FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_2_SL248348.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_2_SL248348.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13388758
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 59467 0.44415620926153115 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 50797 0.37940038949094457 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 50490 0.3771074210169457 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 45927 0.3430265899196923 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 42864 0.320149187848492 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 42719 0.3190661897093069 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 41681 0.3113134168232781 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 40778 0.3045689525495942 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 39542 0.2953373270321265 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 39195 0.2927456004507662 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 38284 0.28594138455561 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 37704 0.2816093919988695 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 37407 0.279391113051711 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 37383 0.27921185818729416 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 36646 0.2737072400591601 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 36116 0.26974869513662136 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 35869 0.2679038638236646 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 35380 0.2642515459611713 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 35085 0.26204820491938086 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 34663 0.25889630688671794 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 34379 0.25677512432445193 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 34181 0.25529627169301294 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 34063 0.2544149352762967 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 33975 0.2537576674401016 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 32526 0.24293515500093438 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 30846 0.23038731449175495 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30179 0.22540552305150338 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30124 0.22499473065388143 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29863 0.22304533400334817 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29188 0.2180037909416243 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 28939 0.21614402172329952 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 28551 0.21324606808189378 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27929 0.20860037951242377 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27242 0.2034692090184915 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27116 0.20252812098030304 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26699 0.19941356771106025 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26551 0.19830816271382304 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26471 0.1977106464991002 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 25670 0.19172801539918788 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25269 0.1887329653728897 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 25038 0.18700763730287753 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 25022 0.186888134059933 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24370 0.1820183769099419 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24299 0.18148808126937538 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23494 0.17547557435872693 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23470 0.17529631949431007 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23381 0.17463158270543094 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22826 0.1704863139657913 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22189 0.16572859110606078 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 21811 0.1629053269914954 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 21686 0.16197170790599097 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21434 0.16008953182961408 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 21400 0.15983558743835688 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20787 0.15525711944304318 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20769 0.15512267829473056 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20553 0.15350938451497892 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 19822 0.14804958010294905 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 19423 0.14506946798201895 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 19008 0.14196985261814427 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 17982 0.13430670716432397 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 17055 0.1273829880262232 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 16446 0.12283439584164564 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 16214 0.12110159881894945 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 16125 0.12043686203007031 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15393 0.11496958866535642 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 14666 0.10953966006406271 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14519 0.1084417240195095 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13822 0.10323586399873685 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 27527485 7.751515 69.18525 35-39
TATTA 13803340 7.5669975 132.40279 240-247
TGGAT 20675510 7.2133145 82.537575 35-39
TCACC 21400050 6.570097 76.76293 160-169
TCTCC 19141590 6.259338 89.459946 20-24
CTGTG 20138090 6.0399284 91.00053 25-29
TCCCT 17973865 5.8774896 73.73535 15-19
AGTGG 21427135 5.778508 40.682636 100-109
CTGAG 20461370 5.7617545 52.735165 15-19
TGTAT 12707370 5.7353706 61.487686 240-247
TTACT 11475845 5.408277 114.71636 240-247
ATTAC 11542685 5.1072564 109.37148 240-247
CCTGA 17125175 5.0352783 54.439728 15-19
TGGGT 17440870 5.009716 47.319923 100-109
TTCAC 12923930 4.9159813 79.37712 40-44
CTCTG 15359670 4.810208 76.37804 35-39
CTCCA 15657170 4.8069572 43.94139 80-89
ACACG 17194235 4.7465487 51.467144 230-239
TGAGA 14255515 4.669476 100.09779 15-19
GACAC 16865490 4.655797 48.456806 230-239
ATTCA 10371245 4.588933 119.71989 40-44
AAGGG 17502470 4.4315686 40.554375 90-99
TACTG 11955650 4.3553224 80.586555 240-247
TCTGG 14478635 4.3425126 138.66652 35-39
AGCCT 14657880 4.309825 54.747902 30-34
GAAGG 16854930 4.267614 33.063015 150-159
CAGCC 17866220 4.239968 59.37106 30-34
CCAGG 18612830 4.2303257 55.389797 80-89
GAGAC 15946850 4.216009 86.37399 8
CCCTG 16630250 4.203607 87.80747 10-14
ACCAT 11739455 4.192471 69.51088 160-169
TCCAG 14159420 4.1632643 43.178608 80-89
TGGAG 15406780 4.1549273 39.852757 90-99
GGGTC 17625660 4.0863147 133.06882 8
ACTAC 11418465 4.0778375 36.41173 130-139
TCAGT 11160565 4.065681 93.02172 7
TACTA 9130755 4.0400567 31.528217 130-139
GTATT 8888685 4.0118375 71.0581 240-247
CACCA 13914975 4.0109377 59.056458 160-169
GACTC 13391450 3.93746 54.79447 20-24
AGACT 11491910 3.9304833 73.93911 15-19
GATTC 10700600 3.8981209 62.286907 40-44
CTGGG 16701340 3.8720214 140.14056 3
ACTGT 10559435 3.846696 69.109726 240-247
CACCT 12485325 3.833159 45.248497 40-44
TGAAG 11570615 3.7900217 42.34224 150-159
CAAGA 11662885 3.7451248 46.320145 180-189
GGACA 13843610 3.6599572 29.20398 220-229
GCTGG 15696645 3.6390939 35.022472 2
ACTCT 9455395 3.596626 72.24734 20-24
ACTGG 12635310 3.558 44.662586 60-69
CCTGT 11336135 3.550152 63.614655 25-29
TATCT 7348005 3.46293 52.135914 200-209
AGACA 10542220 3.3852627 39.56514 170-179
TCCTG 10778275 3.3754463 64.734955 20-24
GGATT 9671160 3.3740947 59.3767 40-44
GAGTG 12495905 3.369918 39.456657 100-109
GGTCC 13785480 3.3371582 148.73318 9
CTCCT 10147775 3.3183427 96.29225 20-24
AGCTG 11559625 3.255096 37.67067 60-69
CATCT 8515070 3.238947 34.09856 170-179
CTGCA 10997335 3.2335234 45.099503 25-29
CCATC 10241920 3.1444042 35.596127 45-49
GTCTC 10013695 3.1360023 33.57882 30-34
GTCCC 12371985 3.127251 78.78685 10-14
AAGAA 8223290 3.0716455 39.597973 180-189
AGTAG 9306595 3.0484288 30.601622 50-59
GGAGT 11264190 3.037747 38.256935 90-99
GGCTG 13025550 3.0198295 36.066013 90-99
CCTCA 9702255 2.97872 79.03523 5
AGGGA 11538620 2.9215412 42.335262 80-89
GAACA 8960700 2.8774133 37.47482 210-219
TGTGC 9589320 2.8760822 60.551506 25-29
GAGCT 10201860 2.8727605 31.02186 60-69
GTGAA 8626450 2.825643 41.16103 150-159
GACAA 8792840 2.823511 26.19108 180-189
ATCTG 7740360 2.8197348 38.784924 200-209
GGAAG 11123645 2.8164709 31.755283 80-89
ATGAA 7064375 2.8105583 29.3324 210-219
ATCTC 7360480 2.799766 50.51919 170-179
CAGGG 12746465 2.774487 37.92309 80-89
ACCTT 7253095 2.7589192 72.85969 40-44
GTGTA 7766390 2.7095544 36.479725 230-239
CTATG 7428000 2.7059453 28.45188 50-59
AGAAC 8277640 2.6580722 32.22522 190-199
CGCCA 11136585 2.6429071 41.60282 70-79
GGGAA 10437890 2.64284 35.211113 80-89
CACGG 11512025 2.6164541 45.757965 230-239
TGCAA 7599275 2.5991173 37.914265 200-209
GAGTC 9019205 2.5397344 27.590048 150-159
AGGGC 11645770 2.534902 33.7273 150-159
CCAAG 9180810 2.5344055 40.520508 180-189
AGGCT 8982135 2.529296 21.246725 80-89
ACGGC 10997965 2.4996185 50.442932 230-239
CCCTC 9394790 2.4795842 27.044622 20-24
TATAT 4512050 2.4735084 26.06878 240-247
CAGTG 8738280 2.4606283 71.90472 8
TGAAC 7189315 2.4589021 40.013058 210-219
ATCAG 7180020 2.4557228 27.647905 45-49
CTACA 6872325 2.45429 19.143486 190-199
CAAAT 5864680 2.436305 38.285664 200-209
CACTG 8270985 2.4319003 19.901651 30-34
GCAGC 10682155 2.4278412 36.42044 30-34
CTCTC 7423505 2.427501 103.01461 20-24
TCTGT 6184465 2.3996212 24.530285 210-219
CCGCC 11740125 2.395176 35.719414 70-79
ACAGC 8595435 2.37281 29.037884 210-219
CTTCA 6224495 2.3676622 51.98942 45-49
CTACT 6215685 2.364311 22.644363 50-59
GTGGA 8758925 2.3621223 26.227467 100-109
GGGCT 10176245 2.35925 43.786533 90-99
GTATC 6467110 2.3559031 24.354183 200-209
CTGTC 7487080 2.3447387 48.596848 30-34
AAATG 5893365 2.3446732 36.279705 200-209
AATGA 5849990 2.327416 27.508476 210-219
GGCCG 12391545 2.3187463 25.397629 230-239
TCCAA 6460990 2.3073914 44.28493 180-189
TATGC 6324995 2.304132 21.449202 50-59
CAGGC 10088610 2.2929404 34.009987 70-79
ACAAC 6816300 2.28548 21.625782 180-189
GTGGT 7947155 2.282741 32.73584 120-129
GCTCC 8969665 2.2672508 35.86433 80-89
GCCTC 8966625 2.266482 68.997955 4
GTAGT 6470900 2.2575812 27.71112 120-129
CAGTA 6566290 2.2458138 22.69359 45-49
TGGTG 7779925 2.234706 34.063286 35-39
GGGGG 13013715 2.2335317 100.16431 5
GCCTG 9196145 2.2261817 31.584616 210-219
TACAT 4969360 2.1987772 18.079092 190-199
GCTAT 5930965 2.160591 34.72789 50-59
GCTGT 7178070 2.1528869 20.244137 230-239
CCTCT 6581755 2.152247 51.55483 35-39
GTGGG 9692730 2.152108 31.433298 100-109
TGCAG 7633620 2.149565 46.03511 30-34
CCTTC 6512085 2.1294649 56.84826 45-49
AACAG 6618075 2.1251616 36.08392 210-219
GCAGA 7972800 2.1078393 26.08106 140-149
TATCA 4719715 2.0883179 17.541569 170-179
CAGAG 7802115 2.0627139 34.672855 170-179
TCTAT 4370575 2.059742 19.820833 110-119
GGCTC 8381465 2.028966 20.015587 40-44
TCCGC 8014610 2.0258427 44.07492 70-79
AACTA 4865475 2.0212154 36.263405 130-139
CTGTA 5540410 2.0183156 26.495636 190-199
TGAGC 7135815 2.0093873 17.483053 200-209
CTCAC 6539380 2.0076756 54.30738 20-24
AGCAC 7257985 2.0036006 21.836288 130-139
TGGTA 5723105 1.9966888 32.22165 120-129
TGCAC 6783280 1.9944736 26.80935 25-29
TGTGA 5680480 1.9818178 18.797018 210-219
CATAT 4470880 1.9782165 14.342869 160-169
CCGTG 8092925 1.9591168 19.851315 230-239
TTCAG 5376155 1.9584792 48.547874 45-49
CAGAC 7092420 1.9578956 28.445929 140-149
ATATC 4416970 1.954363 13.756278 170-179
ATGGA 5964040 1.9535555 12.201964 120-129
GGTCT 6497220 1.9486825 34.12297 15-19
AGAGA 6317260 1.9427663 30.35443 170-179
CCAGA 7034300 1.941851 36.453545 170-179
GCCAG 8531745 1.939096 36.961327 70-79
TAGTA 4541380 1.9244213 18.99805 120-129
TGGGG 8653635 1.9213942 130.19617 4
AGAGT 5851465 1.916681 30.286991 150-159
CTATT 4064915 1.915692 20.726439 110-119
AGGAC 7211275 1.9065082 21.752514 220-229
ATACT 4296890 1.9012316 29.29273 130-139
TCTCT 4678120 1.8953106 33.274494 35-39
GCCGA 8288915 1.8839054 20.95925 220-229
ACTAT 4254625 1.8825308 17.295095 130-139
GCACA 6809745 1.8798616 19.254593 130-139
GGTGG 8363430 1.8569592 18.265875 40-44
CAGTT 5092495 1.8551445 25.006926 190-199
TCCAT 4869000 1.8520614 27.23997 45-49
GAGGA 7307845 1.8503227 28.419598 220-229
ACCAG 6635310 1.8317083 14.3105545 190-199
TAGTG 5248510 1.8311112 27.449799 120-129
TTCCA 4812540 1.8305852 24.28165 180-189
GGATC 6495290 1.8290206 18.20346 70-79
ACCCT 5936290 1.8225192 42.72681 10-14
GTCAC 6187650 1.8193418 37.281853 160-169
GATGG 6717495 1.8115861 20.156858 100-109
GCAAA 5639575 1.8109509 34.468906 200-209
TGGGA 6698810 1.8065468 16.098316 100-109
CATGA 5242155 1.7929311 25.712418 60-69
CCTGC 7072050 1.7875929 47.981846 20-24
GCTGA 6339920 1.7852697 26.450825 200-209
TGTCT 4599815 1.7847646 49.77841 30-34
TCAGC 6021545 1.7705022 11.474205 45-49
GTGCA 6267285 1.7648162 53.711834 25-29
CACAG 6375180 1.7598982 15.504883 140-149
AGCTA 5101375 1.744781 27.064478 50-59
TACAA 4179560 1.7362726 17.753237 130-139
AACAC 5177625 1.7360384 24.322031 190-199
AGTCA 5057320 1.7297134 28.737532 160-169
AGTTC 4715140 1.7176781 22.070124 190-199
ACCTG 5815120 1.7098075 30.410624 25-29
CAAGG 6460675 1.7080655 16.880135 80-89
CTCAG 5759830 1.6935507 75.34785 6
TGACC 5738475 1.6872717 14.224783 210-219
TTCTG 4332070 1.6808776 41.034306 30-34
CTGAA 4911475 1.6798313 23.4915 200-209
AGGGG 8051025 1.6783236 34.64591 90-99
CCTGG 6894330 1.6689637 36.078785 2
TCTGC 5318235 1.6655185 35.10305 200-209
ATGCA 4866255 1.6643649 16.501753 60-69
ATTGG 4718890 1.6463364 20.372562 100-109
GGGGC 9150345 1.6398224 29.995186 90-99
TCTCA 4297610 1.6347171 28.492002 100-109
GGAGC 7510050 1.6346916 12.628892 200-209
CGATT 4484930 1.6338147 36.166393 160-169
GGATG 6057515 1.6336015 21.688408 100-109
GGGCC 8630175 1.6149064 27.371195 2
ACAAT 3856565 1.602094 24.687479 180-189
GCCGT 6572380 1.591027 29.476025 230-239
AGTTA 3720505 1.5765736 16.459146 50-59
CAGCT 5357570 1.5752747 13.153166 50-59
TTAGT 3490005 1.575186 27.523098 110-119
GTCCG 6436330 1.5580924 36.04675 70-79
CTCCC 5805290 1.5322009 13.828335 190-199
GCACT 5193650 1.5270777 25.776476 25-29
GTTCT 3918455 1.5203916 23.207441 190-199
CCATG 5163560 1.5182307 15.257904 160-169
TCTGA 4157940 1.5146955 20.683376 220-229
CGTCC 5979740 1.5114913 18.694996 180-189
CTTTA 3199240 1.5077212 43.837517 45-49
AGTGA 4600580 1.5069464 83.265915 9
CGGCC 7706500 1.5057518 31.777182 230-239
ACTCC 4882930 1.4991238 18.405079 140-149
TTCTC 3693780 1.4965116 21.88178 190-199
TAGCA 4334585 1.4825222 12.512442 130-139
TACGC 5026590 1.4779577 26.322792 140-149
GTTAC 4033185 1.4692485 15.762667 50-59
GAGCA 5530540 1.4621576 7.514964 200-209
GGCTT 4862365 1.4583476 20.911226 25-29
GGGGT 6518740 1.4473768 124.78476 7
CATCA 4044145 1.4442717 32.017956 45-49
GAGAG 5583805 1.4138014 27.508215 220-229
CTCAA 3954320 1.4121928 19.024874 150-159
CTACG 4771840 1.4030541 16.83084 130-139
GTGAC 4971995 1.400073 14.989525 210-219
GAACT 4091555 1.3994008 15.687879 190-199
CAACT 3916315 1.3986202 17.417442 130-139
CTGGT 4641230 1.3920237 31.382137 35-39
CAATT 3124005 1.3822689 25.708792 180-189
GGCAG 6250930 1.3606223 13.237563 150-159
AAGCT 3977100 1.3602548 16.809483 200-209
CAACC 4717950 1.359931 15.680636 140-149
GTCCA 4606180 1.3543454 28.135014 180-189
AGCAG 5109705 1.3508977 13.502677 50-59
AACCC 4666075 1.3449782 20.469246 140-149
CCATA 3765260 1.3446745 14.801117 160-169
AATTC 3037715 1.3440883 25.630114 180-189
ACATA 3225850 1.3400824 23.089052 130-139
CCTTT 3292830 1.3340691 50.66234 45-49
ATCTA 3009975 1.3318143 20.649368 110-119
TCCGT 4251835 1.3315527 14.44365 150-159
TGGAA 4043680 1.3245306 19.711468 120-129
TTATT 2268130 1.324344 21.160873 240-247
AGAGC 5002505 1.3225563 26.455908 220-229
CGTGT 4396865 1.3187324 23.529966 230-239
TGTGT 3537235 1.3144257 15.721919 240-247
ATATT 2392655 1.3116547 27.84771 240-247
CACGT 4459645 1.3112597 16.795568 180-189
TCAAG 3823660 1.3077748 23.406588 150-159
TCACT 3435720 1.306873 14.708154 190-199
CGACA 4709990 1.3002145 15.859852 70-79
ATACA 3122455 1.2971301 21.688122 35-39
GGCCT 5347940 1.2946173 65.9125 3
ACAGT 3770750 1.2896786 12.367867 120-129
ACATG 3763700 1.2872673 14.208245 190-199
TTACA 2905910 1.2857691 9.637115 120-129
GTGGC 5532975 1.2827591 14.36243 40-44
CCAGT 4330120 1.2731758 19.061523 190-199
TGTCC 4062220 1.2721707 53.48799 15-19
AACCA 3769580 1.2639261 17.934458 190-199
AAGAG 4099870 1.2608457 18.987032 150-159
ACAGA 3917830 1.2580731 19.772205 140-149
GTGCG 5421735 1.2569692 16.843338 240-247
CGGCT 5132950 1.242573 22.153103 230-239
ATTAG 2921615 1.2380419 27.233562 110-119
TGCGA 4387840 1.2355798 14.113903 240-247
ATTAT 2246775 1.2316834 9.203094 50-59
GATTG 3510030 1.2245867 23.650644 100-109
CCTAC 3987080 1.224086 16.785543 190-199
AGACC 4427210 1.222152 97.737915 9
ATCCG 4143910 1.2184252 23.968088 70-79
ACGTC 4129415 1.2141632 18.55021 180-189
ACGCA 4394410 1.2130972 22.647324 140-149
AGTCG 4280530 1.2053623 15.863952 150-159
ATTCC 3143380 1.1956732 23.72835 180-189
TACCA 3329095 1.1889083 10.555688 45-49
ACACA 3544255 1.1883755 7.770459 130-139
TGGAC 4183525 1.1780463 15.329905 80-89
CTTCT 2899655 1.1747768 22.180937 30-34
GTCGA 4151280 1.1689665 12.364466 150-159
GATCC 3952915 1.1622673 23.804085 70-79
CGCTG 4795210 1.1608136 13.349879 190-199
GAAGT 3481870 1.1405066 13.149746 140-149
CTTCG 3641215 1.140324 103.20585 3
GGACT 4046465 1.1394515 12.877903 90-99
TGAGT 3258170 1.1367172 20.177355 90-99
CCGAT 3855060 1.1334952 24.587036 160-169
CGCAG 4961505 1.1276515 17.823267 140-149
AGCTC 3834050 1.1273177 12.650919 210-219
GTATA 2652020 1.1238003 10.950692 240-247
TCGGA 3988315 1.1230769 92.09283 5
TCGAG 3983065 1.1215986 12.020522 150-159
ATCAT 2524645 1.1170719 15.28796 110-119
CACAT 3127370 1.1168668 15.390587 130-139
GGGTG 5013735 1.1132157 13.905806 60-69
TTGGG 3872025 1.1122006 15.114645 100-109
ATGAG 3390930 1.1107186 21.955667 60-69
GAACC 3997110 1.1034209 10.469954 180-189
AAGGC 4168725 1.1021225 27.994587 25-29
GCAAG 4113820 1.0876068 16.187002 25-29
GAGCC 4775895 1.085466 19.506092 220-229
CTCCG 4289355 1.0842148 10.673887 140-149
GACCC 4567500 1.083948 41.961002 10-14
TTCGG 3603055 1.0806484 99.036194 4
GTAGC 3824630 1.0769845 12.271106 50-59
TAGAC 3147960 1.0766706 17.412945 170-179
GATCT 2954720 1.0763748 9.00497 220-229
AGGCC 4723975 1.0736656 13.5421095 2
GAAGC 4056645 1.0724909 13.10661 200-209
CGTGA 3793090 1.0681032 21.780567 150-159
ACAAG 3326165 1.0680808 17.101526 80-89
GCCCC 5210480 1.0630224 12.168681 80-89
GCACC 4478170 1.0627484 11.329465 130-139
GCTTC 3392005 1.0622787 27.112156 2
CGAGG 4862225 1.0583467 15.887272 220-229
AGCCG 4634150 1.0532501 20.630533 220-229
AGGTC 3738840 1.0528268 17.54349 10-14
CTATA 2366585 1.0471355 13.710933 50-59
ATCAA 2504380 1.0403694 12.402671 110-119
CTTGA 2853770 1.0395995 21.397167 90-99
TTGAG 2971670 1.0367625 19.937521 90-99
ATGGG 3831835 1.0333759 19.15265 100-109
CCCCT 3912220 1.0325595 9.313504 140-149
GCCAA 3740000 1.0324445 15.868398 180-189
CAGCA 3731545 1.0301102 12.313677 45-49
AACTC 2868460 1.0244033 17.552074 190-199
TAGTT 2269065 1.0241245 15.06289 50-59
GGAGA 4035350 1.0217376 82.46635 7
TTTAG 2260855 1.020419 32.3549 45-49
ACACC 3536540 1.0193942 12.826372 40-44
GCTCT 3248615 1.0173731 17.349432 210-219
TGGCT 3385690 1.015455 31.266066 40-44
ACTCA 2838090 1.0135574 13.614844 190-199
ATGGT 2902535 1.0126424 9.548972 35-39
CCGAG 4447120 1.010742 19.45595 220-229
GGGAG 4845510 1.0100992 13.669616 5
TTTCA 2136155 1.0067161 8.098797 100-109
TACAG 2940265 1.0056345 8.856639 120-129
TTATA 1830770 1.003629 13.907787 110-119
CATAC 2796745 0.998792 15.596762 130-139
ACCGC 4168905 0.9893544 11.302636 220-229
GACAG 3739575 0.9886643 15.223503 70-79
AAACT 2362000 0.98122203 16.718723 130-139
TGAGG 3634490 0.9801558 11.666458 220-229
AGATC 2851190 0.9751689 12.333154 210-219
GTAGA 2946955 0.9652921 17.134726 170-179
CCGTC 3816905 0.96479416 10.232752 140-149
CGAGT 3422715 0.9638085 15.225432 160-169
ATGAC 2810255 0.9611682 10.769135 160-169
AACTG 2808950 0.960722 12.759818 60-69
TTTAT 1627780 0.9504486 11.38981 240-247
GCCAT 3215295 0.9453864 8.233265 60-69
ACCAA 2817305 0.9446318 17.043236 130-139
CCAAC 3265070 0.9411438 13.897572 130-139
ATGAT 2203105 0.93357134 8.932618 120-129
GGGAC 4283355 0.9323459 9.89589 90-99
GGTAT 2670710 0.93176293 9.443535 110-119
AAGGT 2838805 0.9298669 19.668062 15-19
CGGAC 4082055 0.9277699 10.688133 220-229
GCAGT 3288570 0.9260344 6.548895 50-59
TCATA 2090655 0.9250456 8.969155 110-119
GTTAT 2026210 0.91451377 11.150139 110-119
TATGG 2614645 0.91220295 12.288161 35-39
AGCCC 3834520 0.90999895 11.571498 80-89
TAACA 2189960 0.9097531 8.8456745 120-129
AATCA 2184440 0.90746 13.800075 110-119
GATAC 2649375 0.9061439 23.316563 35-39
TATTT 1550190 0.9051443 11.014788 240-247
ACAGG 3411665 0.9019719 14.7663145 70-79
CAGAA 2799530 0.8989705 17.662596 140-149
GGTAG 3322760 0.8960879 12.926956 120-129
CCCAG 3765810 0.8936929 14.694383 80-89
TACAC 2488535 0.88872194 21.798437 35-39
TGGTT 2377780 0.8835758 9.231159 50-59
ACGCT 3004805 0.88349634 16.474638 190-199
GACTG 3115445 0.8772839 12.524762 90-99
AAGTT 2064295 0.8747503 17.045952 140-149
CGTAT 2400850 0.8746055 8.013803 240-247
GAGAT 2651940 0.86865824 11.901176 210-219
CCCCA 3493095 0.8655831 16.703058 80-89
GATGA 2603280 0.85271937 7.0667486 60-69
CGGAG 3917190 0.85264367 71.13005 6
GTTTC 2191860 0.85045904 5.7014165 100-109
ATAGT 2000055 0.8475284 15.753888 120-129
GGTTC 2810580 0.8429649 12.116868 160-169
TCCTT 2074855 0.8406143 10.732023 1
TCTTC 2070705 0.838933 44.60138 2
AGAAG 2700865 0.8306052 15.433089 140-149
ATATA 1605245 0.82620406 11.911627 130-139
AGTCT 2267530 0.82603836 14.47118 8
GTCTG 2740305 0.8218876 14.814963 1
GTCTA 2246705 0.818452 9.950753 30-34
TTACC 2150475 0.8179938 9.395307 45-49
GCCTA 2767490 0.8137191 16.131533 190-199
TGGCA 2882560 0.8117053 5.7439775 100-109
ATCCA 2265660 0.80912745 9.426738 180-189
CATGG 2868550 0.80776024 8.221517 200-209
TATAC 1818895 0.80480087 9.958744 130-139
TTATG 1768385 0.7981465 11.868363 50-59
GTTCA 2189210 0.79750705 14.838843 160-169
GATCA 2324295 0.7949594 8.810858 110-119
AAAAA 1741725 0.79020315 5.3058224 190-199
CATTA 1774625 0.7852129 13.321425 110-119
ATATG 1852170 0.78486174 5.565106 50-59
TCATC 2049460 0.77956986 9.916909 110-119
ACATC 2180980 0.77888596 5.8516865 110-119
CTATC 2046390 0.77840215 7.7785378 110-119
ACAAA 1992820 0.77725226 12.850293 130-139
GCTTG 2574795 0.7722469 16.280268 90-99
GGTGC 3321650 0.7700879 11.205251 60-69
TGATG 2189015 0.76370823 12.09847 120-129
CCTCC 2892990 0.7635522 10.23363 140-149
AACGC 2748835 0.7588287 15.574563 180-189
ACGCC 3139130 0.7449707 13.870166 180-189
CCAGC 3125065 0.7416328 6.5477486 80-89
CAACG 2678265 0.7393475 15.866352 180-189
ATCTT 1561165 0.73573774 12.27769 3
GCGGA 3362980 0.7320104 9.09574 220-229
GGTTA 2095265 0.73100054 8.227056 50-59
ATGCC 2471275 0.7266238 8.728379 50-59
TGCCA 2471125 0.72657967 7.906637 60-69
TCAAC 2027000 0.72389555 7.368544 110-119
CTGAC 2461615 0.7237835 9.2813635 220-229
TGACT 1982995 0.7223851 5.0159097 2
ATAAA 1486580 0.71835786 7.8747134 130-139
GACCG 3150630 0.7160755 9.152749 210-219
TATTG 1584505 0.71515375 6.526575 240-247
TTGGA 2036405 0.71046525 6.3910017 60-69
CCCCC 3334850 0.71041065 13.288407 80-89
GGGAT 2629575 0.7091485 12.919286 100-109
TATAG 1665070 0.70557773 6.5238066 120-129
GAAAT 1766425 0.7027714 7.2806134 110-119
TTTAC 1485635 0.7001424 12.865302 45-49
AATAC 1673430 0.6951762 11.804796 130-139
GGGCA 3159165 0.68764657 13.2525625 150-159
CACGC 2889665 0.68576825 13.342529 190-199
TTTCT 1366060 0.68570524 9.506671 4
CATCC 2226050 0.6834266 6.412054 110-119
GACCA 2466045 0.6807631 5.971026 170-179
CGCCG 3482570 0.6804498 10.855394 220-229
TCCGG 2803650 0.6787012 11.364978 70-79
GATTA 1587715 0.67279834 5.4258213 50-59
CACAA 2003345 0.67171407 8.316598 130-139
GGATA 2040870 0.66849875 15.003994 35-39
AAATA 1373090 0.66351634 13.096099 130-139
GCCGC 3382350 0.66086805 12.03242 220-229
CAAAC 1963855 0.65847325 13.479832 130-139
CCCGT 2578875 0.6518589 10.031959 140-149
CAGTC 2186310 0.64283615 5.512281 210-219
TAGCT 1740935 0.63420504 13.322875 50-59
TACTT 1343780 0.63328964 11.151884 1
AGCAA 1966560 0.6314915 5.1814265 50-59
AGGCA 2377480 0.62855536 6.4370065 80-89
AGATG 1916930 0.6279015 6.01508 120-129
TTCAT 1330365 0.62696755 5.08796 100-109
GACGA 2361945 0.6244482 9.636403 220-229
GCGAC 2744250 0.62371343 6.9065256 70-79
AAGCA 1934850 0.6213089 5.94673 120-129
ACTTC 1632335 0.6209046 30.375204 4
TCAAT 1397855 0.6185046 8.376643 110-119
CACCG 2531905 0.60086554 9.153835 45-49
TTAGC 1647155 0.600042 10.578507 45-49
CCGGC 3059590 0.59780484 9.225941 70-79
AATAA 1230550 0.59463686 7.3520913 130-139
AATGG 1810475 0.5930315 6.633692 120-129
AGTAA 1485460 0.59098965 5.2883687 50-59
ACCTA 1640170 0.5857483 7.553155 130-139
CGGTT 1948070 0.58427596 12.024423 160-169
ACCCG 2453500 0.58225864 8.953919 140-149
TTCAA 1315160 0.5819148 6.213328 200-209
CCGTA 1976030 0.58100784 6.8547955 230-239
CCCTA 1891735 0.58078754 11.471764 110-119
TCCAC 1874345 0.5754486 9.785908 180-189
ACGAC 2078810 0.5738652 8.96969 220-229
CACGA 2062710 0.56942064 8.417815 180-189
GGCCC 2886045 0.5638964 9.77175 70-79
CTTTG 1441880 0.5594608 7.012612 45-49
ATTTC 1181035 0.55659205 6.4755874 240-247
TAATA 1070110 0.55077523 8.029961 130-139
GTTCC 1755195 0.5496767 6.5251527 140-149
TGATT 1213240 0.5475862 5.824408 50-59
GGGGA 2620990 0.5463738 8.441801 4
GGTGA 2013715 0.54306227 6.293535 120-129
ATTAA 1054240 0.5426071 6.7288694 110-119
TCAGG 1901180 0.53535676 5.189332 150-159
CGGCA 2352850 0.534756 10.639806 70-79
ACATT 1205585 0.5334315 6.8306694 110-119
CTGTT 1369085 0.53121585 5.8674374 240-247
CTGCG 2176650 0.52691853 11.843799 25-29
TATGT 1147050 0.5177119 7.038414 140-149
GGAGG 2455545 0.51188505 5.358772 6
AAATC 1228985 0.51054496 7.847312 110-119
AAGAT 1280270 0.50935495 5.671952 120-129
GCTAC 1731595 0.5091372 7.6944485 50-59
CCACG 2143060 0.50858575 7.3690495 180-189
GTCCT 1607255 0.5033462 6.3724933 45-49
GAAGA 1625515 0.49989963 5.8396077 15-19
ACCCC 1983970 0.4916244 8.869679 140-149
GGGTT 1710365 0.4912854 6.2453165 100-109
GTAAC 1423870 0.4869945 6.9417377 120-129
CGCGG 2595875 0.4857486 6.550464 220-229
CCGGT 1987265 0.48107266 9.355353 160-169
GGGTA 1769390 0.4771723 5.0257764 100-109
AGTAT 1124470 0.47649705 5.3385177 110-119
ATTTA 867995 0.4758353 5.0189567 110-119
AACAT 1142630 0.4746713 6.216956 110-119
TGACG 1678160 0.47255614 8.40179 220-229
TGGCC 1943810 0.4705531 14.179501 2
CATAA 1130510 0.4696364 6.3819876 110-119
GGTTT 1253735 0.4658841 5.91216 100-109
CCATT 1205265 0.45845652 5.206563 110-119
CAATC 1279205 0.45683813 6.2487288 110-119
CCGCG 2278275 0.44514588 6.797566 220-229
TAAAT 848850 0.43689486 7.859242 130-139
ATGCT 1195200 0.43539926 6.6671295 50-59
ATTCT 921600 0.4343269 7.371493 2
GCATG 1532145 0.43143952 8.835929 60-69
TGCTA 1168090 0.42552343 5.8919945 50-59
TGCGC 1719655 0.41629022 8.798362 25-29
TCAAA 992430 0.41227522 5.2873983 200-209
GCCTT 1299390 0.40693167 8.874437 1
ATGGC 1430315 0.40276498 6.9068522 1
GGTCA 1426125 0.40158516 5.1508565 160-169
CTAAC 1107600 0.39555338 5.5424056 110-119
GCGCT 1632550 0.39520404 7.9536667 25-29
CACTT 1036225 0.39415738 7.0025325 3
CCTAA 1084265 0.38721988 7.6489387 110-119
CATGC 1294860 0.38072497 6.930021 60-69
TACCT 997085 0.3792694 5.051838 2
ACGAG 1432830 0.37880987 7.268194 180-189
AGCTT 1029860 0.37516758 7.0885425 1
GGTAA 1137600 0.37262744 7.2865267 120-129
CCGGG 1991280 0.3726148 5.0531707 80-89
TTTGA 818620 0.36947763 5.929592 45-49
GAGGC 1624840 0.35367438 5.408569 35-39
TTCTT 700435 0.35158923 19.092651 1
ATGTG 968445 0.33787313 5.331012 140-149
TGCTG 1118105 0.3353483 14.655659 1
TGGGC 1431100 0.33178473 8.816297 1
CGAGC 1394820 0.31701484 6.11911 180-189
CTCTT 740540 0.30002508 8.63216 1
GCGAG 1344815 0.29272208 5.2349005 240-247
CGCTT 926540 0.29016572 5.6230683 1
TCACA 787480 0.28123006 5.3838444 5
GACTT 768340 0.2798985 6.875252 1
AACTT 631025 0.27920768 10.63518 3
ATTGT 610100 0.27536377 5.3310666 240-247
TTCCT 668250 0.27073726 6.4164224 4
CCCTT 816095 0.2668647 5.736344 3
GAGGT 987420 0.2662892 6.7656507 7
CTCGG 1096160 0.2653559 5.1696377 6
TAACT 581725 0.25739408 7.6035247 2
GTCTT 647280 0.25114977 11.714503 1
AATCT 555640 0.24585232 7.69917 2
TTGGC 811600 0.24341959 6.875663 1
CGGGG 1304825 0.23383613 8.518001 3
TGTAA 550600 0.23331815 7.192923 25-29
CCTCG 877010 0.2216807 5.615674 5
TGCTT 517345 0.20073396 10.830716 1
TCGGT 605225 0.18152246 6.147261 7
TGCCT 575515 0.1802348 5.1567264 4
TAGGC 509010 0.14333305 6.4887614 1
TTGCT 314055 0.121855825 5.987828 4
Produced by FastQC (version 0.10.1)