FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_12_SL248341.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_12_SL248341.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9543351
Filtered Sequences 0
Sequence length 251
%GC 54

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 44454 0.4658112229132094 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 34914 0.3658463363654968 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 34252 0.358909569605058 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 32237 0.33779539283423615 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30960 0.3244143487963505 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30939 0.32419430030394986 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 30786 0.3225910898593167 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29225 0.30623415192420356 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 27933 0.292695930391746 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27815 0.2914594674344473 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26310 0.27568932547906916 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25375 0.26589192831742225 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24474 0.2564508001434716 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24467 0.25637745064600476 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23792 0.24930446339027035 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23603 0.2473240269586647 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23530 0.24655909648508162 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23282 0.24396042857482658 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23010 0.2411102766732566 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22977 0.24076448618519847 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22087 0.2314386215072672 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21740 0.2278025821328378 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21376 0.22398840826456032 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20944 0.2194616964208903 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20700 0.21690494250918782 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20551 0.21534364606310719 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20472 0.2145158445916953 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20191 0.21157138619338217 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20076 0.21036635873499782 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 19781 0.2072752013417509 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19580 0.2051690229144878 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19181 0.20098810155887592 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18630 0.19521444825826903 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18559 0.19447047478396215 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17743 0.18592001907925215 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17341 0.1817076622247259 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17309 0.18137235023630588 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17227 0.18051311326597963 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17002 0.1781554508474015 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16949 0.1776000903665809 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16868 0.17675133189589276 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 16831 0.17636362740928213 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 16770 0.1757244389313565 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 16220 0.16996126413038776 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16156 0.16929064015354775 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15623 0.1637055998464271 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 15588 0.16333885235909273 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 15123 0.15846635002736462 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15064 0.15784811854871522 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14472 0.1516448467629452 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 14233 0.14914048534943333 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14071 0.14744296840805707 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 13781 0.14440420351300082 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13580 0.14229802508573772 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 13475 0.14119778262373456 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13393 0.1403385456534083 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13392 0.1403280671537702 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13375 0.1401499326599221 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13306 0.13942691618489145 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13204 0.1383581092218027 No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG 12305 0.12893793804712833 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12225 0.12809965807607832 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11774 0.12337385473928392 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11630 0.12186495079139392 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11513 0.12063896633373329 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10903 0.11424708155447705 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10024 0.10503648037256516 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 9964 0.10440777039427765 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 9949 0.10425059289970577 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9753 0.10219680697063328 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 19271380 7.6392903 65.94205 35-39
TATTA 9574445 7.27379 126.156685 240-247
TGGAT 14619735 7.1808743 78.39633 35-39
TCACC 14895730 6.396875 73.97268 160-169
TCTCC 13162440 6.0784693 85.95501 20-24
CTGTG 13905500 5.927595 86.052505 25-29
TCCCT 12646855 5.84037 73.43241 15-19
AGTGG 15039735 5.7279267 39.73702 100-109
CTGAG 14074230 5.579109 49.411396 15-19
TGTAT 8711840 5.518591 59.550316 240-247
TTACT 8109500 5.346817 112.171715 240-247
ATTAC 8128705 4.983918 104.80193 240-247
TGGGT 11940400 4.890214 45.26888 100-109
CCTGA 11709090 4.831103 50.980488 15-19
CTCTG 10678400 4.737848 73.42961 35-39
TTCAC 8671565 4.6142507 73.9151 40-44
ACACG 11968660 4.5921593 48.796494 230-239
GACAC 11952765 4.5860605 46.138023 230-239
CTCCA 10605135 4.554307 40.559093 80-89
TGAGA 9604590 4.3869724 92.50778 15-19
TACTG 8453630 4.3217936 79.090515 240-247
TCTGG 10085360 4.299157 135.04344 35-39
ATTCA 6944015 4.257554 109.867386 40-44
AAGGG 12001125 4.2503777 39.041824 90-99
CCCTG 11682755 4.1833334 85.47818 10-14
TGGAG 10931385 4.16325 38.80242 90-99
CAGCC 12359525 4.1155453 54.8558 30-34
AGCCT 9971675 4.1142554 50.6298 30-34
GAAGG 11600585 4.10852 30.91536 90-99
CCAGG 12783375 4.089669 52.835033 80-89
ACCAT 8162375 4.03895 66.66795 160-169
TCAGT 7847080 4.011703 94.49682 7
GAGAC 10850530 3.9998107 88.877144 8
ACTAC 7994560 3.9559112 35.444897 130-139
CACCA 9859420 3.9373653 56.851307 160-169
GTATT 6210890 3.9343429 69.03526 240-247
TCCAG 9500845 3.9199939 40.06648 80-89
GGGTC 11757805 3.886312 124.16208 8
TACTA 6328825 3.8803658 28.366905 130-139
CACCT 8850380 3.8007386 44.99779 20-24
ACTGT 7396030 3.7811105 68.38433 240-247
CTGGG 11367840 3.7574162 131.32904 3
GACTC 8987840 3.7083309 51.26342 20-24
TGAAG 8105065 3.7020526 38.9465 150-159
GATTC 7177170 3.6692216 58.340794 40-44
AGACT 7666725 3.6448433 67.79059 15-19
GGACA 9785930 3.6073694 28.292185 220-229
CAAGA 8143455 3.6001945 43.68391 180-189
GCTGG 10819735 3.576251 33.058582 90-99
ACTGG 8914025 3.533573 44.298084 60-69
CCTGT 7909825 3.5094717 61.100777 25-29
GAGTG 9029630 3.4389608 38.83756 90-99
ACTCT 6397180 3.404021 67.28994 20-24
TCCTG 7470765 3.3146677 63.459026 20-24
TATCT 5018515 3.3088455 48.101543 200-209
GGATT 6656140 3.2693415 55.830795 40-44
AGCTG 8200860 3.25087 36.91943 60-69
CTCCT 7034760 3.2486813 92.90649 20-24
AGACA 7341710 3.2457457 37.573685 170-179
CTGCA 7776425 3.208508 46.235344 25-29
GGTCC 9324265 3.2078125 140.93515 9
CATCT 5898330 3.1385767 32.400425 170-179
GTCTC 7046005 3.1262076 33.908943 30-34
CCATC 7245850 3.1116836 35.640667 45-49
GTCCC 8583090 3.0734131 74.02402 10-14
CCTCA 7089080 3.0443597 79.96398 5
GGAGT 7875920 2.999567 37.123272 90-99
GGCTG 8861300 2.928929 34.195286 90-99
AAGAA 5725535 2.916616 37.076885 180-189
AGGGA 8227490 2.9138882 40.00234 80-89
AGTAG 6287800 2.8720024 28.623486 50-59
GTGTA 5826025 2.861608 36.344013 230-239
GAGCT 7193365 2.851493 29.835503 60-69
TGTGC 6592785 2.8103528 57.156204 25-29
ATCTC 5258740 2.7982428 47.3948 170-179
GGAAG 7813870 2.7673988 30.074003 80-89
GTGAA 6034620 2.7563603 38.29253 10-14
ATCTG 5346255 2.7331939 36.137997 200-209
CAGGG 8886425 2.7314093 36.19815 80-89
GACAA 6081900 2.688788 24.161806 180-189
ACCTT 5003475 2.662413 70.90188 40-44
CTATG 5193215 2.6549542 27.514292 50-59
GAACA 5988155 2.6473436 34.350414 210-219
GGGAA 7451265 2.6389768 33.69579 80-89
ATGAA 4725045 2.5883372 27.137972 210-219
GAGTC 6524040 2.5861688 28.38985 150-159
CCCTC 6920375 2.5792286 27.69547 20-24
CACGG 8020500 2.5659256 44.067703 230-239
CGCCA 7568920 2.520342 39.34452 70-79
AGAAC 5683340 2.5125859 29.970764 190-199
CAGTG 6320865 2.5056288 73.26665 8
ATCAG 5268465 2.5046852 27.46981 45-49
TGCAA 5262995 2.5020845 34.93839 200-209
AGGGC 8094070 2.487864 32.417477 150-159
CTACA 4973690 2.461108 19.37888 190-199
CCAAG 6384350 2.4495602 38.42946 180-189
ACGGC 7637310 2.4433353 48.819912 230-239
AGGCT 6154035 2.4394963 20.127848 25-29
GTGGA 6377880 2.4290342 27.051739 100-109
TATAT 3189485 2.4230797 23.791739 240-247
CTGTC 5441000 2.4140909 49.531013 30-34
TCTGT 4385610 2.411029 25.160532 210-219
CACTG 5783615 2.3862863 19.943409 30-34
CTACT 4452690 2.369333 23.248102 50-59
GCAGC 7379275 2.3607845 33.921055 30-34
CCGCC 8134300 2.3507144 33.82397 70-79
CTCTC 5059825 2.3366482 96.464905 20-24
TGAAC 4909095 2.3338368 37.3936 210-219
GTATC 4539410 2.3207061 22.605507 200-209
GTGGT 5645430 2.3120968 31.132029 120-129
ACAGC 6012790 2.3069992 26.469805 210-219
GGGCT 6961100 2.3008552 42.29262 90-99
TGGTG 5611385 2.2981536 35.02542 35-39
TATGC 4485660 2.2932272 21.205212 50-59
CTTCA 4302845 2.2895987 50.290962 45-49
TCCAA 4579330 2.2659686 42.39428 180-189
GGCCG 8469960 2.2594109 25.384518 230-239
CAAAT 3947715 2.2508347 34.457825 200-209
CAGGC 6970585 2.230036 32.9661 70-79
GCCTC 6195375 2.2184255 70.81884 4
ACAAC 4807905 2.2123616 19.64057 180-189
TATCA 3601875 2.2084022 17.119753 170-179
GCTCC 6124820 2.1931615 33.568806 80-89
CAGTA 4603370 2.1884918 21.301788 45-49
GCCTG 6346950 2.1835313 30.712387 210-219
AATGA 3979935 2.1801727 25.465431 210-219
AAATG 3955150 2.1665955 32.787292 200-209
TACAT 3511740 2.1531384 18.045782 190-199
GGGGG 8698900 2.1419647 93.90337 5
CCTTC 4623620 2.1352067 54.991055 45-49
AAAAA 3487440 2.1305842 14.901619 90-99
GTGGG 6679880 2.121276 29.765995 100-109
GTAGT 4289975 2.107136 24.872606 120-129
GCTAT 4076055 2.0838225 33.603172 50-59
TCTAT 3156120 2.0809171 20.381197 110-119
CCTCT 4489645 2.0733366 48.302 35-39
GCAGA 5621015 2.0720642 24.007862 140-149
TGCAG 5221330 2.0697665 42.879097 30-34
GCTGT 4853110 2.0687692 19.292257 230-239
CTCAC 4797945 2.0604465 56.184036 20-24
AGAGT 4430630 2.0237253 30.57966 150-159
TGCAC 4902270 2.0226483 27.241076 25-29
AACTA 3516555 2.0050042 36.64397 130-139
AACAG 4533980 2.004458 32.85933 210-219
TGTGA 4080045 2.0040233 19.318022 210-219
AGCAC 5175975 1.9859283 20.560017 130-139
TGAGC 4971145 1.9705919 17.911594 200-209
GGCTC 5720800 1.9681181 20.542847 40-44
CAGAG 5335470 1.9668044 31.897045 170-179
TCTCT 3429685 1.9624999 33.42664 35-39
ATGGA 4264720 1.9479446 12.087393 100-109
TGGTA 3962580 1.9463271 30.886168 120-129
ATATC 3172860 1.9453621 13.651925 160-169
TCCGC 5412370 1.9380492 41.700756 70-79
CCGTG 5616340 1.9321806 20.287117 230-239
CATAT 3144040 1.9276918 14.465914 160-169
GGTGG 6058780 1.9240382 18.589926 40-44
TTCAG 3758765 1.9216131 47.084057 45-49
GGTCT 4499990 1.9182423 35.600937 15-19
CAGAC 4996810 1.9171859 26.122911 140-149
CTGTA 3716525 1.9000183 24.135893 190-199
GGATC 4777230 1.8937228 18.34868 70-79
ACCCT 4359450 1.8721377 44.585114 10-14
GCCAG 5851775 1.8721052 35.138893 70-79
TCCAT 3515055 1.8704056 27.307491 45-49
TGGGG 5885375 1.8689713 122.31225 4
ACCAG 4866470 1.8671769 14.085761 190-199
GCACA 4858630 1.8641688 18.154087 130-139
CCTGC 5201805 1.8626504 49.835705 20-24
GTCAC 4504405 1.8584915 38.05041 160-169
CCAGA 4829680 1.8530612 33.40275 170-179
CTATT 2807025 1.850749 19.635963 110-119
ACTAT 3015040 1.8485986 17.00244 130-139
CAGTT 3607295 1.8441762 25.10551 190-199
TGTCT 3346675 1.839865 50.679474 30-34
TGGGA 4820050 1.8357301 16.587238 100-109
AGAGA 4314885 1.8327514 27.653967 170-179
AGGAC 4968010 1.8313483 20.50263 220-229
GATGG 4807540 1.8309656 20.88681 100-109
GCTGA 4601590 1.8240979 27.160034 200-209
TCAGC 4387725 1.8103501 11.85333 45-49
CACAG 4689975 1.7994589 15.76859 140-149
TTCCA 3368780 1.792571 22.439764 180-189
GAGGA 5030355 1.7815752 26.767933 220-229
GCCGA 5520600 1.7661554 19.13347 220-229
TAGTG 3595255 1.7659057 26.128397 120-129
ACCTG 4272240 1.7627016 31.17613 25-29
ATACT 2873700 1.7619395 26.343142 130-139
TACAA 3071210 1.7510855 17.980223 130-139
AGTCA 3681140 1.7500535 29.088888 160-169
CAAGG 4739315 1.7470448 16.988705 80-89
AGTTC 3410545 1.7435905 22.265524 190-199
TAGTA 2950060 1.7377907 16.804613 120-129
CTGAA 3616715 1.7194252 23.452768 200-209
TTCTG 3122995 1.716895 42.665207 30-34
TGACC 4152390 1.7132522 14.592438 210-219
CCTGG 4960045 1.7063965 34.796074 2
GCAAA 3857850 1.7055427 31.428385 200-209
CATGA 3559340 1.6921486 22.919693 60-69
GGAGC 5495840 1.6892496 13.106713 200-209
CTCAG 4064460 1.6769727 76.479164 6
AGCTA 3516490 1.6717772 25.521536 50-59
GTGCA 4210050 1.6688892 49.849823 25-29
GCCGT 4825985 1.660276 29.616547 230-239
ATGCA 3490085 1.659224 16.211075 60-69
GGATG 4346545 1.6553943 22.285357 100-109
ATTGG 3369735 1.6551356 20.83544 100-109
AACAC 3579815 1.647255 22.156912 190-199
AGGGG 5443165 1.6074158 32.669167 90-99
TCTCA 3018300 1.6060759 25.946898 100-109
CAGCT 3866975 1.5954915 12.977665 50-59
TCTGC 3581705 1.5891492 32.954884 200-209
GGGGC 6181765 1.5843217 28.597773 90-99
CTCCC 4246395 1.5826346 14.115177 190-199
GGGCC 5904615 1.5750902 27.74824 2
GTTCT 2854690 1.5693917 23.684174 190-199
TCTGA 3055075 1.5618619 21.099947 220-229
CGATT 3051525 1.5600469 34.51487 160-169
TTCTC 2716660 1.5544999 22.453089 190-199
CGTCC 4320480 1.5470675 19.227617 180-189
GCACT 3748980 1.5468075 26.17634 25-29
AGTTA 2585440 1.523004 16.080736 50-59
ACAAT 2664615 1.5192606 22.49099 180-189
GGCTT 3556570 1.5160841 21.301733 25-29
CGGCC 5428505 1.5072211 31.70965 230-239
CCATG 3650180 1.5060432 15.524926 160-169
GAGCA 4077445 1.5030609 7.588957 200-209
CTGGT 3505055 1.4941244 32.2497 35-39
AGTGA 3270190 1.493685 83.97604 9
CATCA 3008670 1.4887662 31.74294 45-49
GTCCG 4292255 1.4766577 33.92782 70-79
GTTAC 2882420 1.4735944 16.159172 50-59
TTAGT 2307195 1.4615129 25.115023 110-119
CTTTA 2213145 1.4591876 41.669903 45-49
GTGAC 3617480 1.433991 15.373038 210-219
TACGC 3471485 1.4323146 24.416182 140-149
CGTGT 3353590 1.4295584 24.13657 230-239
AAGAG 3364785 1.4291956 19.06007 150-159
ACTCC 3319630 1.4255936 16.896013 140-149
CTCAA 2861365 1.415876 18.779493 150-159
GTCCA 3388140 1.397927 28.867363 180-189
GGCAG 4535040 1.3939297 13.42048 150-159
CAACC 3480770 1.3900476 15.474347 140-149
GGGGT 4349520 1.3812422 116.56362 7
AACCC 3455900 1.380116 20.478113 140-149
TAGCA 2889915 1.3738967 11.524827 130-139
CAACT 2756930 1.3641989 17.674603 130-139
CTACG 3301265 1.362083 16.141857 130-139
AAGCT 2857525 1.3584982 16.881104 200-209
AGCAG 3683295 1.3577662 12.701415 50-59
GGCCT 3921825 1.3492193 67.81573 3
GAGAG 3804700 1.3474913 25.339252 220-229
ATCTA 2191410 1.3436099 20.895222 110-119
CACGT 3223410 1.3299603 16.744558 180-189
ATACA 2321135 1.3234217 21.49166 35-39
CGACA 3447390 1.3227015 16.013756 70-79
TGTCC 2979945 1.3221571 55.313766 15-19
GAACT 2779325 1.3213209 14.274658 190-199
TCAAG 2772020 1.3178481 23.439844 150-159
ACAGT 2770820 1.3172776 12.418782 120-129
GTGCG 3976535 1.3143657 17.438826 240-247
CCATA 2650885 1.3117251 14.825244 160-169
TTATT 1599215 1.3064889 20.470219 240-247
GTGGC 3947375 1.3047274 14.740177 40-44
TTACA 2124280 1.3024507 9.818818 120-129
TGGAA 2846745 1.3002733 18.963154 120-129
TGTGT 2459465 1.2990628 15.110562 240-247
ACAGA 2921225 1.291464 19.9172 140-149
AGAGC 3500920 1.2905376 24.169743 220-229
AACCA 2803210 1.2898996 17.430447 190-199
TCACT 2423870 1.2897722 13.320819 190-199
CCTTT 2246825 1.2856555 47.698307 45-49
ACATG 2701925 1.2845242 14.181681 190-199
CAATT 2095025 1.2845137 23.540976 180-189
AGACC 3329170 1.2773426 102.06506 9
CCAGT 3095095 1.2770183 19.109524 190-199
TCCGT 2875775 1.2759387 13.61474 150-159
ATCAT 2077870 1.2739956 15.68851 110-119
TGCGA 3211290 1.2729746 14.505798 240-247
CCTAC 2956975 1.2698538 17.105341 190-199
ATTAT 1652140 1.2551452 9.441334 50-59
GATTG 2546795 1.2509265 24.132627 100-109
ACACA 2715675 1.2496201 7.6859393 130-139
AATTC 2037670 1.2493479 23.495323 180-189
ACATA 2187865 1.2474363 20.51676 130-139
ATCCG 3021915 1.2468247 24.097698 70-79
ATATT 1636555 1.2433052 25.46673 240-247
ACGTC 3012730 1.2430351 18.491192 180-189
TCGGA 3116935 1.2355717 95.7615 5
AGTCG 3112090 1.2336512 16.304167 150-159
CTTCT 2143080 1.2262917 23.217075 30-34
CGGCT 3556130 1.2234097 21.10747 230-239
GATCT 2368630 1.2109269 9.441162 220-229
GTCGA 3017545 1.196173 12.7778015 150-159
TGGAC 3008255 1.1924905 15.734342 80-89
TACCA 2407730 1.1914059 10.857671 45-49
AGATC 2487285 1.182482 12.379756 210-219
CTTCG 2663885 1.1819263 108.22386 3
TGAGT 2406260 1.1818991 20.6975 90-99
GATCC 2862645 1.1811109 23.929428 70-79
CGCTG 3383130 1.1638929 12.311802 190-199
ATTAG 1960235 1.1547148 24.798119 110-119
AAGGC 3130660 1.1540495 28.306688 25-29
GGGTG 3620705 1.1497983 14.509989 60-69
TCGAG 2894530 1.147409 12.410225 150-159
ACGCA 2978065 1.1426299 20.746878 140-149
GAAGT 2498715 1.1413078 13.413223 140-149
GACCC 3422210 1.139547 43.750557 10-14
AGCTC 2757935 1.1379081 12.513006 210-219
GGACT 2857890 1.1328849 13.210905 90-99
TTGGG 2763385 1.1317497 15.557775 100-109
AGGCC 3536120 1.1312788 14.183023 2
GAACC 2945210 1.130024 10.61642 180-189
TTCGG 2633045 1.1224067 104.05545 4
ATTCC 2099190 1.1170058 21.9266 180-189
GCCCC 3840925 1.109981 12.419822 80-89
GTATA 1882745 1.1090679 10.175552 240-247
ACAAG 2493645 1.1024323 17.141142 80-89
AGGTC 2776590 1.100657 17.72516 10-14
GCACC 3288625 1.0950651 11.27695 130-139
CGCAG 3418330 1.0935953 16.344313 140-149
GCTTC 2462770 1.0926944 28.666246 2
GCAAG 2962975 1.0922359 16.772404 25-29
CCCCT 2919910 1.0882527 9.862468 140-149
TAGAC 2286000 1.086789 17.57258 170-179
GAAGC 2932405 1.080967 13.182541 200-209
CACAT 2172480 1.0749983 13.57651 130-139
TTGAG 2188295 1.0748397 20.470074 90-99
CTTGA 2094920 1.0709969 21.948347 90-99
CCGAT 2593370 1.0700095 23.028616 160-169
GTAGA 2335390 1.0667078 17.314924 170-179
GGAGA 3001535 1.0630385 85.14234 7
ATGGG 2788695 1.0620826 19.775972 100-109
TACAG 2230870 1.0605797 9.294771 120-129
CAGCA 2754710 1.0569326 12.645435 45-49
TGGCT 2467690 1.0519196 32.033752 40-44
ATCAA 1839140 1.0486068 12.400991 110-119
ACACC 2620375 1.0464483 13.062432 40-44
CTCCG 2899630 1.0382929 9.808599 140-149
CTATA 1692515 1.0377245 13.053624 50-59
GGGAG 3503835 1.0347142 14.572346 5
GCTCT 2331855 1.0346094 17.602346 210-219
ATGAG 2262605 1.0334628 19.769373 60-69
ATGGT 2101460 1.0321883 9.636077 35-39
ACCGC 3076105 1.024299 11.180808 220-229
GAGCC 3189340 1.0203366 17.79569 220-229
TAGTT 1602255 1.0149624 14.966391 50-59
GACAG 2747375 1.0127597 15.417414 70-79
CGTGA 2549675 1.0107064 20.231398 150-159
GTAGC 2526450 1.0015 11.116709 50-59
CGAGG 3255765 1.0007204 14.551935 220-229
AAACT 1752370 0.9991338 16.87861 130-139
ACTCA 2013475 0.9963185 12.180379 190-199
TTTCA 1509135 0.99501437 7.29998 100-109
TGAGG 2600990 0.9905946 11.809465 220-229
AGCCG 3096215 0.99054396 18.775871 220-229
CGAGT 2491605 0.9876871 15.129864 160-169
ACCAA 2133525 0.9817433 16.997257 130-139
TTATA 1287865 0.9784023 12.985949 110-119
CCGTC 2714705 0.9720753 10.4083605 140-149
ATGAC 2032320 0.9661869 10.669969 160-169
AACTC 1944505 0.9621904 15.923053 190-199
AAGGT 2104275 0.9611443 20.496897 15-19
ATGAT 1628025 0.9590201 8.765947 120-129
GCCAA 2491055 0.95577294 14.553955 180-189
CCAAC 2388840 0.9539847 14.139437 130-139
TTTAG 1504915 0.9533016 29.567064 45-49
GGGAC 3100635 0.9530384 10.173812 90-99
GATAC 1995970 0.9489056 23.477503 35-39
GCAGT 2392675 0.9484707 6.4378133 50-59
CCGAG 2964245 0.94832397 17.680637 220-229
CGGAC 2956450 0.94583017 10.962317 220-229
GGTAT 1924710 0.9453729 9.087673 110-119
AGCCC 2817945 0.9383354 11.572932 80-89
CGTAT 1834080 0.93764615 7.5315375 240-247
TTTAT 1143645 0.9343081 10.799809 240-247
GCCAT 2263070 0.93372965 7.589568 60-69
CATAC 1883975 0.93223864 13.873422 130-139
CAGAA 2100560 0.9286506 17.859621 140-149
ACAGG 2511875 0.9259478 14.953556 70-79
TACAC 1859730 0.9202416 21.51348 35-39
CCCAG 2760655 0.9192586 14.921377 80-89
TCATA 1492735 0.9152342 8.869387 110-119
AATCA 1604605 0.9148839 13.905059 110-119
TATGG 1862395 0.9147652 12.369356 35-39
AACTG 1923810 0.9146 11.480813 60-69
TAACA 1599600 0.9120302 8.672583 120-129
CCCCA 2629165 0.9112265 17.060007 80-89
TGGTT 1723095 0.9101202 9.425214 50-59
GAGAT 1985090 0.90670556 11.96819 210-219
AAGTT 1526330 0.89911467 17.340101 140-149
TCCTT 1563745 0.89479053 10.709891 1
GACTG 2253965 0.8934852 12.871081 90-99
CGGAG 2887045 0.8873874 74.13534 6
GTTAT 1379955 0.8741445 10.586747 110-119
AGAAG 2048995 0.8703125 15.636944 140-149
CATTA 1414140 0.8670456 12.157653 110-119
ACGCT 2101435 0.86704 15.057174 190-199
TATTT 1060070 0.86603093 10.485792 240-247
TCTTC 1501615 0.85923904 48.913994 2
ATAGT 1456460 0.85795635 15.605764 120-129
AGTCT 1655810 0.84650826 15.891524 8
TTACC 1590645 0.84640247 9.969746 45-49
GATGA 1852030 0.84592927 6.5104604 60-69
GGTAG 2220465 0.8456705 11.9174795 120-129
GTCTG 1980420 0.8442075 16.122782 3
GCCTA 2044955 0.84373665 16.475729 190-199
GTTTC 1534315 0.8435036 5.09304 100-109
GATCA 1765535 0.83935434 9.275617 110-119
CATGG 2100210 0.8325358 8.651696 200-209
CTATC 1561845 0.83107764 8.252385 110-119
ATCCA 1679435 0.83102703 9.69601 180-189
GTCTA 1620735 0.82857674 10.035816 30-34
GGTTC 1936575 0.8255174 11.256059 160-169
ACAAA 1545380 0.8193725 12.598312 130-139
GCTTG 1918565 0.81784016 16.920921 90-99
ATATG 1376800 0.811031 5.6249576 50-59
GGTGC 2451055 0.8101482 11.54324 60-69
CCTCC 2170235 0.8088481 10.802254 140-149
TCATC 1517265 0.80735606 10.450384 110-119
ACATC 1629100 0.80612 5.8087406 110-119
ATATA 1136600 0.80297697 10.579246 130-139
TGGCA 2022055 0.8015548 5.6033597 100-109
TTATG 1250000 0.79182345 11.158303 50-59
TATAC 1272205 0.7800216 8.922304 130-139
TGATG 1569550 0.77092654 11.730126 120-129
CCAGC 2276755 0.75812685 6.3708124 80-89
GTTCA 1474280 0.7537038 13.647274 160-169
CGCCG 2712010 0.752988 11.149651 220-229
CCCCC 2489415 0.7487898 13.557876 80-89
CTGAC 1811095 0.74724734 9.404576 220-229
GGTTA 1515060 0.7441622 8.356978 50-59
GCGGA 2404745 0.73914343 9.22449 220-229
GGGAT 1938720 0.73836714 13.216435 100-109
ATCTT 1118570 0.7375041 13.742471 3
GACCG 2282680 0.73027706 9.520909 210-219
TCAAC 1472135 0.72844976 7.473374 110-119
CATCC 1687070 0.7245013 6.625753 110-119
TTGGA 1474855 0.7244145 6.538723 60-69
ATAAA 1096440 0.7203252 7.579875 130-139
GATTA 1214220 0.7152601 5.5396066 50-59
AACGC 1860370 0.71379054 14.285374 180-189
TTTAC 1082465 0.7136991 13.533402 45-49
ACGCC 2140100 0.7126227 12.879316 180-189
TCCGG 2059485 0.7085215 11.827132 70-79
TATTG 1115570 0.7066676 6.4259777 240-247
ATGCC 1707230 0.70439327 8.0970955 50-59
GGGCA 2267495 0.6969571 13.434105 150-159
CACAA 1514295 0.6968041 8.3082695 130-139
TGCCA 1688025 0.69646937 7.290446 60-69
GACCA 1814260 0.69609886 6.0766253 170-179
GGATA 1519015 0.6938221 15.398092 35-39
CAACG 1802565 0.6916117 14.549359 180-189
AATAC 1203840 0.6863831 11.070401 130-139
TTTCT 967055 0.6856527 9.061276 4
AAATA 1043305 0.6854172 12.183892 130-139
TATAG 1160585 0.68366534 6.319144 120-129
CAAAC 1482445 0.6821483 13.57336 130-139
GAAAT 1240645 0.6796142 7.2136416 110-119
GCCGC 2426175 0.67362607 12.248968 220-229
CCCGT 1849400 0.6622288 10.155791 140-149
CAGTC 1598630 0.65958554 5.2699695 210-219
ATTAA 931565 0.65812534 6.27912 110-119
AGGCA 1773255 0.6536717 6.415723 80-89
CACGC 1953420 0.65046096 12.210206 190-199
AATAA 989050 0.6497734 7.1239357 130-139
GCGAC 2006680 0.6419789 7.148312 70-79
GACGA 1724070 0.6355407 9.642285 220-229
AGATG 1390955 0.6353297 5.598877 120-129
ACTTC 1193555 0.63510585 30.064518 4
CCGTA 1531445 0.6318654 6.372444 230-239
TCCAC 1460545 0.62722164 10.282408 180-189
TACTT 950780 0.6268756 11.465678 1
TCAAT 1016640 0.62332815 8.37997 110-119
CCGGC 2226630 0.61822253 9.6011305 70-79
AAGCA 1393265 0.6159579 5.9821863 120-129
CACCG 1844985 0.6143536 8.9642515 45-49
CCCTA 1406420 0.603978 11.745625 110-119
CACGA 1567565 0.60144645 8.781731 180-189
GGCCC 2158715 0.59936595 10.294362 70-79
GAAGA 1407650 0.5979006 6.320926 15-19
GGTGA 1568480 0.5973602 6.4716835 120-129
AGTAA 1089790 0.5969772 5.1643467 50-59
TAGCT 1159720 0.59288967 12.152754 50-59
AATGG 1292570 0.5903916 6.5886955 120-129
CTTTG 1065200 0.5856034 7.150145 45-49
TGATT 922635 0.58445126 5.9734735 50-59
ACCCG 1747085 0.58175427 8.831999 140-149
ACCTA 1167410 0.5776641 7.1238465 130-139
GGGGA 1955870 0.5775861 9.355721 4
ACGAC 1498570 0.5749743 9.008308 220-229
GTTCC 1277410 0.56676775 6.6546474 140-149
GTCCT 1265055 0.5612861 6.257897 45-49
TAATA 792020 0.55954057 7.7842913 130-139
TTCAA 905975 0.5554766 5.370962 200-209
TTAGC 1083985 0.55417126 9.539679 45-49
CGGTT 1296040 0.55247205 11.134093 160-169
ATTTC 835750 0.551033 6.1049747 240-247
CGGCA 1716620 0.5491826 10.972116 70-79
CTGCG 1590270 0.5470981 11.924883 25-29
TCAGG 1377055 0.54587287 5.3476543 150-159
AAGAT 995300 0.5452164 5.7381687 15-19
CCTAT 1008165 0.5364575 5.1294527 110-119
CTGTT 969085 0.5327633 5.7827854 240-247
GGAGG 1796995 0.5306689 5.33297 6
CCACG 1593600 0.53064597 7.7338877 180-189
GCTAC 1285725 0.5304827 7.951388 50-59
ACCCC 1526760 0.5291506 9.194345 140-149
AAATC 926720 0.52838004 7.772191 110-119
TATGT 815330 0.51647794 6.4433436 140-149
ACATT 837545 0.51352036 6.1830473 110-119
GGGTA 1302240 0.49596187 5.2982383 100-109
GGGTT 1210770 0.4958732 5.868843 100-109
CGCGG 1841445 0.4912161 6.6111455 220-229
GTAAC 1026835 0.48816845 6.820479 120-129
TGACG 1226045 0.48601156 8.534697 220-229
AGTAT 823245 0.48494858 5.16268 110-119
TATCC 897775 0.47771752 5.494866 110-119
AACAT 829720 0.47307435 5.676339 110-119
TGGCC 1361390 0.4683569 14.704011 2
CCGGT 1334585 0.45913517 8.656143 160-169
CAATC 925015 0.45772088 6.3014946 110-119
ATGCT 895290 0.45770377 7.07053 50-59
CCGCG 1637550 0.4546648 6.833345 220-229
CATAA 796520 0.45414498 5.7773046 110-119
GGTTT 858935 0.4536802 5.365797 100-109
TAAAT 641150 0.45295504 7.5583363 130-139
TGCTA 876115 0.44790083 6.349938 50-59
ATTCT 670405 0.4420165 7.4878745 2
TTGGT 830485 0.43865323 5.2628646 120-129
TGCGC 1271195 0.43732718 8.904049 25-29
GGTCA 1060340 0.42032516 5.3689256 160-169
GCCTT 946080 0.41976163 8.922041 1
GCGCT 1212040 0.41697615 8.104745 25-29
GCATG 1042510 0.4132572 8.08461 60-69
CACTT 769440 0.40942883 6.8932843 3
ACGAG 1103715 0.4068604 7.709973 180-189
CTAAC 812780 0.40218416 5.3338904 110-119
AGCTT 773920 0.39565513 7.5451508 1
ATGGC 993040 0.39364704 7.1804457 1
CCGGG 1470865 0.39236176 5.274267 80-89
CCTAA 785405 0.38863832 7.4384828 110-119
CATGC 931300 0.384249 6.830168 60-69
GGTAA 837435 0.38250506 7.168135 120-129
TTCTT 529645 0.37552413 20.731045 1
GAGGC 1220365 0.37510204 5.8096843 35-39
TTTGA 589530 0.37344295 5.874507 45-49
TGGGC 1051310 0.34748992 9.450954 1
CTCGG 1007045 0.34645215 5.268253 6
CGAGC 1064005 0.34039745 6.5556684 180-189
TGCTG 795825 0.33924192 13.452504 1
CTCTT 553645 0.3168012 9.611404 1
AACCT 635835 0.31462732 5.3128123 2
GCTTT 567420 0.31194434 5.126552 45-49
GCGAG 996475 0.30628526 5.3914914 240-247
TCACA 617475 0.3055423 5.4902673 5
CGCTT 678255 0.30093166 5.730978 1
CGGGA 973440 0.29920503 5.032188 80-89
AACTT 487555 0.29893255 10.819347 3
GACTT 571575 0.29220927 6.83276 1
ATTGT 450655 0.28547138 5.2883797 240-247
TTCCT 493595 0.28244 6.49654 4
TCGGT 640370 0.27297503 6.2421145 7
GAGGT 712675 0.27142435 6.738075 7
GTCTT 482800 0.26542372 12.0038395 1
TAACT 431885 0.26479983 7.0744996 2
CCCTT 571300 0.2638287 5.5911274 3
TGTAA 446925 0.26326993 7.9040575 25-29
TTGGC 607755 0.259072 7.118042 1
AATCT 414845 0.25435215 7.213096 2
CGGGG 970095 0.24862519 9.233135 3
CCTCG 668350 0.2393212 5.836093 5
TGCTT 407195 0.22385916 11.944754 1
TGCCT 439460 0.19498187 5.085026 4
TCGCA 439555 0.18135786 6.81188 7
TAGGC 382370 0.15157378 6.585462 1
TTGCT 228210 0.12546054 5.7404675 4
Produced by FastQC (version 0.10.1)