FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_5_SL248351.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_5_SL248351.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11480369
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 13352100 8.832193 63.102753 110-119
ATACA 12210320 7.6554475 39.004314 110-119
CACAG 17841990 7.5887213 43.104008 110-119
TCCCT 21000185 7.1303554 109.54911 30-34
CAGTA 15147135 6.797219 40.500214 60-69
AGTAA 11565915 6.48223 49.046104 110-119
GGAGA 18471800 6.2782173 96.35407 35-39
CCCTG 21071670 6.061951 71.89303 30-34
GTAAT 12620180 5.9750533 40.614304 110-119
GGTGA 20573785 5.907084 102.56081 45-49
AATAC 8845640 5.5459104 40.891823 110-119
ACAGT 11847775 5.3166447 36.2968 110-119
GTAGT 15685660 5.315404 27.690697 80-89
AGACG 13847630 5.2650404 103.68337 40-44
GTGAC 15757680 5.0611634 85.486435 45-49
GAGAC 12600825 4.790989 143.62129 40-44
CCCCA 12556560 4.7835627 102.61374 60-69
ACTCC 11823625 4.7523327 123.70618 30-34
CTCAG 13118190 4.713364 35.418365 140-149
ACCAG 10890650 4.6321125 96.88592 45-49
TAGTA 9587235 4.5390987 35.380783 80-89
CACTC 11022790 4.430449 101.74782 25-29
GCACA 10357740 4.4054503 34.751656 110-119
GGCCC 18033685 4.3956523 59.750233 60-69
CGTGT 15957210 4.329591 23.636179 120-129
GTGTC 15928210 4.321722 24.0715 120-129
TGACC 11980840 4.304715 145.88734 45-49
AGTAG 10567255 4.239004 28.643648 80-89
CTCCC 13139570 4.2285714 71.17163 30-34
ATGCA 9175505 4.117473 99.281425 25-29
GACCA 9519005 4.048712 109.37903 45-49
CCCAG 11802870 4.0194745 87.795 60-69
TGAGG 13319205 3.8241706 70.33617 35-39
CCACT 9501270 3.8188963 28.552423 240-247
AGGAG 11102155 3.7734134 96.98164 35-39
TACAC 7488430 3.7591586 28.469654 110-119
ATGGT 11022940 3.7353466 25.859097 190-199
CAGGG 13648050 3.7141044 36.650143 50-59
ACGGT 11485970 3.6891448 98.03395 40-44
GACGG 13523990 3.6803434 109.826584 40-44
AGATA 6550520 3.671303 20.161293 150-159
CTGAG 11414255 3.6661108 106.6251 35-39
TGCAC 10010325 3.5967093 119.19385 25-29
GAGGA 10477070 3.5609589 127.056854 35-39
TGTCC 11638125 3.5324166 20.69533 120-129
CCCTT 10378960 3.5240488 33.256306 20-24
GGGTT 14407660 3.4944932 43.304356 50-59
CCTGA 9636240 3.4623 87.343025 30-34
GCCCC 12620100 3.441128 73.45998 60-69
TGGTG 14106330 3.4214075 18.001144 190-199
GGATG 11760455 3.376627 72.109764 20-24
CCAGG 11017290 3.3539557 41.50015 50-59
GCACT 9310045 3.3450985 92.33383 25-29
TCTTG 10372750 3.321684 18.058624 170-179
CCGTG 12785355 3.2879648 23.818478 120-129
CTCTC 9677580 3.2859035 13.866286 100-109
AGGCT 10220270 3.2826183 23.443386 30-34
CAGAT 7299130 3.275457 18.367376 150-159
CTTCA 7672985 3.2538385 17.171513 200-209
TCAGG 10079485 3.2373996 21.220707 140-149
CTTGG 11917970 3.2336435 18.894333 20-24
CCTGG 12440110 3.1991792 30.149672 50-59
TCGCA 8902745 3.1987555 25.007122 100-109
TGTCT 9798875 3.1379108 19.846434 180-189
TCTCA 7399385 3.1378145 22.336222 130-139
CGCAC 9139395 3.1124263 25.529934 100-109
CGGAT 9605665 3.085215 81.956436 20-24
GGTTG 12661115 3.0708787 74.90797 15-19
TTCCC 9024405 3.0641263 51.045498 50-59
ACAGA 5696135 3.0258632 14.388019 140-149
ACCAC 6358625 3.0254335 20.56344 230-239
GATGC 9275200 2.979074 98.4279 25-29
GGTTC 10896635 2.9565294 48.877766 50-59
AGGGT 10250810 2.9431822 51.473324 50-59
TGGCC 11374620 2.9251711 43.937523 60-69
GTTGG 11813670 2.8653362 54.146053 15-19
GACTC 7957360 2.8590786 19.892296 190-199
TCAAA 4536875 2.844463 59.215565 70-79
GCCCT 9847870 2.83306 17.973618 20-24
TCTCT 7886840 2.8253124 15.075695 180-189
CGGTG 11961540 2.749808 68.86839 40-44
CGGCC 11266660 2.7462122 13.056056 120-129
CCAGT 7638350 2.7444587 37.28957 60-69
TTCTT 7245725 2.738551 20.459806 170-179
AGTCA 6097135 2.736066 32.8216 70-79
GCCGT 10604740 2.7271838 22.477373 120-129
GATGG 9482945 2.7227151 18.659145 15-19
GTTCC 8895010 2.6998234 55.344048 50-59
TCACA 5377350 2.6994057 11.533405 140-149
AGATG 6603340 2.6488986 17.464838 180-189
GCGGA 9437415 2.5682454 90.042786 20-24
TCCAT 6047265 2.564429 17.174362 70-79
AGGGA 7484630 2.543885 9.275805 150-159
TGGAG 8674460 2.4905853 15.925374 25-29
TACCA 4952205 2.4859848 17.068121 230-239
TGTTC 7757480 2.4841917 13.173365 140-149
TCCTC 7298265 2.4780362 16.976091 130-139
ACAGC 5822690 2.4765608 11.291846 120-129
GGCTG 10729450 2.4665658 12.086553 30-34
GGCGG 12581605 2.4506245 43.31293 20-24
ACTCT 5742375 2.435136 13.467482 190-199
GAGAT 5993645 2.4043224 17.105043 180-189
TTGGG 9871870 2.394364 81.43872 15-19
CAGCT 6609865 2.3749242 12.429332 150-159
GTTCT 7398050 2.3690906 18.063808 160-169
CTGGC 9188700 2.3630254 35.536102 60-69
CACGG 7734360 2.3545446 9.778086 120-129
CTGGA 7309545 2.347731 14.91037 180-189
ACTAC 4612640 2.3155248 13.503222 230-239
TGTAG 6817850 2.3103669 8.229391 210-219
AATAA 2944985 2.3060584 9.84952 110-119
CTACT 5377850 2.2805538 8.067115 230-239
CATTT 5080885 2.2732465 17.821815 150-159
TCATT 5057775 2.2629068 16.542078 150-159
CACTA 4473470 2.245662 15.515124 240-247
GTGAA 5588135 2.2416542 18.244493 190-199
TTCAT 4990940 2.233004 16.428896 140-149
CAGAG 5839165 2.2201228 9.488955 140-149
TCAGC 6118730 2.1984591 14.953426 140-149
GATAC 4899105 2.1984549 15.096728 150-159
TTCAC 5181185 2.1971555 18.770533 200-209
TTGTC 6700430 2.1456907 13.540054 170-179
ATTTG 5339635 2.1355987 15.68005 150-159
TGGGG 10354840 2.1279411 53.83226 15-19
ACACA 3568125 2.1203527 14.24093 110-119
TAGTC 5573865 2.1129491 27.139656 70-79
ATATA 3178595 2.102588 10.990296 110-119
CTCGC 7292320 2.0978727 16.781044 100-109
TTGGA 6132520 2.0781288 15.899653 170-179
TCTGC 6840365 2.0761952 12.084964 210-219
ATGTA 4381050 2.0742183 7.5677114 70-79
TACAG 4612910 2.070026 14.112224 160-169
CTCTG 6814940 2.068478 14.761015 180-189
AGCTC 5730100 2.0588243 12.775728 140-149
GAATC 4561140 2.0467942 14.512344 190-199
GCTGT 7536405 2.0448155 10.305068 140-149
GTCTC 6708965 2.0363128 11.97417 180-189
GTCAA 4531030 2.0332825 41.524963 70-79
GAACT 4523470 2.02989 10.887179 160-169
GTCCT 6666170 2.0233235 14.22377 130-139
CAGGC 6642400 2.022123 15.623841 140-149
TCTCG 6661170 2.0218058 16.140919 100-109
AATAT 3031960 2.0055916 13.606802 110-119
GTTCA 5272665 1.9987696 15.307708 140-149
TGAAT 4220495 1.9982032 13.929281 190-199
ATCTC 4708545 1.9967257 7.832023 100-109
AACCA 3357435 1.9951506 15.846196 70-79
TACTA 3762205 1.9925895 10.779814 230-239
AATCG 4430255 1.9880602 13.46769 190-199
GATAT 4180705 1.9793644 8.550266 180-189
ACGGC 6499425 1.9785976 14.703821 120-129
TTGCA 5200400 1.9713753 14.467165 150-159
ACACG 4586920 1.9509516 13.60482 120-129
TGGAA 4858570 1.9489926 9.483335 170-179
CCTTC 5714365 1.9402424 14.095901 200-209
ATATG 4088755 1.9358304 8.831903 180-189
GAGTC 6008185 1.9297512 12.371205 210-219
TTTGC 6021035 1.9281268 12.200438 150-159
CATAG 4276735 1.9191686 8.679559 210-219
CAGCC 5604140 1.9084932 10.281798 120-129
GGGGC 9752040 1.8994863 49.183334 15-19
TCGGC 7369690 1.8952371 8.1452265 130-139
AGTAT 3932695 1.8619435 12.259967 220-229
GGGCG 9498740 1.8501489 60.240147 20-24
GACGT 5605565 1.8004348 9.940447 170-179
CTGTT 5582015 1.7875384 12.902147 140-149
AGAAC 3361160 1.785493 11.515478 160-169
GTTGT 6227445 1.7826875 7.0100584 210-219
CGTAG 5531010 1.7764888 10.022436 210-219
GCAGA 4620855 1.7569063 14.965551 150-159
CCAGA 4123805 1.7539752 26.517973 60-69
ATTGT 4358375 1.7431415 7.713146 170-179
CACCA 3641665 1.7327039 13.816447 230-239
AGTCT 4558820 1.7281644 13.188264 210-219
CTCCA 4260295 1.7123631 8.014808 150-159
ATAGT 3595860 1.7024683 7.6033545 220-229
TGCAG 5298970 1.7019606 13.661224 150-159
GGTCA 5277245 1.6949828 7.793912 130-139
TGACT 4438530 1.6825647 11.842485 190-199
GTCAC 4656345 1.6730245 8.791437 130-139
TCCCA 4160505 1.672254 10.951779 240-247
AACTG 3725515 1.6718109 10.838245 160-169
GAGAA 3483015 1.653961 10.5564575 160-169
TGGTT 5750450 1.6461414 18.301653 10-14
TCAGA 3666235 1.645209 10.4353895 130-139
TGTGC 6044895 1.6401312 8.31319 220-229
TAGTT 4084225 1.6334947 10.155181 220-229
CAAAA 2199820 1.6326717 21.602127 70-79
AATAG 2911515 1.6317872 9.809618 240-247
ACTGA 3625665 1.6270034 9.071493 180-189
CCTTG 5326720 1.6167719 21.530544 20-24
GCTCC 5612810 1.6147074 6.5097027 150-159
GAACC 3791080 1.6124575 12.976962 70-79
GTGCT 5932960 1.6097606 8.364991 220-229
TTGTA 4014195 1.6054862 9.200325 210-219
CTGTG 5913385 1.6044493 5.4240904 210-219
GTCTG 5884550 1.5966258 9.117282 210-219
CAAAG 3001445 1.5944077 29.855436 70-79
TCTGG 5875710 1.5942272 12.033101 180-189
TGCTC 5246220 1.5923387 5.3171663 150-159
ATAGA 2816010 1.5782603 10.062819 240-247
CTGGT 5806450 1.5754352 7.0867553 160-169
TTCAG 4135220 1.5675855 9.344254 150-159
GCTCA 4348980 1.5625882 11.502692 140-149
GGCCG 7160490 1.5602082 13.330515 120-129
GTCCC 5408000 1.5557871 19.669992 50-59
AAAAA 1675235 1.5528557 8.331732 8
CCACC 4065785 1.5489062 10.072451 230-239
GGACG 5688015 1.5479047 7.3565097 170-179
TTGGT 5401885 1.5463601 22.832241 25-29
ACCAT 3065375 1.5388047 11.537683 45-49
CCCAC 4012405 1.5285708 10.728325 240-247
GCTCT 4903160 1.4882127 10.312884 130-139
CCTCG 5105765 1.4688394 9.6713085 130-139
TATAC 2750335 1.4566693 7.131923 110-119
TGATA 3031595 1.4353156 8.041484 180-189
CACTG 3983415 1.4312406 7.353112 230-239
AAATA 1820925 1.4258677 5.4253364 9
CATGT 3760540 1.4255512 7.5167756 70-79
CGACT 3967320 1.4254578 8.808089 190-199
AATTG 2991605 1.4163823 9.592798 170-179
GCTGA 4366525 1.4024713 20.06736 30-34
CAGAC 3294005 1.4010369 15.596058 60-69
TGGAC 4358800 1.3999903 10.400595 170-179
TCGAC 3896240 1.3999187 9.463707 190-199
CTCTT 3855450 1.3811426 8.055985 200-209
GCGTA 4291355 1.3783276 10.5156555 210-219
GGGAG 5637415 1.3714007 6.649039 150-159
GAGGG 5633115 1.3703548 8.393883 200-209
CCATC 3406320 1.3691204 10.965805 240-247
ACGGA 3567155 1.3562765 9.567025 200-209
CCTCA 3362355 1.3514494 7.3792896 130-139
CTGAT 3548495 1.3451687 7.5347514 180-189
ACTTC 3135870 1.3298104 6.6109524 220-229
GAATT 2790860 1.3213391 10.597397 170-179
TCCAC 3283570 1.3197829 9.926442 240-247
TATGG 3888095 1.31756 6.3739367 180-189
CTCGA 3665875 1.3171486 8.485868 190-199
CTGCT 4336350 1.3161738 5.7605 90-99
AGATC 2919725 1.3102155 7.3068123 130-139
GTAGG 4553755 1.3074607 5.1831784 160-169
CCATG 3612325 1.2979081 7.648724 70-79
GTGGA 4509815 1.2948447 20.311327 25-29
CTTGA 3393045 1.2862405 8.43733 200-209
GTGTT 4485080 1.2839128 7.644174 160-169
ACGTG 3985705 1.2801569 9.544597 170-179
GATCT 3360565 1.273928 5.7730637 130-139
AGCCG 4171050 1.2697785 9.142429 120-129
GGCTC 4929150 1.2676121 8.696678 130-139
ACTAT 2392295 1.2670392 6.3619695 230-239
GGAGT 4404050 1.2644777 7.103372 210-219
ACCGT 3517845 1.2639616 18.311348 45-49
ATAAC 1997470 1.2523445 7.7617884 80-89
AAAGT 2221130 1.244854 21.883326 70-79
AAGTA 2219155 1.2437471 16.106367 70-79
GGAAT 3098560 1.2429727 8.850229 170-179
ATCGG 3859560 1.2396407 8.062885 190-199
ACTCG 3442135 1.2367587 8.655498 190-199
GGAGG 5070755 1.2335507 15.450262 30-34
GTCCA 3392485 1.2189196 16.077799 70-79
GCATA 2708560 1.215456 8.2232065 210-219
AGCTT 3200385 1.2132066 6.264177 150-159
GTGGT 4990565 1.2104323 10.365338 50-59
ACTGG 3767865 1.2101895 7.2583356 160-169
TTGAG 3526380 1.1949854 8.148459 200-209
TTGGC 4398025 1.1932943 7.914133 60-69
TTCCA 2799250 1.1870619 7.675343 230-239
TGGTC 4366310 1.1846892 11.738386 50-59
ATAAA 1508800 1.1814595 5.6750784 7
TGCCC 4080150 1.1737878 6.5972886 60-69
ATACC 2328430 1.1688615 7.4587636 70-79
ACTGT 3059975 1.1599799 5.6086745 230-239
TCACG 3222545 1.15786 9.675229 200-209
AGAGC 2997565 1.1397111 7.254328 140-149
ATCCA 2264640 1.1368392 16.126122 240-247
TCTAC 2669585 1.1320755 7.365609 180-189
TATCA 2133460 1.1299517 6.763992 70-79
TCTGT 3522285 1.1279474 6.5447083 210-219
CCATA 2238585 1.1237597 10.282278 70-79
AGGTT 3305260 1.1200544 23.98454 10-14
GTCTA 2929960 1.1106937 7.4321837 180-189
AGTTC 2929155 1.1103885 5.475349 160-169
GCTTC 3654130 1.1091057 5.590162 150-159
CTACC 2744470 1.1030995 8.994663 230-239
CAATA 1758785 1.1026974 6.545111 100-109
AAAAG 1654555 1.0977257 14.361994 70-79
TATGT 2744635 1.0977228 7.840001 220-229
TACTG 2891710 1.0961938 6.6792836 180-189
GAGCT 3399520 1.0918818 6.513335 140-149
CGGCT 4237230 1.0896735 8.07142 130-139
CTCGG 4212250 1.0832494 8.428479 130-139
CTGCG 4110620 1.0571136 7.091989 210-219
AGGGG 4335655 1.0547247 5.130767 60-69
CTGCC 3653630 1.0510854 5.82367 200-209
AACTT 1975140 1.0461001 6.0964355 200-209
GAAGA 2191925 1.0408678 17.393883 35-39
CATGG 3221785 1.0347956 5.4812784 190-199
ATACG 2295380 1.0300431 5.4938874 120-129
ATATC 1944345 1.0297899 7.285863 70-79
GGGGT 5003450 1.0282192 8.0131645 10-14
AGAGA 2149515 1.0207288 12.78818 35-39
TAGAT 2150165 1.0180006 7.7986465 240-247
GTCCG 3950065 1.0158243 5.009914 130-139
CTGAA 2263630 1.0157954 14.182082 35-39
TGCGT 3742145 1.0153377 8.401087 210-219
ATGGG 3522285 1.0113081 29.22622 15-19
GCGGT 4381010 1.0071393 5.8249755 130-139
TATAA 1506635 0.99661416 6.374232 240-247
ACCCA 2092725 0.9957184 13.3406925 240-247
CTTCT 2767580 0.99143356 5.5840783 210-219
TGCTA 2610660 0.989653 5.0706143 220-229
CGAAC 2318795 0.98625153 13.603742 70-79
ACTAA 1572015 0.9855989 13.0089445 240-247
AAAAT 1252260 0.98057705 5.0371594 8
CGGAG 3559325 0.9686149 6.6715016 210-219
CAGCG 3175205 0.96661675 6.465502 160-169
AGCTG 3000745 0.96380043 5.16335 90-99
TGTAA 2030955 0.9615603 7.022705 230-239
TCCCC 2978510 0.958543 5.080004 80-89
TAGGC 2925850 0.9397451 5.843366 160-169
AGTTT 2334975 0.93387836 5.0036526 220-229
ACAAT 1488315 0.9331219 7.574188 100-109
CCCAA 1959230 0.93220156 10.070316 240-247
CCATT 2193485 0.93017864 5.7218943 50-59
GAGTT 2730180 0.9251768 5.251519 160-169
TGCAT 2438560 0.924413 6.294036 210-219
CGTGG 3991680 0.9176372 7.1960187 50-59
TCGAA 2006280 0.90031046 17.021076 70-79
AAGAG 1885765 0.89548326 12.819378 35-39
TGAAG 2210640 0.8867878 9.389395 35-39
TTCTG 2724575 0.87249553 5.5041018 210-219
CCAAT 1732800 0.8698579 10.785652 240-247
CATCA 1725245 0.8660653 5.540054 230-239
TGAAC 1918810 0.8610587 6.8001504 190-199
CCAGC 2502230 0.8521359 5.8655415 80-89
GGTGG 4125545 0.847808 20.151995 25-29
CGGTC 3270870 0.84115815 5.6267567 130-139
CTTCC 2472430 0.8394832 5.404293 230-239
AGCGT 2608645 0.83786297 6.653682 160-169
GTATG 2470205 0.8370791 5.469251 220-229
ACGTC 2329540 0.83700347 10.978219 70-79
ATCAA 1325605 0.8311084 12.759088 70-79
AACCG 1923100 0.8179508 5.968007 190-199
GAGGC 3000705 0.8165951 13.065035 30-34
TAACC 1595980 0.8011749 6.18882 80-89
CTAAT 1470005 0.7785636 9.95142 240-247
GGGCC 3561270 0.77596956 15.331058 15-19
AAAGG 1609220 0.76416177 9.253182 10-14
CGTCC 2646105 0.76123816 8.99391 70-79
AAAGA 1146175 0.7604375 5.9240446 8
ATGAT 1591335 0.75342125 6.850598 240-247
TGAGA 1868610 0.7495841 5.809061 240-247
AGCCA 1755885 0.7468294 6.1025896 80-89
AATCC 1474085 0.73998415 9.734022 240-247
GACCG 2401375 0.7310424 16.865984 45-49
GGTCC 2836490 0.7294501 11.960023 50-59
GTCGA 2263675 0.72706306 12.192161 70-79
TGGGC 3147710 0.7236191 16.63758 15-19
TGTTA 1787645 0.71497256 5.0480804 230-239
GTAGC 2210750 0.71006423 5.53433 80-89
AAGGT 1764625 0.70787096 10.235858 10-14
CATAC 1406070 0.7058409 8.840144 70-79
AAAGC 1278030 0.6789066 9.851399 70-79
CATTG 1759110 0.6668461 5.2271533 50-59
TATGA 1403245 0.6643696 5.728569 240-247
CAATC 1303335 0.65426844 9.42678 240-247
TAGGT 1926080 0.65269125 5.594666 10-14
GAGGT 2205625 0.6332725 7.0360665 10-14
AGGAT 1567350 0.62873507 5.5874553 10-14
GGAAG 1842940 0.6263806 5.708497 30-34
ATAGG 1553350 0.623119 5.3591814 10-14
AGACC 1448715 0.61618096 5.214862 240-247
AGAAG 1274215 0.6050797 5.373247 9
CCGTA 1682305 0.6044521 5.1391435 80-89
CATCC 1465465 0.58902216 7.6151214 240-247
AATGG 1465830 0.58801085 6.621722 10-14
CGATG 1804055 0.5794391 12.833207 15-19
GGGTC 2478695 0.5698209 5.7553983 70-79
TAAAA 713480 0.55868757 5.95793 8
TGATC 1472650 0.5582544 5.549838 240-247
AAGAA 816965 0.5420209 5.3956656 9
GAAGG 1565290 0.53201264 5.5887747 10-14
AAGGG 1542790 0.5243653 6.473563 10-14
AAGCA 969620 0.5150751 6.5220947 70-79
GAAAA 775490 0.514504 6.2690473 8
GGTCG 2138760 0.4916741 5.859502 70-79
GATCC 1355260 0.48694482 5.527959 240-247
ATAAG 826695 0.4633293 5.391415 9
ACGAT 1006195 0.4515262 5.8892694 10-14
CGGTT 1600760 0.4343263 8.166053 10-14
TAAGG 1080005 0.4332389 5.91223 10-14
TAAAG 722355 0.40485093 5.9596972 9
AAGCT 879990 0.3948922 7.708072 30-34
TGGGT 1588655 0.38531896 5.1469116 10-14
Produced by FastQC (version 0.10.1)