FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_12_SL248341.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_12_SL248341.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9543351
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 10921355 8.448075 60.12624 110-119
CACAG 14538010 7.347758 41.66384 110-119
ATACA 9953930 7.3060474 36.98636 110-119
TCCCT 17057720 7.0799246 107.55923 30-34
CAGTA 12336215 6.5709043 38.826004 110-119
AGTAA 9462240 6.202428 46.603657 110-119
GGAGA 15068870 6.0742164 92.18155 35-39
CCCTG 17105895 6.016446 70.41397 30-34
GTAAT 10589195 5.9442835 39.246426 110-119
GGTGA 16797680 5.798661 101.25879 45-49
AATAC 7253615 5.324053 38.910606 110-119
GTAGT 12685835 5.167863 26.521404 80-89
AGACG 11340605 5.1187744 99.568665 40-44
ACAGT 9543755 5.083496 35.342045 110-119
GTGAC 12855135 4.9690714 83.52849 45-49
ACTCC 9780730 4.740347 122.101654 30-34
GAGAC 10452440 4.717886 139.00279 40-44
CCCCA 10240880 4.709587 100.87816 60-69
CTCAG 10608315 4.591612 34.64447 140-149
ACCAG 9078375 4.5883656 93.41617 45-49
GGCCC 14707210 4.3833995 59.656918 60-69
GCACA 8631170 4.3623405 34.133602 110-119
TAGTA 7758630 4.355335 33.397392 80-89
CACTC 8978115 4.3513503 98.75721 25-29
GTGTC 13052350 4.32072 23.856558 120-129
CGTGT 12955310 4.288597 23.264988 120-129
TGACC 9799035 4.2413297 142.55334 45-49
CTCCC 10689275 4.2098107 70.2048 30-34
AGTAG 8598900 4.090402 27.467478 70-79
ATGCA 7507175 3.9987087 95.23212 25-29
GACCA 7896215 3.9908817 105.45292 45-49
CCCAG 9617525 3.9499242 86.12206 60-69
TGAGG 10951460 3.7805107 68.37058 35-39
CCACT 7669235 3.7169862 27.49142 240-247
CAGGG 11281300 3.6952536 36.12593 50-59
AGGAG 9093045 3.6653798 92.60091 35-39
TACAC 6131705 3.6571655 27.303143 110-119
ATGGT 8938525 3.6413112 25.449762 190-199
GACGG 11115415 3.640917 106.53081 40-44
ACGGT 9377145 3.6246765 94.47706 40-44
CTGAG 9294115 3.5925817 104.512344 35-39
TGTCC 9602245 3.559265 20.652592 120-129
TGCAC 8214495 3.555491 115.98029 25-29
GGGTT 11862970 3.5070398 43.065456 50-59
AGATA 5349985 3.5068755 18.824606 150-159
CCCTT 8383920 3.4798045 33.792118 20-24
GAGGA 8559935 3.4504843 121.91429 35-39
GCCCC 10274110 3.4288232 73.26418 60-69
TGGTG 11446175 3.3838232 17.904459 190-199
CCTGA 7801360 3.376674 84.3226 30-34
AAAAA 3169245 3.342715 19.990213 130-139
CCAGG 9065030 3.3248687 41.015667 50-59
TCTTG 8461765 3.3055358 18.016088 170-179
GGATG 9564910 3.301865 69.72109 20-24
GCACT 7624720 3.300218 89.541275 25-29
CTTCA 6439990 3.2894056 16.580954 200-209
CCGTG 10383080 3.2613716 23.618465 120-129
CTCTC 7809965 3.2415805 13.505836 100-109
CTTGG 9753535 3.2287133 18.906767 20-24
AGGCT 8286675 3.2031622 23.377974 30-34
CCTGG 10193270 3.2017517 29.852608 50-59
TCAGG 8253605 3.1903791 20.679277 140-149
CAGAT 5944420 3.1663048 17.441814 150-159
TCGCA 7228045 3.1285248 24.14728 100-109
TGTCT 7974275 3.1151009 19.72946 180-189
TTCCC 7391950 3.0680804 50.804832 50-59
GGTTG 10373955 3.0668435 74.79471 15-19
CGCAC 7441640 3.0562863 24.769274 100-109
TCTCA 5964460 3.0465152 21.317478 130-139
CGGAT 7860500 3.0384269 79.31046 20-24
ACAGA 4795230 2.9825232 14.418322 140-149
GGTTC 8991405 2.9764252 48.675842 50-59
GATGC 7588905 2.9334435 96.971146 25-29
TGGCC 9326550 2.9295108 43.858982 60-69
AGGGT 8469850 2.9238439 50.48688 50-59
ACCAC 5099015 2.885734 19.150684 230-239
GTTGG 9651155 2.8531628 53.39022 15-19
GACTC 6547280 2.8338683 19.607748 190-199
TCTCT 6405810 2.802045 14.920589 180-189
GCCCT 7957760 2.7988849 18.346159 20-24
AGTCA 5229005 2.785238 32.04311 70-79
TCAAA 3781535 2.7755945 57.289707 70-79
CCAGT 6404105 2.7718978 37.15255 60-69
CGGTG 9834930 2.7588317 67.22437 40-44
GCCGT 8774815 2.7562084 22.32628 120-129
TTCTT 5886035 2.7134213 20.44692 170-179
CGGCC 9070605 2.7034416 12.872888 120-129
GTTCC 7273505 2.6960707 55.134396 50-59
GATGG 7763615 2.6800475 18.61402 15-19
TCACA 4394035 2.620758 11.808103 140-149
AGATG 5432720 2.5842853 16.646149 180-189
AGGGA 6268135 2.5266666 9.122051 150-159
GCGGA 7707760 2.5247204 87.4971 20-24
TCCAT 4844995 2.4747171 16.383509 70-79
GGCTG 8811560 2.4717624 12.16888 30-34
GGCGG 10347465 2.4596562 42.719826 20-24
TCCTC 5921095 2.4575918 16.890411 130-139
TGTTC 6272020 2.4501255 12.865799 140-149
TGGAG 7031775 2.4274116 15.832538 25-29
ACTCT 4699770 2.4005392 13.620213 200-209
ACAGC 4727090 2.3891518 11.037421 120-129
TTGGG 8074015 2.3869143 80.27116 15-19
CTGGC 7553460 2.3725753 35.559235 60-69
TACCA 3957640 2.3604763 15.943772 230-239
GAGAT 4949015 2.354192 16.24966 180-189
GTTCT 5990870 2.340296 17.5078 160-169
CAGCT 5368690 2.3237376 12.314763 150-159
AATAA 2570100 2.3214662 9.628156 110-119
CACGG 6284970 2.3051991 9.722608 120-129
CTGGA 5902955 2.28175 14.292944 180-189
TGTAG 5558045 2.264196 8.075342 210-219
TCAGC 5143570 2.2262986 14.443062 140-149
CATTT 4132050 2.2242882 17.430647 150-159
TCATT 4124355 2.2201462 16.25966 150-159
CTACT 4337105 2.2152982 7.578142 230-239
ACTAC 3705175 2.2098973 12.343352 230-239
TTCAT 4076795 2.1945446 15.947281 140-149
TTCAC 4292420 2.1924736 18.227898 200-209
CAGAG 4855385 2.1915603 9.576604 140-149
CACTA 3620790 2.159567 15.002864 240-247
GTGAA 4528605 2.1542075 17.465525 190-199
TCTGC 5768245 2.1381161 11.931042 210-219
TTGTC 5455540 2.1311727 13.178632 170-179
TGGGG 8506250 2.1309435 52.96855 15-19
GATAC 3991435 2.1260443 14.296463 150-159
CTCGC 5952320 2.0935361 16.5921 100-109
ATTTG 4334490 2.0837395 15.346393 150-159
TAGTC 4552555 2.076669 26.556656 70-79
TCTCG 5597330 2.0747628 16.912428 100-109
GAACT 3881740 2.067615 11.012603 160-169
TTGGA 5052890 2.0584095 15.677755 170-179
ATCTC 4024085 2.0554142 8.8912115 100-109
GCTGT 6179390 2.0455637 10.092685 140-149
CTCTG 5483325 2.0325046 14.3024435 180-189
GTCTC 5472990 2.0286736 11.690602 180-189
GTCCT 5449565 2.0199907 14.314917 130-139
ACACA 2898945 2.0189915 13.327531 110-119
AGCTC 4652065 2.0135598 12.4157 140-149
GATAT 3586070 2.0130537 8.176106 180-189
ATATA 2597715 2.0094292 10.150505 110-119
GAATC 3763225 2.004488 14.098167 190-199
GTCAA 3756525 2.0009189 40.6123 70-79
GTTCA 4384430 1.999978 14.902058 140-149
ATGTA 3548840 1.9921544 7.12429 70-79
TACAG 3728450 1.9859648 13.426279 160-169
ACACG 3928335 1.9854474 13.466577 120-129
CAGGC 5395810 1.9790734 15.152779 140-149
TGAAT 3485180 1.9564186 13.481232 190-199
AACCA 2789940 1.9430742 14.942764 70-79
ACGGC 5292435 1.9411576 14.503385 120-129
AATAT 2507120 1.9393507 12.786639 110-119
TTGCA 4250220 1.9387574 14.076033 150-159
CCTTC 4656605 1.9327567 13.767064 200-209
AATCG 3620645 1.9285425 12.953274 190-199
TACTA 3045050 1.9140404 9.855909 230-239
TTTGC 4875055 1.9044101 12.098448 150-159
GGGGC 7984055 1.8978591 48.381012 15-19
ATATG 3372840 1.8933561 8.449008 180-189
GAGTC 4896440 1.892688 12.002466 210-219
TGGAA 3964595 1.8859142 8.964922 170-179
CAGCC 4562550 1.8738422 10.146886 120-129
TCGGC 5947640 1.8681802 7.833424 130-139
GGGCG 7756765 1.843831 60.201035 20-24
CATAG 3459245 1.8425723 8.128956 210-219
GTTGT 5147520 1.7958035 7.075551 210-219
AGTAT 3168450 1.7786212 11.557307 220-229
CTGTT 4540300 1.7736402 12.647953 140-149
GACGT 4557845 1.7618064 9.726166 170-179
GTCAC 4060925 1.7576956 8.666079 130-139
CTCCA 3625460 1.7571222 7.7617364 150-159
CGTAG 4524240 1.7488164 9.590555 210-219
AGAAC 2811395 1.7486233 11.544085 160-169
CCAGA 3441715 1.7395015 25.362745 60-69
CCTTG 4674100 1.7325494 21.642412 20-24
CACCA 3040510 1.7207447 13.18037 230-239
TTGTA 3574995 1.7186239 9.257003 210-219
GCAGA 3762780 1.6983945 14.208394 150-159
TGCAG 4381265 1.6935503 13.436231 150-159
ATTGT 3511595 1.6881455 7.4150677 50-59
AGTCT 3692950 1.6845564 12.837718 210-219
GGTCA 4342050 1.678392 7.7272863 130-139
TTCAG 3659080 1.6691061 9.562482 150-159
TGGTT 4767235 1.6631345 18.024496 10-14
TCCCA 3426575 1.6607302 10.700542 240-247
TGACT 3639725 1.6602774 11.734763 190-199
GTCTG 4980505 1.6486968 8.922653 210-219
AACTG 3084720 1.6430812 10.848424 160-169
ATAGT 2926470 1.6427847 7.226237 220-229
CAAAA 1903915 1.6317986 20.409483 70-79
GAGAA 2920045 1.6219736 10.496254 160-169
TCAGA 3024295 1.6108956 9.839454 130-139
GCTCC 4547035 1.5992726 6.2823668 150-159
TGCTC 4299960 1.5938665 5.203141 150-159
ACTGA 2989240 1.5922234 8.870151 180-189
TGTGC 4797830 1.588226 8.036803 220-229
GTGCT 4795085 1.5873172 8.125382 220-229
CTGTG 4785245 1.58406 5.208424 210-219
CTGGT 4781630 1.5828632 7.159363 160-169
CAAAG 2542370 1.5812957 29.010996 70-79
TCTGG 4776100 1.5810326 11.769199 180-189
AATAG 2402925 1.5750993 9.338564 240-247
TAGTT 3274995 1.5744036 9.815995 220-229
ATAGA 2397395 1.5714744 10.055085 240-247
TTGGT 4478825 1.5625175 23.181147 25-29
GTCCC 4413535 1.5523183 19.432013 50-59
GAACC 3069070 1.5511605 12.474962 70-79
GGCCG 5815175 1.5478305 13.195704 120-129
GGACG 4714060 1.5441171 7.29087 170-179
GCTCA 3561180 1.5413903 11.199688 140-149
CCACC 3296760 1.5161176 9.656699 230-239
CCCAC 3294615 1.5151311 10.35703 240-247
ACCAT 2498155 1.489988 11.130215 45-49
GCTCT 3941890 1.4611406 9.866157 130-139
CCTCG 4151165 1.4600382 9.618556 130-139
AAATA 1605905 1.4505483 5.292853 9
CGACT 3336330 1.4440684 8.721883 190-199
CACTG 3298700 1.427781 7.217939 230-239
TCGAC 3264780 1.4130993 9.480712 190-199
TGATA 2509495 1.4087143 7.6524143 180-189
CAGAC 2777965 1.4040308 14.609287 60-69
TATAC 2233540 1.4039462 6.5765443 110-119
CTCTT 3209075 1.4037215 8.308295 200-209
TGGAC 3609395 1.3951888 10.376917 170-179
GAGGG 4765690 1.3940898 8.268573 200-209
CATGT 3050745 1.3916115 7.194667 70-79
CTGCT 3749490 1.3898239 5.8288803 90-99
GCTGA 3594575 1.3894603 19.738731 30-34
GGGAG 4720115 1.3807579 6.6200986 150-159
CTTGA 2999980 1.3684548 8.505698 200-209
GCGTA 3524720 1.3624582 10.061186 210-219
AATTG 2423165 1.3602526 8.98812 170-179
AGATC 2524560 1.344711 6.846173 130-139
CCTCA 2754005 1.3347613 7.23488 130-139
CCATC 2728870 1.3225794 10.465839 240-247
CTCGA 3052745 1.3213238 8.484795 190-199
CTGAT 2887410 1.3171055 7.4347153 180-189
GTAGG 3805655 1.3137352 5.071045 160-169
ACTTC 2566780 1.3110548 6.5275 220-229
ACGGA 2902940 1.3102912 9.091611 200-209
TCCAC 2692785 1.3050903 9.586204 240-247
TATGG 3170010 1.2913755 6.093642 180-189
ATCGG 3313090 1.2806541 7.718365 190-199
GTGGA 3695950 1.2758645 20.079504 25-29
GAATT 2261240 1.2693555 9.960482 170-179
ACGTG 3282670 1.2688954 9.278041 170-179
GTGTT 3622065 1.2636216 7.498705 160-169
CCATG 2915915 1.2620996 7.323384 70-79
GGAAT 2649150 1.2601715 8.341494 170-179
ACTAT 2004645 1.2600685 6.194912 230-239
AGCCG 3417200 1.2533594 8.994957 120-129
ACTCG 2869585 1.2420464 8.690509 190-199
GGAGT 3593965 1.2406586 6.8757524 210-219
GGAGG 4234000 1.2385565 15.461716 30-34
ATAAA 1366680 1.2344662 5.2310686 7
ACCGT 2848765 1.233035 17.232475 45-49
AAAGT 1880045 1.2323555 21.14535 70-79
GGCTC 3922505 1.2320763 8.25737 130-139
AAGTA 1858380 1.2181542 15.5380535 70-79
ATAAC 1659535 1.2180756 7.5377502 80-89
TTGAG 2966540 1.2084874 8.169847 200-209
ACTGG 3125140 1.2080032 7.31189 160-169
GATCT 2645110 1.2065792 5.444211 130-139
GTCCA 2768800 1.1984234 15.400324 70-79
GTGGT 4025985 1.1901985 10.026596 50-59
AGAGC 2624430 1.1845809 7.2124405 140-149
TTGGC 3565495 1.180286 7.8669105 60-69
AGCTT 2582345 1.1779487 6.3558354 150-159
GCATA 2198350 1.1709546 7.830215 210-219
TGGTC 3520005 1.1652274 11.421863 50-59
TTCCA 2273845 1.1614301 7.3280454 230-239
TGCCC 3268510 1.1495928 6.453785 60-69
ACTGT 2504165 1.1422864 5.5705857 230-239
TATCA 1813720 1.1400579 6.6940603 70-79
AAAAG 1484545 1.1362981 13.5677595 70-79
ATACC 1899470 1.1329112 6.921034 70-79
TCACG 2616955 1.1327003 9.3680725 200-209
ATATC 1787955 1.1238629 7.1971846 70-79
GAAGA 2013150 1.1182281 17.903126 35-39
TCTGT 2859200 1.1169288 6.3381786 210-219
TCTAC 2185780 1.1164484 7.4394097 180-189
CAATA 1509765 1.1081467 6.4406614 100-109
GTCTA 2417740 1.1028632 7.4684544 180-189
GCTTC 2953470 1.0947629 5.70461 150-159
AGGTT 2685180 1.0938691 22.73685 10-14
AGTTC 2393670 1.0918838 5.2637177 160-169
TGTAA 1944160 1.0913614 6.7966366 230-239
ATCCA 1816215 1.0832547 15.040797 240-247
CCATA 1811785 1.0806127 9.648808 70-79
TACTG 2366620 1.0795445 6.5940337 180-189
CTGAA 2026350 1.0793387 14.102663 35-39
CGGCT 3430070 1.0774002 7.772644 130-139
CTCGG 3411925 1.0717008 8.348209 130-139
GAGCT 2767555 1.069781 6.4507523 140-149
TATGT 2215715 1.065171 7.362442 220-229
CTGCG 3351765 1.0528044 6.7974615 210-219
GGGGT 4182585 1.0478004 7.938482 10-14
CTTCT 2394015 1.0471959 5.4326134 210-219
CTGCC 2965365 1.0429714 5.79449 200-209
CTACC 2149485 1.0417736 8.489555 230-239
GTCCG 3314515 1.041104 5.144216 130-139
AGAGA 1871800 1.0397135 12.416027 35-39
TAGAT 1845525 1.0359923 7.8915067 240-247
AAAAT 1146230 1.0353427 5.231707 8
GCGGT 3680190 1.0323433 5.873388 130-139
AACTT 1627835 1.0232155 5.7910123 200-209
CATGG 2609810 1.0088056 5.2893095 190-199
ATGGG 2911890 1.0052022 29.14232 15-19
ATACG 1869180 0.99562174 5.098397 120-129
TGCGT 2995650 0.99165016 8.043403 210-219
TATAA 1280090 0.9901973 6.2975554 240-247
AAGAG 1758020 0.97651297 13.2839775 35-39
CAGCG 2649520 0.9717901 6.2320905 160-169
TCCCC 2453420 0.96624273 5.0870924 80-89
CGAAC 1902095 0.9613512 13.1465 70-79
ACCCA 1691035 0.9570235 12.547439 240-247
ACAAT 1293390 0.94933033 7.4930162 100-109
AGCTG 2449040 0.946661 5.084927 90-99
CGGAG 2884070 0.94469345 6.456566 210-219
TTCTG 2375205 0.927859 5.3333893 210-219
ACTAA 1259085 0.9241509 12.031795 240-247
TAGGC 2385780 0.9222082 5.701489 160-169
CCCAA 1621245 0.91752666 9.645876 240-247
CCATT 1793285 0.9159707 5.7195716 50-59
CGTGG 3227075 0.9052384 6.993538 50-59
TGAAC 1698970 0.9049591 6.4286885 190-199
TGCAT 1970035 0.8986405 6.0467057 210-219
TCGAA 1677585 0.8935684 16.701262 70-79
ACGTC 2029465 0.87841606 10.576415 70-79
GTATG 2155955 0.87827724 5.1728296 220-229
CATCA 1457335 0.869206 5.571176 230-239
TGAAG 1826970 0.8690695 9.205405 35-39
CCAGC 2098870 0.86200726 5.801078 80-89
CCAAT 1442350 0.8602686 10.539185 240-247
GGTGG 3416465 0.8558758 20.431328 25-29
CGGTC 2711465 0.8516832 5.6917005 130-139
AGCGT 2185995 0.8449826 6.412201 160-169
ATCAA 1147910 0.84255 12.502311 70-79
CTTCC 2007385 0.8331791 5.2079086 230-239
AAAGA 1086960 0.8319792 5.6613407 8
GAGGC 2534160 0.8300785 12.983862 30-34
GGGCC 2959905 0.7878406 15.382539 15-19
TAACC 1320410 0.78753924 6.1581526 80-89
AACCG 1542905 0.7798105 5.6428423 190-199
GACCG 2107310 0.77291846 16.024397 45-49
TGAGA 1623595 0.77232623 5.3958435 240-247
CGTCC 2155365 0.75808 8.741557 70-79
AAAGG 1353705 0.7519315 8.645656 10-14
AGCCA 1477115 0.7465591 5.9960833 80-89
CTAAT 1178905 0.74102956 9.258304 240-247
ATGAT 1318025 0.7398782 6.8185196 240-247
AATCC 1230180 0.7337229 9.411295 240-247
TGGGC 2610150 0.73218256 16.714312 15-19
GGTCC 2321075 0.72906 11.7032995 50-59
GTCGA 1876885 0.7254981 12.082299 70-79
GTAGC 1809735 0.6995416 5.509887 80-89
AAGGT 1460290 0.69464386 9.685126 10-14
GGAAG 1719275 0.6930347 5.909002 30-34
CATAC 1153305 0.6878719 8.274571 70-79
AAAGC 1085715 0.67528987 9.398113 70-79
TATGA 1170760 0.6572104 5.6175404 240-247
CATTG 1439975 0.65685123 5.17929 50-59
GAAAA 856430 0.6555273 5.9621944 8
TAGGT 1606070 0.6542691 5.2808948 10-14
CAATC 1085170 0.6472338 9.125962 240-247
AGGAT 1348000 0.6412288 5.4130197 10-14
GAGGT 1850015 0.6386364 6.7383547 10-14
AGAAG 1129170 0.62721086 5.3083515 9
ATAGG 1288440 0.61289674 5.148855 10-14
GGGTC 2150815 0.6033328 5.8721795 70-79
TAAAA 666750 0.602248 6.0668154 8
AAGAA 777555 0.5951549 5.134658 9
CGATG 1524805 0.58940375 12.976541 15-19
AATGG 1222940 0.58173907 6.475788 10-14
CATCC 1169530 0.5668266 7.173323 240-247
GAAGG 1355305 0.5463195 5.456191 10-14
AAGGG 1294670 0.52187765 6.28508 10-14
AAGCA 832375 0.5177182 6.1684313 70-79
GGTCG 1786760 0.50121045 5.9415264 70-79
TGATC 1089210 0.4968482 5.2455235 240-247
GATCC 1105335 0.47842368 5.345881 240-247
ATAAG 719505 0.47163013 5.327536 9
CGGTT 1360650 0.45041603 8.092241 10-14
ACGAT 844665 0.44991216 5.72147 10-14
TAAGG 927450 0.44117776 5.6365256 10-14
TAAAG 620895 0.40699202 5.474084 9
TGGGT 1338175 0.39560354 5.0178285 10-14
AAGCT 740970 0.39467883 7.40513 30-34
Produced by FastQC (version 0.10.1)