FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_5_SL248351.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_5_SL248351.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11480369
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 54757 0.47696202099427293 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 41962 0.3655108995189963 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 39972 0.3481769619077575 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 38776 0.33775917829818886 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 37033 0.32257673947588267 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 36833 0.32083463519334615 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 36578 0.31861345223311205 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 36132 0.31472855968305546 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 35059 0.30538217020724684 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 32678 0.28464241872364904 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 32444 0.28260415671308126 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 32364 0.28190731500006666 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 31426 0.27373684591497016 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 31143 0.2712717683551809 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 31132 0.2711759526196414 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30981 0.2698606638863263 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 30939 0.2694948219869936 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30328 0.2641726934038444 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 29985 0.26118498455929423 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29558 0.25746559191607865 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 28536 0.24856343903231684 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 28400 0.24737880812019195 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27580 0.24023618056179205 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27515 0.23966999666996763 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27366 0.23837212897947793 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27213 0.23703941920333746 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26855 0.233921052537597 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26816 0.23358134220250237 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26545 0.23122079089966532 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25834 0.22502761017524786 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25776 0.22452239993331224 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24242 0.2111604600862568 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23808 0.20738009379315248 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23696 0.206404515394932 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23194 0.2020318336457652 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23055 0.2008210711694023 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 22426 0.19534215320082482 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22404 0.19515052172974579 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22154 0.19297289137657508 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22124 0.1927115757341946 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 21990 0.19154436586489512 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21962 0.19130047126534 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 20714 0.18042974054231184 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 20691 0.18022939854982012 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 20283 0.17667550581344554 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20268 0.1765448479922553 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20138 0.17541248020860656 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 19996 0.17417558616800558 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19293 0.16805208961488954 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19280 0.16793885283652468 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18763 0.16343551326616768 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 18256 0.15901927890993747 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18197 0.15850535814658917 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 17480 0.1522599142936956 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 17433 0.15185051978729952 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17389 0.15146725684514148 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 16877 0.14700746988184787 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 16799 0.14632804921165862 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 16760 0.14598833887656398 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16311 0.1420773147622694 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16069 0.13996936858040016 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15880 0.1383230800334031 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15579 0.13570121308818558 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15184 0.13226055713017587 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14516 0.12644192882650374 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13178 0.11478725117633415 No Hit
ATGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 13125 0.11432559354146196 No Hit
TAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 12980 0.11306256793662293 No Hit
AAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 12525 0.10909928069385226 No Hit
TTGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 12300 0.10713941337599862 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 12034 0.104822414680225 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12032 0.10480499363739962 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 23462080 7.713591 67.28754 35-39
TATTA 11733435 7.5217543 130.38855 240-247
TGGAT 17837950 7.2979927 81.135345 35-39
TCACC 18304200 6.5095034 76.17917 160-169
TCTCC 16187450 6.1543717 87.09868 20-24
CTGTG 17048070 5.992024 87.84381 25-29
TCCCT 15306215 5.8193316 72.71141 15-19
AGTGG 18403250 5.8174286 39.868908 100-109
CTGAG 17420395 5.727276 50.473713 15-19
TGTAT 10672345 5.6511936 60.002563 240-247
TTACT 9934255 5.47103 115.46332 240-247
ATTAC 9948520 5.124884 108.50924 240-247
TGGGT 14694590 4.965949 46.12949 100-109
CCTGA 14399890 4.9238267 51.924374 15-19
TTCAC 10743975 4.75478 76.83557 40-44
CTCTG 12966720 4.740039 73.9212 35-39
ACACG 14660285 4.6889734 49.90166 230-239
CTCCA 13128475 4.668865 41.81517 80-89
GACAC 14540020 4.650507 46.84644 230-239
TGAGA 11879930 4.546363 96.22171 15-19
TACTG 10337945 4.3989215 81.352715 240-247
ATTCA 8512170 4.3849626 114.51501 40-44
AAGGG 14821310 4.382429 39.789734 90-99
TCTGG 12252285 4.3064103 136.45038 35-39
AGCCT 12367715 4.2289543 52.53601 30-34
GAAGG 14261890 4.2170177 31.78204 150-159
TGGAG 13249660 4.188334 39.49914 90-99
CCCTG 14243850 4.184184 85.9028 10-14
CAGCC 15171505 4.1687307 56.605255 30-34
ACCAT 10060270 4.1645384 69.87988 160-169
CCAGG 15713640 4.1514378 53.86579 80-89
TCAGT 9647990 4.1053367 99.29434 7
GAGAC 13300675 4.0903034 89.218124 8
CACCA 12175120 4.050066 59.09554 160-169
ACTAC 9753715 4.0376368 36.78072 130-139
TCCAG 11786450 4.0302 41.247013 80-89
GTATT 7576900 4.012101 70.01687 240-247
TACTA 7756975 3.9959314 30.174582 130-139
GGGTC 14619275 3.9701073 128.85712 8
ACTGT 9084270 3.8654668 70.25787 240-247
CACCT 10721875 3.813009 45.49541 40-44
CTGGG 14034880 3.8114052 136.4519 3
TGAAG 9929165 3.7998195 40.750885 150-159
GACTC 11071540 3.7857466 52.065926 20-24
AGACT 9471860 3.7699795 70.68853 15-19
GATTC 8804930 3.7466044 59.542194 40-44
CAAGA 9936545 3.6993968 45.646667 180-189
GGACA 11867450 3.6495492 28.914518 220-229
GCTGG 13224025 3.591204 33.78205 90-99
ACTGG 10833185 3.5616083 44.91792 60-69
CCTGT 9561930 3.495404 61.350933 25-29
ACTCT 7833010 3.4665232 68.769325 20-24
GAGTG 10741915 3.3956134 39.99305 90-99
TATCT 6137760 3.3802104 50.117954 200-209
TCCTG 9177405 3.3548388 65.0134 20-24
AGCTG 10147460 3.3361638 38.27868 60-69
AGACA 8959420 3.335611 39.788506 170-179
GGATT 8119680 3.3219826 56.88099 40-44
CTCCT 8650095 3.2887144 94.66032 20-24
CTGCA 9451745 3.2318828 45.812336 25-29
GGTCC 11426225 3.22725 143.77994 9
CATCT 7157710 3.1676672 32.772633 170-179
GTCTC 8584665 3.1381607 33.65505 30-34
CCATC 8800930 3.1298654 35.537376 45-49
GTCCC 10386595 3.0511012 75.272095 10-14
CCTCA 8556165 3.0428195 83.65284 5
AAGAA 6936460 3.0060315 39.51419 180-189
GGAGT 9460440 2.9905283 37.90947 90-99
AGTAG 7775720 2.9757116 29.53214 50-59
GGCTG 10840410 2.9438934 34.90119 90-99
GAGCT 8893840 2.9240136 31.005695 60-69
AGGGA 9883040 2.9222605 41.462837 80-89
TGTGC 8139460 2.8608422 58.63197 25-29
ATCTG 6622175 2.817816 37.5481 200-209
GTGAA 7340700 2.8092327 39.99588 10-14
CAGGG 10978720 2.7888162 37.05635 80-89
GACAA 7488800 2.7880962 25.034023 180-189
GGAAG 9420285 2.7854307 31.302147 80-89
CTATG 6493945 2.7632525 28.95331 50-59
ACCTT 6224825 2.754816 74.36194 40-44
GAACA 7354895 2.738243 35.640026 210-219
ATCTC 6181310 2.7355583 48.723045 170-179
GTGTA 6661345 2.7253382 37.58527 230-239
ATGAA 5776375 2.6762042 27.895424 210-219
GGGAA 8905110 2.6331015 34.755077 80-89
AGAAC 6970260 2.5950425 30.724121 190-199
TGCAA 6515810 2.5934155 36.732174 200-209
CACGG 9815980 2.5933156 44.740997 230-239
GAGTC 7880345 2.5908086 28.908695 150-159
AGGGC 10074300 2.5590754 33.6012 150-159
ATCAG 6410030 2.5513134 27.794537 45-49
CGCCA 9251780 2.542146 40.04173 70-79
CAGTG 7707055 2.5338356 76.64402 8
CCCTC 8237945 2.516841 26.810644 20-24
TATAT 3917875 2.5115654 25.516432 240-247
AGGCT 7635990 2.5104723 21.059591 25-29
CCAAG 7828185 2.5037816 40.077103 180-189
CTACA 6026420 2.4946902 20.208235 190-199
ACGGC 9364185 2.4739542 49.546635 230-239
GTGGA 7706865 2.4362078 27.042606 100-109
CACTG 7045595 2.4091358 19.619108 30-34
GCAGC 9110380 2.4069006 34.63845 30-34
TCTGT 5277685 2.4008424 25.24803 210-219
CTGTC 6503015 2.3772047 49.482845 30-34
CTACT 5346980 2.366323 23.560701 50-59
TGAAC 5923865 2.3578103 38.4505 210-219
TATGC 5539260 2.3570223 21.992308 50-59
CTCTC 6161315 2.3424952 98.4085 20-24
CCGCC 9897745 2.336422 34.196083 70-79
CAAAT 4848225 2.3361468 36.49762 200-209
ACAGC 7303275 2.3358932 27.621815 210-219
GGGCT 8593550 2.3337216 43.11781 90-99
CTTCA 5252530 2.3245237 51.36776 45-49
TCCAA 5569980 2.3057427 44.229305 180-189
GTGGT 6818300 2.304204 32.738632 120-129
GTATC 5411345 2.3025928 23.154154 200-209
GGCCG 10486845 2.2885103 25.780834 230-239
CAGGC 8624755 2.278602 33.948116 70-79
TGGTG 6693805 2.262132 34.87955 35-39
GCCTC 7667025 2.2522173 72.70811 4
AAATG 4839135 2.2419794 34.68058 200-209
AATGA 4825980 2.2358844 26.251842 210-219
ACAAC 5762325 2.2312422 20.433256 180-189
CAGTA 5592480 2.2259126 22.062672 45-49
GCTCC 7557390 2.2200117 34.27384 80-89
TACAT 4279720 2.2046568 18.999777 190-199
GTAGT 5364640 2.1948206 26.619812 120-129
GCCTG 7740740 2.1863134 31.327835 210-219
GCTAT 5114860 2.176435 35.052814 50-59
GGGGG 10773110 2.1734123 97.63026 5
TATCA 4188650 2.1577427 17.557566 170-179
CCTTC 5606055 2.1313887 55.702244 45-49
GTGGG 8156565 2.1297581 30.423697 100-109
GCTGT 5967540 2.0974598 19.616867 230-239
CCTCT 5501955 2.0918105 49.32012 35-39
GCAGA 6800990 2.0914812 25.005442 140-149
AACTA 4339810 2.0911639 38.458145 130-139
TGCAG 6352595 2.0885327 43.895996 30-34
TCTAT 3789880 2.087177 20.603535 110-119
AACAG 5516630 2.053853 34.416004 210-219
TGGTA 4984175 2.0391622 32.573887 120-129
TGCAC 5942910 2.032089 26.931376 25-29
AGCAC 6351350 2.0314276 21.453716 130-139
CAGAG 6589595 2.0264716 33.141613 170-179
TGAGC 6145140 2.020328 18.384907 200-209
ATGGA 5272870 2.017889 12.93129 100-109
CTCAC 5601480 1.9920481 54.69168 20-24
ATATC 3860625 1.9887638 14.241662 160-169
TGTGA 4852550 1.9853106 19.579138 210-219
AGAGT 5166780 1.9772891 31.5925 150-159
GGCTC 6998505 1.9766743 20.325735 40-44
CATAT 3800840 1.9579662 15.041436 160-169
GGTCT 5555580 1.9526647 36.439976 15-19
CCGTG 6904850 1.9502224 20.666424 230-239
TCCGC 6636720 1.9495614 42.32512 70-79
GCACA 6088605 1.9473906 19.355694 130-139
CTGTA 4571665 1.9452989 25.18931 190-199
TTCAG 4543345 1.9332484 47.850193 45-49
ACTAT 3752380 1.9330025 18.123476 130-139
CAGAC 6019640 1.9253329 27.080906 140-149
TCTCT 4020120 1.9020094 32.772224 35-39
TGGGG 7262500 1.8963091 127.08088 4
CTATT 3437895 1.8933307 20.895506 110-119
TCCAT 4266260 1.8880467 27.093504 45-49
ACCAG 5900345 1.8871776 14.070247 190-199
GGATC 5736500 1.88598 18.20749 70-79
CCAGA 5887980 1.8832226 34.72435 170-179
GCCAG 7121105 1.8813479 35.793037 70-79
GGTGG 7197785 1.8794111 18.560608 40-44
GTCAC 5494630 1.8788065 38.738297 160-169
GATGG 5928660 1.8741014 21.638134 100-109
AGAGA 5203930 1.862831 28.960659 170-179
GCTGA 5644290 1.8556643 28.13852 200-209
AGGAC 6031410 1.8548155 21.199068 220-229
CAGTT 4337725 1.8457545 25.301445 190-199
CCTGC 6262040 1.8394977 50.229317 20-24
ACCCT 5171930 1.839288 43.261505 10-14
TGGGA 5811800 1.8371611 17.162657 100-109
CACAG 5736375 1.8347331 16.392376 140-149
TAGTA 3693050 1.8291833 17.993801 120-129
ATACT 3550580 1.8290471 27.877 130-139
TTCCA 4125505 1.8257552 23.590416 180-189
TAGTG 4447855 1.8197389 27.093494 120-129
TCAGC 5308265 1.8150817 11.617026 45-49
GAGGA 6130600 1.8127226 27.615574 220-229
GCCGA 6855895 1.8112812 20.035694 220-229
TGTCT 3970190 1.8060571 50.94186 30-34
CAAGG 5841010 1.7962623 17.842358 80-89
AGTCA 4464285 1.7768699 29.811508 160-169
CATGA 4460205 1.7752458 24.319223 60-69
TACAA 3675460 1.771043 18.480585 130-139
AGCTA 4418795 1.7587639 26.84436 50-59
GCAAA 4720015 1.7572715 33.252407 200-209
AGTTC 4103030 1.745889 22.55844 190-199
TTCTG 3804110 1.7305065 43.879734 30-34
TGACC 5049270 1.7265223 14.636552 210-219
CTCAG 5022265 1.7172884 79.99329 6
ACCTG 5017645 1.7157086 30.418127 25-29
GGAGC 6724030 1.7080392 13.607323 200-209
ATGCA 4288400 1.7068642 16.500515 60-69
GTGCA 5188000 1.7056507 51.6327 25-29
CTGAA 4256460 1.6941515 23.85931 200-209
GGATG 5353395 1.692255 23.101633 100-109
AACAC 4358155 1.6875305 22.999079 190-199
CCTGG 5964450 1.6846137 35.155033 2
ATTGG 4048220 1.6562374 21.27773 100-109
CAGCT 4785285 1.6362562 13.559305 50-59
AGGGG 6672095 1.6295847 33.48854 90-99
TCTGC 4432970 1.6204909 34.008842 200-209
GCCGT 5686270 1.6060438 30.136755 230-239
CGATT 3753335 1.5970895 35.39534 160-169
GGGGC 7607285 1.5961887 28.967222 90-99
GGGCC 7287695 1.5903703 28.27246 2
TCTCA 3581395 1.5849576 27.27822 100-109
ACAAT 3284350 1.582584 23.879715 180-189
TCTGA 3674995 1.563755 21.719992 220-229
CTCCC 5108815 1.5608352 14.04519 190-199
AGTTA 3133120 1.5518476 16.716125 50-59
CCATG 4533240 1.5500735 16.256748 160-169
GGCTT 4402895 1.5475211 22.305042 25-29
GTTCT 3393455 1.5436977 23.791065 190-199
AGTGA 4021540 1.5390141 88.89085 9
GCACT 4495880 1.5372986 25.968063 25-29
TTAGT 2893925 1.5323839 26.668896 110-119
CGTCC 5210715 1.5306671 19.254917 180-189
CTTTA 2772120 1.5266724 43.938683 45-49
GAGCA 4941445 1.5196227 7.868949 200-209
TTCTC 3202330 1.5150946 22.63713 190-199
CGGCC 6663305 1.5123461 31.923773 230-239
GTTAC 3548760 1.5100404 16.666103 50-59
GTCCG 5296825 1.4960481 34.542786 70-79
CATCA 3611175 1.4948779 32.097214 45-49
ACTCC 4084465 1.4525537 17.523123 140-149
TAGCA 3639990 1.4487848 12.38809 130-139
TACGC 4186600 1.4315451 25.162354 140-149
CTGGT 4052580 1.4243933 31.928156 35-39
GGGGT 5413080 1.4134074 121.64835 7
GTGAC 4284285 1.4085377 15.564968 210-219
AAGCT 3536835 1.4077272 17.54308 200-209
CTCAA 3399880 1.4074105 18.871817 150-159
CAACT 3393575 1.4048005 18.316362 130-139
GGCAG 5519375 1.4020325 13.975648 150-159
GTCCA 4079405 1.3948915 29.201622 180-189
CAACC 4161335 1.3842723 15.392712 140-149
AACCC 4116925 1.3694993 20.609158 140-149
AGCAG 4447225 1.3676372 13.0843315 50-59
GAGAG 4612875 1.3639549 26.371546 220-229
CTACG 3987470 1.3634557 16.646744 130-139
CGACA 4247560 1.3585474 16.76762 70-79
CGTGT 3864395 1.3582504 24.705894 230-239
TTACA 2618610 1.3489517 9.977935 120-129
GAACT 3379800 1.3452243 14.9077425 190-199
CCTTT 2840240 1.3437816 50.133587 45-49
ATCTA 2600840 1.3397979 21.138878 110-119
CAATT 2599875 1.3393006 25.064493 180-189
CCATA 3228390 1.3364208 15.463571 160-169
CACGT 3907655 1.3361641 16.799223 180-189
GGCCT 4703810 1.3285552 69.70014 3
GTGCG 4879725 1.3251708 17.596004 240-247
ACAGT 3307905 1.3166088 12.738759 120-129
ATACA 2726260 1.3136649 22.450544 35-39
TGGAA 3427305 1.3116047 19.67507 120-129
TCAAG 3292010 1.3102823 23.902977 150-159
TGCGA 3974535 1.3067015 14.757998 240-247
AATTC 2535860 1.3063239 25.040956 180-189
ACAGA 3506280 1.3053955 20.690918 140-149
ACATG 3276060 1.3039339 14.884296 190-199
AAGAG 3625940 1.2979639 19.596489 150-159
ACATA 2685875 1.2942052 22.018444 130-139
GTGGC 4764320 1.2938305 14.446921 40-44
TTATT 1882835 1.2903706 20.251093 240-247
TGTCC 3525485 1.288756 54.12335 15-19
ATATT 2009635 1.2882825 27.059366 240-247
TCCGT 3516950 1.285636 13.8685465 150-159
AGAGC 4179465 1.2852942 25.338312 220-229
TGTGT 2935855 1.2841072 14.9271345 240-247
AACCA 3305030 1.2797477 17.644777 190-199
CCAGT 3738220 1.2782284 19.302916 190-199
TCACT 2858930 1.2652285 13.733183 190-199
ACGTC 3645395 1.246488 18.63915 180-189
GATTG 3040440 1.243927 24.364834 100-109
ACACA 3199280 1.2388 7.926339 130-139
AGACC 3864845 1.2361393 100.75045 9
ATCCG 3597750 1.2301967 24.25792 70-79
CTTCT 2599460 1.2298632 23.89587 30-34
CCTAC 3455190 1.2287654 17.490473 190-199
CGGCT 4333655 1.2240078 21.323946 230-239
ATTAT 1903795 1.2204335 9.328922 50-59
AGTCG 3695505 1.2149653 16.573015 150-159
TGGAC 3679575 1.2097281 16.359379 80-89
ATTAG 2431785 1.2044735 26.268991 110-119
TACCA 2906785 1.2032895 11.552879 45-49
TGAGT 2926035 1.1971207 21.533203 90-99
ACGCA 3693050 1.1811919 21.752575 140-149
GTCGA 3584680 1.1785296 12.911231 150-159
GATCC 3430505 1.1730099 24.062275 70-79
ATTCC 2645680 1.170854 23.022528 180-189
ATCAT 2272590 1.1707029 16.055647 110-119
GAAGT 3029430 1.1593409 13.885612 140-149
AGCTC 3388400 1.1586124 12.574527 210-219
TCGGA 3514830 1.1555651 95.55818 5
CTTCG 3159880 1.1551074 106.96741 3
GGGTG 4411830 1.1519713 14.83765 60-69
AAGGC 3711045 1.1412429 29.710213 25-29
CGCTG 4033695 1.1392865 12.672783 190-199
GTATA 2298450 1.138432 10.6313 240-247
GGACT 3454770 1.1358193 13.30755 90-99
GATCT 2669195 1.1357749 9.7156725 220-229
TCGAG 3448890 1.1338861 12.591259 150-159
AGGCC 4260810 1.1256771 14.719529 2
GCAAG 3659590 1.125419 17.327131 25-29
TTGGG 3329675 1.1252438 15.890206 100-109
ACAAG 3019400 1.124129 18.159697 80-89
GAACC 3484570 1.1145114 10.968413 180-189
GAAGC 3612185 1.1108408 13.620785 200-209
CACAT 2683215 1.1107408 14.63394 130-139
GCCCC 4689620 1.107013 12.321343 80-89
TTCGG 3137390 1.102724 102.8668 4
CCCCT 3608390 1.1024283 9.919633 70-79
CTTGA 2587530 1.1010255 22.750751 90-99
CGCAG 4160625 1.0992091 17.04888 140-149
GCTTC 3006030 1.0988669 27.717329 2
TTGAG 2684625 1.0983534 21.24905 90-99
GACCC 3992805 1.0971178 42.358814 10-14
CCGAT 3199540 1.0940346 23.699135 160-169
AGGTC 3325515 1.0933243 18.056974 10-14
TAGAC 2733010 1.0877897 18.149183 170-179
ATCAA 2248390 1.0834005 13.07157 110-119
CTATA 2082735 1.072901 14.001301 50-59
ATGGG 3386900 1.0706288 20.206179 100-109
ATGGT 2607080 1.0666275 10.124622 35-39
GCACC 3877570 1.0654542 11.227329 130-139
GAGCC 4018840 1.0617503 18.596113 220-229
ATGAG 2774275 1.0616949 20.899876 60-69
ACACC 3182385 1.0586236 13.603715 40-44
CTCCG 3578330 1.0511477 10.158114 140-149
AGATC 2634800 1.0487002 13.066885 210-219
GGAGA 3512990 1.0387362 85.634995 7
GTAGC 3147290 1.0347296 11.42562 50-59
TAGTT 1954070 1.0347142 15.463268 50-59
GCTCT 2826585 1.0332701 17.770098 210-219
CGTGA 3139505 1.03217 21.019888 150-159
TACAG 2592185 1.0317388 9.149778 120-129
CAGCA 3219860 1.029846 12.37849 45-49
TGGCT 2917940 1.0255922 31.769867 40-44
GACAG 3312725 1.018749 16.160475 70-79
AAACT 2110205 1.0168151 17.927814 130-139
AGCCG 3833570 1.0128032 19.64703 220-229
GGGAG 4142095 1.0116603 13.797881 5
TTTAG 1907665 1.0101421 31.898672 45-49
CGAGG 3976515 1.010115 15.092122 220-229
ACCGC 3652660 1.003655 11.173122 220-229
TGAGG 3163335 0.9999579 11.847074 220-229
ATGAC 2508750 0.99853 11.458554 160-169
GTAGA 2604950 0.99689543 17.79953 170-179
TTTCA 1800690 0.99168277 7.410315 100-109
GCCAA 3094800 0.98984665 15.11101 180-189
ACCAA 2544365 0.98520905 17.686083 130-139
AAGGT 2573075 0.9846971 21.263073 15-19
ACTCA 2376090 0.98360354 12.734204 190-199
AACTC 2375190 0.983231 16.573002 190-199
CGAGT 2980455 0.9798795 15.119201 160-169
GGTAT 2387315 0.9767157 9.474397 110-119
TTATA 1520145 0.9744936 13.444788 110-119
CCAAC 2913710 0.9692486 14.599734 130-139
CCGAG 3655855 0.96585214 18.498402 220-229
CATAC 2314440 0.958083 14.851363 130-139
TAACA 1963035 0.9459004 9.133948 120-129
CAGAA 2538525 0.9450982 18.743063 140-149
GGGAC 3720155 0.94499433 10.187071 90-99
TATGG 2302195 0.94189084 13.0205345 35-39
TACAC 2274680 0.94162405 23.138313 35-39
ACAGG 3060170 0.94108176 15.703855 70-79
CCGTC 3201250 0.9403791 10.248443 140-149
GATAC 2359490 0.93912166 24.312796 35-39
GCCAT 2734290 0.9349495 7.946694 60-69
AATCA 1938530 0.93409246 14.633371 110-119
ATGAT 1883755 0.9330319 8.94497 120-129
AGCCC 3383735 0.9297614 11.56725 80-89
TGGTT 2119095 0.92686635 9.615719 50-59
CGGAC 3502050 0.92521787 10.890156 220-229
AACTG 2309655 0.9192864 11.954605 60-69
TCATA 1782970 0.9184799 9.094135 110-119
GCAGT 2785770 0.91587317 6.145648 50-59
AAGTT 1841755 0.9122291 17.977049 140-149
TTTAT 1329460 0.911124 10.743108 240-247
GAGAT 2364385 0.90483296 12.572424 210-219
CCCAG 3267015 0.8976898 14.928762 80-89
CCCCA 3106610 0.8878006 16.94404 80-89
GACTG 2699390 0.887474 12.956062 90-99
GGTAG 2801540 0.8855913 12.671006 120-129
GTTAT 1669735 0.8841539 10.810898 110-119
AGAAG 2460295 0.8807024 16.562216 140-149
TCCTT 1851810 0.876133 11.435303 1
CGTAT 2044260 0.86985743 7.8267236 240-247
CGGAG 3417450 0.86810124 73.77074 6
TATTT 1265525 0.8673071 10.519136 240-247
ATAGT 1721055 0.85244584 15.84893 120-129
ATCCA 2058635 0.85219026 10.056945 180-189
GTTTC 1865900 0.8488062 5.1993413 100-109
GATCA 2127975 0.8469743 9.411308 110-119
TTACC 1911185 0.84580094 9.998755 45-49
CATGG 2570850 0.8452141 8.841888 200-209
ACGCT 2463900 0.84249353 15.647215 190-199
GGTTC 2388985 0.839676 11.866813 160-169
GCCTA 2455245 0.8395343 16.790085 190-199
TCTTC 1763575 0.8343871 47.657143 2
GATGA 2168460 0.82985383 6.6522417 60-69
GTCTA 1948155 0.8289636 10.588897 30-34
GCTTG 2349550 0.82581544 17.445158 90-99
AGTCT 1932995 0.82251287 14.584913 8
ACAAA 1818820 0.81978285 13.61766 130-139
ATATG 1641170 0.81287855 6.1001577 50-59
TGGCA 2455920 0.807429 5.6660986 100-109
ACATC 1949695 0.80709356 5.82421 110-119
GGTGC 2970875 0.80679053 11.825651 60-69
CCTCC 2628050 0.8029167 10.705056 140-149
TCATC 1813025 0.80236 10.24693 110-119
CTATC 1809470 0.8007867 8.010873 110-119
GTCTG 2272550 0.79875165 14.025683 1
ATATA 1320435 0.7917768 11.165047 130-139
TATAC 1532740 0.7895763 9.3318615 130-139
GTTCA 1828775 0.77816594 14.424983 160-169
TTATG 1467010 0.77680755 11.349924 50-59
GGTTA 1886115 0.7716611 8.602729 50-59
CATTA 1497315 0.77132744 12.542159 110-119
TGATG 1844490 0.7546312 11.756666 120-129
CCAGC 2744250 0.7540478 6.1354547 80-89
CTGAC 2194105 0.75024134 9.924413 220-229
GGGAT 2369440 0.7490007 13.782934 100-109
TCAAC 1788485 0.7403593 7.857258 110-119
CCCCC 2996340 0.73563033 13.48544 80-89
CATCC 2067410 0.73523074 6.524766 110-119
GCGGA 2870405 0.72914076 9.2757015 220-229
TTTAC 1320810 0.72740144 13.641565 45-49
GACCG 2753075 0.72734374 9.554108 210-219
TGACT 1708275 0.72689176 5.4836764 2
AACGC 2267955 0.725387 14.78124 180-189
GGGCA 2825285 0.7176794 13.984778 150-159
ATCTT 1302740 0.71744996 13.360606 1
ACGCC 2604900 0.715758 13.160849 180-189
CACAA 1839265 0.71218574 8.618863 130-139
ATGCC 2081295 0.71166766 8.510833 50-59
GACCA 2220595 0.7102392 6.3645062 170-179
TTGGA 1733505 0.70922416 6.3404775 60-69
TGCCA 2074085 0.7092023 7.6377807 60-69
ATAAA 1262805 0.7082944 7.822399 130-139
TATTG 1335160 0.70699066 6.488868 240-247
CAACG 2207280 0.70598066 15.03474 180-189
GATTA 1418170 0.7024256 5.5122366 50-59
TCCGG 2479255 0.70024675 11.784474 70-79
GGATA 1829655 0.70019567 15.777593 35-39
CAAAC 1792255 0.6939829 14.3751 130-139
GAAAT 1497745 0.69390774 7.5655313 110-119
CGCCG 3047180 0.6916074 11.015238 220-229
AATAC 1414850 0.6817541 11.410751 130-139
TTTCT 1149315 0.6766766 9.0580635 4
TATAG 1365275 0.67622656 6.2613873 120-129
GCCGC 2899995 0.6582014 12.098487 220-229
GCGAC 2487725 0.6572401 7.392076 70-79
CACGC 2389420 0.6565499 12.593409 190-199
AAATA 1166435 0.65424144 12.675752 130-139
GACGA 2109015 0.6485769 10.225334 220-229
CCCGT 2179870 0.6403449 10.021787 140-149
CAGTC 1871720 0.6400065 5.309203 210-219
TCAAT 1235495 0.6364534 8.767273 110-119
TCCAC 1789375 0.63635343 10.5447645 180-189
AGGCA 2067975 0.63595605 6.4611683 80-89
TACTT 1129620 0.62210864 11.500183 1
AATAA 1107765 0.621334 7.253568 130-139
CCGGC 2726435 0.61880904 9.540345 70-79
AGATG 1615260 0.61814827 5.897218 120-129
AAGCA 1659430 0.6178093 6.0832696 120-129
ACTTC 1390950 0.6155693 29.971897 4
CCCTA 1706005 0.60670465 11.99875 110-119
TAGCT 1422885 0.60545486 12.554955 50-59
CACGA 1890865 0.6047778 9.091702 180-189
CACCG 2198755 0.60416013 9.331192 45-49
AATGG 1569100 0.6004831 7.011733 120-129
GGCCC 2632725 0.59754 10.525991 70-79
ACGAC 1856545 0.59380084 9.54903 220-229
TTAGC 1395080 0.59362346 10.46016 45-49
AGTAA 1259325 0.58344734 5.107215 50-59
CGGTT 1635915 0.57498837 11.746235 160-169
CTTTG 1262080 0.57412577 6.9882874 45-49
CCGTA 1663375 0.56876606 6.755412 230-239
GTTCC 1551845 0.5672835 6.7239943 140-149
ACCCG 2064050 0.5671467 8.773209 140-149
ACCTA 1356750 0.5616387 7.1067786 130-139
GGGGA 2283025 0.5576033 8.482907 4
TGATT 1050175 0.55608606 5.95473 50-59
CGGCA 2082230 0.55011106 11.0186825 70-79
TCAGG 1668600 0.548583 5.5376825 150-159
ATTTC 994365 0.5476204 6.2811794 240-247
TAATA 907895 0.5444041 7.8944955 130-139
CTGCG 1919485 0.542144 12.064725 25-29
GGTGA 1712835 0.54144216 6.243529 120-129
TTCAA 1047160 0.5394344 5.41805 200-209
CCACG 1956425 0.5375742 7.9378257 180-189
ATTAA 894675 0.5364769 6.264275 110-119
GCTAC 1549365 0.5297822 8.046048 50-59
AAATC 1090625 0.5255243 8.089345 110-119
GTCCT 1426475 0.521454 6.533177 45-49
CTGTT 1142445 0.51970327 5.761774 240-247
CCTAT 1169255 0.5174575 5.0249257 110-119
ACCCC 1809160 0.51701796 9.2550335 140-149
TATGT 975110 0.5163378 6.883742 140-149
GAAGA 1438275 0.51485384 5.979323 15-19
GGAGG 2101745 0.5133277 5.4187546 6
AAGAT 1098755 0.509055 5.4833465 15-19
ACATT 984525 0.5071686 6.269044 110-119
GTAAC 1252830 0.49865004 7.2928853 120-129
TGACG 1510275 0.4965307 9.043661 220-229
AGTTT 937025 0.49617115 5.1761627 140-149
GGGTA 1561610 0.4936386 5.2775946 100-109
CGCGG 2196765 0.47939298 6.5743895 220-229
GGGTT 1416235 0.4786082 5.776072 100-109
CCGGT 1673925 0.47278738 9.119773 160-169
AGTAT 949120 0.4701032 5.0883045 110-119
TGGCC 1652090 0.4666204 15.136961 2
CAATC 1123725 0.46517593 6.5811133 110-119
TATCC 1043515 0.46181086 5.272721 110-119
AACAT 958075 0.46165428 5.9532475 110-119
CCATT 1037060 0.4589542 5.0991993 110-119
CATAA 948330 0.4569586 6.122305 110-119
GGTTT 1035275 0.45281667 5.4988317 100-109
CCGCG 1950605 0.44272172 6.8054214 220-229
TAAAT 736865 0.44184878 7.835771 130-139
ATGCT 1035320 0.44054124 6.8773856 50-59
TGCTA 1015330 0.43203524 6.174607 50-59
TGCGC 1509220 0.42626774 9.08716 25-29
ATTCT 772640 0.4255112 7.6741266 2
GGTCA 1290880 0.42440048 5.353613 160-169
GCGCT 1495520 0.42239824 8.291734 25-29
TTGGT 952775 0.41673216 5.122766 120-129
GCATG 1261135 0.41462126 8.390652 60-69
GCCTT 1125610 0.41147152 8.412464 1
ACGAG 1330835 0.40926632 8.001936 180-189
CTAAC 975765 0.4039266 5.6818376 110-119
CCTAA 961900 0.39818704 7.869271 110-119
CAATG 996940 0.39680097 5.278209 120-129
ATGGC 1206580 0.3966854 7.062415 1
GGTAA 1015895 0.38877562 7.609258 120-129
CACTT 865570 0.38306075 6.6812224 3
AGCTT 892615 0.3798185 7.488051 1
TTTGA 713830 0.37798548 6.0622907 45-49
CCGGG 1731665 0.37789568 5.1214304 80-89
CATGC 1098085 0.37547365 6.8062263 60-69
GAGGC 1430470 0.3633682 5.642043 35-39
TTCTT 612875 0.36083943 20.245924 1
CGAGC 1309005 0.34583026 6.7467036 180-189
TGGGC 1257930 0.34161183 9.4602375 1
ATGTG 824840 0.33746457 5.193466 140-149
TGCTG 942390 0.33122948 14.023077 1
CGCTT 852560 0.31165692 6.136969 1
AACCT 744330 0.30812204 5.0658493 2
CTCTT 648085 0.3066236 8.694315 1
GCGAG 1186755 0.3014597 5.5136547 240-247
AACTT 558920 0.28792226 10.680348 3
GACTT 669310 0.28479952 6.8337607 1
ATTGT 524725 0.27785107 5.29445 240-247
TTCCT 583580 0.27610484 6.2539344 4
TAACT 507725 0.26154965 6.8480663 2
CCCTT 681485 0.25909653 5.523306 3
GAGGT 818365 0.25869238 6.841094 7
GTCTT 553060 0.25158945 12.175216 1
AATCT 478625 0.24655907 7.523138 2
TTGGC 687980 0.24180993 7.3469315 1
TGTAA 485110 0.24027704 7.3410425 25-29
CGGGG 1132205 0.23756346 8.468815 3
CCTCG 754260 0.22156668 5.125095 5
TGCTT 458920 0.20876475 10.946054 1
TCGGT 540370 0.18992823 5.680703 7
TCGCA 454925 0.15555479 5.0546975 7
TAGGC 466365 0.15332608 7.1038656 1
TTGCT 261230 0.11883468 5.5462065 4
Produced by FastQC (version 0.10.1)