FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_1_SL248338.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_1_SL248338.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6429203
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30910 0.48077498875054964 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24509 0.3812136589869693 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24347 0.3786939065386487 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24154 0.3756919792391063 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23455 0.3648197140454268 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22753 0.353900786769371 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 22213 0.3455016119416357 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21323 0.3316585275033313 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 20109 0.3127759381683857 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19991 0.31094056292825095 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18753 0.291684676934295 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18474 0.28734510327329843 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18287 0.28443650013850863 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18261 0.28403209542458063 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18205 0.28316106988688955 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17949 0.27917923885744467 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17614 0.27396863965875706 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17435 0.27118446874363744 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17341 0.26972239016251315 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17256 0.26840029782851776 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16995 0.26434069666177906 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16825 0.26169651199378835 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16603 0.25824351789794164 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16512 0.25682810139919365 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16161 0.25136863776116575 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15560 0.2420206672584456 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15205 0.23649898751058254 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15060 0.23424365352906107 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 14760 0.22957744529143037 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14677 0.22828646101235253 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14567 0.22657551799188796 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14567 0.22657551799188796 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14475 0.22514454746568122 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14398 0.22394688735135598 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13364 0.20786402295898884 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13274 0.20646416048769964 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13056 0.20307338250168802 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12992 0.20207792474432681 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12963 0.2016268579480225 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 12905 0.20072472435541389 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12870 0.20018033339435698 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 12587 0.19577854362352534 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 11995 0.1865705593679341 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 11908 0.1852173589790212 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11791 0.1833975377663452 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11644 0.18111109572990616 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11421 0.1776425476066007 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11037 0.1716698010624334 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 10953 0.1703632627558968 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10942 0.17019216845385035 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 10829 0.16843456335100945 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 10541 0.16395500344288397 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10423 0.16211962820274925 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 10370 0.16129526474743447 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10100 0.15709567733356686 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10033 0.15605355749382932 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 9934 0.1545137087754112 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9710 0.15102960662464693 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9287 0.14445025300958766 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 9166 0.14256821568707662 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9132 0.14203937875347847 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 8943 0.13909966756377112 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 8643 0.13443345932614043 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 8602 0.1337957442003309 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 7859 0.12223910179846555 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7666 0.11923717449892311 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7575 0.11782175800017514 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7510 0.11681074621535516 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 7457 0.11598638276004039 No Hit
AAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 6516 0.10135004292133878 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6489 0.10093008417995201 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 13282040 7.8004737 66.71158 35-39
TATTA 6664795 7.6305623 132.63731 240-247
TGGAT 10065525 7.349015 80.47775 35-39
TCACC 10379160 6.5988297 76.799545 160-169
TCTCC 9080870 6.1599407 85.69544 20-24
CTGTG 9672660 6.061031 87.977165 25-29
TCCCT 8826930 5.9876823 75.30627 15-19
AGTGG 10442175 5.894174 40.977924 100-109
TGTAT 6125590 5.7849736 62.834267 240-247
CTGAG 9812325 5.762728 50.676727 15-19
TTACT 5660125 5.5616503 116.12263 240-247
ATTAC 5593015 5.1508546 109.97023 240-247
CCTGA 8129005 4.967271 52.211994 15-19
TGGGT 8225770 4.9539647 46.503056 100-109
CTCTG 7380870 4.812075 75.994286 35-39
TTCAC 6084000 4.80873 77.9592 40-44
ACACG 8345490 4.7795596 51.37318 230-239
CTCCA 7425530 4.72098 42.20087 80-89
GACAC 8197045 4.6945443 47.89776 230-239
TGAGA 6668285 4.563127 96.5239 15-19
TACTG 5849390 4.4435244 81.38466 240-247
AAGGG 8379180 4.4329123 41.150642 90-99
ATTCA 4812475 4.4320207 116.09965 40-44
TCTGG 6861520 4.2995296 137.96107 35-39
GAAGG 8065790 4.267117 33.240883 90-99
AGCCT 6926775 4.232642 52.897293 30-34
CCCTG 8070905 4.232632 87.5773 10-14
TGGAG 7471755 4.217496 39.732513 90-99
ACCAT 5692145 4.2167006 70.264694 160-169
ACTAC 5659250 4.1923323 37.38866 130-139
CAGCC 8525520 4.1904902 57.279156 30-34
TCAGT 5504800 4.181754 96.55228 7
CCAGG 8844375 4.1781774 54.896744 80-89
TACTA 4518895 4.1616497 31.498299 130-139
GAGAC 7551600 4.1567206 92.6757 8
GTATT 4322615 4.082254 72.75855 240-247
CACCA 6847610 4.0803676 59.435753 160-169
TCCAG 6603610 4.0351706 41.54916 80-89
GGGTC 8201145 3.9729621 128.49022 8
ACTGT 5180135 3.93512 70.04258 240-247
CACCT 6142665 3.9053638 45.16821 20-24
CAAGA 5765995 3.8477113 46.569447 180-189
TGAAG 5569410 3.8111637 41.325478 150-159
CTGGG 7835775 3.7959616 135.39899 3
AGACT 5311740 3.7818944 70.852684 15-19
GACTC 6181430 3.7771955 52.499763 20-24
GATTC 4938610 3.7516444 60.182503 40-44
GCTGG 7550615 3.6578186 34.65441 90-99
GGACA 6606135 3.6362972 29.343914 220-229
ACTGG 6123560 3.5963354 46.155502 60-69
CCTGT 5407990 3.5258248 60.93341 25-29
ACTCT 4371465 3.45516 69.146736 20-24
TATCT 3515805 3.454637 51.201813 200-209
AGACA 5097640 3.4017107 40.205563 170-179
GGATT 4656285 3.3996348 57.51442 40-44
GAGTG 6008255 3.3914104 39.851166 100-109
AGCTG 5717410 3.3578062 38.363323 60-69
TCCTG 5044810 3.289044 63.204502 20-24
CTGCA 5291465 3.2333775 47.315434 25-29
GGTCC 6395190 3.2234251 143.21242 9
AGTAG 4707920 3.2216432 31.960524 50-59
CTCCT 4749025 3.2214658 92.98779 20-24
GTCCC 5996235 3.1446114 75.44164 10-14
GTCTC 4799605 3.1291783 35.296722 30-34
CCTCA 4911905 3.1228755 81.46688 5
CATCT 3945335 3.1183512 32.83287 170-179
AAGAA 4004365 3.1135366 40.50596 180-189
CCATC 4869670 3.096023 37.876774 45-49
GGAGT 5392940 3.044091 38.08595 90-99
GGCTG 6251785 3.0286143 35.985653 90-99
GAGCT 5073245 2.979491 31.61317 60-69
AGGGA 5599485 2.9623456 42.520485 80-89
GGAAG 5383515 2.8480897 31.93181 80-89
TGTGC 4544120 2.8474126 58.52831 25-29
CAGGG 6183780 2.8076925 37.979507 80-89
ATCTG 3686410 2.8004034 38.381393 200-209
GTGTA 3824800 2.7925532 38.033043 230-239
GAACA 4178145 2.7881217 36.63752 210-219
GTGAA 4065655 2.7821395 40.218143 150-159
GACAA 4156710 2.7738178 25.436777 180-189
ATGAA 3336870 2.7682416 28.586805 210-219
ACCTT 3476100 2.7474728 73.574104 40-44
CTATG 3584815 2.7232263 28.192413 50-59
ATCTC 3395775 2.683985 49.059868 170-179
AGAAC 4015635 2.679677 32.60605 190-199
GGGAA 5063690 2.6788895 35.2003 80-89
GAGTC 4526930 2.658643 29.407427 150-159
CACGG 5544255 2.6191654 45.8694 230-239
CCCTC 4750025 2.5918417 28.604551 20-24
CGCCA 5258540 2.5846946 40.375706 70-79
TGCAA 3618425 2.5762749 37.461952 200-209
ATCAG 3616330 2.574783 29.791666 45-49
CCAAG 4426990 2.535389 41.073654 180-189
AGGGC 5567205 2.527742 33.3637 150-159
CTACA 3411110 2.5269263 19.706112 190-199
CAGTG 4288290 2.5184908 74.62862 8
ACGGC 5312985 2.509911 50.7076 230-239
AGGCT 4266855 2.5059023 20.658163 25-29
CTGTC 3803635 2.4798398 52.29876 30-34
CTACT 3135335 2.478136 23.216389 50-59
TCTGT 3044530 2.4676387 26.52284 210-219
CACTG 4016960 2.4545844 21.080704 30-34
TATAT 2143155 2.4537106 25.014196 240-247
GTGGA 4336235 2.4476247 27.81859 100-109
TGAAC 3387000 2.411503 39.237232 210-219
GGGCT 4953075 2.3994672 43.923122 90-99
GCAGC 5072890 2.3964875 35.157948 30-34
CCGCC 5658950 2.387194 34.900032 70-79
GTAGT 3243855 2.368395 28.513384 120-129
CTTCA 2995440 2.3675647 52.566345 45-49
CAAAT 2731190 2.3574412 37.044285 200-209
GTATC 3097370 2.3529358 23.850416 200-209
CAGTA 3296070 2.346762 22.950466 45-49
ACAAC 3347280 2.3240445 21.229147 180-189
GTGGT 3856010 2.3222797 32.934692 120-129
CTCTC 3423435 2.3222616 98.63475 20-24
ACAGC 4041115 2.3143942 28.34116 210-219
GGCCG 5933830 2.31227 26.135479 230-239
TCCAA 3120060 2.3113184 45.020084 180-189
TATGC 3010485 2.286933 21.261484 50-59
TGGTG 3783975 2.2788966 36.637726 35-39
AAATG 2743525 2.276007 35.19494 200-209
AATGA 2726275 2.2616968 26.995304 210-219
CAGGC 4772650 2.2546508 33.57912 70-79
GCTCC 4283730 2.2465208 34.55625 80-89
TACAT 2416800 2.2257378 18.684814 190-199
GCCTC 4227060 2.2168012 71.26816 4
TATCA 2397740 2.2081847 19.209827 170-179
GTGGG 4658985 2.169237 30.593637 100-109
CCTTC 3192550 2.1656425 57.416756 45-49
GCCTG 4296160 2.1654322 31.028399 210-219
GCTAT 2845870 2.161882 34.88913 50-59
GGGGG 6005150 2.1616173 96.964005 5
GCTGT 3424215 2.1456635 20.198812 230-239
TCTAT 2173920 2.1360984 20.741348 110-119
ATATC 2266420 2.0872462 15.002184 170-179
AACAG 3127340 2.086908 35.37472 210-219
GCAGA 3777045 2.0790453 25.42677 140-149
CCTCT 3058515 2.0747209 49.776993 35-39
TGAGC 3527320 2.0715773 18.60628 200-209
CATAT 2248585 2.070821 15.699075 160-169
AACTA 2396230 2.0683184 37.919083 130-139
TGCAG 3518220 2.0662324 44.668 30-34
AGCAC 3601355 2.0625386 22.82834 130-139
CTCAC 3214985 2.044013 57.47088 20-24
TGTGA 2791750 2.0383053 20.299137 210-219
TGCAC 3315855 2.0261705 28.305435 25-29
CAGAG 3680525 2.0259168 33.607925 170-179
GGCTC 3981865 2.0070152 21.316036 40-44
AGAGT 2929325 2.0045455 32.296196 150-159
TAGTA 2255090 1.9960572 19.909029 120-129
TGGTA 2732970 1.9953891 32.36966 120-129
TCCGC 3786470 1.9857422 43.201176 70-79
ATGGA 2876250 1.9682263 12.790577 100-109
CTGTA 2587385 1.9655223 25.972223 190-199
CCGTG 3896520 1.9639981 20.942272 230-239
TTCAG 2563135 1.9471009 48.910408 45-49
CTATT 1960295 1.9261899 21.083164 110-119
TCCAT 2431530 1.9218559 28.95384 45-49
GGTCT 3066515 1.9215236 35.177643 15-19
CAGTT 2524875 1.9180362 27.018892 190-199
TGGGG 4106260 1.9118866 126.16597 4
CAGAC 3335020 1.9100053 27.47336 140-149
ACCAG 3331350 1.9079031 15.3833475 190-199
TCTCT 2262045 1.9075978 34.953613 35-39
GCACA 3328175 1.9060848 19.305376 130-139
GCCAG 4018860 1.8985527 36.070713 70-79
GGTGG 4067005 1.8936093 19.779825 40-44
GTCAC 3089805 1.8880415 38.99667 160-169
TGGGA 3333465 1.8816028 16.715775 100-109
ACCCT 2957920 1.8805768 45.182076 10-14
AGAGA 2927835 1.8778054 29.270603 170-179
TAGTG 2571310 1.8773583 28.650799 120-129
GGATC 3196020 1.8770065 19.370785 70-79
ACTAT 2033945 1.8731496 17.497978 130-139
ATACT 2031380 1.8707877 28.841648 130-139
GCTGA 3172800 1.8633691 28.036644 200-209
TGTCT 2294355 1.8596102 53.429695 30-34
CCAGA 3236615 1.8536472 35.24865 170-179
AGGAC 3354480 1.846448 21.396164 220-229
CCTGC 3500840 1.835949 49.954994 20-24
GATGG 3238715 1.8281201 20.799896 100-109
GCCGA 3868185 1.8273721 20.505291 220-229
TACAA 2111205 1.8222978 18.902729 130-139
AGTCA 2556295 1.8200511 30.749712 160-169
GAGGA 3434900 1.8171959 28.007608 220-229
AGTTC 2375925 1.8048855 23.963675 190-199
CACAG 3128895 1.7919548 15.901383 140-149
CATGA 2510925 1.7877482 25.158909 60-69
TTCCA 2246590 1.775681 23.245298 180-189
GCAAA 2653035 1.7703992 34.061672 200-209
TCAGC 2885925 1.7634596 12.023098 45-49
TGACC 2868315 1.7526989 14.830661 210-219
AGCTA 2449360 1.7439146 27.082943 50-59
ACCTG 2844590 1.7382016 32.206932 25-29
CAAGG 3126445 1.7209278 17.266708 80-89
GGAGC 3783430 1.7178342 12.993829 200-209
ATTGG 2351545 1.716904 21.988184 100-109
GTGCA 2901775 1.7041978 52.01239 25-29
AACAC 2447260 1.6991532 23.182686 190-199
CTGAA 2384625 1.6978241 25.201128 200-209
AGGGG 3852625 1.6812335 34.794586 90-99
TTCTG 2072550 1.6798338 42.276634 30-34
CTCAG 2731890 1.6693357 77.92652 6
GGATG 2946510 1.6631826 22.54232 100-109
GGGGC 4429570 1.6589785 30.02584 90-99
ATGCA 2317020 1.6496902 15.936394 60-69
AGTTA 1852865 1.6400338 17.575474 50-59
TCTGC 2503135 1.6319586 34.636948 200-209
CCTGG 3224215 1.6251302 34.958916 2
GCCGT 3213185 1.6195706 30.386057 230-239
CGTCC 3051330 1.600212 20.064903 180-189
CGATT 2102680 1.5973133 35.58848 160-169
GTTCT 1966280 1.5937004 25.201483 190-199
CTCCC 2909795 1.587724 14.466683 190-199
GGGCC 4067535 1.58502 28.711664 2
TTAGT 1665255 1.5726577 27.683533 110-119
GTTAC 2067635 1.5706913 17.112066 50-59
TTCTC 1853935 1.5634357 23.688547 190-199
CAGCT 2536400 1.5498804 12.989436 50-59
ACAAT 1793995 1.5484962 23.98621 180-189
GCACT 2526510 1.543837 27.357122 25-29
TCTCA 1952860 1.5435202 27.370281 100-109
AGTGA 2227320 1.5241615 86.5693 9
CGGCC 3754040 1.5220416 32.235107 230-239
GAGCA 2743885 1.5103506 7.7801166 200-209
CCATG 2458850 1.5024931 15.881811 160-169
GGCTT 2393105 1.4995549 21.770609 25-29
GTCCG 2969015 1.4964994 34.644745 70-79
TCTGA 1956560 1.4863125 21.151348 220-229
CATCA 2004165 1.4846711 34.11076 45-49
CTCAA 1983155 1.4691073 20.62712 150-159
CTTTA 1492580 1.4666122 42.379467 45-49
TAGCA 2042390 1.454157 12.340579 130-139
GTGAC 2455420 1.4420558 16.09221 210-219
AAGCT 2017230 1.4362433 18.419985 200-209
CCATA 1936620 1.4346343 16.154175 160-169
TACGC 2345455 1.4332025 25.804617 140-149
ACTCC 2243725 1.4265083 17.368816 140-149
CAACT 1924235 1.4254596 18.636742 130-139
AACCC 2377180 1.4165189 21.971634 140-149
CTGGT 2257110 1.4143384 33.34254 35-39
CAACC 2369370 1.4118651 16.845442 140-149
GGGGT 3016075 1.4042933 120.75176 7
GTCCA 2296650 1.40338 29.606464 180-189
CGTGT 2209430 1.3844613 24.87606 230-239
ATCTA 1497345 1.3789711 21.668976 110-119
CACGT 2255710 1.3783633 18.02866 180-189
GAGAG 2593335 1.3719752 27.07393 220-229
TCAAG 1923980 1.3698504 25.432026 150-159
GAACT 1918920 1.3662478 15.686293 190-199
AGCAG 2480240 1.3652291 14.043404 50-59
GGCAG 3003440 1.3636864 13.504347 150-159
CTACG 2231670 1.3636736 16.463129 130-139
CCAGT 2206700 1.3484155 20.599962 190-199
TGTCC 2059280 1.3425802 57.157528 15-19
TGTGT 1720875 1.3405606 15.965795 240-247
CAATT 1444640 1.3304327 24.88579 180-189
AAGAG 2073720 1.3300074 20.667786 150-159
GATTG 1803800 1.3169857 25.542713 100-109
TTACA 1429780 1.3167475 10.121869 120-129
AACCA 1896285 1.3166065 19.222334 190-199
ACATA 1514050 1.3068602 22.554728 130-139
CCTTT 1542820 1.3010705 49.41609 45-49
AATTC 1409975 1.2985082 24.824951 180-189
CGACA 2265835 1.2976702 16.314547 70-79
ATACA 1500805 1.2954279 22.555693 35-39
AGAGC 2350920 1.2940459 26.00768 220-229
GTGGC 2669315 1.2931228 15.341029 40-44
ACAGT 1815280 1.2924573 13.106912 120-129
TGGAA 1881795 1.2877178 19.729683 120-129
ACGTC 2106330 1.287084 19.833897 180-189
TCACT 1625255 1.2845845 14.859175 190-199
GTGCG 2650100 1.2838143 17.43519 240-247
TCCGT 1963410 1.2800763 13.855078 150-159
ATCCG 2088545 1.2762164 25.55573 70-79
ACATG 1791535 1.2755511 14.637345 190-199
GGCCT 2530415 1.2754278 68.297844 3
TTATT 1038330 1.2683804 20.374037 240-247
AGACC 2212095 1.2668929 104.05724 9
TGCGA 2156590 1.2665542 14.602527 240-247
ACAGA 1887030 1.2592357 20.065905 140-149
ATATT 1094845 1.2534944 26.651302 240-247
AGTCG 2133220 1.2528292 17.028072 150-159
CCTAC 1957580 1.2445841 17.028719 190-199
CGGCT 2443915 1.2318283 22.084715 230-239
ATTAG 1390895 1.2311287 27.075727 110-119
GATCC 2004630 1.2249397 25.411428 70-79
ATTAT 1063925 1.2180939 8.795629 50-59
GTCGA 2071735 1.2167194 13.259866 150-159
ACACA 1742670 1.2099504 7.7896976 130-139
CTTCG 1848470 1.2051393 110.70045 3
AGCTC 1946760 1.1895779 13.853277 210-219
ACGCA 2070615 1.1858654 22.249956 140-149
CTTCT 1403380 1.1834798 22.737423 30-34
TGGAC 2014690 1.1832173 15.90539 80-89
TTGGG 1962165 1.1817126 16.486563 100-109
TACCA 1594790 1.1814092 10.52186 45-49
TCGGA 2008210 1.1794114 99.08405 5
TCGAG 1990225 1.1688489 12.819717 150-159
GAACC 2030820 1.1630746 11.226042 180-189
ATCAT 1253865 1.1547396 15.735332 110-119
TAGAC 1618170 1.1521175 18.85739 170-179
TTCGG 1834175 1.149321 106.48457 4
GAAGC 2085905 1.1481705 14.371511 200-209
ATTCC 1452615 1.1481317 22.771164 180-189
GAAGT 1676005 1.146895 13.71266 140-149
CGCTG 2274670 1.1465224 12.891645 190-199
TGAGT 1570000 1.1462843 20.869637 90-99
GGACT 1921090 1.1282465 13.09682 90-99
GACCC 2288445 1.1248238 44.484478 10-14
GTATA 1266415 1.1209471 10.6103945 240-247
CGCAG 2368770 1.1190324 17.517763 140-149
GGGTG 2400695 1.1177706 14.3011465 60-69
CCGAT 1803680 1.1021479 24.122503 160-169
GCACC 2234860 1.0984854 12.349512 130-139
GCCCC 2601645 1.0974884 12.90482 80-89
ATCAA 1266010 1.0927632 13.19824 110-119
GCAAG 1984990 1.0926226 16.543587 25-29
GCTTC 1666215 1.0863152 29.10964 2
GATCT 1427600 1.0844848 9.333186 220-229
GGAGA 2049170 1.0840908 88.83933 7
GCTCT 1658815 1.0814908 19.016281 210-219
TAGTT 1145115 1.0814403 15.991929 50-59
CACAT 1457405 1.0796353 14.764581 130-139
ACAAG 1614420 1.0773201 17.66207 80-89
CTATA 1167375 1.0750872 14.278901 50-59
ATGAG 1570775 1.074886 21.310467 60-69
AAGGC 1948865 1.0727378 28.891027 25-29
GAGCC 2253790 1.0647146 19.10115 220-229
GTAGC 1811720 1.0640141 12.195344 50-59
CCCCT 1939060 1.0580441 9.752136 140-149
GGGAG 2415290 1.054 12.909346 5
GTAGA 1540050 1.0538608 18.913445 170-179
CTTGA 1386365 1.0531605 21.958933 90-99
AGGCC 2224590 1.0509202 13.656616 2
ACACC 1760325 1.0489461 13.261401 40-44
TTGAG 1433085 1.0463203 20.621931 90-99
AGGTC 1777955 1.044184 18.019835 10-14
CAGCA 1809760 1.0364708 12.761366 45-49
TGGCT 1648470 1.0329556 33.511436 40-44
ATGGT 1411355 1.0304549 10.656012 35-39
ATGGG 1822160 1.0285337 19.385662 100-109
CTCCG 1959005 1.027363 10.019298 140-149
CGAGT 1746685 1.0258192 16.455225 160-169
CGTGA 1742290 1.023238 20.92907 150-159
CGAGG 2252310 1.0226421 15.445595 220-229
ACCGC 2074620 1.0197237 12.244716 220-229
AGCCG 2151095 1.0162004 20.14106 220-229
GCCAA 1771170 1.01437 15.737929 180-189
TTATA 882325 1.010179 13.40433 110-119
ACTCA 1351865 1.001452 13.68438 190-199
AACTC 1347730 0.99838877 17.444132 190-199
TTTAG 1056450 0.9977056 31.471094 45-49
TTTCA 1006740 0.98922485 8.048693 100-109
AGATC 1388255 0.9884207 12.715381 210-219
ACCAA 1420000 0.9859179 18.190765 130-139
GACAG 1782815 0.9813369 15.705054 70-79
CCGAG 2073980 0.97977036 19.097067 220-229
AAACT 1134175 0.97896916 16.982504 130-139
TGAGG 1731195 0.97718775 11.73319 220-229
TACAG 1371395 0.97641665 8.990537 120-129
CATAC 1317625 0.9760872 15.079548 130-139
TCATA 1059380 0.9756298 9.390341 110-119
ATGAC 1362160 0.9698414 11.288801 160-169
CCGTC 1845860 0.96802616 10.805929 140-149
AATCA 1120095 0.9668159 15.043199 110-119
CCAAC 1616245 0.96309143 14.851291 130-139
AAGGT 1383325 0.94661325 20.177055 15-19
TAACA 1094575 0.9447883 9.262013 120-129
AGCCC 1921055 0.9442431 12.504032 80-89
CGGAC 1996070 0.94296485 11.0984535 220-229
GGTAT 1287395 0.9399496 9.521268 110-119
AACTG 1318635 0.938852 12.965062 60-69
GGGAC 2042775 0.9275046 10.08071 90-99
TGGTT 1190475 0.92737925 10.208005 50-59
TATGG 1268900 0.926446 13.738106 35-39
CCCAG 1881290 0.9246975 15.624273 80-89
ACAGG 1677945 0.923612 15.289931 70-79
GCAGT 1565160 0.91921043 7.1811423 50-59
TACAC 1240615 0.91903883 22.926773 35-39
GTTAT 968140 0.9143061 10.700271 110-119
ATAGT 1028395 0.91026753 17.194653 120-129
CAGAA 1358840 0.90676874 18.101175 140-149
TTTAT 736935 0.900209 10.9932165 240-247
ATGAT 1016260 0.8995264 8.666085 120-129
CGGAG 1979465 0.8987592 76.47638 6
AAGTT 1014820 0.8982517 17.788788 140-149
CCCCA 1753075 0.89653796 17.60197 80-89
GATAC 1252525 0.89178264 24.253838 35-39
GGTAG 1577865 0.89063936 12.823679 120-129
GCCAT 1450350 0.88624394 7.9364014 60-69
GACTG 1500160 0.8810364 12.7371235 90-99
GAGAT 1285765 0.87985265 12.360528 210-219
TATTT 709085 0.8661885 10.88493 240-247
CGTAT 1133370 0.8609712 7.7542872 240-247
GTTTC 1050210 0.8512114 5.780682 100-109
AGAAG 1325625 0.850207 16.075737 140-149
TCCTT 1003585 0.8463299 11.312892 45-49
ACGCT 1366710 0.8351352 15.858202 190-199
TATAC 903740 0.83229405 10.287939 130-139
GTCTA 1094770 0.8316485 11.130642 30-34
TCTTC 981395 0.827617 48.30408 2
GATGA 1194265 0.817239 6.7143474 60-69
ATATA 758415 0.8138275 12.300137 130-139
GCCTA 1329915 0.8126515 16.371403 190-199
CATGG 1379710 0.8102966 8.38055 200-209
ATCCA 1090660 0.80795324 9.427232 180-189
GGTTC 1289260 0.8078693 11.831563 160-169
TTACC 1018200 0.80477464 9.059034 45-49
TGGCA 1348645 0.7920523 5.565017 100-109
AGTCT 1039730 0.78983706 12.999284 8
GATCA 1108235 0.78904986 8.821319 110-119
CATTA 855730 0.7880795 13.349591 110-119
GCTTG 1257565 0.7880087 16.863102 90-99
TTATG 829845 0.783701 12.00427 50-59
ATATG 884875 0.78323305 5.804263 50-59
CCTCC 1429535 0.780023 10.537915 140-149
GTCTG 1241825 0.7781458 14.360283 1
ACAAA 960830 0.77730435 13.047679 130-139
GGTTA 1063355 0.77637404 9.100299 50-59
GGTGC 1599710 0.77496344 11.419282 60-69
GTTCA 1016980 0.77255493 14.461618 160-169
ACATC 1037785 0.76878375 5.940754 110-119
TCATC 968970 0.7658638 9.584113 110-119
GACCG 1617350 0.7640535 9.629906 210-219
CCAGC 1551505 0.76260054 6.8620415 80-89
CTATC 960585 0.75923634 7.5002704 110-119
GCGGA 1668850 0.7577271 9.672359 220-229
CCCCC 1712345 0.75156635 14.249507 80-89
AACGC 1297380 0.7430248 15.389637 180-189
TATGA 839375 0.74295944 5.399513 50-59
TGATG 1005310 0.73399436 11.670992 120-129
ACGCC 1482545 0.7287051 13.693525 180-189
GAAAT 876910 0.7274778 8.071534 110-119
CAACG 1268885 0.7267053 15.681352 180-189
TCAAC 979020 0.72525114 7.574757 110-119
TATAG 816525 0.72273415 7.00965 120-129
GGGAT 1273300 0.718725 13.362122 100-109
CGCCG 1768790 0.71713996 11.684014 220-229
ATAAA 710925 0.71499807 7.7855177 130-139
CTGAC 1166550 0.7128265 9.371001 220-229
ATCTT 719330 0.7068151 13.494611 1
TGACT 919340 0.69838214 5.231348 2
TGCCA 1141395 0.6974554 7.637951 60-69
ATGCC 1138525 0.69570166 8.461267 50-59
GCCGC 1709695 0.69318044 12.903768 220-229
GACCA 1205940 0.69065607 6.1758757 170-179
GGGCA 1520040 0.6901612 13.51029 150-159
TATTG 727160 0.68672585 6.2652826 240-247
TCCGG 1354370 0.68265533 11.834744 70-79
CATCC 1070280 0.6804592 6.3484774 110-119
CACAA 979595 0.68014103 8.372984 130-139
AATAC 784655 0.6772791 11.5527935 130-139
CCCGT 1290300 0.6766733 10.660868 140-149
TTGGA 926700 0.67659986 5.994505 60-69
TTTAC 687675 0.6757109 12.295479 45-49
GGATA 986025 0.6747398 15.105545 35-39
TTTCT 636985 0.66780746 9.359213 4
CAAAC 950130 0.6596832 13.650426 130-139
GATTA 742860 0.65753067 5.1972466 50-59
CACGC 1335025 0.65619564 12.781758 190-199
AAATA 650140 0.6538648 12.865906 130-139
AAGCA 963885 0.64321095 6.2464814 120-129
TCAAT 696795 0.64170927 9.081234 110-119
TAGCT 842960 0.64035964 13.4549 50-59
GCGAC 1346855 0.63626873 7.0866275 70-79
TACTT 645415 0.6341861 11.707791 1
ACTTC 788590 0.6232932 31.663631 4
TTCAT 630335 0.61936843 5.1231413 100-109
GACGA 1121290 0.6172056 9.696698 220-229
CCGGC 1520270 0.6163797 9.547422 70-79
ACCCG 1246365 0.6126172 9.772487 140-149
CAGTC 999220 0.6105786 5.229589 210-219
ACCTA 812795 0.60211277 8.156531 130-139
AGGCA 1091545 0.60083264 6.073362 80-89
CCCTA 937490 0.5960344 11.890961 110-119
AGATG 868050 0.59400916 5.9570365 120-129
TTAGC 780280 0.5927444 10.334483 45-49
AATAA 579835 0.583157 7.299411 130-139
TCCAC 914155 0.5811986 9.816935 180-189
CACCG 1170830 0.57549 9.176353 45-49
CGGTT 910350 0.5704387 11.749541 160-169
CCGTA 930380 0.5685136 6.616322 230-239
ACGAC 988135 0.5659166 9.054347 220-229
CACGA 987795 0.56572175 8.464101 180-189
GGCCC 1387210 0.5624318 9.873023 70-79
AATGG 818625 0.56018746 6.474837 120-129
GTTCC 843945 0.55022335 6.7831936 140-149
CGGCA 1162410 0.5491349 10.988199 70-79
CTGCG 1089045 0.54892117 12.747691 25-29
TAATA 511490 0.54886127 7.923009 130-139
CTTTG 676605 0.54839885 6.7282457 45-49
GGGGA 1239610 0.5409491 7.591467 4
AAATC 622035 0.5369128 8.597408 110-119
ATTTC 545475 0.5359849 6.353658 240-247
CTGTT 648520 0.5256355 5.928109 240-247
ATTAA 489460 0.5252217 6.2847214 110-119
TCAGG 887190 0.52104217 5.2946205 150-159
TTCAA 565500 0.5207939 5.4413056 200-209
ACATT 564950 0.52028733 6.9672985 110-119
GGTGA 919870 0.51922846 5.8383307 120-129
TATGT 545370 0.51504445 6.959204 140-149
TGATT 544170 0.5139111 5.6079655 50-59
GGAGG 1169415 0.51031697 5.418653 6
ACCCC 995360 0.5090358 9.2898 140-149
CCACG 1033990 0.50823 7.4001703 180-189
GCTAC 829920 0.5071269 7.733808 50-59
CGCGG 1297410 0.5055693 7.0462136 220-229
GTCCT 773190 0.50409347 7.0788403 45-49
AGTAT 566090 0.5010655 5.9778194 110-119
GGGTA 877865 0.4955184 5.3184433 100-109
GTAAC 693270 0.49359983 7.3001614 120-129
AAGAT 593455 0.49232566 5.5931997 120-129
CATAA 562700 0.48569745 6.272476 110-119
TGACG 807605 0.47430232 8.587165 220-229
CAATC 639205 0.47351852 6.7993784 110-119
GAAGA 735075 0.47144997 5.24401 15-19
GGTTT 604540 0.47093627 6.0737653 100-109
AGTTT 497480 0.46981734 5.036982 140-149
GGGTT 779560 0.4694895 6.1928363 100-109
CCGGT 925370 0.4664225 9.12033 160-169
CCGCG 1145875 0.46458468 7.315917 220-229
AACAT 529785 0.45728672 6.1399856 110-119
TGGCC 901105 0.45419207 14.419642 2
CATAG 632400 0.4502611 5.239247 120-129
CCATT 569610 0.45021373 5.2602615 110-119
TAAAT 398920 0.42806652 7.7855945 130-139
GCATG 726875 0.42688996 8.759946 60-69
TGCGC 842600 0.4247032 9.528232 25-29
ATGCT 558015 0.42389944 6.5466795 50-59
TGCTA 546895 0.41545203 5.795822 50-59
GCGCT 822530 0.41458714 8.727695 25-29
CTAAC 555995 0.4118772 5.993172 110-119
GCCTT 624950 0.40744606 10.039503 1
CCTAA 546215 0.4046322 8.176965 110-119
ATTCT 406555 0.39948177 7.4026647 2
ATGGC 655035 0.38469878 7.04991 1
ACGAG 690070 0.37984377 7.3377023 180-189
CACTT 480565 0.37983352 7.122466 3
GGTAA 554485 0.37943572 7.6159945 120-129
CATGC 591795 0.36161944 6.517065 60-69
AGCTT 472900 0.35924134 7.46257 1
TACCT 454095 0.35891193 5.1247134 2
TTTGA 379315 0.358223 5.7549357 45-49
TTCTT 333990 0.35015106 19.995684 1
GAGGC 761690 0.34583884 5.330034 35-39
ATGTG 464185 0.33890957 5.2869816 140-149
TGGGC 678550 0.32871673 9.369487 1
TGCTG 511850 0.32073274 14.248813 1
CGAGC 671370 0.31716236 6.16047 180-189
CTCTT 353690 0.29826915 9.2646475 1
CGCTT 452045 0.2947179 6.1380706 1
GCGAG 638900 0.2900871 5.414907 240-247
TCACA 370960 0.27480456 5.7201834 5
GACTT 357900 0.2718809 7.25048 1
AACTT 294370 0.27109832 11.264713 3
ATTGT 282650 0.2669331 5.119949 240-247
TTCCT 314095 0.2648784 6.448154 4
CCCTT 388360 0.2634411 6.190702 3
GAGGT 465480 0.26274413 6.8505983 7
CTCGG 505495 0.2547892 5.30813 6
GTCTT 310885 0.2519771 12.413893 1
TAACT 272870 0.25129807 7.5069456 2
AATCT 259370 0.23886527 7.566099 2
TTGGC 368830 0.23111433 7.073704 1
TGTAA 251800 0.22287677 6.539374 25-29
CGGGG 586060 0.2194933 7.6664066 3
CCTCG 398865 0.20917717 5.7117977 5
TGCTT 246075 0.19944759 11.837154 1
TCGGT 292985 0.18358871 6.3370824 7
TGCCT 265945 0.17338707 5.2669816 4
TAGGC 241195 0.14165261 6.59936 1
TTGCT 143715 0.116483234 5.7258587 4
Produced by FastQC (version 0.10.1)