FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_3_SL248349.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_3_SL248349.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9960256
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 49121 0.49317005506685774 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 40146 0.4030619293319369 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 37303 0.3745184862718388 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 34422 0.3455935269133645 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 32989 0.3312063465035437 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30813 0.309359518470208 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 30702 0.3082450892828458 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 30329 0.30450020561720503 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30325 0.3044600460068496 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29849 0.29968105237455744 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29280 0.29396834780150227 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 28835 0.28950059114946447 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 28005 0.2811674720007197 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27968 0.28079599560493224 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27544 0.2765390769072602 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26758 0.2686477134724248 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26408 0.2651337475663276 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26294 0.26398919867119885 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25244 0.25344730095290724 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24540 0.24637920953035747 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24444 0.24541537888182796 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24431 0.2452848601481729 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24217 0.24313632099415922 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23846 0.23941151713369616 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23524 0.23617866850008676 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23497 0.2359075911301878 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22824 0.22915073668789235 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21544 0.21629966137416545 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21492 0.21577758643954534 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21199 0.21283589498101255 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21137 0.21221342102050392 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21094 0.21178170520918335 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20979 0.21062711641146575 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20706 0.20788622300470994 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20195 0.202755832781808 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20001 0.20080809167957128 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 19447 0.19524598564534887 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19144 0.1922038951609276 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 19001 0.19076818909072218 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18706 0.18780641782701168 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18665 0.18739478182086886 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18412 0.18485468646589004 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 18379 0.18452336968045802 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 18334 0.1840715740639598 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17335 0.17404171137769953 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17143 0.17211405008064048 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 17143 0.17211405008064048 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 16988 0.17055786517936888 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16541 0.16607002872215332 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16514 0.1657989513522544 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 16467 0.1653270759305785 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16230 0.16294761901702123 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 16087 0.16151191294681583 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15616 0.15678311882746787 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15606 0.15668271980157938 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15423 0.15484541762782 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15400 0.15461449986827647 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14019 0.14074939439307582 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 13862 0.13917312968662654 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13840 0.13895225182967186 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13556 0.1361009194944387 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13371 0.1342435375155016 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12397 0.12446467239396256 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12382 0.12431407385512983 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12082 0.1213021030784751 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11499 0.11544883986917606 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11378 0.11423401165592531 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 11158 0.11202523308637852 No Hit
TAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 10383 0.10424430858002043 No Hit
AAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 10039 0.10079058208945633 No Hit
ATGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 9971 0.1001078687134146 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 20435000 7.746107 66.88995 35-39
TATTA 10151840 7.5084405 129.53436 240-247
TGGAT 15489715 7.3120737 80.26149 35-39
TCACC 15817340 6.4730415 75.589294 160-169
TCTCC 13992270 6.1192455 86.27655 20-24
CTGTG 14791065 5.9916034 87.5005 25-29
TCCCT 13374370 5.849019 73.16474 15-19
AGTGG 15958035 5.8217726 40.129627 100-109
CTGAG 15033275 5.6985254 50.42966 15-19
TGTAT 9207885 5.6244283 59.567196 240-247
TTACT 8604165 5.46085 114.991806 240-247
ATTAC 8636140 5.1290436 108.0219 240-247
TGGGT 12695035 4.9493084 46.10987 100-109
CCTGA 12466270 4.909965 51.925594 15-19
CTCTG 11301265 4.7566776 74.01281 35-39
TTCAC 9244630 4.71143 76.29393 40-44
ACACG 12705675 4.6827917 49.74663 230-239
GACAC 12618440 4.650641 46.676617 230-239
CTCCA 11324930 4.634581 41.39581 80-89
TGAGA 10238415 4.522672 95.69559 15-19
TACTG 8985210 4.407156 81.02309 240-247
ATTCA 7368290 4.376062 114.47376 40-44
AAGGG 12813220 4.3742065 39.856926 90-99
TCTGG 10642705 4.311175 136.42699 35-39
GAAGG 12363550 4.2206974 31.667728 90-99
TGGAG 11539840 4.209937 39.770615 90-99
AGCCT 10663725 4.200015 52.121513 30-34
CCCTG 12380325 4.184277 85.833565 10-14
CAGCC 13149810 4.158849 56.18553 30-34
ACCAT 8679565 4.139295 69.33786 160-169
CCAGG 13556715 4.1264353 53.718933 80-89
TCAGT 8357795 4.099415 98.79472 7
GAGAC 11512690 4.0836725 90.76652 8
ACTAC 8499260 4.0533075 36.925533 130-139
CACCA 10481525 4.013882 58.628166 160-169
GTATT 6547575 3.999438 69.676636 240-247
TCCAG 10148140 3.9969468 40.85089 80-89
TACTA 6712250 3.9864366 29.776098 130-139
GGGTC 12584920 3.9397876 127.24482 8
ACTGT 7879490 3.8648112 69.8291 240-247
CACCT 9314610 3.8118834 44.537434 40-44
TGAAG 8610875 3.8037298 40.572674 150-159
CTGGG 12111945 3.7917194 134.39209 3
GACTC 9548610 3.7608159 52.087147 20-24
AGACT 8149175 3.740323 70.039764 15-19
GATTC 7595730 3.72563 59.61011 40-44
CAAGA 8653815 3.7167919 45.74093 180-189
GGACA 10281560 3.646978 28.883926 220-229
GCTGG 11464425 3.5890093 33.648266 2
ACTGG 9444990 3.5802252 45.075474 60-69
CCTGT 8318670 3.50131 61.267754 25-29
ACTCT 6775730 3.4531808 68.70157 20-24
GAGTG 9329715 3.4036446 39.808952 90-99
TATCT 5327950 3.3815174 49.680534 200-209
AGACA 7783845 3.3431423 39.41475 170-179
GGATT 7081145 3.3427248 57.153854 40-44
TCCTG 7922550 3.334584 63.864994 20-24
AGCTG 8781640 3.3287756 38.18267 60-69
CTCCT 7456155 3.2608037 93.45693 20-24
CTGCA 8223470 3.2388961 46.411266 25-29
GGTCC 9863825 3.2084901 141.57121 9
CATCT 6207765 3.1637232 32.731472 170-179
GTCTC 7471585 3.144774 34.268696 30-34
CCATC 7611940 3.1150877 36.17179 45-49
CCTCA 7468400 3.056346 83.07926 5
GTCCC 9028790 3.0515323 74.6849 10-14
AAGAA 6033035 3.0196042 39.570076 180-189
GGAGT 8263860 3.0148017 38.118275 90-99
AGTAG 6754340 2.983632 29.825752 50-59
AGGGA 8591240 2.9328973 41.60388 80-89
GGCTG 9367075 2.932421 34.63534 90-99
GAGCT 7694880 2.9168272 30.935043 60-69
TGTGC 7035840 2.850097 58.18165 25-29
ATCTG 5707975 2.7997048 37.304264 200-209
GTGAA 6335790 2.7987437 39.55485 10-14
GGAAG 8176855 2.7914336 31.337328 80-89
CAGGG 9466955 2.7733064 37.148205 80-89
GACAA 6449025 2.7698405 25.051012 180-189
GTGTA 5788675 2.7326016 37.43974 230-239
ACCTT 5355525 2.7293878 73.22543 40-44
GAACA 6345970 2.7255785 35.548885 210-219
ATCTC 5346300 2.7246866 48.34882 170-179
CTATG 5537570 2.7161229 28.635794 50-59
ATGAA 4970720 2.6586933 27.962816 210-219
GGGAA 7734950 2.6405752 34.891853 80-89
GAGTC 6917170 2.622028 29.272955 150-159
AGAAC 6056045 2.6010563 30.903366 190-199
CACGG 8518500 2.5928876 44.53013 230-239
TGCAA 5612465 2.576019 36.45087 200-209
ATCAG 5589330 2.5654004 28.434578 45-49
CCCTC 7258075 2.5488417 27.03596 20-24
AGGGC 8679570 2.542645 33.296444 150-159
CGCCA 8002760 2.5310075 39.83528 70-79
CAGTG 6651040 2.5211484 76.3417 8
CCAAG 6808030 2.509161 40.057716 180-189
TATAT 3381630 2.5011 25.171202 240-247
CTACA 5242915 2.5003526 19.970823 190-199
AGGCT 6550665 2.4831002 20.96162 25-29
ACGGC 8121985 2.4721951 49.342754 230-239
GTGGA 6718880 2.451166 27.332863 100-109
TCTGT 4624740 2.4241097 25.648304 210-219
CACTG 6122530 2.4114196 20.16285 30-34
GCAGC 7921555 2.4111876 34.72945 30-34
CTGTC 5703160 2.4004478 50.01887 30-34
CTACT 4679350 2.3847823 23.57328 50-59
TGAAC 5123110 2.351414 38.362778 210-219
CTCTC 5338775 2.3348088 97.60221 20-24
CCGCC 8586635 2.3303604 33.9461 70-79
CAAAT 4192665 2.3300867 36.149834 200-209
ACAGC 6309935 2.3255837 27.393808 210-219
TATGC 4735800 2.3228626 21.912922 50-59
GGGCT 7413875 2.3209596 42.68522 90-99
TCCAA 4859455 2.31748 44.29933 180-189
CTTCA 4516465 2.3017695 50.80045 45-49
GTATC 4663090 2.2871993 23.112055 200-209
GGCCG 9082280 2.2831206 25.738594 230-239
GTGGT 5842145 2.2776287 32.14364 120-129
TGGTG 5809845 2.265036 35.477016 35-39
CAGGC 7395825 2.2511644 33.649883 70-79
ACAAC 5026880 2.243325 20.276386 180-189
CAGTA 4884720 2.241997 22.216534 45-49
AAATG 4182630 2.237167 34.40213 200-209
GCCTC 6610565 2.2342255 72.761894 4
AATGA 4162435 2.226365 26.44877 210-219
GCTCC 6546450 2.2125561 33.937428 80-89
TACAT 3694285 2.1940532 18.945871 190-199
GTAGT 4641135 2.190894 26.300669 120-129
GCCTG 6702730 2.1802542 31.051619 210-219
TATCA 3645540 2.1651032 17.705664 170-179
GGGGG 9294295 2.1641352 96.52189 5
GCTAT 4407345 2.161759 34.981335 50-59
GTGGG 7053135 2.1250613 30.203226 100-109
CCTTC 4857825 2.1244748 55.00522 45-49
TCTAT 3312935 2.1026375 21.020433 110-119
GCAGA 5890760 2.0895147 24.695396 140-149
CCTCT 4756555 2.0801864 49.056026 35-39
TGCAG 5486835 2.0798442 44.04233 30-34
GCTGT 5129585 2.0779057 19.430532 230-239
AACTA 3735980 2.0762827 38.40839 130-139
AACAG 4744625 2.0378046 34.219875 210-219
TGCAC 5151060 2.0287967 27.715536 25-29
AGCAC 5498155 2.026395 21.447783 130-139
CAGAG 5690865 2.0186098 32.900795 170-179
TGAGC 5324485 2.0183036 18.573612 200-209
TGGTA 4268205 2.0148487 32.100155 120-129
ATATC 3389605 2.013102 14.633455 160-169
TGTGA 4264010 2.0128684 19.914604 210-219
CTCAC 4910330 2.0094888 55.456985 20-24
ATGGA 4521430 1.9972764 12.569011 100-109
AGAGT 4502400 1.9888701 31.860981 150-159
GGCTC 6094135 1.982291 20.875433 40-44
CATAT 3324520 1.974448 15.433917 160-169
CCGTG 5974945 1.9435211 20.638216 230-239
TCCGC 5740640 1.9402097 42.06982 70-79
GGTCT 4784965 1.9383062 36.04865 15-19
TCTCT 3545905 1.9311903 33.335358 35-39
TTCAG 3935700 1.9304216 47.603508 45-49
CTGTA 3926095 1.9257104 24.785574 190-199
GCACA 5197985 1.9157647 19.15223 130-139
CAGAC 5195585 1.9148799 26.679895 140-149
CTATT 3001520 1.9049901 21.073965 110-119
GGATC 5014670 1.900865 18.439802 70-79
ACTAT 3188330 1.8935641 17.568756 130-139
TCCAT 3714925 1.8932731 27.583715 45-49
TGGGG 6268265 1.8885853 125.637085 4
GTCAC 4789375 1.8863435 38.82496 160-169
ACCAG 5098395 1.8790597 14.228313 190-199
CCAGA 5083240 1.8734741 34.424393 170-179
GCCAG 6153460 1.8730094 35.58518 70-79
GGTGG 6216280 1.8729224 18.759193 40-44
AGAGA 4500985 1.8605235 28.744287 170-179
CAGTT 3785055 1.8565315 25.599482 190-199
AGGAC 5230820 1.855427 21.040678 220-229
GATGG 5079220 1.852989 21.125574 100-109
TGGGA 5070015 1.8496308 17.037737 100-109
GCTGA 4875135 1.8479725 27.98016 200-209
ACCCT 4514015 1.8473022 43.984028 10-14
CCTGC 5457735 1.8445941 50.15017 20-24
TGTCT 3485870 1.8271581 51.506683 30-34
ATACT 3070240 1.82343 27.64915 130-139
TTCCA 3575080 1.8220024 23.226746 180-189
TAGTG 3848020 1.8164961 27.05149 120-129
TCAGC 4598665 1.8112304 11.58933 45-49
CACAG 4914300 1.8112098 16.154467 140-149
TAGTA 3167675 1.8106074 17.668976 120-129
GAGGA 5296080 1.807988 27.500814 220-229
GCCGA 5918635 1.8015326 19.784758 220-229
AGTCA 3924700 1.8013657 30.026178 160-169
TACAA 3217525 1.7881498 18.854506 130-139
CAAGG 5031295 1.7846533 17.54482 80-89
AGTTC 3598015 1.7647905 22.847076 190-199
GCAAA 4093755 1.7582577 33.053623 200-209
CATGA 3810395 1.7489017 24.011574 60-69
AGCTA 3805305 1.7465656 26.70371 50-59
ACCTG 4412135 1.7377634 30.815079 25-29
TGACC 4389255 1.7287521 14.887638 210-219
TTCTG 3291180 1.7251092 43.444775 30-34
CTGAA 3753580 1.7228248 24.177395 200-209
GGAGC 5833600 1.7089297 13.315145 200-209
GTGCA 4496710 1.7045265 51.176422 25-29
CTCAG 4320690 1.7017472 79.5786 6
CCTGG 5181195 1.6853312 34.532482 2
ATTGG 3557570 1.679386 21.620426 100-109
GGATG 4598745 1.6777033 22.544535 100-109
ATGCA 3653705 1.676984 16.379442 60-69
AACAC 3744240 1.6709266 22.91594 190-199
CAGCT 4139495 1.6303817 13.4132805 50-59
AGGGG 5747725 1.6205052 33.360703 90-99
GCCGT 4951810 1.6107174 30.04824 230-239
TCTGC 3825610 1.610191 33.71474 200-209
CGATT 3251120 1.594642 35.385063 160-169
TCTCA 3117355 1.5887277 26.974955 100-109
GGGGC 6561890 1.5875591 28.803072 90-99
CTCCC 4517090 1.5862811 14.248974 190-199
GGGCC 6293635 1.5821059 28.963575 2
ACAAT 2810425 1.5619025 23.734396 180-189
TCTGA 3184125 1.5617815 21.596815 220-229
AGTTA 2731780 1.5614548 16.913443 50-59
GTTCT 2968735 1.556096 24.055195 190-199
GCACT 3949175 1.5554222 26.669771 25-29
CGTCC 4536945 1.5333875 19.361538 180-189
GGCTT 3781310 1.5317429 22.148243 25-29
CCATG 3882765 1.5292658 15.91874 160-169
TTCTC 2807850 1.5292269 22.91165 190-199
TTAGT 2501655 1.5280792 26.528162 110-119
AGTGA 3457590 1.5273404 88.70614 9
GAGCA 4301175 1.5256722 7.871053 200-209
CGGCC 5794630 1.5135362 31.845789 230-239
GTTAC 3066535 1.5041049 16.659948 50-59
CTTTA 2368630 1.5033107 43.664886 45-49
CATCA 3123885 1.4897847 32.75969 45-49
GTCCG 4576465 1.4886258 34.326164 70-79
ACTCC 3519060 1.4401298 17.262856 140-149
TAGCA 3137335 1.4399796 12.345889 130-139
CTGGT 3543470 1.4353981 32.525654 35-39
TACGC 3621420 1.4263326 25.087852 140-149
CTCAA 2986270 1.424156 19.072271 150-159
GTGAC 3753390 1.4227631 15.865084 210-219
GTCCA 3571850 1.406809 29.36204 180-189
GGGGT 4667785 1.4063717 120.296585 7
AAGCT 3058330 1.4037178 17.611639 200-209
GGCAG 4786850 1.4022884 13.781963 150-159
CAACT 2935055 1.3997313 18.46577 130-139
CAACC 3653190 1.398983 15.649079 140-149
AACCC 3590320 1.3749071 20.941265 140-149
AGCAG 3866100 1.3713465 13.0238 50-59
ATCTA 2298960 1.3653631 21.612549 110-119
GAGAG 3989515 1.3619498 26.270542 220-229
CGTGT 3361665 1.361752 24.66999 230-239
CTACG 3456155 1.3612413 16.71773 130-139
GAACT 2949165 1.353613 14.839862 190-199
CCATA 2832270 1.350713 15.730647 160-169
CGACA 3647050 1.3441534 16.55989 70-79
CACGT 3412290 1.3439647 16.829956 180-189
TTACA 2258070 1.3410784 10.182694 120-129
CAATT 2248105 1.33516 24.80734 180-189
TCAAG 2898840 1.3305147 24.156971 150-159
GGCCT 4089665 1.3302801 69.872734 3
CCTTT 2426525 1.3215474 49.57639 45-49
ACAGT 2877220 1.3205914 12.766115 120-129
TGGAA 2983195 1.3177834 19.49654 120-129
GTGCG 4207240 1.3171027 17.534605 240-247
AAGAG 3173940 1.3119773 19.837393 150-159
ATACA 2353865 1.308168 22.44907 35-39
TGTCC 3096820 1.3034449 54.77147 15-19
GTGGC 4162065 1.3029604 14.764729 40-44
AATTC 2189865 1.3005711 24.794804 180-189
TGCGA 3426740 1.2989427 14.82274 240-247
ACATG 2822195 1.2953359 14.904872 190-199
AACCA 2902610 1.2953359 17.817728 190-199
ACAGA 3006420 1.2912499 20.490366 140-149
CCAGT 3276190 1.2903603 19.483322 190-199
ACATA 2318490 1.2885082 21.760122 130-139
TGTGT 2550405 1.2865927 15.113478 240-247
TTATT 1625005 1.2843827 20.245813 240-247
TCACT 2503335 1.2757988 13.636498 190-199
AGAGC 3595440 1.2753406 25.150326 220-229
TCCGT 3027645 1.2743292 13.800352 150-159
ATATT 1719850 1.2720248 26.824928 240-247
GATTG 2686950 1.2684015 24.831161 100-109
ACGTC 3174480 1.2503006 18.677402 180-189
AGACC 3384475 1.2473791 102.53173 9
ATCCG 3160480 1.2447867 24.54838 70-79
AGTCG 3255875 1.2341746 16.82792 150-159
ACACA 2763400 1.233211 7.7374206 130-139
CCTAC 3011525 1.2324276 17.318178 190-199
ATTAT 1660090 1.2278254 9.460062 50-59
CTTCT 2246890 1.2237136 23.18951 30-34
CGGCT 3738110 1.2159269 21.20238 230-239
TGGAC 3194660 1.2109704 16.170012 80-89
ATTAG 2101950 1.201451 26.178757 110-119
TACCA 2516150 1.1999551 11.141909 45-49
GTCGA 3152870 1.1951294 13.134937 150-159
TGAGT 2529685 1.1941628 21.360083 90-99
GATCC 3013345 1.1868362 24.374973 70-79
TCGGA 3098915 1.1746771 97.155266 5
ATCAT 1974365 1.1725847 15.84847 110-119
ACGCA 3179535 1.1718464 21.547922 140-149
CTTCG 2782985 1.1713523 108.67886 3
GAAGT 2625545 1.1597966 13.793758 140-149
AGCTC 2943595 1.1593645 12.698714 210-219
ATTCC 2271865 1.1578325 22.720673 180-189
GGACT 3049170 1.1558207 13.527165 90-99
GGGTG 3818550 1.150503 14.7229 60-69
TCGAG 3029620 1.1484102 12.784931 150-159
GTATA 2007565 1.1475016 10.540047 240-247
AAGGC 3232550 1.1466196 29.354364 25-29
TTGGG 2928130 1.1415658 16.050152 100-109
CGCTG 3494065 1.1365443 12.521064 190-199
GATCT 2311225 1.1336329 9.488481 220-229
AGGCC 3685550 1.1218193 14.281484 2
GAACC 3040150 1.1204749 11.1192 180-189
TTCGG 2758750 1.1175216 104.679 4
ACAAG 2595530 1.1147735 17.91508 80-89
GCAAG 3140190 1.1138586 16.891098 25-29
GCCCC 4090480 1.1101315 12.373975 80-89
GAAGC 3121285 1.1071527 13.707837 200-209
GACCC 3498195 1.106363 42.960365 10-14
CCCCT 3148135 1.1055408 10.042795 140-149
CACAT 2314300 1.1036925 14.468324 130-139
TAGAC 2395100 1.0993072 18.267113 170-179
CGCAG 3606820 1.0978552 16.849957 140-149
GCTTC 2605770 1.096763 28.76727 2
CTTGA 2235950 1.0967112 22.499977 90-99
AGGTC 2889530 1.0953076 18.275084 10-14
TTGAG 2309305 1.0901304 21.065565 90-99
CCGAT 2763355 1.0883751 23.589394 160-169
GCACC 3421090 1.0819774 11.301296 130-139
ATCAA 1927120 1.0710031 12.814799 110-119
ATGGG 2933040 1.0700247 20.073936 100-109
CTATA 1794195 1.0655808 13.7122555 50-59
ATGAG 2398440 1.0594763 20.677422 60-69
GGAGA 3101595 1.0588297 87.22653 7
TAGTT 1720565 1.0509682 15.79099 50-59
TGGCT 2592950 1.0503591 32.563183 40-44
TACAG 2286905 1.0496476 9.361572 120-129
AGATC 2282205 1.0474905 12.960997 210-219
GCTCT 2488260 1.0473033 17.943937 210-219
GAGCC 3437480 1.0463109 18.35618 220-229
ACACC 2729380 1.0452114 13.055971 40-44
ATGGT 2201830 1.0393957 9.89875 35-39
CTCCG 3075245 1.039365 9.8874855 140-149
CAGCA 2808690 1.0351682 12.630278 45-49
GTAGC 2720595 1.031271 11.382501 50-59
GGGAG 3653815 1.0301512 13.662932 5
CGTGA 2699980 1.0234566 20.807198 150-159
ACCGC 3223200 1.0193912 11.281427 220-229
GACAG 2873265 1.0191774 15.98891 70-79
AAACT 1819295 1.0110791 17.65094 130-139
CGAGG 3445955 1.0094787 14.95283 220-229
AGCCG 3310290 1.0075965 19.395758 220-229
GTAGA 2275010 1.0049528 17.980371 170-179
TTTAG 1638570 1.0008835 31.534243 45-49
ACCAA 2238025 0.99875414 17.937546 130-139
TGAGG 2727215 0.9949361 11.923386 220-229
CGAGT 2616410 0.99177855 15.297473 160-169
ATGAC 2155030 0.98911947 11.319579 160-169
TTTCA 1558120 0.9889001 7.414256 100-109
GCCAA 2680650 0.9879779 15.042764 180-189
AAGGT 2224660 0.98271143 20.75368 15-19
ACTCA 2051080 0.97816265 12.59867 190-199
GGTAT 2072080 0.97814584 9.4757595 110-119
AACTC 2048815 0.97708243 16.460075 190-199
TTATA 1318175 0.97494036 13.246552 110-119
CCAAC 2545005 0.9746054 14.7027445 130-139
CCGAG 3158030 0.9612511 18.304234 220-229
CATAC 2004710 0.9560487 14.738833 130-139
GGGAC 3255170 0.95358896 10.384031 90-99
CCGTC 2789825 0.94289935 10.233329 140-149
CGGAC 3088730 0.94015735 10.981195 220-229
ACAGG 2640790 0.93671596 15.545324 70-79
AGCCC 2961445 0.9366069 11.687972 80-89
CAGAA 2180210 0.9363947 18.509151 140-149
GATAC 2036875 0.9348885 23.967121 35-39
GCCAT 2373480 0.9348189 7.87765 60-69
AATCA 1678525 0.93284565 14.365544 110-119
AACTG 2021730 0.92793715 11.966418 60-69
TATGG 1963730 0.92699814 12.723982 35-39
ATGAT 1619570 0.925728 8.789522 120-129
TAACA 1658625 0.9217862 8.820823 120-129
TACAC 1929720 0.9202859 22.59264 35-39
GCAGT 2427070 0.92000717 6.1705422 50-59
TGGTT 1808030 0.9120897 9.493335 50-59
CCCAG 2881145 0.91121054 15.1989355 80-89
TCATA 1533835 0.9109518 8.985481 110-119
AAGTT 1587495 0.9073943 17.863276 140-149
CCCCA 2748630 0.9032395 17.243916 80-89
GAGAT 2043675 0.9027639 12.469187 210-219
TTTAT 1138515 0.8998675 10.749875 240-247
GACTG 2370960 0.898738 13.205471 90-99
CGGAG 3012920 0.88262284 75.00725 6
GTTAT 1439075 0.8790264 10.716843 110-119
GGTAG 2405515 0.87757415 12.481411 120-129
AGAAG 2114790 0.87416786 16.282816 140-149
TCCTT 1604025 0.8735929 10.813343 1
CGTAT 1780865 0.87349653 7.742141 240-247
TATTT 1086475 0.8587356 10.498036 240-247
ATAGT 1494835 0.8544308 15.853836 120-129
ATCCA 1782365 0.8500121 10.075376 180-189
TTACC 1666445 0.8492865 9.903435 45-49
GATCA 1835915 0.8426515 9.28001 110-119
CATGG 2218440 0.8409236 8.626298 200-209
GCCTA 2129915 0.8388884 16.65894 190-199
GTTTC 1596900 0.83703315 5.131805 100-109
GATGA 1890095 0.8349222 6.711586 60-69
ACGCT 2114985 0.8330081 15.417568 190-199
TCTTC 1528300 0.8323512 47.745026 2
GGTTC 2053480 0.8318291 11.765119 160-169
GTCTA 1684965 0.8264585 10.35634 30-34
AGTCT 1684005 0.8259877 15.195647 8
CTATC 1605435 0.8181934 8.183254 110-119
GCTTG 2016545 0.81686735 17.226072 90-99
CCTCC 2307210 0.8102304 10.999314 140-149
ACAAA 1557010 0.80972695 13.295993 130-139
ACATC 1696450 0.80903906 5.9224854 110-119
GGTGC 2582165 0.80836266 11.828181 60-69
GTCTG 1994940 0.80811554 14.089743 1
TCATC 1581840 0.80616844 10.432073 110-119
ATATG 1405555 0.8033994 5.973552 50-59
TGGCA 2111445 0.8003661 5.58861 100-109
ATATA 1149655 0.7956788 10.996317 130-139
TATAC 1322000 0.78514194 9.16306 130-139
CATTA 1308400 0.77706486 12.511112 110-119
TTATG 1267645 0.7743122 11.29235 50-59
GTTCA 1575455 0.77274495 14.307514 160-169
TGATG 1602855 0.75664365 11.799338 120-129
GGTTA 1592780 0.75188756 8.42712 50-59
CCAGC 2371080 0.7498939 6.2063503 80-89
CCCCC 2658590 0.7496948 13.740385 80-89
CTGAC 1881255 0.7409512 9.669648 220-229
GGGAT 2030260 0.7406746 13.507663 100-109
GACCG 2422995 0.7375188 9.709594 210-219
GCGGA 2517480 0.7374857 9.348259 220-229
TCAAC 1539790 0.7343278 7.7128186 110-119
CATCC 1787345 0.73144776 6.6571617 110-119
TGACT 1481480 0.7266512 5.0918345 2
ATCTT 1135985 0.7209814 13.389755 3
AACGC 1956090 0.7209347 14.654616 180-189
TTTAC 1132460 0.7187443 13.534489 45-49
GATTA 1255935 0.71787834 5.610869 160-169
TCCGG 2199945 0.71559477 12.050369 70-79
TTGGA 1514460 0.71491593 6.455284 60-69
ACGCC 2257480 0.713966 13.0499525 180-189
ATGCC 1812625 0.71392053 8.459748 50-59
GGGCA 2429205 0.7116258 13.774005 150-159
TGCCA 1801105 0.7093833 7.568233 60-69
ATAAA 1090130 0.7060147 7.731305 130-139
TATTG 1155140 0.7055911 6.401384 240-247
GACCA 1910175 0.7040124 6.1637273 170-179
CAACG 1904425 0.7018931 14.904808 180-189
GAAAT 1304120 0.6975358 7.4507527 110-119
CGCCG 2665470 0.69621104 11.044675 220-229
GGATA 1568695 0.69294834 15.428952 35-39
CACAA 1551205 0.69224983 8.4169035 130-139
TATAG 1209960 0.6915995 6.42082 120-129
CAAAC 1545220 0.68957895 14.2005825 130-139
AATAC 1238620 0.688367 11.42378 130-139
TTTCT 1000445 0.6785463 9.585987 4
GCCGC 2536610 0.66255325 12.131348 220-229
AAATA 1010100 0.65418386 12.685715 130-139
GCGAC 2136140 0.650205 7.203945 70-79
AGGCA 1825815 0.6476359 6.436752 80-89
CAGTC 1644265 0.6476102 5.318708 210-219
CACGC 2047465 0.6475452 12.4197235 190-199
CCCGT 1902655 0.64305544 10.037702 140-149
GACGA 1795305 0.63681364 9.943519 220-229
TCCAC 1546635 0.63294035 10.454698 180-189
TCAAT 1057505 0.6280572 8.659982 110-119
ACTTC 1231030 0.62738174 29.832396 4
TACTT 987710 0.626875 12.242735 1
CCGGC 2388025 0.6237433 9.729907 70-79
AGATG 1411890 0.623682 5.826535 120-129
AAGCA 1426145 0.61252576 6.003732 120-129
AATAA 945455 0.612317 7.205264 130-139
TAGCT 1244800 0.610562 12.621354 50-59
CACGA 1652195 0.6089314 9.05749 180-189
CCCTA 1478780 0.60517156 11.962474 110-119
CACCG 1907200 0.6031841 9.133112 45-49
AATGG 1345535 0.59437066 6.782468 120-129
GGCCC 2267820 0.59234625 10.369055 70-79
TTAGC 1198890 0.5880436 10.2309885 45-49
ACGAC 1580865 0.58264214 9.261896 220-229
AGTAA 1083455 0.5795085 5.073843 50-59
CCGTA 1455705 0.57334393 6.6943173 230-239
CTTTG 1092980 0.5728978 6.91773 45-49
CGGTT 1407115 0.5699979 11.665821 160-169
ACCCG 1797635 0.5685323 8.7782955 140-149
GTTCC 1347705 0.56724614 6.7829585 140-149
ACCTA 1187565 0.5663512 7.143256 130-139
GGGGA 1993615 0.5620769 8.890291 4
TGATT 919510 0.5616619 5.9673557 50-59
CGGCA 1840865 0.56032825 11.21079 70-79
ATTTC 877055 0.556645 6.2411747 240-247
TAATA 799175 0.5531108 7.9169927 130-139
GGTGA 1508550 0.55034554 6.3135347 120-129
CTGCG 1682540 0.54729414 11.968114 25-29
TCAGG 1443660 0.5472349 5.41259 150-159
TTCAA 914280 0.54299515 5.3695164 200-209
ATTAA 782940 0.5418745 6.3396883 110-119
CCACG 1704265 0.5390025 7.904593 180-189
AAATC 954170 0.53028303 8.043832 110-119
ACCCC 1611375 0.5295211 9.497007 140-149
GCTAC 1339045 0.5273963 7.969089 50-59
CCTAT 1030900 0.5253875 5.2052426 110-119
GAAGA 1256280 0.51929486 6.164458 15-19
CTGTT 986860 0.51727384 5.750088 240-247
GTCCT 1228120 0.51691306 6.4379735 45-49
TATGT 845265 0.51631093 6.750159 140-149
AAGAT 961310 0.5141767 5.6715794 15-19
ACATT 855370 0.5080082 6.282106 110-119
GGGTA 1386570 0.5058452 5.546128 100-109
TGACG 1285010 0.48709694 8.787704 220-229
GTAAC 1056480 0.48490506 7.040013 120-129
AGTTT 792375 0.48400435 5.0559244 140-149
CGCGG 1919405 0.4825037 6.549776 220-229
GGGTT 1225045 0.47759816 5.789594 100-109
AGTAT 828155 0.47336408 5.1093574 110-119
TATCC 924865 0.4713479 5.3970127 110-119
CCGGT 1441915 0.46902388 9.07819 160-169
TGGCC 1439165 0.46812946 14.83964 2
CCATT 904270 0.46085182 5.068731 110-119
AACAT 828090 0.46021366 5.9764237 110-119
CAATC 959065 0.45737925 6.463136 110-119
CATAA 822785 0.4572654 6.1674957 110-119
GGTTT 900905 0.45447594 5.484098 100-109
ATGCT 912605 0.44762367 7.019274 50-59
CCGCG 1700475 0.44415778 6.757979 220-229
TAAAT 637100 0.44093826 7.745021 130-139
TGCTA 893830 0.4384147 6.271453 50-59
TGCGC 1311815 0.4267052 8.961782 25-29
ATTCT 671540 0.42620975 7.120901 2
TTGGT 844200 0.4258702 5.2732 120-129
GGTCA 1112490 0.4217013 5.2884765 160-169
GCATG 1099280 0.416694 8.40049 60-69
GCCTT 982170 0.41339323 8.691296 1
GCGCT 1267830 0.41239786 8.148974 25-29
ACGAG 1147140 0.4069026 7.9419103 180-189
CTAAC 840560 0.40086412 5.662349 110-119
ATGGC 1045480 0.3963005 7.535018 1
CACTT 771365 0.39311817 7.000901 3
CCTAA 821120 0.39159316 7.7018056 110-119
CAATG 850900 0.39054754 5.0874 120-129
TACCT 761435 0.3880575 5.033808 2
AGCTT 790765 0.38786235 7.558965 1
CCGGG 1524925 0.38333854 5.169814 80-89
GGTAA 864375 0.38182515 7.3826046 120-129
CATGC 962015 0.37889928 6.8196206 60-69
TTTGA 614585 0.37540534 5.8195963 45-49
GAGGC 1267570 0.37132952 5.904213 35-39
TTCTT 533310 0.36171457 20.520115 1
TGGGC 1094245 0.3425602 9.46602 1
CGAGC 1121195 0.34127286 6.678678 180-189
TGCTG 835070 0.33827233 14.107266 1
ATGTG 699340 0.3301304 5.0977373 140-149
AACCT 657155 0.31339806 5.158694 2
CTCTT 567595 0.3091267 9.2677145 1
GCGAG 1025790 0.30050108 5.4688606 240-247
CGCTT 713735 0.30040953 5.901195 1
AACTT 489415 0.29066586 10.762519 3
GACTT 581040 0.28499433 6.887219 1
TTCCT 515760 0.28089604 6.3131046 4
CTCGG 857970 0.2790792 5.294984 6
TCACA 572440 0.27299735 5.062332 5
ATTGT 443820 0.2710974 5.2392025 240-247
GAGGT 720190 0.26273802 6.1522045 7
CCCTT 589800 0.2579375 5.456095 3
TAACT 431485 0.25626096 6.777899 2
GTCTT 480095 0.2516472 12.04684 1
TGTAA 435575 0.24896978 7.5685554 25-29
AATCT 415375 0.24669318 7.680152 2
TTGGC 607075 0.24591556 7.0669026 1
CGGGG 999355 0.24178024 8.737183 3
CCTCG 671420 0.22692516 5.773059 5
TGCTT 403410 0.2114519 11.665881 1
TCGGT 483300 0.19577643 6.354895 7
TGCCT 445195 0.18738161 5.28023 4
TCGCA 396460 0.15614974 5.3445153 7
TAGGC 402465 0.15255871 7.0200953 1
TTGCT 234610 0.12297348 5.467978 4
Produced by FastQC (version 0.10.1)