FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_12_SL248341.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_12_SL248341.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9945621
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 11352350 8.425232 59.846645 110-119
CACAG 15129480 7.3334403 41.584007 110-119
ATACA 10365515 7.2971106 36.81995 110-119
TCCCT 17770535 7.0733466 107.4668 30-34
CAGTA 12834930 6.5586333 38.71969 110-119
AGTAA 9838195 6.1876516 46.51252 110-119
GGAGA 15715160 6.0799975 92.13718 35-39
CCCTG 17813125 6.0086775 70.29233 30-34
GTAAT 11010580 5.9315224 39.127525 110-119
GGTGA 17454435 5.7841015 100.68502 45-49
AATAC 7549145 5.3144436 38.751354 110-119
GTAGT 13216160 5.1679835 26.599066 80-89
AGACG 11816725 5.1171875 100.09066 40-44
ACAGT 9924460 5.0713863 35.275265 110-119
GTGAC 13377925 4.962134 83.33205 45-49
ACTCC 10192140 4.736356 121.99773 30-34
GAGAC 10937110 4.7362733 139.71738 40-44
CCCCA 10683000 4.70908 100.799484 60-69
CTCAG 11047165 4.586487 34.514755 140-149
ACCAG 9441110 4.576219 92.97603 45-49
GGCCC 15314435 4.3777814 59.591263 60-69
TAGTA 8101485 4.3643603 33.477085 80-89
GCACA 8982630 4.3539886 34.03397 110-119
CACTC 9351985 4.34593 98.61576 25-29
GTGTC 13571820 4.311847 23.70973 120-129
CGTGT 13475580 4.2812705 23.126577 120-129
TGACC 10189530 4.2304196 142.10036 45-49
CTCCC 11135175 4.20422 70.21882 30-34
AGTAG 8964930 4.0927687 27.428728 70-79
ATGCA 7831145 4.0017056 95.373184 25-29
GACCA 8210015 3.9794931 105.01757 45-49
CCCAG 10038525 3.9533308 86.094536 60-69
TGAGG 11421255 3.784809 68.538895 35-39
CCACT 7988725 3.712414 27.48365 240-247
CAGGG 11733645 3.6883063 36.081585 50-59
AGGAG 9502390 3.6763551 92.59211 35-39
TACAC 6379890 3.649074 27.175621 110-119
GACGG 11590870 3.6434274 107.03901 40-44
ATGGT 9299145 3.6362927 25.344662 190-199
ACGGT 9765900 3.6223626 94.76289 40-44
CTGAG 9686215 3.5928063 104.602585 35-39
TGCAC 8565845 3.556309 116.03567 25-29
TGTCC 9980750 3.549262 20.556988 120-129
GGGTT 12344085 3.5037613 43.02899 50-59
AGATA 5568265 3.5021143 18.7534 150-159
CCCTT 8738545 3.4782722 33.726746 20-24
GAGGA 8933500 3.4562588 121.95139 35-39
GCCCC 10714350 3.428219 73.1955 60-69
TGGTG 11905025 3.3791385 17.840172 190-199
CCTGA 8130770 3.375678 84.2117 30-34
AAAAA 3284890 3.3229663 19.678146 130-139
CCAGG 9435080 3.3196266 40.923347 50-59
GGATG 9969385 3.303684 69.80382 20-24
GCACT 7949420 3.300386 89.47703 25-29
TCTTG 8796695 3.2978468 17.95687 170-179
CTTCA 6712945 3.288729 16.511284 200-209
CCGTG 10806300 3.2566133 23.47481 120-129
CTCTC 8127370 3.2350016 13.458059 100-109
CTTGG 10152430 3.2254863 18.913105 20-24
AGGCT 8622900 3.1984017 23.281464 30-34
CCTGG 10613115 3.1983945 29.821228 50-59
TCAGG 8582820 3.1835356 20.603481 140-149
CAGAT 6182745 3.1593752 17.359924 150-159
TCGCA 7522340 3.1230738 24.025349 100-109
TGTCT 8302770 3.1126766 19.721054 180-189
GGTTG 10801675 3.0659618 74.82915 15-19
TTCCC 7696955 3.06368 50.72932 50-59
CGCAC 7740380 3.0482843 24.688457 100-109
TCTCA 6210710 3.0426798 21.268501 130-139
CGGAT 8202665 3.0425284 79.38757 20-24
ACAGA 4982215 2.9723284 14.329909 140-149
GGTTC 9352235 2.97126 48.612476 50-59
GATGC 7912675 2.9349658 96.988106 25-29
TGGCC 9723175 2.9302 43.87427 60-69
AGGGT 8804505 2.9176624 50.39301 50-59
ACCAC 5314140 2.8831441 19.1279 230-239
GTTGG 10047725 2.85196 53.481213 15-19
GACTC 6810980 2.8277366 19.459978 190-199
TCTCT 6673825 2.8004985 14.917293 180-189
GCCCT 8283680 2.7942297 18.366625 20-24
AGTCA 5444855 2.7823143 31.948074 70-79
TCAAA 3942920 2.7757351 57.28584 70-79
CCAGT 6675820 2.7716215 37.188572 60-69
CGGTG 10238580 2.756635 67.44073 40-44
GCCGT 9123540 2.7494926 22.219824 120-129
TTCTT 6120760 2.7077088 20.355835 170-179
GTTCC 7578920 2.6951454 55.077335 50-59
CGGCC 9423815 2.6938899 12.777739 120-129
GATGG 8081170 2.6779618 18.64855 15-19
TCACA 4575485 2.6170175 11.75722 140-149
AGATG 5653540 2.5810163 16.651003 180-189
GCGGA 8045535 2.5290005 87.653404 20-24
AGGGA 6517425 2.5215096 9.03875 150-159
TCCAT 5058435 2.4781704 16.410673 70-79
GGCTG 9163205 2.467101 12.135205 30-34
GGCGG 10787870 2.4614415 42.85023 20-24
TCCTC 6155580 2.4501543 16.789192 130-139
TGTTC 6529100 2.4477346 12.802542 140-149
TGGAG 7328140 2.4284205 15.786045 25-29
ACTCT 4895965 2.398575 13.580384 200-209
ACAGC 4938010 2.3935127 11.061722 120-129
TTGGG 8415645 2.3887076 80.34757 15-19
CTGGC 7873175 2.3726792 35.550053 60-69
TACCA 4125325 2.3595417 15.9001045 230-239
GAGAT 5151895 2.3519998 16.229298 180-189
GTTCT 6234820 2.33741 17.422943 160-169
CAGCT 5596720 2.323608 12.298113 150-159
AATAA 2677035 2.3195548 9.5846195 110-119
CACGG 6541730 2.301634 9.657254 120-129
CTGGA 6155170 2.2830725 14.255343 180-189
TGTAG 5797035 2.2668445 8.035969 210-219
TCAGC 5359410 2.2250834 14.383485 140-149
CATTT 4302545 2.222166 17.379309 150-159
TCATT 4294185 2.2178483 16.221903 150-159
CTACT 4516155 2.212503 7.605535 230-239
ACTAC 3856705 2.2059004 12.334029 230-239
TTCAC 4477555 2.1935923 18.134605 200-209
CAGAG 5064105 2.1929913 9.546484 140-149
TTCAT 4240090 2.1899095 15.897918 140-149
CACTA 3776985 2.1603034 14.960935 240-247
GTGAA 4706465 2.1486473 17.372902 190-199
TCTGC 6012445 2.13809 11.965968 210-219
TTGTC 5693050 2.1343024 13.188376 170-179
TGGGG 8857405 2.1305733 52.959167 15-19
GATAC 4151650 2.121488 14.2504225 150-159
CTCGC 6195790 2.089948 16.50097 100-109
ATTTG 4519125 2.085239 15.333999 150-159
TAGTC 4736505 2.073115 26.468557 70-79
TCTCG 5821830 2.070305 16.816242 100-109
GAACT 4031275 2.0599768 10.941736 160-169
TTGGA 5257000 2.0556717 15.670439 170-179
ATCTC 4188935 2.052195 8.858693 100-109
GCTGT 6427205 2.0419607 10.0425625 140-149
CTCTG 5727480 2.0367532 14.303491 180-189
GTCTC 5708955 2.0301657 11.705315 180-189
ACACA 3030935 2.0239537 13.3390875 110-119
GTCCT 5666795 2.0151727 14.236891 130-139
AGCTC 4853310 2.0149643 12.35423 140-149
GATAT 3727035 2.0077953 8.120309 180-189
GAATC 3924770 2.0055528 14.053581 190-199
ATATA 2698240 2.0025191 10.120152 110-119
GTCAA 3914055 2.0000772 40.579563 70-79
GTTCA 4554745 1.9935608 14.799049 140-149
ATGTA 3697410 1.991836 7.149559 70-79
TACAG 3888225 1.9868784 13.415485 160-169
ACACG 4082840 1.9790015 13.37894 120-129
CAGGC 5615125 1.9756185 15.128563 140-149
TGAAT 3633640 1.9574823 13.434845 190-199
AACCA 2907440 1.9414881 14.957885 70-79
TTGCA 4421900 1.9354159 14.024064 150-159
AATAT 2604665 1.933072 12.757816 110-119
ACGGC 5493380 1.9327838 14.4090185 120-129
AATCG 3782010 1.9326026 12.9011755 190-199
CCTTC 4849825 1.9304143 13.718564 200-209
TACTA 3178670 1.9166862 9.923163 230-239
TTTGC 5076835 1.9032857 12.057532 150-159
GGGGC 8323245 1.8990943 48.377327 15-19
GAGTC 5106500 1.8941007 12.004218 210-219
ATATG 3508925 1.8902968 8.40005 180-189
TGGAA 4137140 1.8887327 8.969836 170-179
CAGCC 4761215 1.875042 10.146216 120-129
TCGGC 6200605 1.8686296 7.8040605 130-139
GGGCG 8087760 1.8453642 60.254578 20-24
CATAG 3608015 1.8436913 8.13893 210-219
GTTGT 5353640 1.7931249 7.050982 210-219
AGTAT 3304395 1.7801144 11.54498 220-229
CTGTT 4721235 1.7699727 12.589892 140-149
CTCCA 3781810 1.7574325 7.738681 150-159
GACGT 4735150 1.7563597 9.671221 170-179
GTCAC 4223310 1.7534051 8.615648 130-139
CGTAG 4709985 1.7470255 9.587855 210-219
CCAGA 3595515 1.7427894 25.395811 60-69
AGAAC 2914705 1.7388774 11.491785 160-169
CCTTG 4875665 1.7338387 21.630693 20-24
TTGTA 3732380 1.7222148 9.215562 210-219
CACCA 3174085 1.7220744 13.221045 230-239
GCAGA 3917455 1.6964389 14.136826 150-159
ATTGT 3671000 1.6938926 7.439601 50-59
TGCAG 4560795 1.691688 13.38809 150-159
AGTCT 3854725 1.6871698 12.816642 210-219
GGTCA 4514530 1.6745272 7.672472 130-139
TTCAG 3805085 1.6654431 9.517734 150-159
TGGTT 4952895 1.6589011 17.98422 10-14
TCCCA 3568300 1.6582129 10.6863365 240-247
TGACT 3786175 1.6571662 11.688982 190-199
GTCTG 5189630 1.6487758 8.937107 210-219
ATAGT 3055095 1.6458136 7.272683 220-229
AACTG 3204370 1.6374292 10.793879 160-169
CAAAA 1988880 1.6346434 20.379139 70-79
TCAGA 3157720 1.6135911 9.826447 130-139
GAGAA 3023850 1.6117029 10.444125 160-169
GCTCC 4735590 1.597397 6.26831 150-159
TGCTC 4481095 1.5935254 5.1880355 150-159
ACTGA 3114855 1.5916871 8.856059 180-189
CTGTG 5005320 1.5902194 5.2137012 210-219
TGTGC 5004220 1.5898702 8.004834 220-229
GTGCT 4996070 1.5872808 8.06286 220-229
TCTGG 4985430 1.5839002 11.7610235 180-189
CAAAG 2654205 1.583466 29.067568 70-79
CTGGT 4966735 1.5779607 7.0946126 160-169
TAGTT 3409625 1.5732875 9.77575 220-229
AATAG 2496735 1.5703009 9.3306465 240-247
ATAGA 2482255 1.5611937 10.051973 240-247
TTGGT 4656865 1.5597498 23.198095 25-29
GTCCC 4602605 1.5525388 19.455292 50-59
GAACC 3189765 1.5461175 12.455001 70-79
GGCCG 6043910 1.5435526 13.115087 120-129
GGACG 4910105 1.5434225 7.266689 170-179
GCTCA 3711080 1.5407408 11.102106 140-149
CCACC 3437975 1.5154638 9.676991 230-239
CCCAC 3437895 1.5154287 10.387821 240-247
ACCAT 2610050 1.4928573 11.180832 45-49
GCTCT 4112025 1.46228 9.828562 130-139
CCTCG 4315040 1.4555383 9.540451 130-139
AAATA 1679315 1.4550662 5.257428 9
CGACT 3467225 1.4394988 8.656386 190-199
CACTG 3435665 1.426396 7.19715 230-239
TCGAC 3395805 1.4098471 9.403191 190-199
TGATA 2614680 1.4085573 7.6276164 180-189
CAGAC 2901350 1.4063193 14.649889 60-69
CTCTT 3336920 1.4002523 8.25736 200-209
TATAC 2319015 1.3983282 6.5548644 110-119
TGGAC 3755260 1.3928993 10.351895 170-179
CATGT 3180765 1.3921851 7.196216 70-79
GAGGG 4947270 1.3893449 8.182857 200-209
CTGCT 3901635 1.3874631 5.826216 90-99
GCTGA 3738700 1.3867567 19.694542 30-34
GGGAG 4891060 1.3735594 6.574221 150-159
CTTGA 3120085 1.3656261 8.459763 200-209
AATTG 2532570 1.3643239 8.967894 170-179
GCGTA 3668175 1.3605976 10.037386 210-219
AGATC 2631410 1.3446474 6.8229055 130-139
CCTCA 2879680 1.3382065 7.2425013 130-139
CCATC 2852585 1.3256154 10.51026 240-247
CTCGA 3174665 1.318036 8.402035 190-199
CTGAT 3007785 1.3164736 7.42738 180-189
ACTTC 2686920 1.3163451 6.5148034 220-229
GTAGG 3972235 1.3163309 5.0628114 160-169
ACGGA 3033885 1.3138121 9.058285 200-209
TCCAC 2804870 1.3034419 9.542623 240-247
TATGG 3303155 1.2916498 6.072924 180-189
ATCGG 3457785 1.28256 7.6893554 190-199
GTGGA 3850295 1.2759221 20.035795 25-29
GAATT 2359530 1.2711052 9.918563 170-179
ACGTG 3417830 1.2677399 9.217193 170-179
GGAAT 2773475 1.2661773 8.353002 170-179
GTGTT 3775245 1.2644641 7.4647245 160-169
ACTAT 2096515 1.2641643 6.2357 230-239
CCATG 3042020 1.2629653 7.3379164 70-79
AGCCG 3563935 1.2539303 8.993986 120-129
GGAGT 3747870 1.2419801 6.8749065 210-219
ACTCG 2987570 1.240359 8.6192665 190-199
GGAGG 4403425 1.2366166 15.454848 30-34
ACCGT 2976070 1.2355845 17.228388 45-49
ATAAA 1424670 1.2344254 5.015484 7
GGCTC 4085175 1.2311183 8.225125 130-139
AAAGT 1955560 1.2299334 21.105753 70-79
ATAAC 1727855 1.2163745 7.4583006 80-89
AAGTA 1932230 1.2152601 15.546327 70-79
GATCT 2759335 1.2077299 5.433378 130-139
ACTGG 3249680 1.2053698 7.265684 160-169
TTGAG 3080970 1.2047675 8.119201 200-209
GTCCA 2891140 1.2003238 15.414899 70-79
GTGGT 4201315 1.1925069 10.047459 50-59
AGAGC 2736005 1.1848165 7.1709733 140-149
TTGGC 3715460 1.1804234 7.8723364 60-69
AGCTT 2688165 1.1765797 6.3657503 150-159
GCATA 2297175 1.1738536 7.8125577 210-219
TGGTC 3672495 1.1667733 11.440291 50-59
TTCCA 2381605 1.1667687 7.3674064 230-239
TGCCC 3412290 1.1510248 6.4642873 60-69
ACTGT 2628000 1.150246 5.5545917 230-239
TATCA 1886745 1.1376765 6.6971316 70-79
AAAAG 1543170 1.1331273 13.571147 70-79
TCACG 2728590 1.1328374 9.302336 200-209
ATACC 1979885 1.132425 6.900238 70-79
GAAGA 2101310 1.1199919 17.94049 35-39
ATATC 1856610 1.1195055 7.1958165 70-79
TCTGT 2981635 1.1178033 6.356433 210-219
TCTAC 2277640 1.1158353 7.457278 180-189
CAATA 1567980 1.1038259 6.3652334 100-109
GTCTA 2516650 1.1015093 7.4667287 180-189
AGGTT 2811065 1.0992253 22.796707 10-14
GCTTC 3075255 1.0935936 5.6973796 150-159
AGTTC 2494185 1.0916768 5.234275 160-169
TGTAA 2024380 1.090556 6.7532153 230-239
ATCCA 1890595 1.0813543 14.991226 240-247
CCATA 1889875 1.0809425 9.628309 70-79
CTGAA 2114735 1.080627 14.076407 35-39
TACTG 2462215 1.0776839 6.586621 180-189
CGGCT 3568440 1.0753938 7.739751 130-139
CTCGG 3550725 1.0700552 8.291632 130-139
GAGCT 2880250 1.0683409 6.4010053 140-149
TATGT 2307655 1.0648106 7.353851 220-229
CTGCG 3494600 1.0531412 6.7986465 210-219
CTTCT 2493465 1.0463183 5.420195 210-219
GGGGT 4346350 1.0454774 7.9126596 10-14
CTACC 2243205 1.0424323 8.459036 230-239
AGAGA 1953310 1.0411084 12.46135 35-39
CTGCC 3085325 1.0407338 5.7677464 200-209
GTCCG 3447185 1.0388521 5.130148 130-139
AAAAT 1196460 1.0366898 5.260111 8
TAGAT 1923125 1.0360088 7.9036155 240-247
GCGGT 3824970 1.0298349 5.8382506 130-139
AGAAA 1400125 1.0280913 5.0168204 6
AACTT 1693140 1.0209358 5.7632375 200-209
CATGG 2720855 1.0092183 5.2667527 190-199
ATGGG 3030485 1.004251 29.090837 15-19
TGCGT 3125005 0.99283236 8.036809 210-219
TATAA 1333730 0.98983777 6.32307 240-247
ATACG 1929995 0.98622507 5.0695333 120-129
AAGAG 1832145 0.9765278 13.347028 35-39
CAGCG 2775405 0.97649497 6.262849 160-169
TCCCC 2560910 0.96690255 5.073695 80-89
CGAAC 1981000 0.9602146 13.145705 70-79
ACCCA 1767300 0.95883447 12.519452 240-247
CGGAG 3013720 0.9473205 6.457122 210-219
ACAAT 1345240 0.94702137 7.449694 100-109
AGCTG 2550380 0.9459858 5.0836835 90-99
TTCTG 2478020 0.9290001 5.3629146 210-219
ACTAA 1315630 0.9261766 11.915816 240-247
TAGGC 2489180 0.9232855 5.6946955 160-169
CCATT 1878790 0.9204353 5.7258873 50-59
CCCAA 1683180 0.91319597 9.558469 240-247
CGTGG 3375475 0.9088128 7.03851 50-59
TGCAT 2058075 0.9007963 6.0405335 210-219
TGAAC 1761480 0.9001142 6.3871365 190-199
TCGAA 1750635 0.8945723 16.69529 70-79
ACGTC 2121450 0.88076913 10.631956 70-79
GTATG 2245800 0.8781868 5.1514063 220-229
CATCA 1530035 0.87512654 5.578425 230-239
TGAAG 1911730 0.87276405 9.203383 35-39
CCAGC 2197985 0.86560136 5.7765536 80-89
CCAAT 1502225 0.85922027 10.458437 240-247
GGTGG 3559995 0.8563264 20.371235 25-29
CGGTC 2817875 0.84920174 5.639899 130-139
AGCGT 2285205 0.84762716 6.425588 160-169
ATCAA 1200490 0.84512043 12.532706 70-79
CTTCC 2102875 0.837024 5.252928 230-239
AAAGA 1132385 0.8314938 5.7011175 8
GAGGC 2643590 0.83097535 12.996565 30-34
GGGCC 3089540 0.7890368 15.364174 15-19
TAACC 1376085 0.7870724 6.099018 80-89
GACCG 2207260 0.7765996 16.053658 45-49
AACCG 1596150 0.7736732 5.5886264 190-199
TGAGA 1684860 0.76919085 5.3958516 240-247
CGTCC 2255950 0.7609712 8.781901 70-79
AAAGG 1415485 0.75444925 8.639816 10-14
AGCCA 1545845 0.7492897 5.969525 80-89
ATGAT 1384055 0.7456059 6.8759565 240-247
CTAAT 1232090 0.7429302 9.252873 240-247
TGGGC 2726475 0.73407614 16.696796 15-19
AATCC 1283340 0.7340256 9.327689 240-247
GGTCC 2422675 0.73010325 11.723105 50-59
GTCGA 1957310 0.7260044 12.055516 70-79
GTAGC 1895900 0.7032263 5.5190353 80-89
AAGGT 1528525 0.6978191 9.694791 10-14
GGAAG 1793185 0.6937607 5.954855 30-34
CATAC 1202195 0.6876135 8.245521 70-79
AAAGC 1130335 0.67434406 9.392186 70-79
CATTG 1509710 0.6607831 5.189123 50-59
TATGA 1222880 0.6587791 5.6880145 240-247
TAGGT 1683545 0.6583253 5.239843 10-14
GAAAA 892545 0.65538275 5.9242287 8
CAATC 1130375 0.6465349 9.021157 240-247
GAGGT 1927490 0.63873726 6.7845945 10-14
AGGAT 1391345 0.63519216 5.3932486 10-14
AGAAG 1174520 0.62601566 5.1107693 9
ATAGG 1345485 0.6142556 5.1485667 10-14
GGGTC 2246485 0.60484356 5.865702 70-79
TAAAA 693880 0.6012221 6.064912 8
AAGAA 808600 0.5937432 5.139183 9
CCGTA 1428800 0.5931995 5.0020084 80-89
CGATG 1592220 0.59058553 13.0192375 15-19
AATGG 1275275 0.58220255 6.4488735 10-14
AAATG 906620 0.5702112 5.013204 9
CATCC 1224400 0.5689869 7.1589756 240-247
GAAGG 1413455 0.54684794 5.443887 10-14
AAGGG 1348610 0.5217602 6.2681484 10-14
AAGCA 868245 0.5179844 6.181914 70-79
GGTCG 1860400 0.50089407 5.930571 70-79
TGATC 1138070 0.49812046 5.2460017 240-247
GATCC 1150035 0.4774637 5.3379993 240-247
ATAAG 750405 0.471961 5.4287696 9
CGGTT 1417550 0.45036393 8.072693 10-14
ACGAT 879290 0.4493161 5.7237077 10-14
TAAGG 970470 0.4430496 5.668937 10-14
TAAAG 649205 0.40831208 5.4505186 9
TGGGT 1405000 0.3987971 5.0724163 10-14
AAGCT 774610 0.3958248 7.395774 30-34
Produced by FastQC (version 0.10.1)