5.705071 160-169
AATTG 2308595 1.281488 8.31846 170-179
TAGTT 2581095 1.2732079 8.489025 220-229
TATGG 2863970 1.2725729 5.031093 190-199
GTGTT 3222535 1.2724489 7.0816336 160-169
CTCTT 2680485 1.2656912 6.3281403 200-209
TTGGC 3249140 1.2637637 8.138374 60-69
AAAAG 1793935 1.2610037 10.870763 70-79
CAGCG 2906005 1.25291 7.027056 160-169
AATAG 1999410 1.2489358 6.3537364 240-247
TCCCA 2343625 1.2266724 8.843574 240-247
TGGTC 3143575 1.2227038 10.468209 50-59
CTTCT 2579610 1.2180592 6.23306 210-219
TTCCA 2288370 1.2159398 7.8453393 230-239
TATCA 1989740 1.2078109 5.987599 70-79
GAATT 2175090 1.2073802 8.978133 170-179
TTCTG 2785835 1.2029122 5.9475846 210-219
TCACG 2507380 1.2001203 8.6203575 200-209
ACGGA 2425410 1.1946082 8.172648 200-209
AAAGT 1909045 1.192489 15.629452 70-79
AGATC 2169385 1.1862005 6.643293 130-139
GGCTC 3090310 1.1840066 7.1609025 130-139
GCTGA 2695230 1.1796814 9.171542 30-34
CTTGA 2417735 1.1747857 6.0241823 200-209
TCTGT 2702490 1.166924 6.594959 210-219
ATATC 1920190 1.1655927 6.1610203 70-79
GATCT 2380805 1.1568414 5.5827885 130-139
TCCAC 2203260 1.1532043 7.819207 240-247
CCAGC 2435445 1.1482533 6.2520914 80-89
GGAAT 2293165 1.1466238 8.053617 170-179
AGGTT 2576960 1.1450433 21.81679 10-14
CATAC 1904100 1.1385367 14.248813 70-79
AAGAG 2013875 1.1331556 9.851589 35-39
CCATG 2367305 1.1330756 6.5647345 150-159
AGCGT 2586455 1.1320714 6.793498 160-169
AGAGC 2297210 1.1314647 5.4357386 140-149
TTGTA 2288900 1.1290733 5.721606 220-229
CAGAC 2081815 1.1212908 11.560273 60-69
GAGGG 3106255 1.1199253 5.596999 200-209
TGGAC 2548255 1.1153514 7.493137 170-179
GTGGT 3118830 1.1093109 9.432306 50-59
GCATA 2024405 1.1069267 6.6426167 220-229
GGAGT 2737270 1.0955967 6.520016 210-219
CGAAC 2020585 1.0883117 10.751938 70-79
GTCCA 2270525 1.0867532 13.289854 70-79
GAAGA 1923220 1.0821465 12.72478 35-39
CTGCC 2580210 1.0810429 6.392682 200-209
CGGCT 2809900 1.0765717 6.3877854 130-139
AACTT 1766930 1.0725608 5.6591578 210-219
TGCGT 2743560 1.0671166 7.6948028 210-219
CCCAC 2068570 1.0665112 8.550569 240-247
ATCCA 1778205 1.0632591 12.054817 240-247
TTGAG 2392075 1.0628916 5.7353315 200-209
AAGTA 1697560 1.0603845 11.940608 70-79
TGCCC 2529095 1.0596269 5.3649483 200-209
TACTG 2178870 1.0587205 5.0573993 180-189
CTCGG 2739800 1.0497139 7.3482647 130-139
ATAGA 1655555 1.0341461 6.74068 240-247
ACGTG 2353285 1.0300145 6.192449 170-179
AGCTG 2337630 1.0231625 5.300229 90-99
ATCAA 1489430 1.0174057 9.704636 70-79
CTACC 1942015 1.0164665 7.098048 230-239
CTGAA 1853270 1.0133516 11.107085 35-39
TAACC 1694485 1.0131996 5.5776534 80-89
ACTGG 2280420 0.9981221 5.156868 160-169
GTGGA 2482340 0.9935605 9.669822 25-29
TATTT 1807965 0.99007136 5.570983 220-229
CGACT 2066360 0.9890328 5.834381 200-209
TAGGC 2249085 0.984407 6.6339645 160-169
AAAGC 1596470 0.9823215 8.074483 70-79
CTGCG 2553550 0.97835493 6.2390795 210-219
TATAA 1410155 0.97788155 6.8683367 240-247
GTCTA 2006945 0.9751815 5.433411 180-189
TTTAT 1763925 0.96595436 5.0082026 220-229
CTTCC 2074820 0.9650491 6.3996186 230-239
ACAAT 1411250 0.96400213 6.1391106 100-109
GGAGG 2659325 0.95878965 7.61915 30-34
AAGCA 1551840 0.95486027 5.4522443 70-79
TCCCC 2081570 0.9537064 5.1748166 90-99
GGGGT 2952375 0.9459147 8.363861 10-14
CATGT 1937475 0.9414258 6.1032767 150-159
AGAGA 1658990 0.9334711 9.400154 35-39
CGGAG 2349805 0.9264448 6.393826 210-219
TATGT 1862040 0.918511 5.429475 220-229
CGTGG 2621090 0.9183289 6.7434893 50-59
ATGAT 1650920 0.91641635 8.567691 240-247
TCTAC 1709205 0.90819675 5.44711 180-189
AAAGG 1590105 0.89471114 7.2002673 10-14
ACTCG 1864660 0.892492 5.792151 190-199
CTCGA 1859930 0.89022803 5.408437 190-199
AGCCA 1646935 0.88705915 6.1062202 80-89
TCGAC 1836245 0.8788916 5.9273777 190-199
GTATT 1751955 0.8642079 5.2019362 220-229
AAGGT 1671880 0.8359701 8.9336405 10-14
TGCAT 1698255 0.825188 5.444948 210-219
TGAAG 1632895 0.8164768 7.33628 35-39
CGTCC 1919560 0.8042473 8.285519 70-79
ACGTC 1678615 0.8034443 9.119446 70-79
CCGTA 1666995 0.7978825 6.872498 80-89
CCAAT 1333060 0.7970894 6.5715494 240-247
ACTAA 1162645 0.79418415 8.678591 240-247
TGTAA 1417410 0.78679615 5.616208 230-239
CAATC 1315115 0.78635925 6.310491 240-247
TCGAA 1421090 0.77703935 12.694532 70-79
TAGAT 1385640 0.7691609 5.473566 240-247
TATGA 1379190 0.76558053 6.7007957 240-247
GCGTG 2158985 0.7564251 5.135848 160-169
ATGGG 1885810 0.7547984 14.873157 15-19
GACCG 1746345 0.7529282 14.293179 45-49
CCCAA 1252905 0.73795545 6.733892 240-247
TACCG 1535695 0.73503774 8.340819 70-79
ACCCA 1240940 0.73090804 9.229316 240-247
CTAAT 1200260 0.7285811 7.113009 240-247
GAGGC 1823135 0.7187975 5.9994755 30-34
AATCC 1199440 0.71719265 6.1129527 240-247
GGTGG 2162515 0.6928506 10.878415 25-29
AATGG 1379265 0.68965733 5.7146816 10-14
CATCC 1293565 0.67706245 7.40009 240-247
GGTCC 1731140 0.6632607 10.5982 50-59
GGGTC 1835040 0.64292717 5.3549933 70-79
CGGTT 1591625 0.6190678 8.433259 10-14
TAGGT 1384785 0.61531365 5.1487184 10-14
GAGGT 1524030 0.6099954 6.786393 10-14
GGTCG 1659980 0.5815929 5.408497 70-79
GTCGA 1316065 0.5760315 9.611995 70-79
GGGCC 1509820 0.52107006 8.544341 15-19
AAGGG 1129405 0.50869113 5.6921716 10-14
TGGGC 1444280 0.50601995 9.049941 15-19
TAAGG 999575 0.4998055 5.022527 10-14
TGGGT 1208455 0.4298254 5.2517395 10-14
CGATG 979250 0.42861018 6.3165584 15-19
TGATC 856080 0.41597223 5.3831387 240-247
GATCC 777705 0.37223703 5.4928327 240-247