FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_5_SL248351.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_5_SL248351.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11034049
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 12857240 8.850772 63.15936 110-119
ATACA 11759925 7.6750474 39.12969 110-119
CACAG 17188915 7.6100636 43.260956 110-119
TCCCT 20171820 7.133613 109.676125 30-34
CAGTA 14582750 6.8098273 40.56555 60-69
AGTAA 11142535 6.4968615 49.122757 110-119
GGAGA 17744850 6.270445 96.184685 35-39
CCCTG 20230425 6.059765 71.885 30-34
GTAAT 12150940 5.9849896 40.66004 110-119
GGTGA 19821255 5.916857 103.12531 45-49
AATAC 8525035 5.563815 41.00632 110-119
ACAGT 11414910 5.330515 36.43118 110-119
GTAGT 15092890 5.3191967 27.712446 80-89
AGACG 13307170 5.2634273 103.23508 40-44
GTGAC 15171640 5.069316 85.71511 45-49
CCCCA 12060450 4.786711 102.7031 60-69
GAGAC 12065225 4.772197 142.92047 40-44
ACTCC 11364420 4.75749 123.73386 30-34
CTCAG 12615275 4.718135 35.595512 140-149
ACCAG 10485225 4.642133 97.088615 45-49
TAGTA 9219315 4.541007 35.389233 80-89
CACTC 10581105 4.42957 101.810875 25-29
GCACA 9977620 4.4174004 34.993633 110-119
GGCCC 17338040 4.3988276 59.774193 60-69
CGTGT 15377060 4.340342 23.715939 120-129
GTGTC 15357435 4.334803 24.17383 120-129
TGACC 11539030 4.3156176 146.42381 45-49
AGTAG 10192025 4.2520685 28.6607 80-89
CTCCC 12630980 4.2349143 71.21402 30-34
ATGCA 8812100 4.115059 99.17876 25-29
GACCA 9168920 4.0593643 109.62547 45-49
CCCAG 11332780 4.0184016 87.844734 60-69
CCACT 9126875 3.820786 28.52393 240-247
TGAGG 12791545 3.818413 70.178154 35-39
TACAC 7211045 3.7692225 28.52324 110-119
AGGAG 10642015 3.7605374 96.77289 35-39
ATGGT 10609780 3.7392116 25.996096 190-199
CAGGG 13138380 3.7183042 36.66617 50-59
ACGGT 11041305 3.6892426 97.583115 40-44
GACGG 12991950 3.6768627 109.288124 40-44
AGATA 6298895 3.6726875 20.154196 150-159
CTGAG 10963155 3.66313 106.50236 35-39
TGCAC 9608930 3.593757 119.25041 25-29
GAGGA 10055330 3.5532222 126.93712 35-39
TGTCC 11192355 3.5361366 20.76667 120-129
CCCTT 9982910 3.5303817 33.358658 20-24
GGGTT 13862850 3.4957976 43.30466 50-59
CCTGA 9249975 3.4595075 87.2972 30-34
GCCCC 12125775 3.443526 73.51495 60-69
TGGTG 13587620 3.4263928 18.085257 190-199
GGATG 11298670 3.372774 71.92575 20-24
CCAGG 10598905 3.3575385 41.572567 50-59
GCACT 8940820 3.343883 92.27877 25-29
TCTTG 9996865 3.3314111 18.177341 170-179
CCGTG 12314435 3.2954013 23.900396 120-129
CTCTC 9302000 3.2895823 13.868128 100-109
AGGCT 9833975 3.2858362 23.514427 30-34
CAGAT 7014705 3.2757149 18.377495 150-159
CTTCA 7392850 3.2643697 17.232327 200-209
TCAGG 9709170 3.2441347 21.284653 140-149
CTTGG 11477555 3.2396643 18.918812 20-24
TCGCA 8580270 3.2090364 25.067316 100-109
CCTGG 11941350 3.1955621 30.134659 50-59
TGTCT 9450550 3.149354 19.977543 180-189
TCTCA 7108355 3.1387486 22.329073 130-139
CGCAC 8811210 3.1242979 25.59698 100-109
CGGAT 9224905 3.0823271 81.8085 20-24
GGTTG 12173725 3.0698507 74.79099 15-19
TTCCC 8673215 3.0672176 51.091866 50-59
ACAGA 5483920 3.0314744 14.529175 140-149
ACCAC 6103345 3.0245767 20.482325 230-239
GATGC 8906750 2.9760215 98.33925 25-29
GGTTC 10491675 2.9613893 48.924347 50-59
AGGGT 9855410 2.9419453 51.50115 50-59
TGGCC 10922830 2.9230013 43.907314 60-69
GACTC 7678340 2.871713 20.021572 190-199
GTTGG 11354175 2.8631847 54.043167 15-19
TCAAA 4368200 2.8508806 59.373974 70-79
GCCCT 9463750 2.834745 18.063873 20-24
TCTCT 7572320 2.8245566 15.100361 180-189
CGGTG 11514285 2.7527952 68.537506 40-44
CGGCC 10848355 2.7523324 13.145929 120-129
TTCTT 6985975 2.7485597 20.615328 170-179
CCAGT 7336575 2.7438924 37.343 60-69
AGTCA 5866430 2.7394953 32.87218 70-79
GCCGT 10215935 2.7338328 22.581205 120-129
GATGG 9131460 2.7258384 18.732916 15-19
TCACA 5176065 2.705536 11.655476 140-149
GTTCC 8556870 2.7034757 55.390285 50-59
AGATG 6358840 2.6528804 17.461008 180-189
TCCAT 5807490 2.5643413 17.172049 70-79
GCGGA 9058620 2.5636878 89.95537 20-24
AGGGA 7231840 2.5554938 9.328978 150-159
TGGAG 8345660 2.4912686 15.991783 25-29
TGTTC 7469980 2.489338 13.204413 140-149
TACCA 4756260 2.4861033 17.034065 230-239
TCCTC 7019565 2.4824164 17.040205 130-139
ACAGC 5592110 2.4757998 11.358054 120-129
GGCTG 10317790 2.466741 12.134408 30-34
GGCGG 12103000 2.450847 43.208588 20-24
ACTCT 5525485 2.4398203 13.544355 190-199
GAGAT 5771345 2.4077802 17.123009 180-189
TTGGG 9483915 2.3915608 81.387 15-19
CAGCT 6362670 2.37965 12.49622 150-159
GTTCT 7129175 2.375766 18.106068 160-169
CTGGC 8823920 2.3613226 35.551975 60-69
CACGG 7438525 2.356388 9.815397 120-129
CTGGA 7019430 2.3454092 14.90221 180-189
TGTAG 6565745 2.3139696 8.224029 210-219
ACTAC 4420340 2.3105173 13.41859 230-239
AATAA 2833865 2.309291 9.82459 110-119
CTACT 5163485 2.2799761 7.980453 230-239
CATTT 4886510 2.275847 17.910597 150-159
TCATT 4867425 2.2669582 16.644901 150-159
CACTA 4300860 2.248065 15.603019 240-247
GTGAA 5373335 2.2417321 18.294554 190-199
TTCAT 4802090 2.2365294 16.445272 140-149
CAGAG 5618325 2.222234 9.533408 140-149
GATAC 4720765 2.2044945 15.09136 150-159
TCAGC 5881160 2.1995642 14.946375 140-149
TTCAC 4978975 2.1985047 18.80679 200-209
TTGTC 6438590 2.1456316 13.504653 170-179
ATTTG 5134770 2.136527 15.739008 150-159
TGGGG 9953910 2.1260512 53.778835 15-19
ACACA 3430785 2.1228201 14.244014 110-119
TAGTC 5362595 2.1154635 27.183424 70-79
CTCGC 7020030 2.1027598 16.794653 100-109
ATATA 3049335 2.099126 11.002095 110-119
TTGGA 5914775 2.084548 15.975963 170-179
TCTGC 6575135 2.077362 12.081952 210-219
ATGTA 4204925 2.071151 7.5511317 70-79
TACAG 4427980 2.0677705 14.1599865 160-169
CTCTG 6540845 2.0665283 14.7416525 180-189
AGCTC 5512965 2.0618587 12.811746 140-149
GCTGT 7247090 2.04557 10.31666 140-149
GAATC 4375710 2.0433614 14.522237 190-199
GTCTC 6448340 2.037302 12.0026045 180-189
GAACT 4358655 2.035397 11.01201 160-169
GTCAA 4358625 2.035383 41.5945 70-79
TCTCG 6415205 2.0268335 16.168512 100-109
GTCCT 6411560 2.025682 14.303933 130-139
CAGGC 6387670 2.0234964 15.667813 140-149
AATAT 2912295 2.0047894 13.616605 110-119
GTTCA 5072990 2.0012188 15.337495 140-149
AACCA 3232400 2.0000682 15.81866 70-79
ATCTC 4521730 1.9966046 7.852016 100-109
TGAAT 4052535 1.9960908 13.969495 190-199
AATCG 4260710 1.9896591 13.507373 190-199
GATAT 4031995 1.9859737 8.569785 180-189
ACGGC 6259480 1.9828882 14.799179 120-129
TACTA 3595760 1.9824449 10.689828 230-239
TTGCA 4996565 1.9710702 14.494048 150-159
ACACG 4414985 1.9546503 13.688217 120-129
TGGAA 4677160 1.9512908 9.4988985 170-179
CCTTC 5497255 1.9440631 14.146444 200-209
ATATG 3931725 1.9365854 8.867994 180-189
TTTGC 5787000 1.9284922 12.221331 150-159
GAGTC 5770850 1.92822 12.4051895 210-219
CATAG 4111285 1.9198809 8.655798 210-219
CAGCC 5384580 1.9092759 10.353281 120-129
GGGGC 9368110 1.8970342 49.124805 15-19
TCGGC 7082805 1.8953922 8.1460495 130-139
AGTAT 3774570 1.8591782 12.280037 220-229
GGGCG 9118810 1.8465511 60.11905 20-24
GACGT 5406620 1.8065195 9.955047 170-179
AGAAC 3245815 1.7942648 11.640126 160-169
CTGTT 5365130 1.7879057 12.928286 140-149
GTTGT 5991490 1.7837843 7.023953 210-219
CGTAG 5300240 1.7709745 9.988829 210-219
GCAGA 4436355 1.7547257 14.957759 150-159
CCAGA 3962340 1.7542503 26.519695 60-69
ATTGT 4196935 1.7463031 7.695435 170-179
CACCA 3489760 1.7293874 13.760491 230-239
AGTCT 4383090 1.7290634 13.202692 210-219
CTCCA 4093175 1.7135267 8.033348 150-159
ATAGT 3459030 1.7037578 7.6550655 220-229
TGCAG 5090520 1.7009006 13.667386 150-159
GGTCA 5087390 1.6998549 7.8339024 130-139
TGACT 4285280 1.6904788 11.935073 190-199
GTCAC 4490225 1.6793522 8.816034 130-139
AACTG 3588985 1.6759781 10.922972 160-169
TCCCA 3992985 1.6715842 10.9043665 240-247
GAGAA 3364140 1.661422 10.64573 160-169
TGGTT 5533720 1.6474972 18.253706 10-14
TCAGA 3522390 1.6448795 10.450874 130-139
TAGTT 3933095 1.6365219 10.209948 220-229
TGTGC 5789120 1.634042 8.2967205 220-229
CAAAA 2114225 1.6334049 21.680922 70-79
ACTGA 3492365 1.6308585 9.078968 180-189
AATAG 2790890 1.6272801 9.789304 240-247
CCTTG 5117110 1.6167107 21.57994 20-24
GCTCC 5396765 1.6165316 6.5343494 150-159
GAACC 3645675 1.6140529 12.97338 70-79
TTGTA 3866950 1.6089996 9.207962 210-219
GTGCT 5693480 1.6070466 8.367301 220-229
GTCTG 5667140 1.5996118 9.128151 210-219
CAAAG 2888205 1.5965803 29.917252 70-79
CTGTG 5648540 1.5943617 5.4194365 210-219
TCTGG 5640775 1.59217 12.022006 180-189
TGCTC 5036370 1.5912013 5.3218384 150-159
ATAGA 2712260 1.5814335 10.102451 240-247
CTGGT 5585065 1.5764452 7.121302 160-169
TTCAG 3989340 1.573735 9.418501 150-159
GGCCG 6899295 1.5638127 13.376442 120-129
GCTCA 4180005 1.5633293 11.527366 140-149
AAAAA 1618695 1.5614593 8.155266 8
GTCCC 5190090 1.5546248 19.69269 50-59
GGACG 5488415 1.553281 7.3848844 170-179
TTGGT 5202100 1.5487673 22.928162 25-29
CCACC 3894910 1.5458636 10.016614 230-239
ACCAT 2947825 1.5408319 11.567747 45-49
CCCAC 3847705 1.5271282 10.645928 240-247
GCTCT 4703645 1.4860797 10.332137 130-139
CCTCG 4906540 1.4696913 9.714722 130-139
TATAC 2643855 1.4576325 7.149378 110-119
TGATA 2921405 1.4389486 8.05207 180-189
CACTG 3822860 1.4297564 7.3797693 230-239
CGACT 3822375 1.4295752 8.841668 190-199
CATGT 3613515 1.4254775 7.523423 70-79
AAATA 1742010 1.4195484 5.4010706 9
AATTG 2876690 1.4169241 9.592123 170-179
GCTGA 4204650 1.4049039 20.110441 30-34
TCGAC 3753435 1.4037914 9.52105 190-199
TGGAC 4198115 1.4027206 10.4527 170-179
CAGAC 3161125 1.3995278 15.590614 60-69
CTCTT 3712330 1.3847387 8.142577 200-209
GGGAG 5450015 1.377983 6.6830063 150-159
GAGGG 5442520 1.376088 8.449099 200-209
GCGTA 4110945 1.3735943 10.478209 210-219
CCATC 3260780 1.3650612 10.94786 240-247
CCTCA 3234565 1.3540868 7.364328 130-139
ACGGA 3420870 1.3530676 9.562612 200-209
CTGAT 3420370 1.3492848 7.5422254 180-189
ACTTC 3007450 1.3279626 6.5766335 220-229
GAATT 2688450 1.3242058 10.603517 170-179
CTCGA 3533340 1.3214755 8.544625 190-199
TCCAC 3149330 1.3184047 9.898336 240-247
TATGG 3739470 1.3179038 6.37571 180-189
CTGCT 4164750 1.31582 5.7885313 90-99
GTAGG 4387350 1.3096709 5.230848 160-169
AGATC 2800575 1.3078076 7.313531 130-139
CCATG 3471175 1.2982258 7.6545067 70-79
GTGGA 4343065 1.2964513 20.356155 25-29
CTTGA 3270710 1.290246 8.479436 200-209
ACGTG 3840925 1.2833722 9.546847 170-179
GTGTT 4308055 1.2825927 7.6317267 160-169
GATCT 3223320 1.2715515 5.7723055 130-139
AGCCG 4012855 1.2711987 9.186151 120-129
ACTAT 2301160 1.268695 6.3945746 230-239
GGCTC 4731735 1.2662346 8.709311 130-139
GGAGT 4233580 1.2637689 7.116411 210-219
ACCGT 3371290 1.2608687 18.304527 45-49
ATAAC 1920325 1.2532891 7.797467 80-89
AAGTA 2144045 1.2501252 16.230574 70-79
AAAGT 2143265 1.2496705 21.989578 70-79
GGAAT 2979045 1.2428446 8.840951 170-179
ACTCG 3320430 1.2418469 8.717115 190-199
ATCGG 3708110 1.2389945 8.068337 190-199
GGAGG 4893775 1.2373432 15.538911 30-34
GTCCA 3252310 1.2163697 16.048817 70-79
AGCTT 3082365 1.2159468 6.323023 150-159
ACTGG 3632960 1.2138846 7.2973285 160-169
GCATA 2597225 1.2128476 8.22182 210-219
GTGGT 4799805 1.2103678 10.355764 50-59
TTGAG 3397955 1.1975434 8.178308 200-209
TTGGC 4220905 1.1913964 7.887399 60-69
TTCCA 2693175 1.189192 7.7083006 230-239
TGGTC 4194530 1.1839517 11.723432 50-59
ATAAA 1446330 1.1786013 5.863806 7
TGCCC 3916715 1.1732017 6.6068616 60-69
ATACC 2239300 1.170485 7.4621634 70-79
TCACG 3094895 1.1574962 9.712357 200-209
ACTGT 2912535 1.1489514 5.6100526 230-239
AGAGC 2889365 1.1428397 7.2871695 140-149
TCTAC 2576550 1.1376954 7.4150248 180-189
TATCA 2056160 1.1336195 6.778985 70-79
ATCCA 2165100 1.1317005 16.063042 240-247
TCTGT 3378490 1.1258668 6.522909 210-219
CCATA 2145855 1.1216412 10.236045 70-79
AGGTT 3176000 1.1193197 23.923153 10-14
GTCTA 2826900 1.1151696 7.4792886 180-189
GCTTC 3519995 1.1121147 5.645404 150-159
AGTTC 2812485 1.1094832 5.4547343 160-169
CAATA 1691450 1.1039151 6.5698147 100-109
AAAAG 1598810 1.1035262 14.4669075 70-79
CTACC 2632850 1.1021906 8.998148 230-239
TACTG 2786580 1.099264 6.6902037 180-189
GAGCT 3281710 1.0965211 6.544923 140-149
TATGT 2634775 1.0963038 7.8158402 220-229
CGGCT 4067020 1.0883539 8.075201 130-139
CTCGG 4047905 1.0832385 8.448747 130-139
CTGCG 3955600 1.0585372 7.0772595 210-219
AGGGG 4184180 1.057929 5.1306434 60-69
CTGCC 3500910 1.0486526 5.835894 200-209
AACTT 1893300 1.0438302 6.0894613 200-209
GAAGA 2101825 1.0380121 17.394491 35-39
ATATC 1875645 1.0340965 7.31702 70-79
ATACG 2212675 1.0332712 5.5318804 120-129
CATGG 3090560 1.0326519 5.486948 190-199
GGGGT 4827730 1.0311527 8.028531 10-14
AGAGA 2068170 1.0213912 12.801371 35-39
TAGAT 2070885 1.0200219 7.8105836 240-247
GTCCG 3801395 1.0172713 5.0391107 130-139
CTGAA 2169590 1.0131514 14.210356 35-39
ATGGG 3390310 1.0120438 29.267836 15-19
TGCGT 3585305 1.0119913 8.379931 210-219
GCGGT 4232000 1.0117718 5.846552 130-139
TATAA 1448430 0.9970821 6.3982706 240-247
ACCCA 2002125 0.99217415 13.299074 240-247
CTTCT 2649740 0.9883814 5.5745544 210-219
TGCTA 2503520 0.9876011 5.0676813 220-229
CGAAC 2226040 0.98553675 13.599461 70-79
ACTAA 1504625 0.98198485 13.023541 240-247
CGGAG 3418790 0.9675547 6.699651 210-219
CAGCG 3042155 0.9636988 6.454391 160-169
AGCTG 2882840 0.9632462 5.1854854 90-99
TGTAA 1953605 0.96225524 7.0480146 230-239
TCCCC 2869130 0.96196175 5.092203 80-89
TAGGC 2812165 0.9396316 5.86196 160-169
ACAAT 1432950 0.93520653 7.5884776 100-109
CCCAA 1885940 0.9345975 10.102523 240-247
AGTTT 2243270 0.9334024 5.014288 220-229
CCATT 2108380 0.9309714 5.742681 50-59
GAGTT 2619655 0.9232467 5.247208 160-169
TGCAT 2335745 0.9214164 6.255904 210-219
CGTGG 3830630 0.9158136 7.1697745 50-59
TCGAA 1927190 0.8999558 17.041563 70-79
AAGAG 1809695 0.8937401 12.815645 35-39
CTATA 1613770 0.8897173 5.0263433 90-99
TGAAG 2114150 0.8820142 9.375621 35-39
CCAAT 1665870 0.87075245 10.822041 240-247
TTCTG 2609490 0.869601 5.4737053 210-219
CATCA 1647685 0.86124706 5.4968963 230-239
TGAAC 1842665 0.8604845 6.807428 190-199
CCAGC 2399290 0.8507454 5.874436 80-89
GGTGG 3967515 0.84741974 20.201488 25-29
CGGTC 3158930 0.84534466 5.656963 130-139
CTTCC 2366965 0.83705944 5.419694 230-239
ACGTC 2234835 0.8358323 10.958483 70-79
AGCGT 2498745 0.83490825 6.6603317 160-169
ATCAA 1278150 0.83417726 12.820124 70-79
GTATG 2366315 0.8339619 5.462274 220-229
AACCG 1851210 0.8195879 5.9888935 190-199
GAGGC 2889545 0.8177725 13.135473 30-34
TAACC 1534825 0.802255 6.2218666 80-89
GGGCC 3424445 0.7761939 15.353993 15-19
CTAAT 1404470 0.77432424 9.942148 240-247
AAAGG 1543975 0.7625111 9.244159 10-14
AAAGA 1102675 0.7610853 5.993911 8
CGTCC 2539940 0.7608064 8.977762 70-79
ATGAT 1528085 0.7526638 6.91334 240-247
TGAGA 1792870 0.74797755 5.7994766 240-247
AGCCA 1687190 0.74697113 6.1044817 80-89
AATCC 1413140 0.7386501 9.760944 240-247
GACCG 2304765 0.7301072 16.885605 45-49
GGTCC 2725915 0.7294678 11.953622 50-59
GTCGA 2167625 0.7242707 12.159452 70-79
TGGGC 3024795 0.7231574 16.6516 15-19
TGTTA 1709955 0.71149534 5.011921 230-239
GTAGC 2119930 0.7083344 5.5098314 80-89
AAGGT 1694720 0.70702976 10.207396 10-14
CATAC 1349640 0.7054586 8.80786 70-79
AAAGC 1231940 0.6810083 9.907025 70-79
CATTG 1694750 0.6685535 5.2430706 50-59
TATGA 1341600 0.66080993 5.7434254 240-247
CAATC 1254320 0.65563464 9.463837 240-247
TAGGT 1854660 0.653639 5.508834 10-14
AGGAT 1520780 0.63446283 5.5935383 10-14
GAGGT 2118940 0.6325262 7.0510063 10-14
GGAAG 1776300 0.62768584 5.692121 30-34
ATAGG 1493215 0.62296283 5.3064156 10-14
AGACC 1381835 0.61178106 5.1832438 240-247
AGAAG 1226515 0.6057295 5.3699 9
CCGTA 1607085 0.6010527 5.121592 80-89
AATGG 1411635 0.588928 6.6176376 10-14
CATCC 1403865 0.58770037 7.5738792 240-247
CGATG 1735290 0.5798142 12.865121 15-19
GGGTC 2377865 0.56849164 5.7459183 70-79
TAAAA 684670 0.5579314 5.909244 8
TGATC 1408010 0.5554389 5.555756 240-247
AAGAA 783060 0.54048145 5.3360553 9
GAAGG 1511975 0.5342821 5.617047 10-14
AAGGG 1484575 0.5245998 6.513271 10-14
GAAAA 747735 0.5160995 6.312118 8
AAGCA 931245 0.514786 6.527639 70-79
GGTCG 2052095 0.49060774 5.8439317 70-79
GATCC 1296790 0.48500177 5.5166416 240-247
ATAAG 791975 0.46177572 5.4007316 9
ACGAT 965795 0.45100528 5.886549 10-14
TAAGG 1039750 0.43377918 5.951772 10-14
CGGTT 1534145 0.43302912 8.158903 10-14
TAAAG 692410 0.4037225 5.934395 9
AAGCT 841700 0.3930556 7.702028 30-34
TGGGT 1521820 0.38375765 5.1251745 10-14
Produced by FastQC (version 0.10.1)