FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_12_SL248341.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_12_SL248341.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9945621
Filtered Sequences 0
Sequence length 251
%GC 54

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 45797 0.46047401162783097 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 35870 0.36066123975566733 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 35151 0.353431927478435 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33575 0.33758575759120524 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 31954 0.32128712726937814 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 31793 0.3196683243811523 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 31664 0.31837127113530667 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29983 0.30146936023401655 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 28829 0.28986626375567703 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 28701 0.2885792651861558 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27483 0.2763326694230556 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26361 0.26505132258709635 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25244 0.253820249132759 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25040 0.2517690951625846 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24430 0.24563574260471013 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24185 0.24317234690523598 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24100 0.2423176994176633 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24067 0.24198589509895863 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23747 0.23876839867515562 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23509 0.23637538570995217 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22769 0.22893492522990772 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22408 0.22530518707680494 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22326 0.22448070361820543 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21691 0.21809598415222137 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21330 0.21446624599911862 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21202 0.2131792474295974 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21050 0.211650936628291 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20892 0.21006229776903826 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20697 0.2081016358857833 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 20222 0.2033256646317007 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20035 0.20144544015904087 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19665 0.1977252099190186 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19034 0.1913807091583321 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18903 0.19006354655983776 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18372 0.18472451343158966 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17811 0.17908384001361002 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17762 0.17859116087371515 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 17620 0.1771633968356526 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 17532 0.17627858531910678 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17516 0.17611771049791664 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17473 0.1756853594159681 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16993 0.1708591147802636 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16825 0.16916992915776702 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16738 0.16829517231754557 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 16714 0.16805386008576034 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 16307 0.16396160682173594 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16114 0.16202105429112973 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15555 0.15640049022579888 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 15309 0.1539270398500003 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15103 0.15185577652717713 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 14763 0.14843718657688645 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14494 0.14573247864562705 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 14212 0.14289705992215068 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 13910 0.13986054767218659 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13785 0.13860371313163855 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13772 0.13847300233942153 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13762 0.1383724555761777 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13695 0.13769879226244394 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13690 0.13764851888082202 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13431 0.13504435771280648 No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG 13284 0.133566320293122 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12598 0.1266688123345943 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12124 0.1219028957568361 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12091 0.12157109143813143 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12022 0.1208773187717489 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11198 0.11259226548045619 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 10375 0.10431726686548784 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10363 0.10419661074959523 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10172 0.10227616757163781 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 10170 0.10225605821898903 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 20037020 7.615743 65.68844 35-39
TATTA 9904595 7.220462 124.856155 240-247
TGGAT 15192940 7.1580343 78.11405 35-39
TCACC 15512225 6.3865123 73.82695 160-169
TCTCC 13691275 6.0633593 85.78677 20-24
CTGTG 14448320 5.9071264 86.06011 25-29
TCCCT 13145805 5.8217907 73.18421 15-19
AGTGG 15613540 5.701974 39.51966 100-109
CTGAG 14637570 5.5635014 49.30025 15-19
TGTAT 9015860 5.480083 58.872517 240-247
TTACT 8394890 5.3106813 111.1573 240-247
ATTAC 8427850 4.9564676 104.001816 240-247
TGGGT 12420225 4.879026 44.989136 100-109
CCTGA 12181740 4.818853 50.87836 15-19
CTCTG 11113360 4.7288938 73.22967 35-39
TTCAC 9027560 4.607155 73.68409 40-44
ACACG 12414620 4.5654974 48.512684 230-239
GACAC 12401130 4.560536 45.834988 230-239
CTCCA 11040015 4.545266 40.428528 80-89
TGAGA 9984730 4.3732924 92.28973 15-19
TCTGG 10505325 4.295052 134.68349 35-39
TACTG 8755920 4.2934747 78.44288 240-247
ATTCA 7230110 4.25207 109.56599 40-44
AAGGG 12492105 4.241112 38.914032 90-99
CCCTG 12159825 4.1741557 85.226204 10-14
TGGAG 11374575 4.153929 38.64928 90-99
CAGCC 12860115 4.1039915 54.729836 30-34
AGCCT 10370250 4.1022635 50.503456 30-34
GAAGG 12071880 4.0984445 30.752731 90-99
CCAGG 13308575 4.0807304 52.659885 80-89
ACCAT 8508295 4.0366883 66.51417 160-169
TCAGT 8162690 4.0025845 94.711845 7
GAGAC 11286065 3.9878786 88.38283 8
ACTAC 8314810 3.9448912 35.270393 130-139
CACCA 10269600 3.930641 56.719654 160-169
TCCAG 9886005 3.9107063 39.936893 80-89
GTATT 6419480 3.9019332 68.31802 240-247
GGGTC 12238930 3.878594 123.80001 8
TACTA 6590195 3.8757317 28.301529 130-139
CACCT 9218950 3.7955182 44.791763 20-24
CTGGG 11854925 3.7569003 131.05374 3
ACTGT 7651990 3.7521622 67.665665 240-247
TGAAG 8460725 3.7057815 38.960247 150-159
GACTC 9356720 3.7013314 51.13335 20-24
GATTC 7481545 3.6685839 58.131393 40-44
AGACT 7983910 3.639511 67.52658 15-19
CAAGA 8469295 3.5891771 43.585953 180-189
GGACA 10136210 3.5815825 27.999275 220-229
GCTGG 11269075 3.571241 32.962734 90-99
ACTGG 9276115 3.5256994 44.09642 60-69
CCTGT 8239730 3.5061224 61.068623 25-29
GAGTG 9387380 3.4282165 38.690357 90-99
ACTCT 6664590 3.401229 67.21649 20-24
TCCTG 7793925 3.3164268 63.33376 20-24
TATCT 5215520 3.2993839 47.990536 200-209
GGATT 6927940 3.2640443 55.672718 40-44
CTCCT 7339495 3.2503908 92.62286 20-24
AGCTG 8531685 3.2427533 36.722424 60-69
AGACA 7633160 3.234834 37.300663 170-179
CTGCA 8108110 3.2074068 46.075512 25-29
GGTCC 9719050 3.2056043 140.8358 9
CATCT 6153175 3.1402316 32.345158 170-179
GTCTC 7321025 3.1152008 33.759056 30-34
CCATC 7538895 3.1038256 35.42028 45-49
GTCCC 8938065 3.0682087 73.85403 10-14
CCTCA 7385395 3.0406284 80.050766 5
GGAGT 8197485 2.993674 36.986217 90-99
GGCTG 9219740 2.9217937 34.08329 90-99
AAGAA 5952725 2.907077 37.005325 180-189
AGGGA 8544165 2.9007728 39.75555 80-89
AGTAG 6544035 2.8662746 28.545927 50-59
GAGCT 7479310 2.8427632 29.695942 60-69
GTGTA 6021240 2.8368602 35.90915 230-239
TGTGC 6851630 2.8012557 57.02582 25-29
ATCTC 5477940 2.7956297 47.30326 170-179
GTGAA 6302450 2.7604609 38.36126 10-14
GGAAG 8122290 2.7575452 29.862976 80-89
ATCTG 5557600 2.7251751 36.02755 200-209
CAGGG 9244465 2.7235346 35.992573 80-89
GACAA 6326275 2.680993 24.11603 180-189
ACCTT 5214035 2.6609476 70.68017 40-44
CTATG 5411605 2.6535866 27.41971 50-59
GAACA 6227510 2.6391377 34.170002 210-219
GGGAA 7745025 2.6294622 33.503036 80-89
GAGTC 6800330 2.5846937 28.251823 150-159
ATGAA 4918950 2.5840023 27.040436 210-219
CCCTC 7196000 2.570915 27.572826 20-24
CACGG 8321845 2.5516784 43.76825 230-239
CGCCA 7902305 2.5218277 39.320904 70-79
AGAAC 5909545 2.5043883 29.706774 190-199
TGCAA 5489050 2.5022151 34.92274 200-209
CAGTG 6572655 2.4981585 73.37073 8
ATCAG 5474670 2.4956596 27.33041 45-49
AGGGC 8444130 2.4877458 32.362736 150-159
CTACA 5173850 2.4546893 19.22631 190-199
CCAAG 6652335 2.4464073 38.400646 180-189
AGGCT 6412875 2.4374287 20.046288 25-29
ACGGC 7921065 2.4287896 48.41866 230-239
GTGGA 6643795 2.4262757 26.900497 100-109
TATAT 3309310 2.412491 23.475952 240-247
CTGTC 5657165 2.4072046 49.289314 30-34
TCTGT 4563585 2.4070942 24.97906 210-219
CACTG 6025165 2.383435 19.877367 30-34
CTACT 4628700 2.362226 23.048306 50-59
GCAGC 7686270 2.3567958 33.93867 30-34
CCGCC 8488740 2.350779 33.802803 70-79
CTCTC 5274465 2.3358655 96.309975 20-24
TGAAC 5103920 2.3266513 37.213776 210-219
GTATC 4731285 2.319991 22.54404 200-209
GTGGT 5856530 2.3006153 30.883993 120-129
ACAGC 6250400 2.2985947 26.368093 210-219
GGGCT 7248910 2.2972252 42.09897 90-99
CTTCA 4494295 2.2936335 50.27283 45-49
TGGTG 5833350 2.2915096 34.880672 35-39
TATGC 4664690 2.2873359 21.077991 50-59
TCCAA 4776590 2.2662127 42.34395 180-189
CAAAT 4122150 2.2537174 34.470016 200-209
GGCCG 8799545 2.2496672 25.122059 230-239
CAGGC 7271810 2.2297125 32.873707 70-79
TATCA 3770530 2.2174706 17.09642 170-179
GCCTC 6458430 2.2170136 70.97174 4
ACAAC 5005065 2.2075608 19.552872 180-189
GCTCC 6377490 2.189229 33.47264 80-89
CAGTA 4788100 2.182683 21.143147 45-49
AATGA 4148005 2.1790128 25.402416 210-219
GCCTG 6605600 2.178705 30.587973 210-219
AAATG 4121800 2.1652467 32.723186 200-209
TACAT 3655410 2.1497676 17.924213 190-199
GGGGG 9095900 2.1468081 93.69936 5
CCTTC 4823075 2.1359613 54.945786 45-49
AAAAA 3625390 2.1234584 14.94697 90-99
GTGGG 6948360 2.1157188 29.651594 100-109
GTAGT 4466745 2.1044717 24.760742 120-129
GCTAT 4244675 2.0813816 33.5307 50-59
TCTAT 3283640 2.0772595 20.281218 110-119
TGCAG 5446215 2.0700173 42.85996 30-34
CCTCT 4672600 2.0693214 48.266403 35-39
GCAGA 5854270 2.068579 23.976992 140-149
GCTGT 5049405 2.064425 19.167902 230-239
CTCAC 4983160 2.0516086 55.913315 20-24
AGAGT 4624675 2.0255985 30.474192 150-159
TGCAC 5104410 2.019203 27.110708 25-29
TGTGA 4251915 2.0032563 19.134518 210-219
AACTA 3657225 1.9995275 36.481968 130-139
AACAG 4717655 1.999281 32.725895 210-219
AGCAC 5384950 1.9803241 20.487665 130-139
CAGAG 5565510 1.9665473 31.78469 170-179
TGAGC 5172620 1.9660281 17.807856 200-209
GGCTC 5958495 1.9652721 20.48453 40-44
TCTCT 3571435 1.9605817 33.229908 35-39
ATGGA 4440590 1.9449697 12.023199 100-109
ATATC 3306400 1.9445131 13.61789 160-169
TCCGC 5652905 1.940497 41.69613 70-79
TGGTA 4112840 1.9377323 30.653664 120-129
CATAT 3277535 1.9275374 14.41867 160-169
CCGTG 5831000 1.9232209 20.050076 230-239
GGTGG 6312475 1.9220971 18.564312 40-44
TTCAG 3916790 1.9206023 46.917698 45-49
GGTCT 4677765 1.9124818 35.477398 15-19
CAGAC 5200450 1.9124742 26.046974 140-149
CTGTA 3868035 1.8966954 23.934654 190-199
GGATC 4975415 1.8910735 18.363396 70-79
GCCAG 6113610 1.8745805 35.095627 70-79
ACCCT 4546085 1.8716608 44.465706 10-14
TGGGG 6146475 1.8715514 121.92605 4
TCCAT 3659085 1.8673896 27.256224 45-49
CCTGC 5424500 1.8620914 49.699028 20-24
GCACA 5055060 1.8590068 18.082151 130-139
GTCAC 4698935 1.8588051 37.967255 160-169
ACCAG 5052725 1.8581481 13.945452 190-199
CCAGA 5037710 1.8526263 33.294174 170-179
ACTAT 3139475 1.8463435 16.976517 130-139
CTATT 2912595 1.8425332 19.487562 110-119
CAGTT 3747595 1.8376375 24.793327 190-199
TGTCT 3482070 1.8366417 50.49315 30-34
TGGGA 5024870 1.8350537 16.52915 100-109
GATGG 5006470 1.8283336 20.775255 100-109
AGAGA 4479840 1.8241264 27.467703 170-179
GCTGA 4794075 1.8221494 26.965012 200-209
AGGAC 5150690 1.8199723 20.3511 220-229
TCAGC 4564515 1.805631 11.828077 45-49
TTCCA 3518435 1.7956096 22.485506 180-189
CACAG 4871530 1.7915133 15.689077 140-149
GAGGA 5212175 1.769551 26.561035 220-229
GCCGA 5761500 1.7666152 19.053791 220-229
TAGTG 3744415 1.7641517 26.025846 120-129
ATACT 2993590 1.7605476 26.272919 130-139
ACCTG 4447145 1.7592018 31.096497 25-29
TACAA 3205765 1.7526991 17.934376 130-139
AGTCA 3839625 1.750315 28.984335 160-169
CAAGG 4951440 1.7495682 16.997229 80-89
TAGTA 3088920 1.7454479 16.772907 120-129
AGTTC 3529845 1.7308635 21.958553 190-199
CTGAA 3767770 1.7175597 23.33589 200-209
TTCTG 3255355 1.7170591 42.584835 30-34
TGACC 4324890 1.7108401 14.502095 210-219
CCTGG 5169325 1.7049824 34.333237 2
GCAAA 4018120 1.7028272 31.390612 200-209
CATGA 3703570 1.6882937 22.817572 60-69
GGAGC 5715725 1.6839237 13.05635 200-209
CTCAG 4234355 1.6750263 76.57039 6
GTGCA 4399415 1.6721456 49.718433 25-29
AGCTA 3651275 1.6644546 25.398502 50-59
ATGCA 3637130 1.6580065 16.185802 60-69
ATTGG 3506080 1.651862 20.683006 100-109
GGATG 4519390 1.6504549 22.166946 100-109
GCCGT 4988840 1.6454539 29.28402 230-239
AACAC 3721665 1.6414974 22.018461 190-199
AGGGG 5689010 1.6103956 32.58066 90-99
TCTCA 3129265 1.5969994 25.783083 100-109
TCTGC 3736150 1.5897852 32.89846 200-209
CAGCT 4016660 1.5889105 12.8668785 50-59
GGGGC 6452615 1.5850339 28.506441 90-99
GGGCC 6166185 1.5764297 27.734486 2
CTCCC 4406300 1.5742389 13.907916 190-199
CGATT 3196315 1.5673168 34.629967 160-169
TCTGA 3188325 1.5633988 21.03587 220-229
GTTCT 2944260 1.55297 23.273207 190-199
GCACT 3909450 1.5465003 26.077665 25-29
TTCTC 2815065 1.5453632 22.048424 190-199
CGTCC 4501505 1.5452514 19.122288 180-189
AGTTA 2695840 1.5233313 15.949815 50-59
ACAAT 2781160 1.5205534 22.476862 180-189
GGCTT 3709400 1.5165704 21.243183 25-29
CCATG 3799745 1.5031033 15.488116 160-169
GAGCA 4249310 1.5014739 7.571395 200-209
CGGCC 5626370 1.4970682 31.415472 230-239
CTGGT 3659915 1.4963387 32.12046 35-39
AGTGA 3413190 1.4949704 84.16847 9
CATCA 3128270 1.4841813 31.589642 45-49
GTCCG 4482470 1.4784395 33.913303 70-79
GTTAC 3000230 1.4711661 16.030308 50-59
TTAGT 2402335 1.460204 24.99717 110-119
CTTTA 2298160 1.4538362 41.44644 45-49
AAGAG 3506390 1.4277515 18.961243 150-159
TACGC 3609140 1.4277037 24.257734 140-149
GTGAC 3753315 1.4265735 15.243489 210-219
ACTCC 3463215 1.4258345 16.807459 140-149
CGTGT 3459630 1.4144531 23.808073 230-239
CTCAA 2979840 1.4137598 18.767786 150-159
GGCAG 4735490 1.3951343 13.418648 150-159
GTCCA 3526620 1.3950607 28.742395 180-189
CAACC 3628160 1.3886611 15.404014 140-149
GGGGT 4554295 1.3867455 116.42983 7
AACCC 3596300 1.3764669 20.329292 140-149
TAGCA 2996760 1.3660902 11.45854 130-139
CAACT 2869380 1.3613529 17.577805 130-139
CTACG 3440490 1.3609892 16.094944 130-139
AAGCT 2982085 1.3594006 16.795994 200-209
AGCAG 3833285 1.3544734 12.61805 50-59
GGCCT 4080260 1.3457797 67.98297 3
ATCTA 2286665 1.344801 20.815119 110-119
GAGAG 3956005 1.3430772 25.218843 220-229
ATACA 2429295 1.328177 21.543938 35-39
CACGT 3352405 1.3261445 16.690065 180-189
CGACA 3597500 1.3229866 15.994114 70-79
TGTCC 3105940 1.3216219 55.081486 15-19
GAACT 2891650 1.3181752 14.165475 190-199
TCAAG 2888450 1.3167166 23.352604 150-159
CCATA 2768460 1.3134723 14.757478 160-169
TTATT 1673270 1.3121214 20.484804 240-247
ACAGT 2877345 1.3116542 12.286344 120-129
GTGCG 4132115 1.3094933 17.274412 240-247
GTGGC 4120455 1.3057982 14.735485 40-44
TTACA 2215145 1.3027397 9.780302 120-129
TGGAA 2974110 1.3026544 18.955313 120-129
TGTGT 2553250 1.2939724 14.900364 240-247
AGAGC 3658265 1.2926309 24.05611 220-229
CAATT 2190425 1.2882016 23.556187 180-189
ACAGA 3037480 1.2872446 19.820122 140-149
AACCA 2914865 1.2856458 17.320856 190-199
ATCAT 2184820 1.2849053 15.707733 110-119
TCACT 2516960 1.2845137 13.205394 190-199
ACATG 2811110 1.2814605 14.087731 190-199
CCTTT 2333815 1.2811754 47.472073 45-49
TCCGT 3001415 1.277145 13.621561 150-159
AGACC 3470385 1.2762398 101.554306 9
TGCGA 3347590 1.2723641 14.433122 240-247
CCAGT 3215865 1.272132 18.932209 190-199
CCTAC 3079485 1.2678494 16.980215 190-199
ATTAT 1722150 1.2554494 9.440336 50-59
AATTC 2128740 1.2519244 23.525974 180-189
ACACA 2836020 1.2508701 7.6563373 130-139
ATCCG 3154860 1.2479997 24.057085 70-79
GATTG 2647665 1.2474265 23.966444 100-109
ACATA 2272945 1.2426952 20.41094 130-139
ACGTC 3133880 1.2397003 18.460484 180-189
ATATT 1697035 1.2371405 25.121046 240-247
TCGGA 3244890 1.2333295 95.159325 5
AGTCG 3240720 1.2317444 16.19724 150-159
CTTCT 2243480 1.231585 23.12019 30-34
CGGCT 3696180 1.2190996 21.015331 230-239
GATCT 2468570 1.210466 9.36742 220-229
GTCGA 3141015 1.1938484 12.705138 150-159
TGGAC 3133695 1.1910661 15.680733 80-89
TACCA 2507005 1.1894273 10.83278 45-49
TGAGT 2513130 1.1840415 20.62717 90-99
AGATC 2595600 1.1832191 12.337582 210-219
GATCC 2989235 1.1824816 23.884876 70-79
CTTCG 2768215 1.1779151 107.48595 3
CGCTG 3532290 1.1650444 12.288708 190-199
GGGTG 3793570 1.1551108 14.488922 60-69
ATTAG 2043425 1.1546729 24.687342 110-119
AAGGC 3265455 1.1538333 28.276384 25-29
TCGAG 3012395 1.1449621 12.366015 150-159
GAAGT 2611210 1.1437048 13.390365 140-149
ACGCA 3101140 1.1404494 20.626417 140-149
AGCTC 2879665 1.1391379 12.478205 210-219
GACCC 3568465 1.1387883 43.57524 10-14
TTGGG 2884015 1.1329249 15.427509 100-109
GGACT 2978615 1.1321228 13.128613 90-99
AGGCC 3689155 1.131184 14.165558 2
GAACC 3067955 1.1282455 10.569978 180-189
ATTCC 2200255 1.1228853 21.966572 180-189
TTCGG 2735205 1.1182753 103.3359 4
GCCCC 4016900 1.1123965 12.423157 80-89
AGGTC 2904015 1.1037683 17.788168 10-14
ACAAG 2600130 1.1019014 17.087519 80-89
GTATA 1949420 1.1015538 10.046064 240-247
GCAAG 3102605 1.0962912 16.703548 25-29
GCACC 3426170 1.0933784 11.261785 130-139
GCTTC 2569450 1.0933377 28.551123 2
CGCAG 3561270 1.0919714 16.277342 140-149
CCCCT 3041145 1.08651 9.738965 140-149
TAGAC 2381805 1.0857595 17.426321 170-179
GAAGC 3055050 1.0794877 13.099246 200-209
CCGAT 2719980 1.0759698 23.15157 160-169
CACAT 2266570 1.0753549 13.536825 130-139
TTGAG 2279120 1.0737894 20.378664 90-99
CTTGA 2187920 1.0728492 21.84537 90-99
GTAGA 2436575 1.0672151 17.200506 170-179
GGAGA 3136780 1.0649474 84.75081 7
ATGGG 2915545 1.0647401 19.733673 100-109
TACAG 2323415 1.059142 9.209283 120-129
CAGCA 2871660 1.0560578 12.588831 45-49
ACACC 2752730 1.0535945 13.005708 40-44
TGGCT 2572010 1.051554 31.958582 40-44
ATCAA 1917790 1.0485201 12.367325 110-119
GGGAG 3672130 1.0394746 14.740881 5
CTCCG 3026645 1.0389695 9.777219 140-149
GCTCT 2435320 1.0362636 17.505968 210-219
CTATA 1761905 1.0361866 12.990791 50-59
ATGAG 2355715 1.0317986 19.688347 60-69
ATGGT 2185965 1.0299002 9.622696 35-39
ACCGC 3209000 1.024074 11.143727 220-229
GAGCC 3330275 1.0211427 17.67774 220-229
GACAG 2866440 1.0128433 15.399503 70-79
TAGTT 1665235 1.0121747 14.874326 50-59
CGTGA 2662835 1.0121 20.201012 150-159
GTAGC 2640570 1.0036374 11.129456 50-59
AAACT 1823675 0.99706423 16.810371 130-139
TTTCA 1569125 0.9926423 7.303068 100-109
CGAGG 3369230 0.99261713 14.409924 220-229
TGAGG 2716395 0.99201185 11.78765 220-229
AGCCG 3230010 0.99039894 18.69123 220-229
TTATA 1357300 0.98947334 13.163695 110-119
ACTCA 2085500 0.98944783 12.059867 190-199
CGAGT 2600655 0.9884664 15.125929 160-169
ACCAA 2223710 0.9808014 16.907345 130-139
CCGTC 2835835 0.9734693 10.40882 140-149
ATGAC 2128635 0.9703505 10.671596 160-169
AAGGT 2200190 0.9636789 20.421541 15-19
ATGAT 1703875 0.9628042 8.877349 120-129
GCCAA 2606870 0.9586809 14.580454 180-189
AACTC 2017795 0.9573257 15.785696 190-199
CCAAC 2488250 0.952366 14.050854 130-139
GATAC 2087055 0.95139605 23.456713 35-39
GCAGT 2502490 0.9511555 6.4209332 50-59
GGGAC 3222700 0.9494475 10.097914 90-99
GGTAT 2014435 0.94908524 9.053832 110-119
TTTAG 1559325 0.94779974 29.326805 45-49
CCGAG 3079585 0.9442751 17.585917 220-229
TTTAT 1201480 0.9421597 10.908038 240-247
AGCCC 2951550 0.941915 11.570591 80-89
CGGAC 3068020 0.94072896 10.827972 220-229
CATAC 1964410 0.9319977 13.803127 130-139
CGTAT 1900620 0.93197125 7.4313054 240-247
ACAGG 2628990 0.92894137 14.938036 70-79
GCCAT 2347730 0.9287152 7.5157137 60-69
CAGAA 2191000 0.92851746 17.825949 140-149
TACAC 1955975 0.92799574 21.550602 35-39
CCCAG 2886715 0.92122453 14.848516 80-89
TCATA 1563000 0.9192094 8.915101 110-119
AATCA 1677825 0.9173231 13.8875 110-119
AACTG 2012025 0.9171931 11.445463 60-69
TATGG 1944740 0.916249 12.357956 35-39
CCCCA 2755180 0.9150964 16.983313 80-89
TGGTT 1800305 0.9123843 9.392276 50-59
TAACA 1667310 0.91157424 8.564472 120-129
GAGAT 2068175 0.90585655 11.892618 210-219
TCCTT 1635380 0.89776134 10.913341 1
AAGTT 1587060 0.8967958 17.304962 140-149
GACTG 2355875 0.8954294 12.791637 90-99
CGGAG 3016325 0.8886469 73.708 6
GTTAT 1448615 0.8805073 10.713887 110-119
CATTA 1484995 0.8733342 12.1329365 110-119
AGAAG 2135610 0.8695896 15.595756 140-149
TATTT 1104020 0.86573493 10.348618 240-247
ACGCT 2187350 0.865272 15.023492 190-199
ATAGT 1523815 0.8610581 15.59021 120-129
TCTTC 1566260 0.859817 48.209576 2
GTCTG 2087175 0.8533315 16.247118 3
TTACC 1668305 0.8514083 9.989166 45-49
AGTCT 1731900 0.8492391 15.933872 8
GATGA 1931865 0.8461531 6.470533 60-69
GGTAG 2309550 0.8434344 11.86398 120-129
GTTTC 1598745 0.843269 5.085891 100-109
GATCA 1841780 0.83958596 9.210945 110-119
GCCTA 2112780 0.8357737 16.302088 190-199
CTATC 1633035 0.8334085 8.266837 110-119
CATGG 2188890 0.8319612 8.57297 200-209
ATCCA 1751285 0.8308823 9.718562 180-189
GGTTC 2017905 0.8250108 11.18446 160-169
GTCTA 1681385 0.8244691 10.037044 30-34
ACAAA 1611825 0.8192451 12.545093 130-139
GCTTG 1996955 0.81644547 16.838425 90-99
GGTGC 2564225 0.81261903 11.5301695 60-69
ATATG 1437980 0.8125556 5.599707 50-59
CCTCC 2270775 0.81127983 10.719941 140-149
TCATC 1589345 0.81111157 10.43374 110-119
ACATC 1706415 0.8095941 5.8189907 110-119
TGGCA 2115750 0.8041619 5.654981 100-109
ATATA 1183135 0.80183095 10.543942 130-139
TTATG 1302320 0.79158515 11.147579 50-59
TATAC 1326355 0.7800371 8.894691 130-139
TGATG 1648665 0.77675563 11.790148 120-129
CCAGC 2382190 0.7602177 6.401513 80-89
CCCCC 2617885 0.75452626 13.522738 80-89
CGCCG 2827755 0.752411 11.084333 220-229
GTTCA 1533080 0.75174755 13.53399 160-169
CTGAC 1899165 0.75127184 9.389036 220-229
GGTTA 1581640 0.7451773 8.315333 50-59
ATCTT 1173220 0.7421892 13.660403 3
GGGAT 2029120 0.7410229 13.18603 100-109
GCGGA 2503245 0.7374872 9.1213455 220-229
TCAAC 1537120 0.72927356 7.4393744 110-119
GACCG 2374535 0.7280897 9.45528 210-219
CATCC 1767405 0.7276554 6.6086373 110-119
ATAAA 1152145 0.7258985 7.6458964 130-139
TTGGA 1536550 0.7239334 6.4538255 60-69
GATTA 1278925 0.7226788 5.5210576 50-59
TTTAC 1131745 0.71595186 13.5377035 45-49
ACGCC 2239470 0.71467215 12.85393 180-189
AACGC 1942950 0.714523 14.262004 180-189
TATTG 1168330 0.71014243 6.4474287 240-247
TCCGG 2151655 0.70967376 11.758852 70-79
ATGCC 1778025 0.7033512 8.018806 50-59
GGGCA 2381140 0.70151347 13.438477 150-159
GACCA 1899330 0.6984817 6.058023 170-179
CACAA 1580170 0.6969582 8.265968 130-139
GGATA 1587135 0.695162 15.33969 35-39
TGCCA 1751585 0.69289213 7.2113957 60-69
CAACG 1881575 0.69195235 14.510287 180-189
AATAC 1265230 0.6917436 11.145584 130-139
AAATA 1095285 0.6900744 12.278127 130-139
TTTCT 1013850 0.68990403 9.036045 4
TATAG 1214445 0.68624324 6.3513813 120-129
CAAAC 1546350 0.6820414 13.5240345 130-139
GAAAT 1293335 0.6794093 7.192312 110-119
GCCGC 2532340 0.6738067 12.181601 220-229
CCCGT 1935015 0.664241 10.152942 140-149
ATTAA 979225 0.66363764 6.251857 110-119
CAGTC 1675745 0.66289127 5.2907867 210-219
AATAA 1043835 0.6576588 7.213361 130-139
AGGCA 1860325 0.6573372 6.476541 80-89
CACGC 2040380 0.6511374 12.151678 190-199
GCGAC 2099045 0.64361787 7.125116 70-79
ACTTC 1254185 0.6400649 30.088165 4
AGATG 1454180 0.63692796 5.592874 120-129
GACGA 1796930 0.63493675 9.601865 220-229
TCCAC 1530400 0.63007843 10.27485 180-189
CCGTA 1591440 0.6295419 6.27683 230-239
TCAAT 1061975 0.6245537 8.359428 110-119
TACTT 982900 0.6217911 11.461163 1
CACCG 1935230 0.61758137 8.911509 45-49
CCGGC 2320285 0.61738294 9.54104 70-79
AAGCA 1449745 0.61438316 5.9115896 120-129
CCCTA 1472955 0.60642785 11.740983 110-119
CACGA 1645925 0.60529166 8.785412 180-189
GAAGA 1479390 0.6023863 6.348222 15-19
GGCCC 2261055 0.601623 10.286513 70-79
AGTAA 1144510 0.60122925 5.164191 50-59
GGTGA 1639225 0.59863555 6.44681 120-129
TAGCT 1216500 0.59651214 12.165227 50-59
AATGG 1355585 0.5937436 6.565274 120-129
CTTTG 1111965 0.5865136 7.1433306 45-49
TGATT 964190 0.5860607 5.9535584 50-59
ACCCG 1826935 0.58302164 8.807665 140-149
ACCTA 1227155 0.5822133 7.1757154 130-139
GGGGA 2051380 0.5806868 9.381659 4
ACGAC 1569900 0.57733333 8.966182 220-229
TAATA 839030 0.56862503 7.86659 130-139
GTTCC 1336025 0.5684977 6.6514664 140-149
GTCCT 1328545 0.5653149 6.232653 45-49
TTCAA 947140 0.5570186 5.4099774 200-209
ATTTC 878250 0.55558866 6.076387 240-247
CGGTT 1350080 0.55197376 11.070657 160-169
TTAGC 1123560 0.5509389 9.388675 45-49
CTGCG 1667025 0.5498298 11.942526 25-29
CGGCA 1792345 0.5495762 10.918344 70-79
AAGAT 1040345 0.5465097 5.7698674 15-19
TCAGG 1435790 0.5457203 5.3195214 150-159
GGAGG 1903430 0.53880644 5.4935656 6
CCTAT 1052110 0.53693724 5.149213 110-119
CCACG 1676980 0.5351672 7.7373753 180-189
AAATC 970800 0.5307689 7.7617674 110-119
ACCCC 1593055 0.529112 9.101998 140-149
GCTAC 1336815 0.5288174 7.8882184 50-59
CTGTT 1001260 0.5281215 5.6365438 240-247
ACATT 882940 0.51926214 6.201736 110-119
TATGT 851575 0.51761025 6.422452 140-149
GGGTA 1370350 0.5004439 5.2645082 100-109
GGGTT 1267010 0.49771833 5.844756 100-109
CGCGG 1920720 0.49104595 6.561727 220-229
AGTAT 868660 0.49085143 5.206815 110-119
GTAAC 1071485 0.48844263 6.769614 120-129
TGACG 1269380 0.48247054 8.501878 220-229
TATCC 939945 0.47969463 5.4951534 110-119
AACAT 869020 0.47512233 5.6774106 110-119
TGGCC 1422010 0.46901718 14.868001 2
ATGCT 941035 0.46143755 7.08151 50-59
CCGGT 1394425 0.45991892 8.614611 160-169
TAAAT 678460 0.459804 7.6574335 130-139
CAATC 965500 0.45807326 6.277111 110-119
CATAA 834615 0.45631203 5.761809 110-119
CCGCG 1712195 0.455582 6.7955766 220-229
GGTTT 898020 0.4551114 5.3668857 100-109
TGCTA 921205 0.45171392 6.3835635 50-59
ATTCT 706425 0.44689065 7.4228067 2
TTGGT 869740 0.4407792 5.2512045 120-129
TGCGC 1331160 0.4390524 8.913365 25-29
GCCTT 996260 0.4239229 8.956777 1
GGTCA 1111810 0.42258078 5.359914 160-169
GCGCT 1269385 0.41867736 8.076477 25-29
GCATG 1088775 0.41382554 8.052763 60-69
CACTT 805625 0.4111453 6.988578 3
ACGAG 1153005 0.40740898 7.723361 180-189
CTAAC 845765 0.40126604 5.324134 110-119
AGCTT 810560 0.39745897 7.5243206 1
CCGGG 1553910 0.3972683 5.2760344 80-89
ATGGC 1042415 0.39620483 7.1656284 1
CCTAA 821465 0.38973707 7.409668 110-119
CATGC 980385 0.38782072 6.8266125 60-69
GGTAA 871985 0.38192773 7.1131644 120-129
GAGGC 1280410 0.3772247 5.8412538 35-39
TTTGA 618130 0.37571606 5.8327208 45-49
TTCTT 550040 0.37429088 20.262833 1
TGGGC 1112285 0.35249013 9.476176 1
CTCGG 1059640 0.3494978 5.394692 6
TGCTG 844755 0.34537405 13.519592 1
CGAGC 1117805 0.342746 6.5543456 180-189
AACCT 676085 0.32076278 5.2956133 2
CTCTT 578545 0.31759918 9.414393 1
GCTTT 594550 0.31359947 5.0483284 45-49
GCGAG 1042555 0.30714968 5.380424 240-247
TCACA 647205 0.30706093 5.492145 5
CGGGA 1029820 0.30339783 5.020892 80-89
AACTT 515760 0.30332145 10.73454 3
CGCTT 708420 0.30144286 5.74151 1
GACTT 603840 0.29609358 6.884262 1
ATTGT 474120 0.28818294 5.250582 240-247
TTCCT 519905 0.285408 6.465132 4
TCGGT 672950 0.2751324 6.3725147 7
GAGGT 752020 0.27463335 6.9055414 7
TAACT 460890 0.27105206 7.1650267 2
TGTAA 471485 0.26642078 7.9159236 25-29
GTCTT 503155 0.26539254 11.935175 1
CCCTT 597385 0.26455972 5.630199 3
TTGGC 631655 0.2582491 7.21311 1
AATCT 434920 0.25577897 7.1040106 2
CGGGG 1028480 0.252638 9.310731 3
CCTCG 701245 0.2407194 5.9695296 5
TGCTT 424045 0.22366545 11.80869 1
TGCCT 469805 0.19990875 5.0901237 4
TCGCA 459895 0.1819253 6.8810797 7
TAGGC 403190 0.1532459 6.6488314 1
TTGCT 245330 0.12940098 5.742801 4
Produced by FastQC (version 0.10.1)