FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_15_SL248344.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_15_SL248344.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9841072
Filtered Sequences 0
Sequence length 251
%GC 54

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 11155275 8.186529 57.395985 110-119
CACAG 14846315 7.191616 40.596348 110-119
ATACA 10222290 7.1102314 34.80688 110-119
TCCCT 17246845 6.990318 105.28314 30-34
CAGTA 12466020 6.3711777 37.672436 110-119
AGTAA 9659840 6.0173583 44.85892 110-119
GGAGA 15433840 5.9962897 90.461044 35-39
CCCTG 17130345 5.893452 68.882324 30-34
GGTGA 17185035 5.7796164 100.84166 45-49
GTAAT 10528430 5.677269 37.923244 110-119
GTAGT 13059045 5.174205 28.154173 80-89
AATAC 7388625 5.139243 36.787754 110-119
AGACG 11718175 5.0835633 97.72962 40-44
ACAGT 9747145 4.981605 34.259624 110-119
GTGAC 13073900 4.9096813 83.02286 45-49
GAGAC 10816075 4.692215 136.5152 40-44
CCCCA 10503620 4.661249 99.59056 60-69
ACTCC 9939280 4.6537423 118.25956 30-34
CTCAG 10852545 4.550712 33.819008 140-149
GGCCC 15248605 4.4529743 59.19575 60-69
ACCAG 9160605 4.4374347 89.24026 45-49
TAGTA 8164635 4.4026346 35.226418 80-89
AAAAA 4412460 4.321361 27.886944 130-139
GCACA 8907295 4.314731 33.016644 110-119
GTGTC 13243185 4.3050733 23.47592 120-129
CGTGT 13194105 4.2891183 22.980812 120-129
CACTC 9013645 4.220344 95.54702 25-29
TGACC 10025035 4.203719 141.1599 45-49
CTCCC 10784790 4.1430006 68.87562 30-34
AGTAG 8885855 4.067162 28.62543 80-89
CCCAG 9843040 3.9119475 85.1434 60-69
GACCA 7992525 3.871612 101.41826 45-49
ATGCA 7553760 3.8606024 91.860664 25-29
TGAGG 11100230 3.7331936 67.89795 35-39
CCCTT 8978735 3.6391706 36.225277 20-24
ATGGT 9115065 3.611536 25.132738 190-199
CCACT 7710210 3.610053 26.545143 240-247
CAGGG 11315045 3.6067905 35.114105 50-59
GACGG 11308360 3.6046593 105.84069 40-44
AGGAG 9267740 3.600663 90.667404 35-39
ACGGT 9564575 3.5918143 93.8837 40-44
TGTCC 9847550 3.5745046 20.283724 120-129
CTGAG 9509540 3.571147 103.60085 35-39
TACAC 6232315 3.5566478 25.755104 110-119
TGCAC 8284910 3.4740458 112.55152 25-29
AGATA 5548780 3.4564753 18.349865 150-159
GCCCC 10540285 3.436941 73.173294 60-69
GGGTT 11801750 3.4358554 42.143158 50-59
TGGTG 11785005 3.4309804 17.86359 190-199
GAGGA 8727495 3.3907692 119.45682 35-39
CTTGG 10222280 3.3230422 20.742264 20-24
CCGTG 10766555 3.3172703 23.32441 120-129
CTTCA 6703635 3.3116298 16.606289 200-209
TCTTG 8612025 3.2982 18.00777 170-179
CCTGA 7843730 3.2890494 81.71302 30-34
GGATG 9722760 3.269927 68.01646 20-24
CTCTC 8056695 3.2654586 13.590622 130-139
CCAGG 9088250 3.2347775 39.775166 50-59
AGGCT 8606385 3.231982 24.708963 30-34
GCACT 7640995 3.2040384 86.8048 25-29
CAGAT 6180905 3.1589587 17.297127 150-159
TCAGG 8409345 3.1579869 20.419737 140-149
CCTGG 10218795 3.1485004 29.172832 50-59
TGTCT 8140875 3.1177607 19.678392 180-189
TCGCA 7324870 3.07148 23.268288 100-109
TCTCA 6176115 3.0510323 20.918556 130-139
GGTTG 10371740 3.019535 73.800934 15-19
CGCAC 7546355 2.9991698 23.824503 100-109
TTCCC 7357020 2.9818738 49.292915 50-59
CGGAT 7886175 2.9615192 77.165985 20-24
ACAGA 4948500 2.921632 13.503747 140-149
TGGCC 9477030 2.9199562 43.63829 60-69
GGTTC 8975310 2.917679 47.5372 50-59
GCCCT 8371170 2.8799827 19.64161 20-24
GATGC 7634020 2.8668263 94.50235 25-29
AGGGT 8491375 2.8557918 48.86329 50-59
TCTCT 6657555 2.846991 15.1959505 180-189
ACCAC 5207520 2.816686 18.632555 230-239
GTTGG 9666060 2.8140898 52.792564 15-19
GCCGT 9060730 2.7916906 22.064358 120-129
CGGTG 10056470 2.7749171 67.70814 40-44
TCACA 4859280 2.7730863 11.188062 140-149
GACTC 6609675 2.7715828 18.534834 190-199
GATGG 8176610 2.7499309 19.859358 15-19
AGTCA 5376340 2.7477586 30.846857 70-79
CCAGT 6499045 2.7251933 36.1882 60-69
TTCTT 6033280 2.7221296 20.248966 170-179
CGGCC 9307300 2.7179644 12.56903 120-129
TCAAA 3841450 2.671965 54.738663 70-79
GTTCC 7284900 2.6443033 53.761517 50-59
AGATG 5642375 2.5825825 16.501505 180-189
GGCTG 9150345 2.5248868 13.187232 30-34
TCCAT 5076900 2.5080142 17.645432 70-79
TGTTC 6517000 2.4958553 12.922989 140-149
TGGAG 7414965 2.493777 16.966953 25-29
TCCTC 6148330 2.4919794 17.04771 130-139
GCGGA 7750865 2.47067 85.33595 20-24
AGGGA 6326060 2.457774 8.634657 150-159
GGCGG 10387155 2.432862 42.31783 20-24
ACAGC 4927625 2.386962 10.860271 120-129
GAGAT 5178290 2.3701653 16.057095 180-189
TTGGG 8119420 2.363815 79.183235 15-19
GTTCT 6145180 2.3534572 17.297554 160-169
ACTCT 4760365 2.3516443 13.120694 200-209
CTGGC 7569230 2.3321462 34.896847 60-69
CTGGA 6121880 2.2989686 14.392386 180-189
AATAA 2710705 2.2980628 9.380917 110-119
TACCA 3989750 2.2768643 15.490115 230-239
CACGG 6391850 2.275049 9.323659 120-129
CAGCT 5407980 2.2676857 11.861824 150-159
TGTAG 5653095 2.2398474 7.6805425 210-219
CATTT 4295420 2.2388294 17.574816 150-159
TCATT 4281350 2.231496 16.267607 150-159
TTCAC 4516060 2.2309566 18.275763 200-209
TCAGC 5280815 2.2143626 13.855116 140-149
TTCAT 4219120 2.199061 15.613158 140-149
TCTGC 5989625 2.1741393 11.945265 210-219
TTGTC 5676725 2.17405 13.244617 170-179
GTGAA 4733425 2.1665454 17.437315 190-199
CAGAG 4972195 2.1570313 8.945189 140-149
ACTAC 3740035 2.1343572 11.840918 230-239
CTACT 4317630 2.132931 7.2706 230-239
GTCAA 4146130 2.1190186 40.90704 70-79
TGGGG 8527660 2.1073406 52.292274 15-19
GATAC 4119200 2.1052551 13.922362 150-159
ATTTG 4503685 2.1022472 15.407188 150-159
CACTA 3661965 2.0898044 14.530351 240-247
TCTCG 5748600 2.0866508 16.761202 100-109
GTCTC 5736995 2.0824382 12.0318365 180-189
CTCTG 5699000 2.0686467 14.562217 180-189
CTCGC 6006240 2.066362 16.070442 100-109
ATCTC 4167210 2.0586228 9.271627 100-109
GAACT 4024750 2.0569832 10.383139 160-169
GTCCT 5657520 2.05359 14.41043 130-139
GCTGT 6309185 2.0509796 10.1156 140-149
ACACA 3108870 2.0495293 13.203813 110-119
TTGGA 5170830 2.0487664 15.3767185 170-179
TAGTC 4581240 2.0268183 25.71487 70-79
GAATC 3940210 2.0137763 14.075579 190-199
GTTCA 4542640 2.0097408 14.7567 140-149
GATAT 3724790 2.008527 7.752677 180-189
TACAG 3902990 1.9947537 13.331664 160-169
ATATA 2714490 1.9920845 9.95414 110-119
CAGGC 5575855 1.9846125 15.058126 140-149
CCTTC 4862845 1.9709594 13.988901 200-209
AGCTC 4697975 1.9699647 11.6830845 140-149
ATGTA 3647675 1.9669441 7.717774 70-79
TTTGC 5135365 1.9667221 12.280032 150-159
TTGCA 4443165 1.9657315 14.188282 150-159
TGAAT 3643170 1.9645149 13.463331 190-199
ACACG 4044110 1.9589838 12.766601 120-129
AATCG 3795625 1.9398812 12.999706 190-199
AATAT 2624730 1.9262123 12.468775 110-119
GAGTC 5100810 1.9155225 12.104529 210-219
TCGGC 6183720 1.9052584 7.8832393 130-139
TGGAA 4158695 1.9034846 8.961094 170-179
ACGGC 5338390 1.9000916 14.06324 120-129
AACCA 2854500 1.8818352 14.04495 70-79
GGGGC 8006540 1.8752784 47.71035 15-19
CAGCC 4684425 1.8617446 10.046602 120-129
ATATG 3425430 1.8471022 7.9299273 180-189
TACTA 3052530 1.8379573 9.529652 230-239
GGGCG 7789605 1.8244681 59.482372 20-24
CATAG 3562355 1.8206608 7.916041 210-219
CCAGA 3740375 1.811853 26.439539 60-69
CTGTT 4687880 1.795346 12.725686 140-149
GTTGT 5217880 1.7896421 6.825954 210-219
CTCCA 3789005 1.7740773 7.6785645 150-159
GACGT 4709780 1.768678 9.309585 170-179
AGTAT 3278035 1.7676222 11.39361 220-229
CGTAG 4689135 1.7609253 9.317456 210-219
GTCAC 4173635 1.7500974 8.277099 130-139
CCTTG 4755420 1.726142 23.58876 20-24
ATTGT 3684185 1.7197177 7.503455 50-59
TGCAG 4578065 1.7192147 13.39605 150-159
GCAGA 3957655 1.7169048 14.129243 150-159
CACCA 3162780 1.7107104 12.78921 230-239
AGTCT 3825315 1.6923841 12.658952 210-219
GTCTG 5197595 1.6896256 8.9283285 210-219
AGAAC 2854245 1.6851679 10.808674 160-169
TTCAG 3773865 1.669622 9.0601635 150-159
CAAAA 2052290 1.649052 19.83203 70-79
TGGTT 4769515 1.6358607 17.773752 10-14
GGTCA 4341430 1.6303504 7.295599 130-139
ATAGT 3013550 1.6250033 7.0033903 220-229
TGACT 3665150 1.6215246 11.173974 190-199
TCTGG 4977410 1.6180483 11.978529 180-189
TCCCA 3443950 1.6125166 10.242224 240-247
TTGGT 4679380 1.604946 25.240576 25-29
GTCCC 4640255 1.5964141 20.666079 50-59
TCAGA 3120260 1.5947134 9.541604 130-139
AACTG 3108640 1.5887746 10.171079 160-169
GCTCC 4586870 1.5780479 6.258047 150-159
TGCTC 4328715 1.571255 5.3090878 150-159
TGTGC 4818640 1.5664357 7.941511 220-229
CTGTG 4818585 1.5664178 5.17824 210-219
GAGAA 2957610 1.5638434 9.896919 160-169
CATGT 3530235 1.5618359 8.834034 70-79
AATAG 2494940 1.5541611 8.906623 240-247
GTGCT 4776570 1.5527596 7.7391167 220-229
TTGTA 3322295 1.5507934 8.617565 210-219
CTGGT 4769135 1.5503427 6.825302 160-169
ACTGA 3027255 1.5471802 8.244448 180-189
GGCCG 5907010 1.5448567 12.984578 120-129
TAGTT 3294290 1.5377213 9.391443 220-229
ATAGA 2460580 1.5327574 9.488512 240-247
GAACC 3141530 1.5217702 11.864304 70-79
CAAAG 2575550 1.5206243 27.616997 70-79
GGACG 4698265 1.4976217 6.959706 170-179
GCTCA 3567660 1.4959987 10.531649 140-149
GCTCT 4087675 1.4837614 9.958996 130-139
CCACC 3337310 1.4810166 9.398267 230-239
CCTCG 4300240 1.4794366 9.648052 130-139
CAGAC 3052305 1.4785494 15.660033 60-69
ACCAT 2586000 1.4757744 11.2911825 45-49
CCCAC 3324590 1.4753717 10.1081295 240-247
GCTGA 3815325 1.4327807 21.390759 30-34
CGACT 3376590 1.4158789 8.317477 190-199
CTGCT 3881245 1.4088305 5.697452 90-99
CACTG 3337550 1.3995085 7.036883 230-239
CTCTT 3244360 1.3873956 7.9628086 200-209
TCGAC 3291705 1.3802848 8.960195 190-199
TATAC 2286005 1.3764251 6.292355 110-119
CTTGA 3106500 1.3743684 8.036453 200-209
GAGGG 4813710 1.3741839 7.903145 200-209
AATTG 2546960 1.3734028 8.908496 170-179
GCGTA 3646375 1.3693342 9.813261 210-219
AGATC 2678435 1.3689039 6.645869 130-139
TGGAC 3633780 1.3646045 9.976208 170-179
GGGAG 4765800 1.3605069 6.2934756 150-159
TGATA 2509165 1.3530227 7.1552725 180-189
CCTCA 2876985 1.3470539 7.2957535 130-139
GTGGA 3968800 1.3347741 21.808697 25-29
ATCGG 3541900 1.3301004 7.846433 190-199
GGAAT 2883185 1.3196683 8.382987 170-179
CCATC 2811065 1.316189 10.277498 240-247
ACGGA 3019545 1.3099351 8.885863 200-209
GTAGG 3887735 1.3075105 5.0244765 160-169
ACTTC 2625885 1.2972004 6.544766 220-229
ACCGT 3085990 1.2940238 18.804777 45-49
CTCGA 3078345 1.2908181 8.011832 190-199
CTGAT 2907380 1.2862743 7.04903 180-189
GTGTT 3745655 1.2846946 7.4530997 160-169
GAATT 2373900 1.2800834 9.96739 170-179
TATGG 3217430 1.2747976 5.8865743 180-189
CCATG 3039175 1.2743932 7.950987 70-79
TCCAC 2720020 1.2735603 9.150637 240-247
GGAGT 3769160 1.2676318 6.9626093 210-219
AGCCG 3542625 1.2609252 8.992222 120-129
GGAGG 4405135 1.2575468 16.571884 30-34
GGCTC 4041690 1.2452803 8.320131 130-139
GTCCA 2968150 1.2446109 16.52762 70-79
GTGGT 4229565 1.2313576 11.020582 50-59
ACGTG 3268620 1.2274748 8.881183 170-179
ACTAT 2025355 1.2194855 5.9224606 230-239
ATAAC 1752770 1.2191594 7.2352433 80-89
ATAAA 1437665 1.2188137 5.090317 7
TTGGC 3738475 1.2152973 8.307501 60-69
TGGTC 3710705 1.20627 12.45214 50-59
ACTCG 2869165 1.2031043 8.161107 190-199
AAGTA 1926990 1.2003707 14.711211 70-79
GATCT 2695355 1.1924707 5.2485476 130-139
AAAGT 1908160 1.1886412 20.218187 70-79
AGAGC 2730825 1.1846828 6.6504464 140-149
GAAGA 2231860 1.1801013 18.475468 35-39
ACTGG 3116980 1.170529 6.920104 160-169
TGCCC 3398625 1.1692488 7.055878 60-69
TTGAG 2950505 1.1690376 7.6987085 200-209
TTCCA 2356865 1.1643033 7.4329357 230-239
ATATC 1923680 1.1582658 7.123862 70-79
AAAAG 1609315 1.1580762 13.300984 70-79
GCATA 2261125 1.1556238 7.581118 210-219
TCACG 2733065 1.1460346 9.233579 200-209
ATACC 1995795 1.1389571 7.323348 70-79
ACTGT 2553700 1.1298002 5.3850527 230-239
AGCTT 2546080 1.126429 5.949833 150-159
CTGAA 2197125 1.1229144 14.331269 35-39
TATCA 1854200 1.1164315 6.6638575 70-79
TCTGT 2902005 1.1113986 6.3527536 210-219
AGTTC 2494910 1.1037905 5.418623 160-169
CAATA 1578890 1.0982151 6.2093725 100-109
CCATA 1919930 1.0956626 10.166207 70-79
AGGTT 2759465 1.0933444 22.574982 10-14
CGGCT 3529915 1.0875978 7.818636 130-139
CTCGG 3528605 1.0871942 8.3277025 130-139
TCTAC 2185200 1.0794998 6.9618278 180-189
CTTCT 2519390 1.0773746 5.404864 210-219
GTCTA 2410025 1.0662359 7.1034408 180-189
TATGT 2275910 1.0623579 7.2215314 220-229
ATGGG 3150800 1.0596669 31.12402 15-19
CTGCG 3434220 1.0581133 6.7794466 210-219
GCTTC 2905260 1.0545634 5.3688865 150-159
AGAGA 1994170 1.0544221 12.656778 35-39
TACTG 2369655 1.0483756 6.1034846 180-189
ATCCA 1824815 1.0413826 14.198483 240-247
CTGCC 3026345 1.0411713 5.763798 200-209
GTCCG 3357190 1.0343797 5.0754166 130-139
AAGAG 1951790 1.0320137 13.629934 35-39
GAGCT 2745030 1.0308495 5.9549856 140-149
GGGGT 4151400 1.0258868 7.840912 10-14
GCGGT 3704280 1.022135 5.6726604 130-139
CTACC 2182325 1.0218022 8.194919 230-239
TAGAT 1886330 1.01717 7.4122124 240-247
TGCGT 3079905 1.0012105 8.0286 210-219
CATGG 2646560 0.99387074 5.1509504 190-199
AACTT 1647255 0.99182785 5.5210676 210-219
CAGCG 2758325 0.98176986 6.1672835 160-169
TCCCC 2548830 0.97913855 5.0515842 80-89
ATACG 1906100 0.9741762 5.038783 120-129
ACGTC 2318590 0.9722361 11.381612 70-79
CGGAG 3014450 0.9608878 6.4991336 210-219
TATAA 1308160 0.96002024 6.1912565 240-247
TGAAC 1873220 0.95737183 6.316586 190-199
TTCTG 2491250 0.9540892 5.4084997 210-219
CGTGG 3420840 0.94392455 7.6559324 50-59
ACAAT 1352870 0.94100434 7.179149 100-109
CGAAC 1932240 0.93598515 12.434944 70-79
ACCCA 1714410 0.92730415 12.157129 240-247
CCATT 1863030 0.92034626 5.754496 50-59
GAGTT 2320615 0.91946507 5.1841574 160-169
TGTAA 1700745 0.9170965 6.3128986 230-239
TAGGC 2440820 0.9166086 5.6165705 160-169
GTATG 2281830 0.9040979 5.1166816 220-229
GGTGG 3652990 0.9027206 22.11788 25-29
ACTAA 1295190 0.90088433 11.584512 240-247
CCCAA 1659105 0.89739025 9.17235 240-247
TGCAT 2005165 0.887119 5.8535614 210-219
CATCA 1546850 0.8827539 5.580881 230-239
TGAAG 1906960 0.8728384 9.449555 35-39
GAGGC 2726480 0.8690943 13.788978 30-34
TCGAA 1677415 0.85729927 15.809506 70-79
AAAGA 1189155 0.85572565 5.454207 8
AGCGT 2274360 0.8540973 6.311071 160-169
CCAGC 2142640 0.8515555 5.457615 80-89
GACCG 2387125 0.84964854 17.4669 45-49
CGGTC 2716040 0.8368358 5.3997283 130-139
GGGCC 3198465 0.8364925 16.882097 15-19
CCAAT 1462205 0.8344489 9.978742 240-247
CTTCC 2052805 0.83202237 5.3508763 230-239
ATCAA 1195135 0.83128995 12.197396 70-79
CAATC 1446375 0.825415 8.606342 240-247
CGTCC 2337580 0.80421144 9.518421 70-79
TGGGC 2846000 0.7853068 18.248976 15-19
TAACC 1374070 0.7841521 5.928949 80-89
AACCG 1616765 0.7831677 5.534657 190-199
TGAGA 1684440 0.7709884 5.277473 240-247
GGTCC 2501835 0.77083737 12.686426 50-59
GAAAA 1046565 0.7531166 5.493343 8
GGAAG 1926615 0.74852026 6.515269 30-34
AAAGG 1392440 0.736256 8.418342 10-14
AGCCA 1500595 0.7268944 5.4964814 80-89
CTAAT 1201190 0.7232479 9.144673 240-247
CATAC 1264870 0.721834 8.7574625 70-79
GTCGA 1891545 0.71033764 11.509686 70-79
ATGAT 1316240 0.70975906 6.4918666 240-247
AATCC 1238665 0.7068794 8.860507 240-247
GTAGC 1864475 0.70017195 5.456301 80-89
AAGGT 1494795 0.68418556 9.510345 10-14
AAAGC 1136075 0.67074734 9.256335 70-79
CATTG 1487305 0.658009 5.2635555 50-59
TAGGT 1636865 0.64855236 5.3636937 10-14
AGGAT 1401025 0.64126587 5.9623537 10-14
AGAAG 1195535 0.632142 5.0740213 9
GAGGT 1871200 0.629316 6.6334066 10-14
TATGA 1154265 0.62241685 5.3978734 240-247
CGATG 1641890 0.61658394 13.825171 15-19
TAAAA 723540 0.6133977 5.7874603 8
ATAGG 1327920 0.60780483 5.1357064 10-14
CCGTA 1436235 0.6022451 5.018913 80-89
GGGTC 2176465 0.60055965 5.6149664 70-79
TCAAT 987220 0.59441453 5.6480856 70-79
AATGG 1258730 0.57613575 6.421866 10-14
AAATG 916435 0.5708705 5.0236278 9
CATCC 1190540 0.55743134 6.7761316 240-247
GAAGG 1407145 0.546698 5.398328 10-14
AAGCA 900830 0.5318569 6.2150216 70-79
AAGGG 1323565 0.51422584 6.0962605 10-14
GGTCG 1787710 0.4932891 5.720592 70-79
TGATC 1096780 0.48523408 5.08384 240-247
ATAAG 755895 0.47086608 5.6299405 9
GATCC 1120760 0.46995944 5.113378 240-247
ACGAT 905495 0.46278358 6.018626 10-14
CGGTT 1368945 0.44501442 7.937287 10-14
GAAGC 991690 0.43021363 5.062987 30-34
TAAGG 938990 0.42978695 5.747636 10-14
AAGCT 815080 0.4165739 8.254062 30-34
TAAAG 638640 0.39782497 5.0659466 9
Produced by FastQC (version 0.10.1)