FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_17_SL248346.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_17_SL248346.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7015073
Filtered Sequences 0
Sequence length 251
%GC 54

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 32170 0.458583966268063 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25236 0.3597396634361467 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23333 0.33261236198112265 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23226 0.33108707493136563 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22086 0.31483635309283314 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21061 0.3002249584573104 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 20390 0.2906598406032268 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19671 0.280410481829626 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19207 0.27379615294096016 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19102 0.27229937592951636 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 18602 0.2651718663512126 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18446 0.2629480833627818 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18279 0.26056749516362837 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17874 0.25479421240520234 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17623 0.25121620259689387 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17448 0.24872157424448757 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17220 0.24547142987678106 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16897 0.2408670586891968 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16656 0.23743159907245442 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16655 0.23741734405329779 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16270 0.23192916167800393 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16184 0.23070323003053567 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15892 0.2265407644368063 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15684 0.22357572045223192 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15603 0.22242106390054672 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15283 0.21785945777043234 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14445 0.20591375171719523 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14083 0.20075343478250332 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13972 0.1991711276561199 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13913 0.19833008152588005 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 13855 0.1975032904147968 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 13763 0.19619182865238893 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13492 0.1923287184609483 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 13202 0.1881947629055321 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13146 0.1873964818327621 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13020 0.18560034941902956 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12794 0.18237871508963627 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 12755 0.18182276934252858 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12551 0.17891474543458064 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 12538 0.17872943018554477 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 12170 0.1734835831359132 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11437 0.1630346540941199 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 11378 0.16219360796388005 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11360 0.16193701761906112 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11333 0.16155213210183272 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11263 0.16055428076087022 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 11204 0.15971323463063036 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 10962 0.15626351999473134 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 10840 0.15452440765762523 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 10807 0.1540539920254572 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 10559 0.1505187472746185 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10409 0.14838049440112738 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 10101 0.14398994850089228 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9785 0.1394853624474043 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9727 0.1386585713363211 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9693 0.13817390068499644 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9559 0.13626372811801102 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 9525 0.1357790574666864 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 9519 0.13569352735174672 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 9295 0.13250040306066665 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 8847 0.12611415447850652 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 8620 0.1228782651299566 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 8313 0.1185019742488781 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7971 0.11362675769731832 No Hit
TTGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 7621 0.10863750099250571 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 7246 0.10329186880877789 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7231 0.10307804352142878 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7184 0.10240805762106824 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7162 0.10209444719962288 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 14054495 7.564186 65.71389 35-39
TATTA 6964885 7.1544347 123.97686 240-247
TGGAT 10622370 7.058526 77.70378 35-39
TCACC 10913195 6.4146266 74.23885 160-169
TCTCC 9688320 6.1328597 86.808075 20-24
CTGTG 10140730 5.8777523 86.772766 25-29
TCCCT 9145530 5.7892647 72.93246 15-19
AGTGG 10943635 5.636018 39.2334 100-109
CTGAG 10384785 5.5891333 49.990265 15-19
TGTAT 6350115 5.4444766 57.84874 240-247
TTACT 5845835 5.2379017 110.34138 240-247
ATTAC 5899060 4.907932 103.20898 240-247
TGGGT 8815030 4.889107 45.219215 100-109
CCTGA 8619425 4.8479867 51.779846 15-19
CTCTG 7749325 4.69399 73.115746 35-39
TTCAC 6407845 4.650258 73.84686 40-44
CTCCA 7785660 4.5763035 41.22036 80-89
GACAC 8707155 4.547413 45.85504 230-239
ACACG 8705665 4.5466337 48.384 230-239
TGAGA 7110925 4.387565 92.67924 15-19
TCTGG 7435705 4.3098702 133.93086 35-39
ATTCA 5139200 4.275739 110.15762 40-44
TACTG 6100275 4.236218 77.847 240-247
AAGGG 8833305 4.2241454 38.5912 90-99
CCCTG 8519360 4.1796465 84.93388 10-14
AGCCT 7373500 4.1472178 50.662334 30-34
CAGCC 9088850 4.1404395 55.385387 30-34
CCAGG 9399160 4.0972295 52.931812 80-89
TGGAG 7934855 4.086483 38.166687 90-99
GAAGG 8530755 4.079464 30.55978 150-159
ACCAT 5969605 4.022681 66.32211 160-169
GAGAC 7989130 3.992559 85.285515 8
TCAGT 5726180 3.976435 96.36256 7
TCCAG 7054765 3.9679456 40.5128 80-89
GGGTC 8738285 3.9254222 124.73314 8
CACCA 7170710 3.9136908 56.734425 160-169
ACTAC 5773345 3.890429 35.162514 130-139
GTATT 4517030 3.8728218 67.25742 240-247
TACTA 4597495 3.8250482 28.27247 130-139
CTGGG 8452495 3.7970393 132.29019 3
CACCT 6431520 3.7803593 43.99404 40-44
GACTC 6666960 3.7498252 52.053066 20-24
ACTGT 5332005 3.7027078 67.128944 240-247
GATTC 5323255 3.6966314 58.803265 40-44
AGACT 5705865 3.6792157 67.72918 15-19
TGAAG 5956855 3.675484 39.07198 150-159
GGACA 7170425 3.5834122 27.883387 220-229
GCTGG 7948725 3.5707352 33.632145 2
CAAGA 5905215 3.5356913 43.20524 180-189
CCTGT 5805385 3.5164895 62.475002 25-29
ACTGG 6459455 3.4765048 43.338165 60-69
ACTCT 4727540 3.4308388 68.50664 20-24
GAGTG 6609340 3.4038377 38.460403 90-99
TCCTG 5548980 3.3611774 64.17646 20-24
TATCT 3696610 3.3121839 48.558254 200-209
CTCCT 5211515 3.2989712 94.04771 20-24
GGTCC 6926120 3.2515197 142.74542 9
GGATT 4833555 3.2118802 56.01259 40-44
CTGCA 5708710 3.2108583 44.636757 25-29
AGCTG 5958920 3.2071147 36.513824 60-69
AGACA 5344200 3.199789 36.925003 170-179
CATCT 4343705 3.1522846 33.084156 170-179
GTCTC 5163225 3.1275146 32.797375 30-34
CCATC 5252100 3.087112 34.143993 45-49
GTCCC 6244000 3.063342 75.35833 10-14
CCTCA 5122050 3.0106702 82.00983 5
GGAGT 5726100 2.9489653 36.499268 90-99
GGCTG 6517590 2.927839 34.396645 90-99
AGGGA 6002305 2.8703423 39.478233 80-89
AAGAA 4157755 2.8539615 36.14017 180-189
ATCTC 3908875 2.8367224 47.951263 170-179
GTGTA 4267625 2.8358212 35.573177 230-239
TGTGC 4844495 2.807958 56.980156 25-29
GAGCT 5204195 2.8009188 29.457106 60-69
AGTAG 4530920 2.7956572 27.84144 50-59
GTGAA 4502735 2.7782664 38.771996 10-14
ATCTG 3978040 2.7624729 36.359367 200-209
GGAAG 5727730 2.7390387 29.577538 80-89
CAGGG 6502605 2.7123919 35.893356 80-89
GACAA 4524010 2.7087083 24.339777 180-189
ACCTT 3695090 2.6815763 71.28128 40-44
CTATG 3855830 2.6776066 27.927101 50-59
GAACA 4437130 2.6566894 34.21893 210-219
GGGAA 5462445 2.6121776 33.210403 80-89
ATGAA 3479125 2.5719018 27.157124 210-219
CACGG 5859600 2.5542846 43.803223 230-239
GAGTC 4726645 2.5438993 27.550966 150-159
CCCTC 4937195 2.531332 27.385332 20-24
CGCCA 5553855 2.5300672 39.7761 70-79
TGCAA 3914240 2.5239525 35.086975 200-209
AGGGC 6001990 2.5035734 32.564205 150-159
AGAAC 4135015 2.4758012 29.439835 190-199
CAGTG 4595270 2.473193 74.57671 8
AGGCT 4562240 2.455416 20.170847 25-29
TATAT 2386360 2.451305 24.089066 240-247
CCAAG 4660870 2.4341931 38.157513 180-189
ACGGC 5578450 2.4317272 48.29529 230-239
ATCAG 3771010 2.431596 25.857512 45-49
CTACA 3600400 2.4261672 19.474518 190-199
CTCTC 3761240 2.3809242 98.23153 20-24
GTGGA 4622600 2.3806581 25.975798 100-109
AAAAA 2891070 2.377577 19.107746 90-99
TCTGT 3177405 2.376274 24.327276 210-219
CACTG 4222340 2.374851 19.273432 60-69
GCAGC 5426680 2.3655684 34.269653 30-34
CCGCC 5943395 2.3616827 34.34936 70-79
CTGTC 3879325 2.3498194 47.774567 30-34
GTATC 3362205 2.3348181 22.889988 200-209
TGAAC 3613220 2.3298512 37.311737 210-219
ACAGC 4458190 2.328341 26.691404 210-219
GGGCT 5154035 2.3153014 42.568176 90-99
CTACT 3185640 2.3118615 22.623508 50-59
CTTCA 3171210 2.3013895 49.958866 45-49
GTGGT 4147245 2.3001993 31.184778 120-129
TATGC 3311430 2.2995584 21.146746 50-59
CAAAT 2938975 2.2704737 34.407997 200-209
GGCCG 6233470 2.2680068 25.139349 230-239
TCCAA 3358890 2.263423 42.483833 180-189
CAGGC 5191685 2.2631307 33.254353 70-79
TGGTG 4068865 2.2567272 33.400627 35-39
GCCTC 4594945 2.2543063 72.504555 4
TATCA 2643935 2.1997151 16.534502 170-179
GCCTG 4683030 2.198484 30.967764 210-219
GCTCC 4476575 2.1962335 34.155712 80-89
ACAAC 3481670 2.1785245 19.404842 180-189
AATGA 2943940 2.1762724 25.633953 210-219
GGGGG 6508885 2.1684458 94.28323 5
AAATG 2929215 2.1653872 32.539738 200-209
CCTTC 3396185 2.1498387 55.415165 45-49
CAGTA 3319995 2.1407757 20.882206 45-49
TACAT 2540385 2.113563 18.212706 190-199
GTGGG 4892070 2.1028898 29.957064 100-109
CCTCT 3317215 2.0998492 49.10553 35-39
GCTAT 3017675 2.095566 33.695957 50-59
TGCAG 3861235 2.0781322 43.283417 30-34
GCAGA 4153735 2.0758247 24.220583 140-149
GTAGT 3122040 2.074584 24.55687 120-129
GCTGT 3566615 2.0672753 19.195831 230-239
TCTAT 2280320 2.0431798 19.658543 110-119
CTCAC 3449855 2.0277772 54.972183 20-24
AACAG 3365785 2.0152318 32.814228 210-219
TGCAC 3574620 2.0105414 25.830074 25-29
AGAGT 3252060 2.0065782 29.776176 150-159
CAGAG 3981730 1.9898654 32.089886 170-179
AACTA 2575235 1.9894701 36.03057 130-139
GGCTC 4190465 1.9672457 19.66036 40-44
AGCAC 3754265 1.9607085 20.138712 130-139
TGAGC 3634425 1.9560621 17.690294 200-209
TCCGC 3978200 1.9517276 42.207794 70-79
TGTGA 2934815 1.950174 18.569616 210-219
ATGGA 3155580 1.9470483 11.88561 100-109
GGTCT 3352810 1.94335 36.1422 15-19
TGGTA 2922030 1.9416782 30.754782 120-129
CCGTG 4134675 1.9410545 20.018229 230-239
TCTCT 2473825 1.9334334 32.325756 35-39
TTCAG 2776275 1.9279305 46.8348 45-49
CTGTA 2772790 1.9255104 23.872704 190-199
ATATC 2313030 1.924407 13.314396 160-169
CAGAC 3657270 1.9100517 26.474918 140-149
GGTGG 4431235 1.9047972 17.859222 40-44
CATAT 2278775 1.8959074 14.142712 160-169
GCACA 3612575 1.8867092 18.267826 130-139
TGGGG 4383860 1.8844323 122.430664 4
GCCAG 4308880 1.8783032 35.4122 70-79
ACTAT 2256355 1.8772544 16.706572 130-139
ACCCT 3182285 1.8705035 43.379074 10-14
CCAGA 3574710 1.8669335 33.771973 170-179
GGATC 3449600 1.8565886 17.732292 70-79
CCTGC 3781965 1.8554536 49.819717 20-24
GTCAC 3293360 1.8523469 37.788563 160-169
AGGAC 3685940 1.8420445 20.484787 220-229
AGAGA 3209050 1.8385646 27.528809 170-179
TTCCA 2524555 1.8321033 23.12637 180-189
GATGG 3557140 1.831942 20.72084 100-109
CTATT 2041260 1.8289807 19.321388 110-119
TCCAT 2518170 1.8274693 26.114681 45-49
ACCAG 3495815 1.8257301 13.527357 190-199
CAGTT 2610020 1.812478 24.076979 190-199
CACAG 3469775 1.8121305 15.865707 140-149
GCTGA 3349800 1.8028759 26.790068 200-209
TGTCT 2409915 1.8022943 48.796673 30-34
TGGGA 3493835 1.7993394 16.560772 100-109
TCAGC 3195680 1.7974072 11.533099 45-49
GAGGA 3730040 1.78373 26.57249 220-229
GCCGA 4077315 1.7773607 19.257038 220-229
ATACT 2131840 1.7736597 26.234367 130-139
CAAGG 3530670 1.7644485 17.127344 80-89
TAGTG 2646510 1.7585965 25.712816 120-129
ACCTG 3101675 1.744534 30.704721 25-29
TTCTG 2326945 1.7402438 42.817955 30-34
AGTCA 2692360 1.7360686 28.614372 160-169
TAGTA 2164190 1.7229586 16.519203 120-129
GCAAA 2874490 1.7210737 31.476427 200-209
AGTTC 2470505 1.7155944 21.383837 190-199
CCTGG 3650780 1.7138863 33.114304 2
TGACC 3040065 1.7098815 14.276128 210-219
TACAA 2212735 1.7094247 17.28829 130-139
CTCAG 3030045 1.7042458 78.18779 6
CATGA 2635525 1.6994207 22.935402 60-69
CTGAA 2612950 1.684864 22.506817 200-209
GTGCA 3110540 1.674105 49.550804 25-29
ATGCA 2593935 1.6726029 16.210814 60-69
AGCTA 2582970 1.6655325 25.470434 50-59
GCCGT 3542775 1.6631826 29.237097 230-239
AACAC 2651845 1.6592927 22.386 190-199
GGATG 3199610 1.6478125 22.074522 100-109
GGAGC 3925195 1.6372925 13.070358 200-209
TCTCA 2226100 1.6155105 26.055428 100-109
TCTGC 2660415 1.6114904 33.306217 200-209
AGGGG 4029905 1.6085095 32.625793 90-99
ATTGG 2414725 1.6045762 20.077501 100-109
CAGCT 2832405 1.5930834 12.949657 50-59
GGGGC 4572205 1.5918566 28.668823 90-99
GGGCC 4363525 1.5876397 28.454805 2
TCTGA 2279380 1.5828713 21.310907 220-229
CGATT 2274830 1.5797117 34.836567 160-169
ACAAT 2013845 1.5557743 23.066366 180-189
CTCCC 3019560 1.548148 13.839009 190-199
GCACT 2716920 1.528129 24.77529 25-29
GTTCT 2043145 1.5279994 22.686098 190-199
CGTCC 3111730 1.5266327 18.915771 180-189
GGCTT 2628250 1.5233817 21.473274 25-29
CCATG 2700675 1.5189918 15.473869 160-169
CGGCC 3961325 1.5062296 31.504028 230-239
TTCTC 1926130 1.5053787 21.665367 190-199
AGTGA 2437625 1.5040575 85.210266 9
GTCCG 3199505 1.5020318 34.45338 70-79
GAGCA 2972375 1.4854412 7.718937 200-209
AGTTA 1859815 1.4806391 15.50955 50-59
TTAGT 1711875 1.4677314 25.101353 110-119
CTGGT 2528300 1.4654489 30.688911 35-39
TACGC 2598680 1.461625 24.567839 140-149
ACTCC 2478465 1.4568077 17.15115 140-149
CTTTA 1617690 1.4494596 41.39192 45-49
CATCA 2146540 1.4464685 30.201555 45-49
GTTAC 2054605 1.4267807 15.595304 50-59
CGTGT 2445530 1.4174739 23.737314 230-239
AAGAG 2463255 1.4112755 18.155834 150-159
GTGAC 2607750 1.4035015 14.898092 210-219
GGGGT 3262065 1.4022212 117.167366 7
GGCAG 3342175 1.394101 13.576764 150-159
CTACG 2464325 1.3860571 16.395452 130-139
CTCAA 2052885 1.3833579 18.081226 150-159
GTCCA 2443400 1.3742878 28.415583 180-189
TAGCA 2118175 1.3658267 11.533809 130-139
CAACT 2024760 1.3644058 17.30505 130-139
AACCC 2487915 1.357875 19.655682 140-149
GGCCT 2885700 1.3547138 69.26743 3
CAACC 2472325 1.3493662 14.860067 140-149
GAGAG 2816215 1.3467327 25.306505 220-229
CGACA 2573840 1.3442178 16.2344 70-79
AGCAG 2685445 1.3420483 12.281589 50-59
AAGCT 2056865 1.3262932 16.208572 200-209
CAATT 1589775 1.3226695 24.170378 180-189
TGGAA 2136875 1.3184893 19.049646 120-129
GTGCG 2926615 1.314697 17.097605 240-247
TTATT 1188025 1.3142623 20.584423 240-247
ATACA 1701180 1.3142284 21.48831 35-39
TCCGT 2162180 1.3096949 14.001234 150-159
CACGT 2323380 1.3067828 16.342876 180-189
ATCTA 1568490 1.3049606 19.92836 110-119
AGAGC 2610165 1.3044271 24.240185 220-229
ACAGT 2020420 1.302793 11.9971075 120-129
GAACT 2018845 1.3017774 14.034315 190-199
ACAGA 2173265 1.3012217 20.021149 140-149
TGTGT 1816700 1.3000827 14.867562 240-247
ATCAT 1558310 1.2964911 15.888728 110-119
CCATA 1918520 1.2928146 14.557915 160-169
CCTTT 1652670 1.2916545 48.217728 45-49
TGTCC 2131555 1.2911445 53.728607 15-19
TCACT 1778075 1.2903727 13.050011 190-199
ACATG 1998440 1.28862 14.351962 190-199
GTGGC 2861615 1.2854978 13.909063 40-44
TCAAG 1987595 1.2816269 22.475027 150-159
AATTC 1540420 1.2816068 24.133286 180-189
TTACA 1540215 1.2814362 9.236606 120-129
TGCGA 2362785 1.2716603 14.114326 240-247
CCTAC 2147135 1.2620564 17.208517 190-199
ATTAT 1227630 1.2610401 9.510633 50-59
ATATT 1225180 1.2585233 25.647638 240-247
CCAGT 2219225 1.2482008 18.471937 190-199
AGACC 2388970 1.2476671 97.91537 9
AACCA 1991705 1.2462348 16.802732 190-199
ACATA 1609690 1.2435488 20.446238 130-139
CTTCT 1587585 1.240787 23.679817 30-34
ACACA 1969015 1.2320374 7.744441 130-139
GATCT 1771480 1.23017 9.483702 220-229
ACGTC 2181785 1.2271428 18.13664 180-189
CGGCT 2597060 1.2192096 21.114601 230-239
TCGGA 2263165 1.2180445 92.010414 5
AGATC 1882690 1.2139829 12.433504 210-219
ATCCG 2146915 1.2075303 23.320175 70-79
GATTG 1809045 1.202104 23.066465 100-109
AGTCG 2226915 1.1985346 15.772663 150-159
TGAGT 1799570 1.1958077 20.790955 90-99
TGGAC 2219195 1.1943796 15.830525 80-89
CGCTG 2495075 1.1713319 12.404308 190-199
TACCA 1735155 1.1692524 10.641553 45-49
ACGCA 2225950 1.1625283 20.926453 140-149
GGGTG 2702525 1.1616992 14.563946 60-69
GTCGA 2158280 1.1615949 12.305562 150-159
ATTCC 1598290 1.1599005 22.61455 180-189
CTTCG 1914570 1.1597105 104.4604 3
AAGGC 2308650 1.1537453 28.521742 25-29
GAAGT 1867430 1.1522372 13.504997 140-149
ATTAG 1447055 1.1520319 24.70334 110-119
GATCC 2047015 1.1513416 23.159096 70-79
AGGCC 2621540 1.1427672 14.329087 2
GACCC 2453590 1.1177367 42.73873 10-14
GGACT 2069535 1.1138321 12.726703 90-99
TCGAG 2067245 1.1125996 11.970916 150-159
GCAAG 2222995 1.1109394 16.893732 25-29
AGCTC 1974940 1.1108031 12.034034 210-219
GCCCC 2789990 1.1086376 12.369108 80-89
GAACC 2119945 1.1071657 10.3309 180-189
ACAAG 1848215 1.1066011 17.209686 80-89
AGGTC 2054580 1.1057832 17.931002 10-14
GTATA 1385105 1.102712 10.08872 240-247
TTGGG 1987595 1.1023861 14.9610615 100-109
TTCGG 1899155 1.1007849 100.057594 4
CGCAG 2522465 1.099579 16.485786 140-149
GCTTC 1805925 1.093901 27.65067 2
CACAT 1617820 1.090185 13.820307 130-139
CCCCT 2124705 1.0893501 9.86694 70-79
CTTGA 1560975 1.0839889 22.08602 90-99
CCGAT 1923365 1.0817946 23.328222 160-169
TTGAG 1624510 1.079481 20.509726 90-99
GCACC 2360965 1.0755413 10.890443 130-139
CTCCG 2173160 1.0661647 10.040436 140-149
ATGGG 2064640 1.0632982 19.909103 100-109
ATCAA 1372625 1.0604067 12.358919 110-119
TAGAC 1635740 1.0547464 17.026203 170-179
GAAGC 2106145 1.0525436 12.657161 200-209
TACAG 1631580 1.052064 8.775179 120-129
GTAGA 1697935 1.0476556 16.501354 170-179
ATGAG 1693015 1.04462 19.862347 60-69
ACACC 1913760 1.044508 13.036397 40-44
CAGCA 1998305 1.043638 12.332571 45-49
GGAGA 2181690 1.0432987 81.49525 7
CTATA 1246925 1.0374233 13.032752 50-59
CGTGA 1926475 1.0368366 20.7695 150-159
ATGGT 1560100 1.0366809 9.542 35-39
GAGCC 2352490 1.0254843 17.89178 220-229
TGGCT 1766100 1.0236638 30.454557 40-44
GACAG 2037040 1.0180086 15.564347 70-79
GCTCT 1674555 1.0143265 16.940577 210-219
ACCGC 2225130 1.0136614 11.078102 220-229
GGGAG 2530095 1.0098704 14.005389 5
TGAGG 1954815 1.0067378 11.839434 220-229
AAACT 1298070 1.00281 16.854668 130-139
TTATA 975560 1.0021099 13.281837 110-119
GTAGC 1861330 1.0017753 11.028814 50-59
CGAGG 2399515 1.000895 14.510458 220-229
AGCCG 2294040 1.0000052 18.89535 220-229
TTTCA 1106230 0.9911884 7.1031446 100-109
ACTCA 1470390 0.9908377 11.974442 190-199
TAGTT 1155285 0.990521 14.386134 50-59
ATGAC 1527000 0.98462945 10.812826 160-169
AAGGT 1576615 0.9727992 20.706615 15-19
ATGAT 1221620 0.9725582 8.978655 120-129
CCGTC 1979035 0.97092587 10.261214 140-149
ACCAA 1538350 0.962565 16.560205 130-139
AACTC 1426440 0.9612215 15.808529 190-199
CGAGT 1775585 0.955627 14.7067585 160-169
TTTAG 1114065 0.95517975 29.26779 45-49
CCGAG 2190780 0.9549926 17.799782 220-229
CGTAT 1371170 0.9521825 7.4718447 240-247
GCCAA 1820920 0.9509966 14.423726 180-189
GGTAT 1429250 0.9497316 9.187343 110-119
GATAC 1468020 0.9465984 23.442965 35-39
GCCAT 1672030 0.94043165 7.784728 60-69
GGGAC 2253335 0.9399198 9.776819 90-99
TTTAT 849515 0.93978286 11.012975 240-247
GCAGT 1742055 0.937581 5.942075 50-59
CCAAC 1714715 0.9358715 13.834852 130-139
CAGAA 1557740 0.9326821 17.927446 140-149
ACAGG 1864250 0.9316569 15.109052 70-79
TATGG 1401315 0.93116885 12.354061 35-39
CATAC 1380680 0.9303857 14.06597 130-139
AATCA 1201705 0.9283643 13.82709 110-119
CGGAC 2124835 0.9262463 10.678891 220-229
AGCCC 2030685 0.92508173 11.365632 80-89
TACAC 1368425 0.92212754 21.62253 35-39
AACTG 1427125 0.92022866 11.342235 60-69
TAACA 1187335 0.9172629 8.451375 120-129
GAGAT 1483360 0.9152592 11.996141 210-219
TCATA 1086250 0.90374404 8.694404 110-119
AAGTT 1131835 0.9010784 17.45061 140-149
CCCAG 1977250 0.9007392 14.534343 80-89
TATTT 806390 0.8920755 10.837846 240-247
TGGTT 1246520 0.8920454 9.16101 50-59
CCCCA 1870105 0.8903068 16.69874 80-89
TCCTT 1138610 0.88988763 10.868506 45-49
ACGCT 1568290 0.8820832 15.126652 190-199
CATTA 1060165 0.8820417 11.975059 110-119
GTTAT 1024200 0.87813103 10.740313 110-119
GTCTG 1514895 0.87806076 15.769124 3
CGGAG 2090740 0.87209755 71.29942 6
AGTCT 1253380 0.87038547 15.0970335 8
GACTG 1616780 0.87015754 12.378886 90-99
AGAAG 1518580 0.8700418 15.63686 140-149
GATGA 1393050 0.85953635 6.5220475 60-69
GGTAG 1644790 0.8470737 11.847426 120-129
GATCA 1313610 0.8470327 9.312794 110-119
TCTTC 1081555 0.84529597 46.869717 2
CTATC 1163660 0.8444835 8.372867 110-119
ATAGT 1059840 0.8437616 15.192098 120-129
GGTTC 1454170 0.8428635 11.517262 160-169
GTTTC 1126500 0.8424714 5.04877 100-109
GCCTA 1497680 0.84236866 16.47117 190-199
CATGG 1563140 0.84128827 8.605743 200-209
ATCCA 1244675 0.8387373 9.821764 180-189
TTACC 1151895 0.8359455 9.810684 45-49
GTCTA 1190005 0.8263758 9.946246 30-34
ACAAA 1150810 0.8255228 12.559412 130-139
TCATC 1132350 0.8217614 10.590724 110-119
GCTTG 1415685 0.8205569 16.997492 90-99
TGGCA 1521540 0.8188989 5.8220177 100-109
ATATG 1026335 0.8170875 5.6899815 50-59
GGTGC 1814165 0.81496125 11.704235 60-69
ATATA 845590 0.8065403 10.303548 130-139
ACATC 1186885 0.7997949 5.3633842 110-119
TTATG 925375 0.7934002 11.197381 50-59
CCTCC 1540590 0.7898705 10.45747 140-149
TGATG 1182220 0.78558093 11.830411 120-129
GTTCA 1106595 0.7684534 13.838423 160-169
TATAC 922240 0.7672902 8.672729 130-139
CTGAC 1358875 0.7642979 9.517474 220-229
CGCCG 1985060 0.75478685 10.9075985 220-229
CCAGC 1649005 0.7512067 6.184398 80-89
TCAAC 1102050 0.7426279 7.5793896 110-119
ATCTT 825700 0.7398319 12.522053 3
GGGAT 1428550 0.7357092 13.0943985 100-109
ATAAA 830670 0.7356981 7.818078 130-139
CCCCC 1767210 0.7338568 13.38479 80-89
GGTTA 1096910 0.7288928 8.073462 50-59
CATCC 1237060 0.7271269 6.7012606 110-119
TTTAC 804755 0.7210651 13.345585 45-49
GACCG 1650110 0.7193068 9.221164 210-219
GCGGA 1723200 0.71878785 8.945539 220-229
GATTA 901460 0.7176719 5.5969405 160-169
ACGCC 1567655 0.7141477 12.720971 180-189
AAATA 804605 0.71261317 12.535525 130-139
TATTG 829920 0.7115588 6.507502 240-247
TTGGA 1070525 0.71136 6.239429 60-69
AATAC 920175 0.71087134 11.357643 130-139
AACGC 1359395 0.7099597 14.064742 180-189
GGGCA 1699590 0.7089396 13.5592 150-159
ATGCC 1256730 0.70684654 8.013183 50-59
GACCA 1351755 0.70596975 6.145941 170-179
TGCCA 1249300 0.7026675 7.4858913 60-69
TTTCT 724975 0.69956636 9.482292 4
CACAA 1116265 0.69846106 8.249116 130-139
ATTAA 731295 0.69752353 6.376029 110-119
TCCGG 1481325 0.69541925 11.407738 70-79
GGATA 1125575 0.69449955 15.22269 35-39
CAACG 1321505 0.6901712 14.323756 180-189
CAAAC 1092045 0.6833063 13.664143 130-139
GAAAT 923210 0.682472 7.204725 110-119
TATAG 850850 0.67738014 6.245888 120-129
CAGTC 1200525 0.67523414 5.338658 210-219
AGGCA 1344035 0.67168003 6.6009183 80-89
AATAA 758375 0.6716687 7.350599 130-139
GCCGC 1749320 0.6651506 11.894401 220-229
CACGC 1448310 0.65977985 12.305617 190-199
CCCGT 1338550 0.65670025 10.008143 140-149
GCGAC 1503495 0.6553952 7.2702475 70-79
CCGTA 1154285 0.6492264 6.3317084 230-239
AGATG 1051795 0.64897597 5.6071634 120-129
GACGA 1294310 0.6468301 9.658491 220-229
TCAAT 762615 0.6344845 8.378566 110-119
ACTTC 873340 0.6337944 29.32948 4
TCCAC 1076285 0.63262546 10.434148 180-189
TACTT 702900 0.6298024 10.926549 1
CACCG 1362795 0.6208234 8.964412 45-49
AAGCA 1031390 0.61753494 5.897222 120-129
CACGA 1181945 0.6172845 8.889876 180-189
CCCTA 1047255 0.615562 11.991698 110-119
AGTAA 831190 0.61444736 5.2198844 50-59
GGCCC 1601895 0.6090946 10.481705 70-79
CCGGC 1597580 0.6074539 9.280516 70-79
GAAGA 1054310 0.604047 6.201988 15-19
GGTGA 1169120 0.6021017 6.403205 120-129
TAGCT 862020 0.59861314 12.110956 50-59
AATGG 961860 0.59348446 6.5231614 120-129
CTTTG 791315 0.5917978 7.1886373 45-49
ACGAC 1133060 0.5917537 9.028279 220-229
TGATT 689975 0.5915724 5.9102163 50-59
GTTCC 962660 0.583111 6.89309 140-149
GGGGA 1454055 0.58037627 8.835435 4
TAATA 604710 0.57678425 8.075594 130-139
ACCCG 1265495 0.5764982 8.54623 140-149
GTCCT 943540 0.57152945 6.476531 45-49
ATTTC 634890 0.56886506 6.4652066 240-247
CGGTT 979130 0.56752163 11.422852 160-169
TTAGC 809490 0.5621347 9.528098 45-49
TTCAA 674210 0.5609328 5.4029117 200-209
ACCTA 832385 0.56091136 6.848154 130-139
TCAGG 1031060 0.5549207 5.3131404 150-159
AAGAT 750540 0.55482775 5.6514163 15-19
CCTAT 754745 0.5477285 5.276631 110-119
CCACG 1200170 0.5467392 7.8694477 180-189
CTGCG 1158885 0.54404736 11.906111 25-29
GGAGG 1355275 0.5409489 5.2988567 6
CGGCA 1238030 0.5396752 10.604167 70-79
AAATC 692900 0.53529245 7.7498565 110-119
CTGTT 713780 0.53381205 5.672698 240-247
GCTAC 944245 0.53108966 7.8316035 50-59
TATGT 603435 0.5173746 6.4597173 140-149
ACATT 617165 0.51347226 5.892542 110-119
ACCCC 1068100 0.5084938 8.8351555 140-149
GGGTT 900285 0.49932793 5.939396 100-109
TGACG 913060 0.49141258 8.596981 220-229
GTAAC 758195 0.48889396 6.685623 120-129
TATCC 666140 0.48342675 5.477025 110-119
AACAT 622475 0.48088637 5.5021386 110-119
CGCGG 1320450 0.48043698 6.430579 220-229
TGGCC 1015190 0.47658864 15.13308 2
GGGTA 921215 0.47442955 5.0252943 100-109
CAATC 700780 0.47222796 6.3029957 110-119
CCGGT 1000290 0.4695937 8.928649 160-169
TAAAT 492015 0.46929353 7.871222 130-139
ATGCT 671220 0.46611574 7.0831923 50-59
GGTTT 641875 0.45934418 5.414161 100-109
TGCTA 658255 0.45711234 6.3427877 50-59
CATAA 588945 0.45498314 5.7171793 110-119
CCGCG 1182310 0.4495542 6.6928887 220-229
ATTCT 497625 0.44587487 7.5142856 2
TTGGT 620260 0.44387588 5.348194 120-129
TGCGC 931900 0.4374875 8.826231 25-29
GCCTT 698320 0.42299265 8.14364 1
GGTCA 785425 0.4227189 5.375519 160-169
GCATG 781045 0.42036158 8.265875 60-69
GCGCT 886530 0.41618824 7.986885 25-29
ACGAG 827660 0.41362223 7.761517 180-189
CACTT 569875 0.4135659 6.9861145 3
ATGGC 764255 0.41132513 7.076819 1
CTAAC 593055 0.39963633 5.369792 110-119
CATGC 708455 0.39846984 7.0230517 60-69
CCGGG 1082275 0.39377856 5.275733 80-89
CCTAA 582870 0.392773 7.50416 110-119
AGCTT 560785 0.3894263 6.871022 1
TTTGA 448245 0.38431737 5.754782 45-49
GAGGC 919315 0.38346824 5.953009 35-39
TTCTT 389410 0.3757621 20.21892 1
GGTAA 603575 0.37241638 7.0305758 120-129
CTCGG 763445 0.35840502 5.1503034 6
TGGGC 776805 0.3489572 9.655302 1
CGAGC 797415 0.34760475 6.6388717 180-189
TGCTG 599370 0.34740576 14.2225485 1
GCTTT 417520 0.31224915 5.256162 45-49
CTCTT 398085 0.3111258 8.978297 1
CGCTT 504600 0.30565083 6.061497 1
GCGAG 724455 0.30218747 5.2878666 240-247
AACTT 355710 0.29594553 10.215414 3
ATTGT 344310 0.29520532 5.348432 240-247
GACTT 422685 0.29352543 6.3191085 1
TCGGT 490945 0.28456068 6.0574446 7
TTCCT 364015 0.28449818 6.1838627 4
GAGGT 546630 0.28151673 6.666497 7
CCCTT 438640 0.27766603 5.7872705 3
TAACT 329895 0.27446783 7.3154626 2
GTCTT 357940 0.26769125 11.7 1
TGTAA 335630 0.2672023 7.781838 25-29
TTGGC 455685 0.26412335 7.6536117 1
AATCT 314505 0.26166356 7.3832836 2
CGGGG 735890 0.25620708 8.813255 3
CCTCG 494380 0.24254565 5.7095437 5
TGCTT 294495 0.22024287 11.280327 1
TCGCA 337085 0.18959312 7.063898 7
TAGGC 293980 0.15822122 6.9278054 1
TTGCT 172890 0.12929861 5.740871 4
Produced by FastQC (version 0.10.1)