FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_18_SL248347.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_18_SL248347.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5573761
Filtered Sequences 0
Sequence length 251
%GC 54

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23458 0.420864834355115 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19794 0.3551282518213465 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19433 0.34865147608589603 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19432 0.3486335348788726 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 17061 0.30609493302637125 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15917 0.28557019219159196 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15481 0.2777478259293859 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15194 0.2725986995136677 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 14891 0.26716251378557493 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14746 0.2645610387671807 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14576 0.26151103357320127 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14411 0.2585507344143389 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14338 0.2572410263016301 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14238 0.25544690559928923 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14154 0.2539398442093229 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14019 0.2515177812611628 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13969 0.2506207209099924 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13918 0.24970571935179856 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13882 0.24905983589895583 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13501 0.24222423602303722 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13405 0.24050188014879 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13180 0.23646510856852312 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 13000 0.23323569130430957 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12575 0.22561067831936102 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12552 0.22519803055782261 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11659 0.20917653268591888 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11617 0.20842300199093575 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 10988 0.19713798277321182 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10905 0.19564886259026892 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 10823 0.19417768361434945 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 10802 0.19380091826685786 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 10719 0.19231179808391496 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 10382 0.18626561131702635 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10311 0.18499178561836432 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10106 0.1813138381785656 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 10054 0.18038089541334837 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 9900 0.17761794953174348 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9833 0.1764158886611751 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9796 0.175752064001309 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 9748 0.17489088606418537 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 9723 0.17444235588860016 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 9635 0.17286352967054022 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9554 0.17141029190164414 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 9481 0.17010058378893533 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 9457 0.1696699948203735 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9367 0.16805528618826676 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 9003 0.1615246868317461 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 8818 0.15820556353241555 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 8753 0.157039385075894 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 8509 0.15266173056218235 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 8289 0.14871466501703248 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 8141 0.14605936637756803 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 8060 0.14460612860867197 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7751 0.13906229563843875 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 7602 0.1363890557919509 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 7579 0.1359764080304125 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7488 0.13434375819128233 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 7482 0.13423611094914187 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 7446 0.13359022749629917 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7337 0.13163463593074765 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7322 0.13136551782539654 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 7147 0.12822580659630006 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 7127 0.1278669824558319 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6946 0.12461962398459497 No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG 6641 0.1191475558424554 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6562 0.11773020048760613 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6240 0.1119531318260686 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6069 0.10888518542506577 No Hit
AAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 5617 0.10077575985048516 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 11200350 7.587067 66.47625 35-39
TATTA 5553410 7.2026386 125.165436 240-247
TGGAT 8493990 7.1141 78.964294 35-39
TCACC 8675160 6.406474 74.456924 160-169
TCTCC 7719055 6.134981 87.58671 20-24
CTGTG 8110965 5.913196 87.47094 25-29
TCCCT 7266910 5.7756233 72.19504 15-19
AGTGG 8734315 5.666587 39.580807 100-109
CTGAG 8241680 5.582877 50.036022 15-19
TGTAT 5087285 5.500598 58.92709 240-247
TTACT 4644665 5.24358 110.76258 240-247
ATTAC 4710025 4.9407086 103.876595 240-247
TGGGT 7031095 4.90934 45.240837 100-109
CCTGA 6873440 4.861453 51.714493 15-19
CTCTG 6200290 4.7196674 73.423996 35-39
TTCAC 5123740 4.678371 74.65804 40-44
CTCCA 6227580 4.598974 41.372643 80-89
GACAC 6944175 4.5635753 46.438393 230-239
ACACG 6943475 4.5631146 48.859894 230-239
TGAGA 5666515 4.4097795 93.13831 15-19
ATTCA 4123910 4.325887 111.39524 40-44
TCTGG 5924210 4.3189707 134.51035 35-39
TACTG 4865025 4.254445 78.18423 240-247
AAGGG 7029790 4.2376747 38.826675 90-99
CCCTG 6774570 4.17076 85.03006 10-14
AGCCT 5871970 4.1531334 51.013737 30-34
CAGCC 7248315 4.146323 55.60658 30-34
TGGAG 6332795 4.1085453 38.354458 90-99
CCAGG 7494130 4.1057987 52.850483 80-89
GAAGG 6798035 4.097969 30.76922 150-159
ACCAT 4776470 4.052352 66.75516 160-169
GAGAC 6355800 4.0004163 83.8439 8
TCCAG 5614795 3.9712374 40.711163 80-89
TCAGT 4538025 3.9684854 95.78749 7
GGGTC 6958175 3.9294307 125.49978 8
CACCA 5695805 3.908312 56.97903 160-169
GTATT 3607725 3.900832 68.227425 240-247
ACTAC 4576430 3.8826382 34.702934 130-139
TACTA 3645895 3.82446 28.463505 130-139
CTGGG 6729165 3.8001032 132.98709 3
CACCT 5123395 3.7835495 44.03838 40-44
GACTC 5329115 3.7691824 52.005756 20-24
GATTC 4264845 3.7295907 58.926796 40-44
ACTGT 4238155 3.7062502 67.11633 240-247
AGACT 4548460 3.6958544 68.5743 15-19
TGAAG 4745070 3.692695 39.276447 150-159
GGACA 5706950 3.5920222 27.72999 220-229
GCTGG 6313705 3.565484 33.26642 90-99
CAAGA 4697730 3.5467536 43.472107 180-189
CCTGT 4627315 3.5223172 62.42141 25-29
ACTGG 5155505 3.4923162 43.623795 60-69
ACTCT 3777785 3.4494095 68.50951 20-24
GAGTG 5250210 3.406194 38.57239 90-99
TCCTG 4424700 3.3680866 64.40758 20-24
TATCT 2946030 3.325911 49.13817 200-209
CTCCT 4141900 3.2919157 94.678246 20-24
GGTCC 5529975 3.2606719 143.30548 9
GGATT 3870695 3.241882 56.252975 40-44
CTGCA 4566450 3.2297635 44.59852 25-29
AGACA 4274840 3.227475 37.07912 170-179
AGCTG 4725360 3.2009382 36.19357 60-69
CATCT 3491495 3.1880047 33.2635 170-179
GTCTC 4090860 3.1139672 32.52719 30-34
CCATC 4216435 3.1137733 34.26193 45-49
GTCCC 4982545 3.0675015 75.39423 10-14
CCTCA 4040675 2.9839776 81.49706 5
GGAGT 4586180 2.9753897 36.77642 90-99
GGCTG 5196475 2.9345608 34.3421 90-99
AAGAA 3322985 2.8822384 36.29187 180-189
AGGGA 4737320 2.8557355 39.4652 80-89
ATCTC 3123600 2.8520882 48.357956 170-179
GTGTA 3362700 2.8164132 35.456413 230-239
AGTAG 3614880 2.8131618 27.816431 50-59
TGTGC 3854125 2.8098013 57.59351 25-29
GAGCT 4133205 2.799815 29.195026 60-69
GTGAA 3575610 2.7826009 38.530197 150-159
ATCTG 3178120 2.7792535 37.045834 200-209
GGAAG 4538910 2.736131 29.582262 80-89
GACAA 3602930 2.7201874 24.519995 180-189
CAGGG 5178895 2.717465 35.859253 80-89
ACCTT 2944165 2.68825 71.39355 40-44
CTATG 3059455 2.6754813 27.765995 50-59
GAACA 3543490 2.6753106 34.84346 210-219
ATGAA 2788170 2.6027246 27.485357 210-219
GGGAA 4313610 2.600316 33.17845 80-89
CACGG 4667370 2.5571058 44.13661 230-239
CGCCA 4467885 2.555807 40.016975 70-79
TGCAA 3129670 2.5430155 35.62841 200-209
GAGTC 3729000 2.5260084 27.056295 150-159
CCCTC 3903010 2.5088968 27.266953 20-24
AGGGC 4778885 2.5075722 32.66195 150-159
AGAAC 3313340 2.5015492 29.985687 190-199
CAGTG 3678205 2.4915998 74.15872 8
AGGCT 3643505 2.4680943 20.205904 25-29
ATCAG 3018660 2.452814 25.898638 45-49
TATAT 1884000 2.4435027 24.050285 240-247
CCAAG 3715700 2.441885 38.18347 180-189
ACGGC 4445215 2.435394 48.41418 230-239
CTACA 2840580 2.4099448 19.397545 190-199
GTGGA 3683815 2.3899593 26.088943 100-109
TCTGT 2532225 2.3832393 24.022688 210-219
CACTG 3362525 2.37825 19.421335 60-69
CTCTC 2984495 2.3720284 98.357315 20-24
CCGCC 4751215 2.365769 34.339355 70-79
TGAAC 2904975 2.3604393 37.89503 210-219
GCAGC 4301595 2.3567092 34.47641 30-34
GTATC 2686095 2.3489797 23.006184 200-209
ACAGC 3557380 2.3378398 26.992899 210-219
CTGTC 3070285 2.3371043 47.743477 30-34
TATGC 2655135 2.3219054 21.150986 50-59
CTACT 2538980 2.318285 22.552673 50-59
GGGCT 4077805 2.302824 42.437763 90-99
CTTCA 2514615 2.296038 50.516006 45-49
GTGGT 3287310 2.295307 30.936666 120-129
CAAAT 2339795 2.280533 34.796688 200-209
CAGGC 4144920 2.2708719 33.341625 70-79
GGCCG 4971035 2.2704668 24.91653 230-239
TCCAA 2672155 2.2670534 42.157894 180-189
TGGTG 3246775 2.2670043 33.336086 35-39
GCCTC 3658330 2.252249 71.93054 4
GCCTG 3779460 2.2285051 31.080126 210-219
AATGA 2364155 2.2069113 25.92495 210-219
GCTCC 3576245 2.2017136 34.226517 80-89
AAATG 2345665 2.189651 33.029087 200-209
TATCA 2086785 2.1889896 16.70269 170-179
ACAAC 2766050 2.1804829 19.703749 180-189
AAAAA 2088970 2.1734107 17.365282 90-99
GGGGG 5156900 2.1605175 94.94066 5
CAGTA 2642750 2.1473684 20.978304 45-49
CCTTC 2688800 2.1370149 55.56206 45-49
TACAT 2034955 2.1346211 18.14441 190-199
GTGGG 3907655 2.113493 30.079819 100-109
GCTAT 2408840 2.1065214 33.765285 50-59
CCTCT 2642170 2.0999544 49.12425 35-39
TGCAG 3098360 2.0988152 43.324726 30-34
GTAGT 2485130 2.0814087 24.942715 120-129
GCTGT 2845725 2.0746398 19.229889 230-239
GCAGA 3291955 2.0719955 24.275167 140-149
AACAG 2703015 2.040758 33.453312 210-219
TCTAT 1801155 2.0334082 19.708838 110-119
CTCAC 2752115 2.0323954 54.638523 20-24
TGCAC 2853345 2.0181167 25.705301 25-29
CAGAG 3151290 1.9834595 32.28396 170-179
AGAGT 2544560 1.9802203 29.39309 150-159
GGCTC 3344660 1.9721316 19.45884 40-44
AACTA 2019915 1.9687549 35.808228 130-139
AGCAC 2985080 1.9617358 20.24345 130-139
TGTGA 2341755 1.9613258 18.351713 210-219
ATGGA 2518040 1.9595821 12.123014 100-109
TCCGC 3181500 1.9586887 42.27462 70-79
TGGTA 2325490 1.9477029 30.650082 120-129
TTCAG 2221455 1.9426538 47.52438 45-49
TGAGC 2864140 1.9401559 17.383535 200-209
CCGTG 3282900 1.9357156 19.759792 230-239
CAGAC 2943905 1.9346765 26.535429 140-149
TCTCT 1966040 1.932001 32.412586 35-39
CTGTA 2208215 1.9310753 24.390682 190-199
GGTCT 2645395 1.928592 35.761288 15-19
ATATC 1825725 1.9151436 13.25559 170-179
CATAT 1820785 1.9099617 13.725616 160-169
GGTGG 3513785 1.9004648 18.020296 40-44
GCCAG 3448095 1.8891032 35.594715 70-79
CCAGA 2872975 1.8880627 33.91497 170-179
TGGGG 3463215 1.8731133 123.42061 4
GCACA 2849395 1.8725663 18.318834 130-139
GGATC 2760640 1.8700454 17.892736 70-79
ACTAT 1780030 1.8672106 17.33494 130-139
TCCAT 2028960 1.8525972 26.625437 45-49
CCTGC 3007710 1.8516951 49.25051 20-24
AGAGA 2556480 1.8485695 27.78722 170-179
ACCCT 2498025 1.8447536 42.961124 10-14
AGGAC 2928885 1.8434751 20.439465 220-229
GATGG 2837995 1.8412143 20.95892 100-109
GTCAC 2597060 1.8368512 37.520905 160-169
ACCAG 2790560 1.8339012 13.660024 190-199
CTATT 1622810 1.832066 19.393751 110-119
TCAGC 2567815 1.8161669 11.439129 45-49
CACAG 2755185 1.8106534 15.79931 140-149
TTCCA 1980955 1.808765 23.10871 180-189
GCTGA 2669475 1.8082905 26.44831 200-209
TGGGA 2786590 1.8078641 16.556387 100-109
CAGTT 2066670 1.807295 23.764856 190-199
GCCGA 3278130 1.7959846 19.348354 220-229
TGTCT 1904890 1.792814 48.412895 30-34
GAGGA 2960465 1.7846178 26.662506 220-229
ATACT 1697060 1.780177 26.513206 130-139
CAAGG 2803730 1.7647007 17.101248 80-89
TAGTG 2090485 1.7508757 25.80649 120-129
TAGTA 1738445 1.7465352 16.866571 120-129
TTCTG 1855460 1.7462924 42.94705 30-34
ACCTG 2462190 1.7414601 30.596281 25-29
GCAAA 2296915 1.734155 32.013138 200-209
CCTGG 2935470 1.7308582 33.716526 2
AGTCA 2102215 1.7081563 28.175451 160-169
CTGAA 2099070 1.7056007 22.488941 200-209
TACAA 1744515 1.7003301 16.928406 130-139
CTCAG 2403050 1.6996315 77.67346 6
TGACC 2400960 1.6981535 14.035235 210-219
CATGA 2087890 1.6965164 22.93915 60-69
ATGCA 2076250 1.6870583 16.373552 60-69
GTGCA 2487210 1.684825 50.25197 25-29
AGTTC 1924655 1.6831034 21.028492 190-199
AGCTA 2060755 1.674468 25.715183 50-59
AACAC 2109275 1.6627458 22.601206 190-199
GGATG 2555045 1.6576439 22.35125 100-109
GGAGC 3141040 1.6481636 13.142544 200-209
GCCGT 2791590 1.6460215 28.91588 230-239
TCTGC 2138420 1.6277678 33.771015 200-209
TCTCA 1780925 1.6261222 26.222605 100-109
ATTGG 1925345 1.6125635 19.781319 100-109
AGGGG 3196710 1.606499 32.665497 90-99
CAGCT 2257785 1.5968885 12.970767 50-59
GGGCC 3494210 1.5959429 28.022854 2
CGATT 1819580 1.5912157 35.086597 160-169
TCTGA 1816620 1.5886272 21.252132 220-229
GGGGC 3618050 1.5826807 28.642647 90-99
ACAAT 1596250 1.5558205 23.057104 180-189
CTCCC 2399060 1.5421416 13.5831375 190-199
GCACT 2168570 1.5337884 24.701946 25-29
CGTCC 2475030 1.523751 18.593086 180-189
CCATG 2152640 1.5225213 15.626336 160-169
GTTCT 1614460 1.5194718 22.293858 190-199
GGCTT 2077640 1.5146773 21.400915 25-29
TTCTC 1536030 1.509436 21.275543 190-199
GTCCG 2552255 1.504901 34.584003 70-79
CGGCC 3153740 1.5039861 31.462578 230-239
AGTTA 1492050 1.4989936 15.324578 50-59
AGTGA 1921465 1.4953171 84.956955 9
GAGCA 2357640 1.4839267 7.5857735 200-209
CTGGT 2027840 1.478371 30.821396 35-39
TTAGT 1367070 1.4781367 25.358267 110-119
CTTTA 1296895 1.4641255 41.941883 45-49
CATCA 1722240 1.4611465 30.079464 45-49
ACTCC 1970240 1.4549924 17.252638 140-149
TACGC 2045530 1.4467646 24.542696 140-149
GTTAC 1645490 1.4389747 15.253543 50-59
GGGGT 2589965 1.4008076 117.830894 7
GTGAC 2063235 1.397626 14.669544 210-219
CGTGT 1913415 1.394951 23.398853 230-239
GGCAG 2644470 1.3876039 13.484029 150-159
AAGAG 1918875 1.3875226 17.960138 150-159
CTCAA 1625915 1.3794245 18.275337 150-159
GTCCA 1949895 1.3791237 28.358435 180-189
GGCCT 2335785 1.3772626 68.76986 3
CTACG 1940240 1.3722948 16.112156 130-139
TAGCA 1687670 1.3713174 11.61278 130-139
GAGAG 2250070 1.3563799 25.528008 220-229
AACCC 1973350 1.3540612 19.638187 140-149
CAACC 1971200 1.3525858 15.051302 140-149
AGCAG 2140830 1.3474638 12.433792 50-59
CGACA 2047300 1.3454453 16.12895 70-79
GAACT 1646470 1.3378403 14.337054 190-199
AAGCT 1641445 1.3337573 15.994365 200-209
CAACT 1570940 1.3327836 16.943787 130-139
CAATT 1267485 1.3295625 24.359026 180-189
ATACA 1356495 1.322138 21.461454 35-39
ACAGT 1624290 1.3198178 12.113379 120-129
TGGAA 1692295 1.316973 19.171494 120-129
TGTGT 1452825 1.3095709 15.104955 240-247
CACGT 1848410 1.3073453 16.405836 180-189
TTATT 936400 1.3070757 20.557404 240-247
ATCTA 1245815 1.3068312 20.075947 110-119
GTGCG 2313300 1.3063699 16.921955 240-247
AGAGC 2073615 1.3051579 24.566437 220-229
TTACA 1243625 1.3045341 9.566099 120-129
ACAGA 1727350 1.3041372 20.010166 140-149
TCACT 1422200 1.2985785 13.267123 190-199
CCATA 1526625 1.2951869 14.2408695 160-169
CCTTT 1316685 1.2938884 48.209984 45-49
GTGGC 2290910 1.2937257 14.177154 40-44
TCCGT 1699500 1.2936612 13.982079 150-159
AATTC 1228165 1.288317 24.359804 180-189
TGTCC 1686420 1.2837049 53.54129 15-19
ACATG 1579415 1.2833546 14.3228035 190-199
TCAAG 1578880 1.28292 22.481909 150-159
ATCAT 1215475 1.2750055 15.826832 110-119
CCTAC 1720595 1.2706333 17.296991 190-199
TGCGA 1862065 1.2613546 13.912722 240-247
ATATT 971950 1.2605957 25.79406 240-247
AACCA 1587540 1.2514611 16.955286 190-199
AGACC 1902565 1.2503283 96.794975 9
ATTAT 962695 1.2485923 9.641171 50-59
ACATA 1277390 1.2450364 20.5391 130-139
CTTCT 1261885 1.2400374 23.305187 30-34
CCAGT 1746270 1.2351036 18.203575 190-199
ACACA 1562270 1.2315406 7.633318 130-139
CGGCT 2085615 1.2297534 21.198673 230-239
ACGTC 1727395 1.2217537 18.183811 180-189
GATCT 1386975 1.2129043 9.290795 220-229
ATCCG 1706340 1.2068617 23.23057 70-79
GATTG 1437900 1.2043064 22.878202 100-109
AGTCG 1773525 1.201378 15.496555 150-159
TCGGA 1772805 1.2008902 90.27277 5
TACCA 1412155 1.1980708 11.064867 45-49
TGGAC 1761180 1.1930153 15.790631 80-89
TGAGT 1410805 1.181613 20.665863 90-99
AGATC 1451190 1.1791655 12.35008 210-219
CGCTG 1994595 1.1760848 12.543827 190-199
AAGGC 1861245 1.1714896 28.373274 25-29
ATTAG 1158665 1.1640571 24.961742 110-119
GTCGA 1714320 1.1612728 12.041583 150-159
GGGTG 2145920 1.1606417 14.511619 60-69
ATTCC 1269145 1.1588275 22.631516 180-189
AGGCC 2102655 1.151979 14.065448 2
GATCC 1627360 1.1510009 23.05544 70-79
CTTCG 1508340 1.1481502 102.30505 3
ACGCA 1746180 1.1475551 20.878407 140-149
GAAGT 1461520 1.13738 13.252701 140-149
AGCTC 1582570 1.1193218 12.090271 210-219
GACCC 1955645 1.1187065 42.44657 10-14
GCAAG 1771355 1.1149119 16.789354 25-29
TCGAG 1644450 1.1139431 11.730058 150-159
AGGTC 1643580 1.1133538 18.007992 10-14
GGACT 1641930 1.1122361 12.568002 90-99
GAACC 1684390 1.106948 10.074018 180-189
ACAAG 1462105 1.1038792 17.169756 80-89
TTGGG 1572135 1.097716 14.770663 100-109
CGCAG 1998275 1.0947922 16.537958 140-149
GCCCC 2198170 1.0945331 12.220274 80-89
CCGAT 1547415 1.0944573 23.559517 160-169
GTATA 1086120 1.0911745 9.948226 240-247
CACAT 1285320 1.0904641 13.742411 130-139
GCTTC 1427130 1.0863329 26.67445 2
TTCGG 1488515 1.0851831 97.91714 4
CTTGA 1234885 1.0799022 22.023842 90-99
TTGAG 1285715 1.0768446 20.390423 90-99
GCACC 1878200 1.0744047 10.924529 130-139
CCCCT 1669515 1.0731822 9.6944 70-79
TACAG 1306345 1.0614715 9.119529 120-129
CTCCG 1723145 1.0608532 10.04069 140-149
ATGGG 1634780 1.060601 19.863674 100-109
CAGCA 1604260 1.0542881 12.062361 45-49
TAGAC 1296810 1.0537238 16.764343 170-179
GAAGC 1673755 1.0534812 12.484571 200-209
ATCAA 1079215 1.0518808 12.201752 110-119
ACACC 1528040 1.048501 12.809505 40-44
ATGGT 1241845 1.0401013 9.376286 35-39
CTATA 986125 1.0344224 13.201208 50-59
GAGCC 1886620 1.0336201 18.002123 220-229
CGTGA 1524685 1.0328149 20.863762 150-159
ATGAG 1326630 1.0324062 19.733753 60-69
GTAGA 1326065 1.0319666 16.38082 170-179
GGAGA 1707935 1.0295718 80.167274 7
TGGCT 1410015 1.0279536 30.56114 40-44
GACAG 1618985 1.0190084 15.476121 70-79
GCTCT 1327830 1.0107458 16.778465 210-219
TTTCA 894700 1.0100687 7.22904 100-109
GGGAG 2006970 1.008598 14.83854 5
GTAGC 1488820 1.0085201 11.225365 50-59
ACCGC 1762145 1.0080167 10.926855 220-229
AGCCG 1839345 1.0077195 18.973604 220-229
ACTCA 1186180 1.0063537 12.2215185 190-199
AAACT 1029615 1.0035372 16.955849 130-139
CGAGG 1912520 1.0035357 14.573465 220-229
TGAGG 1538645 0.9982312 11.7185545 220-229
TTATA 765615 0.9929842 13.310656 110-119
TAGTT 915805 0.9902089 14.265484 50-59
ATGAC 1218280 0.98991424 10.854795 160-169
ATGAT 974720 0.97925603 9.015613 120-129
AACTC 1147770 0.97376686 16.072405 190-199
AAGGT 1243010 0.9673317 20.437952 15-19
GCCAA 1470395 0.9663147 14.736568 180-189
CCGTC 1567635 0.96511376 10.307781 140-149
CCGAG 1750825 0.95922214 17.889877 220-229
GCAGT 1415480 0.95883983 6.220511 50-59
GATAC 1178500 0.957591 23.457226 35-39
GGTAT 1139010 0.9539724 9.103235 110-119
CGAGT 1408030 0.9537933 14.73635 160-169
TTTAG 881685 0.95331687 29.452684 45-49
GCCAT 1344845 0.9511834 7.718116 60-69
ACCAA 1203750 0.94891864 16.336834 130-139
TTTAT 675395 0.9427514 11.100533 240-247
CGTAT 1070975 0.9365635 7.3940845 240-247
CATAC 1102080 0.9350035 13.996908 130-139
CAGAA 1236905 0.9338548 17.905806 140-149
ACAGG 1483395 0.9336663 14.986259 70-79
GGGAC 1777160 0.9325097 9.642081 90-99
CGGAC 1699985 0.9313684 10.547963 220-229
CCAAC 1349715 0.9261392 13.52147 130-139
AACTG 1139330 0.92576337 11.603644 60-69
TATGG 1103610 0.92432326 12.099941 35-39
AGCCC 1612395 0.92235386 11.169639 80-89
TAACA 943370 0.91947645 8.730916 120-129
TACAC 1078835 0.9152825 21.51563 35-39
TCATA 866800 0.90925324 8.848905 110-119
GAGAT 1165080 0.90668535 11.930592 210-219
AATCA 927955 0.9044519 13.529871 110-119
CCCAG 1578825 0.9031504 14.355665 80-89
TGGTT 997060 0.89874595 9.035732 50-59
ACGCT 1261075 0.8919344 15.318497 190-199
GTTAT 820990 0.8876907 10.829316 110-119
CCCCA 1479005 0.8833755 16.563236 80-89
TATTT 632405 0.8827438 10.816815 240-247
AAGTT 877630 0.8817142 17.129782 140-149
TCCTT 896300 0.88078177 10.49185 45-49
CATTA 831435 0.8721562 12.261179 110-119
CGGAG 1655830 0.8688454 69.880394 6
GACTG 1282315 0.8686346 12.19674 90-99
GTCTG 1190760 0.8681085 16.174747 3
AGTCT 989095 0.8649598 16.38365 8
AGAAG 1193420 0.8629522 15.539184 140-149
GATGA 1106845 0.86136585 6.568147 60-69
TTACC 940355 0.8586168 10.234915 45-49
GATCA 1043120 0.8475879 9.340622 110-119
GCCTA 1196115 0.84598947 16.53014 190-199
GTTTC 897740 0.84492064 5.0551586 100-109
TCTTC 857895 0.84304184 46.292774 2
GGTAG 1295620 0.8405631 11.829945 120-129
GGTTC 1152210 0.8400042 11.483672 160-169
CATGG 1239265 0.8394726 8.608029 200-209
ATAGT 835475 0.83936304 15.161094 120-129
ATCCA 982295 0.83337796 9.671419 180-189
CTATC 907940 0.82901937 8.351308 110-119
ATATG 816955 0.8207568 5.6544185 50-59
GCTTG 1124740 0.81997746 16.934853 90-99
TCATC 897035 0.81906223 10.600747 110-119
GGTGC 1448860 0.8182023 11.607307 60-69
TGGCA 1206345 0.81717277 5.942942 100-109
ATATA 677565 0.8165361 10.6650305 130-139
GTCTA 932230 0.8152315 9.764416 30-34
ACAAA 900055 0.81511736 12.695661 130-139
ACATC 953490 0.8089398 5.614004 110-119
TTATG 745925 0.80652714 11.436797 50-59
TGATG 945640 0.79201627 12.102979 120-129
CCTCC 1221000 0.78487194 10.329505 140-149
TATAC 737255 0.77336365 8.95804 130-139
CTGAC 1092880 0.7729733 9.717233 220-229
GTTCA 872305 0.76282734 13.819515 160-169
CCAGC 1321085 0.7557129 6.2717376 80-89
GGGAT 1149315 0.7456445 13.297013 100-109
GGTTA 882395 0.73904574 8.00996 50-59
TCAAC 868555 0.73688114 7.5322776 110-119
CGCCG 1544685 0.73664445 10.77674 220-229
ATAAA 654730 0.7331271 8.005536 130-139
ATCTT 646180 0.7295028 12.485589 1
TTGGA 869150 0.7279525 6.39425 60-69
TTTAC 643710 0.7267143 13.738018 45-49
GATTA 721985 0.72534496 5.654047 50-59
GCGGA 1379445 0.72382104 8.762507 220-229
ACGCC 1263615 0.7228378 12.991975 180-189
CCCCC 1384825 0.71996623 13.130004 80-89
AACGC 1091255 0.71715134 14.365112 180-189
ATGCC 1011780 0.71561277 8.210338 50-59
CATCC 967445 0.7144435 6.598022 110-119
GACCA 1083480 0.7120418 6.134191 170-179
TATTG 658360 0.71184796 6.455679 240-247
GACCG 1294155 0.709027 9.030437 210-219
TGCCA 998430 0.7061706 7.40741 60-69
AAATA 627915 0.7031013 12.601818 130-139
GGGCA 1339070 0.7026356 13.470098 150-159
AATAC 720185 0.7019443 11.332467 130-139
CACAA 886710 0.69899535 8.2118845 130-139
TTTCT 574595 0.6981403 9.601326 2
CAACG 1061025 0.6972847 14.63228 180-189
TCCGG 1180320 0.6959589 11.309229 70-79
GGATA 891600 0.69385844 15.337937 35-39
CAAAC 869775 0.6856454 13.711136 130-139
GAAAT 731520 0.6828655 7.0131845 110-119
ATTAA 560900 0.6759428 6.5614586 110-119
CAGTC 951010 0.6726313 5.464562 210-219
TATAG 669440 0.6725554 6.0623536 120-129
CCCGT 1087070 0.669254 10.1189995 140-149
AGGCA 1061410 0.6680641 6.68591 80-89
CACGC 1165970 0.666981 12.474806 190-199
AATAA 595060 0.6663123 7.4683022 130-139
GCCGC 1394535 0.6650395 11.904455 220-229
GCGAC 1189005 0.6514185 7.164762 70-79
GACGA 1030860 0.64883554 9.828223 220-229
AGATG 829480 0.6455156 5.5263376 120-129
ACTTC 699520 0.6387158 28.852772 4
CCGTA 897575 0.63483775 6.269157 230-239
TACTT 557330 0.62919587 11.324529 1
CACCG 1091605 0.62444127 9.024609 45-49
TCAAT 591235 0.62019193 8.153912 110-119
TCCAC 833940 0.61585206 10.300576 180-189
AAGCA 814305 0.61479473 5.868636 120-129
AGTAA 653625 0.6101514 5.2292137 50-59
CCGGC 1272665 0.6069209 9.193932 70-79
TAGCT 692235 0.6053568 12.4013195 50-59
CACGA 918825 0.60383373 8.775019 180-189
GGCCC 1260105 0.60093117 10.376124 70-79
GAAGA 828820 0.59931284 6.5180354 15-19
GGTGA 923340 0.599038 6.4872375 120-129
CCCTA 810965 0.5988854 11.636554 110-119
TGATT 552970 0.5978956 6.0589147 50-59
AATGG 763535 0.594196 6.7054725 120-129
ACGAC 899665 0.59124213 9.200308 220-229
ACCCG 1018555 0.58265376 8.650215 140-149
CTTTG 618825 0.5824159 7.31973 45-49
TAATA 479700 0.5780883 8.156714 130-139
ATTTC 510675 0.57652485 6.4428473 240-247
GGGGA 1145815 0.5758265 9.597418 4
ACCTA 671830 0.56997985 6.9033704 130-139
GTCCT 747410 0.5689294 6.2950196 45-49
CGGTT 770740 0.56189823 11.371133 160-169
GTTCC 735690 0.5600081 6.5782866 140-149
TCAGG 824615 0.55859053 5.47512 150-159
TTAGC 636755 0.55683976 9.597889 45-49
TTCAA 529415 0.55534416 5.205612 200-209
AAGAT 585915 0.54694486 5.9463744 15-19
CTGCG 918200 0.54140365 11.852914 25-29
CCACG 944410 0.54023993 7.756501 180-189
CGGCA 978635 0.5361634 10.49751 70-79
GGAGG 1060260 0.53283113 5.3599997 6
GCTAC 753060 0.5326251 7.8782315 50-59
AAATC 541465 0.5277509 7.6055737 110-119
CTGTT 559210 0.52630836 5.602018 240-247
TATGT 478655 0.51754296 6.42479 140-149
ACATT 488170 0.5120791 6.073461 110-119
GGGTT 729735 0.5095248 5.9882164 100-109
ACCCC 849165 0.50718665 8.735479 140-149
TGACG 735180 0.49800766 8.774278 220-229
GTAAC 604865 0.49148345 6.84336 120-129
CGCGG 1061535 0.4848447 6.3477225 220-229
TATCC 528125 0.48221895 5.486202 110-119
TGGCC 811665 0.47858676 15.142105 2
TAAAT 391120 0.47134018 7.9947915 130-139
AACAT 482065 0.46985534 5.5370812 110-119
CCGGT 792730 0.467422 8.826119 160-169
ATGCT 523050 0.4574052 7.0119157 50-59
CAATC 536855 0.45546716 6.166782 110-119
GGTTT 505205 0.45538986 5.380338 100-109
CATAA 464895 0.4531202 5.7513328 110-119
ATTCT 398360 0.44972724 7.9242334 2
TGCTA 514040 0.44952595 6.1930456 50-59
CCGCG 937435 0.44705313 6.6114 220-229
TGCGC 748070 0.44108889 8.763117 25-29
TTGGT 479670 0.43237272 5.1262407 120-129
GGTCA 636755 0.43133506 5.49677 160-169
GCCTT 556185 0.4233687 8.356405 1
GCATG 621380 0.42092004 8.282719 60-69
CACTT 459880 0.41990596 6.820688 3
GCGCT 710110 0.4187063 7.937141 25-29
TACCT 453865 0.4144138 5.191043 2
CAATG 498935 0.40540993 5.051364 120-129
ATGGC 597410 0.40468293 7.131604 1
ACGAG 642505 0.4044003 7.6528454 180-189
CTAAC 473940 0.4020902 5.3689427 110-119
AGCTT 452005 0.39527667 6.8998704 1
CCGGG 864915 0.39504063 5.288959 80-89
CATGC 557580 0.39436576 7.0119743 60-69
TTTGA 360745 0.39005345 6.1165905 45-49
CCTAA 459010 0.38942358 7.456631 110-119
GGTAA 491030 0.382128 7.1930227 120-129
GAGGC 718290 0.37690046 5.7324123 35-39
TTCTT 296915 0.36075556 18.626482 1
TGGGC 619820 0.3500256 9.438461 1
CTCGG 592815 0.34954494 5.159262 6
TGCTG 475635 0.34675568 13.537413 1
CGAGC 627100 0.34356847 6.5387206 180-189
GCTTT 329800 0.3103959 5.13283 45-49
CTCTT 313905 0.30847016 9.101832 1
GCGAG 579555 0.3041035 5.3178134 240-247
TCACA 358270 0.30395585 5.1400137 7
CGCTT 398295 0.30318263 6.037011 1
AACTT 287060 0.30111936 10.3169565 3
GACTT 338785 0.2962662 6.615574 1
ATTGT 268190 0.2899789 5.310233 240-247
TTCCT 285390 0.2804489 6.387927 4
GAGGT 425735 0.27620536 6.7849636 7
TGTAA 268175 0.269423 8.172311 25-29
TAACT 256360 0.26891577 7.0040326 2
TCGGT 366650 0.26730153 6.047636 7
CCCTT 333615 0.26515183 5.6994762 3
GTCTT 279700 0.2632436 12.200419 1
TTGGC 358755 0.2615458 6.9174004 1
CGGGG 585450 0.25609934 9.583137 3
AATCT 243770 0.25570914 7.3564367 2
CCTCG 401395 0.24711862 5.633259 5
TCCTA 253770 0.2317116 5.0683312 9
TGCTT 233250 0.2195265 10.510021 1
TCGCA 264000 0.18672219 6.8333716 7
TAGGC 234520 0.15886283 7.0118647 1
TTGCT 137430 0.12934418 5.4484363 4
Produced by FastQC (version 0.10.1)