FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_2_SL248348.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_2_SL248348.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13388758
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 15613695 8.843306 62.065456 110-119
ATACA 14426100 7.727688 37.281994 110-119
CACAG 20800185 7.563396 42.914333 110-119
TCCCT 24090590 6.972387 105.16451 30-34
CAGTA 17426650 6.6999536 39.80109 110-119
AGTAA 13524130 6.5036755 48.52552 110-119
GGAGA 21601750 6.3304744 96.458534 35-39
GTAAT 14694770 5.9735017 40.113495 110-119
GGTGA 23965900 5.936872 103.758224 45-49
CCCTG 23649910 5.811712 69.178 30-34
AATAC 10271565 5.502211 39.441227 110-119
GTAGT 18672215 5.447776 30.865498 80-89
AGACG 16500845 5.386475 103.653595 40-44
ACAGT 13830425 5.317328 36.240726 110-119
GTGAC 18188945 5.019062 86.04039 45-49
TAGTA 11912565 4.84252 39.805195 80-89
GAGAC 14698395 4.7980895 143.69661 40-44
CCCCA 14764505 4.781102 102.108154 60-69
CTCAG 15174180 4.6641393 34.73227 140-149
ACTCC 13483805 4.616681 119.555504 30-34
GGCCC 21501060 4.4861627 59.098988 60-69
ACCAG 12298175 4.471882 93.09606 45-49
GCACA 12071740 4.389545 34.767246 110-119
AGTAG 12626520 4.3580317 32.361195 80-89
CGTGT 18666755 4.354121 23.302103 120-129
GTGTC 18436225 4.3003488 23.62251 120-129
TGACC 13972430 4.2947536 146.55247 45-49
CACTC 12482640 4.2738953 97.68544 25-29
CTCCC 15108870 4.135783 68.38742 30-34
CCCAG 13820000 4.017594 87.708954 60-69
ATGCA 10384495 3.9924846 96.08124 25-29
GACCA 10851450 3.9458208 106.42308 45-49
TGAGG 15372295 3.8080504 70.35892 35-39
AGATA 7863775 3.7816436 20.687273 150-159
CCCTT 13012995 3.7662683 37.139797 20-24
AGGAG 12840890 3.763071 96.92992 35-39
ATGGT 12823430 3.7413435 25.990562 190-199
TACAC 8715995 3.732725 27.152342 110-119
CCACT 10842990 3.7124996 27.832037 240-247
GACGG 15779625 3.6970243 109.85426 40-44
ACGGT 13368690 3.68896 97.5874 40-44
CTGAG 13238530 3.6530437 106.227585 35-39
CAGGG 15443290 3.6182241 35.362255 50-59
TGTCC 13787980 3.5824776 20.618639 120-129
GAGGA 12143225 3.5586178 127.408585 35-39
TGCAC 11372045 3.4954643 115.01578 25-29
TGGTG 16526045 3.4605846 18.078232 190-199
GCCCC 14850165 3.4514186 73.304665 60-69
CTTGG 14514920 3.385684 21.371582 20-24
GGGTT 16130525 3.377762 41.617943 50-59
CCGTG 15278505 3.3705773 23.53295 120-129
AGGCT 12179650 3.360856 26.819239 30-34
TCTTG 12192695 3.3495839 18.391294 170-179
CTCTC 11552525 3.343574 14.275887 130-139
CCTGA 10877780 3.3435392 84.20731 30-34
GGATG 13372715 3.3127103 70.0752 20-24
CAGAT 8559715 3.2909193 18.542227 150-159
CTTCA 9061915 3.2805424 17.566484 200-209
TCAGG 11837190 3.2663577 21.26787 140-149
GCACT 10544110 3.240979 88.9962 25-29
CCAGG 12415030 3.2400713 39.965843 50-59
TCGCA 10365150 3.1859715 24.156895 100-109
TCTCA 8761080 3.1716359 22.11874 130-139
TGTCT 11464995 3.1496696 19.993925 180-189
CGCAC 10688685 3.1072936 24.633879 100-109
CCTGG 13988640 3.0860212 28.951572 50-59
ACAGA 6696260 3.045605 14.244507 140-149
CGGAT 10911085 3.010808 79.346306 20-24
GGTTG 14315310 2.9976528 73.36186 15-19
ACCAC 7372270 2.986084 20.17402 230-239
GCCCT 11920030 2.92922 20.04528 20-24
TTCCC 10110720 2.9262817 48.51758 50-59
GATGC 10511680 2.9005957 95.97859 25-29
TGGCC 13109015 2.891968 43.338932 60-69
TCTCT 9423980 2.8838763 15.520044 180-189
GGTTC 12262930 2.8603947 46.789177 50-59
AGGGT 11520630 2.8539093 49.666813 50-59
GATGG 11407665 2.825925 20.876156 15-19
GACTC 9128280 2.80579 18.724745 190-199
GTTGG 13337855 2.7929718 52.783295 15-19
TCAAA 5199930 2.7854683 57.73248 70-79
CGGCC 13330435 2.7813747 12.752578 120-129
TTCTT 8582475 2.7769184 20.813486 170-179
CGGTG 13962480 2.7652528 68.803215 40-44
GCCGT 12407015 2.7371008 22.217506 120-129
AGATG 7872560 2.717207 17.697918 180-189
TCACA 6157135 2.636864 11.429236 140-149
AGTCA 6857305 2.6364002 31.293692 70-79
CCAGT 8525480 2.6205053 35.344482 60-69
GTTCC 10042990 2.6094313 52.784046 50-59
TGGAG 10521430 2.6063855 18.222559 25-29
TGTTC 9435230 2.5920515 13.497207 140-149
TCCAT 7141345 2.585269 19.509586 70-79
ACAGC 7027165 2.5552287 11.618367 120-129
GGCTG 12819360 2.5388591 13.935942 30-34
AGGGA 8610940 2.5234685 8.927924 150-159
TCCTC 8687120 2.5142581 17.285166 130-139
GCGGA 10699230 2.5067337 87.45009 20-24
GAGAT 7201925 2.4857378 17.27658 180-189
TACCA 5679435 2.4322834 16.704222 230-239
GGCGG 14311165 2.4065108 42.256256 20-24
GTTCT 8759380 2.4063816 17.802408 160-169
CTGGA 8678765 2.394821 15.268338 180-189
AATAA 3554155 2.3813798 10.204463 110-119
ACTCT 6563495 2.376079 13.091168 200-209
CATTT 6174400 2.3633537 18.515144 150-159
CACGG 9043710 2.3602252 9.514409 120-129
TTGGG 11163635 2.337686 79.370605 15-19
TCATT 6097620 2.333965 17.159374 150-159
GTGAA 6740315 2.3264136 18.936764 190-199
TTCAT 6050455 2.3159118 16.681566 140-149
ACTAC 5405935 2.3151538 13.6074915 230-239
CAGCT 7444150 2.2881339 11.943037 150-159
GATAC 5947030 2.2864306 15.475609 150-159
TGTAG 7820410 2.2816703 7.77564 210-219
CTGGC 10330825 2.2790742 34.163292 60-69
TTCAC 6255440 2.2645583 19.268538 200-209
CTACT 6192365 2.2417247 8.193133 230-239
CAGAG 6825615 2.2281287 9.244477 140-149
ATTTG 6439385 2.2127237 16.352808 150-159
TTGTC 8054165 2.2126446 13.577039 170-179
CACTA 5155285 2.2078102 15.856895 240-247
TACAG 5601705 2.1536648 14.756311 160-169
GAATC 5544600 2.1317098 14.990429 190-199
TCAGC 6857610 2.107847 13.792119 140-149
ATGTA 5178075 2.1049147 8.763054 70-79
ATATA 3691365 2.0907204 11.233203 110-119
CTCTG 8046585 2.0907133 14.926114 180-189
CTCGC 8488255 2.085898 16.247862 100-109
TGAAT 5126980 2.0841444 14.479419 190-199
TGGGG 11721725 2.0840719 52.53898 15-19
GTCTC 8010645 2.0813751 12.33615 180-189
TTGGA 7132090 2.0808473 15.974357 170-179
ACACA 4101565 2.0779831 14.23073 110-119
TCTGC 7992970 2.0767827 12.314111 210-219
GTTCA 6367755 2.069478 15.552619 140-149
GCTGT 8862250 2.0671678 10.589831 140-149
GTCCT 7936050 2.0619936 14.685854 130-139
CAGGC 7869055 2.053664 15.776371 140-149
TAGTC 6280195 2.0410218 25.77919 70-79
AATCG 5295200 2.035824 13.655607 190-199
AATAT 3580815 2.028107 13.847802 110-119
TTGCA 6227745 2.0239758 14.802591 150-159
TCTCG 7721490 2.0062451 15.522156 100-109
TTTGC 7283640 2.0009654 12.53902 150-159
AGCTC 6498320 1.9974104 11.88993 140-149
TACTA 4403770 1.9940771 10.892127 230-239
CCTTC 6888575 1.9937165 14.4622555 200-209
GAGTC 7190595 1.9841746 12.811394 210-219
TGGAA 5730325 1.9778165 9.744533 170-179
GAACT 5131450 1.9728677 10.66116 160-169
TCGGC 8931485 1.970367 8.373935 130-139
ACGGC 7546925 1.9695946 14.583777 120-129
AACCA 3882495 1.9669951 14.920198 70-79
GTCAA 5103580 1.962153 39.830124 70-79
GATAT 4822245 1.9602679 8.101529 180-189
ATATG 4765310 1.9371234 8.345832 180-189
ATCTC 5346865 1.9356413 7.7213545 100-109
CAGCC 6620660 1.9246833 10.510702 120-129
AGTAT 4732740 1.9238837 12.9150715 220-229
CCAGA 5219495 1.8979207 29.412348 60-69
ACACG 5197505 1.8899249 13.311463 120-129
CATAG 4874385 1.8740352 8.242713 210-219
GGGGC 11041875 1.8567595 47.87837 15-19
GACGT 6712180 1.8521607 9.407003 170-179
CTGTT 6710585 1.8435357 13.258686 140-149
CGTAG 6627400 1.8287666 10.010668 210-219
GCAGA 5543535 1.8096111 15.18517 150-159
GGGCG 10751780 1.8079783 59.00944 20-24
GTTGT 7327210 1.8070852 6.7600675 210-219
ATTGT 5175665 1.7784797 8.066123 50-59
AGTCT 5462880 1.7753999 13.586819 210-219
AGAAC 3865810 1.7582546 11.352267 160-169
TGCAG 6324795 1.7452657 13.812357 150-159
CCTTG 6690310 1.7383174 24.229315 20-24
CACCA 4213855 1.7067913 13.334121 230-239
ATAGT 4131700 1.6795578 7.3601418 220-229
CAAAA 2612935 1.6558185 22.047636 70-79
GGTCA 5999585 1.655527 7.432909 130-139
GTCCC 6723445 1.6522146 21.920134 50-59
TCCCA 4822375 1.6511191 10.472309 240-247
AACTG 4282240 1.6463754 10.512604 160-169
GTCAC 5342190 1.6420472 8.329959 140-149
TCTGG 7025105 1.6386439 12.3525915 180-189
TGACT 5026370 1.6335372 11.25663 190-199
CTCCA 4770860 1.633481 7.340321 150-159
GAGAA 3991290 1.629684 10.5007305 160-169
AATAG 3377945 1.624434 9.966332 240-247
GAACC 4465610 1.6237919 12.327252 70-79
TGGTT 6556665 1.6170481 17.982542 10-14
TTGGT 6498685 1.6027488 25.496288 25-29
GTCTG 6863575 1.6009661 9.375356 210-219
ACTGA 4157965 1.5985961 8.644066 180-189
TCAGA 4129230 1.5875485 9.773319 130-139
TTGTA 4557180 1.565954 8.608194 210-219
TGTGC 6713370 1.5659298 8.076247 220-229
GTGCT 6686700 1.5597087 8.0776 220-229
ATAGA 3242765 1.5594268 10.035448 240-247
TAGTT 4525570 1.555092 9.497113 220-229
GCTCC 6314095 1.5516213 5.9517684 150-159
GGCCG 8282820 1.551466 13.064819 120-129
ACCAT 3618935 1.5498506 12.410101 45-49
TTCAG 4759860 1.546923 9.165065 150-159
CCACC 4774525 1.546106 9.873466 230-239
CAAAG 3386260 1.5401449 28.623419 70-79
GCTCT 5899285 1.5327886 10.474371 130-139
CTGGT 6557715 1.5296224 6.7543817 160-169
CAGAC 4205540 1.5292251 17.769138 60-69
GGACG 6519050 1.527355 7.004442 170-179
CCTCG 6208065 1.5255655 10.119965 130-139
CCCAC 4710530 1.525383 10.597449 240-247
CTGTG 6522105 1.5213164 5.0191708 210-219
TGCTC 5854205 1.5210756 5.054177 150-159
GCTCA 4923110 1.5132331 10.698137 140-149
AAAAA 1876680 1.4875307 8.8657255 8
GCTGA 5295560 1.4612583 22.856075 30-34
AATTG 3577005 1.4540713 9.819335 170-179
AAATA 2160670 1.4477072 5.065781 9
TATAC 3163415 1.4324301 7.1682057 110-119
CGACT 4623370 1.421101 8.302534 190-199
CATGT 4367180 1.4193048 8.613263 70-79
TGATA 3471025 1.41099 7.6193976 180-189
GCGTA 5105340 1.4087689 10.618134 210-219
ACGGA 4293300 1.401489 9.870756 200-209
ACCGT 4536245 1.3943211 21.04394 45-49
CACTG 4533480 1.3934712 7.0958405 230-239
TCGAC 4524540 1.3907232 8.978009 190-199
CTCTT 4475400 1.3695382 7.8483424 200-209
GAATT 3355380 1.3639796 10.84424 170-179
TGGAC 4920195 1.3576798 9.899085 170-179
GGGAG 6440305 1.3545995 6.406742 150-159
GAGGG 6423870 1.3511426 7.9931335 200-209
GTGGA 5401340 1.3380288 23.069569 25-29
CCATC 3887605 1.3310659 10.409886 240-247
CCTCA 3877115 1.3274741 7.1518536 130-139
TATGG 4531700 1.3221616 6.0542364 180-189
GTCCA 4285760 1.3173287 18.266232 70-79
CTCGA 4277785 1.3148774 8.053857 190-199
GTGTT 5323745 1.3129773 7.9219375 160-169
AGCCG 5002645 1.305589 9.359043 120-129
GGAGT 5261995 1.3035101 7.411441 210-219
ACTTC 3594310 1.3011916 6.569345 220-229
GGCTC 5871685 1.2953471 8.807817 130-139
CCATG 4201640 1.2914722 8.715077 70-79
CTGAT 3968450 1.2897202 7.1947207 180-189
GGAGG 6076175 1.2780114 17.41907 30-34
TCCAC 3732365 1.2779137 9.2527685 240-247
ATCGG 4627890 1.2770213 8.184388 190-199
CTTGA 3916760 1.2729211 8.133709 200-209
TTGGC 5451225 1.2715276 8.79592 60-69
CTGCT 4890810 1.2707605 5.5928464 90-99
GGAAT 3663690 1.2645192 9.118845 170-179
ACGTG 4533355 1.2509353 8.990161 170-179
ATAAC 2335155 1.250882 7.5217295 80-89
GTGGT 5973530 1.2508683 11.940219 50-59
AGATC 3246450 1.2481495 6.658132 130-139
AAGTA 2586180 1.2436789 15.880449 70-79
ACTAT 2737210 1.2394398 6.22115 230-239
ATAAA 1844555 1.2359016 5.810315 7
TGGTC 5264275 1.2279205 13.475284 50-59
ACTCG 3969890 1.2202386 8.291679 190-199
AAAGT 2494560 1.1996193 21.039192 70-79
TCACG 3894965 1.1972086 9.925948 200-209
ATACC 2781695 1.1912929 8.387992 70-79
ACTGG 4314735 1.1906092 6.998299 160-169
TTGAG 4076405 1.1893255 7.8431273 200-209
TGCCC 4829210 1.1867267 7.5229173 60-69
GATCT 3636615 1.1818758 5.2314887 130-139
CCATA 2752460 1.1787727 11.50305 70-79
TTCCA 3226380 1.1679956 7.4595556 230-239
GCATA 3036360 1.167377 7.6809435 210-219
AGCTT 3566285 1.159019 6.151609 150-159
CGGCT 5125965 1.1308346 8.295832 130-139
AGTTC 3479245 1.1307316 5.603739 160-169
TATCA 2493445 1.1290601 7.1949825 70-79
CTCGG 5114785 1.1283683 8.747533 130-139
ACTGT 3464065 1.1257983 5.3471704 230-239
AAAAG 1976095 1.1241926 15.098391 70-79
TATGT 3251720 1.1173671 8.030254 220-229
AGAGC 3411445 1.1136196 6.9695563 140-149
CAATA 2066385 1.1069092 6.599751 100-109
AGGTT 3793115 1.1066731 23.3686 10-14
TCTAC 3047750 1.103329 7.1621776 180-189
CTACC 3209380 1.0988504 8.945425 230-239
GTCTA 3348330 1.0881851 7.3222957 180-189
ATGGG 4377040 1.0842874 32.868057 15-19
GAAGA 2647270 1.080907 18.791073 35-39
CTGCG 4879595 1.0764831 7.2664647 210-219
TCTGT 3915905 1.0757796 6.0939546 210-219
AGAGA 2610420 1.0658607 14.030638 35-39
TACTG 3275875 1.0646378 6.3571916 180-189
ATCCA 2477865 1.0611742 14.650936 240-247
GCTTC 4068835 1.0571898 5.43171 150-159
GAGCT 3826550 1.0558993 6.2024117 140-149
ATATC 2303205 1.0429174 7.627926 70-79
ATACG 2702415 1.0389866 5.6204143 120-129
TGCGT 4428510 1.0329739 8.422324 210-219
CTGCC 4145445 1.0186988 5.5752826 200-209
CTGAA 2634055 1.0127046 15.319964 35-39
TATAA 1775215 1.0054487 6.642501 240-247
AACTT 2218820 1.0047069 5.5809426 200-209
CGGAG 4283195 1.0035142 6.9445534 210-219
TCCCC 3659495 1.0017214 5.3502035 80-89
GCGGT 5036295 0.99743235 5.5963573 130-139
TAGAT 2443635 0.99335045 7.6367774 240-247
CAGCG 3804770 0.99296796 6.700768 160-169
GGGGT 5573985 0.99103034 7.577665 10-14
CATGG 3574360 0.9863099 5.1426506 190-199
AAAAT 1469885 0.98486274 5.4080844 8
CGAAC 2686370 0.9768221 12.911189 70-79
ATGTG 3341490 0.97490776 5.03922 220-229
ACTAA 1819225 0.97451186 13.136319 240-247
ACCCA 2397265 0.9709947 12.945329 240-247
CGTGG 4833345 0.9572384 8.267932 50-59
GAGTT 3271080 0.9543651 5.4019938 160-169
AGCTG 3446785 0.9511067 5.0803423 90-99
CCATT 2616700 0.9472827 6.0783696 50-59
ACAAT 1758665 0.94207144 7.566281 100-109
TGAAG 2714960 0.9370661 10.236415 35-39
CTTCT 3050600 0.9335284 5.120461 210-219
CCCAA 2289640 0.9274019 9.481018 240-247
TGTAA 2279660 0.92669374 6.5890527 230-239
AAGAG 2242290 0.9155497 13.934206 35-39
ACGTC 2969070 0.912613 12.497576 70-79
TAGGC 3276760 0.90419 5.510867 160-169
GGTGG 5009905 0.8907393 22.842783 25-29
TGCAT 2729780 0.88716054 5.8722835 210-219
TCGAA 2305885 0.88653433 16.515182 70-79
GAGGC 3767725 0.8827441 14.802634 30-34
AGCGT 3158710 0.87161523 6.8746386 160-169
CATCA 2033735 0.8709704 5.586531 230-239
TGAAC 2252465 0.8659962 6.877913 190-199
ATCAA 1605545 0.8600489 13.632682 70-79
CCAAT 2001615 0.8572147 10.325655 240-247
GTATG 2905940 0.8478325 5.6445117 220-229
GGGCC 4464325 0.83621854 17.32708 15-19
AACCG 2298550 0.83580226 6.1235003 190-199
CCAGC 2869795 0.83427423 5.651935 80-89
CGTCC 3388825 0.8327675 10.188941 70-79
CGGTC 3765785 0.83076644 5.3557053 130-139
CTTCC 2847410 0.8241078 5.290479 230-239
TTCTG 2994855 0.82274824 5.1031275 210-219
GACCG 3095660 0.80790454 19.345844 45-49
GGTCC 3620165 0.7986413 13.6323805 50-59
TAACC 1844090 0.7897527 6.008565 80-89
TGGGC 3967010 0.7856617 18.795153 15-19
CTAAT 1711170 0.77483726 10.030646 240-247
AAAGG 1849925 0.75534296 9.061042 10-14
AAAGA 1321895 0.7520208 5.7239428 8
CATAC 1751965 0.75029916 9.9747925 70-79
TGAGA 2172485 0.74983126 5.9918947 240-247
AGCCA 2031775 0.7387971 5.861011 80-89
AATCC 1701180 0.7285499 9.094706 240-247
GTAGC 2578455 0.71149963 5.5991316 80-89
ATGAT 1745795 0.70967484 6.515992 240-247
CCTTT 2309390 0.70670724 5.127644 50-59
GTCGA 2556745 0.70550895 11.576317 70-79
AAAGC 1531675 0.6966391 10.261946 70-79
AAGGT 2016945 0.6961468 10.070629 10-14
CATTG 2065695 0.6713373 5.5842123 50-59
CGATG 2402780 0.66302377 14.472306 15-19
TAGGT 2265195 0.6608897 5.45553 10-14
TATGA 1585070 0.64433926 5.636495 240-247
AGGAT 1862825 0.6429523 6.202773 10-14
CCGTA 2077650 0.63861424 5.3873277 80-89
CAATC 1465410 0.6275787 8.795613 240-247
GAGGT 2532315 0.6273092 6.8579426 10-14
GGAAG 2122960 0.6221414 6.2625413 30-34
AGAAG 1518480 0.62001073 5.6163096 9
AGACC 1688075 0.61382043 5.2009645 240-247
ATAGG 1763340 0.60861516 5.2972836 10-14
AATGG 1683405 0.58102566 6.6567116 10-14
TAAAA 844870 0.5660857 5.85862 8
CATCC 1638490 0.56099784 6.892548 240-247
GGGTC 2790450 0.5526454 5.5382967 70-79
AAATG 1134205 0.54543257 5.3293037 9
AAGAA 956820 0.5443311 5.366454 9
AAGCA 1169545 0.5319345 6.883831 70-79
GAAGG 1782595 0.52239615 5.5968122 10-14
AAGGG 1755095 0.5143372 6.3233533 10-14
GAAAA 884795 0.50335634 5.859658 8
TTGCC 1848355 0.48025095 5.3151937 60-69
GGTCG 2419385 0.47915635 5.6227164 70-79
ACGAT 1239980 0.47673014 6.6317363 10-14
ATAAG 983015 0.47272617 5.7498507 9
TACCG 1432675 0.44036612 5.289357 70-79
CGGTT 1863095 0.43457693 7.982812 10-14
TAAGG 1252785 0.4323976 5.993283 10-14
GAAGC 1310790 0.42788947 5.113108 30-34
AAGCT 1071400 0.41191682 8.529506 30-34
TAAAG 840510 0.40419635 5.756385 9
GAAAG 914010 0.37319952 5.1074324 9
Produced by FastQC (version 0.10.1)