FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_13_SL248342.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_13_SL248342.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10666444
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 45439 0.4259995177399328 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 36156 0.3389695759898988 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 35790 0.3355382543610598 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 34535 0.32377238374851075 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 34190 0.32053794122952317 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33526 0.3143128112799355 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33299 0.3121846418543987 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 32367 0.30344695945527866 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30724 0.28804351290833197 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30180 0.2829434064436095 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 28207 0.2644461453132834 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27655 0.25927103728290324 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27617 0.2589147798460293 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27230 0.2552865791073389 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25822 0.24208630355158664 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25734 0.2412612863293521 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25671 0.24067064899979787 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25400 0.23812997096314387 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25109 0.23540178901234562 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 24621 0.23082669350722698 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24322 0.22802350999077103 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24020 0.22519220088719352 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23955 0.22458281316622483 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23872 0.2238046719225264 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23477 0.22010146961817825 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23237 0.21785142264844778 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22730 0.2130981984248921 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21758 0.20398550819748362 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21664 0.20310423980100584 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21371 0.2003573074587932 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21207 0.1988197753628107 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20738 0.19442280857612904 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20487 0.19206963445361921 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20394 0.19119774125284864 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20030 0.18778517001542405 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19563 0.18340695362015683 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19476 0.1825913115936295 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 18811 0.1763568064483346 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18771 0.1759817986200462 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18267 0.17125669998361218 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 18086 0.16955978956060708 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17891 0.16773162639770106 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 17748 0.16639097341156997 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17740 0.1663159718459123 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17674 0.1656972089292364 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17446 0.16355966430799243 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 17058 0.1599220883735948 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 16625 0.15586262863237269 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16543 0.15509386258438146 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16534 0.15500948582301657 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16030 0.15028438718658252 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15508 0.1453905350274187 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15220 0.1426904786637421 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 15159 0.1421185917256023 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14974 0.14038418051976836 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14572 0.13661535184546977 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 14308 0.13414030017876624 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14209 0.1332121558037524 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13999 0.13124336470523823 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 13963 0.13090585765977866 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13730 0.12872143705999864 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 12950 0.12140878440837452 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12746 0.1194962444841036 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12641 0.11851184893484651 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12591 0.118043089149486 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 11841 0.11101169236907821 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10884 0.10203963007727787 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10732 0.1006146003297819 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10707 0.10038022043710162 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 21745730 7.7038093 66.59071 35-39
TATTA 10703615 7.330785 128.27454 240-247
TGGAT 16618530 7.301114 79.420906 35-39
TCACC 16784340 6.4372997 74.606575 160-169
TCTCC 14921735 6.1121416 86.382645 20-24
CTGTG 15669135 5.9285855 86.19446 25-29
TCCCT 14157460 5.799084 72.49788 15-19
AGTGG 16763880 5.7078424 40.180996 100-109
CTGAG 16019870 5.675322 50.003498 15-19
TGTAT 9836165 5.5759816 61.121124 240-247
TTACT 9025780 5.3237085 111.98832 240-247
ATTAC 9053490 5.000011 104.94339 240-247
TGGGT 13509465 4.9125867 45.033375 100-109
CCTGA 13278905 4.8947186 51.501705 15-19
CTCTG 12094680 4.761398 73.76708 35-39
TTCAC 9868620 4.693761 74.576324 40-44
GACAC 13524010 4.667629 47.30497 230-239
ACACG 13376985 4.616886 49.69682 230-239
CTCCA 11978885 4.594263 40.84489 80-89
TGAGA 10883275 4.476942 94.07956 15-19
ATTCA 7932220 4.3807626 111.34479 40-44
TCTGG 11423230 4.3221025 135.26593 35-39
TACTG 9435125 4.3129873 78.87411 240-247
AAGGG 13509195 4.3067765 39.858795 90-99
AGCCT 11386315 4.197093 50.744907 30-34
GAAGG 13084235 4.171298 31.704098 90-99
CCCTG 13134030 4.169395 84.48516 10-14
CAGCC 14003250 4.162261 54.83923 30-34
TGGAG 12211375 4.1577854 38.28635 90-99
CCAGG 14477120 4.135696 52.64434 80-89
ACCAT 9229975 4.11046 67.14127 160-169
GAGAC 12196515 4.04569 86.49066 8
TCAGT 8766415 4.007306 100.016335 7
GTATT 7048465 3.9956744 70.04857 240-247
TCCAG 10833655 3.9933789 40.404243 80-89
CACCA 11059285 3.9714756 57.27847 160-169
ACTAC 8838675 3.9361994 34.36154 130-139
GGGTC 13269240 3.8909273 124.213875 8
TACTA 7043980 3.89021 28.250671 130-139
CACCT 9960900 3.820305 43.743706 40-44
CTGGG 13014650 3.816274 130.97232 3
ACTGT 8283495 3.7865539 68.03697 240-247
GACTC 10175480 3.7507694 51.916985 20-24
TGAAG 9114050 3.749154 39.27692 150-159
GATTC 8183270 3.7407386 58.780006 40-44
GGACA 11199495 3.7149706 28.808311 220-229
AGACT 8617945 3.6885836 68.939575 15-19
CAAGA 9010740 3.6111193 43.61487 180-189
ACTGG 10093740 3.5758858 45.155342 60-69
GCTGG 12034160 3.528766 33.23096 2
CCTGT 8871620 3.4925528 61.328205 25-29
ACTCT 7319095 3.4811435 68.29414 20-24
GAGTG 9947585 3.3869991 39.293568 90-99
TCCTG 8573290 3.3751075 64.41053 20-24
TATCT 5629575 3.320513 48.50012 200-209
CTCCT 8029700 3.289072 93.84167 20-24
AGACA 8162890 3.2713368 37.295296 170-179
GGATT 7445385 3.2710238 56.306644 40-44
CTGCA 8807560 3.2465427 46.128937 25-29
GGTCC 10465465 3.193005 139.99245 9
CATCT 6670360 3.172589 32.954845 170-179
AGCTG 8934040 3.1650412 35.39763 60-69
GTCTC 7993710 3.14694 33.86413 30-34
CCATC 8077445 3.0979433 35.500053 45-49
GTCCC 9683445 3.074008 74.254555 10-14
CCTCA 7947070 3.0479403 84.525375 5
GGAGT 8860090 3.0167243 36.536957 90-99
GGCTG 10137275 2.972544 34.322517 90-99
AAGAA 6276430 2.9206827 36.86573 180-189
AGTAG 7036300 2.8944511 28.887875 50-59
AGGGA 8983720 2.86404 40.08448 80-89
GTGAA 6800125 2.7972987 40.440773 10-14
TGTGC 7391150 2.796521 56.64778 25-29
ATCTG 6093040 2.7852523 36.361485 200-209
GAGCT 7858090 2.783867 28.460636 60-69
GACAA 6925060 2.7752671 24.801697 180-189
CAGGG 10100490 2.7731597 36.10515 80-89
ATCTC 5766825 2.742845 47.88474 170-179
GGAAG 8579165 2.7350667 29.989164 80-89
GTGTA 6220495 2.7328856 36.30522 230-239
ACCTT 5645405 2.6850948 72.10942 40-44
GAACA 6682685 2.678134 34.791424 210-219
ATGAA 5373470 2.6705527 27.634552 210-219
CTATG 5730295 2.619434 26.239359 50-59
GGGAA 8179055 2.6075103 33.756695 80-89
GAGTC 7353915 2.6052542 27.502886 150-159
CACGG 9107695 2.6018062 44.732777 230-239
TGCAA 6008075 2.571528 35.286526 200-209
CGCCA 8647975 2.5704837 39.472973 70-79
AGGGC 9276910 2.5470402 33.109222 150-159
CAGTG 7131970 2.5266263 77.46653 8
AGAAC 6297275 2.523678 30.816893 190-199
CTACA 5616470 2.5012283 20.45765 190-199
CCCTC 7567630 2.4995909 27.282238 20-24
AGGCT 7031355 2.4909816 21.037083 25-29
ACGGC 8665415 2.4754593 49.088062 230-239
ATCAG 5746495 2.4595685 27.159176 45-49
TCTGT 5033940 2.457611 24.354767 210-219
CCAAG 7102070 2.4511836 38.12816 180-189
GTGGA 7195905 2.450095 26.863132 100-109
CACTG 6634545 2.4455507 19.780508 30-34
CTACT 5066920 2.4099526 22.742031 50-59
CTTCA 4987250 2.3720598 50.778625 45-49
CTGTC 6015980 2.3683531 48.499813 30-34
TATAT 3452035 2.3642597 23.23602 240-247
GGGCT 8041235 2.357924 42.612656 90-99
TGAAC 5505780 2.3565395 37.507725 210-219
ACAGC 6818920 2.3534584 26.484734 210-219
CCGCC 9168550 2.3469868 33.761726 70-79
CTCTC 5714845 2.3408768 96.964035 20-24
GCAGC 8178125 2.3362546 33.911423 30-34
CAAAT 4435755 2.2937598 35.079117 200-209
ACAAC 5439460 2.2681468 21.050861 180-189
TATGC 4957550 2.2661967 20.363747 50-59
CAGGC 7927610 2.2646897 33.53256 70-79
GGCCG 9571680 2.263238 25.102652 230-239
GTATC 4949100 2.2623339 22.778059 200-209
GCCTC 7096550 2.2527986 74.61828 4
TGGTG 6131475 2.229652 33.95908 35-39
TACAT 4016480 2.218199 19.30813 190-199
GCCTG 7268970 2.217757 31.09618 210-219
TCCAA 4978595 2.2171583 41.001717 180-189
AATGA 4450235 2.2117155 26.042128 210-219
AAATG 4446030 2.2096257 33.35621 200-209
GCTCC 6842025 2.1719997 33.536766 80-89
CCTTC 5293950 2.1684723 55.27315 45-49
CAGTA 5046815 2.1600971 21.147972 45-49
GTGGT 5922200 2.153551 30.131086 120-129
GGGGG 9774140 2.1347773 94.00172 5
TATCA 3829110 2.1147196 17.21809 170-179
GTAGT 4809005 2.1127677 24.81711 120-129
GTGGG 7491995 2.1114004 29.920853 100-109
TCTAT 3542590 2.0895388 19.426031 110-119
GCAGA 6245255 2.0716054 23.679106 140-149
CCTCT 5053705 2.0700645 48.37069 35-39
TGCAG 5833155 2.0664983 43.115345 30-34
AACAG 5113835 2.049406 33.34515 210-219
CTCAC 5306745 2.0352967 54.92459 20-24
TGCAC 5521300 2.035199 26.635832 25-29
ATGGA 4938090 2.031332 12.810817 100-109
GCTGT 5364995 2.0299034 19.474014 230-239
CAGAG 6115720 2.0286376 32.358067 170-179
GCTAT 4424900 2.0227115 32.30825 50-59
TGTGA 4586175 2.0148704 18.424923 210-219
AGAGT 4834595 1.9887582 30.714006 150-159
TCTCT 3898685 1.9804199 33.675842 35-39
AACTA 3808965 1.9696419 35.636837 130-139
TGAGC 5538570 1.9621361 17.688091 200-209
TTCAG 4286815 1.9595902 47.634285 45-49
AGCAC 5663130 1.9545527 20.165945 130-139
GGCTC 6393235 1.9505708 19.856125 40-44
GGTCT 5130355 1.9411249 37.054302 15-19
ATATC 3510800 1.938925 13.996735 170-179
TCCGC 6088615 1.9328299 41.694992 70-79
CATAT 3498840 1.93232 13.724375 160-169
CTGTA 4192350 1.9164083 23.909307 190-199
CCGTG 6269125 1.9127048 20.126774 230-239
GATGG 5585430 1.9017527 21.726234 100-109
TCCAT 3992365 1.8988678 27.3015 45-49
GCACA 5498385 1.8976934 18.264904 130-139
TGGGA 5565530 1.8949773 17.352095 100-109
CAGAC 5486225 1.8934965 25.825735 140-149
CACAG 5471795 1.8885162 16.568386 140-149
TGGGG 6699685 1.8881108 121.99284 4
GGATC 5325840 1.886773 18.529823 70-79
GCCAG 6601725 1.8859228 35.001072 70-79
ACCAG 5444225 1.8790008 14.245891 190-199
TGGTA 4276010 1.8786038 30.326996 120-129
GTCAC 5096345 1.8785568 38.487522 160-169
ACTAT 3401430 1.8785229 17.03511 130-139
AGGAC 5661070 1.8778261 21.2802 220-229
ACCCT 4895465 1.8775582 43.266647 10-14
CCTGC 5893920 1.8710235 50.59856 20-24
CCAGA 5419585 1.8704966 33.922157 170-179
AGAGA 4828170 1.8596442 28.223257 170-179
CAGTT 4047540 1.8502127 24.657429 190-199
CTATT 3114790 1.8372079 17.923832 110-119
TAGTA 3440450 1.8261491 17.68447 120-129
GGTGG 6476205 1.8251295 18.195198 40-44
GAGGA 5722730 1.8244255 27.573042 220-229
TGTCT 3730835 1.8214244 49.931633 30-34
GCTGA 5114580 1.8119304 26.680733 200-209
CAAGG 5450665 1.8080333 17.715837 80-89
TTCCA 3779095 1.7974313 21.331451 180-189
TCAGC 4868080 1.7944163 12.296575 45-49
GCCGA 6251230 1.7857964 18.962456 220-229
TTCTG 3647235 1.7806103 43.915154 30-34
AGTCA 4148360 1.7755476 29.204567 160-169
AGTTC 3872450 1.7701753 21.59129 190-199
ACCTG 4783715 1.7633187 30.843248 25-29
TGACC 4747575 1.749997 14.116854 210-219
ATACT 3162575 1.7466092 26.046324 130-139
CCTGG 5723425 1.7462122 33.720707 2
GGATG 5116410 1.7420589 23.515427 100-109
GCAAA 4330475 1.735469 31.920158 200-209
TACAA 3345585 1.730025 16.962547 130-139
CTGAA 4025735 1.7230628 22.974834 200-209
CATGA 4009760 1.7162254 22.683054 60-69
TAGTG 3906335 1.7161927 25.859623 120-129
CTCAG 4584800 1.689997 80.86597 6
ATGCA 3939470 1.6861403 16.377834 60-69
AACAC 4004005 1.6695902 21.779015 190-199
ATTGG 3785645 1.6631693 20.019566 100-109
GTGCA 4693955 1.6629165 49.779873 25-29
GGAGC 6009725 1.6500119 13.420129 200-209
TCTGA 3574955 1.6341846 22.291838 220-229
AGGGG 6185345 1.6321586 33.17438 90-99
GGGGC 7148745 1.6245677 28.764425 90-99
CGATT 3528270 1.6128439 35.645954 160-169
GCCGT 5257280 1.6039919 29.3279 230-239
AGCTA 3735600 1.5988814 24.665724 50-59
TCTGC 4043440 1.5918095 33.03294 200-209
GGGCC 6727405 1.5907048 29.80824 2
TCTCA 3284140 1.5620185 25.41827 100-109
GGCTT 4117840 1.558029 22.11766 25-29
AGTGA 3782865 1.5561187 89.57961 9
CGTCC 4879280 1.5489266 18.729624 180-189
GTTCT 3159430 1.5424597 22.709877 190-199
GCACT 4174120 1.5386165 25.577667 25-29
CTCCC 4643010 1.5335879 13.633759 190-199
AGTTA 2874270 1.5256275 16.112518 50-59
GAGCA 4566780 1.5148406 8.079736 200-209
CAGCT 4109175 1.5146773 12.103157 50-59
CGGCC 6147685 1.512473 31.589745 230-239
CCATG 4102380 1.5121726 16.036514 160-169
TTCTC 2975175 1.5113034 21.277597 190-199
ACAAT 2888960 1.4939013 21.74366 180-189
CATCA 3344985 1.4896495 31.710386 45-49
GTCCG 4877675 1.4881747 34.140663 70-79
TTAGT 2561655 1.4521657 24.723671 110-119
CTGGT 3806070 1.4400675 31.686087 35-39
GTTAC 3150270 1.4400523 14.978358 50-59
GTGAC 4057310 1.4373738 14.6524515 210-219
ACTCC 3694985 1.4171382 16.3726 140-149
TACGC 3840890 1.4157852 24.24191 140-149
CTCAA 3153845 1.4045275 19.02618 150-159
CTTTA 2380095 1.4038602 40.145763 45-49
GGCCT 4598730 1.4030687 71.48209 3
GGCAG 5097205 1.3994731 14.103094 150-159
GTCCA 3768320 1.3890353 28.66837 180-189
ATACA 2675995 1.3837755 23.48671 35-39
CGACA 4006040 1.3826306 17.060299 70-79
GGGGT 4903755 1.3819803 116.53032 7
GAACT 3220070 1.3782285 15.1340475 190-199
AGCAG 4130855 1.3702404 12.609664 50-59
AACCC 3805745 1.3666728 20.057411 140-149
TTATT 1866905 1.3655788 21.910902 240-247
CAACC 3801730 1.365231 15.588246 140-149
CGTGT 3607590 1.3649704 23.945076 230-239
CAACT 3056610 1.3612251 16.660608 130-139
CCATA 3029600 1.3491964 14.142896 160-169
ATCTA 2439685 1.3473756 20.228664 110-119
ACAGA 3351395 1.3430958 21.092258 140-149
ACATG 3134830 1.3417449 15.178863 190-199
GTGCG 4561225 1.3374839 17.366693 240-247
AAGCT 3123955 1.3370901 15.974869 200-209
CTACG 3626180 1.3366412 16.243038 130-139
ACAGT 3122015 1.33626 12.258355 120-129
GAGAG 4184835 1.3341393 25.48066 220-229
TGGAA 3216855 1.3232851 18.991936 120-129
TCACT 2781720 1.323055 13.9847355 190-199
CACGT 3585005 1.3214638 16.957695 180-189
ATTAT 1926925 1.3197292 9.869618 50-59
TCAAG 3072255 1.3149619 23.094189 150-159
TAGCA 3068225 1.3132371 10.985568 130-139
CCTAC 3423550 1.3130345 17.946 190-199
TGCGA 3669960 1.3001482 14.335875 240-247
TGTCC 3302260 1.3000239 54.12153 15-19
AAGAG 3372700 1.2990475 18.560799 150-159
TGTGT 2754945 1.2926592 15.362535 240-247
ACACA 3096305 1.2910976 7.9409904 130-139
GTGGC 4376550 1.2833318 14.303829 40-44
CCAGT 3466975 1.2779568 18.616982 190-199
AACCA 3037875 1.2667334 17.886122 190-199
TTACA 2289790 1.2645924 9.384976 120-129
CTTCT 2477250 1.2583718 23.745905 30-34
TCCGT 3192915 1.2569773 13.141262 150-159
CAATT 2275230 1.2565514 22.431007 180-189
TGGAC 3542090 1.254848 16.513912 80-89
AGACC 3623975 1.250766 99.005554 9
CCTTT 2460680 1.2499545 46.112385 45-49
GATTG 2819485 1.2387004 23.516891 100-109
AGAGC 3728130 1.2366531 24.123653 220-229
ATATT 1804390 1.2358063 24.808891 240-247
CGGCT 4041450 1.2330431 21.512718 230-239
ACGTC 3333925 1.2289135 18.762714 180-189
ACATA 2366090 1.2235216 20.024292 130-139
TGAGT 2783320 1.2228118 21.837782 90-99
AATTC 2212235 1.2217606 22.377674 180-189
AGTCG 3447410 1.2213058 15.500491 150-159
ATCCG 3308125 1.2194033 23.750088 70-79
TACCA 2681800 1.1943078 10.557805 45-49
AAGGC 3595425 1.1926337 29.912725 25-29
GTCGA 3345640 1.1852521 12.067712 150-159
GGGTG 4180080 1.1780336 15.20634 60-69
GAAGT 2861725 1.1771988 14.319774 140-149
ATCAT 2124390 1.1732463 15.375739 110-119
GATCC 3174450 1.1701295 23.59757 70-79
AGGCC 4089875 1.1683593 14.538839 2
GATCT 2546940 1.1642579 9.688346 220-229
TCGGA 3259105 1.1545955 92.4748 5
CTTCG 2922400 1.1504818 103.60336 3
GGACT 3226845 1.1431669 12.557655 90-99
AGGTC 3221710 1.1413475 18.849733 10-14
ACGCA 3299860 1.1389021 20.470985 140-149
ACAAG 2840635 1.138405 18.157627 80-89
ATTAG 2142410 1.1371652 24.6443 110-119
TCGAG 3191470 1.1306347 11.6449795 150-159
TTGGG 3104755 1.1290143 14.747845 100-109
GCAAG 3402045 1.128488 17.559843 25-29
CTTGA 2453185 1.1214007 23.045572 90-99
TTGAG 2548495 1.1196449 21.531809 90-99
GAACC 3240235 1.1183233 10.542055 190-199
AGCTC 3031205 1.1173283 12.461704 210-219
ACACC 3106395 1.1155308 13.510302 40-44
GCTTC 2821360 1.1107047 27.228508 2
GACCC 3725225 1.1072685 42.65225 10-14
ATGGG 3230285 1.0998623 20.693071 100-109
CCGAT 2983520 1.0997511 23.843298 160-169
ATCAA 2121580 1.0970837 12.329867 110-119
TTCGG 2894685 1.0952353 99.65823 4
CGCTG 3583185 1.0932269 11.540905 190-199
GCCCC 4254110 1.0889771 12.5141945 80-89
ATTCC 2284690 1.0866554 20.819326 180-189
CAGCA 3126440 1.0790485 13.090172 45-49
AAAAA 1912630 1.0752938 5.1859717 190-199
AGATC 2500025 1.0700406 13.029608 210-219
GCACC 3594580 1.0684363 11.168766 130-139
TAGAC 2493560 1.0672735 16.764385 170-179
CGCAG 3729930 1.0655336 15.967399 140-149
GACAG 3197735 1.0607165 16.411098 70-79
CACAT 2374350 1.0573887 13.099621 130-139
CCCCT 3198355 1.0564178 9.7272835 70-79
GTATA 1983080 1.0525947 9.674906 240-247
GGGAG 3982750 1.0509487 14.540352 5
TACAG 2451165 1.0491279 8.816204 120-129
GGAGA 3288525 1.0483929 82.86027 7
GAAGC 3147775 1.0441443 12.51476 200-209
CTATA 1887285 1.0422993 13.528479 50-59
TGAGG 3056525 1.0406996 12.450237 220-229
TTTCA 1758970 1.0374997 7.873147 100-109
TGGCT 2732940 1.0340372 31.13862 40-44
ATGAG 2495700 1.0266308 19.642115 60-69
ATGGT 2331835 1.0244584 9.507822 120-129
ACCGC 3441145 1.02283 11.466993 220-229
ATGAC 2381085 1.0191329 11.460193 160-169
GCTCT 2575840 1.014049 16.845554 210-219
GCCAA 2936175 1.0133812 15.45144 180-189
GAGCC 3537365 1.0105232 17.615454 220-229
AAGGT 2456315 1.0104293 21.387623 15-19
AACTC 2268175 1.0101049 17.01889 190-199
CTCCG 3181125 1.0098475 9.5905695 140-149
AAACT 1944815 1.0056772 16.971678 130-139
CGAGG 3661965 1.005418 14.555624 220-229
CCGTC 3161300 1.003554 10.611452 140-149
ACTCA 2250505 1.0022358 12.898912 190-199
TAGTT 1753815 0.9942127 14.385242 50-59
TTATA 1440530 0.9866026 12.622905 110-119
GATAC 2305050 0.9865889 25.445059 35-39
CGTGA 2784425 0.9864318 19.70918 150-159
AGCCG 3450410 0.9856826 18.669691 220-229
ACAGG 2961710 0.98242515 15.940193 70-79
GTAGC 2758905 0.97739077 11.086937 50-59
ATGAT 1840625 0.9769813 8.53876 120-129
GTAGA 2352770 0.96783507 16.568806 170-179
CGAGT 2729510 0.9669772 15.389726 160-169
AACTG 2258875 0.9668255 12.708672 60-69
TTTAT 1319025 0.9648228 11.617797 240-247
GCAGT 2708255 0.9594472 6.828245 50-59
CGGAC 3354700 0.9583411 10.696569 220-229
CAGAA 2380465 0.95398843 18.760921 140-149
AAGTT 1794855 0.95268714 18.53346 140-149
GGGAC 3468415 0.95227754 9.628235 90-99
TACAC 2138170 0.9522087 22.964352 35-39
CCGAG 3313075 0.94645005 17.517445 220-229
TAACA 1829875 0.946241 9.226164 120-129
ACCAA 2259420 0.9421331 16.27842 130-139
GAGAT 2266740 0.93244576 12.643709 210-219
TATTT 1267165 0.9268891 11.686591 240-247
AGCCC 3106735 0.92343134 11.664189 80-89
CATAC 2064575 0.9194341 13.395216 130-139
TTTAG 1619790 0.91823584 28.433912 45-49
GGTAT 2081760 0.91459155 8.957776 110-119
CCAAC 2542850 0.9131573 13.250553 130-139
TCATA 1650235 0.91138256 8.663836 110-119
AATCA 1756540 0.9083189 13.344546 110-119
GCCAT 2456465 0.90547425 7.33492 60-69
GTTAT 1592610 0.9028279 10.094943 110-119
TGGTT 1918765 0.9003117 9.16848 50-59
CCCAG 3026315 0.89952767 14.531404 80-89
GATGA 2185465 0.8990125 6.948042 60-69
TATGG 2037460 0.895129 11.96106 35-39
AGTCT 1951765 0.89219135 15.939851 8
AGAAG 2305895 0.88815105 16.411732 140-149
GTTTC 1815445 0.8863152 5.566151 100-109
CCCCA 2830310 0.87532324 16.695984 80-89
GCCTA 2373430 0.8748668 17.27711 190-199
TCCTT 1712030 0.86966205 10.732454 1
CATGG 2452790 0.8689442 8.885908 200-209
GACTG 2444620 0.86604977 12.151311 90-99
ATAGT 1627935 0.86408806 15.834257 120-129
CGGAG 3142255 0.86272794 71.60119 6
GTCTG 2266065 0.85739005 14.101802 3
TTATG 1507740 0.8547163 12.655316 50-59
GTCTA 1860340 0.8503992 9.650134 30-34
GATCA 1975205 0.8454113 9.35554 110-119
TTACC 1766930 0.84039575 9.693595 45-49
ATCCA 1879055 0.8368149 9.844544 180-189
GGTGC 2851870 0.83625126 12.14864 60-69
CTATC 1757630 0.8359724 8.336058 110-119
CGTAT 1825470 0.83445936 6.8988075 240-247
TCTTC 1641955 0.834066 45.453785 2
GCTTG 2203365 0.8336668 17.490425 90-99
TCATC 1726975 0.8213922 10.5940275 110-119
ATATA 1264205 0.81070507 11.174001 130-139
ACGCT 2187720 0.8064124 14.204997 190-199
ACATC 1808720 0.8054921 5.6554136 110-119
GGTTC 2121950 0.8028626 10.580787 160-169
TGGCA 2255495 0.79904896 5.4788923 100-109
ACAAA 1649930 0.79886115 12.80858 130-139
GGTAG 2337495 0.7958811 11.448934 120-129
CATTA 1433725 0.7918097 12.676274 110-119
TGATG 1783620 0.78360796 11.958649 120-129
ATATG 1475475 0.7831642 5.652177 50-59
TATAC 1417810 0.7830202 9.303657 130-139
CCTCC 2364285 0.78092414 10.0000105 140-149
CTGAC 2100615 0.77430487 9.630972 220-229
TCAAC 1725550 0.76845324 7.6358557 110-119
GGGAT 2226795 0.75818944 13.7703905 100-109
CCAGC 2549935 0.7579308 6.7594385 80-89
TGACT 1655350 0.756694 5.0212665 2
TATGA 1420390 0.75392574 5.4553523 50-59
GCGGA 2708860 0.7437363 8.95233 220-229
ATCTT 1259785 0.74306357 12.475227 3
TTGGA 1689745 0.7423653 6.569394 60-69
AACGC 2147295 0.74110985 15.09767 180-189
ACGCC 2490140 0.7401576 13.523603 180-189
GGTTA 1672400 0.734745 8.092116 50-59
GACCA 2118920 0.73131657 6.333064 170-179
GGATA 1776610 0.7308261 16.386576 35-39
TATTG 1287225 0.72970957 6.9099765 240-247
GACCG 2552945 0.72930276 8.940657 210-219
GTTCA 1595120 0.72916174 12.913339 160-169
TTTCT 1154490 0.72726774 9.452956 4
CAACG 2106365 0.72698355 15.4376745 180-189
GGGCA 2645805 0.72642416 14.082446 150-159
TTTAC 1226685 0.72354007 13.251595 45-49
CCCCC 2693520 0.71740466 13.225447 80-89
GATTA 1347255 0.71510655 5.7629147 50-59
ATAAA 1169545 0.70224345 7.427788 110-119
GAAAT 1411370 0.7014346 7.1474624 110-119
CGCCG 2847085 0.7004489 10.9248495 220-229
CACAA 1673930 0.6979955 8.426047 130-139
CATCC 1819790 0.69794434 6.575894 110-119
TATAG 1309490 0.6950614 6.7700324 120-129
TCCGG 2266955 0.69164616 11.342097 70-79
CAGTC 1866030 0.6878348 5.7260113 210-219
ATGCC 1852460 0.6828328 7.697387 50-59
AATAC 1314490 0.6797318 11.001383 130-139
CCCGT 2136330 0.6781775 10.368021 140-149
TGCCA 1836055 0.67678577 7.035766 60-69
GCGAC 2361070 0.6744897 7.49749 70-79
GCCGC 2739395 0.67395467 12.113665 220-229
CAAAC 1606950 0.67006624 13.614672 130-139
AGATG 1597620 0.65719676 5.990977 120-129
GACGA 1979785 0.6567119 9.981573 220-229
AGGCA 1974520 0.65496546 6.2079854 80-89
TACTT 1103295 0.65076053 10.942908 1
ACTTC 1366015 0.6497107 30.294928 4
AAATA 1079750 0.64832675 12.20983 130-139
TCAAT 1138060 0.62852144 8.129494 110-119
CACCG 2108175 0.6266241 9.022274 45-49
CCCTA 1626970 0.6239919 12.292459 110-119
AAGCA 1555730 0.62346995 5.786671 120-129
TCCAC 1618430 0.6207167 10.405573 180-189
CACGC 2080355 0.6183551 11.511349 190-199
CACGA 1750245 0.6040735 8.894526 180-189
ACCTA 1355170 0.6035089 7.8589478 130-139
AATAA 1004455 0.6031165 6.795564 130-139
GTTCC 1529755 0.6022294 7.3350296 140-149
AATGG 1460960 0.6009803 6.6572046 120-129
GGCCC 2442615 0.60093987 10.388061 70-79
ACGAC 1731620 0.5976453 9.28196 220-229
CCGGC 2425060 0.596621 9.165478 70-79
GGTGA 1751100 0.5962226 6.690413 120-129
ATTTC 1005915 0.5933226 6.824614 240-247
CTTTG 1214465 0.5929119 7.368884 45-49
ACCCG 1993625 0.5925758 9.046299 140-149
TGATT 1040585 0.5898928 6.2342467 50-59
TAGCT 1285360 0.5875642 12.113788 50-59
GGGGA 2191015 0.5781544 9.370872 4
AGTAA 1161160 0.5770831 5.319823 50-59
TTCAA 1035900 0.57210106 5.557397 200-209
ATTAA 882680 0.566042 6.8584886 110-119
TCAGG 1564295 0.55417913 5.420975 150-159
CCGTA 1502300 0.5537607 5.9298267 230-239
GGAGG 2097140 0.5533831 5.327168 6
GAAGA 1416890 0.545737 6.4801464 15-19
CTGTT 1109935 0.5418794 5.908425 240-247
CCACG 1815960 0.53976744 7.804523 180-189
CGGCA 1884395 0.5383173 10.578753 70-79
ACATT 973380 0.5375729 6.68592 110-119
CCTAT 1125215 0.5351801 5.106333 110-119
AAGAT 1076750 0.53513235 5.9382534 15-19
TAATA 831960 0.53351647 7.392422 130-139
CTGCG 1745720 0.53261775 11.758932 25-29
GCTAC 1440845 0.5311079 7.8806973 50-59
GTCCT 1343825 0.52903306 6.206313 45-49
TTAGC 1147940 0.52474666 8.860765 45-49
TATGT 925565 0.5246896 6.878038 140-149
CGGTT 1384870 0.52398044 10.458142 160-169
AAATC 999235 0.5167113 7.618978 110-119
GGGTT 1412300 0.5135693 6.4184427 100-109
AGTAT 966545 0.51303023 5.721966 110-119
GTAAC 1170350 0.50092375 7.2076406 120-129
TGACG 1402235 0.49676648 8.781682 220-229
GGTTT 1056160 0.49556524 5.965674 100-109
CGCGG 2093685 0.49505496 6.527816 220-229
ACCCC 1587570 0.490984 8.524363 140-149
TATCC 1031635 0.49067125 5.4179764 110-119
TGGCC 1601925 0.48874605 15.395327 2
CATAA 943630 0.48795763 6.0990663 110-119
AACAT 924540 0.478086 5.956026 110-119
CAATC 1031800 0.45949993 6.00589 110-119
CCGCG 1852075 0.45565334 6.7357526 220-229
ATGCT 976145 0.4462157 6.902765 50-59
GCCTT 1124325 0.44262096 9.325733 1
ATTCT 744120 0.43890706 7.0589976 2
TGCTA 952125 0.43523565 6.11254 50-59
TGCGC 1420720 0.43346053 8.659397 25-29
CCGGT 1416575 0.43219584 8.097118 160-169
GGTCA 1212765 0.42964342 5.527275 160-169
TTGGT 905195 0.4247303 5.166745 120-129
TAAAT 657770 0.4218125 7.267618 130-139
CTAAC 936415 0.41702133 5.8436027 110-119
CACTT 872820 0.41513485 7.040942 3
GCATG 1167855 0.41373327 8.22565 60-69
CCTAA 918025 0.40883157 8.097694 110-119
ATGGC 1135065 0.40211684 7.4451704 1
TTTGA 708870 0.4018483 6.2708893 45-49
ACGAG 1210575 0.40155828 7.652571 180-189
TACCT 839480 0.3992775 5.112908 2
AGCTT 862325 0.3941862 7.0786695 1
GCGCT 1290955 0.3938693 7.8330045 25-29
CCGGG 1658345 0.39211813 5.3438888 80-89
CATGC 1049340 0.3867958 6.869342 60-69
GGTAA 933515 0.3840106 7.60158 120-129
GAGGC 1392210 0.38224092 5.902957 35-39
TTCTT 571015 0.35970932 19.08582 1
CGGTG 1225775 0.3594329 5.4276304 8
TGGGC 1176990 0.3451277 9.472346 1
ATGTG 778100 0.34184715 5.2094917 140-149
TGCTG 898135 0.339819 14.027416 1
CGAGC 1159005 0.33109432 6.419925 180-189
CTCTT 622495 0.31620955 8.691882 1
TCACA 695750 0.30984402 6.1365337 5
GCTTT 629550 0.3073515 5.1262794 45-49
GCGAG 1111480 0.30516458 5.558089 240-247
GACTT 661630 0.30244446 6.848285 1
CGGGA 1094835 0.30059457 5.124343 80-89
CTCGG 984065 0.30023745 5.9110875 6
AACTT 531775 0.2936857 10.780996 3
ATTGT 517350 0.29327834 5.7141175 240-247
CGCTT 742665 0.29237017 5.7504716 1
GAGGT 853890 0.29073638 6.6922574 7
TTCCT 562110 0.28553572 6.3601074 4
CCCTT 678530 0.27793488 5.7166295 3
GTCTT 547710 0.26739654 11.970928 1
AATCT 477125 0.2635039 7.3339553 2
TAACT 476860 0.26335758 7.2254915 2
TTGGC 695605 0.2631896 7.4126153 1
TGTAA 485855 0.2578859 7.9926853 25-29
CGGGG 1118510 0.25418383 9.4347 3
CCTCG 774780 0.24595372 6.441647 5
TGCTT 431610 0.21071556 11.027728 1
TCGGT 556450 0.21053883 7.0226054 7
TGCCT 477760 0.18808316 5.031197 4
TCGCA 450180 0.16594024 5.599412 7
TAGGC 437605 0.15502931 7.2119207 1
TTGCT 248690 0.12141251 5.7598705 4
Produced by FastQC (version 0.10.1)