FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_9_SL248355.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_9_SL248355.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12738936
Filtered Sequences 0
Sequence length 251
%GC 54

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 53242 0.41794699337527086 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 46228 0.3628874499408742 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 43783 0.34369432423555624 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 43444 0.34103319146905203 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 40407 0.3171928958588064 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 39436 0.30957059522082536 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 38245 0.3002213057668238 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 37136 0.29151571214424815 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 34824 0.2733666296777062 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 34221 0.26863311033197745 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33650 0.2641507893594881 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 33401 0.26219615201771956 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33386 0.26207840278026356 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 32316 0.2536789571750733 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 32019 0.25134752227344576 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 31446 0.2468495014026289 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 31150 0.24452591645016505 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 30377 0.23845790574660236 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 30340 0.23816745762754438 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30058 0.23595377196337278 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29750 0.23353598762094419 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29553 0.23198954763568952 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29177 0.22903796675012733 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 28671 0.22506589247328035 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27888 0.2189193822780804 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27683 0.21731014269951587 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27547 0.2162425496132487 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26189 0.20558231864890442 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25753 0.20215974081351848 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25508 0.20023650326840484 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25492 0.20011090408178517 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24682 0.19375244525916452 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24220 0.1901257687455216 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23837 0.1871192382158133 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23825 0.18702503882584856 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 23681 0.18589464614627155 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 23380 0.18353181144798908 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22530 0.17685935465881922 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22465 0.17634910796317682 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22443 0.17617640908157478 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 22365 0.1755641130468039 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21997 0.17267533175455158 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21845 0.1714821394816647 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21308 0.16726671678074212 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 20942 0.16439363538681723 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 20864 0.16378133935204636 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20781 0.16312979357145685 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 20653 0.1621250000784995 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20201 0.1585768230564939 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 19851 0.15582934084918867 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 19626 0.1540631022873496 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19513 0.1531760580318482 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 19160 0.15040502597705177 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18976 0.1489606353309256 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 18233 0.1431281231022748 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18076 0.14189568108356929 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18009 0.14136973448959944 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 17665 0.1386693519772766 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 17504 0.1374055101619162 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17327 0.1360160691599361 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16272 0.1277343727922018 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15473 0.12146226341038216 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15200 0.11931922728868408 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15103 0.11855778221980234 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14875 0.11676799381047209 No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG 13764 0.10804670028956892 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12931 0.10150769263618249 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 25703465 7.617062 66.50326 35-39
TATTA 12721955 7.25542 126.03361 240-247
TGGAT 19524030 7.165098 79.351555 35-39
TCACC 19889670 6.4121857 74.72442 160-169
TCTCC 17714580 6.131258 87.2588 20-24
CTGTG 18624260 5.9253654 87.18656 25-29
TCCCT 16664305 5.767744 72.20178 15-19
AGTGG 19982335 5.677416 39.474678 100-109
CTGAG 19015135 5.6350174 50.26077 15-19
TGTAT 11623745 5.50994 59.041996 240-247
TTACT 10684090 5.28238 111.81368 240-247
ATTAC 10815910 4.980982 104.61835 240-247
TGGGT 16127395 4.919364 45.171192 100-109
CCTGA 15749300 4.8679757 51.908066 15-19
CTCTG 14231685 4.722628 73.27576 35-39
TTCAC 11719205 4.678789 74.68345 40-44
CTCCA 14242550 4.5916233 41.206448 80-89
GACAC 15875615 4.570645 46.17323 230-239
ACACG 15826325 4.556454 48.75979 230-239
TGAGA 13010155 4.447284 93.7709 15-19
ATTCA 9420065 4.338162 111.537285 40-44
TCTGG 13542340 4.3085365 134.47517 35-39
TACTG 11182700 4.2804623 78.96415 240-247
AAGGG 16147725 4.2734203 38.955414 90-99
AGCCT 13525270 4.1805468 51.2455 30-34
CCCTG 15557495 4.1687922 84.85266 10-14
CAGCC 16608015 4.145225 55.534477 30-34
TGGAG 14530700 4.1284885 38.540165 90-99
GAAGG 15576190 4.1221657 30.825645 150-159
CCAGG 17164885 4.1075225 52.883636 80-89
ACCAT 10937060 4.067201 67.30964 160-169
GAGAC 14522640 4.0086775 84.843094 8
TCAGT 10448920 3.9995894 97.69781 7
TCCAG 12885635 3.9828413 40.581448 80-89
CACCA 13152935 3.9496713 57.270985 160-169
GTATT 8294050 3.9315832 68.402756 240-247
GGGTC 15951445 3.9290535 125.07696 8
ACTAC 10486025 3.8994734 35.03665 130-139
TACTA 8320560 3.8318143 28.413612 130-139
CTGGG 15452495 3.8061557 132.89778 3
CACCT 11715335 3.7768803 44.10046 40-44
GACTC 12143355 3.7534087 52.128937 20-24
ACTGT 9767110 3.7386093 67.91988 240-247
GATTC 9730445 3.7245743 58.973858 40-44
TGAAG 10885580 3.7210371 39.406025 150-159
AGACT 10358780 3.6932807 68.82642 15-19
GGACA 13139825 3.6269803 28.175259 220-229
CAAGA 10743640 3.5679188 43.61322 180-189
GCTGG 14417135 3.5511327 33.64866 2
ACTGG 11870100 3.5176306 44.06543 60-69
CCTGT 10568140 3.5069208 62.285023 25-29
ACTCT 8695335 3.4715362 68.68758 20-24
GAGTG 11967775 3.400305 38.98135 90-99
TCCTG 10201140 3.385136 64.63311 20-24
CTCCT 9570960 3.3126402 94.70462 20-24
TATCT 6690075 3.307677 48.657257 200-209
GGATT 8844325 3.245767 56.393227 40-44
GGTCC 12614100 3.2406754 141.65051 9
AGACA 9725150 3.229682 37.42886 170-179
AGCTG 10885880 3.2259626 36.610397 60-69
CTGCA 10417335 3.2199104 44.5318 25-29
CATCT 7963000 3.1791577 33.05036 170-179
GTCTC 9419605 3.1257923 32.688465 30-34
CCATC 9668925 3.117143 34.19271 45-49
GTCCC 11374125 3.0478144 75.13607 10-14
CCTCA 9304950 2.9998016 82.77614 5
GGAGT 10424545 2.9618402 36.93061 90-99
GGCTG 11888145 2.9282086 34.194275 90-99
AAGAA 7557600 2.895103 36.87022 180-189
AGGGA 10852390 2.872034 39.95504 80-89
AGTAG 8254760 2.821739 28.110754 50-59
TGTGC 8860750 2.8190744 57.433594 25-29
GAGCT 9509080 2.8179567 29.401615 60-69
ATCTC 7045065 2.81268 48.11487 170-179
GTGAA 8166940 2.7917194 39.51317 10-14
GTGTA 7589690 2.7853303 36.21594 230-239
ATCTG 7256780 2.7777166 36.556274 200-209
GGAAG 10366570 2.7434642 29.887445 80-89
GACAA 8260080 2.7431383 24.741175 180-189
CAGGG 11937625 2.7388334 36.065388 80-89
ACCTT 6758425 2.6982415 72.024704 40-44
CTATG 7025395 2.6891484 27.758562 50-59
GAACA 8019590 2.6632726 34.607265 210-219
ATGAA 6337005 2.60618 27.39789 210-219
GGGAA 9835580 2.6029403 33.518894 80-89
CACGG 10714610 2.5639844 44.016605 230-239
GAGTC 8614680 2.5529068 27.625761 150-159
TGCAA 7159600 2.5526571 35.532837 200-209
CGCCA 10206175 2.5473778 39.86749 70-79
AGGGC 11055695 2.5364933 32.948566 150-159
AGAAC 7557635 2.5098593 29.96119 190-199
CAGTG 8455670 2.5057852 75.59892 8
CCCTC 8942965 2.4994442 26.910583 20-24
AGGCT 8338785 2.471147 20.699205 25-29
ATCAG 6918280 2.4666178 26.221327 45-49
CTACA 6605710 2.456488 19.95838 190-199
CCAAG 8525720 2.4545846 38.36757 180-189
ACGGC 10185815 2.437445 48.484955 230-239
TATAT 4270505 2.435499 23.996122 240-247
GTGGA 8504915 2.4164314 26.259449 100-109
TCTGT 5832065 2.3966608 24.227507 210-219
CACTG 7727550 2.3885205 19.482862 60-69
CTCTC 6852320 2.3716817 98.131676 20-24
GCAGC 9871320 2.3621867 34.285793 30-34
CCGCC 10828435 2.3430386 34.126965 70-79
TGAAC 6559210 2.3385966 37.579494 210-219
ACAGC 8121025 2.3380716 26.740097 210-219
CTGTC 7041680 2.336704 47.91403 30-34
TATGC 6068510 2.3228762 21.238178 50-59
CTACT 5814530 2.3214 22.71985 50-59
CTTCA 5810785 2.3199043 50.606945 45-49
GGGCT 9367415 2.3073192 42.398857 90-99
GTATC 6012705 2.3015153 22.76134 200-209
CAAAT 5347105 2.293667 35.09454 200-209
GCCTC 8439760 2.2615213 73.02177 4
CAGGC 9450100 2.2613897 33.563297 70-79
GTGGT 7406000 2.2590635 30.88559 120-129
GGCCG 11336950 2.2549002 24.96105 230-239
TCCAA 6057170 2.2525003 42.109646 180-189
TGGTG 7333000 2.2367961 33.591908 35-39
GCCTG 8593245 2.2076817 31.17455 210-219
ACAAC 6335730 2.1945803 20.07812 180-189
AAATG 5333160 2.1933348 33.285988 200-209
GCTCC 8179710 2.1918383 33.966797 80-89
AATGA 5321190 2.1884122 25.862225 210-219
TATCA 4722850 2.1749837 16.783985 170-179
TACAT 4696350 2.16278 18.817373 190-199
GGGGG 11819755 2.1610107 94.664215 5
CCTTC 6211970 2.1500478 55.350574 45-49
CAGTA 6007110 2.141753 20.983213 45-49
GCTAT 5498285 2.1046083 33.955257 50-59
CCTCT 6068590 2.1004221 49.14322 35-39
GTGGG 8886280 2.0985367 29.906853 100-109
TGCAG 7044835 2.087693 43.45378 30-34
GTAGT 5644200 2.0713575 24.656887 120-129
GCAGA 7483680 2.0657167 24.07326 140-149
GCTGT 6486955 2.0638447 19.40985 230-239
TCTAT 4135805 2.0448065 19.546492 110-119
AACAG 6089170 2.0221884 33.25403 210-219
TGCAC 6527525 2.0176027 25.879234 25-29
AACTA 4683035 2.008811 36.642303 130-139
CAGAG 7263575 2.0049615 32.32926 170-179
CTCAC 6216925 2.0042603 54.19107 20-24
ATGGA 5835120 1.9946294 12.450486 100-109
AGAGT 5819975 1.9894521 30.304419 150-159
TGTGA 5381440 1.9749273 18.54935 210-219
AGCAC 6852380 1.9728241 20.277855 130-139
TGAGC 6606835 1.9578946 17.849333 200-209
TGGTA 5332710 1.9570442 30.955318 120-129
GGCTC 7610350 1.9551672 19.572407 40-44
TCCGC 7283310 1.9516385 42.066162 70-79
TTCAG 5080380 1.9446443 47.205727 45-49
GGTCT 6102030 1.9413795 36.473022 15-19
TCTCT 4509535 1.9328909 32.434277 35-39
ATATC 4183945 1.9268055 13.431032 160-169
CCGTG 7476620 1.9208107 20.090252 230-239
CTGTA 5009015 1.9173276 24.065033 190-199
CATAT 4138500 1.905877 14.221595 160-169
CAGAC 6615685 1.904679 26.229988 140-149
GCACA 6611255 1.9034034 18.530315 130-139
ACTAT 4102360 1.8892337 17.370926 130-139
TGGGG 7982900 1.8851993 123.3865 4
GCCAG 7870520 1.8833995 35.495785 70-79
GGTGG 7972815 1.8828176 17.945702 40-44
CCAGA 6531530 1.8804502 33.936844 170-179
GGATC 6325855 1.8746278 17.872925 70-79
GATGG 6579025 1.869244 21.34336 100-109
GTCAC 6014720 1.859099 38.05394 160-169
CCTGC 6925840 1.8558507 49.957676 20-24
AGGAC 6712070 1.8527294 20.74435 220-229
ACCCT 5746135 1.8524834 42.89861 10-14
TCCAT 4639515 1.8522856 26.191912 45-49
ACCAG 6398575 1.842172 13.667547 190-199
AGAGA 5782415 1.8411169 27.969292 170-179
CTATT 3714800 1.8366551 19.213547 110-119
CACAG 6373270 1.8348867 16.207664 140-149
TTCCA 4575295 1.826646 22.688349 180-189
TGGGA 6389310 1.815342 16.97437 100-109
CAGTT 4738040 1.8136052 24.126913 190-199
GCTGA 6118645 1.8132225 26.948929 200-209
TCAGC 5848665 1.8077731 11.520657 45-49
CAAGG 6516745 1.7988143 17.545958 80-89
GAGGA 6793150 1.7977753 27.014534 220-229
GCCGA 7494070 1.7933158 19.223766 220-229
TGTCT 4356435 1.7902573 48.86081 30-34
ATACT 3858755 1.7770478 26.411615 130-139
TTCTG 4274610 1.7566317 43.672157 30-34
TAGTG 4762870 1.7479193 25.851028 120-129
TAGTA 3949340 1.7437533 16.78293 120-129
AGTCA 4887235 1.7424766 28.645035 160-169
ACCTG 5609110 1.7337286 30.248789 25-29
GCAAA 5217590 1.73274 32.069206 200-209
AAAAA 3755680 1.7309183 10.1736965 90-99
CCTGG 6726305 1.7280481 33.839554 2
AGTTC 4482640 1.715844 21.41307 190-199
CATGA 4812355 1.7157793 23.032125 60-69
TACAA 3998705 1.7152642 17.415205 130-139
CTCAG 5526495 1.7081931 79.06863 6
TGACC 5494585 1.6983299 14.163444 210-219
ATGCA 4761710 1.6977223 16.553158 60-69
CTGAA 4753800 1.6949023 22.694195 200-209
GTGCA 5713695 1.693218 50.276333 25-29
GGATG 5944445 1.6889464 22.772457 100-109
AGCTA 4692340 1.6729894 25.578182 50-59
GGAGC 7235755 1.6600896 13.332041 200-209
AACAC 4774590 1.6538302 22.152193 190-199
ATTGG 4434940 1.6275728 20.146784 100-109
GCCGT 6305260 1.6198778 29.10236 230-239
TCTGC 4853085 1.6104428 33.436726 200-209
CGATT 4196905 1.6064719 35.468582 160-169
AGGGG 7302060 1.6062077 32.661983 90-99
TCTCA 4006255 1.5994618 26.08178 100-109
CAGCT 5170495 1.5981566 12.850779 50-59
TCTGA 4174790 1.5980068 21.634731 220-229
GGGCC 8024625 1.5960844 28.553432 2
GGGGC 8296615 1.5821246 28.52733 90-99
CTCCC 5528240 1.5450724 13.857243 190-199
GGCTT 4853140 1.5440413 21.943249 25-29
ACAAT 3593010 1.5412394 22.789085 180-189
GCACT 4963030 1.5340308 24.836285 25-29
AGTGA 4471415 1.5284716 86.9232 9
CCATG 4918410 1.5202391 15.691005 160-169
GTTCT 3691145 1.5168594 22.583725 190-199
CGTCC 5654820 1.5152677 18.699987 180-189
GTCCG 5843305 1.5011973 34.46373 70-79
GAGCA 5436100 1.5005242 7.8561096 200-209
AGTTA 3397800 1.5002316 15.698206 50-59
CGGCC 7209350 1.4956107 31.333084 230-239
TTCTC 3475735 1.4897803 21.502388 190-199
TTAGT 3126485 1.4820306 25.317102 110-119
CTTTA 2972170 1.4694872 41.960346 45-49
CATCA 3931845 1.4621485 30.509615 45-49
ACTCC 4482730 1.4451772 16.901077 140-149
GTTAC 3774290 1.4447052 15.555868 50-59
CTGGT 4539880 1.4443768 30.915508 35-39
TACGC 4622825 1.4288763 24.331446 140-149
GGGGT 5939160 1.4025605 117.63165 7
GTGAC 4715190 1.3973173 14.8273 210-219
GGCAG 6068105 1.3921973 13.827357 150-159
CGTGT 4350065 1.3839866 23.863625 230-239
CTCAA 3708990 1.3792747 18.289843 150-159
GGCCT 5368495 1.3792145 69.780426 3
GTCCA 4459130 1.3782793 28.425156 180-189
TAGCA 3841290 1.3695592 11.557037 130-139
CAACT 3674850 1.3665788 17.372513 130-139
CAACC 4537565 1.3625773 14.993462 140-149
CGACA 4730985 1.3620671 16.532448 70-79
CTACG 4389795 1.3568486 16.23355 130-139
AAGAG 4251055 1.3535331 18.436876 150-159
AACCC 4500810 1.3515402 19.771511 140-149
GAACT 3786195 1.3499159 14.63648 190-199
AAGCT 3779110 1.34739 16.279474 200-209
AGCAG 4881105 1.3473291 12.170613 50-59
GAGAG 5085465 1.3458444 25.535334 220-229
TGGAA 3905120 1.3348941 19.35791 120-129
ATACA 3111245 1.3345839 22.208658 35-39
ACAGT 3699640 1.3190562 12.254555 120-129
ACAGA 3971380 1.318879 20.440916 140-149
GTGCG 5352345 1.3183539 17.111395 240-247
ATCTA 2853110 1.3139244 20.138016 110-119
ACATG 3680255 1.3121445 14.784378 190-199
TTATT 2133685 1.3064092 20.515194 240-247
CCTTT 3047550 1.3062505 48.274902 45-49
TTACA 2836120 1.3061001 9.612077 120-129
CCATA 3497230 1.3005266 14.641551 160-169
TGTGT 3296415 1.2987775 15.017624 240-247
TCACT 3249400 1.2972944 13.431916 190-199
CACGT 4195235 1.2967118 16.324116 180-189
GTGGC 5259560 1.2954997 13.914315 40-44
CAATT 2811140 1.2945964 23.790493 180-189
TCAAG 3607555 1.2862244 22.677158 150-159
AGAGC 4652665 1.2842729 24.426874 220-229
TGCGA 4321215 1.2805651 14.16406 240-247
TGTCC 3856505 1.2797387 53.136074 15-19
TCCGT 3835555 1.2727867 13.651132 150-159
AATTC 2752065 1.267391 23.818361 180-189
CCTAC 3919400 1.2635665 17.609652 190-199
ACATA 2944910 1.2632337 20.735764 130-139
ATATT 2204950 1.2574984 25.675848 240-247
ATTAT 2204545 1.2572675 9.894493 50-59
AACCA 3628545 1.2568612 17.107714 190-199
ACACA 3617160 1.2529176 7.9348526 130-139
ATCAT 2716125 1.2508396 15.812903 110-119
CCAGT 4034755 1.2471088 18.458157 190-199
CTTCT 2891880 1.2395266 23.882864 30-34
AGACC 4285635 1.2338493 97.84078 9
CGGCT 4791540 1.2309896 21.308086 230-239
TGGAC 4098290 1.2145028 16.123407 80-89
ACGTC 3923210 1.2126312 18.114504 180-189
TGAGT 3298420 1.2104828 21.261244 90-99
ATCCG 3912685 1.209378 23.353695 70-79
GATTG 3292105 1.2081652 23.234583 100-109
GATCT 3145940 1.2041882 9.548367 220-229
AGTCG 4051760 1.2007139 15.711859 150-159
TACCA 3219025 1.1970696 11.015993 45-49
TCGGA 3997930 1.1847618 91.25188 5
AAGGC 4267530 1.1779644 29.095833 25-29
GGGTG 4968095 1.173239 14.825436 60-69
GAAGT 3396710 1.1611032 13.726411 140-149
GTCGA 3916175 1.160534 12.24561 150-159
ATTAG 2627560 1.1601474 25.020742 110-119
AGATC 3244805 1.1568906 12.857508 210-219
AGGCC 4829855 1.1557744 13.9109 2
ACGCA 4000985 1.1518977 20.760603 140-149
GATCC 3714395 1.1480881 23.210396 70-79
ATTCC 2869385 1.1455766 22.132454 180-189
CTTCG 3450135 1.1448894 103.03465 3
CGCTG 4445795 1.1421646 12.126991 190-199
GCAAG 4088345 1.128504 17.224543 25-29
ACAAG 3386845 1.1247573 17.67348 80-89
GGACT 3794780 1.1245595 12.757749 90-99
AGCTC 3636825 1.124112 12.116031 210-219
AGGTC 3785415 1.1217841 18.187717 10-14
TCGAG 3756005 1.1130688 11.942705 150-159
GCCCC 5119230 1.1076905 12.237631 80-89
TTGGG 3625060 1.1057576 14.991643 100-109
CTTGA 2880055 1.1024141 22.593948 90-99
GACCC 4414780 1.101893 42.112385 10-14
GCTTC 3320125 1.1017469 27.515184 2
GAACC 3826325 1.1016123 10.342178 180-189
CCGAT 3559675 1.1002655 23.722452 160-169
TTGAG 2991085 1.0976943 20.9725 90-99
GTATA 2475490 1.0930039 10.089285 240-247
CCCCT 3903470 1.0909699 9.92241 70-79
CACAT 2929860 1.0895369 13.769953 130-139
TTCGG 3401975 1.0823486 98.68387 4
ATGGG 3802020 1.0802367 20.279888 100-109
CGCAG 4513445 1.0800582 16.35943 140-149
GCACC 4299640 1.0731549 10.920549 130-139
ATCAA 2501275 1.0729342 12.674997 110-119
ACACC 3558035 1.0684359 13.31736 40-44
TACAG 2990390 1.0661825 9.091272 120-129
GAAGC 3845960 1.0615985 12.700677 200-209
TAGAC 2951710 1.0523918 16.953814 170-179
CAGCA 3650095 1.0508752 12.5220995 45-49
ATGGT 2857020 1.048494 9.594763 35-39
CTCCG 3890585 1.0425227 9.803632 140-149
CTATA 2255650 1.0387801 13.309363 50-59
ATGAG 3032040 1.0364476 19.842173 60-69
GACAG 3749500 1.0349728 15.886023 70-79
GGAGA 3895535 1.0309352 81.24234 7
GAGCC 4290980 1.0268227 17.86112 220-229
TGGCT 3227400 1.0268072 30.511932 40-44
AAACT 2364315 1.0141846 17.27779 130-139
CGTGA 3421895 1.0140574 20.28445 150-159
GGGAG 4609275 1.0138856 14.5777025 5
TGAGG 3567510 1.0136073 12.076606 220-229
GCTCT 3051690 1.0126697 16.927746 210-219
GTAGA 2947815 1.0076568 16.615435 170-179
GTAGC 3396185 1.0064384 11.183198 50-59
ACCGC 4026455 1.0049702 10.910686 220-229
CGAGG 4357330 0.9996964 14.536636 220-229
TAGTT 2105520 0.99806815 14.552639 50-59
TTTCA 2017850 0.9976564 7.2865653 100-109
AGCCG 4168330 0.99747294 18.876131 220-229
ATGAC 2790780 0.99501437 11.088389 160-169
ACTCA 2674345 0.99451774 12.340648 190-199
AAGGT 2896610 0.9901533 21.045229 15-19
AACTC 2651810 0.9861375 16.437893 190-199
GCCAA 3416280 0.9835589 14.91694 180-189
TTATA 1715190 0.9781849 13.123208 110-119
ATGAT 2188745 0.9663974 8.91564 120-129
ACCAA 2788800 0.965989 16.735086 130-139
GGTAT 2631960 0.9658995 9.367791 110-119
GATAC 2706065 0.9648103 24.10595 35-39
CCGTC 3600455 0.9647793 10.361123 140-149
TTTAG 2016195 0.9557258 29.489912 45-49
CCGAG 3976240 0.9515062 17.751844 220-229
CGAGT 3210275 0.95134497 14.714938 160-169
CCAAC 3159980 0.94890475 13.876501 130-139
ACAGG 3430835 0.9470119 15.443821 70-79
CAGAA 2849840 0.9464202 18.36929 140-149
GGGAC 4098255 0.9402572 9.795787 90-99
GCAGT 3172610 0.9401832 6.009818 50-59
GCCAT 3037235 0.93878376 7.69994 60-69
AACTG 2632100 0.9384392 11.880218 60-69
TACAC 2516425 0.93579143 22.411692 35-39
CGGAC 3906025 0.9347039 10.70662 220-229
TTTAT 1523345 0.9327112 10.867442 240-247
CATAC 2506380 0.93205595 13.981439 130-139
TATGG 2521260 0.9252739 12.410134 35-39
TAACA 2155515 0.9246187 8.745991 120-129
AATCA 2155040 0.924415 13.987572 110-119
AGCCC 3681805 0.9189484 11.22626 80-89
GAGAT 2673940 0.9140375 12.337486 210-219
AAGTT 2067505 0.91286623 17.756739 140-149
TGGTT 2306160 0.9086202 9.211233 50-59
CCCAG 3597515 0.8979103 14.552572 80-89
TCATA 1949125 0.8976181 8.708233 110-119
CGTAT 2339115 0.89535564 7.422262 240-247
CCCCA 3435420 0.89433765 16.67799 80-89
TCCTT 2085575 0.89392567 10.854135 45-49
TATTT 1448375 0.88680875 10.867821 240-247
GTTAT 1863790 0.88348216 10.710571 110-119
AGAAG 2768705 0.88155377 16.06227 140-149
GACTG 2947545 0.8734866 12.400962 90-99
AGTCT 2271850 0.8696081 15.8008585 8
GATGA 2538090 0.8675997 6.744578 60-69
TTACC 2162870 0.8635067 10.203181 45-49
CGGAG 3750110 0.86038274 70.61978 6
GATCA 2406380 0.8579618 9.4699545 110-119
ATCCA 2306695 0.8577984 10.019885 180-189
GCCTA 2765170 0.85469073 16.870747 190-199
GTTTC 2078705 0.85423446 5.036838 100-109
CATGG 2874425 0.851818 8.782745 200-209
GTCTG 2672105 0.8501383 14.43339 3
ACGCT 2737765 0.8462202 14.878879 190-199
ATAGT 1913270 0.8447668 15.393134 120-129
CTATC 2098540 0.8378237 8.363919 110-119
CATTA 1819010 0.8376971 12.209156 110-119
GGTAG 2945045 0.83675146 11.826241 120-129
GCTTG 2614195 0.83171415 17.313854 90-99
TCATC 2081540 0.83103657 10.675929 110-119
GTCTA 2169590 0.8304656 10.019372 30-34
TCTTC 1933495 0.8287406 44.53213 2
GGTTC 2602520 0.8279997 11.236781 160-169
GGTGC 3343090 0.8234476 11.905056 60-69
ACAAA 2059470 0.8228618 12.986259 130-139
TGGCA 2758560 0.8174821 5.6991353 100-109
TTATG 1711500 0.811293 11.57495 50-59
ACATC 2174490 0.8086349 5.5756526 110-119
ATATG 1823690 0.8052145 5.731069 50-59
ATATA 1505815 0.79990816 10.519604 130-139
CCTCC 2844480 0.7949958 10.328278 140-149
TGATG 2145710 0.78745127 11.928047 120-129
TATAC 1649015 0.7594103 8.828669 130-139
CTGAC 2453785 0.75844425 9.678109 220-229
GGGAT 2666895 0.7577229 13.639551 100-109
CCAGC 3028535 0.7558976 6.2072477 80-89
GTTCA 1973900 0.7555603 13.583166 160-169
CATCC 2323620 0.7491066 6.7852864 110-119
TCAAC 2014175 0.749018 7.6534457 110-119
GGTTA 2019145 0.74100333 8.129104 50-59
CCCCC 3263910 0.73661965 13.243867 80-89
TTTAC 1488210 0.7357941 13.805709 45-49
GATTA 1661615 0.73365337 5.7797093 50-59
ATCTT 1482515 0.7329785 11.876837 3
TTGGA 1991510 0.73086154 6.4011755 60-69
ATAAA 1470900 0.7277989 7.7083545 130-139
ACGCC 2906910 0.725541 13.098554 180-189
AACGC 2515550 0.72423565 14.536763 180-189
GCGGA 3144505 0.7214396 8.927128 220-229
TATTG 1516790 0.7189957 6.527911 240-247
GGGCA 3126080 0.7172124 13.8273 150-159
CGCCG 3447070 0.71510947 10.725678 220-229
ATGCC 2293845 0.70900816 8.037729 50-59
GACCG 2961055 0.7085745 9.138413 210-219
GACCA 2456725 0.7072997 6.2729573 170-179
CAACG 2452205 0.70599836 14.819237 180-189
GGATA 2063950 0.70552367 15.844888 35-39
TTTCT 1323875 0.70271516 9.385057 4
CACAA 2027725 0.70236665 8.430468 130-139
AATAC 1633820 0.7008351 11.322378 130-139
TCCGG 2725355 0.70016813 11.413034 70-79
TGCCA 2265050 0.7001078 7.3864956 60-69
CAAAC 2008940 0.69585985 13.974765 130-139
AAATA 1395110 0.69029814 12.570691 130-139
GAAAT 1671440 0.6874024 7.26963 110-119
TATAG 1532105 0.6764708 6.18757 120-129
CAGTC 2187255 0.67606217 5.4700227 210-219
AGGCA 2415580 0.6667715 6.546489 80-89
GCGAC 2758420 0.6600843 7.345616 70-79
CCCGT 2446900 0.65567225 10.10675 140-149
GCCGC 3146500 0.65275496 11.760431 220-229
TCCAC 2023160 0.65224195 10.592193 180-189
GACGA 2358195 0.6509315 9.895268 220-229
AATAA 1314110 0.65021944 7.194024 130-139
AGATG 1892040 0.64675933 5.836028 120-129
CACGC 2585285 0.645266 12.054256 190-199
ATTAA 1195085 0.63484436 6.4585466 110-119
ACTTC 1579110 0.6304458 29.585447 4
TCAAT 1365110 0.6286654 8.469655 110-119
CACCG 2515425 0.6278295 9.1288395 45-49
TACTT 1260880 0.6233987 11.007533 1
AAGCA 1861550 0.6182131 5.919947 120-129
CACGA 2142525 0.6168404 9.011181 180-189
CCCTA 1912460 0.61655366 12.018663 110-119
GGCCC 2945820 0.611123 10.555845 70-79
CCGGC 2933440 0.6085547 9.262334 70-79
AATGG 1780235 0.6085409 6.830659 120-129
CCGTA 1952590 0.6035291 6.2039676 230-239
TAGCT 1575250 0.6029669 12.344134 50-59
CTTTG 1454400 0.59767914 7.43362 45-49
ACGAC 2065370 0.59462726 9.244993 220-229
TGATT 1254135 0.59449077 6.238727 50-59
AGTAA 1445305 0.59440136 5.1753163 50-59
GGTGA 2062340 0.5859557 6.5148106 120-129
GGGGA 2653750 0.5837358 9.245253 4
GTTCC 1754155 0.582097 6.9750466 140-149
GAAGA 1821340 0.5799134 6.5120673 15-19
ATTTC 1154115 0.57061243 6.482813 240-247
ACCCG 2285825 0.5705232 8.61303 140-149
ACCTA 1511940 0.5622502 6.923232 130-139
TAATA 1057865 0.5619514 7.871596 130-139
TCAGG 1891765 0.5606128 5.412473 150-159
TTCAA 1212715 0.5584839 5.460385 200-209
GTCCT 1676150 0.5562119 6.503397 45-49
TTAGC 1448295 0.55437165 9.317947 45-49
AAGAT 1345990 0.5535568 5.952209 15-19
CGGTT 1731150 0.55077064 11.127714 160-169
CCACG 2199815 0.5490558 7.9477034 180-189
CTGCG 2121510 0.54503495 11.965845 25-29
GGAGG 2477415 0.5449481 5.44891 6
CCTAT 1358270 0.5422774 5.213398 110-119
CGGCA 2260680 0.54097617 10.611379 70-79
GCTAC 1728880 0.5343822 7.9764986 50-59
CTGTT 1299730 0.53411824 5.635624 240-247
AAATC 1242515 0.5329829 7.884984 110-119
TATGT 1111340 0.5268024 6.7651825 140-149
ACATT 1111760 0.51199174 6.0918956 110-119
ACCCC 1962245 0.51082826 8.80945 140-149
GGGTT 1635265 0.49880737 5.823307 100-109
GTAAC 1384410 0.49359244 6.976064 120-129
TGACG 1661380 0.4923396 8.770283 220-229
TGGCC 1903445 0.4890121 15.790776 2
TATCC 1213940 0.48465478 5.5664625 110-119
GGGTA 1702315 0.48366472 5.0467873 100-109
AACAT 1124270 0.48226118 5.845721 110-119
CGCGG 2406155 0.47858018 6.3911095 220-229
CAATC 1255135 0.46675128 6.3824024 110-119
ATGCT 1210540 0.4633649 7.1435437 50-59
CATAA 1080040 0.4632885 5.9903383 110-119
GGTTT 1174515 0.4627554 5.398732 100-109
TAAAT 863540 0.45872346 7.739208 130-139
CCGGT 1777765 0.45672378 8.66873 160-169
TGCTA 1183750 0.45311034 6.3841844 50-59
ATTCT 901615 0.44577247 7.593585 2
CCGCG 2144015 0.44478512 6.627786 220-229
TGCGC 1716575 0.44100347 8.931213 25-29
TTGGT 1114005 0.4389146 5.336495 120-129
GCCTT 1310195 0.43477377 8.81175 1
GGTCA 1454720 0.43109724 5.569405 160-169
GCATG 1423250 0.42177126 8.348375 60-69
GCGCT 1629245 0.41856766 8.064976 25-29
CACTT 1038010 0.4144163 6.861178 3
ACGAG 1495895 0.41291124 7.867043 180-189
ATGGC 1372480 0.40672594 7.2874904 1
TACCT 1015660 0.4054933 5.288118 2
CAATG 1125730 0.4013636 5.081803 120-129
CTAAC 1077575 0.40072143 5.5363417 110-119
AGCTT 1046740 0.4006663 7.3788624 1
TTTGA 841175 0.39873758 6.249859 45-49
CATGC 1287310 0.39789668 7.060798 60-69
CCTAA 1061860 0.3948775 7.645823 110-119
CCGGG 1977165 0.3932548 5.2629724 80-89
GGTAA 1133085 0.38732445 7.3276577 120-129
GAGGC 1657665 0.38031584 5.9322915 35-39
TTCTT 677705 0.35972703 18.586958 1
CGAGC 1451380 0.34731224 6.7020473 180-189
TGCTG 1089580 0.34665316 14.174016 1
ATGTG 941770 0.34561893 5.0965953 140-149
TGGGC 1402280 0.34540024 9.767779 1
CTCGG 1290100 0.33143824 5.4098263 6
GCTTT 769645 0.31628218 5.2588153 45-49
CTCTT 730205 0.31298277 8.624025 1
GCGAG 1328365 0.30476502 5.428464 240-247
CGCTT 906525 0.30082035 5.3960795 1
AACTT 644745 0.2969203 10.018929 3
TCACA 788110 0.29307714 5.002574 5
GACTT 764335 0.29256862 6.4316435 3
TTCCT 676670 0.29003638 6.5889864 4
ATTGT 601210 0.28498828 5.3052044 240-247
GAGGT 981215 0.2787845 6.8563495 7
CCCTT 794750 0.27507386 5.4877415 3
TGTAA 605680 0.26742607 8.072142 25-29
TAACT 578360 0.26634842 6.8779497 2
TTGGC 825260 0.26255897 7.9563417 1
AATCT 562445 0.2590192 7.4501643 2
GTCTT 629095 0.25852376 12.078684 1
CGGGG 1348750 0.25720015 9.092738 3
TCGGT 785550 0.24992517 6.4569993 7
CCTCG 906830 0.24299452 5.920894 5
TGCTT 536950 0.22065718 11.044441 1
TCGCA 578385 0.17877392 6.0913644 5
TAGGC 535205 0.15860468 6.6002817 1
TTGCT 315585 0.12968823 5.8741283 4
Produced by FastQC (version 0.10.1)