FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_6_SL248352.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_6_SL248352.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11572873
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 55149 0.47653681155923855 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 40168 0.3470875382456889 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 39261 0.33925024494781897 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 38504 0.33270908615345557 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 38231 0.3303501213570736 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 37571 0.3246471295416445 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 37481 0.3238694488395405 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 34271 0.2961321704644992 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 33913 0.2930387294494634 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 33747 0.2916043405989161 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33655 0.29080937810343205 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33636 0.2906452010663212 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33433 0.2888910990382423 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 32921 0.28446695993293974 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 31233 0.2698811263201454 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30764 0.26582854577251475 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30647 0.2648175608597796 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30352 0.26226849633621657 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 30045 0.25961574105237306 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29115 0.2515797071306321 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 29055 0.2510612533292295 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 28954 0.25018852276353504 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 28154 0.24327580541149982 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27663 0.2390331251366882 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27317 0.23604337488193294 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26761 0.23123903632226847 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24976 0.21581503573053987 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24316 0.2101120439151108 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23900 0.2065174308920525 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23852 0.20610266785093034 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23734 0.20508304204150518 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23195 0.20042559872557142 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 23037 0.19906033704854448 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22911 0.19797158406559892 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22697 0.1961224321739295 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22693 0.19608786858716934 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22510 0.19450658449289127 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21847 0.18877766998739207 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21810 0.18845795680986044 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21807 0.18843203411979031 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21711 0.18760250803754608 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21378 0.1847250894397614 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20396 0.17623972889013817 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 20057 0.17331046491221325 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 19975 0.17260191138362962 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 19760 0.17074411859527017 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19436 0.1679444680676959 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 18816 0.1625871121198686 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18591 0.16064291036460868 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 18560 0.16037504256721732 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 18558 0.16035776077383723 No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG 18396 0.15895793551005008 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18379 0.15881104026631937 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18150 0.15683227492429924 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 17930 0.15493127765248957 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17711 0.15303892127736993 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16815 0.14529667784309047 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 16713 0.144415306380706 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 15800 0.13652616770269577 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15772 0.13628422259537454 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15574 0.13457332505074582 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15348 0.13262048239879587 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14826 0.1281099343265929 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14391 0.12435114426642374 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14370 0.1241696854359328 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13552 0.11710143194347679 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12914 0.1115885398552287 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12197 0.10539301692846711 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12135 0.10485728133368438 No Hit
TAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 11677 0.10089975064964422 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 23713895 7.7401075 66.77846 35-39
TATTA 11757755 7.472375 128.93062 240-247
TGGAT 17916170 7.280914 80.14129 35-39
TCACC 18365965 6.462904 75.088554 160-169
TCTCC 16251030 6.111734 85.8742 20-24
CTGTG 17127490 5.97458 87.98734 25-29
TCCCT 15527660 5.839687 73.083206 15-19
AGTGG 18497070 5.814496 40.386604 100-109
CTGAG 17377755 5.672021 50.55957 15-19
TGTAT 10675955 5.6089163 59.637306 240-247
TTACT 9945180 5.425251 113.85725 240-247
ATTAC 9965095 5.0865 107.2101 240-247
TGGGT 14743555 4.9531436 46.18499 100-109
CCTGA 14429005 4.890074 51.995075 15-19
CTCTG 13141790 4.759958 74.6108 35-39
TTCAC 10794985 4.729689 76.411095 40-44
ACACG 14721445 4.668313 49.692184 230-239
CTCCA 13220360 4.652188 41.587456 80-89
GACAC 14588115 4.626032 46.736397 230-239
TGAGA 11863825 4.5112395 95.700775 15-19
ATTCA 8608795 4.3942018 114.67385 40-44
AAGGG 14862620 4.371545 40.062157 90-99
TACTG 10340650 4.3633847 80.118866 240-247
TCTGG 12348315 4.307461 136.80135 35-39
GAAGG 14326860 4.2139616 31.845367 90-99
TGGAG 13374640 4.2042766 39.514446 90-99
CCCTG 14352460 4.17521 85.36593 10-14
AGCCT 12312975 4.1729383 52.111496 30-34
CAGCC 15223010 4.143649 56.368557 30-34
CCAGG 15785705 4.1382003 53.900906 80-89
ACCAT 10053990 4.1217294 68.44573 160-169
GAGAC 13376035 4.0850925 89.01721 8
TCAGT 9645360 4.069997 96.42949 7
ACTAC 9855285 4.0402684 36.438446 130-139
TACTA 7846340 4.0050206 29.898287 130-139
CACCA 12145695 3.999142 57.95287 160-169
TCCAG 11781255 3.9927359 41.05881 80-89
GTATT 7560085 3.9719055 69.425156 240-247
GGGTC 14588095 3.9362323 126.226776 8
ACTGT 9089960 3.835638 68.90639 240-247
CACCT 10849160 3.817773 43.96762 40-44
TGAAG 9965670 3.7894628 40.66655 150-159
CTGGG 14035675 3.7871752 133.66415 3
GACTC 11134965 3.773704 52.2418 20-24
AGACT 9505500 3.7530215 69.787056 15-19
GATTC 8889810 3.7511818 60.21197 40-44
CAAGA 10078000 3.7231536 45.689682 180-189
GGACA 11864300 3.623403 28.596422 220-229
GCTGG 13337200 3.5987093 33.65304 90-99
ACTGG 10946425 3.5728633 44.810818 60-69
CCTGT 9687560 3.508835 61.679874 25-29
ACTCT 7873500 3.449677 68.94669 20-24
GAGTG 10854495 3.4120767 39.347946 90-99
TATCT 6225160 3.395922 49.98054 200-209
GGATT 8246255 3.351178 57.675507 40-44
AGACA 9047045 3.3422837 38.992245 170-179
TCCTG 9179435 3.3247924 63.244972 20-24
AGCTG 10160700 3.3164062 37.94482 60-69
CTCCT 8630680 3.2458508 92.604195 20-24
CTGCA 9518215 3.2257786 46.164253 25-29
GGTCC 11478605 3.215927 141.84253 9
CATCT 7252705 3.1776834 32.932354 170-179
GTCTC 8636635 3.1281903 33.88333 30-34
CCATC 8884565 3.12644 36.118595 45-49
GTCCC 10510580 3.057586 74.82502 10-14
AAGAA 7043820 3.0315998 39.334274 180-189
GGAGT 9643865 3.031519 37.84225 90-99
CCTCA 8609810 3.0297549 80.909096 5
AGTAG 7864140 2.9903524 29.972658 50-59
GGCTG 10896530 2.9401557 34.78607 90-99
AGGGA 9984165 2.9366443 41.46847 80-89
GAGCT 8937235 2.917073 30.954622 60-69
TGTGC 8144795 2.8411477 58.372467 25-29
GGAAG 9538310 2.805505 31.090456 80-89
ATCTG 6624035 2.795106 37.56299 200-209
GTGAA 7343820 2.7924998 39.628452 150-159
CAGGG 10983975 2.7731416 37.13092 80-89
GACAA 7464750 2.7577302 25.109861 180-189
ATCTC 6257335 2.741574 48.474926 170-179
GAACA 7398865 2.7333903 35.7375 210-219
ACCTT 6233590 2.7311707 72.146774 40-44
GTGTA 6698995 2.7223902 36.758167 230-239
CTATG 6420100 2.7090526 28.311962 50-59
ATGAA 5827080 2.6803045 28.26558 210-219
GGGAA 9002445 2.647891 34.67294 80-89
AGAAC 7047595 2.6036193 31.066553 190-199
GAGTC 7959625 2.5979855 28.924871 150-159
CACGG 9843975 2.580584 44.473133 230-239
TGCAA 6473655 2.5559695 36.437572 200-209
CCCTC 8419145 2.5430498 27.180304 20-24
CGCCA 9340835 2.5425422 39.869762 70-79
ATCAG 6422365 2.535719 28.212793 45-49
TATAT 3970645 2.5234532 25.274906 240-247
AGGGC 9993995 2.5232 33.046787 150-159
CCAAG 7930615 2.514875 39.94842 180-189
CAGTG 7650225 2.4969988 74.539955 8
CTACA 6065710 2.486696 19.51022 190-199
AGGCT 7601340 2.4810429 20.312191 80-89
ACGGC 9387420 2.4608986 49.151623 230-239
GTGGA 7728150 2.4293199 27.039999 100-109
CACTG 7133735 2.4176643 20.345123 30-34
TCTGT 5339755 2.4080555 25.36873 210-219
GCAGC 9173715 2.4048762 35.09902 30-34
CTGTC 6608415 2.3935688 49.95996 30-34
CTACT 5437875 2.3825378 23.419743 50-59
TGAAC 5982635 2.3621016 38.590416 210-219
CCGCC 10012110 2.3392735 34.00327 70-79
CTCTC 6214130 2.3370275 97.700096 20-24
TATGC 5522770 2.3304114 21.81276 50-59
TCCAA 5672480 2.3254874 44.150608 180-189
CAAAT 4868465 2.3251991 36.022446 200-209
GGGCT 8583040 2.3159184 42.670055 90-99
ACAGC 7302390 2.3156588 27.397392 210-219
CTTCA 5263610 2.3061857 50.609413 45-49
GTATC 5445290 2.2977176 23.26897 200-209
GTGGT 6804950 2.2861443 32.069393 120-129
GGCCG 10486880 2.272646 25.370062 230-239
TGGTG 6752235 2.2684348 35.19976 35-39
CAGGC 8612840 2.2578435 33.411594 70-79
CAGTA 5694415 2.2483048 22.10365 45-49
AATGA 4885190 2.2470596 26.519064 210-219
AAATG 4878570 2.2440145 34.296906 200-209
ACAAC 5830410 2.2365088 20.312204 180-189
GCCTC 7650145 2.2254696 71.21703 4
GCTCC 7603280 2.2118363 34.128834 80-89
GTAGT 5423675 2.2041156 26.472462 120-129
GCCTG 7773870 2.1779823 30.749195 210-219
TACAT 4263830 2.176394 18.44892 190-199
GGGGG 10738860 2.1586084 95.670044 5
GCTAT 5104220 2.1537983 34.73993 50-59
TATCA 4211400 2.1496322 17.684246 170-179
GTGGG 8255075 2.145202 30.487251 100-109
CCTTC 5650285 2.1249752 55.047455 45-49
TGCAG 6440005 2.1019886 44.37787 30-34
GCAGA 6844930 2.0904682 24.906466 140-149
TCTAT 3826120 2.0872083 20.996508 110-119
GCTGT 5976965 2.0849438 19.382341 230-239
CCTCT 5534720 2.0815134 49.306293 35-39
AACAG 5558720 2.0535789 34.426556 210-219
AACTA 4283615 2.0458722 37.68905 130-139
TGCAC 5979100 2.026352 27.396723 25-29
AGCAC 6366265 2.018804 21.363585 130-139
TGAGC 6180415 2.0172594 18.317959 200-209
CTCAC 5730575 2.0165646 55.222668 20-24
TGTGA 4935010 2.005528 19.686779 210-219
CAGAG 6565410 2.0051017 32.94478 170-179
TGGTA 4928455 2.0028644 31.844936 120-129
ATATC 3922475 2.0021555 14.113348 160-169
GGCTC 7116440 1.9937922 20.654522 40-44
ATGGA 5187055 1.9723864 12.212093 100-109
CATAT 3861150 1.9708534 14.918039 160-169
AGAGT 5177935 1.9689182 31.39709 150-159
TCCGC 6717330 1.9541084 42.11955 70-79
TCTCT 4138855 1.9380293 33.62018 35-39
CTGTA 4592375 1.9378182 25.005222 190-199
CCGTG 6906615 1.935006 20.279072 230-239
TTCAG 4584060 1.9343091 47.552574 45-49
CAGAC 6096390 1.9332242 26.927847 140-149
GGTCT 5509415 1.9218484 35.197575 15-19
TCCAT 4329915 1.8970988 27.971088 45-49
CTATT 3473975 1.8951076 20.971245 110-119
GGTGG 7273520 1.8901305 18.776428 40-44
GCACA 5952585 1.8876222 18.925865 130-139
GGATC 5780585 1.8867567 18.569849 70-79
CCAGA 5939990 1.8836285 34.45862 170-179
GCCAG 7182255 1.8828177 35.66259 70-79
ACTAT 3688615 1.882786 17.414669 130-139
TGGGG 7239800 1.8813679 124.511986 4
ACCAG 5901905 1.8715514 14.242038 190-199
AGAGA 5253850 1.8693011 28.745142 170-179
GTCAC 5511575 1.8679049 38.287018 160-169
CAGTT 4417110 1.8638625 25.456854 190-199
AGGAC 6067920 1.8531662 20.892683 220-229
TGGGA 5888920 1.8511636 16.725428 100-109
GCTGA 5668115 1.850047 27.511744 200-209
ACCCT 5242540 1.8448274 43.665787 10-14
GATGG 5832465 1.8334172 20.61505 100-109
ATACT 3589520 1.8322048 27.777414 130-139
TGTCT 4058530 1.8302649 51.154446 30-34
CCTGC 6290915 1.8300619 49.16129 20-24
TAGTA 3700890 1.8193192 17.831337 120-129
TAGTG 4473560 1.8180006 26.976498 120-129
TTCCA 4130900 1.8099028 23.382502 180-189
GCCGA 6893345 1.8070805 19.782284 220-229
GAGGA 6111710 1.7976384 27.318052 220-229
TCAGC 5300925 1.7965144 11.631045 45-49
CACAG 5616445 1.7810292 15.823959 140-149
AGTCA 4488085 1.772014 29.528101 160-169
TACAA 3702515 1.7683367 18.464882 130-139
CAAGG 5774370 1.7635151 17.245148 80-89
GCAAA 4745865 1.7532827 32.971066 200-209
AGTTC 4143970 1.7486072 22.671537 190-199
CATGA 4426180 1.7475723 24.18195 60-69
AGCTA 4398385 1.736598 26.614447 50-59
ACCTG 5114100 1.7331983 31.00756 25-29
CTGAA 4360470 1.7216284 24.055515 200-209
TGACC 5060625 1.7150753 14.554065 210-219
GTGCA 5254465 1.7150335 51.23496 25-29
GGAGC 6740255 1.7017231 13.033226 200-209
CTCAG 4993380 1.6922855 77.63022 6
TTCTG 3745545 1.6891187 42.45775 30-34
CCTGG 6021345 1.6869824 35.1623 2
ATGCA 4258110 1.681214 16.487633 60-69
ATTGG 4112765 1.6713777 21.359863 100-109
AACAC 4336055 1.6632836 22.90314 190-199
GGATG 5266200 1.6554135 22.064219 100-109
AGGGG 6710450 1.6316586 33.503563 90-99
TCTGC 4463270 1.6165969 33.738922 200-209
TCTCA 3663005 1.6049005 27.09763 100-109
CAGCT 4720495 1.5998032 13.118475 50-59
CGATT 3784820 1.5970587 35.370956 160-169
GGGGC 7649280 1.5965083 28.94731 90-99
GCCGT 5689255 1.5939418 29.604828 230-239
GGGCC 7336980 1.590021 27.837475 2
CTCCC 5225135 1.5782813 14.016125 190-199
AGTTA 3204410 1.5752546 16.809685 50-59
GCACT 4607555 1.5615273 26.50498 25-29
ACAAT 3258690 1.5563639 23.729603 180-189
GTTCT 3450300 1.5559729 23.823738 190-199
TCTGA 3674750 1.5506132 21.224789 220-229
TTCTC 3301625 1.5459942 22.651552 190-199
CGTCC 5267250 1.5322722 19.26926 180-189
TTAGT 2907090 1.5273223 26.442032 110-119
CCATG 4500235 1.5251559 15.626922 160-169
GAGCA 4961650 1.5153071 7.7831426 200-209
GGCTT 4309005 1.5031097 21.42475 25-29
AGTGA 3950620 1.5022299 86.49688 9
CGGCC 6674430 1.5018778 31.612587 230-239
CTTTA 2751015 1.5007216 43.349552 45-49
GTTAC 3550135 1.4980301 16.558813 50-59
GTCCG 5326725 1.4923729 34.40485 70-79
CATCA 3631460 1.4887519 32.449856 45-49
ACTCC 4125245 1.4516559 17.361378 140-149
CTGGT 4138510 1.4436358 32.37672 35-39
TAGCA 3642665 1.4382201 12.126092 130-139
CTCAA 3483605 1.4281372 19.097998 150-159
TACGC 4195950 1.4220319 24.865396 140-149
GTGAC 4346835 1.4187872 15.59201 210-219
GGCAG 5555870 1.4026996 13.540033 150-159
GGGGT 5396020 1.4022348 119.16769 7
GTCCA 4134130 1.4010807 29.17351 180-189
AAGCT 3540570 1.3979102 17.50604 200-209
CAACC 4239975 1.3960718 15.593245 140-149
CAACT 3375505 1.3838207 18.19887 130-139
AACCC 4169945 1.3730134 20.707663 140-149
GAGAG 4650865 1.3679599 26.370668 220-229
AGCAG 4472340 1.3658698 13.026388 50-59
ATCTA 2673865 1.3648255 21.574083 110-119
GAACT 3423990 1.3518814 14.796804 190-199
CTACG 3988380 1.3516852 16.39901 130-139
CGTGT 3870620 1.3501878 24.20895 230-239
CCATA 3276340 1.343167 15.377023 160-169
CAATT 2628020 1.3414247 24.878922 180-189
CACGT 3947290 1.3377597 16.882116 180-189
TCAAG 3365430 1.3287604 24.041075 150-159
GGCCT 4735530 1.3267394 68.41628 3
AAGAG 3724540 1.325178 19.72766 150-159
TGGAA 3484945 1.3251562 19.68902 120-129
ACAGT 3351745 1.3233572 12.575276 120-129
TTACA 2589460 1.3217425 10.02868 120-129
CGACA 4167950 1.3216972 16.209366 70-79
CCTTT 2816045 1.3186202 49.669186 45-49
GTGGC 4863425 1.3122734 14.863221 40-44
AATTC 2556565 1.3049518 24.903376 180-189
ATACA 2729720 1.3037256 22.103594 35-39
TGTCC 3596325 1.3025893 54.677868 15-19
AACCA 3390885 1.3007222 17.829538 190-199
GTGCG 4808485 1.2974492 17.24993 240-247
TTATT 1909985 1.2972802 20.375292 240-247
TGTGT 2982760 1.2954736 15.264409 240-247
AGAGC 4228000 1.2912474 25.32216 220-229
ACATA 2699540 1.2893115 21.794573 130-139
ACATG 3259545 1.2869543 14.481875 190-199
CCAGT 3795770 1.2864087 19.391851 190-199
TCCGT 3540950 1.2825323 13.956267 150-159
TGCGA 3918155 1.278868 14.503808 240-247
TCACT 2916290 1.2777365 13.596666 190-199
ACAGA 3448380 1.2739481 20.057758 140-149
ATATT 2002675 1.2727545 26.926277 240-247
ATCCG 3721845 1.2613549 24.469578 70-79
GATTG 3102745 1.2609178 24.570086 100-109
AGACC 3975650 1.260717 100.96913 9
ACGTC 3679595 1.2470362 18.721434 180-189
ATTAT 1944705 1.235913 9.562509 50-59
AGTCG 3783885 1.2350429 16.623041 150-159
CCTAC 3500175 1.2316965 16.943132 190-199
ACACA 3205935 1.2297766 7.6232586 130-139
CTTCT 2602685 1.2187138 22.661943 30-34
CGGCT 4348550 1.2183204 21.150436 230-239
ATTAG 2442165 1.2005429 26.09337 110-119
TGGAC 3654255 1.1927322 15.852931 80-89
GTCGA 3653890 1.1926131 12.917079 150-159
TACCA 2900740 1.1891862 10.721565 45-49
GATCC 3507100 1.1885766 24.286047 70-79
TGAGT 2898850 1.1780574 20.865099 90-99
TCGGA 3604090 1.1763586 95.090225 5
ATCAT 2292735 1.1702846 15.842049 110-119
ACGCA 3686240 1.1689423 21.443045 140-149
GAAGT 3067270 1.1663344 13.692786 140-149
ATTCC 2656740 1.1640179 22.90566 180-189
CTTCG 3206040 1.1612281 106.40362 3
AGCTC 3412020 1.1563534 12.744514 210-219
CGCTG 4119740 1.1542155 12.637086 190-199
GGACT 3530690 1.1524012 13.503352 90-99
GGGTG 4407800 1.1454314 14.466901 60-69
TCGAG 3505135 1.1440603 12.558428 150-159
TTGGG 3395825 1.140838 15.8951435 100-109
AAGGC 3729420 1.1389792 28.694424 25-29
GTATA 2316625 1.1388288 10.522583 240-247
GAACC 3551885 1.126337 10.911057 180-189
GACCC 4114715 1.1200107 42.90929 10-14
GATCT 2651360 1.1187791 9.227962 220-229
AGGCC 4252575 1.1148064 14.418527 2
GCAAG 3639730 1.1115876 16.57395 25-29
TTCGG 3178525 1.1087644 102.49553 4
GCCCC 4720385 1.1028916 12.35051 80-89
ACAAG 2983925 1.1023628 17.502558 80-89
TAGAC 2789980 1.1015575 18.079498 170-179
GAAGC 3605030 1.1009899 13.637272 200-209
CGCAG 4191305 1.0987445 16.880573 140-149
CCGAT 3232220 1.0954182 23.696726 160-169
CACAT 2669690 1.094465 14.390826 130-139
CCCCT 3600140 1.0874424 9.918193 140-149
GCACC 3991960 1.0865973 11.388779 130-139
AGGTC 3320480 1.0837896 18.19997 10-14
CTTGA 2546550 1.0745531 22.073439 90-99
TTGAG 2643205 1.0741663 20.597551 90-99
GCTTC 2958200 1.0714604 27.540977 2
ATGAG 2804045 1.0662428 20.884884 60-69
CTATA 2086125 1.0648243 13.81218 50-59
ATCAA 2214710 1.0577548 12.625321 110-119
ATGGG 3355865 1.0549057 19.701176 100-109
TAGTT 2005295 1.0535388 15.747612 50-59
GGAGA 3571310 1.05043 85.566 7
TGGCT 3010785 1.0502517 32.49284 40-44
GAGCC 3994005 1.0470227 18.406744 220-229
GCTCT 2885015 1.0449528 17.909958 210-219
CTCCG 3590930 1.0446215 9.9362545 140-149
TACAG 2644245 1.0440176 9.335632 120-129
ACACC 3161380 1.0409291 12.7771635 40-44
GTAGC 3188535 1.0407233 11.612102 50-59
CAGCA 3280755 1.0403591 12.441457 45-49
GGGAG 4270330 1.0383388 14.17353 5
AGATC 2620685 1.0347153 12.711381 210-219
CGTGA 3167645 1.0339049 20.944777 150-159
ATGGT 2517875 1.0232338 9.700784 35-39
AGCCG 3867355 1.0138215 19.402424 220-229
ACCGC 3720780 1.0127832 11.243348 220-229
GTAGA 2654175 1.0092545 17.850796 170-179
CGAGG 3994690 1.0085458 14.929159 220-229
GACAG 3291580 1.0052612 15.626175 70-79
TTTAG 1907350 1.0020806 31.486944 45-49
TTATA 1571350 0.9986359 13.765638 110-119
ACCAA 2596790 0.9961124 17.65736 130-139
AAACT 2082105 0.99442196 17.156057 130-139
CGAGT 3041685 0.9927922 15.327058 160-169
ACTCA 2419295 0.9918132 12.533988 190-199
TTTCA 1811740 0.98833245 7.3136315 100-109
GCCAA 3114840 0.9877459 15.131033 180-189
TGAGG 3135050 0.9854932 11.74762 220-229
ATGAC 2473600 0.9766424 10.985774 160-169
CCAAC 2965665 0.97648716 14.496978 130-139
AACTC 2381470 0.9763064 16.416136 190-199
GGTAT 2394320 0.9730226 9.548606 110-119
AAGGT 2533715 0.96344924 20.304668 15-19
CCGAG 3670330 0.96217173 18.295156 220-229
CATAC 2328260 0.9544925 14.6607275 130-139
ATGAT 1940370 0.9538657 9.142327 120-129
GGGAC 3777760 0.95377725 10.362259 90-99
AAAAA 1830680 0.953099 5.1730757 190-199
GCCAT 2785770 0.9441137 8.01002 60-69
CCGTC 3236205 0.94143 10.156691 140-149
AGCCC 3455805 0.9406578 11.684358 80-89
CAGAA 2533885 0.9361026 18.149271 140-149
CGGAC 3561630 0.9336762 10.849029 220-229
GCAGT 2857325 0.9326179 6.3588514 50-59
GATAC 2353980 0.9294132 23.766033 35-39
TTTAT 1364335 0.9266695 10.73487 240-247
ACAGG 3031320 0.9257769 15.175121 70-79
AACTG 2340955 0.92427063 11.787406 60-69
TACAC 2240735 0.9186108 22.047562 35-39
TATGG 2258035 0.91763794 12.541873 35-39
CCCAG 3368360 0.9168556 15.047454 80-89
CCCCA 3236010 0.9145916 17.116922 80-89
TCATA 1786840 0.9120598 9.011626 110-119
AATCA 1906700 0.9106478 14.083805 110-119
TGGTT 2093850 0.90940183 9.372898 50-59
TAACA 1901395 0.9081142 8.5468 120-129
AAGTT 1844055 0.90651816 17.711878 140-149
GACTG 2760365 0.9009706 13.149201 90-99
GTTAT 1698300 0.8922501 11.106644 110-119
GAGAT 2334735 0.8877869 12.189185 210-219
GGTAG 2808770 0.8829281 12.566983 120-129
CGGAG 3481555 0.87899375 73.57612 6
CGTAT 2071280 0.87400615 7.7980857 240-247
AGAAG 2451565 0.87225807 16.015366 140-149
TCCTT 1843640 0.86328906 10.467068 45-49
TATTT 1262785 0.8576957 10.378831 240-247
ACGCT 2521585 0.85457987 15.650899 190-199
ATAGT 1729830 0.8503664 15.812739 120-129
GATGA 2223735 0.84557897 6.699846 60-69
ATCCA 2057255 0.8433914 9.702158 180-189
TTACC 1908850 0.83633906 9.645954 45-49
TCTTC 1782700 0.8347538 46.236233 2
GGTTC 2389790 0.8336301 11.677737 160-169
GTTTC 1848405 0.8335705 5.10233 100-109
GCCTA 2447565 0.82949406 16.282705 190-199
AGTCT 1964740 0.82905 14.763086 8
CATGG 2531750 0.8263517 8.481715 200-209
GATCA 2089685 0.82506263 9.065926 110-119
GTCTG 2343660 0.81753856 14.723482 3
ATATG 1652780 0.81248945 5.933869 50-59
ATATA 1365520 0.8120126 11.1264515 130-139
TGGCA 2483340 0.81055087 5.7844243 100-109
GTCTA 1918030 0.8093401 10.17491 30-34
CTATC 1847215 0.80933446 8.099639 110-119
CCTCC 2678465 0.8090454 10.860624 140-149
GCTTG 2316330 0.80800503 16.876099 90-99
ACATC 1955065 0.80149764 5.8380017 110-119
GGTGC 2966540 0.8004465 11.589285 60-69
TCATC 1822585 0.79854316 10.288711 110-119
ACAAA 1772860 0.7922697 12.849784 130-139
TATAC 1546880 0.78957653 9.229719 130-139
TTATG 1481155 0.7781669 11.318 50-59
CATTA 1520920 0.7763258 12.48505 110-119
TGATG 1894605 0.7699444 11.903927 120-129
GTTCA 1823855 0.76960164 14.139911 160-169
GGTTA 1849775 0.7517261 8.321648 50-59
CCCCC 3075000 0.74599415 13.506515 80-89
CTGAC 2188630 0.74173945 9.488083 220-229
CCAGC 2719320 0.7401893 6.2328796 80-89
GCGGA 2916920 0.7364395 9.222898 220-229
GGGAT 2337165 0.7346806 13.202042 100-109
AACGC 2312175 0.73321307 14.820451 180-189
GACCG 2789105 0.7311598 9.5327835 210-219
CATCC 2068725 0.7279754 6.62279 110-119
TCAAC 1772640 0.72671074 7.648895 110-119
TTGGA 1782695 0.72446555 6.4279795 60-69
ACGCC 2660385 0.7241474 13.17215 180-189
ATAAA 1299020 0.7227881 7.9979453 130-139
ATGCC 2117110 0.7175009 8.417361 50-59
ATCTT 1314075 0.71684843 12.552605 3
GATTA 1456750 0.71612316 5.5976424 50-59
TCCGG 2540405 0.7117377 12.020859 70-79
TGCCA 2097835 0.7109685 7.698865 60-69
CAACG 2235180 0.7087973 15.088545 180-189
TTTAC 1294080 0.70594084 13.073802 45-49
CGCCG 3117055 0.7013986 11.00012 220-229
GGGCA 2777110 0.70114154 13.5352 150-159
TATTG 1333135 0.7004004 6.460889 240-247
GACCA 2193010 0.6954246 6.0720716 170-179
AATAC 1451210 0.6931039 11.645364 130-139
TATAG 1403975 0.6901795 6.3434906 120-129
GAAAT 1498865 0.6894387 7.3188396 110-119
GGATA 1809445 0.6880445 15.235331 35-39
CACAA 1782925 0.6839189 8.259521 130-139
CAAAC 1772370 0.67987007 13.782797 130-139
AAATA 1206315 0.67120606 13.010498 130-139
TTTCT 1145265 0.6677021 9.88551 4
GCCGC 2947000 0.6631329 12.089473 220-229
CACGC 2430940 0.6616933 12.608267 190-199
AGGCA 2137670 0.65285265 6.5929513 80-89
CAGTC 1919720 0.6506043 5.4119883 210-219
CCCGT 2217645 0.64512515 9.946313 140-149
AATAA 1149770 0.6397438 7.5573444 130-139
ACTTC 1459510 0.6394663 30.56147 4
GCGAC 2438710 0.63930434 7.0326734 70-79
TACTT 1162925 0.63439375 12.034937 1
GACGA 2072715 0.6330151 9.752628 220-229
TCCAC 1780235 0.62645704 10.174773 180-189
CCGGC 2782135 0.62603503 9.7027235 70-79
AGATG 1643355 0.6248885 5.873402 120-129
TCAAT 1219705 0.6225761 8.463473 110-119
TAGCT 1466155 0.61866504 12.834353 50-59
AAGCA 1662120 0.61404324 5.9512157 120-129
CACCG 2227815 0.6064034 9.0636015 45-49
CCCTA 1704890 0.59994346 11.745622 110-119
CACGA 1865565 0.59158874 8.773169 180-189
GGCCC 2626530 0.5910209 10.180398 70-79
AATGG 1540555 0.5857986 6.6091685 120-129
ACCTA 1422370 0.5831142 7.2113104 130-139
TTAGC 1376575 0.58086544 10.077963 45-49
CTTTG 1279915 0.5772 7.08297 45-49
CCGTA 1699630 0.5760145 6.67139 230-239
ACGAC 1808345 0.5734437 9.045554 220-229
ACCCG 2104095 0.57272726 8.761642 140-149
TGATT 1082535 0.5687405 6.031248 50-59
CGGTT 1625790 0.56712407 11.569097 160-169
GGGGA 2314625 0.5628055 8.677615 4
CGGCA 2137095 0.56023633 11.220918 70-79
TAATA 939065 0.5584192 8.18401 130-139
GTTCC 1537140 0.55675226 6.738472 140-149
GGTGA 1765805 0.5550753 6.206005 120-129
TCAGG 1692250 0.5523427 5.4147854 150-159
ATTAA 920760 0.5475341 6.353544 110-119
CTGCG 1951935 0.54686785 11.890425 25-29
ATTTC 998765 0.5448419 6.0283475 240-247
TTCAA 1067045 0.54465353 5.420835 200-209
GAAGA 1501495 0.53422654 6.1054225 15-19
CCTAT 1210120 0.5301991 5.0378175 110-119
ACCCC 1875270 0.5300065 9.314562 140-149
CCACG 1944645 0.5293255 7.6577597 180-189
GCTAC 1552355 0.5261022 7.936397 50-59
GGAGG 2155885 0.5242075 5.6133084 6
AAATC 1092315 0.5216942 7.9021716 110-119
GTCCT 1438425 0.5209977 6.3094997 45-49
AAGAT 1125710 0.5177971 5.5413456 15-19
CTGTT 1139355 0.513812 5.7153435 240-247
TATGT 976430 0.51299524 6.741629 140-149
GGGTA 1614225 0.5074266 5.374079 100-109
ACATT 982275 0.5013843 6.1879683 110-119
AGTTT 943165 0.49551854 5.0317616 140-149
GGGTT 1457715 0.48972398 5.814447 100-109
TGACG 1471100 0.48016036 8.592979 220-229
CGCGG 2210175 0.47897425 6.4698367 220-229
GTAAC 1210185 0.47781286 6.7849135 120-129
AGTAT 968660 0.47618315 5.2075515 110-119
TATCC 1077975 0.47230145 5.33649 110-119
TAAAT 785925 0.46735385 8.099776 130-139
CCGGT 1667990 0.46731585 8.952179 160-169
AACAT 974920 0.46562582 5.9724865 110-119
TGGCC 1660365 0.46517956 14.715755 2
CCATT 1059890 0.46437773 5.0671983 110-119
CATAA 955045 0.45613348 6.1614037 110-119
GGTTT 1042465 0.45276386 5.441125 100-109
CAATC 1101165 0.45143315 6.3256454 110-119
ATGCT 1068810 0.45099962 6.9899583 50-59
CCGCG 1966120 0.44241565 6.6861963 220-229
TGCTA 1037420 0.43775418 6.2449408 50-59
ATTCT 789125 0.43048 6.96756 2
TGCGC 1527625 0.4279902 8.875585 25-29
TTGGT 983175 0.42701295 5.17834 120-129
GCCTT 1166350 0.4224521 9.032078 1
GGTCA 1283970 0.419082 5.286706 160-169
GCATG 1280505 0.41795102 8.404296 60-69
GCGCT 1479660 0.41455194 8.110437 25-29
CACTT 928640 0.40687215 7.2682977 3
CTAAC 974335 0.39943796 5.5411224 110-119
ACGAG 1303325 0.3980405 7.703238 180-189
ATGGC 1200815 0.39194056 6.8982105 1
CCGGG 1803910 0.39093122 5.1485686 80-89
CCTAA 950925 0.38984078 7.5785217 110-119
TACCT 888835 0.3894321 5.288165 2
CATGC 1138985 0.38600865 6.9035635 60-69
GGTAA 1002340 0.38114145 7.131878 120-129
AGCTT 900900 0.38014755 7.63544 1
TTTGA 720820 0.37870327 5.95546 45-49
GAGGC 1467390 0.37047434 5.7582693 35-39
TTCTT 619050 0.36091295 19.838747 1
CGGTG 1304970 0.35211346 5.1762247 8
TGGGC 1288985 0.3478003 9.477463 1
TGCTG 980405 0.34199452 13.925888 1
CGAGC 1281405 0.3359185 6.4845247 180-189
ATGTG 825850 0.33561543 5.11267 140-149
AACCT 772460 0.3166774 5.823041 2
CTCTT 657700 0.3079697 9.557695 1
GCGAG 1195135 0.30173764 5.3920827 240-247
CGCTT 811750 0.29401594 5.740075 1
AACTT 571695 0.29181123 10.843128 3
GACTT 688865 0.29067636 6.779002 1
CTCGG 1016390 0.28475896 5.649052 6
TTCCT 599405 0.2806729 6.574172 4
TCACA 683370 0.28015408 5.4602513 5
ATTGT 531615 0.27929905 5.2437363 240-247
GAGGT 843875 0.2652695 7.0841055 7
CCCTT 695350 0.2615092 5.340499 3
TAACT 509775 0.26020533 7.106399 2
GTCTT 563960 0.2543276 11.443532 1
TGTAA 508340 0.24989465 7.5855947 25-29
TTGGC 715830 0.24970287 7.3585534 1
AATCT 482285 0.24617353 7.2488794 2
CGGGG 1171195 0.24444422 8.599583 3
CCTCG 795300 0.23135719 6.091229 5
TGCTT 474690 0.21406974 11.227248 1
TCGGT 570040 0.19884697 6.733196 7
TGCCT 547480 0.19829732 5.1080337 4
TCGCA 469150 0.15899767 5.55288 7
TAGGC 478590 0.1562096 7.1858716 1
TTGCT 270485 0.12197993 5.6547303 4
Produced by FastQC (version 0.10.1)