FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_6_SL248352.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_6_SL248352.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11106874
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 53932 0.4855731684720651 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 38849 0.3497743829631992 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 38152 0.3434989899048103 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 37673 0.3391863453209247 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 37055 0.333622223498709 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 36827 0.3315694406905129 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 36146 0.3254381025660325 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 33159 0.2985448470919901 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33145 0.2984187990248201 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 33019 0.2972843664202907 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 32845 0.2957177690140358 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 32502 0.2926295913683724 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 32377 0.29150416219721226 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 31660 0.28504870047143777 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30898 0.2781880842440456 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30134 0.27130946114991494 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29814 0.26842836247174495 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29346 0.2642147556549214 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 28744 0.25879468876661427 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 28439 0.25604864158898355 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 28367 0.25540039438639534 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 28324 0.2550132467515162 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27159 0.2445242468763038 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26861 0.24184122373225805 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26506 0.2386450048861633 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25861 0.23283779036297703 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24549 0.22102528578248026 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24039 0.21643353476414698 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23529 0.21184178374581364 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23280 0.20959992883686265 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22838 0.20562041128764041 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 22684 0.20423388254877115 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22317 0.20092962250224503 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22309 0.20085759503529077 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22122 0.19917395299523522 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22061 0.19862474355970905 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21846 0.19668900538531361 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21431 0.19295258053706202 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21419 0.19284453933663062 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21194 0.1908187668285424 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20908 0.18824378488492802 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20763 0.18693828704638227 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19494 0.17551293010076463 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 19347 0.1741894253954803 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 19262 0.17342413355909142 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 19075 0.17174049151903586 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19074 0.1717314880856666 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 18393 0.1656001499611862 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18291 0.16468179975751954 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17997 0.16203479034695092 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 17908 0.1612334847770849 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 17698 0.15934276376953588 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17428 0.15691183675982998 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 17380 0.1564796719581045 No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG 17350 0.1562095689570261 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17293 0.15569637325497707 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16485 0.14842159909259797 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 16332 0.14704407378709797 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15127 0.13619493657711432 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 15123 0.13615892284363718 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15094 0.13589782327592803 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14727 0.1325935632294019 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14225 0.12807383967802283 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13906 0.12520174443322216 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13802 0.12426538736281693 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13057 0.11755782950270256 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12557 0.11305611281806204 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11848 0.10667267855924177 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11633 0.10473694038484636 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 22795565 7.758127 66.90237 35-39
TATTA 11365685 7.524627 130.1422 240-247
TGGAT 17224905 7.2951803 80.20424 35-39
TCACC 17639465 6.4739428 75.18174 160-169
TCTCC 15620370 6.124731 86.021935 20-24
CTGTG 16484345 5.9936376 87.91681 25-29
TCCCT 14958175 5.8650846 73.38183 15-19
AGTGG 17831785 5.844036 40.60437 100-109
CTGAG 16698380 5.683042 50.581764 15-19
TGTAT 10336115 5.6571507 60.602028 240-247
TTACT 9610075 5.462049 114.71369 240-247
ATTAC 9614090 5.114755 108.01762 240-247
TGGGT 14179735 4.964762 46.437294 100-109
CCTGA 13859435 4.898246 52.00969 15-19
CTCTG 12635110 4.7707458 74.767204 35-39
TTCAC 10358405 4.730957 76.58315 40-44
ACACG 14206980 4.6998553 50.020626 230-239
CTCCA 12697405 4.6601343 41.686172 80-89
GACAC 14070775 4.654797 47.116154 230-239
TGAGA 11401545 4.51992 95.85829 15-19
ATTCA 8262960 4.395945 114.91426 40-44
TACTG 9971820 4.3857327 80.514046 240-247
AAGGG 14281030 4.380921 40.209053 90-99
TCTGG 11848150 4.3079376 137.22008 35-39
GAAGG 13756580 4.2200375 31.976576 90-99
TGGAG 12864420 4.216074 39.634598 90-99
CCCTG 13795270 4.185667 85.60905 10-14
AGCCT 11824320 4.1789885 52.159252 30-34
CAGCC 14614225 4.1504774 56.429276 30-34
CCAGG 15146630 4.1423793 54.025707 80-89
ACCAT 9652460 4.1265006 68.528244 160-169
GAGAC 12860880 4.0969906 89.64648 8
TCAGT 9293865 4.087559 96.39147 7
ACTAC 9486675 4.0556264 36.56725 130-139
TACTA 7547560 4.015348 29.888302 130-139
GTATT 7323435 4.008254 70.334885 240-247
CACCA 11665990 4.007678 58.057304 160-169
TCCAG 11314920 3.9989548 41.157486 80-89
GGGTC 14008300 3.941332 126.7499 8
ACTGT 8768985 3.8567104 69.44955 240-247
CACCT 10423795 3.825686 44.129086 20-24
TGAAG 9561165 3.7903368 40.656628 150-159
CTGGG 13450315 3.7843397 133.96114 3
GACTC 10686350 3.7768035 52.305862 20-24
AGACT 9124770 3.7564478 70.01032 15-19
GATTC 8525835 3.74977 60.31411 40-44
CAAGA 9697725 3.7369134 45.69724 180-189
GGACA 11434265 3.6425254 28.845528 220-229
GCTGG 12809840 3.6041374 33.73988 90-99
ACTGG 10517095 3.579335 44.988586 60-69
CCTGT 9304670 3.5132437 61.68968 25-29
ACTCT 7559195 3.4524844 68.89805 20-24
GAGTG 10439545 3.4213665 39.46524 90-99
TATCT 5992680 3.4060416 50.041676 200-209
AGACA 8718230 3.3594756 39.252666 170-179
GGATT 7918195 3.3535552 57.732475 40-44
AGCTG 9775830 3.3270562 38.14309 60-69
TCCTG 8801900 3.3234081 63.31334 20-24
CTCCT 8274715 3.244507 92.72256 20-24
CTGCA 9138410 3.2297256 46.38301 25-29
GGTCC 11014055 3.2180536 142.24362 9
CATCT 6962750 3.180072 33.00826 170-179
GTCTC 8308460 3.1370957 34.06363 30-34
CCATC 8538310 3.133685 36.46048 45-49
GTCCC 10102215 3.0651455 74.96184 10-14
AAGAA 6788475 3.047024 39.386585 180-189
GGAGT 9273140 3.0390997 37.971252 90-99
CCTCA 8279900 3.038845 80.963425 5
AGTAG 7573190 3.002243 30.239689 50-59
GGCTG 10472355 2.9464698 34.887295 90-99
AGGGA 9599630 2.9448307 41.58296 80-89
GAGCT 8603400 2.9280374 31.093748 60-69
TGTGC 7822845 2.8443532 58.37047 25-29
GGAAG 9173325 2.8140554 31.18245 80-89
ATCTG 6363335 2.798675 37.63101 200-209
GTGAA 7033900 2.788452 39.663136 150-159
CAGGG 10538875 2.7754893 37.206665 80-89
GACAA 7173005 2.76404 25.181784 180-189
GTGTA 6489535 2.7484815 37.1473 230-239
ATCTC 6009995 2.7449236 48.61245 170-179
GAACA 7117210 2.74254 35.860424 210-219
ACCTT 5978875 2.7307103 72.3368 40-44
CTATG 6171175 2.7141607 28.445532 50-59
ATGAA 5594825 2.6828873 28.355787 210-219
GGGAA 8643715 2.6515894 34.756413 80-89
AGAAC 6790600 2.6166842 31.467112 190-199
GAGTC 7638185 2.599541 29.033716 150-159
CACGG 9494285 2.5965464 44.757942 230-239
TGCAA 6210215 2.5565958 36.478264 200-209
ATCAG 6202900 2.5535843 28.49261 45-49
CCCTC 8095825 2.5508451 27.331669 20-24
CGCCA 8940440 2.5391078 39.84463 70-79
TATAT 3819600 2.528758 25.57754 240-247
AGGGC 9580010 2.5229654 33.06688 150-159
CCAAG 7613060 2.5185 39.95633 180-189
CAGTG 7351820 2.502081 74.542694 8
CTACA 5842020 2.4975083 19.61112 190-199
AGGCT 7280145 2.4776876 20.303963 80-89
ACGGC 9057205 2.4770112 49.561783 230-239
GTGGA 7429005 2.4347184 27.208296 100-109
CACTG 6844985 2.419176 20.433151 30-34
TCTGT 5139825 2.4150622 25.564148 210-219
GCAGC 8794155 2.4050713 35.01206 30-34
CTGTC 6359685 2.4012802 50.233044 30-34
CTACT 5240115 2.393299 23.534203 50-59
TGAAC 5747675 2.3661792 38.742237 210-219
CCGCC 9602040 2.3411288 33.996235 70-79
TATGC 5312045 2.3363047 21.879612 50-59
CTCTC 5957880 2.3360786 97.806496 20-24
TCCAA 5446280 2.3283265 44.202602 180-189
CAAAT 4670200 2.325626 36.04687 200-209
GGGCT 8247680 2.3205419 42.838215 90-99
ACAGC 7011275 2.3194218 27.4366 210-219
GTATC 5239450 2.3043764 23.284622 200-209
CTTCA 5041315 2.3025022 50.66949 45-49
GTGGT 6569840 2.3003035 32.27901 120-129
GGCCG 10089745 2.2812092 25.608992 230-239
TGGTG 6513265 2.280495 35.402378 35-39
CAGTA 5501155 2.264693 22.421072 45-49
CAGGC 8247990 2.255703 33.431637 70-79
AATGA 4693465 2.250658 26.596388 210-219
AAATG 4684950 2.2465746 34.337513 200-209
ACAAC 5610195 2.244965 20.363363 180-189
GCCTC 7336390 2.2259579 71.36894 4
GCTCC 7303605 2.2160103 34.18637 80-89
GTAGT 5209950 2.206545 26.456202 120-129
GCCTG 7459865 2.1796014 30.807207 210-219
TACAT 4094250 2.178166 18.500097 190-199
GCTAT 4903500 2.1566212 34.817127 50-59
GGGGG 10274350 2.1540825 95.900795 5
GTGGG 7946010 2.1528723 30.58796 100-109
TATCA 4046705 2.1528718 17.891819 170-179
CCTTC 5420935 2.1255429 55.154613 45-49
TGCAG 6172165 2.1006033 44.30325 30-34
GCAGA 6573595 2.0940993 24.914223 140-149
GCTGT 5757570 2.093428 19.476906 230-239
TCTAT 3676945 2.0898542 21.106207 110-119
CCTCT 5303050 2.0793204 49.337677 35-39
AACAG 5331745 2.0545304 34.515263 210-219
AACTA 4116145 2.0497222 37.811962 130-139
TGCAC 5746520 2.0309532 27.554348 25-29
AGCAC 6126725 2.026801 21.533447 130-139
CTCAC 5519075 2.0255816 55.562706 20-24
TGAGC 5931145 2.018576 18.339912 200-209
TGGTA 4750415 2.0119205 32.050343 120-129
TGTGA 4748535 2.0111244 19.816332 210-219
ATATC 3778100 2.0099723 14.219174 160-169
CAGAG 6294825 2.0052936 33.039207 170-179
GGCTC 6828895 1.9952463 20.820015 40-44
ATGGA 4982405 1.9751772 12.233752 100-109
CATAT 3711650 1.9746203 15.012581 160-169
AGAGT 4963980 1.9678726 31.49589 150-159
TCCGC 6435205 1.9525265 42.11264 70-79
CTGTA 4421810 1.944768 25.229885 190-199
CCGTG 6654090 1.9441723 20.449953 230-239
TCTCT 3982175 1.9430715 33.791264 35-39
CAGAC 5859875 1.9385235 26.98286 140-149
TTCAG 4395070 1.9330072 47.637054 45-49
GGTCT 5299085 1.9267246 35.293427 15-19
CTATT 3342200 1.8995962 21.055742 110-119
TCCAT 4156345 1.8983127 28.123201 45-49
GGTGG 7002305 1.8971871 18.874485 40-44
GGATC 5551760 1.889458 18.598984 70-79
GCACA 5707675 1.8881739 18.966887 130-139
CCAGA 5694770 1.8839047 34.54321 170-179
ACCAG 5689485 1.8821563 14.449078 190-199
CAGTT 4276830 1.8810037 25.81604 190-199
GCCAG 6876595 1.8806471 35.662136 70-79
ACTAT 3533980 1.8800988 17.410255 130-139
TGGGG 6928330 1.8771446 124.80934 4
AGAGA 5053095 1.8750473 28.859993 170-179
GTCAC 5282595 1.8669914 38.369804 160-169
AGGAC 5847825 1.8628962 21.048826 220-229
TGGGA 5667905 1.8575505 16.734692 100-109
GCTGA 5438030 1.8507516 27.593243 200-209
ACCCT 5022495 1.8433294 43.880196 10-14
GATGG 5603715 1.8365134 20.682716 100-109
CCTGC 6036040 1.8314145 49.299038 20-24
TGTCT 3896715 1.830959 51.36269 30-34
ATACT 3440380 1.8303031 27.824295 130-139
TAGTG 4304190 1.822933 27.113945 120-129
GAGGA 5900830 1.8101683 27.511885 220-229
TTCCA 3960315 1.8087807 23.349184 180-189
GCCGA 6610125 1.8077713 19.878824 220-229
TAGTA 3525995 1.8063831 17.716537 120-129
TCAGC 5091440 1.7994328 11.664329 45-49
CACAG 5403630 1.7875917 15.873706 140-149
TACAA 3563860 1.7747002 18.556417 130-139
AGTCA 4299410 1.7699635 29.65395 160-169
AGTTC 4005970 1.7618762 22.98805 190-199
CAAGG 5525055 1.7600739 17.251793 80-89
GCAAA 4554255 1.7549331 33.037796 200-209
CATGA 4242325 1.746463 24.22489 60-69
AGCTA 4236425 1.744034 26.771076 50-59
ACCTG 4905105 1.7335777 31.139475 25-29
CTGAA 4195630 1.7272397 24.230906 200-209
TGACC 4862350 1.7184671 14.63788 210-219
GGAGC 6500515 1.7119582 13.072649 200-209
GTGCA 5023770 1.7097644 51.232246 25-29
CTCAG 4791765 1.6935207 77.677635 6
CCTGG 5771615 1.6863335 35.144688 2
TTCTG 3582610 1.6833698 42.431465 30-34
ATGCA 4086890 1.6824741 16.520758 60-69
ATTGG 3955480 1.6752455 21.457457 100-109
AACAC 4179290 1.6723769 23.101892 190-199
GGATG 5063570 1.659491 22.120052 100-109
AGGGG 6421250 1.6284577 33.608025 90-99
TCTGC 4277375 1.615045 33.77488 200-209
TCTCA 3531100 1.6127468 27.241024 100-109
CAGCT 4543850 1.6059018 13.219296 50-59
GCCGT 5496150 1.6058488 29.885883 230-239
GGGGC 7332335 1.5963892 29.05032 90-99
GGGCC 7025240 1.5883496 27.949064 2
CGATT 3611260 1.5882779 35.233353 160-169
CTCCC 5038100 1.5874124 14.173949 190-199
AGTTA 3094755 1.5854568 16.991318 50-59
GTTCT 3348150 1.5732034 24.226316 190-199
GCACT 4420590 1.5623391 26.66713 25-29
TTCTC 3197490 1.5601904 22.959404 190-199
ACAAT 3130780 1.5590388 23.789518 180-189
TCTGA 3528470 1.5518658 21.297478 220-229
CGTCC 5064605 1.5366681 19.365961 180-189
CCATG 4313965 1.5246551 15.623606 160-169
TTAGT 2782810 1.5230843 26.41131 110-119
GAGCA 4767275 1.5186738 7.767283 200-209
CGGCC 6446505 1.5135533 31.926477 230-239
GTTAC 3422475 1.5052478 16.699282 50-59
CTTTA 2643075 1.5022367 43.472824 45-49
GGCTT 4126815 1.5004923 21.3882 25-29
AGTGA 3780790 1.4988203 86.37163 9
CATCA 3497270 1.4951097 32.688 45-49
GTCCG 5100375 1.4902124 34.429028 70-79
ACTCC 3957000 1.4522772 17.393803 140-149
CTGGT 3978230 1.4464675 32.56561 35-39
TAGCA 3508500 1.4443649 12.185517 130-139
CTCAA 3349670 1.4320097 19.179132 150-159
GTGAC 4194860 1.4276572 15.7229185 210-219
TACGC 4024410 1.4223197 24.915121 140-149
GTCCA 3970245 1.4031765 29.24511 180-189
AAGCT 3405780 1.4020776 17.616394 200-209
GGCAG 5316060 1.4000232 13.530653 150-159
CAACC 4071960 1.3988614 15.696732 140-149
GGGGT 5156505 1.3970907 119.48189 7
CAACT 3240805 1.385469 18.293709 130-139
AACCC 4010585 1.3777769 20.877447 140-149
GAGAG 4472400 1.3719759 26.49629 220-229
ATCTA 2575785 1.3703333 21.717606 110-119
AGCAG 4292355 1.3673822 13.143304 50-59
CGTGT 3752715 1.3644712 24.436022 230-239
GAACT 3286320 1.3528987 14.900456 190-199
CTACG 3820965 1.3504175 16.408878 130-139
CCATA 3151285 1.3471985 15.487923 160-169
CACGT 3795825 1.3415325 16.989735 180-189
CAATT 2517415 1.33928 24.892408 180-189
TCAAG 3238930 1.3333894 24.188732 150-159
GGCCT 4554245 1.3306458 68.504166 3
TGGAA 3352045 1.3288527 19.798214 120-129
AAGAG 3574820 1.3265052 19.826468 150-159
ACAGT 3220025 1.3256067 12.680365 120-129
CCTTT 2709880 1.3222649 49.87604 45-49
TTACA 2484140 1.3215775 10.101986 120-129
CGACA 3992735 1.3208493 16.208134 70-79
GTGGC 4671890 1.3144686 14.926509 40-44
AACCA 3269095 1.308155 18.012337 190-199
TGTCC 3454980 1.3045261 54.99802 15-19
TGTGT 2880900 1.3035259 15.435252 240-247
GTGCG 4623655 1.3008972 17.36441 240-247
AATTC 2445220 1.300872 24.880848 180-189
ATACA 2605110 1.2972702 21.969002 35-39
CCAGT 3667065 1.2960256 19.624035 190-199
AGAGC 4053890 1.2914163 25.434975 220-229
TTATT 1822485 1.2890376 20.243881 240-247
ACATA 2588390 1.288944 21.810259 130-139
ACATG 3123730 1.2859644 14.531787 190-199
TCCGT 3393695 1.2813864 13.951062 150-159
TGCGA 3759045 1.2793344 14.581277 240-247
ACAGA 3318045 1.2785728 20.124672 140-149
TCACT 2799160 1.2784504 13.678378 190-199
ATATT 1925200 1.2745744 27.189661 240-247
GATTG 2986650 1.2649215 24.6951 100-109
AGACC 3817310 1.2628162 101.64938 9
ATCCG 3567075 1.2606869 24.50657 70-79
ACGTC 3537200 1.2501284 18.792418 180-189
ATTAT 1870960 1.238665 9.596529 50-59
CCTAC 3365010 1.2350081 17.01865 190-199
AGTCG 3624135 1.2334197 16.69508 150-159
ACACA 3081765 1.2331934 7.6508183 130-139
CGGCT 4176605 1.2203081 21.205 230-239
CTTCT 2490685 1.2153105 22.689926 30-34
ATTAG 2335030 1.1962465 26.087608 110-119
GTCGA 3512260 1.1953448 12.967832 150-159
TACCA 2788175 1.1919662 10.807704 45-49
TGGAC 3493020 1.1887966 15.8764 80-89
GATCC 3360575 1.187705 24.331528 70-79
TCGGA 3464210 1.1789917 95.798874 5
TGAGT 2771705 1.1738869 20.900084 90-99
ACGCA 3533735 1.1690059 21.478632 140-149
ATCAT 2195635 1.1680912 15.898405 110-119
CTTCG 3083800 1.1643767 107.152756 3
GAAGT 2935770 1.1638285 13.668171 140-149
ATTCC 2543225 1.1615582 22.886106 180-189
AGCTC 3282110 1.1599736 12.847739 210-219
CGCTG 3956865 1.1561052 12.75899 190-199
GGACT 3379990 1.1503288 13.5347595 90-99
TCGAG 3373305 1.1480535 12.61686 150-159
GTATA 2238850 1.1469729 10.679619 240-247
TTGGG 3272540 1.1458172 16.017979 100-109
GGGTG 4221775 1.1438372 14.476785 60-69
AAGGC 3570145 1.1373135 28.671896 25-29
GAACC 3418695 1.1309491 10.949743 180-189
GACCC 3948355 1.1213429 43.178005 10-14
GATCT 2543770 1.1187822 9.2596445 220-229
TTCGG 3059560 1.112443 103.267235 4
AGGCC 4067330 1.1123545 14.481937 2
GCAAG 3481020 1.1089218 16.584421 25-29
TAGAC 2689500 1.1072024 18.22428 170-179
GAAGC 3473780 1.1066151 13.738912 200-209
GCCCC 4519065 1.1018194 12.400945 80-89
ACAAG 2859125 1.101733 17.534843 80-89
CGCAG 4023200 1.1002858 16.898893 140-149
CACAT 2559665 1.0942764 14.385099 130-139
GCACC 3844270 1.0917827 11.482203 130-139
CCGAT 3084850 1.0902574 23.603785 160-169
CCCCT 3450785 1.0872787 9.99768 140-149
AGGTC 3176610 1.0811113 18.154598 10-14
CTTGA 2438020 1.0722721 22.114763 90-99
TTGAG 2530640 1.0717897 20.640636 90-99
GCTTC 2836240 1.0709032 27.38859 2
ATGAG 2687385 1.0653613 20.955812 60-69
CTATA 1993680 1.06065 13.765849 50-59
TAGTT 1935160 1.0591496 15.922586 50-59
ATCAA 2119905 1.0556519 12.655775 110-119
ATGGG 3213540 1.053178 19.732683 100-109
TGGCT 2893105 1.0519205 32.69746 40-44
GGAGA 3420615 1.0493251 86.08038 7
GAGCC 3833650 1.0484464 18.508844 220-229
TACAG 2541565 1.046301 9.451932 120-129
GCTCT 2770735 1.04617 18.057558 210-219
CTCCG 3439315 1.0435337 9.971796 140-149
GTAGC 3063840 1.0427318 11.650537 50-59
GGGAG 4099785 1.0397238 14.095302 5
CAGCA 3142480 1.0395737 12.51574 45-49
ACACC 3009395 1.0338329 12.74441 40-44
CGTGA 3037045 1.0336125 20.9905 150-159
AGATC 2508965 1.0328803 12.712886 210-219
ATGGT 2424935 1.027021 9.747649 35-39
CGAGG 3856005 1.0155071 15.040276 220-229
AGCCG 3710710 1.0148243 19.502592 220-229
ACCGC 3567870 1.0132842 11.311307 220-229
GTAGA 2552605 1.0119303 18.013208 170-179
TTTAG 1833485 1.0035009 31.60562 45-49
GACAG 3145090 1.0019069 15.621514 70-79
TTATA 1507525 0.99805355 13.764612 110-119
ACCAA 2493810 0.99791837 17.733166 130-139
CGAGT 2922680 0.9946901 15.404142 160-169
AAACT 1992820 0.9923673 17.171509 130-139
ACTCA 2316200 0.99019325 12.59212 190-199
TTTCA 1732545 0.9847214 7.279156 100-109
TGAGG 3000905 0.9834908 11.779755 220-229
GCCAA 2970155 0.98256636 15.109129 180-189
AACTC 2291360 0.97957397 16.511284 190-199
CCAAC 2843075 0.9766962 14.578515 130-139
GGTAT 2296155 0.9724794 9.631329 110-119
ATGAC 2359405 0.97131026 10.96335 160-169
CCGAG 3536620 0.9672132 18.417414 220-229
AAGGT 2416585 0.95800793 20.220713 15-19
ATGAT 1866035 0.95597816 9.130804 120-129
CATAC 2234880 0.9554283 14.717746 130-139
GGGAC 3622205 0.95393413 10.400256 90-99
AAAAA 1749480 0.94986427 5.098396 190-199
GCCAT 2681360 0.94765484 8.041515 60-69
AGCCC 3311975 0.9406094 11.752599 80-89
CCGTC 3099910 0.94055367 10.200195 140-149
CGGAC 3433680 0.9390607 10.940736 220-229
CAGAA 2427700 0.935488 18.15947 140-149
GCAGT 2741370 0.932984 6.4279547 50-59
GATAC 2248530 0.9256656 23.7005 35-39
ACAGG 2893635 0.9218028 15.160891 70-79
TATGG 2172325 0.9200344 12.59561 35-39
AACTG 2232500 0.9190664 11.7504015 60-69
CCCAG 3231305 0.91769904 15.12637 80-89
TTTAT 1295875 0.91656804 10.586706 240-247
CCCCA 3100550 0.91442794 17.211935 80-89
AATCA 1836150 0.9143501 14.147566 110-119
TCATA 1713350 0.9115127 8.999998 110-119
TACAC 2130560 0.91083074 21.993343 35-39
TGGTT 2008380 0.90873516 9.429182 50-59
TAACA 1820795 0.90670377 8.61846 120-129
AAGTT 1762890 0.90313643 17.68752 140-149
GACTG 2644540 0.9000293 13.189581 90-99
GTTAT 1632335 0.89340776 11.164897 110-119
GAGAT 2236870 0.88676333 12.191778 210-219
GGTAG 2702045 0.88554496 12.64121 120-129
CGTAT 1997015 0.8783124 7.9116383 240-247
CGGAG 3331455 0.87736297 74.097916 6
AGAAG 2345725 0.87042594 16.019176 140-149
TCCTT 1770370 0.86383826 10.546229 1
ACGCT 2433190 0.8599457 15.7934065 190-199
TATTT 1207165 0.85382384 10.411506 240-247
ATAGT 1666020 0.85350955 15.92062 120-129
GATGA 2132100 0.8452294 6.7261586 60-69
ATCCA 1961080 0.8383767 9.696833 180-189
GCCTA 2361925 0.83475906 16.418385 190-199
TTACC 1827655 0.8347383 9.703428 45-49
TCTTC 1709015 0.83390063 46.56953 2
GGTTC 2289085 0.8323015 11.680042 160-169
GTTTC 1765835 0.82971716 5.0649137 100-109
AGTCT 1879550 0.8266498 14.666065 8
GATCA 2005770 0.82572716 9.106814 110-119
CATGG 2425220 0.8253871 8.519108 200-209
GTCTG 2238745 0.81399816 14.641526 3
TGGCA 2376925 0.80895054 5.787904 100-109
ATATG 1578455 0.8086496 5.9139423 50-59
GCTTG 2224005 0.80863875 16.912544 90-99
GTCTA 1836705 0.8078061 10.183536 30-34
ATATA 1301240 0.8063702 11.078761 130-139
CCTCC 2559235 0.8063677 10.913724 140-149
CTATC 1764910 0.80608106 8.072955 110-119
ACATC 1877540 0.80266273 5.9203396 110-119
GGTGC 2834165 0.7974119 11.586548 60-69
TCATC 1741260 0.7952795 10.275527 110-119
ACAAA 1697215 0.7910957 12.905036 130-139
TATAC 1479700 0.7872095 9.194572 130-139
TTATG 1422405 0.77850914 11.351575 50-59
CATTA 1444805 0.7686451 12.444833 110-119
GTTCA 1747485 0.768566 14.178977 160-169
TGATG 1813100 0.7678935 11.92119 120-129
GGTTA 1777595 0.75285625 8.372914 50-59
CCCCC 2948470 0.7465295 13.573516 80-89
CCAGC 2611385 0.74164003 6.2784047 80-89
GCGGA 2806550 0.73912543 9.301546 220-229
CTGAC 2084980 0.73688024 9.495865 220-229
GACCG 2687075 0.73487526 9.604424 210-219
AACGC 2212470 0.7319141 14.828535 180-189
GGGAT 2233225 0.731898 13.226288 100-109
TTGGA 1711020 0.72466004 6.4803877 60-69
TCAAC 1691835 0.72327244 7.6223254 110-119
CATCC 1968860 0.7226005 6.614731 110-119
ATAAA 1240970 0.71982366 7.9676094 130-139
ACGCC 2533935 0.719644 13.172705 180-189
ATGCC 2035525 0.71940166 8.468861 50-59
TGCCA 2018480 0.7133776 7.744474 60-69
ATCTT 1254045 0.7127578 12.606709 3
TCCGG 2428815 0.709644 12.077652 70-79
GATTA 1384775 0.7094264 5.640064 50-59
CAACG 2140465 0.70809394 15.10202 180-189
TTTAC 1238760 0.7040703 13.134044 45-49
CGCCG 2992560 0.7026131 11.087932 220-229
TATTG 1273395 0.6969532 6.4158573 240-247
GGGCA 2644835 0.6965366 13.518294 150-159
GACCA 2090685 0.691626 6.0652757 170-179
TATAG 1345210 0.6891572 6.3788147 120-129
GAAAT 1434550 0.6879099 7.351436 110-119
AATAC 1380075 0.6872378 11.617478 130-139
CACAA 1709540 0.6840863 8.303199 130-139
GGATA 1720875 0.6822073 15.230126 35-39
CAAAC 1692750 0.6773677 13.792198 130-139
AAATA 1148550 0.6662155 12.944241 130-139
GCCGC 2832705 0.66508126 12.20585 220-229
CACGC 2336100 0.6634584 12.737079 190-199
TTTCT 1088820 0.66114575 9.855849 4
AGGCA 2035765 0.64851785 6.6058517 80-89
CCCGT 2127155 0.645407 9.997762 140-149
CAGTC 1821750 0.64384866 5.40686 210-219
TACTT 1128365 0.6413254 12.224828 1
GCGAC 2330285 0.63729846 7.0368404 70-79
ACTTC 1393220 0.6363204 30.462622 4
AATAA 1094115 0.6346405 7.5392623 130-139
GACGA 1982595 0.6315799 9.741329 220-229
CCGGC 2664185 0.625515 9.751107 70-79
AGATG 1569825 0.6223264 5.8862453 120-129
TCCAC 1691460 0.62079066 10.175923 180-189
TCAAT 1162435 0.61842245 8.5067425 110-119
TAGCT 1402485 0.61683065 12.855592 50-59
AAGCA 1591125 0.6131228 5.9702654 120-129
CACCG 2115805 0.60089415 9.029731 45-49
CCCTA 1622720 0.5955621 11.711927 110-119
CACGA 1776900 0.58782184 8.784941 180-189
GGCCC 2502175 0.58747727 10.168573 70-79
AGTAA 1219215 0.58465034 5.001623 50-59
ACCTA 1365380 0.58371043 7.2634764 130-139
TTAGC 1325955 0.5831718 10.168359 45-49
AATGG 1470690 0.58302635 6.633416 120-129
CCGTA 1636085 0.5782303 6.749768 230-239
CTTTG 1223105 0.5747034 7.1139503 45-49
ACCCG 2018265 0.57319254 8.810508 140-149
ACGAC 1725070 0.5706758 9.07212 220-229
TGATT 1041795 0.570194 6.090648 50-59
CGGTT 1554330 0.56514776 11.573948 160-169
CGGCA 2043340 0.5588232 11.256385 70-79
GGGGA 2198305 0.5575 8.621732 4
GTTCC 1470890 0.5553764 6.7210317 140-149
GGTGA 1690165 0.55392015 6.2323494 120-129
TAATA 893550 0.55372727 8.171325 130-139
TCAGG 1625020 0.5530511 5.4050283 150-159
ATTAA 876340 0.54306245 6.3752084 110-119
CTGCG 1858395 0.5429803 11.879495 25-29
TTCAA 1019800 0.5425398 5.3756847 200-209
ATTTC 949440 0.5396304 5.9906006 240-247
ACCCC 1803525 0.53190356 9.386528 140-149
GAAGA 1427815 0.529818 6.0868917 15-19
CCTAT 1159165 0.5294213 5.0188985 110-119
GCTAC 1488745 0.52615696 7.9579635 50-59
CCACG 1848810 0.52506685 7.663724 180-189
AAATC 1044760 0.5202606 7.9290147 110-119
GTCCT 1374415 0.51894957 6.343086 45-49
GGAGG 2039740 0.51728725 5.615949 6
AAGAT 1076745 0.5163317 5.50915 15-19
CTGTT 1098815 0.5163029 5.8618913 240-247
TATGT 929930 0.50896823 6.75113 140-149
GGGTA 1544400 0.5061484 5.440243 100-109
ACATT 931820 0.4957339 6.1756725 110-119
AGTTT 902255 0.49382114 5.001405 140-149
GGGTT 1390820 0.48696896 5.800565 100-109
TGACG 1420185 0.48333853 8.615 220-229
CGCGG 2128420 0.48121846 6.5285873 220-229
GTAAC 1163615 0.47903225 6.8564854 120-129
AGTAT 927665 0.47524694 5.2184825 110-119
TATCC 1027485 0.4692796 5.3265095 110-119
CCGGT 1589415 0.46439055 8.950722 160-169
TGGCC 1588960 0.46425757 14.647437 2
TAAAT 748625 0.46391818 8.062888 130-139
AACAT 927095 0.46166676 5.9795837 110-119
CCATT 1007595 0.4601953 5.048396 110-119
CATAA 912755 0.45452583 6.174152 110-119
CAATC 1054885 0.45097142 6.361159 110-119
GGTTT 993365 0.4494696 5.427692 100-109
ATGCT 1016020 0.44685844 6.9821677 50-59
CCGCG 1889835 0.44370797 6.735654 220-229
TGCTA 986115 0.43370584 6.195307 50-59
TGCGC 1458690 0.4261957 8.870962 25-29
TTGGT 938245 0.42452934 5.1697354 120-129
ATTCT 746090 0.42405292 6.9144936 2
GCCTT 1112450 0.42003724 9.141734 1
GCATG 1227020 0.41759777 8.421484 60-69
GGTCA 1224050 0.41658694 5.2791176 160-169
GCGCT 1411380 0.41237283 8.12262 25-29
CACTT 882220 0.4029332 7.209685 3
CTAAC 928735 0.39704132 5.4764576 110-119
ACGAG 1242800 0.39590913 7.7059646 180-189
ATGGC 1145400 0.38981965 6.7193933 1
CCGGG 1713105 0.3873191 5.1297545 80-89
CCTAA 905645 0.3871702 7.5379634 110-119
TACCT 844745 0.38581738 5.2048593 2
CATGC 1085350 0.38358784 6.9190574 60-69
GGTAA 965050 0.38257518 7.19263 120-129
AGCTT 860170 0.3783136 7.550665 1
TTTGA 686015 0.37546897 6.020942 45-49
GAGGC 1387715 0.36546487 5.7217193 35-39
TTCTT 592770 0.3599377 19.853811 1
CGGTG 1235545 0.34762916 5.154583 8
TGGGC 1219790 0.34319636 9.449309 1
ATGTG 786590 0.3331407 5.124412 140-149
CGAGC 1217535 0.3329778 6.488482 180-189
TGCTG 914570 0.33253378 13.845712 1
AACCT 729275 0.31177065 5.7426524 2
CTCTT 627720 0.3062911 9.765675 1
GCGAG 1144005 0.30128208 5.4061456 240-247
GCTTT 630350 0.2961841 5.007846 45-49
CGCTT 779510 0.29432625 5.805001 1
AACTT 542795 0.28877023 10.773041 3
GACTT 653690 0.2875011 6.8856764 1
CTCGG 963525 0.2815199 5.6195865 6
TCACA 655625 0.2802847 5.5296154 5
TTCCT 574250 0.2802008 6.6614203 4
ATTGT 504815 0.27629477 5.2525725 240-247
GAGGT 802330 0.26294875 7.0932508 7
CCCTT 664320 0.26047918 5.3573904 3
TAACT 482865 0.2568871 7.181194 2
GTCTT 536640 0.25215235 11.399523 1
TTGGC 680880 0.24756505 7.4319577 1
TGTAA 477190 0.24446659 7.5594716 25-29
AATCT 456000 0.24259476 7.1897364 2
CGGGG 1105555 0.24070041 8.5236 3
CCTCG 756820 0.22962919 6.0627337 5
TGCTT 451155 0.2119853 11.137752 1
TCGGT 537480 0.19542544 6.696761 7
TGCCT 515395 0.19460207 5.1621895 4
TCGCA 443805 0.15685096 5.6467195 7
TAGGC 455660 0.15507703 7.3079653 1
TTGCT 251855 0.11833972 5.571389 4
Produced by FastQC (version 0.10.1)