FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_4_SL248350.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_4_SL248350.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12270222
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 14176355 8.736516 62.787876 110-119
ATACA 12891530 7.5223565 39.347755 110-119
CACAG 18874335 7.4813333 42.733173 110-119
TCCCT 22408325 7.1563783 109.35689 30-34
CAGTA 16219005 6.78978 41.225723 60-69
AGTAA 12213425 6.365496 48.585308 110-119
GGAGA 19595575 6.196637 94.56856 35-39
CCCTG 22697915 6.130431 71.78216 30-34
GTAAT 13440290 5.942434 40.413315 110-119
GGTGA 21728865 5.8290253 101.47222 45-49
AATAC 9433365 5.5044775 41.457317 110-119
GTAGT 16355040 5.187874 24.835356 70-79
ACAGT 12327210 5.1605535 35.99474 110-119
AGACG 14464665 5.1210737 101.49822 40-44
GTGAC 16731225 5.0250616 83.86955 45-49
ACTCC 12858710 4.8408356 125.11145 30-34
GAGAC 13422550 4.7521234 141.33243 40-44
CCCCA 13293130 4.7383385 101.26956 60-69
ACCAG 11869300 4.704705 97.88957 45-49
CTCAG 13850710 4.6573653 35.216557 140-149
CACTC 11949690 4.498623 102.643234 25-29
GCACA 11092555 4.3968225 34.254875 110-119
TGACC 12900240 4.337765 143.3737 45-49
GGCCC 18949635 4.3283963 59.683464 60-69
GTGTC 16964265 4.322247 24.26003 120-129
CGTGT 16934665 4.3147054 23.78614 120-129
TAGTA 9673380 4.276948 30.985575 80-89
CTCCC 14031335 4.2428603 70.9029 30-34
GACCA 10520010 4.1698794 109.58916 45-49
ATGCA 9927105 4.155795 99.13039 25-29
AGTAG 11041230 4.128519 28.929153 70-79
CCCAG 12568700 4.001607 86.764465 60-69
CCACT 10297120 3.8764904 28.162619 240-247
TGAGG 14278880 3.830479 69.23138 35-39
CAGGG 14984680 3.8061175 37.193382 50-59
TACAC 7980650 3.740455 28.996277 110-119
AGGAG 11825010 3.739379 95.07052 35-39
ATGGT 11592430 3.6771579 25.459759 190-199
TGCAC 10884600 3.659997 119.91013 25-29
ACGGT 12167200 3.6543007 96.2433 40-44
GACGG 14371790 3.650443 107.717636 40-44
CTGAG 12139465 3.64597 105.79134 35-39
GGGTT 15797885 3.595165 44.30438 50-59
AGATA 6840505 3.5651922 19.378736 150-159
GAGGA 11109785 3.5132067 124.603645 35-39
TGTCC 12273060 3.5009193 20.992952 120-129
CCTGA 10333200 3.4745862 87.041405 30-34
CCAGG 12016890 3.417287 42.26296 50-59
GCCCC 13321920 3.4068081 72.688385 60-69
GCACT 10083340 3.39057 92.94207 25-29
GGATG 12563785 3.3703845 72.59658 20-24
CCCTT 10546040 3.3680096 32.109497 20-24
TGGTG 14792420 3.3663487 17.774088 190-199
TCTTG 11056485 3.330968 18.092426 170-179
CTTCA 8199820 3.2602541 16.691713 200-209
CCTGG 13484185 3.2529323 30.765076 50-59
CTCTC 10072880 3.2169 13.823685 100-109
TCAGG 10671230 3.2050002 20.945728 140-149
CCGTG 13269030 3.201028 23.90859 120-129
CAGAT 7586040 3.1757524 17.646732 150-159
TTCCC 9929160 3.1710012 52.29972 50-59
CTTGG 12421855 3.1649072 18.111275 20-24
AGGCT 10521180 3.1599345 21.607634 30-34
TCGCA 9340185 3.1406803 24.871288 100-109
GGTTG 13767690 3.1331482 76.0195 15-19
TGTCT 10379760 3.1270924 19.485807 180-189
CGGAT 10385380 3.1191485 82.5198 20-24
CGCAC 9623015 3.0637636 25.593315 100-109
TCTCA 7686125 3.0560088 21.737293 130-139
GGTTC 11919200 3.0368383 50.032265 50-59
ACAGA 6110245 3.0152907 14.982134 140-149
GATGC 10038810 3.0150595 99.314804 25-29
AGGGT 11194875 3.003158 52.343845 50-59
ACCAC 6690430 2.9690397 19.909002 230-239
TGGCC 12188430 2.9403436 44.1085 60-69
GTTGG 12789960 2.9106438 54.44224 15-19
GACTC 8544810 2.873232 20.64302 190-199
CCAGT 8494435 2.856293 38.03485 60-69
TCAAA 4893820 2.8556004 59.330673 70-79
AGTCA 6780645 2.838589 33.426197 70-79
TCTCT 8203080 2.7668464 14.51522 180-189
GTTCC 9650810 2.7529163 56.74248 50-59
TTCTT 7715770 2.7486002 20.302914 160-169
GCCCT 10151565 2.7418144 17.487608 20-24
CGGTG 12694590 2.7353592 67.849815 40-44
GCCGT 11219975 2.7067132 22.443724 120-129
CGGCC 11767590 2.6879036 12.944379 120-129
TCACA 5667195 2.6561608 12.02125 140-149
GATGG 9871205 2.648068 18.093254 15-19
AGGGA 8252835 2.6097639 9.754305 150-159
GCGGA 10268475 2.6081986 90.35874 20-24
AGATG 6938680 2.5945 17.055542 180-189
GGCGG 13663700 2.4899185 43.370506 20-24
TCCAT 6211460 2.4696808 15.090267 70-79
TACCA 5204990 2.4395297 16.583134 230-239
TGTTC 8075500 2.432892 12.898749 140-149
TTGGG 10690390 2.4328394 82.23599 15-19
CTGGC 10073750 2.430197 36.372322 60-69
ACTCT 6107035 2.4281614 13.902946 190-199
TCCTC 7577480 2.419963 16.372265 130-139
ACAGC 6081865 2.410705 11.055334 120-129
GGCTG 11174665 2.4078543 11.713456 140-149
CAGCT 7135050 2.3991938 12.535367 150-159
TGGAG 8868005 2.3789475 14.730085 25-29
GTTCT 7888510 2.3765578 18.546549 160-169
GAGAT 6243645 2.334614 16.547285 180-189
CACGG 8186285 2.3279638 9.889656 120-129
TGTAG 7231455 2.2938418 8.700932 210-219
CTGGA 7577115 2.2757127 14.3329115 180-189
AATAA 3131030 2.2745733 9.041885 110-119
CTACT 5720425 2.274445 7.899121 180-189
CAGAG 6365380 2.2536008 10.041787 140-149
TCAGC 6675505 2.2446694 15.395471 140-149
ACTAC 4772610 2.236877 12.470167 230-239
CACTA 4756975 2.2295492 15.219931 240-247
CATTT 5282260 2.2181509 17.227524 150-159
TCATT 5264640 2.210752 15.930991 150-159
TTCAT 5206505 2.1863396 16.350052 140-149
TGGGG 11258930 2.166896 54.258858 15-19
GTGAA 5792990 2.1661055 17.592556 190-199
TAGTC 6085220 2.1610696 27.905964 70-79
TTCAC 5410640 2.1512744 18.26951 200-209
GATAC 5097370 2.1339178 14.719614 150-159
TTGGA 6620170 2.0999403 16.15377 170-179
TTGTC 6939905 2.0907733 13.275815 170-179
ATTTG 5560700 2.0856724 15.158683 150-159
GTCAA 4977195 2.0836089 42.527905 70-79
CTCGC 7708380 2.0819397 16.864754 100-109
AGCTC 6187255 2.080493 13.317706 140-149
GAACT 4963865 2.0780284 11.4161215 160-169
TCTGC 7281620 2.0770988 12.062254 210-219
ATATA 3353160 2.0664647 10.613307 110-119
TCTCG 7111095 2.0284567 16.317213 100-109
GATAT 4574070 2.0223603 9.05204 180-189
ACACA 3659170 2.0216587 14.27099 110-119
GAATC 4827935 2.021124 14.077557 190-199
GCTGT 7909945 2.0153384 10.021371 140-149
ATGTA 4553305 2.0131793 7.304345 160-169
CTCTG 7026505 2.004327 14.260975 180-189
TACAG 4780450 2.001245 13.513859 160-169
GTCTC 7012615 2.000365 11.53608 180-189
ATCTC 5028175 1.999206 7.783973 100-109
AACCA 3610790 1.9949292 15.842863 70-79
ACACG 5020555 1.9900274 13.767967 120-129
AAAAA 2323230 1.9895002 7.8186283 8
TGAAT 4485765 1.9833175 13.596816 190-199
GTCCT 6942240 1.9802903 13.790515 130-139
GTTCA 5551865 1.9716572 15.161545 140-149
CAGGC 6893015 1.9601921 14.984729 140-149
ACGGC 6883075 1.9573654 14.532587 120-129
AATCG 4669460 1.9547814 13.190116 190-199
ATATG 4397355 1.9442283 9.307677 180-189
AATAT 3148590 1.9403934 13.180062 110-119
TACTA 3914800 1.9378492 9.764103 230-239
GGGGC 10585835 1.929043 49.553276 15-19
TTGCA 5420635 1.9250529 14.090421 150-159
TGGAA 5147750 1.9248384 9.368119 170-179
CCTTC 6023045 1.9235346 13.767104 200-209
GAGTC 6320205 1.8982124 11.849512 210-219
CATAG 4527225 1.8952372 8.471998 210-219
CAGCC 5947345 1.8935083 10.194787 120-129
GGGCG 10288450 1.874851 60.891552 20-24
TTTGC 6183090 1.8627689 11.903066 150-159
TCGGC 7624935 1.8394436 7.7618513 130-139
AGTAT 4102030 1.8136545 11.689774 220-229
AGAAC 3656535 1.8044312 11.819397 160-169
GTTGT 6656935 1.7913195 7.4922843 210-219
CTCCA 4729150 1.7803528 8.226699 150-159
CTGTT 5856220 1.7642933 12.603598 140-149
GACGT 5840200 1.7540475 10.337454 170-179
GTCAC 5171485 1.7389357 9.39957 130-139
ACTGA 4151930 1.7381271 9.495272 180-189
CACCA 3878250 1.7210671 13.463054 230-239
CGTAG 5691330 1.7093356 9.71138 210-219
TCCCA 4538270 1.7084935 10.673159 240-247
AGTCT 4810690 1.7084404 12.8230505 210-219
GGTCA 5688030 1.7083445 8.160591 130-139
AACTG 4060810 1.6999813 11.178046 160-169
GCAGA 4794310 1.6973788 14.3175745 150-159
TGGTT 6303765 1.6962847 18.731012 10-14
ATAGT 3830815 1.6937406 7.4324045 220-229
ATTGT 4515280 1.6935629 7.634163 170-179
TGACT 4738655 1.6828583 12.0334835 190-199
GAGAA 3803690 1.6765676 10.772668 160-169
CCAGA 4228285 1.6759906 24.119347 60-69
TGCAG 5565050 1.6714087 13.381751 150-159
TAGTT 4388830 1.6461349 10.324853 220-229
TTCAG 4630605 1.6444862 9.795468 150-159
CAAAG 3329345 1.642969 30.620405 70-79
TTGTA 4374620 1.640805 9.719952 210-219
TCAGA 3906620 1.6354327 10.341036 130-139
AATAG 3136550 1.6347337 9.851061 240-247
TGTGC 6339530 1.6152197 8.166507 220-229
ATAGA 3092760 1.6119108 10.538453 240-247
CTGGT 6322105 1.61078 7.4470596 160-169
GCTCC 5958750 1.6093858 6.392098 150-159
GTGCT 6310995 1.6079493 8.587743 220-229
GTCTG 6310565 1.6078398 8.915263 210-219
TGCTC 5626760 1.6050465 5.37486 150-159
GCTCA 4725735 1.5890502 11.894811 140-149
CTGTG 6225965 1.586285 5.3977375 210-219
CAAAA 2305360 1.5857228 20.592463 70-79
GAACC 3975780 1.5759037 12.940916 70-79
GGACG 6180740 1.5699116 7.6503024 170-179
TCTGG 6141285 1.5647097 11.701477 180-189
CCCAC 4385405 1.5631782 10.343182 240-247
CCTTG 5467755 1.55969 20.691118 20-24
GGCCG 7613765 1.5533558 13.333436 120-129
TTGGT 5771675 1.5531042 22.147478 25-29
CACTG 4599195 1.5465006 7.755623 230-239
CCACC 4326130 1.5420494 9.839969 230-239
GTCCC 5531940 1.4941096 17.723282 50-59
TGATA 3332300 1.4733293 8.547425 180-189
ACCAT 3141910 1.4725835 10.791212 45-49
GCTCT 5090840 1.4521741 10.02611 130-139
CGACT 4318510 1.4521191 9.365446 190-199
TATAC 2933215 1.4519588 6.9080896 110-119
TGGAC 4790610 1.4388133 10.779603 170-179
TCGAC 4202900 1.4132445 10.035073 190-199
CTCTT 4183270 1.4109902 8.403878 200-209
GAGGG 6218395 1.4107771 8.863821 200-209
GGGAG 6208960 1.4086363 7.0347176 150-159
CCTCG 5209590 1.4070469 9.170771 130-139
AATTG 3177655 1.4049551 9.43932 170-179
CATGT 3909975 1.3885658 6.8220396 150-159
AAATA 1908660 1.3865683 5.3042192 9
CTGCT 4812250 1.3727057 6.026762 90-99
CTGAT 3845570 1.3656933 7.9746637 180-189
GCTGA 4531925 1.3611195 18.735426 30-34
CCATC 3603920 1.3567445 10.837889 240-247
ACTTC 3403840 1.3533692 6.8269353 220-229
TCCAC 3576535 1.3464351 10.293071 240-247
CTTGA 3790860 1.3462639 8.80089 200-209
CCTCA 3560390 1.3403572 7.3388495 130-139
GCGTA 4458520 1.3390733 10.103343 210-219
CTCGA 3966430 1.3337303 8.984456 190-199
AGATC 3169595 1.3268912 7.526897 130-139
TATGG 4144545 1.3146636 6.5909977 180-189
ACGGA 3707240 1.3125123 9.435107 200-209
ACGTG 4361205 1.3098454 9.97616 170-179
CAGAC 3298325 1.3073769 13.66449 60-69
GAATT 2949270 1.303978 10.411868 170-179
ACTAT 2623840 1.2988162 6.3503623 230-239
AAAGT 2479115 1.292086 22.525713 70-79
GTAGG 4810540 1.2904844 5.127986 160-169
ACTCG 3754205 1.2623688 9.213465 190-199
ATCGG 4182295 1.2561116 7.8145924 190-199
CCATG 3724535 1.252392 6.6327343 70-79
GTGTT 4652850 1.2520388 7.513864 160-169
AAGTA 2400210 1.2509618 16.350357 70-79
GGAAT 3343570 1.2502224 8.762979 170-179
ACTGG 4149280 1.2461959 7.5483503 160-169
GTGGA 4638615 1.2443634 19.30817 25-29
AGCCG 4372540 1.2434354 9.008389 120-129
ATAAC 2129115 1.2423632 7.7585297 80-89
GGAGT 4609185 1.2364686 6.9087796 210-219
TTGAG 3865800 1.2262448 8.537552 200-209
AGCTT 3449110 1.2248968 6.5048594 150-159
GATCT 3437895 1.220914 5.600506 130-139
GGCTC 5043260 1.2166389 8.240543 130-139
TTCCA 3056765 1.215372 7.763023 230-239
ATCCA 2576640 1.2076468 16.3432 240-247
AGAGC 3396295 1.2024251 7.8212004 140-149
GGAGG 5292395 1.200694 14.384603 30-34
GCATA 2864005 1.1989617 8.1464615 210-219
ACTGT 3375640 1.1988051 5.933726 230-239
ATACC 2466095 1.1558354 6.572595 240-247
ACCGT 3428100 1.1527145 15.9729595 45-49
GTGGT 5060920 1.1517265 9.131341 50-59
ATAAA 1574180 1.1435814 5.206449 7
TCTAC 2867100 1.139961 7.543487 180-189
GTCCA 3382020 1.1372198 14.213316 70-79
TTGGC 4448185 1.1333326 7.3213644 60-69
TATCA 2286155 1.1316603 6.4910383 70-79
GTCTA 3182225 1.1301169 7.610571 180-189
TGGTC 4434575 1.129865 10.408263 50-59
TCACG 3353360 1.1275828 9.435418 200-209
GCTTC 3952640 1.1274998 5.8858724 150-159
TGCCC 4173510 1.1272142 5.890823 60-69
TACTG 3158650 1.1217445 6.919398 180-189
AGGTT 3513065 1.114356 23.883638 10-14
TCTGT 3697545 1.1139529 6.575883 210-219
AGTTC 3124980 1.1097872 5.456433 160-169
CAATA 1894705 1.1055822 6.7580557 100-109
GAGCT 3657745 1.0985683 6.9463153 140-149
AAAAG 1770020 1.0874567 13.52912 70-79
AGGGG 4763520 1.0807073 5.1958265 60-69
CTACC 2847690 1.0720515 8.598465 230-239
TATGT 2849030 1.0685962 7.5190773 220-229
TAGAT 2414860 1.0676962 8.094051 240-247
CCATA 2272680 1.0651836 9.093143 70-79
GGGGT 5504130 1.059326 8.28891 10-14
CGGCT 4377695 1.0560776 7.7038174 130-139
ATATC 2131625 1.055167 7.051657 70-79
AACTT 2114700 1.0467889 5.9651146 200-209
ACCCA 2356995 1.0459734 13.527188 240-247
CTGCC 3858720 1.0421933 5.7635484 200-209
CTGCG 4316290 1.0412642 6.7632074 210-219
GAAGA 2360700 1.040535 16.124289 35-39
GTCCG 4298300 1.0369242 5.2438264 120-129
CTGAA 2474760 1.0360116 13.384097 35-39
CTCGG 4292230 1.03546 8.133532 130-139
CATGG 3444475 1.0345145 5.2856755 190-199
GCGGT 4766560 1.0270716 6.0582247 130-139
CCAAT 2171450 1.0177381 11.493792 240-247
CTTCT 3005110 1.0136044 5.816234 210-219
AGAGA 2275595 1.0030229 12.008602 35-39
ATACG 2394125 1.0022553 5.047916 120-129
TATAA 1605870 0.98965555 6.45044 240-247
TGCGT 3867520 0.98538756 8.084083 210-219
AAAAT 1348915 0.979935 5.788374 8
TGTAA 2210900 0.9775182 7.2048 230-239
CGAAC 2462805 0.9761968 13.595323 70-79
ATGGG 3626065 0.9727349 28.015554 15-19
AGCTG 3228535 0.9696591 5.1950855 80-89
CAGCG 3399475 0.96672124 6.2517486 160-169
ACTAA 1630715 0.951541 12.581732 240-247
CCCAA 2141425 0.9503091 10.646508 240-247
CGGAG 3700845 0.9400168 6.4100695 210-219
AGTTT 2501650 0.9383032 5.00066 220-229
TAGGC 3086285 0.92693585 5.7788687 160-169
CTGTA 2608015 0.9261953 5.0652313 230-239
AAGAG 2073745 0.9140527 12.031572 35-39
ACAAT 1564045 0.9126383 7.6714234 100-109
CTATA 1832705 0.90719986 5.1190133 90-99
TCGAA 2164385 0.90607893 17.003304 70-79
TGCAT 2550980 0.9059402 6.1532135 210-219
GAGTT 2854905 0.9055855 5.0701013 160-169
TTCTG 3001985 0.90440285 5.7593303 210-219
CCATT 2270155 0.90261525 5.414567 50-59
CCAGC 2792090 0.88894206 5.993894 80-89
CTTCC 2772105 0.88530636 5.508111 230-239
CATCA 1871310 0.8770653 5.675672 230-239
CGTGG 4019635 0.8661283 6.314408 50-59
TGAAC 2054355 0.860017 6.466745 190-199
CGGTC 3529460 0.85144895 5.877799 130-139
TGAAG 2269320 0.8485405 8.851398 35-39
GTATG 2618460 0.83058435 5.309973 220-229
GGTGG 4302670 0.8280928 19.34868 25-29
AGCGT 2752475 0.8266792 6.4844065 160-169
ATCAA 1381510 0.806127 12.040821 70-79
TCCAG 2382765 0.8012157 5.117976 240-247
TAACC 1702030 0.7977254 6.330755 80-89
GAGGC 3134840 0.7962512 12.1739235 30-34
AATCC 1697500 0.7956022 10.401929 240-247
CAATC 1683985 0.7892678 10.262116 240-247
ACGTC 2344115 0.7882195 9.608701 70-79
AAAGA 1282050 0.78765994 5.9728355 8
GGTGC 3640630 0.7844625 5.1423826 220-229
AAAGG 1764145 0.7775891 9.240457 10-14
AACCG 1953655 0.77438194 5.750996 190-199
AGCCA 1916795 0.7597715 6.268752 80-89
CTAAT 1534815 0.7597425 9.592423 240-247
ATGAT 1709210 0.75570303 6.9839745 240-247
TGAGA 2019555 0.75514877 5.572771 240-247
GGGCC 3696360 0.7541291 14.6093 15-19
GTCGA 2453010 0.73673767 12.4100895 70-79
AAGGT 1935245 0.7236237 10.112793 10-14
CGTCC 2626325 0.7093384 7.8829417 70-79
GTAGC 2359905 0.7087746 5.5186963 80-89
TGGGC 3251995 0.7007216 15.948974 15-19
GACCG 2400440 0.682622 14.88755 45-49
GGTCC 2818665 0.6799763 10.642497 50-59
GGAAG 2109170 0.66697496 5.916024 30-34
CATAC 1413215 0.6623605 7.8149257 70-79
TATGA 1495570 0.6612451 5.5669727 240-247
TAGGT 2081395 0.6602255 5.5542526 10-14
AGACC 1657975 0.6571815 5.1540284 240-247
AAAGC 1307845 0.6453968 9.21151 70-79
GAGGT 2345290 0.6291519 7.008722 10-14
ATAGG 1662575 0.6216674 5.436981 10-14
AGGAT 1653905 0.6184256 5.190031 10-14
AGAAG 1373960 0.6056057 5.1023335 9
CATCC 1567775 0.59021014 7.324791 240-247
GGGTC 2708055 0.5835165 5.9030447 70-79
AATGG 1557145 0.5822452 6.504854 10-14
CGATG 1890045 0.56765676 12.376439 15-19
AAATG 1083410 0.5646608 5.054165 9
GAAAA 907725 0.55768394 6.601156 8
TAAAA 754995 0.5484749 6.168822 8
GAAGG 1684645 0.532729 5.4588842 10-14
AAGAA 865675 0.53184944 5.745381 9
AAGGG 1659095 0.52464944 6.4397855 10-14
TGATC 1435555 0.5098146 5.39508 240-247
GGTCG 2322965 0.5005394 6.0665336 70-79
GATCC 1476705 0.49654886 5.4770412 240-247
AAGCA 1002870 0.49489748 6.0108156 70-79
ATAAG 891100 0.46443105 6.2166486 9
CGGTT 1767365 0.45029882 8.252558 10-14
ACGAT 1047885 0.43867728 5.567564 10-14
TAAGG 1166055 0.4360094 5.924049 10-14
TAAAG 791805 0.41267964 5.4957285 9
AAGCT 943450 0.39495754 8.015462 30-34
TGGGT 1732800 0.3943377 5.18742 10-14
GAAAG 866905 0.38210914 5.15677 9
Produced by FastQC (version 0.10.1)