FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_7_SL248353.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_7_SL248353.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6181044
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 7016110 8.555206 59.552372 110-119
ATACA 6449730 7.4132805 36.36242 110-119
CACAG 9435830 7.36791 40.825264 110-119
TCCCT 10856295 6.8674135 101.67061 30-34
CAGTA 7770305 6.4367623 38.092514 110-119
AGTAA 6112825 6.3020062 46.254414 110-119
GGAGA 9744715 6.121662 94.19201 35-39
GGTGA 10889760 5.831446 100.66782 45-49
GTAAT 6632765 5.8289347 38.476665 110-119
CCCTG 10645180 5.6933436 66.632675 30-34
AATAC 4702635 5.4051795 38.270973 110-119
AGACG 7652550 5.359674 101.39911 40-44
GTAGT 8430685 5.339714 32.04739 80-89
ACAGT 6232565 5.16293 34.650448 110-119
GTGAC 8398490 5.0140786 84.079414 45-49
TAGTA 5457710 4.7962856 41.361805 80-89
GAGAC 6805890 4.766692 140.82825 40-44
CCCCA 6802105 4.7580824 100.67534 60-69
CTCAG 6970410 4.639597 34.057045 140-149
ACTCC 6089740 4.519107 114.182396 30-34
GGCCC 9781185 4.422915 57.746662 60-69
ACCAG 5613125 4.382974 88.41548 45-49
GCACA 5549065 4.332953 33.44892 110-119
GTGTC 8473185 4.312155 23.12632 120-129
AGTAG 5762850 4.2818823 33.380024 80-89
TGACC 6397570 4.2583065 144.33429 45-49
CGTGT 8360450 4.2547812 22.603922 120-129
CACTC 5588895 4.147437 94.01962 25-29
CTCCC 6788170 4.047614 65.69623 30-34
CCCAG 6340000 3.977827 86.25411 60-69
CCCTT 6055295 3.8304243 41.463806 20-24
ATGCA 4623155 3.8297272 91.46177 25-29
GACCA 4872365 3.8045561 101.94116 45-49
TGAGG 7034985 3.767221 68.58793 35-39
AGGAG 5871940 3.688772 94.33243 35-39
TACAC 3983720 3.6791725 26.474224 110-119
GACGG 7266645 3.6679773 108.367836 40-44
ATGGT 5781855 3.6620336 24.893785 190-199
ACGGT 6089240 3.6354067 96.50595 40-44
CCACT 4882125 3.6229534 26.78089 240-247
TGTCC 6332280 3.592855 19.992233 120-129
AGATA 3480135 3.5878391 18.703032 150-159
CTGAG 6000620 3.5824988 103.360054 35-39
CAGGG 6967890 3.5171752 33.350758 50-59
TGGTG 7694690 3.5124228 18.89237 25-29
GAGGA 5535770 3.477589 123.92427 35-39
GCCCC 6835160 3.4458616 72.506546 60-69
CTTGG 6751245 3.4358287 23.808432 20-24
TGCAC 5086155 3.3854115 110.33552 25-29
AGGCT 5670475 3.385395 29.176498 30-34
CCGTG 7033870 3.3742461 22.721514 120-129
CAGAT 4023465 3.3329563 17.304634 150-159
GGGTT 7181385 3.2781124 39.528706 50-59
GGATG 6114605 3.2743592 67.362816 20-24
TCTTG 5434935 3.2714417 17.475967 170-179
CTCTC 5121685 3.2398465 13.418377 100-109
CCTGA 4858765 3.2340581 80.77748 30-34
TCAGG 5368950 3.2053783 20.376 140-149
CTTCA 4052215 3.1901534 15.96564 200-209
CCAGG 5557360 3.1274707 37.774742 50-59
GCACT 4681085 3.1157916 85.52272 25-29
TCGCA 4669345 3.1079774 23.269712 100-109
TGTCT 5105990 3.0734403 19.075523 180-189
TCTCA 3901560 3.0715485 21.034546 130-139
CCTGG 6386835 3.0638545 27.844767 50-59
ACAGA 3135910 3.0474408 13.983173 140-149
CGCAC 4819365 3.0237544 23.808313 100-109
GCCCT 5536005 2.960812 22.210964 20-24
GGTTG 6436170 2.9379418 70.510025 15-19
CGGAT 4870470 2.907775 76.30211 20-24
GACTC 4288225 2.8542988 18.934282 190-199
GATGG 5325210 2.8516397 22.880955 15-19
TGGCC 5920705 2.8402452 42.11488 60-69
TTCCC 4482590 2.8355713 46.640102 50-59
ACCAC 3244875 2.8248417 18.190302 230-239
GATGC 4684915 2.7969947 91.61437 25-29
TCTCT 4166480 2.7960575 14.482353 180-189
GGTTC 5478365 2.7880373 44.555275 50-59
AGGGT 5156855 2.7614863 47.084118 50-59
CGGTG 6389255 2.7491639 68.266594 40-44
GCCGT 5676875 2.7232766 21.447458 120-129
GTTGG 5938700 2.7108598 50.781414 15-19
CGGCC 5950665 2.6908078 12.380861 120-129
TTCTT 3769365 2.6835532 19.876343 170-179
TCCAT 3407380 2.6824994 20.696917 70-79
TCAAA 2314000 2.6596975 54.41826 70-79
TCACA 2861185 2.6424534 11.272038 140-149
AGATG 3508655 2.6069822 16.42554 180-189
TGGAG 4840005 2.5918133 20.06684 25-29
GGCTG 5998870 2.5811892 15.211533 30-34
AGTCA 3088715 2.5586283 28.645494 70-79
CCAGT 3833585 2.5516844 33.606613 60-69
GTTCC 4467315 2.5346975 50.374683 50-59
AGGGA 4031175 2.5323973 8.87709 150-159
TCCTC 3904900 2.4701395 16.382572 130-139
ACTCT 3099900 2.4404323 13.352257 200-209
TGTTC 4046690 2.4358175 12.4142 140-149
GCGGA 4760370 2.4028873 83.47726 20-24
GAGAT 3210300 2.3853004 15.942263 180-189
AAAAA 1411560 2.3687432 8.6406355 130-139
ACAGC 3027605 2.3640866 10.781318 120-129
TGTAG 3731910 2.363667 7.8536057 210-219
AATAA 1649795 2.3599694 10.172011 110-119
GGCGG 6461275 2.3505604 40.344418 20-24
CTGGA 3887220 2.3207536 14.045176 180-189
GTTCT 3839135 2.3108842 16.825653 160-169
TACCA 2497420 2.3064973 15.441316 230-239
CACGG 4080530 2.296367 9.524051 120-129
CAGCT 3430420 2.2833326 11.890158 150-159
TTGGG 4965140 2.2664554 76.56604 15-19
CAGAG 3186620 2.231837 9.10623 140-149
CATTT 2656730 2.2188694 17.115362 150-159
TCATT 2640410 2.205239 15.927779 150-159
CTGGC 4591705 2.2027051 32.49226 60-69
TTCAT 2627560 2.194507 15.330958 140-149
GTGAA 2935305 2.180975 17.206335 190-199
TCAGC 3254560 2.166278 13.912014 140-149
TTGTC 3587415 2.1593668 12.771301 170-179
TTCAC 2737145 2.154849 17.538666 200-209
CTACT 2731295 2.1502438 7.3922834 230-239
ACTAC 2321065 2.1436243 12.01744 230-239
GATAC 2574880 2.132978 14.020544 150-159
ATGTA 2423375 2.1296842 9.2767 70-79
ATTTG 2788770 2.089128 15.055666 150-159
CACTA 2259650 2.0869045 14.368511 240-247
CCAGA 2670495 2.0852394 31.473066 60-69
CTCGC 3896295 2.083849 16.020636 100-109
TCTGC 3670295 2.0824785 11.568914 210-219
GAACT 2485040 2.0585566 10.475323 160-169
CTCTG 3626005 2.057349 14.003782 180-189
GAATC 2476230 2.0512583 14.007491 190-199
GCTGT 4019645 2.0456686 9.750616 140-149
TCTCG 3589105 2.0364125 15.7326565 100-109
ACACA 1868450 2.0243478 13.347619 110-119
ATCTC 2566260 2.0203178 7.9406185 100-109
TTGGA 3189085 2.0198598 14.918344 170-179
GTCCT 3553580 2.016256 13.907051 130-139
TGGGG 5222840 2.0156968 50.534515 15-19
GTCTC 3544175 2.0109198 11.406516 180-189
TGAAT 2287270 2.010074 13.522976 190-199
AGCTC 3013275 2.0056753 11.913146 140-149
TACAG 2415825 2.00122 13.112618 160-169
GATAT 2258420 1.9847202 8.00268 180-189
CAGGC 3517920 1.9797513 14.85056 140-149
GTTCA 2796170 1.9744709 14.356406 140-149
AATCG 2379615 1.9712244 12.964868 190-199
TTTGC 3267570 1.966843 11.549481 150-159
ACACG 2510005 1.9599218 13.359653 120-129
ACGGC 3478185 1.95739 14.113129 120-129
ATATA 1600325 1.9513819 10.08007 110-119
TAGTC 2760530 1.9493037 23.6934 70-79
TGGAA 2618205 1.945365 8.556526 170-179
TTGCA 2736855 1.9325864 13.564795 150-159
AATAT 1562630 1.9054178 12.612885 110-119
AACCA 1754315 1.9006898 14.361128 70-79
GACGT 3182175 1.8998266 9.938917 60-69
CCTTC 2999130 1.8971727 13.1553545 200-209
ATATG 2158660 1.8970503 8.234274 180-189
CCTTG 3342745 1.8966309 27.00524 20-24
GAGTC 3159200 1.8861102 11.678292 210-219
TACTA 1914755 1.87603 9.935789 240-247
CAGCC 2987855 1.8746327 9.898766 120-129
CATAG 2244020 1.8589005 8.115799 210-219
TCGGC 3861930 1.8526219 7.4302373 130-139
GTCAA 2229730 1.8470628 36.760216 70-79
GTTGT 3329920 1.7978219 6.8105116 210-219
CACCA 2058605 1.7921287 12.629111 230-239
GGGGC 4913160 1.7873685 45.985157 15-19
AGTAT 2030255 1.7842067 11.412538 220-229
CTGTT 2958340 1.7807088 12.123995 140-149
CGTAG 2960435 1.7674432 9.330528 210-219
AGAAC 1802255 1.7514104 11.018738 160-169
CTCCA 2346490 1.7412958 7.728761 150-159
GTCCC 3250935 1.738692 23.399672 50-59
GGGCG 4778070 1.7382239 56.272007 20-24
ATTGT 2283950 1.7109563 8.425444 50-59
GTCAC 2562150 1.7054007 8.367005 130-139
GCAGA 2432190 1.7034512 13.723988 150-159
GGTCA 2847125 1.6997947 7.432898 130-139
TTGGT 3142725 1.6967554 28.479479 25-29
TGACT 2393665 1.690248 11.426236 190-199
TCCCA 2262370 1.6788716 10.960121 240-247
TGCAG 2810280 1.6777974 12.754523 150-159
AGTCT 2367430 1.6717225 12.321924 210-219
TCAGA 2014660 1.6689032 10.242304 130-139
CAAAA 1237400 1.668481 21.878603 70-79
ATAGT 1894495 1.6648997 7.1182933 220-229
CTGTG 3219860 1.638644 5.3637414 210-219
CAGAC 2083005 1.6265016 18.986092 60-69
AACTG 1960320 1.623889 10.284636 160-169
GAGAA 1859585 1.6208982 10.253222 160-169
TGGTT 2998145 1.6186967 17.53563 10-14
TTCAG 2276005 1.6071644 9.146389 150-159
TGTGC 3155295 1.6057857 7.8700175 220-229
AATAG 1544985 1.5927995 8.960353 240-247
GCTCC 2967830 1.5872796 6.2213078 150-159
TGCTC 2791085 1.583626 5.418593 150-159
ATAGA 1532005 1.5794182 9.799546 240-247
GTCTG 3100365 1.5778309 8.531671 210-219
ACTGA 1903425 1.5767585 8.403064 180-189
TTGTA 2087925 1.5641098 8.668756 210-219
CTGGT 3070525 1.5626447 6.761796 160-169
TCTGG 3066925 1.5608127 11.370363 180-189
ACCAT 1685840 1.5569609 12.887042 45-49
TAGTT 2071545 1.551839 9.398011 220-229
GTGCT 3045080 1.5496953 7.5472345 220-229
GGCCG 3819055 1.5489632 12.707665 120-129
GCTCA 2323605 1.546622 10.7960415 140-149
GGACG 3062030 1.5456179 6.9835577 170-179
GCTGA 2584960 1.5432765 24.72662 30-34
CCCAC 2181510 1.5259695 10.340364 240-247
GAACC 1952870 1.5248865 11.826521 70-79
CATGT 2137745 1.5095344 9.287888 70-79
CCACC 2153835 1.5066108 9.094448 230-239
CAAAG 1500580 1.4582462 26.132881 70-79
GTGGA 2722755 1.4580302 25.371742 25-29
CCTCG 2725150 1.4574873 9.195949 130-139
AGATC 1753530 1.4525884 6.9854016 130-139
ACCGT 2180080 1.4510899 22.346035 45-49
GCTCT 2530860 1.4359777 9.405222 130-139
CGACT 2150740 1.4315608 8.382622 190-199
TCGAC 2110505 1.4047798 9.036096 190-199
CACTG 2101155 1.3985565 6.993595 230-239
TGATA 1589940 1.3972539 7.3864717 180-189
CTCTT 2082065 1.3972403 7.8874793 200-209
CCTCA 1872545 1.3895882 7.303541 130-139
TGGAC 2315865 1.382621 9.975237 170-179
CCATG 2063205 1.3732965 9.388412 70-79
GTCCA 2062255 1.372664 19.387308 70-79
GTAGG 2552535 1.3668776 5.2116446 160-169
GAGGG 2997705 1.3572181 7.890421 200-209
GGGAG 2993590 1.3553549 6.414917 150-159
TATAC 1376360 1.3485239 6.4795675 110-119
GCGTA 2254370 1.3459072 9.822877 210-219
GGAGG 2968800 1.3441312 19.12072 30-34
ACTTC 1699975 1.338325 6.600759 220-229
AATTG 1521855 1.3374201 8.482337 170-179
CCATC 1799095 1.335082 10.59072 240-247
CTGCT 2348350 1.3324238 5.3789325 90-99
GTGGT 2901880 1.3246316 12.870408 50-59
CTCGA 1980795 1.3184433 8.036488 190-199
CTTGA 1865320 1.317166 8.093814 200-209
TATGG 2077150 1.3155973 6.082585 180-189
TGGTC 2576385 1.3111682 14.485906 50-59
TCCAC 1755370 1.3026344 9.456393 240-247
CTGAT 1842160 1.3008118 7.0746717 180-189
ACGGA 1851395 1.2966754 8.792325 200-209
TTGGC 2528250 1.2866714 9.316058 60-69
GTGTT 2355370 1.2716628 7.1827044 160-169
ATCGG 2113740 1.2619481 7.704361 190-199
TGCCC 2354725 1.2593735 8.233117 60-69
AGCCG 2235455 1.258029 8.794601 120-129
ACGTG 2106115 1.2573959 8.997163 170-179
GAATT 1418435 1.2465338 9.420115 170-179
ATAAA 863540 1.2352614 5.285115 7
ACTAT 1258915 1.2334541 6.2253876 230-239
GGAGT 2289530 1.226039 6.559249 210-219
ACTCG 1832350 1.2196363 8.240009 190-199
GGCTC 2533795 1.2154968 7.8481846 130-139
GATCT 1720650 1.2150093 5.5290904 130-139
AAGTA 1177070 1.2134982 14.956855 70-79
TTGAG 1887580 1.19553 7.8085036 200-209
GGAAT 1604385 1.1920817 7.924963 170-179
ATAAC 1035445 1.1901342 6.9178085 80-89
ATACC 1286505 1.1881543 8.573361 70-79
CCATA 1282955 1.1848758 11.854167 70-79
GCATA 1430310 1.1848396 7.6917734 210-219
ACTGG 1984055 1.1845233 6.8361235 160-169
ATGGG 2179880 1.1673216 36.055943 15-19
ACTGT 1650030 1.1651422 5.376434 230-239
AAAAG 953370 1.1530302 15.025074 70-79
TTCCA 1454420 1.145009 7.12327 230-239
TCTGT 1901600 1.144627 6.4750013 210-219
TATCA 1167520 1.1439074 7.3438888 70-79
AGAGC 1630315 1.1418359 6.733188 140-149
GAAGA 1307835 1.139968 19.68704 35-39
AGCTT 1613945 1.1396614 5.9635005 150-159
AAAGT 1095775 1.1296875 19.052551 70-79
AGAGA 1292640 1.1267234 14.447094 35-39
CAATA 976200 1.1220382 6.5563626 100-109
AGTTC 1588725 1.1218525 5.355774 160-169
ATCCA 1199475 1.1077776 15.1500845 240-247
TCACG 1660325 1.1051342 8.810459 200-209
TCTAC 1396530 1.0994344 6.994609 180-189
CTGAA 1322240 1.0953166 15.843459 35-39
ATATC 1114145 1.091612 7.836217 70-79
GTCTA 1544840 1.0908641 7.184721 180-189
AGGTT 1719040 1.0887824 22.588593 10-14
AACTT 1105375 1.0830193 6.0566144 200-209
CTGCC 1985800 1.062062 5.7861776 200-209
TACTG 1500875 1.0598189 6.161583 180-189
CTCGG 2200480 1.0556012 7.8131156 130-139
CGGCT 2200120 1.0554284 7.3950477 130-139
GCTTC 1839455 1.0436833 5.294639 150-159
TATGT 1392965 1.0435002 7.1444654 220-229
GAGCT 1746995 1.0429934 6.0515084 140-149
GTCCG 2171530 1.0417134 5.0206513 130-139
CATGG 1728030 1.0316709 5.33786 190-199
CTTCT 1530370 1.0270066 5.4552903 210-219
TAGAT 1166425 1.0250651 7.4740086 240-247
CTGCG 2133470 1.0234555 6.479381 210-219
TCCCC 1712965 1.0213977 5.313537 80-89
CGTGG 2371820 1.020545 8.8399315 50-59
GCGGT 2366095 1.0180817 5.6607447 130-139
ACGTC 1521310 1.0126039 13.350931 70-79
AAAAT 700430 1.0019386 5.085056 8
CTACC 1345960 0.9988172 7.8875394 230-239
AAGAG 1140380 0.9940067 14.662806 35-39
TGCGT 1930745 0.9825904 7.798434 210-219
TATAA 802765 0.9788644 6.213507 240-247
GGTGG 2529880 0.9763789 25.266163 25-29
GGGGT 2518080 0.97182477 7.2538786 10-14
CCATT 1220745 0.9610457 6.3505073 50-59
CGAAC 1227240 0.95828277 12.435754 70-79
ACAAT 833320 0.9578129 7.520726 100-109
TAGGC 1600810 0.9557179 5.8129907 160-169
GAGGC 1888730 0.9533724 16.146576 30-34
ACCCA 1092005 0.9506503 12.272965 240-247
CCCAA 1089955 0.94886565 9.5004015 240-247
TGAAG 1264800 0.939765 10.552293 35-39
GAGTT 1476100 0.9349124 5.12189 160-169
CGGAG 1834140 0.9258171 6.1723175 210-219
TGCAT 1292225 0.912484 5.9247336 210-219
CAGCG 1621255 0.9123806 5.766861 160-169
TGTAA 1035510 0.91001576 6.333134 230-239
TTCTG 1504380 0.905529 5.387807 210-219
GGGCC 2230515 0.90467024 19.135439 15-19
ACTAA 786055 0.90348685 11.607066 240-247
ATCAA 777965 0.89418817 13.925704 70-79
CGTCC 1661795 0.888775 10.9025955 70-79
TCGAA 1066120 0.88315207 16.324472 70-79
GACCG 1565530 0.8810207 20.471502 45-49
CATCA 948430 0.8759245 5.355595 230-239
CCAAT 936760 0.86514664 10.316471 240-247
TGGGC 2001210 0.8610792 20.76636 15-19
CCCCC 1528710 0.8592236 5.0814357 80-89
GGTCC 1776775 0.85234386 14.614636 50-59
CGGTC 1755655 0.8422123 5.361408 130-139
TGAAC 1014340 0.84025854 6.0078726 190-199
CCAGC 1327515 0.83290625 5.457791 80-89
GTATG 1305605 0.8269265 5.0502105 220-229
CTTCC 1266905 0.80141157 5.0104847 230-239
AGCGT 1333060 0.7958654 5.91105 160-169
AAAGA 652980 0.78973085 5.6273427 8
TGAGA 1038505 0.77162457 5.4843993 240-247
CATAC 831275 0.7677257 10.286984 70-79
TAACC 828690 0.76533836 5.5511303 80-89
AACCG 975120 0.76141644 5.2465477 190-199
AATCC 804930 0.7433947 9.207493 240-247
CCTTT 1104945 0.74151075 5.5655875 50-59
AAAGG 843155 0.73493195 8.607071 10-14
ATGAT 833195 0.7322194 6.6273103 240-247
AGCCA 924815 0.7221361 5.537305 80-89
CTAAT 736855 0.7219525 8.890307 240-247
GTCGA 1190830 0.71095103 11.32867 70-79
GTAGC 1186650 0.7084555 5.3574243 80-89
AAAGC 726715 0.7062131 10.197582 70-79
CATTG 985125 0.6956303 5.8196487 50-59
GGAAG 1105750 0.6946358 6.862384 30-34
AAGGT 927790 0.6893617 9.6079 10-14
CGATG 1146070 0.68422836 15.779087 15-19
TAGGT 1073010 0.67960864 5.261463 10-14
AGGAT 911045 0.6769199 6.739936 10-14
GAGGT 1227905 0.65754086 6.656417 10-14
CCGTA 978550 0.6513357 5.476789 80-89
TATGA 738240 0.6487721 5.5327096 240-247
AGAAG 740345 0.64531815 5.4352717 9
CAATC 690620 0.63782346 8.88009 240-247
ATAGG 833965 0.6196483 5.1934595 10-14
CATCC 814855 0.6046919 7.409219 240-247
TAAAA 414540 0.59298384 5.6787243 8
GAAAA 480850 0.5815524 5.8738284 8
AAGAA 474425 0.57378185 5.204582 8
AATGG 771485 0.57322466 6.454754 10-14
GGGTC 1321020 0.56840754 5.3674927 70-79
AAATG 541185 0.5579338 5.2674284 9
AAGCA 563515 0.54761726 6.955319 70-79
GAAGG 864055 0.5428022 5.4409122 10-14
TTGCC 902700 0.51218045 5.7763605 60-69
ACGAT 613700 0.50837654 7.0621233 10-14
AAGGG 806435 0.5066051 6.030356 10-14
TGATC 710730 0.5018705 5.193201 240-247
ATAAG 468345 0.48283955 5.7398033 9
GATCC 720340 0.47946775 5.2093663 240-247
GGTCG 1113920 0.47929668 5.447356 70-79
GAAGC 644735 0.4515579 5.4691586 30-34
TACCG 677505 0.45095623 5.4761143 70-79
CGGTT 860930 0.43814257 7.8408985 10-14
TAAGG 588025 0.43691126 5.802177 10-14
AAGCT 521175 0.43173075 9.194543 30-34
TAAAG 386195 0.39814714 5.4584155 9
Produced by FastQC (version 0.10.1)