FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_18_SL248347.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_18_SL248347.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5363659
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 6159825 8.456161 59.73937 110-119
ATACA 5628440 7.3211765 36.30469 110-119
CACAG 8158620 7.3156867 41.307823 110-119
TCCCT 9575325 7.053895 106.669495 30-34
CAGTA 6929540 6.5577483 38.709667 110-119
AGTAA 5332365 6.209834 46.45971 110-119
GGAGA 8447680 6.071701 92.45777 35-39
CCCTG 9604750 6.0022883 70.07094 30-34
GGTGA 9443620 5.8212147 102.28458 45-49
GTAAT 5819460 5.81226 39.083366 110-119
AATAC 4090285 5.3204255 38.449837 110-119
GTAGT 7107570 5.164651 26.745361 80-89
AGACG 6383385 5.124561 99.57221 40-44
ACAGT 5370345 5.0822086 35.103203 110-119
GTGAC 7206215 4.9615235 83.694626 45-49
GAGAC 5871665 4.7137537 139.55762 40-44
ACTCC 5471610 4.6998982 121.40912 30-34
CCCCA 5762090 4.68966 100.54795 60-69
ACCAG 5121075 4.591975 92.71911 45-49
CTCAG 5957870 4.581752 34.318096 140-149
GGCCC 8340525 4.4216003 59.71754 60-69
TAGTA 4374800 4.369387 33.516914 80-89
GCACA 4854325 4.352785 33.797222 110-119
GTGTC 7323820 4.3246174 23.86077 120-129
CGTGT 7276020 4.296392 23.237055 120-129
CACTC 4993645 4.2893453 97.72819 25-29
TGACC 5529845 4.2525907 142.03412 45-49
CTCCC 5966710 4.164841 69.73656 30-34
AGTAG 4840395 4.101082 27.55301 70-79
GACCA 4459595 3.998838 104.8407 45-49
ATGCA 4199895 3.9745574 94.705826 25-29
CCCAG 5384895 3.9237907 85.963806 60-69
TGAGG 6091885 3.7551458 68.52525 35-39
CAGGG 6312075 3.6866784 36.34534 50-59
AGGAG 5091180 3.659243 92.722855 35-39
ATGGT 5022725 3.6497173 25.458555 190-199
CCACT 4245230 3.646486 26.935259 240-247
GACGG 6241005 3.6451688 106.57059 40-44
ACGGT 5282870 3.6372886 94.779625 40-44
TACAC 3423250 3.618443 26.682043 110-119
CTGAG 5233425 3.6032453 105.03371 35-39
TGTCC 5400820 3.5620704 20.457466 120-129
TGCAC 4594225 3.533075 114.80536 25-29
AGATA 3031800 3.5306988 19.194157 150-159
CCCTT 4760850 3.5071957 33.763203 20-24
AAAAA 1876860 3.5029368 22.327925 130-139
GGGTT 6616375 3.4978197 43.257706 50-59
GAGGA 4808450 3.4560335 121.70793 35-39
GCCCC 5787745 3.4271111 73.17689 60-69
TGGTG 6401275 3.3841047 18.01427 190-199
CCTGA 4379890 3.368246 83.888504 30-34
CCAGG 5106510 3.3313482 41.227516 50-59
GGATG 5356975 3.3021343 69.5375 20-24
TCTTG 4724385 3.2885165 17.901085 170-179
CTTCA 3615230 3.277338 16.596872 200-209
GCACT 4255625 3.2726827 88.7421 25-29
CCGTG 5841010 3.26803 23.422121 120-129
CTCTC 4418040 3.254656 13.5977335 130-139
CTTGG 5490330 3.2419658 19.226946 20-24
AGGCT 4706605 3.2405264 23.72826 30-34
CAGAT 3391745 3.2097673 17.870132 150-159
CCTGG 5721680 3.2012653 29.88657 50-59
TCAGG 4635810 3.1917837 20.658976 140-149
TGTCT 4485675 3.122357 19.763369 180-189
TCGCA 4054665 3.1181395 23.86098 100-109
TCTCA 3376360 3.0607936 21.459692 130-139
TTCCC 4134355 3.045673 50.351536 50-59
GGTTG 5754420 3.0421376 75.0182 15-19
CGCAC 4174075 3.0415068 24.37022 100-109
CGGAT 4390930 3.0231824 78.95235 20-24
GGTTC 5032180 2.9714348 48.7605 50-59
ACAGA 2680565 2.9578385 13.837208 140-149
TGGCC 5272580 2.9499948 44.27083 60-69
AGGGT 4760910 2.9347088 50.659023 50-59
GATGC 4251235 2.9270015 96.951805 25-29
ACCAC 2872120 2.8765616 18.990355 230-239
TCTCT 3650815 2.8384242 15.1043215 180-189
GTTGG 5362180 2.8347757 53.3437 15-19
GCCCT 4520430 2.8249485 18.429441 20-24
AGTCA 2949650 2.791392 32.02903 70-79
TCAAA 2141945 2.7861285 57.26341 70-79
CGGTG 5551510 2.7808423 67.67098 40-44
GACTC 3609705 2.7759542 18.871975 190-199
GCCGT 4942670 2.765411 22.146957 120-129
CCAGT 3571650 2.7466886 36.798065 60-69
TTCTT 3323535 2.7270916 20.475521 170-179
CGGCC 5133925 2.7216709 12.741299 120-129
GATGG 4397610 2.7107646 18.813578 15-19
GTTCC 4093530 2.699857 55.13519 50-59
AGATG 3084910 2.6137264 16.872477 180-189
TCACA 2439710 2.5788217 11.282464 140-149
GCGGA 4306760 2.5154388 86.99603 20-24
GGCTG 5012170 2.5106783 12.393314 30-34
TGTTC 3588270 2.4976978 13.016675 140-149
TCCTC 3367795 2.4809678 16.985933 130-139
TCCAT 2736245 2.4805057 16.36576 70-79
AGGGA 3424740 2.4615033 8.7391615 150-159
TGGAG 3992390 2.46098 15.996941 25-29
GGCGG 5767100 2.4506319 42.697445 20-24
ACAGC 2688605 2.410823 10.975157 120-129
GAGAT 2823955 2.392629 16.44499 180-189
TTGGG 4524015 2.3916705 79.993614 15-19
CTGGC 4252640 2.379341 35.88967 60-69
ACTCT 2609370 2.3654892 13.118631 200-209
GTTCT 3389595 2.3594053 17.483992 160-169
AATAA 1463000 2.3417845 9.678532 110-119
TACCA 2205845 2.331622 15.85928 230-239
CACGG 3544155 2.3121102 9.45238 120-129
CTGGA 3343295 2.301879 14.333075 180-189
CAGCT 2985625 2.2960207 11.966479 150-159
CATTT 2357335 2.2553742 17.645082 150-159
TCATT 2354900 2.2530444 16.601807 150-159
TGTAG 3068615 2.2297814 7.7249885 210-219
TTCAC 2449520 2.2205794 18.334875 200-209
TTCAT 2314445 2.2143393 16.036222 140-149
TCAGC 2862945 2.201677 14.270545 140-149
ACTAC 2064715 2.1824446 12.394431 230-239
GTGAA 2574365 2.1811612 17.709692 190-199
CTACT 2387595 2.164442 7.398819 230-239
CAGAG 2695505 2.1639428 9.105737 140-149
TTGTC 3108275 2.1635864 13.256095 170-179
TCTGC 3265180 2.1535249 11.918125 210-219
GATAC 2267825 2.1461492 14.421038 150-159
CACTA 2017355 2.132384 15.039048 240-247
TGGGG 4730540 2.121501 52.871517 15-19
ATTTG 2476110 2.1209695 15.449679 150-159
TAGTC 2565830 2.082478 26.58304 70-79
CTCGC 3322570 2.0763707 16.314135 100-109
GCTGT 3511635 2.0735734 10.1122875 140-149
TCTCG 3128710 2.0635173 16.819569 100-109
TTGGA 2836610 2.0611968 15.594538 170-179
CTCTG 3119240 2.0572712 14.481503 180-189
ATCTC 2263130 2.05161 9.031955 100-109
ACACA 1662925 2.0495265 13.313458 110-119
GTCTC 3097255 2.0427713 11.8589945 180-189
GTCCT 3089985 2.0379763 14.489454 130-139
ATATA 1482855 2.0356522 10.605124 110-119
GAATC 2149070 2.0337656 14.075218 190-199
TACAG 2141440 2.026545 13.579703 160-169
GTTCA 2491825 2.0224142 15.06697 140-149
GAACT 2130940 2.0166085 10.3695965 160-169
GTCAA 2120450 2.0066812 40.59913 70-79
CAGGC 3069575 2.0025072 15.407801 140-149
AGCTC 2584945 1.987888 12.140134 140-149
AACCA 1610765 1.9852402 15.235331 70-79
ATGTA 1986275 1.9838173 7.1626387 70-79
TTGCA 2438700 1.9792969 14.219612 150-159
TGAAT 1981070 1.978619 13.4908085 190-199
GATAT 1980745 1.9782944 7.904193 180-189
AATAT 1433790 1.9682958 13.273229 110-119
AATCG 2072695 1.9614882 13.001785 190-199
CCTTC 2653385 1.9546804 13.87628 200-209
ACACG 2176075 1.951247 13.100521 120-129
TTTGC 2790090 1.9421061 12.242076 150-159
ACGGC 2962840 1.9328761 14.291673 120-129
GAGTC 2789050 1.9202778 12.100242 210-219
TGGAA 2248990 1.9054834 9.086787 170-179
TCGGC 3393545 1.8986794 7.8983765 130-139
GGGGC 4454960 1.89306 48.218376 15-19
TACTA 1693420 1.8891209 9.82189 230-239
ATATG 1885360 1.8830272 8.124336 180-189
CAGCC 2558520 1.8643067 10.003909 120-129
CATAG 1957420 1.8523983 8.203725 210-219
GGGCG 4336690 1.8428031 60.300358 20-24
CTGTT 2595500 1.8066571 12.758551 140-149
AGTAT 1795735 1.7935133 11.651256 220-229
GTTGT 2844690 1.7727888 6.780892 210-219
CGTAG 2550905 1.7563138 9.527587 210-219
GACGT 2536485 1.7463856 9.515309 170-179
GCAGA 2159735 1.7338282 14.37197 150-159
CTCCA 2018360 1.7336919 7.838657 150-159
CCAGA 1931975 1.7323669 25.382797 60-69
GTCAC 2252305 1.7320794 8.372222 130-139
ATTGT 2018920 1.729353 7.5635185 170-179
TGCAG 2510485 1.7284844 13.53955 150-159
CACCA 1717275 1.7199308 13.138598 230-239
AGAAC 1547105 1.7071353 10.985981 160-169
AGTCT 2102645 1.7065481 12.956739 210-219
CAAAA 1104425 1.6750467 20.674866 70-79
GTCTG 2821880 1.6662823 9.004529 210-219
ATAGT 1659715 1.6576616 7.275622 220-229
GGTCA 2400175 1.6525352 7.318955 130-139
TGGTT 2649785 1.6513255 18.259403 10-14
CCTTG 2502100 1.6502413 21.863539 20-24
TTCAG 2029065 1.646829 9.218476 150-159
TCAGA 1724635 1.632103 9.993477 130-139
TGACT 2001335 1.6243229 11.312735 190-199
TCTGG 2735385 1.615208 11.93059 180-189
TCCCA 1878535 1.6135877 10.456178 240-247
AACTG 1683475 1.5931513 10.23497 160-169
TGTGC 2697300 1.5927194 8.078772 220-229
CTGTG 2690925 1.5889549 5.2703476 210-219
GAACC 1767515 1.5848986 12.879841 70-79
GCTCC 2534075 1.5836173 6.4010625 150-159
TGCTC 2393035 1.5783083 5.280647 150-159
CAAAG 1429145 1.5769736 29.061161 70-79
GAGAA 1594985 1.5756943 10.055186 160-169
GTGCT 2651755 1.5658257 7.920692 220-229
AATAG 1343005 1.5640035 9.322736 240-247
CTGGT 2647355 1.5632274 6.8746614 160-169
GTCCC 2495840 1.5597231 19.502851 50-59
TTGTA 1817535 1.5568521 8.612146 210-219
TTGGT 2494580 1.554603 23.531921 25-29
TAGTT 1813440 1.5533442 9.454031 220-229
ACTGA 1638415 1.5505089 8.398406 180-189
GGCCG 3266555 1.5503985 13.203363 120-129
ATAGA 1313790 1.5299811 9.672585 240-247
GGACG 2600880 1.5190898 7.098546 170-179
ACCAT 1429425 1.5109305 11.271456 45-49
GCTCA 1956415 1.5045323 10.881677 140-149
CCACC 1847310 1.5034919 9.588163 230-239
CCCAC 1831885 1.4909378 10.188367 240-247
AAATA 928045 1.4854968 5.1999454 9
GCTCT 2248145 1.4827471 9.982614 130-139
CCTCG 2353290 1.4706395 9.698406 130-139
CGACT 1837880 1.413376 8.396037 190-199
GCTGA 2048490 1.410398 20.19193 30-34
CAGAC 1569435 1.4072839 14.609117 60-69
CACTG 1826210 1.4044015 7.045076 230-239
AATTG 1403830 1.4020929 9.18091 170-179
TATAC 1253725 1.3986124 6.7316723 110-119
CTGCT 2113100 1.393679 5.782144 90-99
CATGT 1715750 1.3925365 7.1947722 70-79
CTCTT 1784145 1.3871313 7.9306636 200-209
TCGAC 1792935 1.3788121 9.014129 190-199
TGATA 1379485 1.3777782 7.380042 180-189
TGGAC 1998265 1.3758178 10.150167 170-179
GCGTA 1997985 1.3756248 10.090747 210-219
GAGGG 2598300 1.3586876 7.953562 200-209
CTTGA 1669145 1.3547108 8.137727 200-209
CCTCA 1572005 1.3502907 7.289994 130-139
GGGAG 2581400 1.3498504 6.345313 150-159
AGATC 1413275 1.3374484 6.9330425 130-139
ACGGA 1659410 1.3321689 9.253511 200-209
GTAGG 2138215 1.3180336 5.083934 160-169
CCATC 1529680 1.3139352 10.539982 240-247
ACTTC 1445280 1.3101991 6.508525 220-229
CTGAT 1602645 1.3007382 7.1307034 180-189
GAATT 1301890 1.3002793 10.006021 170-179
ATCGG 1880080 1.2944467 7.7242203 190-199
CTCGA 1680505 1.2923506 8.09568 190-199
GTGTT 2064485 1.286571 7.6412954 160-169
GGAAT 1517435 1.2856647 8.45066 170-179
TATGG 1768195 1.2848427 5.9158125 180-189
GTGGA 2080470 1.2824385 20.404245 25-29
TCACG 1650100 1.2689686 9.594682 200-209
GGAGT 2057950 1.268557 6.9977174 210-219
CCATG 1645425 1.2653733 7.3136425 70-79
TCCAC 1470505 1.263106 9.16941 240-247
AGCCG 1932190 1.2605082 8.903627 120-129
ATAAA 779930 1.2484131 6.13251 7
GGCTC 2229460 1.247377 8.268075 130-139
ACGTG 1810385 1.2464613 9.107034 170-179
AAGTA 1062650 1.2375147 15.515147 70-79
ATAAC 950230 1.2360089 7.666116 80-89
AAAGT 1058915 1.233165 21.262505 70-79
ACCGT 1601465 1.2315669 17.480947 45-49
ACTAT 1102655 1.230084 6.0254407 230-239
GGAGG 2344355 1.2258964 15.71599 30-34
GATCT 1486355 1.206355 5.45302 130-139
ACTCG 1563170 1.2021172 8.273825 190-199
GTGGT 2271870 1.2010491 10.109085 50-59
GTCCA 1552880 1.1942037 15.466268 70-79
TTGGC 2008320 1.185886 7.9668756 60-69
TTGAG 1627265 1.1824374 7.7792764 200-209
GCATA 1247615 1.1806766 7.957156 210-219
TGGTC 1984745 1.1719652 11.473565 50-59
TTCCA 1291140 1.1704656 7.3436284 230-239
ACTGG 1699595 1.1701816 6.933419 160-169
AAAAG 852530 1.1576241 13.74621 70-79
AGAGC 1437220 1.1537956 6.71344 140-149
TATCA 1031385 1.1505775 6.8448176 70-79
TGCCC 1835500 1.1470574 6.437748 60-69
AGCTT 1406625 1.1416446 5.993203 150-159
ACTGT 1399835 1.1361337 5.5174294 230-239
ATACC 1065450 1.1262017 6.880822 70-79
ATATC 1008415 1.124953 7.3025713 70-79
TCTGT 1609970 1.1206564 6.4903784 210-219
GTCCG 1985125 1.1106722 5.056105 130-139
CAATA 853150 1.1097324 6.392148 100-109
GAAGA 1122835 1.1092548 18.152906 35-39
AGTTC 1364920 1.107796 5.230777 160-169
AGGTT 1510405 1.0975219 22.792166 10-14
CGGCT 1949940 1.0909863 7.790942 130-139
CCATA 1030605 1.0893699 9.5515995 70-79
TCTAC 1201455 1.0891628 7.0069637 180-189
CTCGG 1936115 1.0832512 8.43534 130-139
GTCTA 1332650 1.081605 7.099435 180-189
CTGAA 1142915 1.081594 14.347852 35-39
TATGT 1246435 1.067663 7.433886 220-229
GCTTC 1609585 1.0615897 5.384168 150-159
AAAAT 663060 1.0613422 5.0907264 8
CTGCG 1884270 1.054244 6.754741 210-219
TACTG 1298320 1.053742 6.2121806 180-189
CTTCT 1350780 1.0502002 5.36916 210-219
AGGGG 1999850 1.0457497 5.102887 60-69
CTGCC 1667410 1.042013 5.8445334 200-209
AGAGA 1050970 1.038259 12.453383 35-39
GAGCT 1507910 1.0382053 6.0482774 140-149
GGGGT 2313480 1.0375243 7.8086295 10-14
ATCCA 981075 1.0370157 14.448184 240-247
CTACC 1202020 1.032488 8.440398 230-239
GCGGT 2032275 1.0179999 5.726097 130-139
ATGGG 1641785 1.0120254 29.37067 15-19
CATGG 1467475 1.0103656 5.270544 190-199
ATACG 1062160 1.0051718 5.160199 120-129
AACTT 900190 1.004221 5.8423705 200-209
TAGAT 1000900 0.9996617 7.495946 240-247
TGCGT 1690970 0.9984951 8.100969 210-219
ACGTC 1284980 0.98818207 11.077438 70-79
CAGCG 1512460 0.9866877 6.3560624 160-169
CGAAC 1096690 0.98338205 13.439333 70-79
TATAA 712715 0.9784097 6.1956077 240-247
AAGAG 989690 0.9777202 13.426693 35-39
TCCCC 1384760 0.96658045 5.0521913 90-99
CGGAG 1640660 0.9582563 6.6248446 210-219
AGCTG 1375110 0.9467717 5.0042953 90-99
ACAAT 727430 0.9462024 7.3793116 100-109
TGTAA 939800 0.93863726 6.556652 230-239
TTCTG 1342540 0.93450576 5.417259 210-219
TAGGC 1352995 0.9315454 5.772096 160-169
ACCCA 926585 0.928018 12.298258 240-247
ACTAA 711755 0.9258132 12.158983 240-247
CCATT 1016525 0.921517 5.7725706 50-59
GAGTT 1261525 0.9166755 5.032515 160-169
TGAAC 968630 0.91665995 6.5289383 190-199
CGTGG 1819745 0.9115402 7.0201387 50-59
TCGAA 962720 0.911067 16.942244 70-79
TGCAT 1116695 0.90633166 6.1104593 210-219
CCCAA 896910 0.8982971 9.273401 240-247
CATCA 837450 0.8852012 5.6345563 230-239
GTATG 1206375 0.8766014 5.21516 220-229
AGCGT 1257120 0.86553484 6.4733624 160-169
TGAAG 1020000 0.864207 9.472081 35-39
GGTGG 1910720 0.8568989 20.584377 25-29
CCAGC 1167455 0.8506848 5.6429615 80-89
CGTCC 1353830 0.8460478 9.291879 70-79
CCAAT 800240 0.8458695 10.155975 240-247
ATCAA 647685 0.84247434 12.485308 70-79
GAGGC 1435340 0.8383355 13.271708 30-34
AAAGA 615620 0.8359314 5.6528487 8
CGGTC 1485645 0.8312144 5.393538 130-139
CTTCC 1116055 0.822169 5.227919 230-239
TAACC 757380 0.80056566 6.126305 80-89
GGGCC 1673130 0.7941143 15.6553755 15-19
AACCG 884510 0.7931241 5.7232523 190-199
TGAGA 917880 0.7776847 5.3815904 240-247
GACCG 1188055 0.77505475 16.295404 45-49
AAAGG 764045 0.75480425 8.611195 10-14
TGGGC 1479795 0.7412537 16.976135 15-19
GTCGA 1072475 0.73840564 12.260969 70-79
GGTCC 1314145 0.7352607 11.77817 50-59
CTAAT 657640 0.7336405 9.412066 240-247
AGCCA 815435 0.7311858 5.8479557 80-89
ATGAT 730025 0.7291218 6.703596 240-247
AATCC 662970 0.70077235 8.859533 240-247
GTAGC 1017750 0.70072716 5.310699 80-89
GGAAG 964365 0.69312936 6.0985627 30-34
CATAC 654130 0.69142836 8.195588 70-79
AAAGC 617285 0.6811361 9.434618 70-79
AAGGT 799545 0.6774239 9.594659 10-14
GAAAA 496845 0.6746505 5.8660054 8
CATTG 820080 0.665593 5.2440476 50-59
TAGGT 896020 0.6510848 5.3851643 10-14
AGGAT 766525 0.64944726 5.7087398 10-14
TATGA 639950 0.6391582 5.40429 240-247
GAGGT 1025550 0.6321671 6.6352973 10-14
AGAAG 630560 0.6229337 5.0092063 9
CAATC 587705 0.6212158 8.625212 240-247
ATAGG 727875 0.6167007 5.2309012 10-14
TAAAA 383950 0.6145785 6.317408 8
GGGTC 1211015 0.60661733 6.0116463 70-79
CGATG 860710 0.59260416 13.085051 15-19
AAGAA 435105 0.59081566 5.2330265 9
AATGG 685870 0.58111143 6.3096485 10-14
AAATG 490130 0.5707835 5.1396494 9
CATCC 648860 0.5573453 6.9836035 240-247
AAGCA 483565 0.5335843 6.3125906 70-79
GAAGG 736545 0.5293856 5.1625643 10-14
AAGGG 726190 0.5219431 6.2838187 10-14
GGTCG 1015830 0.5088459 6.1077685 70-79
TGATC 618660 0.5021166 5.3872437 240-247
GATCC 618830 0.47589588 5.439658 240-247
ATAAG 403740 0.47017756 5.2748656 9
ACGAT 476930 0.4513412 5.5903926 10-14
CGGTT 760025 0.44878453 7.9578276 10-14
TAAGG 518700 0.4394747 5.68488 10-14
AAGCT 416835 0.39447048 7.600379 30-34
TGGGT 746085 0.39442605 5.100489 10-14
Produced by FastQC (version 0.10.1)