FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_4_SL248350.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_4_SL248350.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11797639
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 13649360 8.754555 63.04939 110-119
ATACA 12403430 7.5336804 39.388996 110-119
CACAG 18196425 7.505405 42.88091 110-119
TCCCT 21543705 7.160509 109.48975 30-34
CAGTA 15637265 6.810912 41.306534 60-69
AGTAA 11773430 6.384304 48.718987 110-119
GGAGA 18842475 6.1946454 94.54181 35-39
CCCTG 21819980 6.1314983 71.83061 30-34
GTAAT 12952585 5.957408 40.533325 110-119
GGTGA 20940675 5.8392825 102.16089 45-49
AATAC 9070560 5.5093384 41.595707 110-119
GTAGT 15764840 5.1995955 24.957382 70-79
ACAGT 11874180 5.171876 36.1441 110-119
AGACG 13917615 5.1250496 101.17245 40-44
GTGAC 16102275 5.0293384 84.21679 45-49
ACTCC 12356905 4.8422017 125.18065 30-34
CCCCA 12779480 4.7423096 101.33935 60-69
GAGAC 12862975 4.7366867 140.80113 40-44
ACCAG 11437345 4.717515 98.20945 45-49
CTCAG 13339100 4.666647 35.332832 140-149
CACTC 11472840 4.49577 102.6986 25-29
GCACA 10689620 4.4091034 34.40475 110-119
TGACC 12426935 4.347528 143.93304 45-49
GTGTC 16360830 4.3343024 24.381907 120-129
GGCCC 18234130 4.3319774 59.711765 60-69
CGTGT 16326865 4.3253045 23.90593 120-129
TAGTA 9309225 4.281682 31.056097 80-89
CTCCC 13503375 4.2502003 70.98952 30-34
GACCA 10141200 4.1828995 109.96305 45-49
ATGCA 9532440 4.151916 99.152466 25-29
AGTAG 10658520 4.1446376 29.054787 70-79
CCCAG 12085110 4.0038023 86.82524 60-69
CCACT 9897230 3.8783484 28.165977 240-247
TGAGG 13729305 3.8284006 69.20558 35-39
CAGGG 14436340 3.8121455 37.254498 50-59
TACAC 7676385 3.7450361 29.020506 110-119
AGGAG 11350745 3.7316668 95.01865 35-39
ATGGT 11161145 3.6811943 25.567905 190-199
ACGGT 11714945 3.6590118 95.952545 40-44
TGCAC 10456785 3.6582768 119.90916 25-29
GACGG 13819780 3.649333 107.300125 40-44
CTGAG 11673690 3.6461263 105.800446 35-39
GGGTT 15213395 3.5981994 44.33121 50-59
AGATA 6581485 3.5689006 19.483194 150-159
TGTCC 11828975 3.5100703 21.107632 120-129
GAGGA 10668205 3.507275 124.63812 35-39
CCTGA 9923300 3.4716384 87.08892 30-34
CCAGG 11571200 3.4225166 42.37479 50-59
GCCCC 12807165 3.4080732 72.71873 60-69
GCACT 9687870 3.389274 92.924904 25-29
CCCTT 10143570 3.3714314 32.136425 20-24
TGGTG 14240935 3.3681977 17.834263 190-199
GGATG 12072700 3.3664582 72.45251 20-24
TCTTG 10668355 3.3428936 18.140774 170-179
CTTCA 7897435 3.267952 16.714193 200-209
CCTGG 12967850 3.25331 30.776257 50-59
CTCTC 9709120 3.2270327 13.877209 100-109
TCAGG 10292410 3.2147017 21.014343 140-149
CCGTG 12785660 3.2076032 24.043356 120-129
CAGAT 7296455 3.1780183 17.69617 150-159
TTCCC 9553880 3.175435 52.38755 50-59
CTTGG 11957610 3.167804 18.104818 20-24
AGGCT 10132805 3.164851 21.668835 30-34
TCGCA 8997730 3.1478305 24.958258 100-109
TGTCT 9999840 3.1334167 19.51667 180-189
GGTTG 13245335 3.1327236 75.95509 15-19
CGGAT 9975145 3.1156077 82.39405 20-24
CGCAC 9275615 3.0730152 25.67117 100-109
TCTCA 7400975 3.0625174 21.803036 130-139
GGTTC 11476690 3.040399 50.096146 50-59
ACAGA 5896770 3.0280871 15.082466 140-149
GATGC 9642460 3.0116982 99.326546 25-29
AGGGT 10778750 3.0056415 52.41984 50-59
ACCAC 6448975 2.9794323 19.886637 230-239
TGGCC 11710125 2.9377785 44.087753 60-69
GTTGG 12303290 2.9099152 54.394627 15-19
GACTC 8240440 2.8828948 20.772766 190-199
TCAAA 4713935 2.8631818 59.56604 70-79
CCAGT 8173475 2.8594673 38.108845 60-69
AGTCA 6538400 2.8478425 33.59796 70-79
TCTCT 7891985 2.769913 14.522065 180-189
TTCTT 7436320 2.7560961 20.37448 160-169
GTTCC 9286860 2.7557359 56.799294 50-59
GCCCT 9772005 2.7459712 17.50798 20-24
CGGTG 12228410 2.738878 67.61418 40-44
GCCGT 10817795 2.713915 22.54676 120-129
CGGCC 11338190 2.6936727 13.020908 120-129
TCACA 5464000 2.6656919 12.092721 140-149
GATGG 9499570 2.648944 18.100204 15-19
AGGGA 7961675 2.6174774 9.792596 150-159
GCGGA 9859565 2.6035755 90.228516 20-24
AGATG 6684370 2.5992625 17.062206 180-189
GGCGG 13136370 2.487522 43.285015 20-24
TCCAT 5958420 2.465589 15.086364 70-79
TACCA 5008850 2.4436405 16.605318 230-239
TGTTC 7778385 2.4373312 12.967508 140-149
ACTCT 5884855 2.4351478 13.988397 190-199
TTGGG 10281615 2.4317584 82.19105 15-19
CTGGC 9690970 2.4312227 36.416092 60-69
TCCTC 7302630 2.4271846 16.41508 130-139
GGCTG 10758405 2.4096315 11.722341 140-149
ACAGC 5841740 2.4095187 11.041427 120-129
CAGCT 6871970 2.4041395 12.599644 150-159
GTTCT 7598480 2.3809586 18.609982 160-169
TGGAG 8532890 2.3793864 14.758994 25-29
GAGAT 6012740 2.3380947 16.562735 180-189
CACGG 7874095 2.3289912 9.954001 120-129
TGTAG 6964505 2.2970488 8.712867 210-219
AATAA 3014810 2.279772 9.15883 110-119
CTGGA 7286360 2.2758005 14.307619 180-189
CTACT 5497960 2.2750509 7.96527 180-189
CAGAG 6129435 2.2571154 10.111022 140-149
TCAGC 6419900 2.2459838 15.408032 140-149
ACTAC 4584740 2.2367322 12.482063 230-239
CACTA 4573535 2.2312655 15.23044 240-247
CATTT 5088730 2.2235935 17.276024 150-159
TCATT 5070340 2.2155578 15.991518 150-159
TTCAT 5013190 2.1905851 16.412184 140-149
GTGAA 5576730 2.1685493 17.645632 190-199
TAGTC 5862455 2.16578 28.019642 70-79
TGGGG 10824345 2.1644597 54.192547 15-19
TTCAC 5208960 2.155463 18.335857 200-209
GATAC 4918450 2.1422627 14.762791 150-159
TTGGA 6379940 2.1042461 16.181177 170-179
GTCAA 4802075 2.091575 42.72948 70-79
TTGTC 6670950 2.09032 13.291236 170-179
ATTTG 5356190 2.0895228 15.200996 150-159
CTCGC 7427095 2.0870423 16.955181 100-109
AGCTC 5959890 2.0850506 13.360039 140-149
GAACT 4776520 2.080444 11.479855 160-169
TCTGC 6995675 2.0758612 12.0215845 210-219
ATATA 3222570 2.0669222 10.638085 110-119
TCTCG 6848615 2.0322235 16.400333 100-109
GATAT 4398275 2.022941 9.084021 180-189
GAATC 4639260 2.0206597 14.124869 190-199
ACACA 3510385 2.0191274 14.260746 110-119
GCTGT 7612340 2.016657 10.045551 140-149
ATGTA 4372735 2.0111945 7.3006663 150-159
CTCTG 6749525 2.00282 14.240111 180-189
TACAG 4597305 2.002386 13.555357 160-169
ATCTC 4837890 2.0019147 7.809013 100-109
GTCTC 6741165 2.0003393 11.525748 180-189
AACCA 3471070 1.9965136 15.823494 70-79
ACACG 4826965 1.9909585 13.862443 120-129
GTCCT 6691480 1.985596 13.847589 130-139
AAAAA 2224815 1.9835128 7.7235837 8
TGAAT 4309135 1.9819422 13.619237 190-199
GTTCA 5345445 1.9747797 15.232207 140-149
CAGGC 6639380 1.9637885 15.011232 140-149
ACGGC 6636925 1.9630624 14.632785 120-129
AATCG 4483420 1.9527826 13.219494 190-199
ATATG 4237035 1.9487807 9.342152 180-189
AATAT 3037295 1.9480888 13.215611 110-119
TACTA 3760675 1.9374096 9.781812 230-239
CCTTC 5802150 1.9284679 13.790716 200-209
GGGGC 10179365 1.9275792 49.491447 15-19
TTGCA 5217190 1.9273982 14.120716 150-159
TGGAA 4950510 1.9250393 9.314105 170-179
GAGTC 6076700 1.8979788 11.847212 210-219
CAGCC 5717325 1.8941522 10.193477 120-129
CATAG 4338310 1.889579 8.404493 210-219
GGGCG 9886225 1.8720696 60.80121 20-24
TTTGC 5949740 1.8643312 11.951781 150-159
TCGGC 7339090 1.8411946 7.7967477 130-139
AGTAT 3939760 1.812052 11.658845 220-229
AGAAC 3523775 1.8095155 11.910416 160-169
GTTGT 6414905 1.7945704 7.5150986 210-219
CTCCA 4536320 1.7776116 8.217878 150-159
CTGTT 5642755 1.7681385 12.672777 140-149
GACGT 5636500 1.760488 10.368483 170-179
GTCAC 4981755 1.7428529 9.432344 130-139
ACTGA 4001150 1.7427268 9.554569 180-189
CACCA 3732835 1.7245733 13.433178 230-239
TCCCA 4374750 1.7142984 10.69836 240-247
GGTCA 5478500 1.711139 8.189532 130-139
CGTAG 5468890 1.7081372 9.728124 210-219
AGTCT 4619185 1.706476 12.797149 210-219
AACTG 3916575 1.7058895 11.282836 160-169
GCAGA 4615260 1.6995323 14.364169 150-159
TGGTT 6072260 1.6987154 18.668785 10-14
ATTGT 4338900 1.6926641 7.629208 170-179
TGACT 4563170 1.6857822 12.106263 190-199
ATAGT 3665065 1.685709 7.4313426 220-229
GAGAA 3665680 1.6805596 10.836362 160-169
CCAGA 4058855 1.6741391 24.121262 60-69
TGCAG 5353920 1.6722277 13.413772 150-159
CAAAG 3209095 1.6479224 30.789526 70-79
TAGTT 4224165 1.6479048 10.365469 220-229
TTCAG 4456955 1.6465428 9.831192 150-159
TTGTA 4218480 1.6456867 9.735265 210-219
TCAGA 3749190 1.632984 10.328639 130-139
AATAG 3007930 1.6310914 9.900129 240-247
CTGGT 6089295 1.6131729 7.501204 160-169
ATAGA 2972605 1.6119361 10.645664 240-247
TGTGC 6083050 1.6115184 8.140194 220-229
GCTCC 5729580 1.6100341 6.379829 150-159
GTCTG 6067105 1.6072944 8.893661 210-219
GTGCT 6060925 1.6056571 8.592712 220-229
TGCTC 5401190 1.6027218 5.350355 150-159
GCTCA 4545445 1.5902112 11.930816 140-149
CAAAA 2212450 1.5843407 20.555372 70-79
GAACC 3840155 1.583933 12.96525 70-79
CTGTG 5954715 1.5775201 5.3719187 210-219
GGACG 5957045 1.5730528 7.670133 170-179
TCTGG 5906740 1.5648106 11.677948 180-189
CCCAC 4215560 1.5643431 10.321388 240-247
GGCCG 7337890 1.5563867 13.413768 120-129
CCTTG 5243335 1.5558805 20.695345 20-24
TTGGT 5559750 1.5553406 22.214676 25-29
CCACC 4169455 1.547234 9.828668 230-239
CACTG 4421035 1.5466866 7.7941537 230-239
GTCCC 5319585 1.4948239 17.713154 50-59
TGATA 3211330 1.4770181 8.596101 180-189
ACCAT 3022430 1.4745365 10.800382 45-49
CGACT 4168465 1.4583257 9.421011 190-199
GCTCT 4910840 1.4572178 10.079205 130-139
TATAC 2822360 1.4540123 6.900888 110-119
TGGAC 4614840 1.4413859 10.832826 170-179
CTCTT 4042360 1.4187794 8.497095 200-209
TCGAC 4050300 1.416986 10.083773 190-199
GAGGG 6007435 1.416272 8.902859 200-209
GGGAG 5995060 1.4133545 7.0464535 150-159
CCTCG 5017630 1.4099733 9.205257 130-139
AATTG 3051945 1.4037106 9.427601 170-179
CATGT 3753355 1.3866104 6.842307 150-159
AAATA 1833655 1.3865931 5.2586555 9
CTGCT 4625700 1.3726069 6.0868473 90-99
CTGAT 3708020 1.3698621 8.011797 180-189
GCTGA 4357225 1.3609229 18.78872 30-34
CCATC 3453840 1.3534286 10.803315 240-247
CTTGA 3653225 1.3496192 8.8792715 200-209
ACTTC 3255675 1.3471955 6.7814417 220-229
TCCAC 3423090 1.341379 10.2544 240-247
CCTCA 3420575 1.3403933 7.34791 130-139
GCGTA 4288640 1.3395014 10.13845 210-219
CTCGA 3818005 1.3357183 9.019085 190-199
AGATC 3042100 1.3250064 7.534781 130-139
TATGG 3995075 1.3176647 6.6039863 180-189
ACGTG 4196935 1.3108586 9.9957075 170-179
ACGGA 3558020 1.3102121 9.427287 200-209
CAGAC 3172055 1.3083646 13.667099 60-69
GAATT 2833630 1.3032988 10.4021225 170-179
ACTAT 2520740 1.2986249 6.3730583 230-239
AAAGT 2393370 1.2978377 22.676178 70-79
GTAGG 4629045 1.2908038 5.1514173 160-169
ACTCG 3614775 1.2646189 9.254305 190-199
AAGTA 2319755 1.2579191 16.477156 70-79
CCATG 3581910 1.253121 6.635323 70-79
ATCGG 4011055 1.2528014 7.820437 190-199
GTGTT 4469705 1.2504004 7.5195384 160-169
ACTGG 3999720 1.249261 7.6100974 160-169
GGAAT 3209060 1.2478648 8.719054 170-179
GTGGA 4463840 1.2447364 19.3369 25-29
AGCCG 4207275 1.2444232 8.997697 120-129
ATAAC 2042930 1.2408488 7.8052793 80-89
GGAGT 4432960 1.2361257 6.8986053 210-219
TTGAG 3730085 1.2302651 8.610288 200-209
AGCTT 3317515 1.225597 6.5683465 150-159
GATCT 3306180 1.2214096 5.5988836 130-139
GGCTC 4865970 1.2207507 8.292791 130-139
TTCCA 2925505 1.2105714 7.6984215 230-239
AGAGC 3270290 1.204258 7.8790436 140-149
ATCCA 2467925 1.2040131 16.313826 240-247
GGAGG 5103160 1.2030864 14.422365 30-34
GCATA 2740405 1.1936011 8.106047 210-219
ACTGT 3217540 1.188663 5.9381733 230-239
ATACC 2360110 1.1514142 6.5572424 70-79
ACCGT 3286660 1.1498288 15.995482 45-49
GTGGT 4859190 1.1492723 9.127298 50-59
ATAAA 1516940 1.1470964 5.1591377 7
TCTAC 2761625 1.142758 7.613061 180-189
GTCCA 3245215 1.1353294 14.2033 70-79
GTCTA 3062840 1.1315119 7.649086 180-189
TTGGC 4268710 1.1308644 7.27289 60-69
TCACG 3225485 1.1284268 9.451655 200-209
TATCA 2189955 1.1282123 6.4852514 70-79
TGGTC 4257930 1.1280086 10.398845 50-59
GCTTC 3795290 1.1261952 5.933296 150-159
TGCCC 4004965 1.1254107 5.9027047 60-69
TACTG 3040055 1.1230943 6.990039 180-189
TCTGT 3549345 1.1121755 6.5530167 210-219
AGGTT 3367980 1.1108347 23.865839 10-14
AGTTC 3003815 1.1097062 5.4902124 160-169
CAATA 1815315 1.1025983 6.7668147 100-109
GAGCT 3522020 1.1000575 6.998146 140-149
AAAAG 1698510 1.0858964 13.503701 70-79
AGGGG 4590040 1.0821166 5.1901603 60-69
CTACC 2744310 1.0753908 8.6225195 230-239
TATGT 2738545 1.0683439 7.5010962 220-229
TAGAT 2320860 1.0674556 8.206068 240-247
CCATA 2179100 1.0631055 9.073544 70-79
GGGGT 5302240 1.0602477 8.305186 10-14
CGGCT 4222480 1.059315 7.735873 130-139
ATATC 2042500 1.0522472 7.0252867 70-79
AACTT 2026365 1.0439346 5.9706144 200-209
CTGCC 3707815 1.0419103 5.7852774 200-209
ACCCA 2253930 1.0413177 13.456973 240-247
CTGCG 4149735 1.041065 6.772186 210-219
GTCCG 4142535 1.0392587 5.2636724 120-129
CTCGG 4136840 1.03783 8.172156 130-139
GAAGA 2258815 1.0355713 16.05961 35-39
CTGAA 2367585 1.031217 13.34506 35-39
CATGG 3300170 1.0307657 5.291338 190-199
GCGGT 4593795 1.0289028 6.0849237 130-139
CCAAT 2079050 1.0142946 11.505354 240-247
ATACG 2319400 1.0102298 5.0638857 120-129
CTTCT 2875105 1.0090985 5.796676 210-219
AGAGA 2173465 0.996442 11.971457 35-39
TATAA 1538515 0.9867871 6.413026 240-247
TGCGT 3718330 0.98505807 8.1049185 210-219
TGTAA 2129065 0.9792415 7.2262664 230-239
AAAAT 1293545 0.978167 5.7014194 8
CGAAC 2369910 0.97750705 13.59371 70-79
ATGGG 3490195 0.9732368 28.041985 15-19
AGCTG 3105945 0.9701019 5.187722 90-99
CAGCG 3261715 0.9647465 6.2265363 160-169
ACTAA 1564255 0.9501078 12.588006 240-247
CCCAA 2056180 0.9499571 10.665972 240-247
CGGAG 3553900 0.93846387 6.3846164 210-219
AGTTT 2395500 0.9345174 5.0008116 220-229
TAGGC 2967290 0.9267948 5.806563 160-169
CTGTA 2503100 0.9247259 5.0699 230-239
ACAAT 1504970 0.9140989 7.7054625 100-109
AAGAG 1983225 0.909225 11.999092 35-39
CTATA 1761405 0.9074336 5.157116 90-99
GAGTT 2750890 0.9073047 5.105165 160-169
TCGAA 2074610 0.9036098 16.960226 70-79
TTCTG 2875290 0.9009625 5.7491727 210-219
CCATT 2176070 0.9004558 5.414384 50-59
TGCAT 2436825 0.9002417 6.1264153 210-219
CCAGC 2682815 0.88881767 6.022811 80-89
CTTCC 2646875 0.87974524 5.4589496 230-239
CATCA 1785710 0.87118465 5.6401567 230-239
CGTGG 3854575 0.8633347 6.2864156 50-59
TGAAC 1981965 0.86325765 6.499358 190-199
CGGTC 3404820 0.85418445 5.9014263 130-139
TGAAG 2176100 0.8461912 8.795436 35-39
GTATG 2519030 0.8308322 5.3009467 220-229
GGTGG 4145330 0.8289094 19.38487 25-29
AGCGT 2643580 0.82568806 6.4697356 160-169
ATCAA 1324940 0.80475104 12.036451 70-79
TCCAG 2283245 0.7987869 5.1079564 240-247
GAGGC 3016895 0.7966592 12.19006 30-34
AATCC 1629435 0.7949436 10.387701 240-247
TAACC 1628635 0.79455334 6.3404937 80-89
CAATC 1617510 0.7891258 10.267049 240-247
ACGTC 2249705 0.7870529 9.584702 70-79
AAAGA 1227735 0.7849191 5.9415812 8
GGTGC 3496025 0.78302795 5.1552243 220-229
AACCG 1887335 0.7784613 5.8015413 190-199
AAAGG 1696190 0.7776316 9.179452 10-14
AGCCA 1843980 0.76057893 6.286421 80-89
CTAAT 1468695 0.7566365 9.597891 240-247
TGAGA 1943135 0.7556012 5.6143665 240-247
GGGCC 3559030 0.75488 14.614409 15-19
ATGAT 1637795 0.7532869 6.993554 240-247
GTCGA 2351630 0.73450124 12.363256 70-79
AAGGT 1853710 0.7208277 10.079305 10-14
CGTCC 2520725 0.70833343 7.8693695 70-79
GTAGC 2267560 0.7082432 5.51628 80-89
TGGGC 3130725 0.70120925 15.967696 15-19
GGTCC 2712810 0.68057644 10.63859 50-59
GACCG 2298710 0.67990994 14.907197 45-49
GGAAG 2022010 0.6647553 5.910264 30-34
TATGA 1438605 0.6616715 5.570518 240-247
TAGGT 2002985 0.66062903 5.5273004 10-14
CATAC 1350945 0.6590782 7.798073 70-79
AGACC 1584025 0.6533563 5.170902 240-247
AAAGC 1257365 0.64567727 9.226597 70-79
GAGGT 2256095 0.6291094 7.0294714 10-14
AGGAT 1606640 0.6247528 5.2487273 10-14
ATAGG 1593915 0.6198047 5.4183702 10-14
AGAAG 1318855 0.60463935 5.2211385 9
CATCC 1498460 0.58718956 7.304867 240-247
AATGG 1500315 0.58340764 6.4831967 10-14
GGGTC 2596865 0.5816371 5.900828 70-79
CGATG 1811620 0.56583613 12.380438 15-19
AAATG 1043020 0.5655919 5.0469465 9
GAAAA 868825 0.55545974 6.4409933 8
TAAAA 727980 0.5504919 6.2967978 8
GAAGG 1617530 0.5317786 5.4632816 10-14
AAGAA 829080 0.5300498 5.5593987 9
AAGGG 1594125 0.524084 6.443537 10-14
TGATC 1383035 0.51093775 5.4227657 240-247
GGTCG 2228220 0.49906924 6.064865 70-79
GATCC 1420630 0.4970034 5.4839544 240-247
AAGCA 960400 0.49318096 6.0107784 70-79
ATAAG 854025 0.46310687 6.2097216 9
CGGTT 1695735 0.44923323 8.273452 10-14
ACGAT 1005255 0.43784535 5.523036 10-14
TAAGG 1117140 0.43440747 5.891659 10-14
TAAAG 762330 0.413384 5.6022124 9
TGGGT 1668190 0.39455232 5.157692 10-14
AAGCT 903725 0.39362326 8.020205 30-34
GAAAG 832730 0.38177156 5.156016 9
Produced by FastQC (version 0.10.1)