FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_6_SL248352.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_6_SL248352.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11572873
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 13462845 8.8166485 62.949757 110-119
ATACA 12248800 7.60571 38.803387 110-119
CACAG 17875130 7.537249 43.042027 110-119
TCCCT 21148375 7.141542 109.72603 30-34
CAGTA 15309915 6.8085837 40.858604 60-69
AGTAA 11633300 6.45165 48.915466 110-119
GGAGA 18622570 6.263924 96.181366 35-39
CCCTG 21250835 6.077042 71.944954 30-34
GTAAT 12713190 5.9629197 40.62531 110-119
GGTGA 20598340 5.859703 102.02018 45-49
AATAC 8887140 5.518338 40.943047 110-119
GTAGT 15690935 5.2709756 26.239368 80-89
ACAGT 11769415 5.234062 36.34126 110-119
AGACG 13834780 5.210237 102.96664 40-44
GTGAC 15806605 5.034547 85.018745 45-49
ACTCC 12000010 4.791374 124.52235 30-34
GAGAC 12701840 4.7835674 142.8082 40-44
CCCCA 12577635 4.761646 102.37916 60-69
ACCAG 11125245 4.6910844 97.581535 45-49
CTCAG 13088040 4.667397 35.331547 140-149
CACTC 11168400 4.4593287 102.12782 25-29
TAGTA 9503845 4.4576273 33.357986 80-89
GCACA 10412145 4.3903975 34.84964 110-119
GGCCC 18111785 4.3861017 59.972668 60-69
CGTGT 16025275 4.316818 23.690346 120-129
GTGTC 16007245 4.3119617 24.124208 120-129
TGACC 12032670 4.291036 145.33224 45-49
CTCCC 13292245 4.2559147 71.186584 30-34
AGTAG 10612630 4.2153015 28.754108 70-79
ATGCA 9310800 4.1406736 99.14131 25-29
GACCA 9668420 4.0767975 109.43889 45-49
CCCAG 11831940 4.0006976 87.38991 60-69
CCACT 9608620 3.836538 28.348793 240-247
TGAGG 13465570 3.8306122 70.03111 35-39
AGGAG 11193690 3.765132 96.66621 35-39
CAGGG 13925430 3.7560523 36.976364 50-59
TACAC 7523950 3.7463496 28.374096 110-119
ATGGT 11019375 3.7016823 25.770584 190-199
ACGGT 11570875 3.6854281 97.54736 40-44
CTGAG 11529205 3.6721566 106.67483 35-39
GACGG 13610455 3.6710954 109.36898 40-44
AGATA 6591885 3.6557586 19.85713 150-159
TGCAC 10161810 3.6238582 119.63132 25-29
GAGGA 10572870 3.5563116 126.75546 35-39
GGGTT 14672525 3.530082 43.76903 50-59
TGTCC 11675090 3.5212502 20.824673 120-129
CCTGA 9723985 3.467723 87.40727 30-34
CCCTT 10241710 3.4584975 32.72575 20-24
GCCCC 12652395 3.4305885 73.383 60-69
CCAGG 11203355 3.3833697 41.999073 50-59
TGGTG 14026805 3.3747272 17.922152 190-199
GCACT 9446580 3.368796 92.54485 25-29
GGATG 11826430 3.364318 72.27113 20-24
TCTTG 10567955 3.3616152 18.424667 170-179
CTTCA 7781470 3.27688 17.008457 200-209
CTCTC 9688575 3.2717109 13.791309 100-109
CCGTG 12757730 3.2584517 23.794018 120-129
CAGAT 7266065 3.2313445 18.03868 150-159
TCAGG 10131585 3.2270017 21.157352 140-149
AGGCT 10113540 3.221254 22.956324 30-34
CTTGG 11945470 3.2178185 18.463825 20-24
CCTGG 12573860 3.2114894 30.427002 50-59
TCGCA 8907525 3.176561 24.806747 100-109
TGTCT 9879505 3.1426227 19.842897 180-189
TTCCC 9215740 3.1120405 51.734283 50-59
TCTCA 7386235 3.1104414 22.02911 130-139
CGGAT 9765365 3.1103568 82.19612 20-24
CGCAC 9151400 3.0943346 25.438547 100-109
GGTTG 12803240 3.0803483 75.22934 15-19
ACAGA 5774200 3.0362628 14.709867 140-149
ACCAC 6387555 3.0156202 20.388468 230-239
GGTTC 11141090 3.001138 49.452106 50-59
GATGC 9414955 2.9987485 98.9075 25-29
AGGGT 10438865 2.9695916 51.9514 50-59
TGGCC 11523060 2.9431047 44.093716 60-69
GTTGG 11958860 2.8771975 54.074974 15-19
GACTC 8028040 2.862923 20.09318 190-199
TCAAA 4598415 2.8553176 59.474228 70-79
TCTCT 7942470 2.8287268 15.082281 180-189
AGTCA 6271085 2.7888598 33.11381 70-79
GCCCT 9750415 2.7882993 17.77793 20-24
CCAGT 7816375 2.7874398 37.753307 60-69
TTCTT 7414160 2.7849522 20.952013 170-179
CGGTG 12060800 2.751285 68.59056 40-44
GTTCC 9038175 2.725947 56.068836 50-59
CGGCC 11254160 2.7254019 13.026997 120-129
GCCGT 10651740 2.720561 22.48453 120-129
GATGG 9452190 2.6889076 18.398647 15-19
TCACA 5333995 2.65592 11.86692 140-149
AGATG 6672015 2.6501021 17.235744 180-189
GCGGA 9584660 2.5852332 90.273315 20-24
AGGGA 7646965 2.5721483 9.522871 150-159
TCCAT 5940310 2.5015433 16.293362 70-79
TGTTC 7812915 2.4852505 13.024352 140-149
TACCA 4970930 2.4751415 17.017538 230-239
ACAGC 5845350 2.4647574 11.2105875 120-129
GGCGG 12756250 2.4642444 43.30832 20-24
TCCTC 7287555 2.4609165 16.999535 130-139
TGGAG 8637025 2.4570138 15.58142 25-29
GGCTG 10715575 2.444415 11.81827 30-34
ACTCT 5744145 2.4189355 13.495695 200-209
TTGGG 10003685 2.4067998 81.68562 15-19
GTTCT 7538590 2.397989 18.288422 160-169
GAGAT 6034780 2.3969948 16.778254 180-189
CTGGC 9356605 2.3897707 35.987354 60-69
CAGCT 6628015 2.3636522 12.519401 150-159
CACGG 7748325 2.339964 9.920015 120-129
AATAA 3006680 2.3281846 9.759887 110-119
CTGGA 7294520 2.323371 14.650868 180-189
ACTAC 4622490 2.3016453 13.249494 230-239
TGTAG 6817650 2.2902184 8.374397 210-219
CATTT 5121080 2.2744737 17.79491 150-159
TCATT 5112360 2.2706008 16.598803 150-159
CTACT 5386980 2.2685287 7.776649 230-239
CACTA 4523695 2.2524529 15.713451 240-247
TTCAT 5053380 2.2444053 16.346315 140-149
CAGAG 5939065 2.2366772 9.752581 140-149
GTGAA 5586180 2.2188122 18.02303 190-199
TTCAC 5240365 2.2067873 18.682621 200-209
GATAC 4949825 2.2012727 14.963592 150-159
TCAGC 6146350 2.1918833 14.97168 140-149
TGGGG 10539235 2.1472876 54.017597 15-19
TAGTC 5695820 2.142283 27.481077 70-79
TTGTC 6702555 2.1320508 13.390264 170-179
ATTTG 5356730 2.1249108 15.597479 150-159
ATATA 3223230 2.110853 11.010289 110-119
TTGGA 6237975 2.0954912 16.165243 170-179
AATAT 3189410 2.0887048 13.62853 110-119
CTCGC 7296010 2.0864196 16.741709 100-109
TCTGC 6878865 2.074691 12.052891 210-219
GTCAA 4634025 2.0608308 42.22656 70-79
TACAG 4628815 2.058514 14.11148 160-169
ACACA 3485795 2.052238 14.107425 110-119
AGCTC 5750645 2.0507686 12.889202 140-149
GAATC 4602945 2.0470092 14.224057 190-199
CTCTG 6781320 2.0452712 14.557747 180-189
GTCTC 6756500 2.0377853 11.974851 180-189
GCTGT 7540585 2.0312498 10.186684 140-149
GAACT 4567010 2.0310283 11.204209 160-169
ATGTA 4317195 2.0249116 7.3306518 150-159
GTCCT 6700870 2.021007 14.305964 130-139
TCTCG 6671735 2.01222 16.100863 100-109
GTTCA 5347325 2.0112088 15.240733 140-149
TGAAT 4274970 2.0051067 13.722152 190-199
CAGGC 6632795 2.0030782 15.478319 140-149
AACCA 3388565 1.9949944 15.790431 70-79
AATCG 4460535 1.9836774 13.310319 190-199
GATAT 4225595 1.9819481 8.618274 180-189
ATCTC 4693910 1.9766679 7.993469 100-109
TACTA 3745020 1.9666947 10.368572 230-239
TTGCA 5224095 1.9648603 14.330295 150-159
ACGGC 6493775 1.9610947 14.821693 120-129
CCTTC 5780015 1.951839 13.990997 200-209
ATATG 4144510 1.9439166 8.92045 180-189
ACACG 4603840 1.9412607 13.789711 120-129
TGGAA 4862260 1.9312737 9.474441 170-179
GAGTC 6057915 1.9295008 12.371842 210-219
GGGGC 9902035 1.9128689 49.32164 15-19
TTTGC 6003500 1.9096842 12.150491 150-159
CAGCC 5639080 1.9067247 10.19621 120-129
CATAG 4263025 1.895841 8.632433 210-219
TCGGC 7416395 1.8942217 8.044875 130-139
AGTAT 3975380 1.8645889 12.23243 220-229
GGGCG 9629705 1.8602604 60.53521 20-24
CTGTT 5635290 1.7925588 12.79191 140-149
AGAAC 3407615 1.7918351 11.7557535 160-169
GTTGT 6298305 1.789379 7.2357354 210-219
GACGT 5547200 1.7668333 10.051151 170-179
CGTAG 5522675 1.7590219 9.800997 210-219
GCAGA 4640305 1.7475586 14.655157 150-159
AGTCT 4608965 1.7335005 13.2015295 210-219
CACCA 3656620 1.7263222 13.620006 230-239
ATTGT 4314730 1.7115697 7.69227 170-179
CCAGA 4048550 1.7071164 25.487648 60-69
GTCAC 4778710 1.7041616 8.9771385 130-139
CTCCA 4266880 1.7036836 7.8372636 150-159
TGCAG 5346930 1.7030455 13.510213 150-159
ATAGT 3605400 1.6910554 7.3084116 220-229
GGTCA 5292340 1.6856582 7.933627 130-139
AACTG 3780595 1.681296 11.078279 160-169
TCCCA 4208800 1.6804934 10.746343 240-247
TGGTT 5880610 1.67071 18.444546 10-14
TGACT 4440215 1.6700311 11.931936 190-199
GAGAA 3540035 1.6625577 10.793407 160-169
ACTGA 3663795 1.629353 9.105961 180-189
CAAAA 2215185 1.6263715 21.039314 70-79
AATAG 2930190 1.6250386 10.01564 240-247
TCAGA 3651935 1.6240786 10.423869 130-139
CAAAG 3078070 1.6185496 30.539623 70-79
AAAAA 1767500 1.6182777 8.507514 8
TTGTA 4057420 1.6094999 9.379932 210-219
TAGTT 4055700 1.6088175 10.080529 220-229
GTCTG 5937420 1.5993962 9.101531 210-219
GCTCC 5592740 1.5993403 6.2482147 150-159
TTCAG 4249060 1.5981349 9.601001 150-159
GTGCT 5931840 1.5978931 8.441072 220-229
GAACC 3787135 1.5968878 12.965552 70-79
TCTGG 5914770 1.5932949 11.925131 180-189
ATAGA 2869225 1.5912282 10.334945 240-247
TGTGC 5905570 1.5908165 8.102762 220-229
TGCTC 5267960 1.5888362 5.15343 150-159
CTGGT 5862850 1.579309 7.249789 160-169
CCTTG 5235315 1.5789903 21.022568 20-24
GCTCA 4382350 1.5628135 11.624301 140-149
CTGTG 5782340 1.5576216 5.355142 210-219
CCCAC 4110330 1.5560901 10.763036 240-247
CCACC 4104535 1.5538964 9.989359 230-239
GGCCG 7177895 1.5525163 13.407094 120-129
GGACG 5742960 1.5490264 7.518599 170-179
TTGGT 5422520 1.5405645 22.296316 25-29
GTCCC 5311690 1.5189692 18.800913 50-59
ACCAT 2990235 1.4889075 10.967286 45-49
GCTCT 4925395 1.4855173 10.196566 130-139
CCTCG 5112260 1.4619387 9.6849165 130-139
TATAC 2779420 1.4596103 6.971811 110-119
CGACT 4058540 1.447338 9.002248 190-199
TGATA 3076185 1.4428358 8.130111 180-189
CACTG 4022320 1.4344213 7.4193306 230-239
AATTG 3029200 1.4207982 9.53947 170-179
TCGAC 3960685 1.4124415 9.695027 190-199
TGGAC 4422850 1.4087178 10.575352 170-179
CTCTT 3930170 1.3997381 8.312334 200-209
GGGAG 5776500 1.391584 6.835775 150-159
GAGGG 5757800 1.3870791 8.566263 200-209
CATGT 3682020 1.3848627 7.020237 70-79
GCTGA 4333985 1.3804135 19.562513 30-34
CAGAC 3249620 1.3702387 14.822302 60-69
GCGTA 4286210 1.3651966 10.286602 210-219
CCATC 3388905 1.353125 10.756515 240-247
ACGGA 3582590 1.3492186 9.539515 200-209
CTGAT 3567415 1.3417581 7.571962 180-189
ACTTC 3174970 1.3370221 6.640145 210-219
CCTCA 3344730 1.3354867 7.3547716 130-139
CTGCT 4423135 1.334034 6.0021954 90-99
GAATT 2833410 1.3289659 10.536775 170-179
CTCGA 3716805 1.32547 8.671998 190-199
TATGG 3917535 1.3159975 6.3364415 180-189
TCCAC 3283290 1.3109549 9.85009 240-247
CTTGA 3476485 1.3075578 8.575465 200-209
GTAGG 4581390 1.3032889 5.057774 160-169
ACGTG 4021615 1.2809206 9.683067 170-179
AGATC 2879715 1.280659 7.121492 130-139
AAAGT 2307860 1.2799039 22.49141 70-79
ACTAT 2430350 1.2762966 6.281391 230-239
AGCCG 4215900 1.2731854 9.003939 120-129
AAGTA 2293415 1.2718929 16.714664 70-79
GTGTT 4463910 1.2682186 7.5869775 160-169
GGAGT 4451875 1.266445 7.1214924 210-219
GTGGA 4428225 1.2597172 19.744463 25-29
CCATG 3511145 1.2521285 7.11085 70-79
GGCTC 4889025 1.2487057 8.497904 130-139
ACTCG 3494405 1.2461587 8.946626 190-199
ATCGG 3912445 1.2461492 7.799194 190-199
ATAAC 2002230 1.243255 7.8057837 80-89
GGAAT 3125345 1.2413766 8.813724 170-179
ACCGT 3452390 1.2311755 17.58721 45-49
GGAGG 5100295 1.2286832 14.977996 30-34
ACTGG 3843630 1.224231 7.4489794 160-169
ATAAA 1573915 1.218741 5.827077 7
TTGAG 3600620 1.209538 8.296653 200-209
TCACG 3386175 1.2075622 9.636876 200-209
GATCT 3195250 1.2017812 5.636945 130-139
AGCTT 3190060 1.1998291 6.4041967 150-159
GCATA 2688005 1.1954023 8.268927 210-219
TTCCA 2833395 1.1931802 7.481367 230-239
GTCCA 3329905 1.1874955 15.278599 70-79
CAATA 1908320 1.1849431 6.6553264 100-109
GTGGT 4913115 1.1820527 9.879198 50-59
TTGGC 4375410 1.1786289 7.6485195 60-69
ACTGT 3095105 1.1641152 5.7330184 230-239
ATACC 2331215 1.160766 7.158758 70-79
AGAGC 3067140 1.155098 7.426128 140-149
TGGTC 4264065 1.1486351 11.193573 50-59
TCTAC 2703085 1.138305 7.514239 180-189
TGCCC 3960145 1.1324717 6.199395 60-69
ATCCA 2261755 1.1261804 15.847529 240-247
GTCTA 2991240 1.12505 7.521287 180-189
TATCA 2139930 1.1237826 6.5773497 70-79
AGTTC 2982945 1.1219301 5.500696 160-169
AGGTT 3312065 1.1126051 23.602812 10-14
CTACC 2763340 1.1033489 8.978312 230-239
GCTTC 3656965 1.1029541 5.770018 150-159
TACTG 2927045 1.1009053 6.7516623 180-189
TATGT 2770775 1.0991127 7.774884 220-229
TCTGT 3451950 1.0980486 6.4247108 210-219
CCATA 2203780 1.0973133 9.922354 70-79
CGGCT 4287050 1.0949553 7.945928 130-139
ATATC 2080785 1.0927228 7.3702297 70-79
AAAAG 1655730 1.0857276 13.8681965 70-79
GAGCT 3408465 1.085627 6.658302 140-149
CTCGG 4240135 1.0829728 8.438006 130-139
AGGGG 4428240 1.0667824 5.0775814 60-69
CTGCG 4143585 1.058313 7.0037694 210-219
GGGGT 5139285 1.0470896 8.066918 10-14
TAGAT 2217310 1.039994 7.9954796 240-247
AACTT 1969680 1.0343761 6.070879 200-209
ATACG 2325635 1.03425 5.4613853 120-129
CTGCC 3606385 1.0313079 5.706829 200-209
GTCCG 4016980 1.0259768 5.03928 120-129
GAAGA 2181040 1.0243132 16.711412 35-39
ACCCA 2159410 1.0194763 13.523585 240-247
AGAGA 2165255 1.0168998 12.26167 35-39
GCGGT 4453835 1.0159998 5.935085 130-139
CATGG 3176255 1.0116659 5.2485504 190-199
TATAA 1543380 1.0107403 6.352629 240-247
TGCGT 3733900 1.0058216 8.296141 210-219
CTGAA 2241780 0.9969585 13.650318 35-39
AAAAT 1283435 0.9938116 5.2736936 8
ATGGG 3477380 0.98922616 28.643194 15-19
CTTCT 2770025 0.98655003 5.5049095 210-219
CAGCG 3250980 0.98178333 6.434033 160-169
ACTAA 1575350 0.97819024 13.012764 240-247
CGAAC 2317175 0.97706276 13.653579 70-79
AGAAA 1484785 0.9736322 5.036662 6
TGTAA 2061300 0.96682 7.017065 230-239
CGGAG 3573725 0.963927 6.6743736 210-219
CCAAT 1924025 0.95801675 11.139975 240-247
AGCTG 3000160 0.955578 5.1167645 90-99
TCCCC 2976225 0.9529286 5.0798826 80-89
CCCAA 2008900 0.9484191 10.34331 240-247
ACAAT 1501310 0.93221617 7.683865 100-109
TAGGC 2907295 0.9259998 5.7853727 160-169
GAGTT 2748635 0.9233349 5.2406473 160-169
TCGAA 2049765 0.9115661 17.181862 70-79
CCATT 2148170 0.90462285 5.3925643 50-59
CTATA 1717900 0.902154 5.029756 90-99
TGCAT 2395505 0.9009852 6.266371 210-219
CGTGG 3906890 0.89123183 6.783025 50-59
AAGAG 1869120 0.8778218 12.237545 35-39
CCAGC 2569940 0.86896586 5.885613 80-89
TTCTG 2718855 0.8648546 5.4223156 210-219
CATCA 1725650 0.85924125 5.4841075 230-239
TGAAG 2161325 0.85847116 9.030715 35-39
TGAAC 1922960 0.8551736 6.7526674 190-199
CGGTC 3330925 0.8507515 5.777168 130-139
CTTCC 2517050 0.8499764 5.305831 230-239
AGCGT 2657350 0.84638995 6.659853 160-169
GGTGG 4109985 0.83737767 19.726433 25-29
GTATG 2492045 0.83714 5.37952 220-229
ACGTC 2276640 0.81188494 10.366808 70-79
ATCAA 1305935 0.810901 12.360022 70-79
GAGGC 2990185 0.806531 12.774044 30-34
AACCG 1900885 0.80152947 5.965958 190-199
TAACC 1606855 0.8000904 6.192067 80-89
CTAAT 1484030 0.77933735 10.019193 240-247
AAAGG 1641215 0.7707875 9.015854 10-14
TGAGA 1924720 0.76449245 5.882872 240-247
GGGCC 3532735 0.7640999 14.976732 15-19
AAAGA 1155640 0.7577989 5.928713 8
AATCC 1520565 0.7571247 9.864623 240-247
AGCCA 1777930 0.7496841 6.021962 80-89
ATGAT 1588020 0.74483556 6.8591547 240-247
CGTCC 2581570 0.73824435 8.471345 70-79
GTCGA 2297145 0.7316615 12.245369 70-79
TCCAG 2022430 0.72122973 5.073581 240-247
GTAGC 2247235 0.7157647 5.673818 80-89
GGTCC 2793715 0.71354276 11.463337 50-59
GACCG 2348320 0.7091835 16.35576 45-49
TGGGC 3106775 0.7087112 16.243336 15-19
AAGGT 1781275 0.70751655 9.984017 10-14
CATAC 1381695 0.687978 8.484478 70-79
CAATC 1341770 0.66809845 9.62176 240-247
TAGGT 1981370 0.6655915 5.5834517 10-14
AAAGC 1260120 0.6626122 9.500446 70-79
TATGA 1409310 0.66101444 5.579658 240-247
AGGAT 1609970 0.6394748 5.5006666 10-14
GAGGT 2224005 0.63267285 6.9469233 10-14
GGAAG 1862295 0.6264052 5.5164547 30-34
AGACC 1476030 0.62238455 5.228087 240-247
ATAGG 1549320 0.61538476 5.293279 10-14
AATGG 1505585 0.59801346 6.808953 10-14
CCGTA 1661595 0.5925504 5.016117 80-89
GGGTC 2543545 0.5802283 5.8267155 70-79
CATCC 1442470 0.5759507 7.3466477 240-247
CGATG 1797755 0.5726012 12.580965 15-19
TAAAA 736115 0.5700013 6.0350027 8
AAATG 1008550 0.5593264 5.7834897 9
AAGAA 836310 0.5484016 5.5118475 9
GAAGG 1573095 0.5291293 5.52384 10-14
AAGGG 1572425 0.528904 6.468421 10-14
GAAAA 776895 0.5094408 5.9497705 8
TGATC 1330235 0.5003212 5.3738236 240-247
GGTCG 2188015 0.49912557 5.9210877 70-79
AAGCA 940265 0.49442202 6.2626557 70-79
GATCC 1356905 0.48389325 5.44442 240-247
ATAAG 846585 0.46950307 5.69034 9
TAAGG 1116645 0.4435277 5.8263645 10-14
CGGTT 1638335 0.44132748 8.218081 10-14
ACGAT 989570 0.44007885 5.8292494 10-14
TAAAG 751310 0.41666502 5.8204756 9
TGGGT 1626000 0.39120147 5.077538 10-14
AAGCT 874070 0.38871405 7.3999534 30-34
Produced by FastQC (version 0.10.1)