FastQCFastQC Report
Thu 4 May 2017
HCGCLBCXY_s1_1_BC_X.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGCLBCXY_s1_1_BC_X.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9604831
Filtered Sequences 0
Sequence length 50-251
%GC 53

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[WARN] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TCCCT 15338645 6.764521 105.6715 30-34
TAATA 9643420 6.429742 42.241547 110-119
CACAG 12029140 6.1981287 35.749836 110-119
CAGTA 11788220 6.1636972 36.529106 60-69
CCCTG 15377975 6.1040387 73.66346 30-34
ATACA 9188250 6.0370975 28.85161 110-119
GGTGA 14232965 5.4090533 92.8128 45-49
GGAGA 12366470 5.3154736 80.81177 35-39
AGTAA 8764740 5.259804 35.490788 110-119
GTAAT 9335390 4.953276 30.848787 110-119
GTGAC 11011600 4.581853 74.85078 45-49
AGACG 9630660 4.5322847 84.60668 40-44
CTCAG 9931680 4.524581 32.56843 140-149
AATAC 6867395 4.512191 31.221523 110-119
ACTCC 9034095 4.5061445 114.743645 30-34
GTAGT 10606600 4.478514 21.176014 80-89
ACAGT 8486820 4.437496 28.633394 110-119
ACCAG 8611370 4.43709 79.578064 45-49
CCCCA 8697285 4.2750096 87.30522 60-69
CACTC 8412545 4.19612 93.335785 25-29
CTCCC 9553415 4.15185 71.10054 30-34
GGCCC 11488145 4.104272 58.73727 60-69
GAGAC 8671930 4.0810966 118.636795 40-44
TGACC 8745415 3.984153 122.9478 45-49
ATGCA 7397325 3.8678334 86.03101 25-29
GACCA 7497235 3.8630214 89.487915 45-49
CGTGT 10477200 3.8544776 19.613491 120-129
GTGTC 10313685 3.7943223 20.520962 120-129
GCACA 7272360 3.7471526 28.22244 110-119
CCCAG 8149235 3.6585195 77.54918 60-69
GACGG 9661800 3.6184022 99.4441 40-44
AGTAG 7503575 3.5834093 24.017889 60-69
TGAGG 9344540 3.5512705 63.011864 35-39
CCTGA 7789895 3.5488467 82.986664 30-34
TGCAC 7744035 3.5279536 111.61395 25-29
CAGGG 9299140 3.4825835 35.51308 50-59
GGGTT 10290815 3.4578426 42.043606 50-59
ACGGT 8308345 3.4570465 84.62891 40-44
AGGAG 8007190 3.4417267 81.833115 35-39
CTGAG 8228190 3.4236948 94.36448 35-39
ATGGT 8104295 3.4219449 22.304155 190-199
GGATG 8968935 3.4085267 70.53831 20-24
TAGTA 6258870 3.3209019 23.806093 80-89
GCACT 7283415 3.318109 86.31923 25-29
GCCCC 8480520 3.3172147 69.49945 60-69
TGTCC 8170680 3.2911177 18.456621 120-129
GAGGA 7508990 3.2275853 106.37925 35-39
TACAC 5548630 3.1764624 23.13013 110-119
CGGAT 7583705 3.1555288 78.39367 20-24
GGTTG 9385910 3.153783 77.50984 15-19
GATGC 7566885 3.1485302 95.28886 25-29
CCTGG 8555925 3.1018462 27.756615 50-59
AGGCT 7384935 3.0728219 18.41764 140-149
CCAGG 7484510 3.0689304 38.519802 50-59
CAGAT 5851305 3.059467 15.5137205 150-159
CCACT 6123910 3.0545645 22.465452 240-247
CTCTC 6912870 3.0486562 12.4815235 100-109
AGGGT 8012500 3.045046 48.74544 50-59
GTTGG 9006120 3.026169 55.65291 15-19
TCAGG 7267145 3.0238104 19.506163 140-149
TGGTG 8903265 2.9916084 16.980122 190-199
CCGTG 8197045 2.9717383 20.504574 120-129
AGATA 4938010 2.9633467 15.226197 150-159
TGGCC 8104050 2.9380243 42.675114 60-69
TCTTG 7140485 2.918636 15.404152 170-179
CTTGG 7853470 2.8892283 13.454699 170-179
TTCCC 6438115 2.8392837 44.687737 50-59
GCGGA 7522660 2.8172815 91.5969 20-24
CTTCA 5534275 2.8012266 13.573721 200-209
TCGCA 6146295 2.800071 19.789873 100-109
CCCTT 6337355 2.7948472 16.160826 20-24
GGTTC 7595650 2.7943788 46.246464 50-59
TCTCA 5518045 2.7930117 18.766811 130-139
CGGTG 8404935 2.783061 66.19436 40-44
GGCGG 9310510 2.7747886 49.28002 20-24
CGCAC 6075630 2.7275949 20.901937 100-109
TCAAA 4113870 2.7029994 42.642666 70-79
CCAGT 5922480 2.698107 35.410717 60-69
GCCGT 7387565 2.6782713 19.466785 120-129
AGTCA 5090745 2.6617937 27.62418 70-79
TTGGG 7830445 2.6311274 83.29145 15-19
TGTCT 6368270 2.602997 16.776514 180-189
GGCTG 7806055 2.5847585 12.95686 140-149
TCTCT 5729515 2.5641 12.697293 180-189
GTTCC 6354955 2.5597508 50.958622 50-59
CGGCC 7152245 2.5552216 11.134999 120-129
CAGCT 5566430 2.5359013 11.916197 150-159
ACAGC 4865390 2.5069382 10.891755 120-129
TCCAT 4937300 2.4990623 13.881736 70-79
CTGGC 6774020 2.4558382 34.08202 60-69
TTCTT 5362345 2.435224 16.851622 170-179
TGGGG 8050185 2.43461 58.582294 15-19
TGTTC 5950085 2.432066 12.175475 150-159
TCCTC 5462780 2.4091496 15.927948 130-139
GATGG 6320235 2.4019232 12.31377 190-199
GCCCT 6047030 2.4002707 15.130184 200-209
TCAGC 5262060 2.3972392 13.51498 140-149
ACCAC 4227905 2.3851523 13.516161 230-239
AGATG 4922430 2.350757 14.790743 180-189
TACCA 4092785 2.3430247 14.3888445 230-239
AACCA 3557480 2.3034077 12.20289 70-79
GGGGC 7556550 2.2520604 55.665543 15-19
AAAAA 2638555 2.2504504 5.854782 9
GCTGT 6100945 2.2444885 9.908236 140-149
GAGAT 4641790 2.2167344 14.279239 180-189
CACGG 5383120 2.2072816 9.503249 120-129
GGGCG 7404935 2.2068746 68.78612 20-24
GACTC 4833915 2.2021892 15.033521 190-199
CATTT 4232285 2.1738496 14.702539 150-159
TCATT 4188150 2.1511803 14.637594 150-159
GTCAA 4100505 2.1440275 32.953846 70-79
CTGGA 5141135 2.1391919 11.994465 180-189
CAGGC 5211075 2.1367366 15.820834 140-149
GTTCT 5173575 2.1146717 15.153417 160-169
TGAAT 3972590 2.1078215 12.435456 190-199
TTCAT 4091235 2.1014013 14.068535 140-149
ACTAC 3644800 2.0865636 11.703758 230-239
TAGTC 4507595 2.0838552 26.13439 70-79
ATTTG 4428440 2.0774972 13.294511 150-159
CTCGC 5196515 2.0626729 14.964972 100-109
AGCTC 4512985 2.0559828 12.118116 140-149
TCTGC 5095050 2.052266 11.368885 210-219
TTGTC 4966935 2.0302086 12.565742 170-179
TGGAG 5341755 2.0300643 11.851383 180-189
CAGCC 4501295 2.0208125 10.530646 120-129
CTACT 3986125 2.0176156 8.123062 230-239
GTCCT 5005055 2.0160162 13.954717 130-139
ATCTC 3958730 2.0037494 6.543281 100-109
ACAGA 3383990 2.0012093 8.365057 140-149
CAAAA 2692405 2.0008113 15.698033 70-79
TGGAA 4146885 1.9803873 8.6644745 170-179
GATAC 3754355 1.9630365 12.714366 150-159
GTTCA 4178585 1.9317544 12.782205 140-149
TACAG 3693700 1.931322 12.616152 160-169
TCGGC 5315490 1.927066 7.61566 190-199
CTCTG 4774685 1.9232242 12.9550495 180-189
ACCAT 3334060 1.9086721 9.8143425 45-49
GAATC 3620340 1.8929642 12.805285 190-199
TTGGA 4479215 1.8912967 13.317772 170-179
AATAA 2498895 1.8844339 6.7236586 80-89
TTCAC 3709030 1.8773613 15.016975 200-209
TTTGC 4583685 1.8735573 11.246113 150-159
TCTCG 4642390 1.8699362 14.110038 100-109
TTGCA 4035935 1.8658074 12.312708 150-159
ACGGC 4534630 1.8593688 12.406427 120-129
ACTCT 3662990 1.8540577 11.045616 200-209
CCTTC 4194450 1.8498013 11.964007 200-209
GTGAA 3834660 1.8312811 14.139259 190-199
AGGGA 4248635 1.8261887 5.7186985 150-159
ACACA 2815820 1.8231953 12.069674 120-129
AATCG 3482210 1.8207405 12.381841 190-199
TCACA 3167105 1.8130944 7.2920036 140-149
CTGTT 4399205 1.7981521 11.566491 140-149
CGTAG 4317640 1.7965411 9.171197 80-89
TACTA 3083990 1.7915871 9.777784 240-247
TGTAG 4199575 1.773222 5.885139 210-219
GCAGA 3760195 1.7695853 12.61489 150-159
AATAT 2653815 1.769429 7.764027 110-119
CCATA 3067910 1.7563075 16.025558 70-79
CATAG 3348555 1.7508563 7.1503916 210-219
ACACG 3347685 1.7249264 12.019896 120-129
CACTA 2998860 1.716778 11.723401 240-247
GATAT 3205650 1.70089 6.058778 180-189
TGCTC 4219540 1.6996139 6.2940664 150-159
GTCTC 4216710 1.698474 10.403479 180-189
TGCAG 4081030 1.6980892 12.03541 150-159
AGTAT 3143100 1.6677015 10.999408 220-229
CTCCA 3329870 1.6609164 7.955321 150-159
TGGTT 4433325 1.655072 17.74779 10-14
GAGTC 3946720 1.6422037 10.674399 210-219
ATATG 3080675 1.6345793 6.13835 180-189
ATTGT 3479050 1.6321136 7.006455 170-179
CTGTG 4418925 1.6256871 5.595991 210-219
CACCA 2881545 1.6256098 9.317977 230-239
TCAGA 3089475 1.6153913 8.884951 130-139
GAACT 3088245 1.6147481 7.1092353 160-169
GTGCT 4387055 1.6139624 7.0789356 220-229
CTGCT 4000980 1.611579 5.9475236 140-149
CAAAG 2694285 1.5933344 22.743221 70-79
GCTCT 3946555 1.5896567 9.647852 130-139
CCAGA 3066560 1.5800742 20.028902 60-69
CTGGT 4293515 1.5795498 5.551292 160-169
TGTGC 4290535 1.5784535 7.7962775 220-229
CAGAG 3344620 1.5740116 6.3164086 140-149
TCTGG 4262730 1.5682241 10.315855 180-189
TTCAG 3379620 1.5623938 7.200953 150-159
GCTCC 3845845 1.526546 7.211773 150-159
GCTCA 3305785 1.506018 9.579795 140-149
GTCTG 4088605 1.5041649 8.436039 210-219
CCATC 3001610 1.4971825 12.507686 240-247
AGTCT 3194970 1.4770304 11.02912 210-219
ATAGT 2773185 1.4714277 6.685886 220-229
CCTCA 2945165 1.4690282 8.384988 130-139
GACGT 3481580 1.4486622 6.8432007 60-69
GGTCA 3471760 1.4445761 5.0070896 130-139
TGACT 3123335 1.4439136 9.591236 190-199
ATAAC 2189825 1.4388144 7.379858 80-89
ACTTC 2814465 1.4245687 6.7212915 210-219
ACCGT 3110170 1.4169017 15.174976 45-49
ATGTA 2669565 1.4164479 6.5313377 160-169
GGCCG 4309950 1.4063505 10.66266 120-129
GAACC 2727245 1.4052389 10.599982 70-79
ATACC 2446000 1.4002784 11.660216 70-79
GTCAC 3062430 1.3951527 5.874254 140-149
GTCCC 3514310 1.3949486 17.250584 50-59
CCACC 2837570 1.3947617 7.3951893 230-239
TGATA 2618285 1.3892392 5.775945 180-189
AGCCG 3385905 1.3883483 9.551262 120-129
CCTTG 3442245 1.3865227 10.724608 20-24
GTTGT 3689935 1.3775457 5.0218153 210-219
ATATA 2057780 1.372023 7.1728535 240-247
GCGTA 3297340 1.3720012 9.434578 210-219
GAGAA 2534285 1.3688442 6.9877787 160-169
AACTG 2600560 1.3597528 6.907881 160-169
CTGAT 2916250 1.3481785 6.195338 180-189
CCTCG 3392900 1.3467569 7.9574356 130-139
ACTGA 2567170 1.3422941 6.06155 180-189
TTGGT 3581535 1.3370773 11.747064 25-29
GGACG 3556655 1.3319886 5.016894 170-179
AGAAC 2244150 1.3271357 7.2601438 160-169
ATCAT 2270080 1.3187611 5.565181 230-239
ATCGG 3149515 1.310492 7.927479 190-199
GTAGG 3426885 1.3023429 5.729312 160-169
TAGTT 2768425 1.2987406 8.578074 220-229
CATCA 2267060 1.2978394 7.0668607 230-239
AGCTT 2803710 1.2961514 5.1787233 150-159
AATTG 2434970 1.291974 8.336577 170-179
CAATA 1960100 1.2878746 5.509361 100-109
GTGTT 3430390 1.2806511 7.0654635 160-169
TATGG 3013845 1.2725613 5.0452743 190-199
CTCTT 2825100 1.2643023 6.4054694 200-209
AATAG 2096830 1.2583277 6.535696 240-247
TCCCA 2520240 1.2570785 9.027161 240-247
CAGCG 3063525 1.2561604 7.024826 160-169
TTGGC 3408880 1.2540997 8.185405 60-69
AAAAG 1840330 1.2490985 11.1343155 70-79
TTCCA 2415740 1.2227501 7.8658004 230-239
GAATT 2303240 1.222079 8.9905205 170-179
ACGGA 2587230 1.2175763 8.264796 200-209
CTTCT 2719565 1.2170727 6.2027836 210-219
TCACG 2666410 1.2147378 8.64709 200-209
TGGTC 3295145 1.2122575 10.514616 50-59
AGATC 2312490 1.2091298 6.7817893 130-139
TATCA 2071730 1.2035334 6.1329045 70-79
TTCTG 2918665 1.1929891 5.9134073 210-219
AAAGT 1983830 1.1905154 15.953684 70-79
GGCTC 3273435 1.1867439 7.2112722 130-139
GCTGA 2841665 1.1823977 9.320549 30-34
CTTGA 2557190 1.1821854 6.099538 200-209
TCTGT 2886660 1.1799072 6.6238275 210-219
GATCT 2532610 1.1708223 5.71734 130-139
TCCAC 2344030 1.1691862 7.881705 240-247
GGAAT 2436950 1.1637903 8.061284 170-179
ATATC 1998985 1.1612735 6.2973714 70-79
TTGTA 2452340 1.1504569 5.8095546 220-229
CCATG 2494505 1.136423 6.641283 150-159
CCAGC 2530395 1.1359961 6.3220997 80-89
AGCGT 2728670 1.1353813 6.7715373 160-169
AGGTT 2687105 1.134599 22.024529 10-14
GAGGG 3310425 1.1323421 5.6365037 200-209
AGAGC 2404900 1.13177 5.5250163 140-149
CATAC 1968470 1.1269035 14.458235 70-79
CAGAC 2184890 1.1257854 11.74523 60-69
AAGAG 2084170 1.1257235 10.003928 35-39
TGGAC 2704570 1.1253535 7.558206 170-179
GCATA 2149430 1.1238707 6.736452 220-229
GGAGT 2921965 1.1104547 6.554864 210-219
GTGGT 3288125 1.1048511 9.463547 50-59
CCCAC 2228190 1.0952308 8.638026 240-247
CTGCC 2752325 1.092491 6.439933 200-209
GTCCA 2382195 1.0852578 13.363528 70-79
CGAAC 2104815 1.084526 10.944347 70-79
AACTT 1862920 1.082229 5.699961 210-219
GAAGA 1999540 1.0800123 13.001602 35-39
CGGCT 2974090 1.0782199 6.422159 130-139
TGCGT 2920330 1.0743659 7.713881 210-219
TGCCC 2693245 1.0690401 5.3933573 200-209
TTGAG 2530325 1.0684005 5.802585 200-209
ATCCA 1865600 1.0680128 12.311104 240-247
AAGTA 1770645 1.062581 12.235909 70-79
ATAGA 1759865 1.0561117 6.9281864 240-247
TACTG 2281275 1.0546303 5.114114 180-189
CTCGG 2899240 1.051084 7.3535504 130-139
ACGTG 2497280 1.0391015 6.244218 170-179
CTACC 2073195 1.0340955 7.149102 230-239
AGCTG 2456835 1.0222726 5.3878083 90-99
GTGGA 2659250 1.0106133 9.895375 25-29
CTGAA 1931665 1.0100081 11.347973 35-39
ATCAA 1536625 1.0096323 9.938211 70-79
CGACT 2207720 1.0057721 5.8985662 200-209
ACTGG 2410085 1.0028203 5.216486 160-169
TAACC 1738770 0.9954056 5.6274548 80-89
TAGGC 2385385 0.9925427 6.681665 160-169
TATTT 1899785 0.99020714 5.555445 220-229
GAGCT 2369755 0.98603916 5.0159073 140-149
TATAA 1476780 0.98464173 6.888505 240-247
CTGCG 2712135 0.98325145 6.2474213 210-219
GTCTA 2115755 0.9781107 5.4886503 180-189
CTTCC 2198705 0.96965456 6.356124 230-239
AAAGC 1628215 0.9628868 8.197852 70-79
ACAAT 1464295 0.9621082 6.287997 100-109
GGAGG 2811195 0.9615787 7.764522 30-34
CATGT 2066175 0.9551899 6.2324414 150-159
TCCCC 2184580 0.9494039 5.205231 90-99
GGGGT 3120050 0.94359374 8.316395 10-14
CCATT 1855870 0.9393665 5.0778575 50-59
CGGAG 2506150 0.9385682 6.4394264 210-219
AGAGA 1736940 0.93817407 9.540859 35-39
AAGCA 1574305 0.93100566 5.5303435 70-79
ATGAT 1751280 0.9292138 8.674437 240-247
TATGT 1976160 0.9270685 5.458515 220-229
CGTGG 2771070 0.9175629 6.774441 50-59
TCTAC 1809355 0.9158225 5.524574 180-189
ACTCG 1988675 0.9059817 5.86772 190-199
CTCGA 1985775 0.90466064 5.4654436 190-199
TCGAC 1968035 0.8965787 5.9883738 190-199
AAAGG 1637740 0.8845931 7.356846 10-14
AGCCA 1711635 0.8819362 6.2061133 80-89
GTATT 1843735 0.86494434 5.213612 220-229
TGCAT 1814795 0.8389773 5.5157537 210-219
AAGGT 1727910 0.8251811 9.033316 10-14
TGAAG 1705190 0.81433094 7.4912577 35-39
ACTAA 1225985 0.8055278 8.862701 240-247
TGTAA 1515610 0.8041695 5.7161646 230-239
ACGTC 1754515 0.79930526 9.196393 70-79
CGTCC 2005480 0.79604286 8.313903 70-79
CCGTA 1745760 0.79531676 6.9020915 80-89
CCAAT 1389235 0.79530483 6.661613 240-247
TAGAT 1487065 0.78902364 5.614574 240-247
CAATC 1356905 0.77679664 6.387 240-247
TATGA 1463120 0.7763187 6.7467036 240-247
TCGAA 1483055 0.7754438 12.892236 70-79
ATGGG 1987440 0.75530064 15.091519 15-19
GCGTG 2275275 0.7533942 5.1041293 160-169
GACCG 1826065 0.7487553 14.456706 45-49
CCCAA 1320320 0.7448522 6.858664 240-247
ACCCA 1320295 0.7448381 9.394971 240-247
CTAAT 1265305 0.7350556 7.246477 240-247
TACCG 1596340 0.72724545 8.431493 70-79
AATCC 1248860 0.7149435 6.1975455 240-247
GAGGC 1900120 0.71160626 6.1046233 30-34
GAAGG 1654265 0.71105194 5.0087442 10-14
GGTGG 2290470 0.6927047 11.06378 25-29
AATGG 1437720 0.68659794 5.7664127 10-14
CATCC 1358575 0.6776479 7.5165067 240-247
GGTCC 1816035 0.65838134 10.610572 50-59
GGGTC 1920475 0.635912 5.389477 70-79
TAGGT 1467550 0.619656 5.1869373 10-14
GAGGT 1616285 0.61424804 6.842584 10-14
CGGTT 1653615 0.6083517 8.510219 10-14
GTCGA 1381515 0.5748391 9.706289 70-79
GGTCG 1722020 0.57019913 5.440531 70-79
GGGCC 1588880 0.51845664 8.618937 15-19
AAGGG 1186320 0.50991535 5.824388 10-14
TGGGC 1523355 0.50441676 9.118761 15-19
TAAGG 1040020 0.49667218 5.105179 10-14
TGATC 934580 0.43205506 5.5074425 240-247
TGGGT 1285830 0.43205497 5.24686 10-14
CGATG 1029355 0.4283077 6.490213 15-19
GATCC 846630 0.38569966 5.635953 240-247