FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_8_SL248354.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_8_SL248354.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10599471
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 46039 0.434351865295919 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 37108 0.35009294331764296 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 36770 0.3469041049312744 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 35989 0.33953581268348204 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 32883 0.3102324634880363 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 31785 0.29987345594888654 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 31657 0.2986658485126286 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 31301 0.2953071903305363 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30636 0.28903329232185265 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29719 0.2803819171730363 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 28376 0.26771147352542407 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 28347 0.26743787496564686 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27578 0.2601827959150037 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27521 0.2596450332285451 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27459 0.25906009837660765 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27093 0.25560709586355773 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27064 0.2553334973037805 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26842 0.25323905315652073 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25770 0.24312534087786078 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25591 0.24143657735371887 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25565 0.24119128209322901 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25391 0.23954969073456592 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24884 0.23476643315501314 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24212 0.2284264941146591 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23773 0.22428477798561833 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23412 0.2208789476380472 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22607 0.21328422899595648 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21886 0.20648200273391004 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21826 0.2059159367481641 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21567 0.203472418576361 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21552 0.20333090207992457 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21338 0.20131193339743086 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 20795 0.19618903622643052 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20158 0.1901793023444283 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20013 0.18881130954554243 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19903 0.18777352190500826 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 19736 0.18619797157801554 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 19465 0.18364124020906328 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19449 0.18349028927953104 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19229 0.18141471399846276 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19207 0.18120715647035593 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18328 0.17291428977917858 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18232 0.17200858420198517 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 18139 0.17113118192407903 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17670 0.16670643280216532 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17420 0.1643478245282241 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17278 0.1630081350286255 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 17202 0.16229111811334734 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17149 0.16179109315927181 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 16809 0.15858338590671175 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16526 0.1559134413406103 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16084 0.15174342191228224 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 15951 0.15048864231054548 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15533 0.14654504927651577 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15035 0.14184670159482488 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 14607 0.1378087642298375 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14334 0.1352331639946937 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 14242 0.13436519614988332 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14125 0.13326136747767883 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13920 0.13132730869304704 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13778 0.12998761919344842 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12579 0.11867573391162634 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12483 0.11777002833443292 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12368 0.11668506852841995 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 11713 0.11050551485069396 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11337 0.10695816800668637 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11096 0.10468446963060704 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10945 0.10325987023314653 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 10850 0.10236359908904888 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 21572270 7.6798134 68.09618 35-39
TATTA 10710110 7.367285 128.75166 240-247
TGGAT 16350015 7.19944 80.73699 35-39
TCACC 16694515 6.476355 75.559044 160-169
TCTCC 14972095 6.198118 88.90332 20-24
CTGTG 15650820 5.9458284 88.809425 25-29
TCCCT 13991265 5.7920766 72.49894 15-19
AGTGG 16656535 5.680526 40.006935 100-109
CTGAG 15955430 5.6801963 51.288265 15-19
TGTAT 9826190 5.5865335 60.545456 240-247
TTACT 8928365 5.298833 112.26412 240-247
ATTAC 8988530 4.9989233 105.920105 240-247
TGGGT 13613445 4.954415 46.15101 100-109
CCTGA 13324165 4.951603 53.01969 15-19
TTCAC 9973810 4.78568 76.50911 40-44
CTCTG 12028460 4.7701974 74.42934 35-39
CTCCA 12102660 4.6950226 42.429817 80-89
ACACG 13348625 4.6485963 50.21995 230-239
GACAC 13341950 4.6462727 47.76158 230-239
TGAGA 11030445 4.5514855 96.90119 15-19
ATTCA 8058895 4.481912 114.92488 40-44
AAGGG 13565900 4.3354278 39.724277 90-99
TCTGG 11402780 4.331976 136.46846 35-39
TACTG 9322980 4.2853503 78.9044 240-247
AGCCT 11427040 4.2465825 52.75256 30-34
CAGCC 13990150 4.203417 57.040905 30-34
GAAGG 13096265 4.1853404 31.70877 150-159
CCCTG 13016985 4.1736016 86.12738 10-14
CCAGG 14500295 4.1735535 53.798714 80-89
ACCAT 9188000 4.131265 68.0582 160-169
GAGAC 12350665 4.1202607 85.47206 8
TGGAG 12055015 4.1112285 38.858162 90-99
TCCAG 10979550 4.080284 41.801365 80-89
TCAGT 8687295 3.9931543 97.1156 7
GGGTC 13566320 3.991725 129.7476 8
CACCA 10913200 3.9672399 58.104862 160-169
GTATT 6975145 3.9656146 69.60104 240-247
ACTAC 8758595 3.9381888 34.8233 130-139
TACTA 7002670 3.8944979 29.313093 130-139
GACTC 10376350 3.8561192 53.33594 20-24
CTGGG 13097735 3.8538494 136.71973 3
GATTC 8324475 3.8263824 60.22544 40-44
AGACT 8834970 3.805539 71.50298 15-19
CACCT 9766780 3.7888567 44.253117 40-44
ACTGT 8208765 3.7731957 67.995285 240-247
TGAAG 9071575 3.743198 40.513073 150-159
GGACA 11029840 3.6796253 28.818012 220-229
CAAGA 8999070 3.6323545 44.536938 180-189
GCTGG 12106875 3.562301 34.268417 2
ACTCT 7410805 3.5558867 70.196 20-24
ACTGG 9988085 3.5557978 44.569115 60-69
CCTGT 8887445 3.5245461 62.92307 25-29
TCCTG 8573330 3.399976 64.944016 20-24
TATCT 5682785 3.372636 49.949806 200-209
GAGTG 9862025 3.3633337 38.957905 90-99
CTCCT 8038080 3.3275886 95.7486 20-24
AGACA 8188430 3.3051507 38.01643 170-179
GGATT 7467050 3.2879834 57.5222 40-44
GGTCC 10676155 3.2791739 145.77394 9
CTGCA 8725490 3.242617 45.219345 25-29
CATCT 6716460 3.2227228 33.70585 170-179
AGCTG 8904865 3.1701672 36.081394 60-69
GTCTC 7929745 3.1447456 33.528683 30-34
CCATC 8054200 3.1244907 34.88304 45-49
GTCCC 9622905 3.0853665 77.002556 10-14
GGAGT 8807705 3.0037696 37.220158 90-99
CCTCA 7710805 2.9912765 82.52677 5
GGCTG 10127695 2.9799514 34.869263 90-99
AAGAA 6309725 2.9519317 37.48955 180-189
AGTAG 6982100 2.881019 29.03041 50-59
AGGGA 8968565 2.866199 40.699863 80-89
TGTGC 7445530 2.8285959 58.760868 25-29
GTGAA 6814605 2.811906 39.546726 150-159
ATCTC 5842375 2.8033156 49.290394 170-179
GACAA 6936490 2.7998219 25.389088 180-189
ATCTG 6089760 2.7991858 37.354477 200-209
GAGCT 7818550 2.783435 29.293272 60-69
GAACA 6868840 2.7725158 35.90978 210-219
CAGGG 9975865 2.7506077 36.66667 80-89
GGAAG 8590555 2.7453935 30.514912 80-89
ATGAA 5432200 2.712015 28.208977 210-219
GTGTA 6140695 2.7039466 35.812565 230-239
ACCTT 5634550 2.7035959 71.91036 40-44
CTATG 5774675 2.6543555 27.243412 50-59
CGCCA 8673710 2.6060636 40.595684 70-79
GGGAA 8139660 2.6012952 34.18701 80-89
CACGG 9027800 2.5984302 44.917034 230-239
TGCAA 5990500 2.5803235 36.38301 200-209
AGAAC 6340125 2.5591068 30.980392 190-199
GAGTC 7165585 2.550977 27.162727 150-159
AGGGC 9176795 2.5302835 33.28704 150-159
AGGCT 7023830 2.5005116 20.436642 80-89
CAGTG 6994120 2.4899344 75.16165 8
CCAAG 7137365 2.4855547 38.940353 180-189
ACGGC 8605700 2.476939 49.442608 230-239
CCCTC 7381190 2.4704607 26.713491 20-24
CTACA 5441470 2.4466865 19.69657 190-199
CACTG 6565805 2.4400222 19.652428 60-69
TATAT 3538485 2.4340582 24.444073 240-247
ATCAG 5646440 2.4321246 26.861153 45-49
TCTGT 4911745 2.4092832 23.975988 210-219
CTCTC 5797295 2.399953 100.43894 20-24
TGAAC 5569160 2.398837 38.617584 210-219
GTGGA 6994730 2.3854747 26.181995 100-109
GCAGC 8256505 2.3764317 34.944305 30-34
ACAGC 6806540 2.3703458 27.610023 210-219
CCGCC 9138365 2.3688815 34.79131 70-79
CAAAT 4528860 2.3602402 36.395535 200-209
CTACT 4891930 2.3472683 22.6253 50-59
CTTCA 4889380 2.3460448 51.005646 45-49
GGGCT 7954825 2.340611 42.87084 90-99
CTGTC 5866875 2.326661 47.56236 30-34
GTATC 5010365 2.303037 23.305761 200-209
TATGC 4980895 2.289491 20.754417 50-59
GGCCG 9621710 2.2888908 25.032755 230-239
CAGGC 7948165 2.2876837 33.7909 70-79
AAATG 4552805 2.2729788 34.55374 200-209
GCCTC 7075380 2.2685604 72.10925 4
AATGA 4534290 2.2637353 26.6344 210-219
TCCAA 5021240 2.2577357 42.18947 180-189
ACAAC 5357575 2.257407 21.19367 180-189
GCCTG 7261470 2.230356 31.36909 210-219
TGGTG 6110910 2.2239764 33.300606 35-39
GCTCC 6929920 2.221922 34.78253 80-89
GGGGG 10094700 2.20376 97.81265 5
GTGGT 6019750 2.1908002 31.069275 120-129
TACAT 3907395 2.173077 18.451876 190-199
CAGTA 5022915 2.16355 21.570139 45-49
CCTTC 5162455 2.1371431 55.823597 45-49
GTAGT 4819545 2.1222014 25.270924 120-129
TGCAG 5957835 2.121013 44.360134 30-34
GTGGG 7521130 2.1199746 30.63067 100-109
CCTCT 5101190 2.1117806 49.717373 35-39
GCAGA 6259880 2.0883358 24.69447 140-149
AACAG 5171625 2.0874574 34.476974 210-219
TATCA 3746545 2.0836213 16.77913 170-179
GCTGT 5461040 2.0746777 19.664612 230-239
GCTAT 4486750 2.0623548 32.899647 50-59
TCTAT 3465695 2.0568311 19.491724 110-119
CAGAG 6128020 2.0443468 33.487724 170-179
TGCAC 5436150 2.0202134 26.291391 25-29
CTCAC 5185855 2.0117648 53.951458 20-24
GGCTC 6490730 1.9936231 19.72487 40-44
ATGGA 4829195 1.9926676 12.373337 100-109
AACTA 3798415 1.979565 35.583946 130-139
TGTGA 4492900 1.978369 18.241993 210-219
TCCGC 6160800 1.9753209 42.847515 70-79
CTGTA 4276445 1.9656874 24.84735 190-199
AGCAC 5614460 1.9552097 20.44422 130-139
TGAGC 5489605 1.9543214 17.242416 200-209
TTCAG 4244985 1.9512266 47.73265 45-49
TCTCT 3796010 1.943705 32.998592 35-39
GGTCT 5107575 1.9403948 35.493374 15-19
AGAGT 4701320 1.9399024 29.938137 150-159
CCGTG 6299725 1.9349562 19.78531 230-239
ATATC 3468720 1.9291103 13.450527 170-179
CAGAC 5530425 1.9259449 27.014847 140-149
CATAT 3460550 1.9245666 13.673916 160-169
CCAGA 5519860 1.9222658 35.1882 170-179
TGGTA 4347275 1.9142457 30.6793 120-129
GCCAG 6641185 1.9115018 35.927795 70-79
TGGGG 6760600 1.9056046 126.99986 4
AGAGA 4924920 1.9043151 29.246601 170-179
AGGAC 5679470 1.8947076 21.39032 220-229
ACTAT 3401580 1.8917707 17.193111 130-139
GCACA 5390090 1.877074 18.519909 130-139
GATGG 5477495 1.8680387 20.930182 100-109
TCCAT 3879900 1.8616717 26.732954 45-49
CTATT 3135425 1.8608214 18.94272 110-119
GGATC 5190225 1.8477409 17.959755 70-79
ACCAG 5302125 1.8464408 13.879603 190-199
GTCAC 4964410 1.8449026 37.652737 160-169
ACCCT 4737670 1.8378987 42.58174 10-14
CAGTT 3996690 1.8370966 24.27743 190-199
GGTGG 6509695 1.8348823 17.725254 40-44
TGGGA 5379635 1.8346646 16.607712 100-109
GCCGA 6364015 1.8317252 19.762863 220-229
GAGGA 5725750 1.8298512 27.762098 220-229
CCTGC 5694235 1.8257275 49.15832 20-24
CACAG 5240860 1.8251055 15.987841 140-149
TTCCA 3780405 1.8139313 22.738972 180-189
TAGTA 3402880 1.8129399 17.571932 120-129
ATACT 3240280 1.8020647 27.223927 130-139
TGTCT 3654185 1.7924315 48.90003 30-34
TCAGC 4798445 1.7832258 11.8219795 45-49
GCTGA 5002305 1.7808404 26.21998 200-209
GCAAA 4381450 1.768514 32.96398 200-209
CAAGG 5290860 1.7650644 17.291462 80-89
TAGTG 3969240 1.7477845 26.033339 120-129
TTCTG 3558655 1.7455724 42.279224 30-34
CATGA 4043150 1.74153 23.762384 60-69
AGTCA 4039370 1.7399018 28.539675 160-169
ACCTG 4660655 1.7320195 30.135548 25-29
CCTGG 5612870 1.7239891 35.774048 2
AGTTC 3748075 1.7228196 21.394466 190-199
GTGCA 4819295 1.7156883 51.74667 25-29
TACAA 3288785 1.7139685 17.08611 130-139
TGACC 4602070 1.7102478 14.010071 210-219
CTGAA 3965845 1.7082319 22.763588 200-209
AACAC 4054145 1.7082086 23.184217 190-199
CTCAG 4575515 1.7003795 78.772835 6
ATGCA 3926620 1.6913363 16.644743 60-69
GGATG 4954755 1.689764 22.527117 100-109
AGCTA 3831250 1.650257 25.454832 50-59
AGGGG 6236825 1.6473681 33.58419 90-99
TCTGC 4117610 1.6329448 33.97146 200-209
ATTGG 3701890 1.6300619 19.749146 100-109
GGAGC 5892795 1.6247984 12.99779 200-209
GGGGC 7114555 1.6213232 29.161089 90-99
CGATT 3515235 1.6157936 35.637787 160-169
TCTCA 3365560 1.6148785 26.705769 100-109
GGGCC 6744115 1.6043453 28.52959 2
GCCGT 5199370 1.5969831 29.1633 230-239
TCTGA 3463855 1.5921767 21.381401 220-229
ACAAT 2975860 1.5508858 23.169352 180-189
CAGCT 4159165 1.5456529 12.516154 50-59
GCACT 4135730 1.5369438 25.281593 25-29
AGTGA 3723105 1.5362624 86.75075 9
GTCCG 4990300 1.5327672 35.26595 70-79
AGTTA 2871165 1.5296601 16.04589 50-59
CTCCC 4562500 1.5270542 13.557726 190-199
CCATG 4097375 1.5226902 15.698798 160-169
GTTCT 3101280 1.5212234 22.5339 190-199
CGTCC 4717570 1.512582 18.509855 180-189
GGCTT 3957790 1.5035852 21.110195 25-29
CGGCC 6054770 1.5035625 31.533567 230-239
TTAGT 2624985 1.4923962 25.472397 110-119
TTCTC 2906410 1.4881952 21.194645 190-199
GAGCA 4423065 1.4755628 7.662657 200-209
ACTCC 3786145 1.468771 17.546661 140-149
CATCA 3258715 1.4652389 31.355536 45-49
CTTTA 2458195 1.4588969 41.996033 45-49
TACGC 3900250 1.4494334 25.278482 140-149
GGGGT 5057795 1.4256364 121.618935 7
GTTAC 3100320 1.425076 15.088809 50-59
CTGGT 3746205 1.4232029 31.153448 35-39
GTGAC 3975620 1.4153366 14.549392 210-219
CTCAA 3103160 1.3952957 18.480812 150-159
GGCAG 5026130 1.385836 13.59618 150-159
TAGCA 3196830 1.3769895 11.517632 130-139
GAACT 3195570 1.3764467 15.0625105 190-199
CAACT 3054305 1.3733286 16.793684 130-139
CTACG 3691615 1.3718992 16.488432 130-139
GTCCA 3690690 1.3715556 28.257074 180-189
GAGAG 4285085 1.3694394 26.250296 220-229
GGCCT 4422295 1.3583049 68.82358 3
TTATT 1847195 1.3559595 21.78425 240-247
CGACA 3886010 1.3532851 16.406372 70-79
CAACC 3721585 1.3528956 15.2984495 140-149
ATACA 2595190 1.3524977 22.47075 35-39
AGCAG 4034945 1.3460835 12.653892 50-59
AACCC 3693080 1.3425335 19.939775 140-149
TGGAA 3251240 1.341557 19.302755 120-129
CGTGT 3509040 1.3331027 23.452953 230-239
AAGCT 3094575 1.3329446 15.983898 200-209
ATCTA 2389790 1.3290691 20.21024 110-119
ACAGT 3080990 1.327093 12.228271 120-129
TCACT 2758920 1.3237977 14.023496 190-199
CCATA 2937255 1.3206986 14.175758 160-169
CAATT 2369835 1.3179711 23.983429 180-189
ACATG 3037455 1.3083409 14.550199 190-199
ACAGA 3235695 1.3060452 20.412096 140-149
CACGT 3508950 1.3040162 16.483936 180-189
TCCGT 3281025 1.3011754 13.795723 150-159
TCAAG 3011390 1.2971138 22.706657 150-159
GTGCG 4407465 1.2968432 16.932371 240-247
TGTGT 2757960 1.2959522 15.425787 240-247
ATTAT 1882805 1.2951466 9.657029 50-59
CCTTT 2515855 1.2882159 48.074177 45-49
GTGGC 4374020 1.2870024 14.0346155 40-44
AATTC 2305400 1.2821361 23.934536 180-189
ATATT 1860150 1.2795626 26.210777 240-247
TTACA 2287860 1.2723813 9.36629 120-129
AAGAG 3290610 1.2723776 18.328442 150-159
TGTCC 3202045 1.2698538 52.881157 15-19
AGAGC 3804015 1.2690437 24.966497 220-229
ACATA 2434820 1.2689198 21.1288 130-139
TGCGA 3556315 1.2660623 14.156274 240-247
CCTAC 3251475 1.2613548 17.347038 190-199
CCAGT 3380145 1.2561489 18.444265 190-199
AACCA 2974855 1.2534512 17.404007 190-199
ACACA 2960955 1.2475944 7.9768686 130-139
CGGCT 4037890 1.2402351 21.755064 230-239
AGACC 3525370 1.2276938 97.36639 9
CTTCT 2379535 1.2184147 22.898829 30-34
TGGAC 3418885 1.2171367 15.832199 80-89
ACGTC 3248165 1.2071018 18.218145 180-189
GATTG 2740200 1.2065985 23.064352 100-109
AGTCG 3385410 1.2052194 15.423608 150-159
ATCCG 3222315 1.1974953 23.37654 70-79
TACCA 2652905 1.1928444 10.724006 45-49
TGAGT 2696970 1.1875631 20.880533 90-99
ATTAG 2198930 1.171516 25.428543 110-119
ACGCA 3362610 1.1710134 21.470295 140-149
GTCGA 3283200 1.1688322 11.988378 150-159
AAGGC 3495360 1.1660744 28.722477 25-29
GAAGT 2817285 1.1624944 13.551375 140-149
GGGTG 4105415 1.1571897 14.545867 60-69
ATCAT 2067010 1.1495568 15.303636 110-119
GATCC 3089350 1.148082 23.216898 70-79
GGACT 3223845 1.1477017 12.73921 90-99
ATTCC 2381520 1.142712 22.213795 180-189
AGGCC 3957855 1.1391712 14.180557 2
CTTCG 2867860 1.1373241 102.52918 3
TCGGA 3193605 1.1369361 91.29547 5
GATCT 2469135 1.1349491 9.279232 220-229
CGCTG 3682520 1.1310837 12.335966 190-199
CCGAT 3012705 1.1195989 24.181416 160-169
TCGAG 3133820 1.1156523 11.641519 150-159
GCAAG 3334565 1.1124322 16.805641 25-29
ACAAG 2753010 1.1112158 17.54329 80-89
AGCTC 2985060 1.1093252 12.116817 210-219
TTGGG 3036120 1.1049515 14.615856 100-109
AGGTC 3103100 1.1047161 18.11829 10-14
GAACC 3154060 1.0983869 10.172503 190-199
CGCAG 3804655 1.0950763 16.800997 140-149
CACAT 2424690 1.0902305 14.006778 130-139
GACCC 3623980 1.0888445 41.79807 10-14
CTTGA 2366120 1.0875978 22.148708 90-99
GTATA 2040940 1.0873442 10.168719 240-247
AAAAA 1914955 1.0839536 5.2826104 190-199
GCTTC 2728055 1.0818808 26.842245 2
TTGAG 2454300 1.0807076 20.617044 90-99
TTCGG 2838685 1.0784314 98.318054 4
ATGGG 3156420 1.0764619 20.030823 100-109
GCCCC 4135490 1.0720174 12.266398 80-89
ATCAA 2041555 1.0639677 12.214991 110-119
ATGAG 2574675 1.0623864 20.54468 60-69
CTCCG 3309195 1.0610185 10.237502 140-149
ACACC 2914635 1.0595478 13.064225 40-44
GCACC 3514550 1.0559658 10.940155 130-139
TAGAC 2438895 1.0505196 16.652208 170-179
CCCCT 3131980 1.0482635 9.4625 70-79
TACAG 2428005 1.0458289 8.708908 120-129
CAGCA 3002775 1.0457026 12.458667 45-49
GAGCC 3617205 1.0411235 18.350903 220-229
AGATC 2415750 1.0405502 12.499041 210-219
CGTGA 2917615 1.0386825 20.906849 150-159
CTATA 1867125 1.0383917 13.32989 50-59
GAAGC 3110620 1.0377226 12.489481 200-209
GACAG 3099195 1.0339113 15.724331 70-79
CGAGG 3742260 1.0318393 15.10998 220-229
TGGCT 2707570 1.0286201 30.656141 40-44
TTTCA 1731440 1.0275803 7.818299 100-109
GGAGA 3211225 1.0262523 81.61648 7
GGGAG 3866385 1.0212502 14.383851 5
GCCAA 2931820 1.0209928 15.512789 180-189
ATGGT 2316730 1.0201311 9.412318 120-129
AGCCG 3542535 1.0196316 19.438044 220-229
AACTC 2255075 1.0139652 16.946333 190-199
TGAGG 2970215 1.0129588 12.000796 220-229
GTAGC 2834065 1.0089384 11.400324 50-59
ACCGC 3342510 1.0042754 11.124479 220-229
GCTCT 2526220 1.0018378 16.671333 210-219
TTATA 1452545 0.99917865 13.28464 110-119
AAACT 1916685 0.998891 16.760208 130-139
ACTCA 2220680 0.9985 12.93981 190-199
ATGAC 2314510 0.9969425 11.095263 160-169
TAGTT 1748195 0.9939102 14.497328 50-59
CCGTC 3054890 0.9794813 10.317556 140-149
CCGAG 3394875 0.9771313 18.243162 220-229
ATGAT 1831800 0.9759214 8.922032 120-129
AAGGT 2361845 0.9745665 20.387888 15-19
GATAC 2240820 0.9652016 24.319384 35-39
AACTG 2237770 0.96388793 12.415371 60-69
TTTAG 1690010 0.96083 30.020884 45-49
TTTAT 1305230 0.95812243 11.463581 240-247
GCAGT 2687175 0.95664495 6.4216275 50-59
CGAGT 2668580 0.9500251 14.891782 160-169
GGGAC 3445290 0.94995695 9.798658 90-99
GTAGA 2298520 0.9484368 16.292927 170-179
CATAC 2106725 0.9472616 14.274186 130-139
ACAGG 2834730 0.9456839 15.277798 70-79
ACCAA 2223895 0.9370352 16.408184 130-139
GCCAT 2518570 0.93596554 7.6665225 60-69
CGGAC 3249125 0.9351807 10.591712 220-229
CAGAA 2314755 0.93432003 18.194849 140-149
TAACA 1788595 0.93213624 8.959103 120-129
GGTAT 2111695 0.92984754 9.2011385 110-119
CCAAC 2531695 0.9203389 13.394853 130-139
TACAC 2041565 0.9179632 22.194172 35-39
TATTT 1248200 0.91625875 11.468455 240-247
AGCCC 3033575 0.9114542 11.406325 80-89
GTTAT 1602995 0.9113589 10.71362 110-119
AAGTT 1707630 0.9097678 17.536266 140-149
TCATA 1626245 0.9044276 8.784277 110-119
GAGAT 2189245 0.9033467 12.0850935 210-219
TATGG 2048045 0.9018204 11.692213 35-39
AATCA 1722555 0.89771914 13.307836 110-119
CCCAG 2960490 0.8894953 14.425047 80-89
GATGA 2146250 0.88560575 7.044551 60-69
AGTCT 1924410 0.88456374 15.773164 8
TGGTT 1881060 0.8839011 8.927288 50-59
GACTG 2450035 0.8722222 12.359147 90-99
GTTTC 1774435 0.8703864 5.47596 100-109
CGTAT 1880965 0.86459404 7.334901 240-247
CCCCA 2755495 0.8642327 16.586117 80-89
AGAAG 2234505 0.8640143 15.822251 140-149
TCCTT 1681145 0.86081177 10.458455 1
GTCTG 2245630 0.8531266 14.347415 1
CGGAG 3093345 0.8529164 70.70353 6
ACGCT 2292220 0.851848 15.183937 190-199
GCCTA 2286685 0.84979117 16.66248 190-199
ATAGT 1583665 0.8437235 15.458625 120-129
TCTTC 1640270 0.83988214 45.26089 2
CATGG 2354235 0.838117 8.5242815 200-209
GGTTC 2192075 0.8327809 11.3363 160-169
GTCTA 1809850 0.8319058 9.425196 30-34
TTACC 1730175 0.8301806 9.734114 45-49
GGTAG 2432950 0.8297305 11.864141 120-129
TTATG 1459245 0.829632 12.122048 50-59
GATCA 1918765 0.826481 9.087899 110-119
ATCCA 1823845 0.82006824 9.553068 180-189
ATATA 1267745 0.8171937 11.178623 130-139
TGGCA 2288375 0.8146707 5.753047 100-109
GGTGC 2750105 0.8091851 11.647103 60-69
GCTTG 2125935 0.80765384 16.849396 90-99
TCATC 1677665 0.80498505 10.332586 110-119
CTATC 1676530 0.80444044 7.9997983 110-119
ACAAA 1639080 0.8004736 12.9496355 130-139
ACATC 1770010 0.795862 5.6264696 110-119
ATATG 1493265 0.7955614 5.5806932 50-59
CATTA 1428850 0.7946474 12.728912 110-119
TGATG 1795480 0.7906078 12.30934 120-129
TATAC 1403500 0.7805491 9.325498 130-139
CCTCC 2314610 0.7746926 10.169576 140-149
GTTCA 1660810 0.7633989 13.838209 160-169
CTGAC 2036385 0.7567731 9.508825 220-229
TCAAC 1666385 0.7492684 7.5122137 110-119
AACGC 2150300 0.74883205 15.196472 180-189
CCAGC 2488915 0.7478081 6.52793 80-89
TATGA 1403410 0.74768966 5.0887747 50-59
ACGCC 2478900 0.744799 13.596471 180-189
ATCTT 1252085 0.74309117 12.512032 3
GGGAT 2164875 0.7383065 13.251736 100-109
TATTG 1288230 0.73240393 6.877292 240-247
TTGGA 1660930 0.7313611 6.5184126 60-69
CAACG 2093970 0.7292153 15.494168 180-189
GCGGA 2640795 0.7281365 8.85674 220-229
TTTAC 1218430 0.7231175 13.255832 45-49
GGTTA 1640355 0.7223013 7.9192524 50-59
TTTCT 1136850 0.719999 9.620318 4
ATAAA 1184855 0.71571124 7.662277 130-139
GACCG 2481825 0.7143323 8.945679 210-219
GACCA 2041595 0.710976 6.1546326 170-179
ATGCC 1904925 0.70791924 8.16087 50-59
GGGCA 2563000 0.70668644 13.588521 150-159
GGATA 1711470 0.70620275 15.750277 35-39
CCCCC 2608790 0.7059354 13.161918 80-89
TGCCA 1893755 0.70376813 7.354316 60-69
AATAC 1339005 0.69782984 11.512695 130-139
GATTA 1304715 0.69510823 5.6370254 50-59
CGCCG 2789110 0.6926112 10.713598 220-229
GAAAT 1387270 0.6925918 6.980552 110-119
CACAA 1642645 0.69212633 8.422573 130-139
TCCGG 2251775 0.69163114 11.281984 70-79
CAGTC 1849540 0.68733674 5.670494 210-219
TATAG 1289685 0.6871008 6.4790196 120-129
CATCC 1762980 0.6839183 6.353544 110-119
AAATA 1106175 0.6681846 12.7141695 130-139
CAAAC 1584965 0.66782284 13.55409 130-139
CCCGT 2066975 0.66272867 10.085546 140-149
GCCGC 2667380 0.66238236 11.85187 220-229
AGATG 1600070 0.6602358 6.0630455 120-129
AGGCA 1968395 0.6566692 6.5389485 80-89
CACGC 2174990 0.6534876 12.360077 190-199
GCGAC 2267110 0.65253186 7.1881824 70-79
TACTT 1088095 0.64576584 10.997369 1
GACGA 1928415 0.6433315 9.806169 220-229
ACTTC 1325685 0.63609636 29.68245 4
TCAAT 1120645 0.6232408 8.087268 110-119
CACCG 2066900 0.6210113 8.964998 45-49
AAGCA 1532780 0.61868626 5.811125 120-129
AATAA 1024185 0.61865854 7.142513 130-139
AATGG 1482465 0.6117086 6.749367 120-129
TAGCT 1313520 0.60376537 12.494812 50-59
CCGGC 2408435 0.59807926 9.187889 70-79
CCCTA 1537040 0.5962687 11.747018 110-119
TCCAC 1534425 0.59525424 10.0947485 180-189
AGTAA 1192165 0.595186 5.407133 50-59
ACGAC 1704360 0.5935355 9.152041 220-229
TTCAA 1060435 0.58975536 5.8712096 200-209
CACGA 1688650 0.58806455 8.66471 180-189
TGATT 1033005 0.5872996 6.0870433 50-59
GGTGA 1713445 0.5843513 6.7101173 120-129
GGCCC 2353140 0.584348 10.111828 70-79
ATTTC 983420 0.583643 6.741163 240-247
ACCTA 1294610 0.5821046 7.417355 130-139
ACCCG 1929915 0.5798535 8.766245 140-149
ATTAA 895150 0.5770174 6.9640822 110-119
CTTTG 1176135 0.57691145 7.2811546 45-49
GTTCC 1454235 0.5767145 6.793366 140-149
CCGTA 1550780 0.5763098 6.3343883 230-239
GGGGA 2163405 0.5714325 9.260767 4
TTAGC 1212295 0.5572369 9.723526 45-49
CGGTT 1461540 0.5552467 11.222159 160-169
TCAGG 1557435 0.554453 5.3280554 150-159
TAATA 854865 0.55104953 7.796451 130-139
CTGTT 1109930 0.544437 5.9516973 240-247
GAAGA 1390665 0.53772736 6.4377 15-19
ACATT 966460 0.53749156 6.604999 110-119
AAGAT 1076235 0.53730816 5.8279476 15-19
CGGCA 1865000 0.53679436 10.575995 70-79
GGAGG 2005100 0.5296185 5.262304 6
CTGCG 1723105 0.5292505 11.509995 25-29
TATGT 928850 0.52808386 6.9012136 140-149
CCACG 1748075 0.5252186 7.604718 180-189
GCTAC 1412810 0.5250366 7.8442693 50-59
GTCCT 1317955 0.52266914 6.02018 45-49
GGGTT 1403515 0.51078874 6.3412232 100-109
AAATC 973250 0.5072146 7.508769 110-119
GTAAC 1158425 0.49897522 7.0993695 120-129
AGTAT 931975 0.4965249 5.3435154 110-119
ACCCC 1572230 0.4931138 8.72253 140-149
TGACG 1375370 0.48963717 8.60261 220-229
TGGCC 1584010 0.48652762 14.721722 2
CGCGG 2031610 0.483296 6.365172 220-229
TATCC 1006355 0.48287395 5.344911 110-119
AACAT 926250 0.48272032 6.044329 110-119
GGTTT 1023040 0.48072162 5.816261 100-109
CATAA 913570 0.47611207 6.1685376 110-119
CCGGT 1497655 0.4600037 8.7243185 160-169
CAATC 1019415 0.4583667 6.0077047 110-119
ATTCT 754430 0.44774136 7.176863 2
ATGCT 972750 0.44712892 6.790597 50-59
CCGCG 1788170 0.4440508 6.600395 220-229
TAAAT 682455 0.43991333 7.665826 130-139
TGCTA 948530 0.43599612 5.9534125 50-59
GCCTT 1077950 0.427489 8.717843 1
TGCGC 1388375 0.42643842 8.469553 25-29
GCATG 1188225 0.42301282 8.466127 60-69
GGTCA 1179585 0.41993698 5.3884335 160-169
CACTT 866435 0.41573682 7.0289907 3
ATGGC 1149900 0.409369 6.905543 1
CTAAC 907595 0.40808833 5.631245 110-119
TACCT 829730 0.39812487 5.0745955 2
CCTAA 877050 0.3943542 7.7651305 110-119
GCGCT 1281435 0.39359188 7.647096 25-29
CCGGG 1650500 0.39263442 5.3089213 80-89
ACGAG 1176850 0.3926047 7.4642057 180-189
AGCTT 851755 0.39151302 6.936188 1
CATGC 1050980 0.39057124 6.9733887 60-69
TTTGA 680955 0.38714683 6.0912066 45-49
GGTAA 926805 0.38242695 7.485221 120-129
GAGGC 1341695 0.36994055 5.532796 35-39
TTCTT 570405 0.3612535 18.852549 1
TGCTG 914800 0.3475373 14.421093 1
TGGGC 1167055 0.3433918 9.512525 1
ATGTG 777615 0.34240898 5.203205 140-149
CGAGC 1135980 0.32696387 6.2941356 180-189
GCTTT 632255 0.3101304 5.1575103 45-49
CTCTT 603740 0.30913842 8.651391 1
GCGAG 1093930 0.30162522 5.3609204 240-247
TCACA 669695 0.3011197 5.319351 5
GACTT 650070 0.2988076 6.823765 1
CGGGA 1081525 0.29820484 5.0340543 80-89
CGCTT 748505 0.29683906 5.7825027 1
ATTGT 521315 0.29638588 5.677081 240-247
AACTT 525125 0.2920455 10.497201 3
CTTTC 570045 0.29188526 5.0890756 45-49
CTCGG 950130 0.29183176 5.005109 6
TTCCT 554880 0.28412017 6.3800864 4
GAGGT 825200 0.28142527 6.6128774 7
CCCTT 660740 0.27353185 5.437964 3
TAACT 476710 0.26511976 7.4493957 2
TTGGC 693040 0.26328954 7.341007 1
GTCTT 536680 0.26324943 11.834748 1
AATCT 467760 0.26014227 7.6218224 2
TGTAA 481045 0.2562846 7.9367704 25-29
CGGGG 1117100 0.2545739 9.244584 3
CCTCG 735485 0.23581663 5.5282907 5
TGCTT 431105 0.21146335 11.079778 1
TCGGT 530250 0.20144476 5.9661374 7
TCGCA 443745 0.16490708 5.5000777 7
TAGGC 426380 0.15179299 6.815392 1
TTGCT 253285 0.12424002 5.7974195 4
Produced by FastQC (version 0.10.1)