FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_15_SL248344.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_15_SL248344.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10260331
Filtered Sequences 0
Sequence length 251
%GC 54

[WARN] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 11590040 8.162342 57.22224 110-119
CACAG 15441420 7.1746264 40.4937 110-119
ATACA 10633790 7.096718 34.72276 110-119
TCCCT 17963290 6.9733114 104.79957 30-34
CAGTA 12981650 6.365057 37.57298 110-119
GGAGA 16100160 6.0061917 90.64333 35-39
AGTAA 10039230 6.003415 44.779316 110-119
CCCTG 17837250 5.879619 68.694115 30-34
GGTGA 17862280 5.765549 100.2056 45-49
GTAAT 10941230 5.6610746 37.844387 110-119
GTAGT 13614630 5.1753716 28.183159 80-89
AATAC 7676515 5.1231093 36.66203 110-119
AGACG 12213190 5.0847507 98.119194 40-44
ACAGT 10151570 4.9774356 34.097984 110-119
GTGAC 13606325 4.9013543 82.66929 45-49
GAGAC 11319315 4.7126007 137.23846 40-44
CCCCA 10950835 4.655917 99.47727 60-69
ACTCC 10357835 4.647157 118.04998 30-34
CTCAG 11288455 4.538176 33.668144 140-149
GGCCC 15900980 4.450546 59.141747 60-69
ACCAG 9531880 4.4288464 89.027534 45-49
TAGTA 8518925 4.407756 35.24986 80-89
GCACA 9266735 4.3056507 32.907703 110-119
GTGTC 13772245 4.2925444 23.436558 120-129
AAAAA 4558355 4.288135 27.319422 130-139
CGTGT 13706590 4.272081 22.920052 120-129
CACTC 9388870 4.2124205 95.337204 25-29
TGACC 10430360 4.1932054 140.55411 45-49
CTCCC 11224245 4.1290483 68.645035 30-34
AGTAG 9259340 4.068 28.664589 80-89
CCCAG 10264085 3.9102695 85.092606 60-69
GACCA 8312640 3.8623445 101.06676 45-49
ATGCA 7868190 3.8578668 91.785416 25-29
TGAGG 11578890 3.7374096 67.97227 35-39
CCCTT 9359075 3.6331732 36.313564 20-24
AGGAG 9686545 3.613582 90.8944 35-39
GACGG 11791660 3.6067696 106.22049 40-44
CAGGG 11773385 3.6011796 35.112957 50-59
ATGGT 9473320 3.6011226 25.037384 190-199
CCACT 8024385 3.600229 26.411646 240-247
ACGGT 9969160 3.5911527 94.16984 40-44
CTGAG 9926810 3.5758975 103.686905 35-39
TGTCC 10250195 3.5654452 20.246193 120-129
TACAC 6477845 3.5446596 25.67833 110-119
TGCAC 8633800 3.4709537 112.24682 25-29
AGATA 5767870 3.4491608 18.35008 150-159
GCCCC 10993120 3.4338543 73.081245 60-69
GGGTT 12288560 3.4319391 42.112526 50-59
TGGTG 12264975 3.425352 17.772451 190-199
GAGGA 9104510 3.3964527 119.60394 35-39
CTTGG 10654775 3.3208895 20.799957 20-24
CCGTG 11197630 3.3073175 23.243954 120-129
CTTCA 6974885 3.3022945 16.558245 200-209
TCTTG 8955600 3.28728 17.949749 170-179
CCTGA 8175045 3.2865255 81.58705 30-34
GGATG 10133220 3.2707794 68.048805 20-24
CTCTC 8382640 3.2541232 13.566793 130-139
CCAGG 9467035 3.2316842 39.751263 50-59
AGGCT 8963295 3.228814 24.755411 30-34
GCACT 7963070 3.2013073 86.71706 25-29
TCAGG 8750165 3.1520388 20.337448 140-149
CAGAT 6423300 3.1494203 17.24299 150-159
CCTGG 10640320 3.1427112 29.167938 50-59
TGTCT 8465705 3.107457 19.604805 180-189
TCGCA 7614975 3.0613666 23.234264 100-109
TCTCA 6448350 3.0530038 20.964943 130-139
GGTTG 10796965 3.0153677 73.76428 15-19
CGCAC 7848720 2.9900975 23.782639 100-109
TTCCC 7672720 2.9785337 49.17439 50-59
CGGAT 8217500 2.9601588 77.19786 20-24
TGGCC 9891685 2.9215946 43.620544 60-69
ACAGA 5149345 2.9180293 13.423249 140-149
GGTTC 9339040 2.9107997 47.486423 50-59
GCCCT 8721270 2.8747559 19.656683 20-24
GATGC 7953420 2.8650303 94.42778 25-29
AGGGT 8846765 2.85554 48.83293 50-59
TCTCT 6942430 2.8439703 15.150063 180-189
GTTGG 10063805 2.810611 52.73929 15-19
ACCAC 5419890 2.8104384 18.618547 230-239
GCCGT 9422590 2.7830436 21.987244 120-129
CGGTG 10470910 2.7711668 67.88482 40-44
TCACA 5058205 2.767836 11.144022 140-149
GACTC 6868855 2.761412 18.388975 190-199
GATGG 8532730 2.7541766 19.885416 15-19
AGTCA 5608035 2.749686 30.838442 70-79
CCAGT 6781180 2.7261648 36.203876 60-69
TTCTT 6280460 2.714972 20.188349 170-179
CGGCC 9677065 2.7085264 12.500783 120-129
TCAAA 3999550 2.669197 54.634106 70-79
GTTCC 7592540 2.641002 53.709316 50-59
AGATG 5876825 2.5819252 16.53307 180-189
GGCTG 9537005 2.5240052 13.205442 30-34
TCCAT 5298465 2.5085847 17.609896 70-79
TGGAG 7732790 2.4959733 16.979254 25-29
TGTTC 6783310 2.48991 12.894244 140-149
TCCTC 6403650 2.4858835 16.955172 130-139
GCGGA 8080030 2.4714758 85.280396 20-24
AGGGA 6558100 2.4465103 8.571655 150-159
GGCGG 10821480 2.4318285 42.323177 20-24
ACAGC 5138275 2.3874233 10.849608 120-129
GAGAT 5398305 2.371692 16.078901 180-189
TTGGG 8450770 2.360124 79.04124 15-19
GTTCT 6394510 2.3471954 17.268156 160-169
ACTCT 4944230 2.3408704 12.999252 200-209
CTGGC 7891140 2.3307166 34.888004 60-69
CTGGA 6379250 2.2979732 14.416716 180-189
AATAA 2822220 2.297132 9.401994 110-119
TACCA 4156670 2.2745187 15.461985 230-239
CACGG 6646620 2.2689023 9.270283 120-129
CAGCT 5614925 2.257308 11.797578 150-159
TGTAG 5888315 2.2383435 7.6365647 210-219
CATTT 4479815 2.2382016 17.504717 150-159
TTCAC 4709630 2.229798 18.231476 200-209
TCATT 4459610 2.2281067 16.18796 150-159
TCAGC 5491335 2.2076223 13.811553 140-149
TTCAT 4394980 2.1958163 15.60306 140-149
TTGTC 5919070 2.1726785 13.267281 170-179
TCTGC 6245610 2.172484 11.945042 210-219
GTGAA 4928700 2.1653755 17.385185 190-199
CAGAG 5181960 2.1574192 8.906192 140-149
ACTAC 3891905 2.12964 11.881656 230-239
CTACT 4487330 2.124549 7.312 230-239
GTCAA 4329555 2.122832 40.935608 70-79
TGGGG 8881040 2.1060586 52.270313 15-19
ATTTG 4695900 2.1022599 15.351862 150-159
GATAC 4284125 2.1005576 13.924832 150-159
CACTA 3814405 2.087232 14.481935 240-247
GTCTC 5987180 2.0825908 12.015091 180-189
TCTCG 5969955 2.0765994 16.688404 100-109
CTCTG 5950575 2.069858 14.571933 180-189
CTCGC 6242925 2.0578296 16.011484 100-109
ATCTC 4335595 2.0527093 9.174049 100-109
ACACA 3245425 2.0524886 13.211446 110-119
GTCCT 5890260 2.048878 14.341976 130-139
GCTGT 6573115 2.0487137 10.130721 140-149
GAACT 4176325 2.0477018 10.317855 160-169
TTGGA 5374555 2.043046 15.325778 170-179
TAGTC 4780990 2.0282667 25.69384 70-79
GAATC 4113055 2.0166798 14.03581 190-199
GTTCA 4735675 2.0090423 14.742931 140-149
GATAT 3865210 1.9998889 7.7106524 180-189
TACAG 4075730 1.9983789 13.246394 160-169
ATATA 2829635 1.9927843 10.04849 110-119
CAGGC 5808835 1.9829144 14.99541 140-149
ATGTA 3802680 1.9675353 7.726934 70-79
TGAAT 3802515 1.9674499 13.443963 190-199
CCTTC 5066870 1.9669483 13.908028 200-209
TTTGC 5352050 1.9645457 12.248854 150-159
TTGCA 4625690 1.9623828 14.1529875 150-159
AGCTC 4877600 1.9608891 11.630834 140-149
ACACG 4196540 1.9498599 12.66326 120-129
AATCG 3956685 1.9400097 12.923031 190-199
AATAT 2734390 1.9257077 12.514735 110-119
GAGTC 5327480 1.9190979 12.086605 210-219
TCGGC 6458715 1.9076375 7.880618 130-139
TGGAA 4332115 1.9032717 8.968284 170-179
ACGGC 5540715 1.8913885 13.983582 120-129
AACCA 2967645 1.8768134 14.001018 70-79
GGGGC 8339945 1.8741722 47.68595 15-19
CAGCC 4887480 1.861965 10.030032 120-129
ATATG 3561270 1.8426281 7.8756943 180-189
TACTA 3181705 1.837233 9.593539 230-239
GGGCG 8119240 1.824575 59.48341 20-24
CATAG 3709240 1.8186845 7.970822 210-219
CCAGA 3901800 1.8129133 26.41848 60-69
CTGTT 4892675 1.7959259 12.677519 140-149
GTTGT 5439320 1.7890193 6.7858224 210-219
CTCCA 3951585 1.7729224 7.6454363 150-159
AGTAT 3425105 1.7721753 11.357761 220-229
CGTAG 4891380 1.7620033 9.391806 210-219
GACGT 4890820 1.7618016 9.289993 170-179
GTCAC 4345285 1.7468882 8.252864 140-149
CCTTG 4970580 1.728975 23.638487 20-24
TGCAG 4774960 1.7200657 13.397216 150-159
GCAGA 4124390 1.7171185 14.141527 150-159
ATTGT 3833090 1.7159972 7.4283495 50-59
CACCA 3302395 1.712429 12.8398485 230-239
AGTCT 3994595 1.6946497 12.678339 210-219
GTCTG 5424605 1.6907454 8.948392 210-219
AGAAC 2955690 1.6749294 10.732126 160-169
TTCAG 3928740 1.6667117 8.999675 150-159
CAAAA 2135180 1.6469076 19.735214 70-79
ATAGT 3144950 1.6272209 7.005345 220-229
TGGTT 4945500 1.6265996 17.708067 10-14
GGTCA 4510890 1.6249408 7.2290554 130-139
TCTGG 5192680 1.618459 11.980234 180-189
TGACT 3814925 1.6184273 11.109387 190-199
TCCCA 3586895 1.6093001 10.236275 240-247
TTGGT 4879180 1.6047865 25.22981 25-29
GTCCC 4841335 1.5958291 20.600243 50-59
TCAGA 3247855 1.5924618 9.5099325 130-139
AACTG 3224710 1.5811137 10.1069565 160-169
GCTCC 4774370 1.5737557 6.2191124 150-159
CTGTG 5040890 1.571149 5.1831217 210-219
TGCTC 4510025 1.5687748 5.272281 150-159
TGTGC 5025430 1.5663301 7.9115033 220-229
CATGT 3678870 1.5607079 8.795689 70-79
GAGAA 3060725 1.5541408 9.884069 160-169
GTGCT 4975610 1.5508021 7.7286 220-229
AATAG 2591880 1.549933 8.923319 240-247
TTGTA 3458120 1.5481308 8.576403 210-219
ACTGA 3156500 1.5476694 8.227149 180-189
CTGGT 4949780 1.5427516 6.781741 160-169
GGCCG 6136520 1.5390049 12.931577 120-129
TAGTT 3431000 1.5359895 9.365412 220-229
ATAGA 2557375 1.5292988 9.433261 240-247
CAAAG 2682625 1.5201889 27.6052 70-79
GAACC 3263315 1.5162508 11.807551 70-79
GGACG 4890935 1.4960129 6.936831 170-179
GCTCA 3714440 1.4932764 10.5295515 140-149
GCTCT 4267640 1.484463 9.937354 130-139
CCACC 3483810 1.4811958 9.415192 230-239
CAGAC 3182505 1.4787036 15.672778 60-69
CCTCG 4484580 1.4782336 9.611776 130-139
ACCAT 2696045 1.4752686 11.19484 45-49
CCCAC 3467840 1.4744059 10.11359 240-247
GCTGA 3992725 1.4382842 21.435858 30-34
CGACT 3509925 1.4110574 8.206802 190-199
CTGCT 4048735 1.4083189 5.6609488 90-99
CACTG 3490895 1.403407 7.00826 230-239
CTCTT 3373955 1.3821425 7.8869896 200-209
AATTG 2657885 1.3752099 8.951385 170-179
TCGAC 3419550 1.3747251 8.842772 190-199
TATAC 2379410 1.3739583 6.372047 110-119
GCGTA 3805485 1.3708354 9.868728 210-219
CTTGA 3228940 1.3698317 7.9850626 200-209
GAGGG 4993020 1.3684697 7.841955 200-209
AGATC 2786220 1.3661168 6.64646 130-139
TGGAC 3779890 1.3616153 9.947351 170-179
GGGAG 4935935 1.3528241 6.250782 150-159
TGATA 2614550 1.3527879 7.147157 180-189
CCTCA 2999965 1.3459675 7.2503824 130-139
GTGGA 4144000 1.3375914 21.842201 25-29
ATCGG 3700035 1.3328496 7.819889 190-199
CCATC 2946025 1.3217666 10.3467655 240-247
GGAAT 3005910 1.3206168 8.4069605 170-179
ACGGA 3146990 1.3101948 8.900881 200-209
ACTTC 2748165 1.3011326 6.567361 220-229
ACCGT 3228975 1.2981101 18.811674 45-49
CTGAT 3035490 1.2877632 7.0339785 180-189
CTCGA 3198065 1.2856836 7.9301744 190-199
GTGTT 3900060 1.2827492 7.465129 160-169
GAATT 2475165 1.2806691 9.989948 170-179
TCCAC 2852810 1.2799448 9.1581 240-247
CCATG 3166070 1.2728211 7.926386 70-79
TATGG 3338630 1.2691238 5.8546352 190-199
GGAGT 3930800 1.2687753 6.9432163 210-219
AGCCG 3697390 1.2621478 8.967307 120-129
GGAGG 4574735 1.2538276 16.59477 30-34
GTCCA 3109350 1.2500185 16.544147 70-79
GGCTC 4227675 1.2486805 8.31992 130-139
GTGGT 4401270 1.2291831 11.008202 50-59
ACGTG 3400975 1.2251203 8.875079 170-179
ATAAC 1828265 1.2201371 7.23335 80-89
ACTAT 2111780 1.219419 5.945778 230-239
TTGGC 3910145 1.2187171 8.292019 60-69
ATAAA 1495005 1.2168518 5.105659 7
TGGTC 3863000 1.2040229 12.434161 50-59
ACTCG 2982000 1.1988214 8.1089 190-199
AAGTA 2001745 1.1970347 14.713611 70-79
GATCT 2805585 1.1902293 5.273276 130-139
AAAGT 1989350 1.1896226 20.187073 70-79
AGAGC 2835925 1.1806884 6.612252 140-149
GAAGA 2321385 1.1787271 18.364384 35-39
TTCCA 2473985 1.1713207 7.4718523 230-239
TGCCC 3539320 1.1666512 7.027749 60-69
TTGAG 3068400 1.1664004 7.662316 200-209
ACTGG 3233590 1.1648239 6.8737435 160-169
GCATA 2352470 1.1534439 7.5975957 210-219
ATATC 1994385 1.1516309 7.051468 70-79
AAAAG 1665800 1.1512934 13.218155 70-79
TCACG 2849245 1.1454513 9.24568 200-209
ACTGT 2684530 1.1388735 5.377273 230-239
ATACC 2072380 1.1340007 7.323276 70-79
CTGAA 2288400 1.1220298 14.237398 35-39
AGCTT 2639150 1.1196216 5.883125 150-159
TATCA 1933450 1.1164448 6.6087813 70-79
TCTGT 3033570 1.1135149 6.353026 210-219
AGTTC 2609330 1.1069709 5.4197183 160-169
CAATA 1646665 1.098942 6.1640253 100-109
CCATA 2001705 1.0953276 10.151887 70-79
AGGTT 2878515 1.0942189 22.546816 10-14
CGGCT 3690605 1.0900522 7.8148913 130-139
CTCGG 3684405 1.0882211 8.322656 130-139
CTTCT 2628375 1.0767152 5.385879 210-219
TCTAC 2267345 1.073486 6.926087 180-189
ATGGG 3297460 1.0643471 31.2098 15-19
TATGT 2376905 1.0640925 7.234583 220-229
GTCTA 2504810 1.0626298 7.0819135 180-189
CTGCG 3584810 1.0588048 6.8085327 210-219
AGAGA 2075595 1.0539227 12.631651 35-39
GCTTC 3020110 1.0505202 5.314422 150-159
TACTG 2466720 1.0464706 6.090809 180-189
ATCCA 1906825 1.0434095 14.190537 240-247
CTGCC 3153665 1.0395296 5.7035284 200-209
GTCCG 3489745 1.0307264 5.060393 130-139
AAGAG 2024095 1.0277724 13.578117 35-39
GAGCT 2846695 1.0254542 5.9157 140-149
GGGGT 4317125 1.0237674 7.8443227 10-14
CTACC 2273145 1.0198716 8.184428 230-239
GCGGT 3846540 1.0180016 5.644302 130-139
TAGAT 1961285 1.0147836 7.378539 240-247
TGCGT 3220145 1.0036573 8.072746 210-219
CATGG 2759005 0.9938659 5.1091943 190-199
AACTT 1713920 0.9896801 5.4689956 210-219
CAGCG 2876665 0.98198354 6.125561 160-169
TCCCC 2666045 0.9807545 5.0735044 80-89
ACGTC 2422625 0.97394186 11.389169 70-79
ATACG 1977990 0.96983194 5.02272 120-129
CGGAG 3144540 0.96183497 6.4833302 210-219
TGAAC 1950555 0.95638025 6.3101716 190-199
TATAA 1357905 0.9563112 6.168454 240-247
TTCTG 2596095 0.95293355 5.3968515 210-219
CGTGG 3572115 0.94537395 7.653852 50-59
ACAAT 1407095 0.93905914 7.090883 100-109
CGAAC 2011420 0.9345764 12.3783 70-79
ACCCA 1788135 0.9272223 12.105385 240-247
GAGTT 2426705 0.9224709 5.170307 160-169
CCATT 1944010 0.9204013 5.6842628 50-59
TAGGC 2550710 0.9188326 5.589257 160-169
TGTAA 1772655 0.9171851 6.2827888 230-239
GGTGG 3819060 0.9056558 22.181086 25-29
GTATG 2382085 0.90550923 5.1088123 220-229
ACTAA 1355430 0.9045792 11.601921 240-247
CCCAA 1723400 0.8936545 9.096613 240-247
TGCAT 2088150 0.8858677 5.85923 210-219
CATCA 1609125 0.88050884 5.5708203 230-239
TGAAG 1985465 0.8722944 9.380721 35-39
GAGGC 2850555 0.8719124 13.810545 30-34
TCGAA 1743370 0.85479504 15.720852 70-79
AGCGT 2372675 0.85469973 6.273584 160-169
CCAGC 2236440 0.85200816 5.481424 80-89
GACCG 2494250 0.8514417 17.507778 45-49
AAAGA 1230430 0.8503939 5.378126 8
GGGCC 3345470 0.8390252 16.92339 15-19
CTTCC 2155275 0.8366732 5.37951 230-239
CGGTC 2823790 0.83403087 5.353978 130-139
CCAAT 1523710 0.83377004 9.898229 240-247
ATCAA 1243290 0.82973987 12.134557 70-79
CAATC 1507350 0.82481796 8.516942 240-247
CGTCC 2439925 0.8042623 9.513877 70-79
TGGGC 2979435 0.7885189 18.291328 15-19
AACCG 1686275 0.7835026 5.5451903 190-199
TAACC 1431375 0.7832445 5.9385123 80-89
TGAGA 1759105 0.77284545 5.2768364 240-247
GGTCC 2611685 0.7713838 12.682023 50-59
GAAAA 1082765 0.74833727 5.3900766 8
GGAAG 2003230 0.7473083 6.5476594 30-34
AAAGG 1449820 0.7361735 8.415042 10-14
AGCCA 1566175 0.72769994 5.4780655 80-89
CTAAT 1257960 0.72639215 9.184196 240-247
CATAC 1322505 0.72367126 8.761943 70-79
ATGAT 1375460 0.7116734 6.4906635 240-247
GTCGA 1968545 0.7091215 11.467933 70-79
AATCC 1295725 0.7090173 8.804031 240-247
GTAGC 1936320 0.6975132 5.396454 80-89
AAGGT 1555280 0.6832969 9.478572 10-14
AAAGC 1179110 0.6681777 9.203787 70-79
CATTG 1552730 0.6587235 5.2028856 50-59
TAGGT 1707620 0.6491229 5.3951774 10-14
AGGAT 1448605 0.63643026 5.9562945 10-14
AGAAG 1243850 0.6315883 5.037066 9
GAGGT 1950300 0.62951374 6.654152 10-14
TATGA 1207190 0.62460923 5.4108973 240-247
CGATG 1717580 0.6187173 13.858001 15-19
TAAAA 753180 0.6130471 5.794904 8
ATAGG 1388885 0.6101929 5.1118593 10-14
CCGTA 1505760 0.6053445 5.035915 80-89
GGGTC 2273970 0.6018149 5.609243 70-79
TCAAT 1036495 0.59851015 5.697665 70-79
AATGG 1318780 0.579393 6.4019513 10-14
AAATG 954790 0.5709603 5.0569215 9
CATCC 1251685 0.5615823 6.8019686 240-247
GAAGG 1458620 0.54414064 5.41611 10-14
AAGCA 930010 0.5270178 6.166696 70-79
AAGGG 1372265 0.51192576 6.0983067 10-14
GGTCG 1858860 0.49195448 5.70886 70-79
TGATC 1151505 0.4885095 5.0853324 240-247
GATCC 1174015 0.4719766 5.1235485 240-247
ATAAG 783015 0.4682395 5.632259 9
ACGAT 945815 0.4637443 6.025969 10-14
CGGTT 1432695 0.44654357 7.923898 10-14
GAAGC 1032425 0.42983228 5.084702 30-34
TAAGG 974840 0.4282863 5.705308 10-14
AAGCT 850445 0.4169833 8.269717 30-34
TAAAG 667385 0.39909324 5.037718 9
Produced by FastQC (version 0.10.1)