FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_16_SL248345.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_16_SL248345.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6871328
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 7945260 8.568064 60.276764 110-119
CACAG 10554100 7.4178553 42.16312 110-119
ATACA 7237265 7.4007983 37.05483 110-119
TCCCT 12462680 7.148338 109.02613 30-34
CAGTA 9012130 6.67967 40.034668 60-69
AGTAA 6858735 6.274299 47.50671 110-119
GGAGA 10906285 6.134344 92.0516 35-39
CCCTG 12545945 6.104409 71.056725 30-34
GTAAT 7512700 5.8721085 39.654255 110-119
GGTGA 12170855 5.8491025 103.08848 45-49
AATAC 5272175 5.391305 39.160137 110-119
GTAGT 9154095 5.186052 25.33233 80-89
AGACG 8108930 5.098445 100.28346 40-44
ACAGT 6867245 5.0899105 35.89519 110-119
GTGAC 9297555 4.994819 84.062515 45-49
ACTCC 7130695 4.786837 125.2633 30-34
GAGAC 7492605 4.7109346 140.7009 40-44
CCCCA 7393535 4.706505 100.88496 60-69
ACCAG 6627660 4.6581917 94.435616 45-49
CTCAG 7666735 4.604096 34.604443 140-149
CACTC 6557825 4.4022694 99.661385 25-29
GCACA 6233565 4.3812056 34.56532 110-119
GGCCC 10579380 4.366637 60.07802 60-69
CGTGT 9469070 4.346454 23.267223 120-129
GTGTC 9468740 4.346303 23.78988 120-129
TAGTA 5517260 4.3124237 31.849504 80-89
TGACC 7078335 4.250745 141.9259 45-49
CTCCC 7786315 4.235017 71.4173 30-34
AGTAG 6190310 4.10447 28.831846 70-79
ATGCA 5505785 4.080814 97.74636 25-29
GACCA 5767370 4.053544 106.28346 45-49
CCCAG 6959020 3.9628859 86.53114 60-69
TGAGG 7920040 3.8062341 68.939995 35-39
CAGGG 8280930 3.7737818 37.3652 50-59
CCACT 5602580 3.7610133 27.43708 240-247
ATGGT 6494670 3.6794128 25.85251 190-199
AGGAG 6535525 3.6759684 92.54058 35-39
TACAC 4433170 3.6730332 26.949287 110-119
ACGGT 6801300 3.6537848 94.33629 40-44
GACGG 7999660 3.6456015 106.56349 40-44
TGCAC 6027980 3.6199763 117.91647 25-29
CTGAG 6727685 3.6142368 106.326126 35-39
GGGTT 8717565 3.579646 44.245068 50-59
AGATA 3887860 3.5565736 19.049438 150-159
TGTCC 6887515 3.5340545 20.200087 120-129
GAGGA 6168055 3.4692814 122.1701 35-39
CCTGA 5746920 3.4511917 85.352776 30-34
GCCCC 7412125 3.4198928 73.178764 60-69
CCCTT 5914615 3.3925018 31.196238 20-24
CCAGG 6644210 3.3847291 42.283913 50-59
TGGTG 8220485 3.3755329 18.146667 190-199
TCTTG 6221995 3.3667495 18.595339 170-179
GCACT 5588405 3.3559988 90.450714 25-29
GGATG 6947400 3.3388002 70.7937 20-24
CTTCA 4647965 3.290411 16.858185 200-209
CTCTC 5683635 3.2600164 13.862878 100-109
CCGTG 7434860 3.2361612 23.687548 120-129
CCTGG 7403290 3.2224197 30.171753 50-59
CTTGG 6988135 3.2076657 17.657509 20-24
TCAGG 5964295 3.2041302 20.708378 140-149
CAGAT 4307215 3.1924503 17.639805 150-159
AGGCT 5871355 3.154201 21.489332 30-34
TGTCT 5826665 3.1528347 20.116365 180-189
TCGCA 5228880 3.1400936 24.221313 100-109
GGTTG 7594835 3.1186256 77.002205 15-19
TTCCC 5429985 3.114528 51.463634 50-59
CGGAT 5755135 3.0917654 80.42152 20-24
CGCAC 5375615 3.0611994 24.513027 100-109
TCTCA 4295530 3.0409133 21.129005 130-139
GGTTC 6610985 3.0345478 49.89891 50-59
GATGC 5573590 2.9942358 99.82373 25-29
ACAGA 3451305 2.9938781 14.788503 140-149
AGGGT 6209605 2.9842284 51.901688 50-59
TGGCC 6781955 2.9519722 44.814617 60-69
AAAAA 1983430 2.929799 15.770531 130-139
ACCAC 3721640 2.92398 19.703743 230-239
GTTGG 7084870 2.9092214 54.713703 15-19
TCAAA 2790410 2.8534622 58.903976 70-79
AGTCA 3837235 2.8441072 33.66134 70-79
GACTC 4723320 2.83649 19.517803 190-199
CCAGT 4704230 2.8250258 37.855927 60-69
TCTCT 4645820 2.8101304 15.020047 180-189
CGGTG 7103425 2.7659369 67.11905 40-44
TTCTT 4334585 2.7649164 21.044302 170-179
GCCGT 6335100 2.7574701 22.577322 120-129
GCCCT 5645355 2.746828 17.173359 20-24
GTTCC 5327160 2.7334204 56.454884 50-59
CGGCC 6551480 2.704122 12.8764715 120-129
GATGG 5524505 2.6549814 17.670116 15-19
TCACA 3155645 2.61456 12.085267 140-149
AGATG 3924155 2.601901 16.58367 180-189
GCGGA 5666185 2.5821912 89.26455 20-24
TCCAT 3609090 2.5549653 16.544323 70-79
AGGGA 4541270 2.5542808 9.13541 150-159
GGCGG 7591785 2.507639 43.776314 20-24
TGTTC 4579465 2.4779692 12.812364 140-149
ACAGC 3488840 2.4521 11.21332 120-129
TCCTC 4262085 2.4446445 16.795944 130-139
TTGGG 5944375 2.440906 81.92646 15-19
GGCTG 6246955 2.4324439 11.806725 140-149
CTGGC 5556815 2.4187071 36.75083 60-69
ACTCT 3382430 2.3945067 13.630924 200-209
GTTCT 4420925 2.3921824 17.737661 160-169
TGGAG 4951370 2.3795428 14.454982 25-29
TACCA 2863280 2.3723254 16.29216 230-239
GAGAT 3565265 2.3639402 16.267881 180-189
CAGCT 3925605 2.357439 12.502802 150-159
AATAA 1846390 2.3303504 9.074842 110-119
CACGG 4552440 2.3191285 9.673541 120-129
CTGGA 4238850 2.2771885 14.427731 180-189
TCATT 3011695 2.248375 16.539478 150-159
CATTT 3009505 2.2467399 17.559965 150-159
TCAGC 3736550 2.2439063 14.583756 140-149
TGTAG 3950930 2.238313 8.116122 210-219
CTACT 3157330 2.2351527 7.6756506 180-189
ACTAC 2689715 2.228521 12.2966 230-239
CACTA 2671505 2.2134333 15.231447 240-247
TTCAT 2947750 2.2006369 15.782618 140-149
TTCAC 3098795 2.1937146 18.353714 200-209
CAGAG 3481830 2.1891816 9.741884 140-149
GTGAA 3281245 2.1756215 17.87076 190-199
TGGGG 6234255 2.1715815 53.721756 15-19
TAGTC 3395370 2.1502624 27.948458 70-79
GATAC 2889655 2.1417737 14.324269 150-159
TTGTC 3940135 2.1320248 13.031571 170-179
TCTGC 4146265 2.127491 12.052937 210-219
TTGGA 3728095 2.1120703 16.302246 170-179
ATTTG 3144270 2.099881 15.47606 150-159
CTCGC 4308020 2.0961287 16.600708 100-109
GTCAA 2809095 2.0820637 42.401245 70-79
TCTCG 4054850 2.0805852 16.976978 100-109
ATATA 1912285 2.062183 10.38828 110-119
ACACA 2108220 2.0443234 13.219268 110-119
AGCTC 3398045 2.0406241 12.471186 140-149
GAACT 2751905 2.039675 11.110796 160-169
GAATC 2744245 2.0339975 14.250838 190-199
GTCTC 3962825 2.0333662 11.874216 180-189
GCTGT 4426305 2.0317447 10.240851 140-149
TACAG 2736330 2.0281312 13.76411 160-169
CTCTG 3950090 2.0268319 14.466396 180-189
ATCTC 2853665 2.020181 8.794636 100-109
GATAT 2584005 2.019721 8.243574 180-189
AACCA 2071635 2.0088475 15.290266 70-79
GTCCT 3906295 2.0043602 14.210541 130-139
GTTCA 3156540 1.9990131 14.874463 140-149
TGAAT 2546000 1.9900154 13.877781 190-199
CAGGC 3885065 1.9791507 15.278858 140-149
AATAT 1834710 1.9785271 12.830442 110-119
ACACG 2787615 1.9592503 13.352757 120-129
AATCG 2633460 1.9518855 13.060873 190-199
TTGCA 3073835 1.9466366 14.208269 150-159
ATGTA 2487600 1.9443682 6.992172 150-159
ACGGC 3797405 1.9344944 14.489269 120-129
CCTTC 3370810 1.9334275 14.084122 200-209
GGGGC 5852275 1.9330622 48.976814 15-19
TACTA 2196940 1.9195487 9.47817 240-247
ATATG 2442410 1.9090469 8.43545 180-189
GAGTC 3551155 1.9077464 12.204369 210-219
TGGAA 2872835 1.9048262 9.358716 170-179
CAGCC 3338790 1.9013084 10.361473 120-129
TTTGC 3501655 1.8947611 12.248632 150-159
GGGCG 5715055 1.887737 61.95175 20-24
TCGGC 4304405 1.8735723 7.8932395 130-139
CATAG 2520735 1.8683351 8.179738 210-219
AGTAT 2323225 1.815889 11.82421 220-229
CTGTT 3291220 1.7808939 12.725032 140-149
GTTGT 3676955 1.7798629 7.1007714 210-219
AGAAC 2042405 1.77171 11.7337055 160-169
GACGT 3278750 1.7614055 10.020877 170-179
CGTAG 3237005 1.7389795 9.428095 210-219
GTCAC 2893890 1.7378646 8.871263 140-149
CTCCA 2572760 1.7270947 7.824754 150-159
GCAGA 2734255 1.719148 14.360368 150-159
ATTGT 2572685 1.7181512 7.662278 170-179
AGTCT 2694945 1.7066884 12.974175 210-219
TGCAG 3163405 1.6994401 13.511607 150-159
TGGTT 3509080 1.6986016 18.448782 10-14
CACCA 2154135 1.6924386 13.162153 230-239
GGTCA 3140440 1.6871028 7.755511 130-139
ATAGT 2149190 1.679859 7.4133797 220-229
CCAGA 2376965 1.6706285 24.051317 60-69
AACTG 2252120 1.6692413 10.980461 160-169
TGACT 2629670 1.6653504 11.776365 190-199
TTCAG 2622645 1.6609015 9.656853 150-159
GTCTG 3610960 1.6574885 9.029309 210-219
CAAAA 1384870 1.6574343 20.508627 70-79
TCCCA 2445465 1.6416414 10.320008 240-247
GAGAA 2113990 1.6404794 10.814456 160-169
CAAAG 1866630 1.6192317 30.25497 70-79
TCAGA 2169000 1.6076338 9.884768 130-139
ACTGA 2163015 1.6031978 8.977514 180-189
CTGGT 3485700 1.5999918 7.2470765 160-169
TTGTA 2392285 1.5976723 9.023046 210-219
TCTGG 3476340 1.5956956 11.932037 180-189
GTGCT 3471555 1.5934992 8.244977 220-229
TGTGC 3469345 1.5924847 8.092903 220-229
TAGTT 2376830 1.5873507 10.076576 220-229
GCTCC 3262325 1.5873309 6.2437806 150-159
AATAG 1725000 1.578012 9.465665 240-247
TGCTC 3075005 1.5778167 5.1230316 150-159
CCTTG 3046600 1.5632418 20.070734 20-24
ATAGA 1708185 1.5626298 10.034074 240-247
GAACC 2219565 1.5600014 12.859088 70-79
GGACG 3422255 1.5595886 7.5332146 170-179
GGCCG 4205080 1.5526669 13.373215 120-129
CTGTG 3371630 1.547632 5.1706166 210-219
TTGGT 3189210 1.5437655 22.04814 25-29
GCTCA 2564855 1.5402696 11.213568 140-149
CCCAC 2381145 1.5157663 10.272829 240-247
GTCCC 3110345 1.5133829 18.393532 50-59
CCACC 2357535 1.5007367 9.598992 230-239
ACCAT 1793670 1.486117 10.9816 45-49
GCTCT 2891545 1.4836814 10.036852 130-139
CCTCG 2953810 1.4372185 9.543427 130-139
CCATC 2138725 1.4357266 10.384155 240-247
CGACT 2389550 1.4349939 8.715011 190-199
TGATA 1830555 1.4308062 7.7672653 180-189
AATTG 1826090 1.4273162 9.480809 170-179
TATAC 1630965 1.425035 6.63981 110-119
TGGAC 2652365 1.4248998 10.65668 170-179
CTCTT 2346050 1.419062 8.463916 200-209
CACTG 2360105 1.4173112 7.415543 230-239
GGGAG 3446690 1.4051317 6.5972605 150-159
TCGAC 2338550 1.4043667 9.433258 190-199
GAGGG 3444270 1.4041449 8.348375 200-209
CTGCT 2676705 1.3734448 6.0374336 90-99
CTTGA 2165935 1.3716704 8.687358 200-209
CATGT 2140935 1.3558382 6.6936073 70-79
GCGTA 2512820 1.3499336 9.96987 210-219
GCTGA 2512410 1.3497133 18.489174 30-34
CAGAC 1917660 1.3478103 13.712771 60-69
CCTCA 1990500 1.3362232 7.3064265 130-139
ACGGA 2124485 1.3357583 9.204471 200-209
CTGAT 2107395 1.3345975 7.500029 180-189
GAATT 1689770 1.320765 10.485113 170-179
AGATC 1780460 1.319653 6.971415 130-139
CTCGA 2193450 1.3172301 8.45896 190-199
ACTTC 1857205 1.314762 6.556587 220-229
TCCAC 1935510 1.2993083 9.54692 240-247
ACTAT 1482480 1.2952982 6.2606044 230-239
GGAAT 1953170 1.2950445 8.798012 170-179
ACGTG 2404425 1.2917016 9.494865 170-179
TATGG 2273155 1.287806 6.1865263 190-199
ATCGG 2388145 1.2829559 7.7657886 190-199
AAAGT 1399470 1.2802205 22.448633 70-79
ATAAC 1246805 1.2749778 7.987786 80-89
AGCCG 2488190 1.2675471 9.177783 120-129
GGAGT 2633210 1.265475 7.0346446 210-219
GTGTT 2612250 1.264483 7.5667267 160-169
AAGTA 1373735 1.2566785 16.222107 70-79
GTCCA 2084145 1.2515893 15.444666 70-79
GGCTC 2843940 1.2378778 8.2721195 130-139
ACTCG 2059195 1.236606 8.669183 190-199
CCATG 2048025 1.2298981 6.7861605 70-79
GTGGA 2558070 1.2293642 18.75438 25-29
ACTGG 2272720 1.2209474 7.4073787 160-169
TTGAG 2152815 1.2196302 8.30485 200-209
GATCT 1905810 1.2069352 5.426223 130-139
TTCCA 1703460 1.205922 7.8361015 230-239
ATAAA 954240 1.2043575 5.251468 1
AGCTT 1895655 1.2005041 6.4850082 150-159
AGAGC 1896815 1.192612 7.4105678 140-149
GGAGG 2903515 1.1836922 14.441432 30-34
ACCGT 1963875 1.1793636 16.117321 45-49
GCATA 1585835 1.1753998 7.932899 210-219
TATCA 1331070 1.1630055 6.7068768 70-79
GTGGT 2818565 1.157372 9.454679 50-59
TCACG 1921730 1.1540543 9.448721 200-209
TTGGC 2512085 1.1530871 7.6462398 60-69
ATACC 1372160 1.1368816 6.6986427 70-79
ACTGT 1793840 1.1360255 5.6116643 230-239
TGGTC 2474270 1.1357296 10.74356 50-59
AAAAG 1053795 1.1282376 13.618695 70-79
TCTAC 1592850 1.1276184 7.5202255 180-189
ATATC 1288220 1.125566 7.1684384 70-79
TGCCC 2301825 1.1199859 6.028499 60-69
AGGTT 1965565 1.1135478 23.376722 10-14
GTCTA 1757900 1.1132649 7.630546 180-189
GCTTC 2164045 1.1103938 5.8320274 150-159
TCTGT 2025860 1.0962019 6.4067426 210-219
TACTG 1722580 1.0908971 6.7062182 180-189
CAATA 1066400 1.0904964 6.662481 100-109
AGTTC 1720660 1.089681 5.284045 160-169
GAAGA 1401235 1.0873737 17.366945 35-39
GAGCT 2022865 1.0867207 6.540451 140-149
ATCCA 1310120 1.0854793 14.957477 240-247
CCATA 1306295 1.0823101 9.274091 70-79
CGGCT 2476120 1.0777774 7.8065968 130-139
CTACC 1600720 1.0745637 8.839492 230-239
AGGGG 2635685 1.0745045 5.0573664 60-69
CTCGG 2460680 1.0710568 8.385262 130-139
TATGT 1600095 1.0686132 7.355491 220-229
CTGAA 1440915 1.0679868 13.914908 35-39
GGGGT 3065735 1.0678891 8.025205 10-14
CTGCG 2429705 1.0575744 6.725173 210-219
AAAAT 833530 1.052008 5.2727513 8
CTGCC 2127990 1.035404 5.6588783 200-209
GTCCG 2377230 1.0347337 5.1797094 130-139
GCGGT 2655545 1.034018 5.8850436 130-139
TAGAT 1308865 1.0230407 7.7616034 240-247
CTTCT 1671470 1.0110269 5.3357916 210-219
ATACG 1361820 1.0093628 5.1607695 120-129
CATGG 1875485 1.0075454 5.1569376 190-199
TATAA 929595 1.002463 6.4768634 240-247
AGAGA 1288720 1.0000608 12.14582 35-39
CAGCG 1962545 0.9997702 6.456659 160-169
TGCTA 1566255 0.99189764 5.0023413 220-229
TGCGT 2155995 0.9896361 8.024647 210-219
AACTT 1126460 0.9842303 5.6535416 200-209
CGAAC 1399175 0.98339766 13.603652 70-79
ATGGG 2013550 0.96767724 27.253773 15-19
ACCCA 1227425 0.9643506 12.613346 240-247
CGGAG 2111645 0.9623179 6.6003065 210-219
AGCTG 1789180 0.96118057 5.212027 90-99
TGTAA 1206760 0.9432329 6.847668 230-239
TCCCC 1733610 0.94291943 5.0120263 80-89
ACAAT 918165 0.93891186 7.6828837 100-109
AAGAG 1205955 0.93583435 12.827364 35-39
ACTAA 906925 0.9274179 12.262724 240-247
TAGGC 1701860 0.9142707 5.5980077 160-169
TTCTG 1672545 0.9050216 5.414177 210-219
CCATT 1276070 0.9033618 5.523669 50-59
TCGAA 1216875 0.9019314 17.004427 70-79
GAGTT 1591475 0.9016153 5.0136747 160-169
CCCAA 1138585 0.8945518 9.688271 240-247
CATCA 1069590 0.8861919 5.6187096 230-239
TGCAT 1398495 0.8856565 6.048735 210-219
TGAAC 1191550 0.88316095 6.461338 190-199
CGTGG 2252205 0.8769652 6.5196733 50-59
AGCGT 1611460 0.86570626 6.6198506 160-169
CCAGC 1517785 0.8643183 5.8037257 80-89
CTTCC 1496890 0.8585854 5.530624 230-239
CGGTC 1971125 0.85796887 5.713762 130-139
GTATG 1509200 0.8550042 5.199931 220-229
TGAAG 1289200 0.8548009 9.1253 35-39
CCAAT 1023540 0.84803796 10.542743 240-247
ACGTC 1396380 0.8385665 10.008128 70-79
ATCAA 808065 0.82632405 12.257098 70-79
GGTGG 2349395 0.81836605 18.987068 25-29
TAACC 977825 0.81016153 6.532174 80-89
CGTCC 1656430 0.80595976 8.867451 70-79
AAAGA 745295 0.79794437 6.0753946 8
GAGGC 1726665 0.786875 12.212668 30-34
AACCG 1098230 0.77188116 5.6114583 190-199
TGAGA 1155190 0.76594585 5.3779488 240-247
AAAGG 985605 0.76484025 8.816397 10-14
GGGCC 2043900 0.75468135 14.361558 15-19
ATGAT 951195 0.7434771 7.1574473 240-247
AGCCA 1055785 0.742049 5.8014827 80-89
CTAAT 848540 0.74140114 9.432715 240-247
GTCGA 1365400 0.73351824 12.4252615 70-79
GACCG 1424950 0.72590566 15.011898 45-49
AATCC 873680 0.7238738 9.232111 240-247
GTAGC 1322065 0.7102379 5.514069 80-89
AAGGT 1070120 0.7095404 10.045801 10-14
GGTCC 1611355 0.70137227 11.002271 50-59
TGGGC 1797740 0.70000535 15.601522 15-19
GGAAG 1210470 0.68084043 5.97491 30-34
CATAC 821545 0.6806782 7.8888144 70-79
AAAGC 760090 0.6593497 9.244836 70-79
TATGA 832965 0.6510656 5.7648835 240-247
TAGGT 1145065 0.64871144 5.5076985 10-14
CATTG 1023730 0.64832056 5.02151 50-59
GAAAA 597940 0.6401799 5.7961726 8
CAATC 761310 0.6307714 9.014802 240-247
ATAGG 932650 0.6183913 5.3997145 10-14
GAGGT 1265085 0.6079779 6.6187015 10-14
GGGTC 1553375 0.6048543 6.0987606 70-79
AGAAG 777760 0.60355026 5.500029 9
AATGG 886325 0.58767563 6.782648 10-14
TAAAA 463130 0.5845218 5.9061055 8
AAGAA 541405 0.5796512 5.512981 8
AAATG 628905 0.5753157 5.1785445 9
CGATG 1045015 0.5614015 12.298854 15-19
CATCC 822405 0.5520807 6.752297 240-247
AAGGG 943815 0.53085774 6.569305 10-14
GAAGG 941085 0.52932227 5.5670757 10-14
AAGCA 591335 0.51296103 6.185576 70-79
GGTCG 1305845 0.5084709 6.188674 70-79
TGATC 797640 0.50513947 5.4307785 240-247
ATAAG 529415 0.48430333 6.045213 9
GATCC 793195 0.47633648 5.4239464 240-247
CGGTT 983135 0.45127466 8.029384 10-14
TAAGG 670615 0.4446496 6.129264 10-14
ACGAT 578355 0.428669 5.458016 10-14
TAAAG 437940 0.40062293 5.245211 9
TGGGT 961080 0.39464304 5.012302 10-14
AAGCT 531695 0.39408523 7.5748806 30-34
Produced by FastQC (version 0.10.1)