FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_16_SL248345.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_16_SL248345.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7164785
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 8266600 8.550007 60.254013 110-119
CACAG 10984390 7.4017034 42.14836 110-119
ATACA 7532215 7.385838 37.18329 110-119
TCCCT 12998180 7.1475663 109.18988 30-34
CAGTA 9386940 6.671788 40.183342 60-69
AGTAA 7133965 6.2589827 47.522892 110-119
GGAGA 11385495 6.1418486 92.07749 35-39
CCCTG 13079215 6.1008534 71.06874 30-34
GTAAT 7808955 5.854281 39.66998 110-119
GGTGA 12651520 5.8317347 102.4824 45-49
AATAC 5491610 5.384889 39.23389 110-119
GTAGT 9550820 5.189942 25.320766 80-89
AGACG 8447840 5.0932746 100.9041 40-44
ACAGT 7143470 5.0772367 35.846825 110-119
GTGAC 9687910 4.9910207 83.963844 45-49
ACTCC 7434880 4.7845573 125.40981 30-34
GAGAC 7839095 4.7262573 141.48439 40-44
CCCCA 7707250 4.70224 100.83568 60-69
ACCAG 6911245 4.657062 94.29096 45-49
CTCAG 7971365 4.5898266 34.48905 140-149
CACTC 6842895 4.403599 99.753235 25-29
GCACA 6488870 4.372449 34.496677 110-119
GGCCC 11019725 4.3602657 60.071213 60-69
GTGTC 9850990 4.336577 23.767452 120-129
CGTGT 9846020 4.3343887 23.255123 120-129
TAGTA 5751920 4.312146 31.765596 80-89
TGACC 7367420 4.2420816 141.62329 45-49
CTCCC 8120695 4.2335668 71.577034 30-34
AGTAG 6457110 4.106313 28.822092 70-79
ATGCA 5747800 4.085261 98.106346 25-29
GACCA 6004015 4.045735 106.11319 45-49
CCCAG 7254820 3.9602969 86.47129 60-69
TGAGG 8271090 3.81257 69.251816 35-39
CAGGG 8629160 3.7710397 37.394733 50-59
CCACT 5837925 3.7568712 27.36649 240-247
AGGAG 6832060 3.6855202 92.64545 35-39
ATGGT 6759245 3.6729918 25.79426 190-199
TACAC 4613295 3.66466 27.000729 110-119
ACGGT 7087490 3.6513355 94.70674 40-44
GACGG 8344025 3.6464322 107.095695 40-44
TGCAC 6292415 3.6231048 118.18369 25-29
CTGAG 7016640 3.6148348 106.519844 35-39
GGGTT 9090605 3.5805988 44.2497 50-59
AGATA 4044635 3.5485597 18.996634 150-159
TGTCC 7157300 3.5214431 20.1243 120-129
GAGGA 6447565 3.4781067 122.400444 35-39
CCTGA 5998585 3.4539208 85.41296 30-34
GCCCC 7725165 3.416286 73.1516 60-69
CCCTT 6162935 3.388935 31.130491 20-24
CCAGG 6921195 3.3804765 42.319126 50-59
TGGTG 8548990 3.367268 18.123968 190-199
GCACT 5838195 3.36157 90.600266 25-29
TCTTG 6474240 3.3598716 18.56913 170-179
GGATG 7251115 3.3424103 70.9176 20-24
CTTCA 4844995 3.288695 16.84342 200-209
CTCTC 5909740 3.2497056 13.838154 100-109
CCGTG 7730610 3.2264018 23.644693 120-129
CCTGG 7716360 3.2204545 30.191935 50-59
CTTGG 7273530 3.201934 17.636736 20-24
TCAGG 6210510 3.1995327 20.659725 140-149
CAGAT 4478440 3.183061 17.54439 150-159
TGTCT 6071150 3.150684 20.122143 180-189
AGGCT 6104300 3.1448152 21.389803 30-34
TCGCA 5443305 3.1341965 24.238232 100-109
GGTTG 7928095 3.1227105 77.16599 15-19
TTCCC 5667850 3.1166933 51.531906 50-59
CGGAT 6012610 3.0975783 80.56104 20-24
CGCAC 5593725 3.05353 24.530579 100-109
GGTTC 6893495 3.034636 49.866375 50-59
TCTCA 4469420 3.0337615 21.038511 130-139
GATGC 5818870 2.9977674 99.97039 25-29
ACAGA 3594995 2.990258 14.797426 140-149
AGGGT 6475300 2.984798 51.90948 50-59
TGGCC 7069985 2.9506872 44.869457 60-69
ACCAC 3877760 2.9203904 19.767601 230-239
AAAAA 2058315 2.9156513 15.445387 130-139
GTTGG 7396225 2.913218 54.916805 15-19
AGTCA 4007775 2.8485346 33.75567 70-79
TCAAA 2902790 2.8463783 58.795414 70-79
GACTC 4920090 2.8329349 19.548262 190-199
CCAGT 4910270 2.8272808 37.988712 60-69
TCTCT 4839395 2.8069146 15.013392 180-189
CGGTG 7400020 2.7633336 67.38981 40-44
TTCTT 4512130 2.760465 21.026854 170-179
GCCGT 6596325 2.7530034 22.55481 120-129
GCCCT 5875305 2.7405603 17.151608 20-24
GTTCC 5557935 2.734544 56.44486 50-59
CGGCC 6818600 2.6979718 12.859992 120-129
GATGG 5745065 2.6481953 17.646608 15-19
TCACA 3289590 2.6131492 12.077479 140-149
AGATG 4085080 2.5978522 16.562952 180-189
GCGGA 5919995 2.587104 89.48908 20-24
TCCAT 3763465 2.554572 16.510033 70-79
AGGGA 4733205 2.553304 9.16353 150-159
GGCGG 7927295 2.5110693 43.950577 20-24
TGTTC 4769505 2.4751823 12.806315 140-149
ACAGC 3637740 2.4512486 11.186378 120-129
TTGGG 6199475 2.4418433 82.07889 15-19
TCCTC 4426430 2.4340487 16.73886 130-139
GGCTG 6494780 2.4252965 11.803868 140-149
CTGGC 5799935 2.4206266 36.803772 60-69
ACTCT 3529345 2.3956556 13.608903 200-209
GTTCT 4604800 2.389707 17.703989 160-169
TGGAG 5156940 2.3770983 14.39388 25-29
TACCA 2992260 2.3769596 16.32116 230-239
GAGAT 3710550 2.359675 16.249683 180-189
CAGCT 4084520 2.3518229 12.49752 150-159
AATAA 1918305 2.321931 9.029199 110-119
CACGG 4746080 2.3180985 9.676753 120-129
CTGGA 4410340 2.2721202 14.434128 180-189
TCATT 3132915 2.2430627 16.467327 150-159
CATTT 3131125 2.241781 17.47469 150-159
TCAGC 3893330 2.2417376 14.577804 140-149
TGTAG 4117415 2.2374144 8.144991 210-219
CTACT 3294665 2.2363591 7.7340846 180-189
ACTAC 2797090 2.2219224 12.252689 230-239
CACTA 2789325 2.2157543 15.313922 240-247
TTCAT 3066970 2.1958482 15.708457 140-149
TTCAC 3229845 2.1923606 18.29215 200-209
CAGAG 3632935 2.1903276 9.770235 140-149
TGGGG 6495655 2.170297 53.747963 15-19
GTGAA 3410565 2.1689034 17.814425 190-199
TAGTC 3547305 2.154391 28.052532 70-79
GATAC 3002330 2.1339123 14.252017 150-159
TTGTC 4100425 2.1279566 13.001756 170-179
TCTGC 4324305 2.1275892 12.068412 210-219
TTGGA 3884490 2.110842 16.303013 170-179
ATTTG 3267450 2.093136 15.400458 150-159
CTCGC 4478090 2.0888238 16.606396 100-109
GTCAA 2931565 2.083616 42.441624 70-79
TCTCG 4213510 2.0730772 16.959066 100-109
ATATA 1994935 2.063328 10.340302 110-119
ACACA 2199925 2.0451372 13.287369 110-119
GAACT 2867705 2.0382273 11.15528 160-169
AGCTC 3539135 2.0377958 12.544264 140-149
GTCTC 4131215 2.0325875 11.858213 180-189
GCTGT 4610105 2.0294483 10.254085 140-149
GAATC 2854515 2.0288525 14.176508 190-199
TACAG 2852660 2.0275342 13.76445 160-169
CTCTG 4117475 2.0258274 14.464457 180-189
GATAT 2694900 2.0203345 8.303138 180-189
ATCTC 2964245 2.0120761 8.797349 100-109
AACCA 2157720 2.0059016 15.280885 70-79
GTCCT 4057525 1.9963315 14.137375 130-139
GTTCA 3282655 1.9936608 14.826024 140-149
TGAAT 2642565 1.9810996 13.831693 190-199
AATAT 1910500 1.9759983 12.847592 110-119
CAGGC 4042370 1.9743899 15.252071 140-149
ACACG 2907610 1.9592592 13.332333 120-129
AATCG 2732790 1.9423364 12.933129 190-199
ATGTA 2590405 1.941996 7.019715 150-159
TTGCA 3193630 1.9395931 14.174597 150-159
GGGGC 6107065 1.9344888 49.020023 15-19
CCTTC 3511205 1.9307758 14.05176 200-209
ACGGC 3952375 1.9304342 14.481715 120-129
TACTA 2288605 1.9175901 9.435932 230-239
ATATG 2544600 1.9076564 8.474967 180-189
GAGTC 3697605 1.9049333 12.18881 210-219
CAGCC 3480755 1.9000918 10.345514 120-129
TGGAA 2986480 1.8992122 9.3393755 170-179
TTTGC 3640800 1.8894296 12.208594 150-159
GGGCG 5963990 1.889168 62.067684 20-24
CATAG 2631105 1.8700634 8.204804 210-219
TCGGC 4477305 1.8686215 7.859977 130-139
AGTAT 2423635 1.8169705 11.823652 220-229
GTTGT 3831420 1.7790551 7.1371837 210-219
CTGTT 3427465 1.7787169 12.687043 140-149
AGAAC 2130240 1.7718985 11.7406645 160-169
GACGT 3406550 1.7549875 10.039023 170-179
GTCAC 3022090 1.7400869 8.875103 140-149
CGTAG 3371275 1.7368146 9.414906 210-219
CTCCA 2677520 1.7230605 7.795486 150-159
ATTGT 2684460 1.7196712 7.632038 170-179
GCAGA 2843005 1.714072 14.306124 150-159
AGTCT 2811700 1.7076348 12.950724 210-219
TGGTT 3663720 1.7011864 18.547405 10-14
TGCAG 3289960 1.6949228 13.505198 150-159
CACCA 2247060 1.6922895 13.255533 230-239
GGTCA 3275695 1.6875737 7.7745976 130-139
ATAGT 2244240 1.6824801 7.369265 220-229
AACTG 2349730 1.6700755 11.035381 160-169
CCAGA 2478155 1.6698756 24.016129 60-69
TTCAG 2737200 1.6623888 9.667192 150-159
TGACT 2735390 1.6612895 11.825108 190-199
GTCTG 3767785 1.6586443 9.05573 210-219
CAAAA 1438650 1.6509135 20.479895 70-79
TCCCA 2552370 1.6425229 10.347325 240-247
GAGAA 2198965 1.6365314 10.829526 160-169
CAAAG 1940320 1.6139262 30.157312 70-79
ACTGA 2257440 1.6044803 9.016009 180-189
TCAGA 2256675 1.6039366 9.850876 130-139
CTGGT 3637375 1.6012355 7.2772746 160-169
GTGCT 3634840 1.6001196 8.312695 220-229
TTGTA 2497340 1.5998018 9.085244 210-219
TCTGG 3625580 1.5960432 11.93721 180-189
TGTGC 3619685 1.5934482 8.072628 220-229
TAGTT 2482060 1.5900134 10.058437 220-229
GCTCC 3396095 1.5841227 6.2159805 150-159
AATAG 1795895 1.5756282 9.502257 240-247
TGCTC 3201545 1.5751832 5.1123276 150-159
ATAGA 1781565 1.5630556 10.1048 240-247
CCTTG 3176755 1.5629863 20.041666 20-24
GAACC 2313355 1.5588272 12.819833 70-79
GGACG 3564600 1.5577701 7.5419927 170-179
CTGTG 3523750 1.5512158 5.168722 210-219
GGCCG 4375635 1.5490985 13.34245 120-129
TTGGT 3328470 1.5455188 21.977324 25-29
GCTCA 2669070 1.5368218 11.210779 140-149
CCCAC 2486665 1.5171294 10.279612 240-247
GTCCC 3239905 1.5112672 18.34589 50-59
CCACC 2459190 1.5003668 9.664672 230-239
ACCAT 1870105 1.485554 11.001044 45-49
GCTCT 3009280 1.4805874 9.960573 130-139
CCATC 2234935 1.4382446 10.437149 240-247
CGACT 2492955 1.4354167 8.714591 190-199
TGATA 1914165 1.4350268 7.823388 180-189
CCTCG 3074935 1.434316 9.518258 130-139
TATAC 1705075 1.4286584 6.632865 110-119
TGGAC 2761730 1.422789 10.684461 170-179
AATTG 1897370 1.4224358 9.451327 170-179
CACTG 2463575 1.4185002 7.4085417 230-239
CTCTT 2445440 1.4183884 8.481052 200-209
TCGAC 2438975 1.4043356 9.4383 190-199
GGGAG 3584915 1.401739 6.6199617 150-159
GAGGG 3582340 1.4007322 8.344206 200-209
CTTGA 2260350 1.3727825 8.701778 200-209
CTGCT 2789650 1.3725276 6.0446525 90-99
CATGT 2229630 1.3541253 6.6756797 150-159
GCTGA 2623310 1.3514777 18.426622 30-34
GCGTA 2614835 1.3471116 9.969865 210-219
CAGAC 1996425 1.3452677 13.690051 60-69
ACGGA 2222575 1.3400096 9.158019 200-209
CTGAT 2205455 1.3394428 7.520654 180-189
CCTCA 2065995 1.3295268 7.2557526 130-139
GAATT 1759365 1.3189751 10.430002 170-179
CTCGA 2290670 1.3189431 8.469989 190-199
AGATC 1853715 1.3175318 6.981745 130-139
ACTTC 1930745 1.3105549 6.498759 220-229
GGAAT 2042595 1.298961 8.7853985 170-179
TCCAC 2017405 1.2982578 9.529282 240-247
ACTAT 1549420 1.2982371 6.365155 230-239
TATGG 2376145 1.2912035 6.2215123 190-199
ACGTG 2506175 1.2911321 9.530565 170-179
ATCGG 2487805 1.2816683 7.697962 190-199
AAAGT 1459045 1.2800926 22.4147 70-79
ATAAC 1298865 1.2736236 7.9572587 80-89
AGCCG 2594390 1.2671618 9.160796 120-129
GTGTT 2727020 1.2662456 7.557292 160-169
GGAGT 2741480 1.2636888 7.0299296 210-219
AAGTA 1431410 1.2558472 16.25784 70-79
GTCCA 2172310 1.2507927 15.428398 70-79
ACTCG 2148260 1.236945 8.704849 190-199
GGCTC 2957610 1.2343707 8.221946 130-139
CCATG 2131245 1.227148 6.7662573 70-79
GTGGA 2658685 1.2255243 18.69968 25-29
ACTGG 2373615 1.2228398 7.442557 160-169
TTGAG 2244530 1.2196836 8.323595 200-209
TTCCA 1780975 1.2088938 7.863789 230-239
AGCTT 1983285 1.204512 6.5014467 150-159
ATAAA 994575 1.2038412 5.3618126 7
GATCT 1980075 1.2025626 5.4247828 130-139
AGAGC 1982170 1.1950672 7.43333 140-149
GGAGG 3017430 1.1798464 14.397085 30-34
ACCGT 2046595 1.1784074 16.055748 45-49
GCATA 1655620 1.1767354 7.9751945 210-219
TATCA 1385400 1.1608073 6.645722 70-79
TCACG 2007980 1.1561733 9.4414215 200-209
GTGGT 2931080 1.154491 9.44025 50-59
TTGGC 2615490 1.1513841 7.6473465 60-69
ACTGT 1884660 1.144614 5.5732765 230-239
ATACC 1431635 1.137247 6.6939425 70-79
TGGTC 2579375 1.1354856 10.731399 50-59
TCTAC 1663730 1.1293099 7.5668564 180-189
AAAAG 1095875 1.12519 13.584256 70-79
ATATC 1339040 1.1219628 7.1093817 70-79
TGCCC 2402425 1.1206212 6.0106397 60-69
GTCTA 1838205 1.1164004 7.6654806 180-189
GCTTC 2266500 1.1151342 5.85088 150-159
AGGTT 2042540 1.1099216 23.311903 10-14
TCTGT 2110685 1.0953611 6.3923573 210-219
TACTG 1798720 1.09242 6.7610526 180-189
CAATA 1113085 1.0914539 6.62399 100-109
GAGCT 2114970 1.0895909 6.5572205 140-149
AGTTC 1790805 1.0876129 5.2620745 160-169
GAAGA 1460900 1.0872428 17.396055 35-39
ATCCA 1366455 1.08547 15.007416 240-247
CCATA 1362905 1.08265 9.275207 70-79
CGGCT 2574985 1.0746804 7.777924 130-139
CTACC 1669580 1.0744224 8.826811 230-239
AGGGG 2742895 1.0725005 5.0379066 60-69
CTGAA 1507340 1.0713451 13.866844 35-39
CTCGG 2561815 1.0691841 8.34487 130-139
TATGT 1665960 1.0672178 7.366922 220-229
GGGGT 3189265 1.0655817 7.9996843 10-14
CTGCG 2539195 1.0597434 6.751631 210-219
AAAAT 865450 1.0475472 5.285058 8
GCGGT 2770815 1.0346845 5.914324 130-139
GTCCG 2475110 1.0329974 5.2071357 130-139
CTGCC 2213535 1.0325125 5.6504164 200-209
TAGAT 1369895 1.026994 7.8007007 240-247
CTTCT 1743315 1.0111463 5.3277993 210-219
ATACG 1421620 1.0104195 5.1977077 120-129
CATGG 1958790 1.0091301 5.1413054 190-199
TATAA 970665 1.0039426 6.5174613 240-247
CAGCG 2047270 0.9999355 6.4354715 160-169
AGAGA 1342845 0.999383 12.214607 35-39
TGCGT 2245385 0.98845744 8.054147 210-219
AACTT 1172505 0.98242533 5.672127 200-209
CGAAC 1456125 0.98119277 13.567842 70-79
ACCCA 1284530 0.96739584 12.6483 240-247
ATGGG 2098240 0.96718645 27.145878 15-19
CGGAG 2207890 0.96487266 6.6113396 210-219
AGCTG 1865560 0.96109986 5.159868 90-99
TGTAA 1259260 0.9440523 6.8903713 230-239
TCCCC 1809055 0.9431158 5.0681047 80-89
AAGAG 1258780 0.93681943 12.856107 35-39
ACAAT 952770 0.9342542 7.6345224 100-109
ACTAA 948135 0.9297093 12.246961 240-247
TAGGC 1770330 0.9120392 5.5938945 160-169
TTCTG 1745840 0.9060212 5.407533 210-219
CCATT 1331735 0.90395766 5.4790382 50-59
TCGAA 1269010 0.90195155 16.998083 70-79
CCCAA 1194185 0.8993559 9.76847 240-247
TGCAT 1462980 0.8885143 6.0728364 210-219
TGAAC 1247380 0.8865781 6.4496694 190-199
CATCA 1114680 0.8854675 5.6337395 230-239
CGTGG 2343955 0.87528545 6.5263295 50-59
CCAGC 1586420 0.86600274 5.7980466 80-89
AGCGT 1676125 0.8635066 6.6023016 160-169
CTTCC 1564975 0.86056376 5.5364437 230-239
TGAAG 1351060 0.8591887 9.129244 35-39
CGGTC 2057805 0.8588334 5.7278495 130-139
GTATG 1578795 0.8579214 5.229124 220-229
CCAAT 1070230 0.85015786 10.559942 240-247
ACGTC 1457010 0.8389308 10.022179 70-79
ATCAA 836935 0.8206705 12.209013 70-79
GGTGG 2441605 0.81577724 18.914282 25-29
TAACC 1018700 0.809224 6.521027 80-89
CGTCC 1729210 0.80659723 8.880898 70-79
AAAGA 771695 0.79233813 6.201582 8
GAGGC 1799665 0.78647375 12.178766 30-34
AACCG 1145350 0.77178067 5.5660696 190-199
AAAGG 1027935 0.76501805 8.791111 10-14
TGAGA 1197295 0.761404 5.349855 240-247
GGGCC 2127065 0.75304115 14.337599 15-19
ATGAT 994410 0.7454974 7.146234 240-247
AGCCA 1104945 0.7445542 5.7975335 80-89
CTAAT 884750 0.7413195 9.465537 240-247
GTCGA 1423030 0.7331171 12.405668 70-79
GACCG 1487240 0.7264034 14.954257 45-49
AATCC 912715 0.7250328 9.235427 240-247
GTAGC 1380775 0.7113483 5.550304 80-89
AAGGT 1114875 0.7089899 10.078423 10-14
GGTCC 1680135 0.7012112 10.986775 50-59
TGGGC 1872225 0.69913083 15.565915 15-19
GGAAG 1263275 0.6814673 5.9336953 30-34
CATAC 857860 0.68145764 7.884955 70-79
AAAGC 792500 0.6591884 9.207712 70-79
TATGA 869415 0.65179014 5.769007 240-247
TAGGT 1194720 0.649214 5.486639 10-14
GAAAA 621345 0.6379663 5.8540907 8
CAATC 796025 0.6323378 8.987425 240-247
ATAGG 973690 0.6192052 5.3354416 10-14
GAGGT 1319430 0.608193 6.5989084 10-14
GGGTC 1621890 0.6056501 6.099877 70-79
AGAAG 806715 0.60038 5.419315 9
AATGG 921040 0.5857231 6.75188 10-14
TAAAA 482150 0.583598 5.7888966 8
AAGAA 561930 0.5769619 5.5002584 8
AAATG 652900 0.5728216 5.260691 9
CGATG 1087510 0.5602638 12.252499 15-19
CATCC 864535 0.5563529 6.809355 240-247
AAGGG 989195 0.5336163 6.5694566 10-14
GAAGG 988055 0.53300136 5.612957 10-14
AAGCA 615795 0.5122081 6.189834 70-79
TGATC 835920 0.50768083 5.4351683 240-247
GGTCG 1357095 0.5067697 6.178549 70-79
ATAAG 550045 0.48258188 5.976927 9
GATCC 828800 0.47721413 5.4360404 240-247
CGGTT 1031310 0.45400056 8.095551 10-14
TAAGG 700835 0.4456867 6.154237 10-14
ACGAT 605630 0.43045285 5.4612474 10-14
TAAAG 464195 0.4072614 5.184843 9
AAGCT 554090 0.39382064 7.5369654 30-34
Produced by FastQC (version 0.10.1)