FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_7_SL248353.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_7_SL248353.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6181044
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26587 0.4301376919497742 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20768 0.3359950196115737 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20302 0.3284558401460983 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20110 0.32534956877834875 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 19889 0.32177412100609537 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19250 0.311436061610304 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19111 0.309187250568027 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 17650 0.285550466879058 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17390 0.28134405773523047 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16675 0.2697764325897049 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16025 0.2592604097301362 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15557 0.2516888732712467 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15407 0.24926209876519242 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15348 0.2483075674594777 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15173 0.24547633053574766 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14892 0.2409301729610726 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14840 0.2400888911323071 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14612 0.23640019388310457 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14450 0.23377927741656587 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14446 0.2337145634297378 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14385 0.232727675130609 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14226 0.23015529415419145 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14005 0.22657984638193807 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 13906 0.22497817520794222 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13858 0.22420160736600483 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13537 0.2190083099230486 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12339 0.19962647086802812 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12321 0.1993352579273016 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12162 0.196762876950884 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 12106 0.1958568811352904 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12065 0.19519356277030223 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 11850 0.19171518597829104 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 11764 0.19032383526148658 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 11679 0.18894866304138913 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11508 0.1861821401044872 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11330 0.1833023676906361 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11322 0.18317293971697987 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11129 0.1800504898525233 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10844 0.1754396182910201 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 10711 0.17328787822898525 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 10418 0.16854757869382583 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 10391 0.16811075928273606 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 10255 0.16591048373058015 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 10084 0.1631439607936782 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10060 0.16275567687270953 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 9969 0.1612834336723699 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 9873 0.15973029798849514 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9765 0.15798302034413605 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 9432 0.15259558094069545 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 9337 0.15105862375352772 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 9293 0.15034676989841847 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9130 0.14770967493517276 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 8695 0.14067202886761523 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 8680 0.1404293514170098 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 8586 0.1389085727265491 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 8432 0.1364170842336667 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 8401 0.13591555083574877 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 8023 0.1298000790804919 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 7830 0.12667762921603534 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 7825 0.12659673673250021 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7767 0.12565838392349254 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 7637 0.1235551793515788 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 7614 0.12318307392731713 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7399 0.11970469713530595 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7385 0.11947819818140754 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 6868 0.11111391538387366 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6619 0.1070854697038235 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6334 0.1024745981423203 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6326 0.10234517016866407 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 12527880 7.656832 66.07896 35-39
TGGAT 9571440 7.2676105 79.17266 35-39
TATTA 6109320 7.225081 125.42172 240-247
TCACC 9702065 6.4102435 73.90688 160-169
TCTCC 8569665 6.065452 85.652985 20-24
CTGTG 8993990 5.8886123 85.69077 25-29
TCCCT 8172600 5.7844167 72.01264 15-19
AGTGG 9677090 5.688489 39.597652 100-109
CTGAG 9189035 5.6161857 49.06228 15-19
TGTAT 5600445 5.492868 59.3876 240-247
TTACT 5181540 5.283904 110.61911 240-247
ATTAC 5188065 4.9386973 103.24817 240-247
TGGGT 7744145 4.8765717 44.482716 100-109
CCTGA 7612615 4.837543 50.515675 15-19
CTCTG 6937455 4.722589 72.84242 35-39
TTCAC 5667120 4.651742 74.09539 40-44
GACAC 7739105 4.5908456 46.095234 230-239
CTCCA 6894295 4.5551243 40.25978 80-89
ACACG 7677330 4.5542006 48.698586 230-239
TGAGA 6233155 4.418077 92.81205 15-19
ATTCA 4534780 4.316813 110.071175 40-44
TCTGG 6572385 4.3031206 134.45523 35-39
TACTG 5425535 4.283279 78.056244 240-247
AAGGG 7794500 4.2771196 39.318638 90-99
CCCTG 7590795 4.15934 84.428566 10-14
AGCCT 6529350 4.1491666 50.50667 30-34
TGGAG 7046650 4.1422358 38.106464 90-99
GAAGG 7540030 4.137483 31.264692 90-99
CAGCC 8046725 4.1159234 54.341537 30-34
CCAGG 8325375 4.0957413 51.965454 80-89
ACCAT 5323885 4.079368 66.77895 160-169
TCAGT 5069085 4.0018735 99.294754 7
GAGAC 7003255 3.9956048 86.79181 8
CACCA 6428090 3.964636 56.79066 160-169
TCCAG 6207460 3.9446173 39.816807 80-89
GTATT 4012205 3.935136 68.4375 240-247
ACTAC 5133560 3.9335337 34.44817 130-139
TACTA 4079210 3.8831406 28.251253 130-139
GGGTC 7611235 3.8579218 123.45202 8
CACCT 5792105 3.8268971 43.83273 20-24
CTGGG 7469380 3.7860196 130.3644 3
ACTGT 4758790 3.7569063 67.21293 240-247
TGAAG 5255705 3.7252584 38.811424 150-159
GACTC 5820885 3.698963 50.8457 20-24
GATTC 4678955 3.6938787 57.75919 40-44
GGACA 6396670 3.6495261 28.383286 220-229
AGACT 4942365 3.6423328 68.246445 15-19
CAAGA 5205475 3.5810978 43.30621 180-189
ACTGG 5808075 3.549799 44.799267 60-69
GCTGG 6962515 3.529104 33.163387 2
CCTGT 5122275 3.4869273 60.41924 25-29
ACTCT 4178835 3.4301133 67.01245 20-24
GAGTG 5784950 3.4005704 39.19342 90-99
TCCTG 4924670 3.3524098 64.31475 20-24
TATCT 3230825 3.2946515 47.887127 200-209
CTCCT 4629940 3.2769868 93.265915 20-24
GGATT 4300855 3.265647 55.382893 40-44
AGACA 4689230 3.225948 36.93774 170-179
CTGCA 5069695 3.2216086 46.060593 25-29
AGCTG 5196615 3.1760848 35.496964 60-69
GGTCC 6010120 3.167385 139.96436 9
GTCTC 4615980 3.1422727 33.776203 30-34
CATCT 3824340 3.1391332 32.17036 170-179
CCATC 4681735 3.0932653 35.39365 45-49
GTCCC 5586265 3.060967 73.42077 10-14
CCTCA 4628570 3.058139 83.669945 5
GGAGT 5102075 2.9991555 36.40602 90-99
GGCTG 5801755 2.940747 34.039284 90-99
AAGAA 3636455 2.9012685 36.875492 180-189
AGTAG 4064270 2.880766 28.606516 50-59
AGGGA 5242725 2.87687 39.678288 80-89
GAGCT 4564340 2.7896488 28.564524 60-69
TGTGC 4256630 2.7869325 56.601234 25-29
GTGAA 3926395 2.7830393 40.464275 10-14
CAGGG 5835955 2.7613487 35.789307 80-89
ATCTG 3497115 2.760856 35.923946 200-209
GTGTA 3632170 2.7579129 36.023262 230-239
ATCTC 3336155 2.7384162 47.107517 170-179
GGAAG 4987465 2.7367997 29.882223 80-89
GACAA 3975130 2.7346842 24.135569 180-189
ACCTT 3262690 2.678114 72.112625 40-44
GAACA 3842040 2.643125 34.173893 210-219
ATGAA 3083225 2.635141 26.975813 210-219
CTATG 3317755 2.619257 26.409157 50-59
GGGAA 4767065 2.615858 33.564503 80-89
GAGTC 4262625 2.605246 27.670864 150-159
CACGG 5215505 2.5658135 43.933914 230-239
TGCAA 3454515 2.5458446 34.843365 200-209
AGGGC 5364070 2.538071 32.90395 150-159
CGCCA 4960460 2.5372896 39.008705 70-79
CAGTG 4130060 2.524224 76.800125 8
CCCTC 4419710 2.5179687 27.26015 20-24
AGAAC 3628255 2.496052 29.969995 190-199
CTACA 3256335 2.4951308 20.133835 190-199
AGGCT 4054230 2.477878 21.136913 25-29
ATCAG 3342705 2.463445 27.15687 45-49
GTGGA 4167955 2.450051 26.835089 100-109
ACGGC 4961375 2.440792 48.28783 230-239
TCTGT 2885430 2.4402447 24.519407 210-219
CCAAG 4111545 2.4389732 38.052982 180-189
CACTG 3814345 2.423879 19.702818 30-34
CTACT 2927735 2.4031727 22.620098 50-59
CTGTC 3495865 2.379768 48.66379 30-34
CTTCA 2883700 2.3670273 50.64584 45-49
GGGCT 4616620 2.3400354 42.250286 90-99
CCGCC 5295480 2.335606 33.469803 70-79
TATAT 1973405 2.333813 22.70246 240-247
GCAGC 4743735 2.333722 33.436886 30-34
TGAAC 3161985 2.3302612 36.950455 210-219
CTCTC 3282825 2.3235235 95.83788 20-24
ACAGC 3912325 2.320796 26.054646 210-219
GTATC 2887465 2.2795572 22.37738 200-209
TATGC 2865720 2.2623901 20.59982 50-59
CAAAT 2545700 2.2621703 34.673107 200-209
ACAAC 3141795 2.247259 20.478565 180-189
GGCCG 5503865 2.2455523 24.972284 230-239
CAGGC 4563365 2.2449877 33.329567 70-79
TGGTG 3563895 2.244223 34.272957 35-39
GCCTC 4094340 2.2434738 74.282455 4
TCCAA 2881795 2.2081435 40.94906 180-189
GTGGT 3501115 2.2046897 30.326488 120-129
TACAT 2315035 2.2037618 19.02191 190-199
GCCTG 4154710 2.189568 30.69322 210-219
AATGA 2555090 2.1837597 25.42241 210-219
AAATG 2549980 2.1793923 33.003773 200-209
CCTTC 3058835 2.1649878 54.92551 45-49
TATCA 2268070 2.1590538 17.242533 170-179
GCTCC 3937120 2.1573257 33.102623 80-89
CAGTA 2923025 2.154157 20.951946 45-49
GGGGG 5623090 2.122226 93.23336 5
GTAGT 2784070 2.113949 24.802624 120-129
GTGGG 4314085 2.103137 29.568504 100-109
TCTAT 2040140 2.080444 19.41712 110-119
CCTCT 2917730 2.0651164 47.66068 35-39
GCAGA 3612030 2.0607908 23.496756 140-149
TGCAG 3352730 2.0491328 42.5176 30-34
GCTAT 2581160 2.0377398 32.589676 50-59
CTCAC 3079485 2.0346441 55.15543 20-24
AACAG 2943825 2.0251997 32.762352 210-219
TGCAC 3181750 2.0218875 26.68247 25-29
AGAGT 2849460 2.019705 30.77298 150-159
GCTGT 3077905 2.015189 19.152195 230-239
ATGGA 2832125 2.007418 12.730719 100-109
CAGAG 3515900 2.0059454 31.764015 170-179
TGTGA 2640315 2.0047958 18.525986 210-219
AACTA 2217335 1.9703772 35.596714 130-139
TCTCT 2231430 1.9621196 33.343094 35-39
TGAGC 3210090 1.9619534 17.689184 200-209
AGCAC 3301560 1.9584891 20.223995 130-139
TTCAG 2465455 1.9463946 47.385048 45-49
GGTCT 2958500 1.9370114 36.960175 15-19
GGCTC 3672695 1.9355417 19.863277 40-44
ATATC 2031210 1.9335787 13.914266 170-179
TCCGC 3518725 1.9280683 41.399265 70-79
CATAT 2018750 1.9217178 13.769795 160-169
TCCAT 2319345 1.9037879 27.15196 45-49
CCGTG 3611735 1.9034156 20.027803 230-239
GCACA 3192770 1.8939545 18.248362 130-139
CTGTA 2396695 1.8921108 23.69686 190-199
TGGTA 2490030 1.890684 30.399158 120-129
ACCCT 2859150 1.8890667 43.63249 10-14
TGGGA 3213405 1.8889375 17.318573 100-109
GGATC 3086675 1.8865248 18.529465 70-79
GATGG 3206660 1.8849726 21.571648 100-109
TGGGG 3863885 1.8836623 121.2903 4
CAGAC 3169265 1.8800119 25.493828 140-149
GTCAC 2958330 1.8799123 38.36295 160-169
CCTGC 3421140 1.8745973 50.746456 20-24
ACCAG 3153960 1.8709326 14.09066 190-199
CACAG 3151470 1.8694556 16.370275 140-149
GCCAG 3797200 1.8680657 34.624092 70-79
ACTAT 1956180 1.8621553 17.000105 130-139
GGTGG 3796945 1.8510289 18.22901 40-44
CCAGA 3100945 1.8394841 33.305088 170-179
AGGAC 3219225 1.8366817 20.942165 220-229
CAGTT 2323605 1.8344088 24.558603 190-199
AGAGA 2764040 1.8288612 27.786442 170-179
TGTCT 2161040 1.8276188 50.195934 30-34
CTATT 1791860 1.8272592 18.117868 110-119
TAGTA 1979875 1.8126976 17.493176 120-129
GCTGA 2963970 1.8115292 26.67473 200-209
CAAGG 3169545 1.8083377 17.640724 80-89
TCAGC 2826895 1.7963899 12.361412 45-49
TTCCA 2186920 1.7950894 21.382982 180-189
GAGGA 3270370 1.7945684 27.06873 220-229
AGTCA 2407215 1.7740248 29.16715 160-169
TTCTG 2094740 1.771548 44.03472 30-34
ACCTG 2776345 1.7642673 30.833424 25-29
AGTTC 2232155 1.7622118 21.591192 190-199
GCCGA 3578935 1.7606883 18.765982 220-229
ATACT 1831625 1.7435869 25.978098 130-139
TGACC 2737855 1.7398083 14.072504 210-219
CCTGG 3294740 1.7363565 34.275185 2
TAGTG 2277720 1.7294767 25.885021 120-129
TACAA 1944315 1.7277652 17.013649 130-139
GGATG 2939195 1.7277486 23.335253 100-109
CATGA 2326160 1.7142904 22.6424 60-69
GCAAA 2483750 1.7086917 31.408857 200-209
CTGAA 2317515 1.7079194 23.038124 200-209
CTCAG 2661735 1.6914368 80.13388 6
ATGCA 2262145 1.6671138 16.0585 60-69
GTGCA 2724610 1.6652364 49.638924 25-29
ATTGG 2187665 1.6610978 20.151363 100-109
AACAC 2314175 1.6552798 21.360033 190-199
GGAGC 3489680 1.6511818 13.329575 200-209
AGGGG 3572720 1.6258816 32.83766 90-99
GGGGC 4123295 1.6180072 28.504885 90-99
TCTGA 2042645 1.6126002 21.899187 220-229
AGCTA 2183380 1.609067 24.675514 50-59
GCCGT 3053055 1.6089861 29.185644 230-239
CGATT 2023050 1.5971305 35.190014 160-169
GGGCC 3894760 1.5890448 29.562008 2
TCTGC 2319805 1.5791793 32.651695 200-209
GGCTT 2388105 1.5635579 22.343138 25-29
TCTCA 1902125 1.5613211 25.383268 100-109
AGTGA 2194695 1.5556058 88.82163 9
CGTCC 2822545 1.5465999 18.736984 180-189
GTTCT 1818020 1.5375224 22.695524 190-199
CTCCC 2695150 1.5354633 13.531266 190-199
CAGCT 2409770 1.5313221 12.1873455 50-59
GCACT 2409345 1.5310519 25.6328 25-29
AGTTA 1660055 1.5198828 16.072424 50-59
CCATG 2383215 1.5144473 16.053679 160-169
GAGCA 2637760 1.5049353 8.018326 200-209
TTCTC 1711330 1.5047903 21.265106 190-199
CGGCC 3545645 1.5040771 31.307507 230-239
CATCA 1945840 1.4909785 31.674377 45-49
ACAAT 1674010 1.4875656 21.567062 180-189
GTCCG 2794395 1.4726702 33.72004 70-79
TTAGT 1485635 1.457098 24.843668 110-119
CTGGT 2221570 1.4545228 31.829533 35-39
GTTAC 1836200 1.4496187 15.141688 50-59
GTGAC 2340450 1.430444 14.694942 210-219
AAAAA 1480095 1.423877 6.545892 90-99
TACGC 2221980 1.4119883 24.069267 140-149
ACTCC 2133760 1.409795 16.331413 140-149
CTCAA 1831895 1.4036692 18.974634 150-159
CTTTA 1376200 1.4033875 40.14478 45-49
GGCAG 2953810 1.3976288 14.065012 150-159
GGCCT 2637400 1.3899324 71.2405 3
GTCCA 2181735 1.386414 28.526321 180-189
CGTGT 2108930 1.3807744 23.802353 230-239
GGGGT 2825415 1.3774033 115.823814 7
CAACC 2226180 1.3730352 15.509325 140-149
ATACA 1544315 1.3723154 23.301367 35-39
AACCC 2224095 1.371749 20.036201 140-149
CGACA 2312385 1.3717092 16.945248 70-79
AGCAG 2398830 1.3686174 12.654489 50-59
CAACT 1778550 1.3627944 16.712431 130-139
GAACT 1837970 1.3545132 14.761095 190-199
AAGAG 2044370 1.3526827 18.678486 150-159
TTATT 1061785 1.3451649 21.462381 240-247
CCATA 1753000 1.3432169 14.139582 160-169
ATCTA 1409320 1.3415803 20.19017 110-119
CTACG 2109725 1.3406543 16.233109 130-139
AAGCT 1816805 1.3389153 16.132084 200-209
ACAGA 1938925 1.3338803 20.748503 140-149
GTGCG 2627815 1.3319658 17.200766 240-247
ACATG 1802115 1.3280894 14.980339 190-199
GAGAG 2418740 1.3272488 25.02899 220-229
ACAGT 1797575 1.3247437 12.213095 120-129
TAGCA 1794235 1.3222821 11.115421 130-139
CACGT 2080305 1.3219589 16.844631 180-189
TGTCC 1926815 1.3116561 54.389114 15-19
TCAAG 1779735 1.3115962 23.111866 150-159
TGGAA 1847670 1.3096335 18.667711 120-129
CCTAC 1981510 1.3092021 17.66469 190-199
TCACT 1591175 1.3060842 13.643946 190-199
ACACA 1819335 1.3013313 7.8837676 130-139
TGCGA 2127960 1.3005738 14.272605 240-247
ATTAT 1091490 1.2908316 9.559393 50-59
TGTGT 1585215 1.2894115 14.798493 240-247
CCAGT 2013710 1.2796402 18.623352 190-199
GTGGC 2520870 1.2777584 14.245602 40-44
AACCA 1772620 1.2679169 17.676067 190-199
TTACA 1326725 1.2629552 9.393435 120-129
CTTCT 1434980 1.2617928 23.850891 30-34
AGACC 2123715 1.2597901 99.95643 9
CCTTT 1424655 1.252714 46.051613 45-49
CAATT 1315380 1.2521557 22.380648 180-189
AGAGC 2193875 1.2516834 23.771292 220-229
TCCGT 1837055 1.2505529 12.987886 150-159
GATTG 1640620 1.2457256 23.646173 100-109
TGGAC 2034165 1.2432477 16.455622 80-89
ACGTC 1941155 1.2335341 18.701347 180-189
ATCCG 1938855 1.2320726 23.883978 70-79
TGAGT 1618100 1.2286261 21.772953 90-99
AGTCG 2003825 1.2247045 15.607046 150-159
ACATA 1377220 1.2238308 20.03035 130-139
AATTC 1281285 1.2196994 22.37465 180-189
ATATT 1031095 1.2194067 24.352926 240-247
ATCAT 1277100 1.2157155 15.297071 110-119
CGGCT 2303895 1.2141724 21.054882 230-239
TACCA 1557465 1.1933904 10.432135 45-49
GTCGA 1941035 1.1863283 12.19205 150-159
GATCT 1500745 1.1847881 9.602074 220-229
TCGGA 1937660 1.1842656 93.10457 5
AAGGC 2067840 1.1797758 29.913696 25-29
GATCC 1851370 1.176479 23.735733 70-79
GGGTG 2411735 1.1757324 15.139153 60-69
GAAGT 1651490 1.1705807 14.284558 140-149
CTTCG 1703225 1.1594499 104.447815 3
AGGCC 2354985 1.1585555 14.688973 2
ATTAG 1252145 1.146416 24.616695 110-119
TTGGG 1808860 1.1390587 14.89168 100-109
AGGTC 1861765 1.1378801 18.796324 10-14
ACGCA 1917690 1.1375759 20.331314 140-149
GGACT 1857810 1.1354626 12.550141 90-99
ACAAG 1645050 1.1317095 18.020346 80-89
TCGAG 1851525 1.1316212 11.792026 150-159
ACACC 1826930 1.1267908 13.498308 40-44
GCAAG 1971950 1.1250672 17.521332 25-29
AGCTC 1766755 1.1227092 12.46935 210-219
GAACC 1891185 1.1218531 10.395257 190-199
CTTGA 1421015 1.121844 22.908094 90-99
GACCC 2192895 1.121672 42.8654 10-14
AGATC 1520790 1.1207637 12.976811 210-219
TTGAG 1473070 1.1185048 21.430603 90-99
GCTTC 1642950 1.1184185 27.489208 2
TTCGG 1683505 1.1022369 100.45613 4
GCCCC 2489925 1.0981977 12.424199 80-89
CGCTG 2081710 1.0970792 11.488951 190-199
ATGGG 1863090 1.0951811 20.507925 100-109
ATCAA 1230465 1.0934209 12.292877 110-119
ATTCC 1329255 1.0910923 20.841787 180-189
CCGAT 1708785 1.0858713 23.441893 160-169
CAGCA 1818175 1.0785434 13.104344 45-49
GCACC 2094285 1.0712328 11.172998 130-139
CGCAG 2172245 1.0686549 15.9284115 140-149
CCCCT 1875550 1.0685263 9.744674 70-79
TAGAC 1446730 1.0661843 16.779566 170-179
GGGAG 2329730 1.0602188 14.663978 5
CACAT 1383575 1.0601491 13.15461 130-139
GGAGA 1931355 1.0598032 83.30157 7
GACAG 1849500 1.0552052 16.319769 70-79
GAAGC 1840775 1.0502273 12.619643 200-209
GTATA 1146835 1.0499982 9.520151 240-247
TACAG 1413545 1.0417283 8.754089 120-129
CTATA 1093480 1.0409213 13.399552 50-59
TGGCT 1577585 1.0328882 31.136652 40-44
TGAGG 1755215 1.0317689 12.227726 220-229
ATGAG 1449860 1.0276648 19.58369 60-69
TTTCA 1006730 1.0266184 7.7832727 100-109
ATGGT 1348005 1.0235425 9.37296 35-39
GCTCT 1497800 1.0196092 16.965315 210-219
ATGAC 1376380 1.0143391 11.363329 160-169
ACCGC 1980850 1.0132105 11.359328 220-229
AAGGT 1426500 1.0111072 21.363325 15-19
CTCCG 1837180 1.0066739 9.621158 140-149
GAGCC 2045550 1.0063263 17.391775 220-229
GTAGA 1419265 1.0059788 16.719116 170-179
GCCAA 1689100 1.0019759 15.18767 180-189
AAACT 1124430 0.9991955 16.903357 130-139
CCGTC 1822400 0.9985753 10.565593 140-149
TAGTT 1014295 0.9948118 14.433809 50-59
ACTCA 1296075 0.9931032 12.535164 190-199
AACTC 1291450 0.98955923 16.534298 190-199
CGAGG 2090850 0.98930943 14.363327 220-229
CGTGA 1616950 0.98825294 19.577473 150-159
GTAGC 1606985 0.9821624 10.992056 50-59
ACAGG 1720785 0.9817688 15.871642 70-79
TTATA 827095 0.97814965 12.520202 110-119
AGCCG 1985510 0.976789 18.444483 220-229
GATAC 1320735 0.97333086 25.1979 35-39
CGAGT 1592325 0.9732026 15.360955 160-169
TACAC 1258710 0.9644726 22.870369 35-39
ATGAT 1053345 0.9644024 8.418973 120-129
ACCAA 1343500 0.9609767 16.32528 130-139
AACTG 1300680 0.958551 12.477161 60-69
TTTAT 755275 0.9568504 11.382764 240-247
AAGTT 1040965 0.9530678 18.489946 140-149
CGGAC 1934330 0.9516106 10.607443 220-229
CAGAA 1383035 0.95145667 18.577091 140-149
GCAGT 1555010 0.95039624 6.76335 50-59
GGGAC 2008055 0.95013404 9.627856 90-99
TAACA 1068760 0.9497258 9.0976 120-129
CCGAG 1898710 0.93408704 17.322762 220-229
GAGAT 1309390 0.9280993 12.500758 210-219
CATAC 1209905 0.92707634 13.4789 130-139
GGTAT 1219675 0.92610145 8.903439 110-119
AGCCC 1810025 0.925833 11.614319 80-89
CCAAC 1499480 0.9248303 13.305531 130-139
TTTAG 941335 0.9232533 28.559511 45-49
TCATA 964555 0.91819316 8.626026 110-119
AATCA 1030655 0.9158648 13.391038 110-119
TGGTT 1124665 0.9148009 9.359191 50-59
TATTT 717865 0.9094559 11.221924 240-247
GCCAT 1420245 0.90251446 7.3090076 60-69
GTTAT 919640 0.901975 10.012247 110-119
CCCAG 1762435 0.90149075 14.494592 80-89
TATGG 1177060 0.8937437 12.12391 35-39
AGAAG 1348770 0.89243037 16.347044 140-149
CCCCA 1667580 0.8868583 16.553911 80-89
AGTCT 1121345 0.88526446 15.728173 8
TCCTT 1001165 0.8803348 10.763574 1
GATGA 1241975 0.88031524 6.6961465 60-69
GTTTC 1037930 0.8777905 5.5205603 100-109
GACTG 1430875 0.87452704 12.150711 90-99
CGGAG 1838360 0.8698409 72.02932 6
CGTAT 1095830 0.86512125 6.8451996 240-247
ATAGT 942370 0.8627979 15.733831 120-129
CATGG 1409900 0.86170745 8.788476 200-209
GCCTA 1351265 0.85868025 16.95842 190-199
GTCTG 1305380 0.8546681 14.128489 3
ATCCA 1113530 0.85323006 9.813607 180-189
TTACC 1033255 0.8481267 9.596332 45-49
GTCTA 1073950 0.84784776 9.81508 30-34
TTATG 862900 0.8463249 12.445812 50-59
GATCA 1146800 0.84514743 9.286103 110-119
TCTTC 959160 0.84339935 46.250145 2
CTATC 1021765 0.83869535 8.310379 110-119
GGTGC 1654430 0.83858424 12.103825 60-69
GCTTG 1274925 0.83472836 17.42513 90-99
CATTA 869635 0.82783556 12.532938 110-119
TCATC 1000935 0.82159746 10.493522 110-119
ACATC 1058275 0.8108915 5.717081 110-119
GGTAG 1373350 0.8072971 11.51218 120-129
GGTTC 1228860 0.80456847 10.696009 160-169
CCTCC 1410765 0.8037319 10.052488 140-149
ACGCT 1263330 0.8028007 14.089288 190-199
ACAAA 967050 0.80219144 12.66017 130-139
ATATA 725380 0.80080444 10.97737 130-139
TGGCA 1297700 0.79313266 5.3876295 100-109
TATAC 826325 0.78660715 9.200771 130-139
ATATG 854815 0.7826359 5.659384 50-59
TGATG 1024350 0.7777907 11.712094 120-129
CTGAC 1217315 0.77355987 9.544615 220-229
TCAAC 999970 0.766216 7.556884 110-119
GGGAT 1294670 0.7610465 13.740044 100-109
CCAGC 1485125 0.75964576 6.686288 80-89
TATGA 825420 0.75572294 5.4101677 50-59
GGTTA 979320 0.74359936 8.2205 50-59
ATCTT 728760 0.743157 12.642046 3
GCGGA 1569435 0.74259603 8.926895 220-229
ACGCC 1431565 0.7322497 13.244781 180-189
CATCC 1107425 0.73168594 6.666739 110-119
GTTCA 926640 0.7315514 12.979177 160-169
GACCA 1231995 0.7308207 6.3129773 170-179
CCCCC 1593645 0.7308114 13.223826 80-89
AACGC 1231290 0.7304026 14.77993 180-189
GGGCA 1543605 0.7303743 14.05099 150-159
TTGGA 961765 0.7302698 6.3912187 60-69
GGATA 1026730 0.72774905 16.25047 35-39
GATTA 794050 0.7270017 5.6590447 50-59
GACCG 1474940 0.7256096 8.926886 210-219
TATTG 738605 0.7244174 6.8157105 240-247
CGCCG 1696015 0.7194565 10.975817 220-229
TTTCT 656630 0.71730816 8.794684 4
CAACG 1208750 0.71703184 15.08504 180-189
TTTAC 703070 0.71695954 13.087973 45-49
ATAAA 679660 0.7004282 7.2012744 110-119
CACAA 978830 0.7001361 8.322437 130-139
GAAAT 817445 0.69864607 7.171334 110-119
TCCGG 1315840 0.69345903 11.34712 70-79
TATAG 753920 0.6902603 6.6669745 120-129
ATGCC 1076235 0.6839086 7.7977853 50-59
CAGTC 1075870 0.68367666 5.6005626 210-219
TGCCA 1075150 0.68321913 6.9981422 60-69
CCCGT 1237385 0.67801917 10.342493 140-149
CAAAC 947550 0.67776227 13.589471 130-139
GCCGC 1595360 0.6767582 12.12121 220-229
AATAC 760685 0.675963 10.811024 130-139
GCGAC 1371220 0.67458373 7.46601 70-79
AAATA 633265 0.6526155 11.948647 130-139
ACTTC 794715 0.6523259 30.06778 4
GACGA 1142815 0.65201634 9.819548 220-229
AGGCA 1137190 0.64880705 6.0994873 80-89
TCCAC 978245 0.6463355 10.386638 180-189
AGATG 908800 0.64416 5.838207 120-129
TACTT 629860 0.6423032 11.107545 1
TCAAT 666425 0.634393 8.158266 110-119
CCCTA 950600 0.62807024 12.288839 110-119
AAGCA 912345 0.6276462 5.757171 120-129
CACCG 1221345 0.6247215 8.991711 45-49
CACGC 1208050 0.61792105 11.383629 190-199
ATTAA 552795 0.61027414 6.624749 110-119
CACGA 1028635 0.6101874 8.858962 180-189
ACCTA 796030 0.6099492 7.7884483 130-139
GGCCC 1436705 0.6094561 10.382611 70-79
AATAA 590895 0.6089508 6.671892 130-139
CCGGC 1421415 0.60297006 9.157769 70-79
GTTCC 882850 0.6009895 7.3273125 140-149
AATGG 844530 0.5986052 6.597744 120-129
GGTGA 1018310 0.5985938 6.6923523 9
CTTTG 706825 0.5977708 7.343878 45-49
ACGAC 1004815 0.5960575 9.148932 220-229
ACCCG 1163565 0.59516686 9.047482 140-149
TAGCT 749935 0.59204865 12.050306 50-59
TGATT 597620 0.5861405 6.1152816 50-59
GGGGA 1281225 0.58306277 9.2487335 4
ATTTC 571040 0.58232117 6.6199694 240-247
CCGTA 902765 0.57367474 5.956605 230-239
GAAGA 864455 0.5719773 6.296156 15-19
AGTAA 667980 0.5709027 5.1189632 50-59
TTCAA 597325 0.56861424 5.5168114 200-209
GGAGG 1244315 0.5662657 5.5571012 6
TCAGG 908195 0.5550737 5.4078703 150-159
CCACG 1069445 0.5470242 7.802596 180-189
CGGCA 1102355 0.54231316 10.568822 70-79
CTGCG 1023880 0.5395936 11.9034815 25-29
AAGAT 631140 0.5394167 5.7825704 15-19
GTCCT 792200 0.53928053 6.290135 45-49
CTGTT 637090 0.53879505 5.7433996 240-247
TAATA 487920 0.53865355 7.2935243 130-139
TTAGC 678380 0.5355584 9.092183 45-49
ACATT 561565 0.53457314 6.6142635 110-119
CCTAT 650265 0.53375703 5.1677966 110-119
CGGTT 813170 0.5324047 10.600035 160-169
GCTAC 836755 0.5317277 7.821565 50-59
TATGT 536885 0.52657217 6.7143383 140-149
AAATC 588465 0.5229241 7.6235833 110-119
AGTAT 558060 0.5109384 5.617224 110-119
GGGTT 806890 0.50810736 6.2699766 100-109
ACCCC 947570 0.50394 8.518703 140-149
CGCGG 1222980 0.4989704 6.5964203 220-229
GTAAC 675750 0.49800175 7.145814 120-129
TGACG 810010 0.49506465 8.695676 220-229
GGTTT 606725 0.49350923 5.892941 100-109
TATCC 597880 0.4907578 5.3682995 110-119
CATAA 548215 0.48715705 5.93327 110-119
TGGCC 915460 0.48245525 15.160076 2
AACAT 540495 0.48029685 5.853819 110-119
CAATC 604855 0.46346346 6.0547047 110-119
CCGCG 1083180 0.45948943 6.822804 220-229
ATGCT 572220 0.4517486 6.953026 50-59
GCCTT 650025 0.44249663 9.213017 1
TGCTA 560170 0.44223556 6.2127953 50-59
ATTCT 432155 0.44069242 7.0899224 2
CCGGT 833905 0.43947512 8.192869 160-169
TGCGC 829465 0.43713516 8.8141575 25-29
GGTCA 707700 0.4325345 5.462577 160-169
TTGGT 531050 0.4319553 5.2385607 120-129
TAAAT 385375 0.425446 7.1551414 130-139
CTAAC 549510 0.42105597 5.8779445 110-119
CACTT 505460 0.41489673 7.0837193 3
CCTAA 540260 0.41396827 8.091991 110-119
GCATG 670585 0.40985042 8.042267 60-69
ACGAG 708615 0.40428996 7.6418037 180-189
TTTGA 411145 0.4032475 6.256516 45-49
GCGCT 762510 0.4018493 7.9639463 25-29
TACCT 486320 0.39918604 5.065121 2
CCGGG 975245 0.39789563 5.327663 80-89
ATGGC 650425 0.39752892 7.225345 1
AGCTT 501595 0.39599252 6.9675407 1
GAGGC 818135 0.3871099 5.981865 35-39
GGTAA 544105 0.38566312 7.5235105 120-129
CATGC 605180 0.3845701 6.691043 60-69
CGGTG 757895 0.38415575 5.7863045 8
TTCTT 337390 0.3685677 19.676678 1
TGGGC 695595 0.3525776 9.389262 1
TGCTG 530305 0.34720522 14.032404 1
ATGTG 452180 0.34334105 5.0740943 140-149
CGAGC 681765 0.33540028 6.4181995 180-189
CTCTT 368400 0.32393795 9.024533 1
CTCGG 613925 0.32354376 6.310423 6
TCACA 413400 0.31676316 6.3620996 5
GCTTT 368540 0.31167892 5.0290575 45-49
GCGAG 649365 0.30725443 5.4862165 240-247
CGGGA 649065 0.30711251 5.1109543 80-89
GACTT 381395 0.30109861 6.8416567 1
CGCTT 435540 0.29648858 5.8892965 1
ATTGT 301520 0.2957282 5.591446 240-247
AACTT 309460 0.29458565 10.811256 3
GAGGT 498760 0.29318637 6.9951477 7
TTCCT 329290 0.28954813 6.057086 4
CCCTT 396165 0.2803984 5.7103534 3
GTCTT 322770 0.27297065 11.942314 1
TAACT 280790 0.26729366 7.0875235 2
AATCT 280220 0.26675108 7.386168 2
TTGGC 400070 0.26193684 7.608204 1
TGTAA 283985 0.2600058 7.7964396 25-29
CGGGG 656465 0.25760105 9.387731 3
CCTCG 453870 0.2486959 6.9035916 5
TCGGT 368775 0.2414471 7.5283823 7
CTGGC 423605 0.22324346 5.0779138 3
TGCTT 258735 0.21881546 11.26596 1
TCGCA 272470 0.17314488 6.0635586 7
TAGGC 255170 0.15595564 7.142243 1
TTGCT 151270 0.12793095 5.5655484 4
Produced by FastQC (version 0.10.1)