FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_17_SL248346.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_17_SL248346.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6727053
Filtered Sequences 0
Sequence length 251
%GC 54

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 31134 0.4628178193333693 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24444 0.3633686251617164 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22647 0.3366555904940841 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22429 0.33341494410702577 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21420 0.3184158055540814 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20581 0.3059437765690266 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 19841 0.2949434172735074 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19339 0.2874810113730336 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18748 0.27869558928701765 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18463 0.27445896442320283 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 17954 0.26689250106993356 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17904 0.26614923354996606 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17657 0.2624774920013266 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17536 0.2606787846030052 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17209 0.2558178150224177 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16909 0.2513582099026126 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16644 0.24741889204678483 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16463 0.24472826362450245 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16462 0.24471339827410307 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16400 0.24379174654934338 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15916 0.23659691695605786 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15831 0.23533336217211312 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15714 0.23359411617538914 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15252 0.22672632429088935 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15198 0.22592359536932444 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14859 0.2208842415839447 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13877 0.2062864674917828 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13861 0.20604862188539322 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13662 0.20309041715592252 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 13556 0.2015146900135914 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13510 0.20083088389522127 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 13348 0.19842269713052654 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13029 0.19368065035313384 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12987 0.19305630563636111 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 12866 0.19125759823803976 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12734 0.18929537198532553 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12578 0.18697637732302688 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 12343 0.1834830199791796 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 12031 0.17884503065458232 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 12013 0.17857745434739403 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11906 0.17698686185466356 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 11116 0.1652432350391769 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11088 0.1648270052279951 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11032 0.16399454560563148 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 10916 0.16227016495930685 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10914 0.16224043425850815 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 10882 0.16176474304572894 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 10791 0.16041199615938806 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 10447 0.1552983156220116 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 10330 0.15355906962528762 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 10270 0.1526671486013266 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10017 0.148906214950291 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 9741 0.14480337824007036 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9617 0.14296007479055092 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9517 0.1414735397506159 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 9408 0.13985321655708674 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9294 0.1381585666115608 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9211 0.13692474252841474 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 8982 0.13352057728696357 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 8884 0.13206377294782723 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 8666 0.12882312656076889 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 8238 0.122460756589847 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7982 0.11865522688761333 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7518 0.11175770430231484 No Hit
TTGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 7354 0.1093197868368214 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7116 0.10578183344177607 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6973 0.10365608833466898 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 6937 0.10312093572029239 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6761 0.10050463405000674 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 13506670 7.5854187 65.97461 35-39
TATTA 6722890 7.2010894 125.08251 240-247
TGGAT 10200985 7.0703077 77.95608 35-39
TCACC 10470360 6.4257493 74.44701 160-169
TCTCC 9318200 6.1589856 87.12166 20-24
CTGTG 9750020 5.8972793 86.95185 25-29
TCCCT 8785120 5.8066397 73.20123 15-19
AGTGG 10520335 5.6519117 39.36765 100-109
CTGAG 9993560 5.6124372 50.1647 15-19
TGTAT 6138755 5.489174 58.676517 240-247
TTACT 5641915 5.27374 111.32115 240-247
ATTAC 5689250 4.9377856 104.00847 240-247
TGGGT 8473415 4.902744 45.49717 100-109
CCTGA 8286455 4.864805 51.938667 15-19
CTCTG 7435985 4.7016487 73.43941 35-39
TTCAC 6154890 4.6617336 74.12842 40-44
CTCCA 7484480 4.59329 41.41344 80-89
ACACG 8391600 4.5743194 48.63423 230-239
GACAC 8387195 4.5719185 46.124508 230-239
TGAGA 6846200 4.405861 93.00875 15-19
TCTGG 7142925 4.3203835 134.5042 35-39
ATTCA 4934060 4.2823443 110.62189 40-44
TACTG 5865615 4.249866 78.355896 240-247
AAGGG 8484865 4.2324877 38.724262 90-99
CCCTG 8176755 4.189167 85.19325 10-14
AGCCT 7102575 4.169774 50.78435 30-34
CAGCC 8740980 4.15807 55.605347 30-34
CCAGG 9031255 4.10974 53.119354 80-89
TGGAG 7621715 4.0946655 38.222767 90-99
GAAGG 8195200 4.087995 30.611399 150-159
ACCAT 5728865 4.028847 66.442375 160-169
GAGAC 7680520 4.00504 85.494896 8
TCAGT 5507805 3.990619 96.49302 7
TCCAG 6783985 3.9827366 40.675938 80-89
GGGTC 8397035 3.9367847 125.23522 8
CACCA 6875765 3.9180338 56.845657 160-169
ACTAC 5554065 3.9059184 35.308098 130-139
GTATT 4363240 3.9015374 67.9333 240-247
TACTA 4416815 3.8334198 28.384653 130-139
CTGGG 8116275 3.8051565 132.82141 3
CACCT 6164535 3.7832274 44.249657 40-44
GACTC 6398575 3.7564704 52.26086 20-24
ACTGT 5139945 3.72409 67.68301 240-247
GATTC 5108405 3.7012382 59.09825 40-44
AGACT 5493750 3.6958609 68.02222 15-19
TGAAG 5714265 3.677406 39.14782 150-159
GGACA 6921085 3.6090295 28.179197 220-229
GCTGG 7629545 3.5769627 33.509487 2
CAAGA 5663850 3.5378833 43.104424 180-189
CCTGT 5555760 3.5128136 62.59175 25-29
ACTGG 6202375 3.4832866 43.541176 60-69
ACTCT 4532070 3.4326046 68.72352 20-24
GAGTG 6349095 3.4109678 38.620026 90-99
TCCTG 5324325 3.3664815 64.39095 20-24
TATCT 3553560 3.3216648 48.682163 200-209
CTCCT 4999775 3.3046665 94.5123 20-24
GGTCC 6650210 3.2592416 143.03105 9
CTGCA 5482065 3.2184064 44.78698 25-29
GGATT 4640580 3.2163885 56.289383 40-44
AGACA 5144995 3.2137842 37.100193 170-179
AGCTG 5721885 3.213441 36.705425 60-69
CATCT 4168555 3.157277 33.162315 170-179
GTCTC 4965155 3.139384 32.962746 30-34
CCATC 5042465 3.0946038 34.37457 45-49
GTCCC 5987045 3.0673203 75.62976 10-14
CCTCA 4908305 3.0122688 82.29579 5
GGAGT 5492545 2.950797 36.571545 90-99
GGCTG 6268175 2.938711 34.55098 90-99
AGGGA 5762055 2.874274 39.594234 80-89
AAGAA 3996015 2.8602822 36.05972 180-189
GTGTA 4120745 2.85609 35.97223 230-239
ATCTC 3745855 2.8371224 48.079628 170-179
TGTGC 4660730 2.819033 57.16188 25-29
AGTAG 4371825 2.8134813 28.070787 50-59
GAGCT 4998950 2.8074372 29.542728 60-69
GTGAA 4322445 2.7817025 38.81922 10-14
ATCTG 3823605 2.7703502 36.508297 200-209
GGAAG 5494255 2.7406878 29.625153 80-89
GACAA 4353095 2.7191296 24.407766 180-189
CAGGG 6240505 2.7165673 35.99235 80-89
ACCTT 3550160 2.6889029 71.67079 40-44
CTATG 3698435 2.67966 28.16865 50-59
GAACA 4273125 2.669177 34.33809 210-219
GGGAA 5234150 2.6109402 33.22184 80-89
ATGAA 3350450 2.582854 27.252182 210-219
CACGG 5650760 2.5714202 44.080353 230-239
GAGTC 4525680 2.5416462 27.61429 150-159
TGCAA 3766015 2.533546 35.206257 200-209
CCCTC 4729215 2.5328016 27.34546 20-24
CGCCA 5316530 2.529065 39.818268 70-79
AGGGC 5755030 2.5052342 32.627735 150-159
AGAAC 3978065 2.4848697 29.716045 190-199
CAGTG 4407235 2.4751265 74.737495 8
AGGCT 4386420 2.463437 20.279245 25-29
TATAT 2296025 2.4593415 24.313623 240-247
ACGGC 5382140 2.4491827 48.711338 230-239
ATCAG 3630160 2.4421508 26.024216 45-49
CTACA 3464275 2.4362652 19.728374 190-199
CCAAG 4464650 2.4337115 38.09437 180-189
CTCTC 3603665 2.3818893 98.57789 20-24
CACTG 4050935 2.3782198 19.266504 60-69
GTGGA 4426440 2.3780463 26.030552 100-109
TCTGT 3044285 2.375538 24.397137 210-219
GCAGC 5215835 2.3735044 34.36519 30-34
CCGCC 5688945 2.3616383 34.414722 70-79
AAAAA 2744895 2.3535476 18.8388 90-99
CTGTC 3715805 2.3494413 47.909912 30-34
GTATC 3226955 2.3380542 22.954184 200-209
TGAAC 3473405 2.3366957 37.37759 210-219
ACAGC 4285310 2.3359523 26.802156 210-219
GGGCT 4951400 2.3213665 42.80232 90-99
CTACT 3058530 2.3165405 22.698692 50-59
GTGGT 3987545 2.3072057 31.429276 120-129
TATGC 3182750 2.306026 21.209469 50-59
CTTCA 3041240 2.303445 50.142654 45-49
GGCCG 5997590 2.2783868 25.397158 230-239
CAAAT 2824110 2.275851 34.430206 200-209
CAGGC 4990825 2.271112 33.42049 70-79
TCCAA 3216895 2.262294 42.48273 180-189
TGGTG 3907430 2.260851 33.43991 35-39
GCCTC 4410145 2.2594335 72.882545 4
GCCTG 4501565 2.2061992 31.143482 210-219
GCTCC 4302745 2.2044096 34.3247 80-89
TATCA 2535010 2.2001731 16.594778 170-179
ACAAC 3341825 2.1821315 19.486904 180-189
AATGA 2828785 2.1807036 25.760057 210-219
AAATG 2816315 2.1710906 32.577106 200-209
GGGGG 6233505 2.1669657 94.59417 5
CCTTC 3256525 2.1524425 55.650085 45-49
CAGTA 3197120 2.150828 21.237438 45-49
TACAT 2454255 2.1300845 18.377033 190-199
GTGGG 4698745 2.1073277 30.08465 100-109
CCTCT 3182250 2.1033494 49.310883 35-39
GCTAT 2902740 2.103148 33.9336 50-59
GTAGT 3015230 2.0898573 24.799595 120-129
GCAGA 3997365 2.084443 24.36356 140-149
TGCAG 3702170 2.0791583 43.398712 30-34
GCTGT 3435810 2.078143 19.343563 230-239
TCTAT 2185610 2.0429835 19.792522 110-119
CTCAC 3311930 2.0325596 54.9249 20-24
AACAG 3227395 2.015969 32.883106 210-219
TGCAC 3429340 2.013294 26.000479 25-29
AGAGT 3115060 2.0046923 29.869503 150-159
CAGAG 3825190 1.9946618 32.255016 170-179
AACTA 2470630 1.9909939 36.093487 130-139
GGCTC 4027930 1.9740727 19.7675 40-44
AGCAC 3599795 1.9622734 20.189697 130-139
TGAGC 3493985 1.9622405 17.762732 200-209
TGGTA 2820905 1.9551705 31.030647 120-129
ATGGA 3036800 1.9543278 11.962406 100-109
GGTCT 3229170 1.9531568 36.439713 15-19
TCCGC 3810055 1.9519916 42.274452 70-79
CCGTG 3979685 1.9504277 20.25115 230-239
TGTGA 2813085 1.9497507 18.616865 210-219
TTCAG 2666410 1.9319178 47.070137 45-49
TCTCT 2368330 1.931899 32.412643 35-39
ATATC 2223635 1.9299262 13.356508 160-169
CTGTA 2663535 1.9298347 24.073137 190-199
CAGAC 3521135 1.9193952 26.657415 140-149
GGTGG 4248835 1.9055488 17.898111 40-44
CATAT 2192145 1.9025955 14.164081 160-169
GCACA 3471240 1.8921969 18.317123 130-139
TGGGG 4195665 1.8817028 122.928894 4
GCCAG 4123995 1.8766547 35.4606 70-79
ACTAT 2154830 1.8702093 16.713116 130-139
ACCCT 3040520 1.8659929 43.385708 10-14
CCAGA 3421540 1.8651052 33.92519 170-179
AGGAC 3564490 1.8587186 20.708088 220-229
CCTGC 3622945 1.8561298 50.115185 20-24
GGATC 3303525 1.8552772 17.636194 70-79
GTCAC 3155595 1.852584 37.910793 160-169
AGAGA 3089235 1.8459386 27.671122 170-179
GATGG 3419840 1.8372641 20.834105 100-109
CTATT 1964170 1.835994 19.51029 110-119
TCCAT 2419125 1.8322532 26.171303 45-49
TTCCA 2418480 1.8317647 23.098755 180-189
ACCAG 3359245 1.8311477 13.63691 190-199
CAGTT 2518560 1.8247948 24.348764 190-199
CACAG 3345520 1.8236661 15.973501 140-149
TGGGA 3362065 1.806225 16.647377 100-109
TGTCT 2310860 1.8032265 48.942783 30-34
TCAGC 3071455 1.8031874 11.605758 45-49
GCTGA 3209080 1.8022367 26.919155 200-209
GAGGA 3605155 1.7983521 26.837988 220-229
GCCGA 3909485 1.7790403 19.396793 220-229
ATACT 2042230 1.7724819 26.357584 130-139
CAAGG 3388795 1.7671018 17.220276 80-89
TAGTG 2536965 1.7583717 25.757 120-129
TTCTG 2231585 1.7413661 43.09049 30-34
AGTCA 2585475 1.7393503 28.749926 160-169
ACCTG 2960190 1.737866 30.566446 25-29
TAGTA 2084575 1.7307296 16.651201 120-129
GCAAA 2766795 1.7282587 31.60428 200-209
AGTTC 2385030 1.728047 21.621935 190-199
CCTGG 3502485 1.7165539 33.362812 2
TGACC 2912055 1.7096068 14.287531 210-219
TACAA 2118625 1.7073253 17.270916 130-139
CATGA 2537030 1.7067595 23.149319 60-69
CTCAG 2907100 1.706698 78.386246 6
CTGAA 2499805 1.6817168 22.60946 200-209
GTGCA 2981405 1.6743728 49.70671 25-29
GCCGT 3416335 1.6743324 29.52024 230-239
ATGCA 2487965 1.6737516 16.2028 60-69
AGCTA 2486665 1.672877 25.648466 50-59
AACAC 2559475 1.6712757 22.603657 190-199
GGATG 3076155 1.6526235 22.21444 100-109
GGAGC 3773895 1.642822 13.144114 200-209
TCTCA 2138125 1.6194228 26.208517 100-109
TCTGC 2551025 1.6129704 33.440178 200-209
AGGGG 3862880 1.6085943 32.77199 90-99
ATTGG 2316495 1.6055639 20.102139 100-109
CAGCT 2725060 1.5998261 13.06609 50-59
GGGGC 4387340 1.5943604 28.808094 90-99
GGGCC 4176205 1.5864724 28.71465 2
TCTGA 2179100 1.5788428 21.434877 220-229
CGATT 2173775 1.5749846 34.77285 160-169
ACAAT 1933620 1.5582365 23.115795 180-189
CTCCC 2901180 1.5537704 13.946591 190-199
GTTCT 1972985 1.5395736 23.021732 190-199
GGCTT 2527760 1.5289105 21.574396 25-29
GCACT 2599720 1.5262415 24.900948 25-29
CGTCC 2977135 1.5252646 18.946966 180-189
CGGCC 3825255 1.5190657 31.837612 230-239
CCATG 2584140 1.5170949 15.472445 160-169
TTCTC 1857560 1.5152524 21.951086 190-199
AGTGA 2340175 1.5060161 85.21877 9
GTCCG 3066865 1.5030583 34.562527 70-79
AGTTA 1795575 1.490786 15.710113 50-59
GAGCA 2856450 1.4895083 7.7572913 200-209
TTAGT 1646610 1.4723716 25.254068 110-119
TACGC 2506105 1.471282 24.773312 140-149
CTGGT 2421265 1.4644972 30.879316 35-39
CTTTA 1557530 1.45589 41.561256 45-49
ACTCC 2372085 1.4557688 17.19841 140-149
CATCA 2054635 1.4449302 30.375067 45-49
GTTAC 1980020 1.4346014 15.718468 50-59
CGTGT 2363930 1.4298183 24.021746 230-239
AAGAG 2353510 1.406314 18.186201 150-159
GTGAC 2501935 1.4051 14.951809 210-219
GGGGT 3124325 1.4012203 117.380806 7
GGCAG 3202030 1.3938824 13.636756 150-159
CTACG 2372550 1.3928748 16.503874 130-139
CTCAA 1965365 1.3821508 18.074577 150-159
TAGCA 2044375 1.3753312 11.565068 130-139
GTCCA 2340855 1.3742672 28.418049 180-189
CAACT 1942965 1.366398 17.311012 130-139
GGCCT 2771615 1.3583575 69.5432 3
AACCC 2383335 1.3581017 19.728998 140-149
GAGAG 2706285 1.3499707 25.417852 220-229
CAACC 2364380 1.3473006 14.894137 140-149
AGCAG 2582340 1.3465723 12.397076 50-59
CGACA 2463050 1.3426256 16.304924 70-79
GTGCG 2819140 1.3216984 17.2697 240-247
AAGCT 1963180 1.3207082 16.268614 200-209
TGGAA 2051475 1.3202235 19.029495 120-129
CAATT 1520715 1.3198514 24.1983 180-189
ATACA 1629315 1.3130077 21.469563 35-39
GAACT 1946470 1.3094666 14.140347 190-199
ACAGA 2093940 1.3079647 20.134731 140-149
TGTGT 1751965 1.3077857 15.079385 240-247
TCCGT 2067860 1.3074732 14.030957 150-159
TTATT 1133295 1.307375 20.494154 240-247
ATCTA 1505575 1.3067111 20.031145 110-119
CACGT 2224380 1.3058872 16.308304 180-189
ACAGT 1935060 1.3017907 12.051811 120-129
AGAGC 2496330 1.301722 24.341572 220-229
ACATG 1930560 1.2987634 14.490545 190-199
CCATA 1846530 1.2985796 14.622482 160-169
CCTTT 1586215 1.2939104 48.438404 45-49
TCACT 1703685 1.2903765 13.18264 190-199
TGTCC 2035810 1.2872084 53.76656 15-19
GTGGC 2742610 1.2858187 13.98862 40-44
ATCAT 1481470 1.28579 15.820954 110-119
TTACA 1479525 1.2841018 9.3046665 120-129
TCAAG 1903775 1.2807441 22.520174 150-159
AATTC 1472175 1.2777227 24.12253 180-189
TGCGA 2269290 1.2744452 14.170509 240-247
CCTAC 2068930 1.2697198 17.443077 190-199
ATATT 1181185 1.2652028 25.85909 240-247
ATTAT 1176600 1.2602916 9.478988 50-59
CCAGT 2136455 1.2542683 18.62373 190-199
AACCA 1910285 1.2473702 16.847025 190-199
ACATA 1544200 1.2444166 20.535343 130-139
AGACC 2280755 1.2432554 98.02175 9
ACACA 1902195 1.2420878 7.8459516 130-139
CTTCT 1519080 1.2391471 23.863699 30-34
GATCT 1693085 1.2267059 9.551589 220-229
ACGTC 2088170 1.2259212 18.094555 180-189
CGGCT 2495750 1.2231572 21.24242 230-239
TCGGA 2159330 1.2126914 92.30492 5
AGATC 1795220 1.2077148 12.481262 210-219
ATCCG 2049225 1.2030573 23.272387 70-79
GATTG 1733430 1.2014412 23.089745 100-109
TGGAC 2127720 1.1949388 15.931389 80-89
AGTCG 2126715 1.1943744 15.779444 150-159
TGAGT 1722650 1.1939696 20.923136 90-99
CGCTG 2393420 1.1730057 12.554437 190-199
ACGCA 2146835 1.1702546 21.100595 140-149
TACCA 1663810 1.1700809 10.697902 45-49
GTCGA 2066090 1.160327 12.335216 150-159
GGGTG 2585785 1.1596919 14.653941 60-69
CTTCG 1830090 1.1571352 104.81202 3
ATTCC 1526790 1.1563956 22.618683 180-189
ATTAG 1390070 1.154113 24.828289 110-119
GAAGT 1789590 1.151688 13.559017 140-149
AAGGC 2206280 1.1504741 28.65828 25-29
GATCC 1955865 1.1482477 23.101988 70-79
AGGCC 2503940 1.1394366 14.093093 2
GACCC 2342880 1.1145042 42.6749 10-14
GTATA 1340270 1.1127664 10.265893 240-247
TCGAG 1978735 1.1112683 11.997613 150-159
GCAAG 2129040 1.1101971 17.036827 25-29
GCCCC 2673005 1.1096383 12.412685 80-89
AGCTC 1889390 1.1092216 12.0809765 210-219
GGACT 1974255 1.108752 12.783117 90-99
GAACC 2029505 1.1062973 10.313675 180-189
ACAAG 1770405 1.1058708 17.30526 80-89
AGGTC 1968275 1.1053939 17.921583 15-19
CGCAG 2426820 1.1043426 16.608835 140-149
TTGGG 1901885 1.1004364 15.005187 100-109
TTCGG 1817765 1.0994714 100.476395 4
GCTTC 1727175 1.0920638 27.667673 2
CACAT 1551245 1.0909193 13.849122 130-139
CCCCT 2035105 1.0899308 9.870793 70-79
CTTGA 1499485 1.0864352 22.241581 90-99
TTGAG 1557915 1.0797917 20.645874 90-99
GCACC 2261020 1.0755635 10.906216 130-139
CCGAT 1831735 1.0753735 23.212328 160-169
CTCCG 2081135 1.0662204 10.050536 140-149
ATGGG 1980270 1.0638739 19.987522 100-109
ATCAA 1311005 1.0564928 12.276199 110-119
TAGAC 1567820 1.054734 17.069815 170-179
TACAG 1562455 1.0511247 8.869909 120-129
GAAGC 2012830 1.0495988 12.701832 200-209
ATGAG 1626290 1.0465963 19.950006 60-69
GTAGA 1621065 1.0432338 16.544975 170-179
CAGCA 1912535 1.042536 12.456434 45-49
ATGGT 1502620 1.0414667 9.471208 35-39
ACACC 1825435 1.0401922 13.0987015 40-44
GGAGA 2080880 1.0380011 81.563065 7
CGTGA 1843340 1.0352297 20.817865 150-159
CTATA 1191725 1.034316 13.031153 50-59
GAGCC 2262265 1.0294605 18.035257 220-229
TGGCT 1696185 1.025934 30.620583 40-44
GACAG 1954945 1.0194144 15.626616 70-79
GCTCT 1602090 1.0129746 17.002659 210-219
ACCGC 2128965 1.0127453 11.052537 220-229
CGAGG 2319485 1.0096998 14.646107 220-229
GGGAG 2422455 1.0087672 14.084784 5
AAACT 1246470 1.0044863 16.903902 130-139
TGAGG 1869435 1.0043292 11.916179 220-229
AGCCG 2201110 1.0016315 19.0369 220-229
GTAGC 1783290 1.0015051 11.101544 50-59
TTATA 932355 0.9986735 13.236749 110-119
TAGTT 1115175 0.99717116 14.551487 50-59
ACTCA 1416005 0.9958111 12.091512 190-199
TTTCA 1063050 0.99367845 7.190206 100-109
ATGAC 1457455 0.9804871 10.802426 160-169
ATGAT 1173890 0.9746285 9.003363 120-129
AAGGT 1512100 0.97310954 20.809664 15-19
CCGTC 1886940 0.96672887 10.247034 140-149
AACTC 1372345 0.9651071 15.902649 190-199
CCGAG 2112600 0.9613543 17.963737 220-229
TTTAG 1072445 0.9589627 29.43253 45-49
ACCAA 1468365 0.9588071 16.546722 130-139
CGAGT 1695755 0.95234495 14.674308 160-169
CGTAT 1314270 0.95223975 7.613141 240-247
GGTAT 1370180 0.94967246 9.252965 110-119
GCCAA 1735760 0.94617486 14.398785 180-189
GCCAT 1602810 0.9409765 7.8523664 60-69
GATAC 1397520 0.9401665 23.432444 35-39
GGGAC 2156010 0.9385372 9.8097 90-99
GCAGT 1663830 0.93441576 5.981629 50-59
TTTAT 808920 0.93317425 10.87898 240-247
CAGAA 1493645 0.93299466 18.011604 140-149
CATAC 1326005 0.9325183 14.1012335 130-139
ACAGG 1787905 0.93231076 15.162254 70-79
CCAAC 1635915 0.9321975 13.838949 130-139
TATGG 1343610 0.9312568 12.233679 35-39
CGGAC 2039245 0.92797357 10.715657 220-229
AACTG 1373890 0.9242696 11.4974165 60-69
AGCCC 1940420 0.9230547 11.373051 80-89
AATCA 1141795 0.92013246 13.725452 110-119
TACAC 1306835 0.9190369 21.656061 35-39
TAACA 1139570 0.9183394 8.545793 120-129
GAGAT 1425880 0.917623 12.057603 210-219
AAGTT 1087940 0.90326804 17.510818 140-149
TCATA 1038055 0.9009435 8.746202 110-119
CCCAG 1890695 0.8994005 14.566026 80-89
TATTT 772355 0.8909927 10.897151 240-247
ACGCT 1517590 0.89094555 15.309308 190-199
TGGTT 1191975 0.88977116 9.1593895 50-59
CCCCA 1789150 0.88970065 16.762665 80-89
TCCTT 1085590 0.88553965 10.722599 45-49
GTTAT 984435 0.8802656 10.752281 110-119
CATTA 1011825 0.8781781 12.0523405 110-119
GTCTG 1440875 0.87151027 15.906876 3
AGAAG 1455765 0.86987644 15.698751 140-149
GACTG 1545660 0.8680508 12.427364 90-99
CGGAG 1993080 0.8676118 71.43035 6
AGTCT 1189535 0.8618644 15.156064 8
GATGA 1335270 0.85931104 6.545653 60-69
GCCTA 1452075 0.85248303 16.749418 190-199
GGTAG 1578970 0.8482807 11.909315 120-129
GATCA 1258105 0.84637654 9.362626 110-119
CATGG 1506880 0.8462718 8.715805 200-209
CTATC 1112045 0.8422665 8.38267 110-119
GGTTC 1392050 0.84197855 11.575283 160-169
GTTTC 1078190 0.8413408 5.089046 100-109
TCTTC 1031110 0.841099 47.197544 2
ATAGT 1012505 0.84063774 15.174378 120-129
ATCCA 1195110 0.8404658 9.891794 180-189
TTACC 1101695 0.8344273 9.856644 45-49
ACAAA 1104345 0.8263271 12.616942 130-139
GCTTG 1362305 0.82398736 17.104698 90-99
GTCTA 1136090 0.82314134 9.876146 30-34
TCATC 1082300 0.81973755 10.591604 110-119
TGGCA 1456335 0.81788546 5.83338 100-109
GGTGC 1738845 0.8152233 11.768543 60-69
ATATG 980450 0.8140239 5.6511235 50-59
ATATA 815990 0.81154394 10.409964 130-139
ACATC 1135770 0.7987348 5.367521 110-119
TTATG 890755 0.7964985 11.286136 50-59
CCTCC 1467975 0.7861959 10.502052 140-149
TGATG 1132610 0.78501254 11.90436 120-129
GTTCA 1062280 0.7696631 13.94371 160-169
TATAC 885875 0.7688642 8.749743 130-139
CTGAC 1293260 0.75924605 9.522388 220-229
CGCCG 1891730 0.75123423 10.92005 220-229
CCAGC 1573190 0.748364 6.184377 80-89
ATCTT 789950 0.7384001 12.607763 3
TCAAC 1048075 0.73706293 7.528824 110-119
CCCCC 1693080 0.73472464 13.423072 80-89
GGGAT 1366850 0.7343221 13.161802 100-109
ATAAA 792335 0.7316798 7.8041906 130-139
GGTTA 1051530 0.7288159 8.082229 50-59
CATCC 1182510 0.7257165 6.7232666 110-119
GACCG 1580725 0.71932065 9.242355 210-219
GCGGA 1647535 0.71719193 8.969843 220-229
TTTAC 767245 0.7171768 13.382884 45-49
AAATA 771890 0.7127999 12.552778 130-139
GATTA 857805 0.7121973 5.642605 160-169
ACGCC 1493770 0.710584 12.737846 180-189
TATTG 794260 0.71021426 6.467661 240-247
TTGGA 1022075 0.7084007 6.2668743 60-69
GGGCA 1626900 0.7082092 13.624823 150-159
AACGC 1297870 0.7074779 14.075354 180-189
ATGCC 1205015 0.7074392 8.029385 50-59
AATAC 876590 0.70641303 11.3695135 130-139
TGCCA 1199685 0.70431006 7.5632305 60-69
GACCA 1288905 0.702591 6.175841 170-179
CACAA 1067275 0.69690496 8.250582 130-139
TTTCT 691590 0.696235 9.41003 4
TCCGG 1415710 0.6938339 11.428209 70-79
GGATA 1071770 0.6897359 15.198562 35-39
CAACG 1264395 0.6892305 14.357725 180-189
ATTAA 692965 0.68918926 6.4219728 110-119
CAAAC 1047030 0.6836855 13.710437 130-139
GAAAT 883085 0.68076825 7.1479397 110-119
TATAG 819880 0.6807098 6.31836 120-129
AGGCA 1281490 0.6682385 6.601679 80-89
CAGTC 1137080 0.66755605 5.2920547 210-219
AATAA 721265 0.6660504 7.3429136 130-139
CACGC 1398260 0.66515005 12.454571 190-199
GCCGC 1669820 0.66311044 11.9399605 220-229
GCGAC 1439155 0.65489817 7.3392277 70-79
CCCGT 1277410 0.6544508 9.990053 140-149
CCGTA 1108845 0.6509798 6.373095 230-239
GACGA 1243755 0.6485614 9.676564 220-229
AGATG 1000080 0.6435999 5.59181 120-129
TACTT 678070 0.63382107 10.975675 1
TCCAC 1028700 0.63132197 10.497574 180-189
ACTTC 832880 0.63082606 29.310282 4
TCAAT 724785 0.62905174 8.3409605 110-119
CACCG 1296720 0.6168476 8.990231 45-49
CACGA 1130980 0.616505 8.906266 180-189
CCCTA 1001950 0.6149053 12.043873 110-119
AGTAA 795395 0.6131682 5.2228246 50-59
AAGCA 980280 0.61232483 5.891321 120-129
GGCCC 1533415 0.6089419 10.51317 70-79
CCGGC 1530285 0.607699 9.311153 70-79
GGTGA 1117440 0.6003299 6.446132 120-129
GAAGA 1001345 0.5983427 6.1287827 15-19
TAGCT 822220 0.5957303 12.183213 50-59
AATGG 923175 0.5941078 6.571293 120-129
ACGAC 1079635 0.58851653 9.0696745 220-229
CTTTG 753600 0.5880545 7.0978365 45-49
TGATT 657335 0.58777815 5.890753 50-59
GTTCC 922260 0.58312947 6.9481606 140-149
ACCCG 1209320 0.57527155 8.554537 140-149
TAATA 575980 0.5728417 8.047089 130-139
GGGGA 1375380 0.57274055 8.875343 4
CGGTT 937740 0.5671902 11.457349 160-169
ATTTC 606000 0.5664542 6.4359465 240-247
GTCCT 894960 0.56586814 6.4130816 45-49
TTAGC 778535 0.5640788 9.590949 45-49
ACCTA 792515 0.5573393 6.8073206 130-139
TTCAA 642090 0.5572795 5.367871 200-209
TCAGG 982705 0.55189234 5.3049393 150-159
AAGAT 712330 0.54913354 5.57098 15-19
CCTAT 724835 0.54899234 5.323908 110-119
CCACG 1146755 0.54550946 7.90046 180-189
CTGCG 1097305 0.5377848 11.790588 25-29
CGGCA 1181595 0.53769356 10.623532 70-79
CTGTT 688285 0.53708744 5.8886395 240-247
GGAGG 1288760 0.53666997 5.3374057 6
AAATC 659700 0.531629 7.692789 110-119
GCTAC 899530 0.52809536 7.8208284 50-59
TATGT 574525 0.5137308 6.4661345 140-149
ACATT 591115 0.51303756 5.9560223 110-119
ACCCC 1020075 0.5072584 8.896385 140-149
GGGTT 860570 0.4979284 5.987784 100-109
TGACG 879985 0.49420422 8.621987 220-229
GTAAC 730570 0.49148306 6.7814593 120-129
AGTAT 585845 0.48640096 5.0052133 110-119
TATCC 636530 0.48210984 5.488617 110-119
CGCGG 1266325 0.48105633 6.4412136 220-229
AACAT 593380 0.47818407 5.469203 110-119
TGGCC 974665 0.47767946 15.266622 2
GGGTA 874730 0.46993712 5.0545354 100-109
CCGGT 957805 0.46941644 8.943834 160-169
CAATC 664400 0.46724194 6.264626 110-119
TAAAT 469590 0.46703136 7.877724 130-139
ATGCT 640515 0.46407798 7.1321716 50-59
GGTTT 615010 0.4590853 5.4472456 100-109
TGCTA 627525 0.45466623 6.3654633 50-59
CATAA 560935 0.4520378 5.6727138 110-119
CCGCG 1128965 0.4483288 6.6990128 220-229
TTGGT 592325 0.44215164 5.3857236 120-129
ATTCT 471265 0.44051158 7.495865 2
TGCGC 884995 0.43373248 8.7623825 25-29
GCATG 754710 0.42384914 8.376024 60-69
GCCTT 665495 0.42078134 8.165469 1
GGTCA 743450 0.41752544 5.328855 160-169
ACGAG 793070 0.41354975 7.7837696 180-189
CACTT 545520 0.41317862 6.9917383 3
GCGCT 839865 0.4116145 7.9506884 25-29
ATGGC 732260 0.41124108 7.04944 1
CTAAC 569080 0.40020776 5.372471 110-119
CATGC 675705 0.39669237 7.0077357 60-69
CCTAA 556015 0.3910197 7.5258703 110-119
CCGGG 1021820 0.3881728 5.227361 80-89
AGCTT 529255 0.38346583 6.8462596 1
TTTGA 425680 0.38063607 5.774593 45-49
GAGGC 872245 0.3796988 6.028121 35-39
GGTAA 579995 0.37325487 7.1219935 120-129
TTCTT 369305 0.37178537 20.312344 1
CTCGG 721910 0.35380524 5.1649275 6
CGAGC 761675 0.34660587 6.664095 180-189
TGGGC 735720 0.34492785 9.63268 1
TGCTG 561475 0.339607 13.95683 1
GCTTT 394805 0.30807704 5.221107 45-49
CTCTT 376860 0.307413 9.145841 1
CGCTT 480320 0.30369827 5.990146 1
GCGAG 695865 0.30291843 5.3177834 240-247
ATTGT 327640 0.2929703 5.333262 240-247
GACTT 404135 0.29281154 6.465862 1
AACTT 337370 0.29280847 10.149686 3
TTCCT 343745 0.28040034 6.2735214 4
TCGGT 462960 0.28002042 6.1244097 7
GAGGT 517455 0.27799588 6.713324 7
CCCTT 415620 0.27470943 5.809183 3
TAACT 309435 0.26856327 7.161512 2
GTCTT 338845 0.2644099 11.662111 1
TTGGC 437090 0.26437297 7.773055 1
TGTAA 316785 0.26301244 7.7104607 25-29
AATCT 299775 0.2601792 7.3351893 2
CGGGG 693775 0.25211802 8.823103 3
CCTCG 468230 0.23988655 5.7388363 5
TGCTT 278895 0.21762931 11.290019 1
TCGCA 318890 0.18721369 7.0620294 7
TAGGC 275550 0.15475033 6.8413076 1
TTGCT 161460 0.1259916 5.7873197 4
Produced by FastQC (version 0.10.1)