FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_14_SL248343.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_14_SL248343.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11380873
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 13145790 8.6984 60.87987 110-119
ATACA 12011410 7.5187016 36.531662 110-119
CACAG 17588185 7.501322 41.83939 110-119
TCCCT 20208410 6.9018745 102.462494 30-34
CAGTA 14547795 6.5586996 39.092136 110-119
AGTAA 11366120 6.388106 47.476234 110-119
GGAGA 18082280 6.217159 95.204285 35-39
GGTGA 20224365 5.8962812 102.7288 45-49
GTAAT 12343205 5.8823705 39.398033 110-119
CCCTG 19911715 5.776314 67.35542 30-34
AATAC 8690640 5.440021 38.538246 110-119
AGACG 14051450 5.380826 102.4857 40-44
GTAGT 15432010 5.296862 30.389948 80-89
ACAGT 11693515 5.2718816 35.540604 110-119
GTGAC 15452230 5.017455 85.016365 45-49
GAGAC 12492955 4.78402 142.61028 40-44
CCCCA 12549375 4.7817698 101.866974 60-69
TAGTA 9898790 4.717441 39.17211 80-89
CTCAG 12792685 4.626398 34.14673 140-149
ACTCC 11240185 4.527347 116.28548 30-34
GGCCC 18276640 4.503453 59.03392 60-69
ACCAG 10390280 4.4314313 91.567375 45-49
GCACA 10176210 4.340131 34.190975 110-119
GTGTC 15704130 4.3238516 23.499422 120-129
TGACC 11888130 4.2992716 145.51003 45-49
CGTGT 15566100 4.285847 22.985935 120-129
AGTAG 10539270 4.266209 31.822369 80-89
CACTC 10374980 4.178858 94.8305 25-29
CTCCC 12574585 4.0627937 66.62116 30-34
CCCAG 11698860 4.002405 87.234116 60-69
GACCA 9198860 3.9232934 105.128624 45-49
ATGCA 8634315 3.8926778 92.99963 25-29
CCCTT 11054975 3.7756581 39.70095 20-24
TGAGG 12931435 3.7700753 69.42581 35-39
AGGAG 10849265 3.73026 95.52426 35-39
GACGG 13423575 3.702267 109.109085 40-44
ATGGT 10756225 3.6919515 25.639507 190-199
ACGGT 11325310 3.6774125 96.70627 40-44
AGATA 6539690 3.675505 19.72941 150-159
TACAC 7315130 3.6730907 26.429136 110-119
CTGAG 11175230 3.6286807 105.00262 35-39
CCACT 8986445 3.6195807 26.81064 240-247
TGTCC 11775535 3.6109922 20.366585 120-129
CAGGG 13021525 3.59138 34.820583 50-59
GAGGA 10249165 3.5239298 125.686295 35-39
TGGTG 14068145 3.4778006 18.152975 25-29
GCCCC 12632450 3.4667766 73.35865 60-69
TGCAC 9511895 3.4399202 111.711784 25-29
CTTGG 12378835 3.4082906 22.865059 20-24
AGGCT 10455030 3.3948264 28.691227 30-34
CCGTG 12906820 3.3618035 23.22755 120-129
GGGTT 13510680 3.339989 41.053436 50-59
GGATG 11276915 3.2877107 68.06214 20-24
TCTTG 10134690 3.2851877 17.998169 170-179
CCTGA 9065525 3.278493 81.70689 30-34
CAGAT 7247650 3.2675164 18.100578 150-159
CTCTC 9546750 3.2605467 13.656866 130-139
TCAGG 9966885 3.2363224 20.846844 140-149
CCAGG 10521775 3.232046 39.409904 50-59
CTTCA 7517560 3.2007449 16.914837 200-209
GCACT 8757855 3.167226 86.39373 25-29
TCGCA 8653005 3.1293077 23.503115 100-109
TCTCA 7309970 3.1123595 21.538689 130-139
CCTGG 11938605 3.1096153 28.72731 50-59
TGTCT 9576990 3.1044073 19.518587 180-189
ACAGA 5757840 3.0613687 14.079209 140-149
CGCAC 8920125 3.0517464 24.08887 100-109
GCCCT 10200690 2.9591815 21.326597 20-24
GGTTG 11946380 2.9532769 71.63594 15-19
CGGAT 9089950 2.9515746 77.051674 20-24
TGGCC 11139505 2.901476 43.1255 60-69
TTCCC 8490080 2.899657 47.96514 50-59
GATGG 9823630 2.8640149 22.170986 15-19
TCTCT 7908880 2.8553128 15.243104 180-189
ACCAC 6009050 2.854379 18.789991 230-239
GATGC 8754480 2.8426452 93.428665 25-29
GGTTC 10307455 2.8379736 46.210526 50-59
AGGGT 9714595 2.8322268 48.879692 50-59
GACTC 7745510 2.801118 18.469301 190-199
CGGTG 11896920 2.7822654 68.3412 40-44
TCAAA 4398535 2.7533214 56.627174 70-79
GTTGG 11082725 2.7397716 51.441635 15-19
CGGCC 11093585 2.733513 12.465033 120-129
GCCGT 10484105 2.7307653 21.997171 120-129
TCACA 5384450 2.703653 11.29878 140-149
TTCTT 7077870 2.7011273 20.230066 170-179
AGATG 6620890 2.6800814 17.248154 180-189
TCCAT 6291685 2.6788049 19.841562 70-79
GTTCC 8598200 2.6366558 52.17883 50-59
TGGAG 8959565 2.6121025 19.51443 25-29
AGTCA 5788520 2.609685 30.35377 70-79
GGCTG 11094775 2.5946724 14.978634 30-34
CCAGT 7158820 2.5889444 34.708187 60-69
TGTTC 7746185 2.510947 13.00024 140-149
AGGGA 7265495 2.4980664 8.772412 150-159
TCCTC 7309170 2.4963353 16.999998 130-139
ACAGC 5745980 2.4506478 11.459551 120-129
GCGGA 8871465 2.4467797 84.97745 20-24
GAGAT 6040825 2.4452758 16.790596 180-189
AATAA 3121100 2.4355428 10.678948 110-119
GGCGG 12004140 2.3845274 41.166264 20-24
ACTCT 5597070 2.3830593 13.069174 200-209
CTGGA 7285615 2.3656936 14.813701 180-189
GTTCT 7218555 2.3399143 17.094584 160-169
CACGG 7572155 2.325991 9.376172 120-129
TACCA 4605825 2.3126879 15.594755 230-239
TTGGG 9290305 2.2966657 77.3602 15-19
CATTT 5102080 2.2962818 17.9531 150-159
TGTAG 6685255 2.2946374 7.5941625 210-219
TCATT 5061135 2.277854 16.590944 150-159
CTGGC 8737470 2.2758245 33.850403 60-69
GTGAA 5605445 2.2690377 18.343077 190-199
CAGCT 6247020 2.2591975 11.746496 150-159
TTCAT 5005190 2.2526746 16.034603 140-149
CAGAG 5830485 2.2327108 9.111736 140-149
TTCAC 5215495 2.2205966 18.628784 200-209
TTGTC 6774415 2.195945 13.247334 170-179
GATAC 4869750 2.1954687 14.651163 150-159
ACTAC 4350015 2.18424 12.370565 230-239
ATTTG 5322145 2.150679 15.808559 150-159
CTACT 5033490 2.1431046 7.627802 230-239
GAATC 4735360 2.1348805 14.868776 190-199
CACTA 4203100 2.1104708 14.693609 240-247
TCAGC 5835525 2.110383 13.750688 140-149
CTCTG 6782975 2.0800133 14.669941 180-189
TGAAT 4362535 2.0790424 14.324617 190-199
TCTGC 6771950 2.0766325 11.955594 210-219
CTCGC 7154280 2.0754297 16.002884 100-109
TACAG 4601045 2.0743263 13.894418 160-169
ATGTA 4338175 2.0674329 8.719556 70-79
GCTGT 7503810 2.0660398 10.221355 140-149
TTGGA 5996670 2.0582883 15.459287 170-179
GTCTC 6708105 2.0570543 11.996071 180-189
GTCCT 6702120 2.055219 14.4322195 130-139
ATATA 3100940 2.0518517 10.8155365 110-119
ACACA 3445360 2.0402324 13.697599 110-119
TGGGG 9712800 2.0394802 51.19272 15-19
CAGGC 6607725 2.02974 15.389476 140-149
GTTCA 5304515 2.0278265 15.10711 140-149
AATCG 4493735 2.0259466 13.496074 190-199
TCTCG 6538040 2.0049036 15.576474 100-109
AATAT 3027095 2.0029895 13.448802 110-119
TAGTC 5229195 1.999033 24.77062 70-79
TTGCA 5221450 1.9960723 14.312132 150-159
GAACT 4409415 1.9879321 10.418543 160-169
ATCTC 4663315 1.9854953 8.00614 100-109
TTTGC 6109265 1.9803346 12.194415 150-159
AGCTC 5451470 1.9714918 11.762386 140-149
TGGAA 4864055 1.9689292 9.146546 170-179
GAGTC 6019320 1.9545182 12.398547 210-219
ACGGC 6355880 1.9523791 14.221803 120-129
CCTTC 5693330 1.9444699 13.917635 200-209
GATAT 4065505 1.9374874 7.7403326 180-189
AACCA 3271095 1.9370383 14.627158 70-79
GTCAA 4274795 1.9272403 38.742966 70-79
CCAGA 4506135 1.9218565 29.713486 60-69
TCGGC 7362095 1.9175844 7.870949 130-139
TACTA 3594675 1.9079764 10.127378 230-239
CAGCC 5552865 1.899742 10.430998 120-129
ATATG 3974465 1.8941007 7.973428 180-189
ACACG 4438340 1.8929421 13.09228 120-129
CATAG 4152640 1.8721683 8.101383 210-219
GACGT 5692940 1.8485402 9.286126 60-69
AAAAA 1996770 1.8376027 8.100155 8
CCTTG 5964515 1.8290308 25.926859 20-24
GGGGC 9168730 1.8212956 46.672108 15-19
AGTAT 3819680 1.8203351 11.8998165 220-229
CTGTT 5594000 1.8133103 12.762793 140-149
GTTGT 6175840 1.7974494 6.6729875 210-219
CGTAG 5481005 1.7797233 9.697189 210-219
GGGCG 8932925 1.7744548 57.634014 20-24
GCAGA 4615170 1.7673211 14.602253 150-159
ATTGT 4306385 1.7402104 8.1903 50-59
AGAAC 3268710 1.7379307 11.105133 160-169
TGCAG 5346875 1.7361703 13.555637 150-159
AGTCT 4517975 1.7271456 13.019547 210-219
CACCA 3588820 1.7047374 13.162903 230-239
GTCCC 5828165 1.6907288 22.281754 50-59
CAAAA 2282425 1.684926 22.180555 70-79
ATAGT 3520770 1.6778843 7.261187 220-229
GGTCA 5165240 1.6771921 7.381022 130-139
CTCCA 4153600 1.6729964 7.548101 150-159
GTCAC 4594565 1.661597 8.274608 140-149
TCCCA 4124465 1.6612613 10.585261 240-247
TTGGT 5673705 1.6513053 27.264402 25-29
TCAGA 3655130 1.6478717 10.010192 130-139
TGACT 4297695 1.6429363 11.23662 190-199
AACTG 3592210 1.619505 10.313321 160-169
AATAG 2880735 1.6190605 9.198994 240-247
TCTGG 5872160 1.6167941 12.005576 180-189
GAGAA 3373465 1.6104352 10.306816 160-169
TGGTT 5516360 1.605511 17.43599 10-14
CTGTG 5752255 1.5837804 5.2067122 210-219
ATAGA 2814440 1.581801 9.7772665 240-247
GTCTG 5744180 1.5815572 8.959102 210-219
TTCAG 4108245 1.5705127 9.095278 150-159
GCTCC 5392250 1.5642715 6.1040783 150-159
GAACC 3665560 1.5633533 12.097191 70-79
TGTGC 5675120 1.5625427 7.798612 220-229
CAGAC 3663555 1.5624982 17.832548 60-69
ACCAT 3106070 1.5596273 12.570502 45-49
TTGTA 3837280 1.5506451 8.460794 210-219
ACTGA 3439220 1.5505313 8.294835 180-189
GGCCG 7007555 1.5503393 12.899557 120-129
TGCTC 5043115 1.5464817 5.262244 150-159
CTGGT 5587340 1.5383741 6.6537714 160-169
GCTGA 4726885 1.5348549 24.462337 30-34
TAGTT 3784475 1.5293067 9.244172 220-229
GGACG 5544590 1.5292164 7.0160913 170-179
GTGCT 5536720 1.5244367 7.5275397 220-229
CCCAC 3982120 1.5173328 10.345412 240-247
CCACC 3976780 1.5152982 9.491415 230-239
CAAAG 2838160 1.5090127 27.734188 70-79
GCTCA 4161465 1.504969 10.532686 140-149
CCTCG 5160290 1.4969808 9.74934 130-139
GCTCT 4790660 1.4690659 9.833118 130-139
CATGT 3816705 1.4590619 8.713601 70-79
CGACT 3934570 1.4229138 8.158132 190-199
CCATC 3480445 1.4018615 10.754886 240-247
GTGGA 4804580 1.4007438 24.61929 25-29
TCGAC 3861945 1.3966494 8.846367 190-199
CACTG 3856095 1.3945338 6.9132037 230-239
ACCGT 3850625 1.3925556 20.88307 45-49
AATTG 2919525 1.3913505 9.083366 170-179
TATAC 2609885 1.385271 6.7782745 110-119
CTCTT 3813975 1.3769447 7.766812 200-209
TGGAC 4240045 1.3767744 10.032499 170-179
GCGTA 4237550 1.3759642 10.265704 210-219
CCTCA 3415470 1.3756907 7.3676925 130-139
TGATA 2872540 1.3689592 7.210294 180-189
ACGGA 3552635 1.3604368 9.454721 200-209
GGGAG 5437945 1.3466208 6.3044314 150-159
GTCCA 3710470 1.3418694 18.285059 70-79
GAGGG 5391790 1.3351912 7.829725 200-209
CCATG 3676340 1.3295264 8.851166 70-79
GTAGG 4546885 1.3256146 5.080562 160-169
ACTTC 3108805 1.3236331 6.6353583 220-229
GGAGG 5312155 1.3154708 18.658405 30-34
CTCGA 3609695 1.3054247 7.867779 190-199
GAATT 2733250 1.302578 10.117249 170-179
CTGCT 4241935 1.3007982 5.47182 90-99
TATGG 3776130 1.2961134 5.9231696 190-199
AGCCG 4213695 1.2943497 9.305582 120-129
TCCAC 3202370 1.2898579 9.153283 240-247
GTGTT 4422275 1.2870826 7.575709 160-169
AGATC 2842425 1.2814734 6.7001653 130-139
CTTGA 3350030 1.2806599 7.9631658 200-209
GGAGT 4384280 1.2782081 7.0382566 210-219
CTGAT 3336085 1.275329 6.849508 180-189
ATCGG 3920320 1.2729572 8.018461 190-199
TTGGC 4605810 1.2681273 8.861518 60-69
ATAAA 1617980 1.262587 5.651285 7
GTGGT 5101340 1.2611076 11.975333 50-59
TGGTC 4560125 1.255549 13.524185 50-59
ACGTG 3839400 1.2466819 8.885551 170-179
GGCTC 4779625 1.2449356 8.297185 130-139
ACTAT 2328095 1.2357029 6.2041235 230-239
AAGTA 2188550 1.2300316 15.470414 70-79
ATAAC 1961985 1.2281306 7.326896 80-89
TTCCA 2867045 1.2206992 7.422589 230-239
TGCCC 4189830 1.215454 7.663123 60-69
GGAAT 2993950 1.2119261 8.489369 170-179
ACTCG 3320400 1.2008028 8.10508 190-199
GATCT 3135315 1.198578 5.2930107 130-139
GCATA 2630555 1.1859543 7.70473 210-219
AAAGT 2110060 1.185918 20.268255 70-79
TTGAG 3443635 1.1819885 7.6569715 200-209
AGTTC 3078770 1.1769618 5.520769 160-169
ATACC 2340545 1.17524 8.13573 70-79
ACTGG 3618570 1.1749767 6.865198 160-169
CCATA 2324710 1.167289 11.255995 70-79
TCACG 3203235 1.1584308 9.487513 200-209
TATCA 2181085 1.1576731 7.276808 70-79
CAATA 1838700 1.1509587 6.6479225 100-109
ACTGT 3001995 1.147612 5.2674484 230-239
AAAAG 1722575 1.1417564 15.061952 70-79
ATGGG 3885105 1.1326768 34.932926 15-19
AGCTT 2935750 1.1222878 5.843178 150-159
TCTGT 3461035 1.1219039 6.339764 210-219
AGAGA 2299110 1.0975564 14.268148 35-39
CGGCT 4196305 1.0929997 7.852293 130-139
AGGTT 3183820 1.0928099 22.73821 10-14
GAAGA 2287160 1.0918516 19.411621 35-39
AGAGC 2849920 1.091341 6.6182313 140-149
CTCGG 4189115 1.0911272 8.338354 130-139
TCTAC 2517005 1.071663 6.9235287 180-189
GTCTA 2798245 1.0697218 7.133683 180-189
ATATC 2009475 1.0665864 7.741436 70-79
TATGT 2631370 1.0633367 7.422044 220-229
ATCCA 2106990 1.057967 14.5595875 240-247
CTGCC 3612840 1.0480713 5.7307105 200-209
CTGAA 2322365 1.0470105 15.80682 35-39
AACTT 1962160 1.0414724 5.702188 210-219
CTGCG 3988720 1.0389308 6.854232 210-219
TACTG 2705995 1.0344561 6.023041 180-189
GAGCT 3162290 1.0268192 5.893895 140-149
GCTTC 3340895 1.0244924 5.2040014 150-159
CATGG 3137775 1.018859 5.2367964 190-199
AAAAT 1303850 1.0174562 5.2055817 8
GCGGT 4348085 1.0168622 5.640262 130-139
CTACC 2505030 1.0089817 8.05022 230-239
TCCCC 3110070 1.00485 5.2886686 80-89
TAGAT 2104575 1.0029719 7.4675035 240-247
ATACG 2224110 1.0027134 5.17311 120-129
TGCGT 3622720 0.9974511 8.102134 210-219
TATAA 1505890 0.99642783 6.406137 240-247
GGGGT 4670940 0.9807975 7.3799877 10-14
ACAAT 1559000 0.9758766 7.656701 100-109
CGTGG 4160140 0.9729085 8.258398 50-59
CTTCT 2690760 0.97143483 5.187181 210-219
CGGAG 3507495 0.96737874 6.6005754 210-219
CCATT 2266185 0.9648715 6.1852164 50-59
CGAAC 2256210 0.9622686 12.670979 70-79
CAGCG 3087735 0.9484808 6.209449 160-169
GAGTT 2759380 0.94712573 5.2501755 160-169
TAGGC 2910295 0.94499457 5.69831 160-169
GAGGC 3404025 0.93884146 15.840018 30-34
TGAAG 2319300 0.9388334 10.516363 35-39
ACCCA 1971150 0.9363226 12.338255 240-247
AAGAG 1959270 0.9353223 14.375588 35-39
GGTGG 4435645 0.9313906 24.441511 25-29
ACGTC 2569310 0.9291756 12.495209 70-79
CCCAA 1952005 0.9272284 9.074584 240-247
ACTAA 1459750 0.9137499 12.084221 240-247
TGTAA 1911230 0.91082996 6.3358865 230-239
TGCAT 2363200 0.90341145 5.8425293 210-219
CATCA 1784480 0.8960274 5.6640496 230-239
ATCAA 1422665 0.89053607 13.870876 70-79
GGGCC 4015015 0.888275 18.538708 15-19
TCGAA 1960225 0.883744 16.343939 70-79
TTCTG 2668000 0.86483943 5.205678 210-219
CCAAT 1703775 0.8555036 10.021823 240-247
CGTCC 2922275 0.847741 10.212327 70-79
CGGTC 3219065 0.8384608 5.319453 130-139
TGGGC 3576445 0.8364031 20.099167 15-19
TGAAC 1848155 0.8332187 6.4078975 190-199
AGCGT 2555555 0.8298078 6.3447676 160-169
CCAGC 2414340 0.82599217 5.5247803 80-89
GACCG 2679965 0.82322323 19.315155 45-49
GGTCC 3136505 0.81695676 13.732649 50-59
CTTCC 2368670 0.80898315 5.1993995 230-239
GTATG 2348425 0.8060701 5.1913733 220-229
AACCG 1855475 0.79135597 5.7175183 190-199
TAACC 1557960 0.78228664 5.8464 80-89
TGAGA 1908180 0.77241546 5.6262 240-247
AAAGA 1155950 0.76618624 5.8135 8
AAAGG 1555475 0.7425575 8.806439 10-14
CATAC 1477105 0.74168754 9.659528 70-79
CTAAT 1386810 0.736089 9.415664 240-247
AGCCA 1718615 0.7329855 5.7083354 80-89
CCTTT 2013865 0.72705793 5.1593437 50-59
AATCC 1431445 0.7187605 8.761982 240-247
GTCGA 2201490 0.7148403 11.504892 70-79
GTAGC 2186060 0.7098301 5.467736 80-89
ATGAT 1486280 0.70831263 6.542223 240-247
AAAGC 1319725 0.70168066 10.246789 70-79
AAGGT 1687020 0.68289167 9.815265 10-14
CATTG 1786295 0.6828704 5.672656 50-59
AGGAT 1667595 0.6750286 6.5995426 10-14
CGATG 2042060 0.66307217 15.112696 15-19
TAGGT 1905510 0.65404457 5.356726 10-14
GGAAG 1895835 0.65183747 6.592828 30-34
AGAAG 1348110 0.64356494 5.525729 9
GAGGT 2178060 0.63499916 6.645628 10-14
CCGTA 1746275 0.6315299 5.355919 80-89
TATGA 1319765 0.628957 5.537045 240-247
ATAGG 1546975 0.62620264 5.244475 10-14
CAATC 1234560 0.6199002 8.517348 240-247
TAAAA 753120 0.5876954 5.683026 8
AATGG 1435865 0.58122617 6.616542 10-14
CATCC 1434255 0.57769245 7.115061 240-247
AAATG 1012705 0.56917113 5.417685 9
AAGAA 850245 0.56355894 5.5111904 8
GGGTC 2389670 0.55885863 5.525996 70-79
GAAAA 818140 0.5422792 6.033882 8
AAGCA 1009375 0.5366715 6.905053 70-79
GAAGG 1555695 0.53488845 5.515723 10-14
AAGGG 1492355 0.51311046 6.234884 10-14
ACGAT 1091015 0.49187106 6.680133 10-14
TGATC 1280105 0.48936257 5.0847907 240-247
TTGCC 1589805 0.487517 5.362853 60-69
ATAAG 862390 0.4846895 5.8147783 9
GGTCG 2068340 0.483711 5.592561 70-79
GATCC 1307000 0.47266877 5.119668 240-247
CGGTT 1618215 0.4455465 7.8149705 10-14
GAAGC 1155295 0.44240567 5.3765984 30-34
TAAGG 1085650 0.4394621 5.9415584 10-14
TACCG 1200565 0.43417716 5.127812 70-79
AAGCT 938520 0.4231205 8.977082 30-34
TAAAG 719165 0.4041927 5.626645 9
Produced by FastQC (version 0.10.1)