FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_3_SL248349.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_3_SL248349.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9542262
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 11111840 8.852808 62.934998 110-119
ATACA 10122625 7.647449 38.80026 110-119
CACAG 14789885 7.576209 43.104015 110-119
TCCCT 17352065 7.098641 108.31187 30-34
CAGTA 12550040 6.7795963 40.08619 110-119
AGTAA 9627285 6.494607 49.140076 110-119
GGAGA 15313140 6.2546444 96.1423 35-39
GTAAT 10544735 6.007115 40.65455 110-119
CCCTG 17314510 5.997748 71.07789 30-34
GGTGA 17042610 5.8783526 102.74767 45-49
AATAC 7352610 5.5547566 40.822826 110-119
GTAGT 13131465 5.349075 28.601072 80-89
AGACG 11573885 5.294097 103.286476 40-44
ACAGT 9744095 5.2638097 36.323048 110-119
GTGAC 13095025 5.0582504 85.68002 45-49
CCCCA 10425730 4.7893624 102.60043 60-69
GAGAC 10421540 4.7669945 142.88844 40-44
ACTCC 9765935 4.73104 123.312515 30-34
CTCAG 10873110 4.7035108 35.23428 140-149
ACCAG 8981370 4.600762 96.04303 45-49
TAGTA 8060475 4.5918837 36.525043 80-89
CACTC 9100265 4.4085608 101.058464 25-29
GCACA 8601850 4.4063506 34.659294 110-119
GGCCC 14973145 4.3918257 59.388607 60-69
CGTGT 13276410 4.3306775 23.626072 120-129
GTGTC 13268830 4.3282046 24.014647 120-129
TGACC 9958785 4.3079906 146.11142 45-49
AGTAG 8858400 4.273066 29.724838 80-89
CTCCC 10888840 4.224099 70.82999 30-34
ATGCA 7569555 4.0891128 98.77039 25-29
GACCA 7855405 4.023979 108.634 45-49
CCCAG 9785730 4.014117 87.728935 60-69
TGAGG 11070515 3.8184521 70.1308 35-39
CCACT 7880615 3.8177094 28.382488 240-247
TACAC 6234820 3.771873 28.334421 110-119
AGGAG 9187425 3.752599 96.58054 35-39
ATGGT 9131015 3.7195 25.81274 190-199
CAGGG 11302265 3.6966934 36.21321 50-59
ACGGT 9529450 3.6809657 97.348404 40-44
GACGG 11224070 3.6711183 108.925575 40-44
AGATA 5438785 3.6690273 19.96538 150-159
CTGAG 9441455 3.646976 106.305176 35-39
TGCAC 8280565 3.582023 118.13756 25-29
CCCTT 8730695 3.571682 34.439613 20-24
GAGGA 8679125 3.544984 126.52892 35-39
TGTCC 9694315 3.541333 20.763288 120-129
GGGTT 11895760 3.4649181 42.587387 50-59
GCCCC 10469990 3.439163 73.423874 60-69
CCTGA 7920920 3.4264467 86.497284 30-34
TGGTG 11721890 3.414274 17.913393 190-199
GGATG 9711385 3.34966 71.31087 20-24
TCTTG 8672500 3.3409157 18.19259 170-179
GCACT 7684005 3.3239617 91.46683 25-29
CCAGG 9059565 3.3184075 41.13201 50-59
CCGTG 10714405 3.314146 23.787645 120-129
AGGCT 8494835 3.2813225 24.295181 30-34
CTCTC 7989315 3.2683878 13.722084 130-139
CTTGG 10017085 3.2675073 19.605078 20-24
CTTCA 6382555 3.2606857 17.093788 200-209
CAGAT 6030905 3.257926 18.264933 150-159
TCAGG 8400450 3.2448642 21.174688 140-149
TCGCA 7361845 3.184601 24.681662 100-109
CCTGG 10231885 3.1648943 29.80429 50-59
TGTCT 8159490 3.143288 19.685354 180-189
TCTCA 6121485 3.1273117 22.169739 130-139
CGCAC 7569370 3.1049635 25.269096 100-109
CGGAT 7935175 3.0651407 81.08655 20-24
ACAGA 4790175 3.064283 14.78664 140-149
GGTTG 10511540 3.0617313 74.396 15-19
TTCCC 7435445 3.0418024 50.48001 50-59
ACCAC 5225605 2.9977689 20.359097 230-239
GATGC 7662490 2.9598105 97.89912 25-29
GGTTC 8995610 2.9343085 48.13168 50-59
TGGCC 9421190 2.914133 43.670555 60-69
AGGGT 8407370 2.8998778 50.685787 50-59
GACTC 6685535 2.8920417 20.01583 190-199
GTTGG 9795055 2.8530383 53.673306 15-19
GCCCT 8204240 2.8419497 18.578455 20-24
TCAAA 3745095 2.8293478 58.938557 70-79
TCTCT 6515025 2.8106816 14.984288 180-189
CGGCC 9372400 2.7490516 13.083291 120-129
CGGTG 9942570 2.7461727 68.36671 40-44
TTCTT 6034000 2.7451904 20.477024 170-179
GCCGT 8838705 2.7339606 22.459898 120-129
AGTCA 5045465 2.7255864 32.49067 70-79
CCAGT 6286080 2.7192447 36.76377 60-69
GATGG 7880650 2.718201 19.242908 15-19
TCACA 4424950 2.6769578 11.945026 140-149
GTTCC 7308190 2.6696815 54.57986 50-59
AGATG 5485030 2.6458383 17.208662 180-189
AGGGA 6329310 2.5852034 9.41566 150-159
TCCAT 5047755 2.5787702 17.900654 70-79
GCGGA 7794930 2.5495303 89.050766 20-24
TGGAG 7200630 2.4836478 16.512634 25-29
TCCTC 6057385 2.4780452 16.994122 130-139
TGTTC 6429790 2.476954 13.043111 140-149
GGCTG 8953650 2.4730296 12.529112 30-34
ACAGC 4815060 2.466544 11.3086815 120-129
TACCA 4060995 2.4567764 16.920715 230-239
ACTCT 4793530 2.4488928 13.743438 200-209
GGCGG 10434585 2.440385 42.988407 20-24
GAGAT 4971305 2.3980305 16.866821 180-189
TTGGG 8159210 2.3765602 80.520966 15-19
GTTCT 6161565 2.3736253 18.000229 160-169
CAGCT 5471105 2.3667011 12.444965 150-159
CACGG 6426675 2.3540123 9.887147 120-129
CTGGC 7555355 2.3369987 35.14444 60-69
CTGGA 6046730 2.3356862 14.722603 180-189
TGTAG 5707570 2.3249667 8.263444 210-219
AATAA 2446585 2.308204 9.689136 110-119
ACTAC 3754985 2.2716496 13.068415 230-239
CATTT 4212940 2.269714 17.796595 150-159
CTACT 4414375 2.2551925 7.672715 230-239
TCATT 4178165 2.2509794 16.399239 150-159
CAGAG 4900135 2.2414072 9.77379 140-149
CACTA 3701585 2.2393444 15.646 240-247
GTGAA 4609500 2.223505 18.214811 190-199
TTCAT 4122325 2.2208955 16.240732 140-149
TTCAC 4293310 2.1933432 18.710386 200-209
TCAGC 5066410 2.1916375 14.805768 140-149
GATAC 4045765 2.1855433 14.907092 150-159
TTGTC 5549465 2.1378255 13.332742 170-179
ATTTG 4421380 2.127009 15.701541 150-159
TGGGG 8578420 2.1157389 53.241917 15-19
TAGTC 4600315 2.0985894 26.820772 70-79
ATATA 2627905 2.0936532 11.039433 110-119
CTCGC 6038240 2.0916474 16.62865 100-109
TTGGA 5125280 2.0877721 16.03546 170-179
ATGTA 3647660 2.0779955 7.9378476 70-79
TCTGC 5669685 2.0711358 12.035627 210-219
AGCTC 4764965 2.0612378 12.74529 140-149
TACAG 3805315 2.0556507 13.987832 160-169
CTCTG 5626275 2.0552778 14.599134 180-189
ACACA 2868130 2.054711 14.220325 110-119
GAACT 3787490 2.0460217 11.137419 160-169
GAATC 3772030 2.0376701 14.420005 190-199
GCTGT 6243075 2.03645 10.233625 140-149
GTCTC 5553920 2.0288467 11.833207 180-189
GTCCT 5543620 2.0250843 14.199816 130-139
GTCAA 3740235 2.0204945 41.358967 70-79
TCTCG 5517015 2.0153651 15.998416 100-109
CAGGC 5496985 2.013478 15.432364 140-149
GATAT 3519505 2.0049884 8.623811 180-189
GTTCA 4383620 1.999737 15.2739 140-149
TGAAT 3504460 1.9964176 13.872131 190-199
ATCTC 3902135 1.9935018 7.7642813 100-109
AATCG 3685890 1.9911368 13.521159 190-199
ACGGC 5433565 1.9902481 14.891983 120-129
AATAT 2497760 1.9899665 13.659343 110-119
AACCA 2770760 1.9849559 15.481281 70-79
TTGCA 4319370 1.9704269 14.587897 150-159
ACACG 3831940 1.9629347 13.724015 120-129
TGGAA 4048235 1.9527651 9.529722 170-179
CCTTC 4764565 1.9491588 14.129106 200-209
TACTA 3053885 1.9483069 10.323078 230-239
ATATG 3415680 1.9458416 8.8399105 180-189
TTTGC 5031390 1.9382468 12.252924 150-159
GAGTC 4974070 1.9213473 12.3293915 210-219
CAGCC 4635855 1.9016327 10.23454 120-129
GGGGC 8063710 1.8858978 48.689693 15-19
CATAG 3490305 1.8854808 8.472585 210-219
TCGGC 6095615 1.8854766 8.059458 130-139
AGTAT 3238505 1.8449086 12.17739 220-229
GGGCG 7842030 1.8340523 59.833256 20-24
GACGT 4745790 1.833169 9.987006 170-179
AGAAC 2814390 1.8003701 11.822352 160-169
CCAGA 3510310 1.7981778 27.268509 60-69
GTTGT 5223350 1.796784 7.1507664 210-219
CTGTT 4615805 1.7781508 12.799693 140-149
CGTAG 4602220 1.7777117 9.873712 210-219
GCAGA 3831150 1.7524351 14.909558 150-159
ATTGT 3588050 1.726116 7.7241344 170-179
AGTCT 3775290 1.7222265 13.12591 210-219
CACCA 3001745 1.7220088 13.578706 230-239
CTCCA 3531055 1.7105954 7.888769 150-159
GGTCA 4403980 1.701137 7.9398475 130-139
TGACT 3727070 1.7002293 11.928369 190-199
TGCAG 4401430 1.7001522 13.669887 150-159
GTCAC 3915665 1.693846 8.905625 130-139
TCCCA 3484105 1.6878507 10.79252 240-247
ATAGT 2957680 1.6849283 7.3318424 220-229
AACTG 3109375 1.6797005 11.0609045 160-169
GAGAA 2929540 1.6734108 10.828378 160-169
TGGTT 4820365 1.6581609 18.330418 10-14
CCTTG 4510080 1.6475317 22.349422 20-24
AATAG 2430130 1.6393759 10.016603 240-247
TCAGA 3026215 1.6347771 10.322996 130-139
CAAAA 1824480 1.63223 21.643097 70-79
TAGTT 3391770 1.6316909 10.126245 220-229
ACTGA 3006210 1.6239702 9.107242 180-189
TTGTA 3370565 1.6214895 9.2825575 210-219
GCTCC 4651095 1.61114 6.3753443 150-159
TGTGC 4938975 1.6110611 8.229586 220-229
GTGCT 4913675 1.6028084 8.383493 220-229
GAACC 3127480 1.6020708 12.662057 70-79
ATAGA 2372315 1.6003737 10.37881 240-247
TGCTC 4364480 1.5943444 5.284257 150-159
CAAAG 2489975 1.5928411 29.73056 70-79
TTCAG 3482475 1.5886489 9.496488 150-159
GTCTG 4866440 1.5874008 9.054724 210-219
TCTGG 4860695 1.5855268 11.959231 180-189
CTGTG 4842780 1.5796831 5.285799 210-219
GCTCA 3641145 1.5750935 11.539814 140-149
CTGGT 4822385 1.5730301 7.1208167 160-169
TTGGT 4572495 1.5728958 23.448635 25-29
GTCCC 4535740 1.571181 20.182682 50-59
GGCCG 5984115 1.5673196 13.362038 120-129
GGACG 4762625 1.557738 7.4511294 170-179
AAAAA 1384445 1.5467081 7.859965 8
CCCAC 3348495 1.5382285 10.453199 240-247
CCACC 3326040 1.5279132 9.9589815 230-239
ACCAT 2513650 1.5206804 11.785243 45-49
GCTCT 4034465 1.4737899 10.204918 130-139
CCTCG 4242120 1.4694707 9.717744 130-139
TATAC 2283835 1.457033 7.1095667 110-119
CGACT 3357580 1.4524285 8.972273 190-199
CAGAC 2820285 1.4447082 16.282585 60-69
TGATA 2528235 1.4402826 8.08328 180-189
CACTG 3308195 1.4310653 7.4608274 230-239
CATGT 3136835 1.4309735 7.8373666 70-79
TCGAC 3288970 1.422749 9.615553 190-199
GCTGA 3678485 1.4208981 20.86611 30-34
AATTG 2486755 1.4166523 9.559664 170-179
TGGAC 3655865 1.4121608 10.545717 170-179
AAATA 1495700 1.4111018 5.2125554 9
GGGAG 4797985 1.4013044 6.750142 150-159
CTCTT 3240375 1.3979474 8.276469 200-209
GAGGG 4768050 1.3925614 8.545975 200-209
GCGTA 3540975 1.3677816 10.33514 210-219
CCATC 2804795 1.3587636 10.8762045 240-247
CCTCA 2794865 1.353953 7.369453 130-139
CTGAT 2950100 1.3457881 7.564165 180-189
ACGGA 2935285 1.3426505 9.477676 200-209
CTCGA 3095840 1.3392044 8.642754 190-199
ACTTC 2600770 1.3286674 6.665674 220-229
TATGG 3253735 1.3254021 6.362659 180-189
TCCAC 2727945 1.3215343 10.004276 240-247
GAATT 2317320 1.3201288 10.561708 170-179
CTGCT 3612180 1.3195293 5.807458 90-99
GTGGA 3806945 1.313095 21.081383 25-29
CTTGA 2871685 1.3100165 8.570538 200-209
GTAGG 3791760 1.3078573 5.150267 160-169
AGATC 2411040 1.3024564 7.175353 130-139
CCATG 3006585 1.3005943 7.9585676 70-79
ACCGT 3006200 1.3004278 19.129208 45-49
ACGTG 3333185 1.2875181 9.605883 170-179
GTGTT 3705350 1.2746061 7.611877 160-169
AGCCG 3464975 1.2691779 9.148262 120-129
ACTAT 1988300 1.2684886 6.238376 230-239
GGAGT 3652770 1.2599168 7.0664773 210-219
GGAGG 4305395 1.257438 15.784239 30-34
GGCTC 4044770 1.2511156 8.491453 130-139
ACTCG 2890930 1.2505641 8.813042 190-199
GTCCA 2883650 1.247415 16.694778 70-79
AAAGT 1848350 1.2469047 21.867764 70-79
ATAAC 1644600 1.242464 7.8701363 80-89
AAGTA 1835355 1.2381382 16.102808 70-79
ATCGG 3205060 1.238027 7.958532 190-199
GGAAT 2552335 1.2311811 8.843505 170-179
ACTGG 3170810 1.2247971 7.3945827 160-169
GATCT 2676195 1.220837 5.6602173 130-139
GTGGT 4185420 1.2191014 10.772862 50-59
TTGAG 2969855 1.2097642 8.262877 200-209
AGCTT 2642045 1.2052584 6.4049225 150-159
TTGGC 3689960 1.2036405 8.110029 60-69
TGGTC 3670655 1.1973435 12.252612 50-59
GCATA 2203300 1.1902341 7.986966 210-219
TTCCA 2319065 1.1847516 7.535938 230-239
ATACC 1954800 1.1825935 7.700092 70-79
TGCCC 3402240 1.1785365 6.8666244 60-69
ATAAA 1239655 1.169539 5.656285 7
ACTGT 2526095 1.1523638 5.6300335 230-239
AGAGC 2513960 1.1499292 7.334905 140-149
TCACG 2649355 1.1460631 9.5660515 200-209
ATCCA 1879955 1.1373147 16.03003 240-247
TCTAC 2219200 1.1337332 7.4549875 180-189
CCATA 1865525 1.1285849 10.622579 70-79
TATCA 1761725 1.1239392 6.743224 70-79
GTCTA 2455140 1.1199954 7.5251846 180-189
AGTTC 2439700 1.1129519 5.4706545 160-169
TCTGT 2888750 1.1128358 6.3977857 210-219
AGGTT 2717280 1.1068784 23.506714 10-14
GCTTC 3018375 1.1026123 5.666985 150-159
AAAAG 1379185 1.1017686 14.363872 70-79
CAATA 1454660 1.0989678 6.6748595 100-109
TACTG 2404385 1.0968416 6.684452 180-189
GAGCT 2828790 1.0926843 6.5450344 140-149
TATGT 2270010 1.0920417 7.7596936 220-229
CTACC 2246560 1.0883306 8.937507 230-239
CGGCT 3507665 1.08498 7.9294767 130-139
CTCGG 3479795 1.0763593 8.424686 130-139
CTGCG 3406705 1.0537513 6.948096 210-219
CTGCC 3004995 1.0409307 5.7823086 200-209
AACTT 1628305 1.0388204 6.053718 200-209
ATACG 1922600 1.0385984 5.492399 120-129
ATATC 1626430 1.0376241 7.2872767 70-79
GAAGA 1813905 1.0361382 17.525328 35-39
TAGAT 1818790 1.0361265 7.9615126 240-247
AGAGA 1812450 1.035307 13.111998 35-39
GTCCG 3335985 1.0318764 5.020856 130-139
GGGGT 4172345 1.0290465 7.8732123 10-14
ATGGG 2974225 1.0258725 30.164408 15-19
CATGG 2649320 1.0233598 5.3520813 190-199
GCGGT 3695240 1.0206382 5.902751 130-139
CTGAA 1875950 1.0133979 14.333326 35-39
AGAAA 1264560 1.0101999 5.1727595 7
TGCGT 3081945 1.0053102 8.279671 210-219
ACCCA 1731135 0.9930989 13.204078 240-247
TGCTA 2158730 0.9847779 5.087977 220-229
CTTCT 2279435 0.9833832 5.4466634 210-219
TATAA 1232945 0.9822877 6.4867806 240-247
AAAAT 1038880 0.98011994 5.186826 8
ACTAA 1296220 0.9792694 12.99254 240-247
CGAAC 1908245 0.9775102 13.310802 70-79
TAGGC 2529010 0.97688746 5.8722787 160-169
TCCCC 2496375 0.96841675 5.172367 80-89
CAGCG 2643465 0.9682688 6.441715 160-169
TGTAA 1699015 0.9678933 6.9601827 230-239
AGCTG 2496570 0.9643567 5.1516366 90-99
CGGAG 2930460 0.95848155 6.6090302 210-219
CCCAA 1650045 0.9465801 10.152997 240-247
CGTGG 3376485 0.93259704 7.474593 50-59
ACAAT 1227515 0.92736405 7.5859113 100-109
CCATT 1811740 0.925572 5.6538644 50-59
GAGTT 2260320 0.9207367 5.245422 160-169
TGCAT 1977370 0.90204436 6.0897484 210-219
AAGAG 1576995 0.9008104 13.045107 35-39
TCGAA 1667100 0.90057606 16.825449 70-79
CCAAT 1463925 0.8856293 10.992078 240-247
TGAAG 1813740 0.87490183 9.484443 35-39
ACGTC 1996250 0.8635416 11.445776 70-79
TTCTG 2237920 0.862116 5.3832273 210-219
CATCA 1420080 0.8591045 5.552157 230-239
TGAAC 1584400 0.8559011 6.765116 190-199
GGTGG 3457830 0.852822 20.826017 25-29
CGGTC 2754870 0.8521278 5.750895 130-139
CCAGC 2071350 0.84967 5.8026156 80-89
CTTCC 2055480 0.8408863 5.3306313 230-239
GAGGC 2550665 0.83426 13.458505 30-34
ATCAA 1104220 0.8342171 12.803988 70-79
GTATG 2039940 0.83096534 5.404839 220-229
AGCGT 2142025 0.8274057 6.63138 160-169
AACCG 1582920 0.8108605 5.9909263 190-199
TAACC 1324195 0.801097 6.2770205 80-89
CGTCC 2271780 0.786945 9.35223 70-79
GGGCC 3000325 0.7858252 15.912275 15-19
CTAAT 1214180 0.7746183 9.939485 240-247
AAAGG 1341825 0.7664767 9.173194 10-14
AAAGA 959315 0.7663534 5.834517 8
AATCC 1257335 0.7606487 9.962989 240-247
TGAGA 1569985 0.75732064 5.7037 240-247
GACCG 2053495 0.75217 17.582718 45-49
GGTCC 2421140 0.74889946 12.427391 50-59
ATGAT 1311350 0.7470486 6.861153 240-247
AGCCA 1457185 0.746452 6.0035105 80-89
TGGGC 2653530 0.7329143 17.251938 15-19
CATAC 1190435 0.7201763 9.214786 70-79
GTCGA 1856345 0.71705526 11.878751 70-79
GTAGC 1853740 0.7160491 5.630021 80-89
AAGGT 1474020 0.7110296 10.144828 10-14
AAAGC 1048820 0.67093194 9.681503 70-79
CAATC 1095545 0.6627708 9.52965 240-247
CATTG 1439995 0.6569025 5.1362667 50-59
CCCAT 1355990 0.6569 5.0001926 240-247
TATGA 1151830 0.6561734 5.630636 240-247
TAGGT 1601820 0.65249807 5.452578 10-14
AGGAT 1325085 0.63918716 5.7999043 10-14
GGAAG 1560755 0.6374896 5.8686213 30-34
GAGGT 1838890 0.6342716 6.9740486 10-14
ATAGG 1286065 0.6203649 5.19825 10-14
AGAAG 1081035 0.61750835 5.5020466 9
CCGTA 1421070 0.61472917 5.3205895 80-89
CGATG 1538000 0.59408736 13.2321205 15-19
AATGG 1227550 0.59213877 6.8222036 10-14
CATCC 1219695 0.5908729 7.448837 240-247
GGGTC 2016805 0.5570486 5.697851 70-79
AAATG 824320 0.55608976 5.0996275 9
TAAAA 589190 0.5558649 6.148861 8
GAAGG 1319365 0.53889394 5.568527 10-14
AAGAA 669620 0.53492916 5.400063 8
AAGGG 1275830 0.52111214 6.333963 10-14
AAGCA 804800 0.51483196 6.4978604 70-79
GAAAA 640950 0.512026 6.4160924 8
TGATC 1111190 0.506907 5.301269 240-247
GGTCG 1776285 0.49061617 5.809312 70-79
GATCC 1131620 0.48951837 5.403031 240-247
ATAAG 679875 0.45864654 5.6845846 9
ACGAT 838355 0.4528837 6.108772 10-14
CGGTT 1344940 0.43871054 8.177034 10-14
TAAGG 887925 0.42831233 5.874619 10-14
TAAAG 593770 0.40055975 5.594591 9
AAGCT 739235 0.39933854 7.9912105 30-34
GAAAG 675900 0.3860873 5.027197 9
TGGGT 1319985 0.38447648 5.1081953 10-14
Produced by FastQC (version 0.10.1)