FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_8_SL248354.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_8_SL248354.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11047110
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 47541 0.4303478466313814 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 38064 0.3445607041117541 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 37875 0.34284984941763047 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 36816 0.33326363184579494 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33743 0.3054464018191183 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 32546 0.29461098875633535 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 32532 0.29448425877899287 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 32127 0.2908181415772994 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 31725 0.28717918079932214 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30652 0.27746623325014413 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 29467 0.26673944588222626 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 29292 0.2651553211654451 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 28259 0.255804459265817 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 28162 0.2549264015656584 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 28124 0.2545824201985859 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27920 0.25273578338588104 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27796 0.2516133178722761 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27720 0.2509253551381312 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26876 0.2432853479326267 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26309 0.23815278385025587 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26156 0.23676780624072724 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26131 0.23654150270975846 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25739 0.23299306334416875 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24853 0.22497286620663687 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24173 0.21881741016428732 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24146 0.2185730023508411 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23241 0.21038081452977295 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22432 0.20305763226762474 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22349 0.20230630454480858 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22020 0.19932815007726004 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21994 0.1990927944050525 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21942 0.19862208306063756 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 21493 0.19455767164443913 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20852 0.1887552491104008 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20736 0.1877052007267059 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20574 0.1862387538460285 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 20136 0.1822739159834563 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20065 0.1816312139555051 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 19910 0.18022813206349897 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19835 0.17954922147059277 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19483 0.176362867754553 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19069 0.17261528128171078 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 18715 0.16941082328319354 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18656 0.16887674695010732 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18341 0.1660253224599013 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17925 0.16225963170458158 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17887 0.16191565033750907 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 17549 0.15885602659881182 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17439 0.1578602910625494 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 17438 0.15785123892131064 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16641 0.1506366823540274 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16627 0.15050995237668496 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 16258 0.14716971225958644 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16036 0.1451601369045841 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15584 0.14106856906466939 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 15146 0.1371037312020972 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14715 0.13320225832819624 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14612 0.13226988778060506 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14593 0.13209789709706882 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14527 0.13150045577531136 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 14253 0.12902016907589406 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13243 0.11987750642475724 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12941 0.11714375977065494 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12639 0.11441001311655265 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 12068 0.10924124046922679 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11845 0.1072226129729857 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11479 0.10390952927960345 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 11160 0.10102189622444241 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11103 0.10050592417383371 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 22459660 7.6661143 67.95099 35-39
TATTA 11075960 7.311507 127.480545 240-247
TGGAT 17016240 7.1875362 80.5999 35-39
TCACC 17375225 6.460512 75.369286 160-169
TCTCC 15566860 6.1784782 88.67332 20-24
CTGTG 16292375 5.9360914 88.73151 25-29
TCCCT 14567135 5.781688 72.3173 15-19
CTGAG 16591890 5.6632795 51.15158 15-19
AGTGG 17306645 5.659823 39.846615 100-109
TGTAT 10151215 5.538102 59.681114 240-247
TTACT 9257315 5.2712083 111.32507 240-247
ATTAC 9312150 4.967423 105.12308 240-247
TGGGT 14163335 4.944239 45.93811 100-109
CCTGA 13877405 4.9438195 52.88373 15-19
TTCAC 10375140 4.7739162 76.158195 40-44
CTCTG 12546225 4.771022 74.16036 35-39
CTCCA 12588245 4.680602 42.330585 80-89
GACAC 13855955 4.6243076 47.430023 230-239
ACACG 13838655 4.618534 49.93899 230-239
TGAGA 11468760 4.5382547 96.63729 15-19
ATTCA 8390160 4.4756017 114.556946 40-44
TCTGG 11871505 4.3253565 136.13757 35-39
AAGGG 14117990 4.325325 39.58001 90-99
TACTG 9679215 4.267159 78.37739 240-247
AGCCT 11873810 4.230039 52.68109 30-34
CAGCC 14552965 4.1894927 56.895096 30-34
GAAGG 13634970 4.1773424 31.665916 150-159
CCAGG 15111745 4.1681404 53.714905 80-89
CCCTG 13555705 4.165586 85.96829 10-14
ACCAT 9576730 4.128136 67.92621 160-169
GAGAC 12854315 4.110333 85.5854 8
TGGAG 12552700 4.1051316 38.763504 90-99
TCCAG 11416985 4.0672956 41.703022 80-89
TCAGT 9037640 3.984315 96.47425 7
GGGTC 14121840 3.9836476 129.29242 8
CACCA 11365725 3.9590375 57.973152 160-169
ACTAC 9119520 3.9310517 34.70117 130-139
GTATT 7202575 3.929441 68.79016 240-247
TACTA 7291300 3.8894317 29.295622 130-139
GACTC 10810445 3.8512158 53.204742 20-24
CTGGG 13649545 3.8504171 136.47061 3
GATTC 8676005 3.8248851 60.021698 40-44
AGACT 9199105 3.7992704 71.30649 15-19
CACCT 10154590 3.7757125 44.038265 40-44
ACTGT 8518020 3.7552361 67.39091 240-247
TGAAG 9461280 3.7438834 40.46904 150-159
GGACA 11438265 3.6575332 28.543123 220-229
CAAGA 9383310 3.6305017 44.583138 180-189
GCTGG 12627910 3.562223 34.430344 2
ACTCT 7728040 3.5559053 70.098946 20-24
ACTGG 10394485 3.5479305 44.365067 60-69
CCTGT 9270060 3.5251765 62.798134 25-29
TCCTG 8937640 3.3987653 64.837654 20-24
TATCT 5899130 3.3590245 49.7095 200-209
GAGTG 10261895 3.3559656 38.82885 90-99
CTCCT 8374385 3.323789 95.4941 20-24
AGACA 8513235 3.2938607 37.798126 170-179
GGATT 7784895 3.288283 57.36185 40-44
GGTCC 11129025 3.2766414 145.46204 9
CTGCA 9089760 3.238223 45.122616 25-29
CATCT 6999020 3.2204611 33.566494 170-179
AGCTG 9281680 3.1680987 35.98731 60-69
GTCTC 8240190 3.1335423 33.426933 30-34
CCATC 8391665 3.1202161 34.737263 45-49
GTCCC 10044010 3.0864635 76.80546 10-14
GGAGT 9173815 3.000129 37.12634 90-99
CCTCA 8040445 2.9896243 81.87514 5
GGCTG 10539740 2.9731684 34.795147 90-99
AAGAA 6567840 2.9460049 37.53936 180-189
AGTAG 7263055 2.8740327 28.828785 50-59
AGGGA 9346150 2.8633776 40.675 80-89
TGTGC 7734345 2.8179915 58.56909 25-29
GTGAA 7114050 2.8150709 39.475143 150-159
ATCTC 6084825 2.7998126 49.07277 170-179
ATCTG 6325225 2.7885256 37.170036 200-209
GACAA 7203435 2.787085 25.25699 180-189
GAGCT 8150720 2.7820704 29.288973 60-69
GAACA 7123255 2.7560627 35.709656 210-219
CAGGG 10395650 2.7472441 36.64599 80-89
GGAAG 8956445 2.7439837 30.488476 80-89
ATGAA 5646120 2.7033672 28.058235 210-219
ACCTT 5851145 2.692289 71.50158 40-44
GTGTA 6343140 2.6792965 35.426117 230-239
CTATG 6004065 2.6469395 27.025463 50-59
CGCCA 9073560 2.6120875 40.63616 70-79
GGGAA 8491290 2.6014743 34.162052 80-89
CACGG 9362210 2.5822966 44.629116 230-239
TGCAA 6233375 2.5744107 36.27183 200-209
GAGTC 7472450 2.5505576 27.113222 150-159
AGAAC 6590435 2.5499089 30.647982 190-199
AGGGC 9556735 2.5255451 33.188293 150-159
AGGCT 7321900 2.4991708 20.386578 80-89
CAGTG 7290085 2.4883113 74.59501 8
CCAAG 7445785 2.4849677 38.996128 180-189
CCCTC 7700575 2.4697862 26.625004 20-24
ACGGC 8918660 2.4599562 49.04947 230-239
CTACA 5664290 2.4416435 19.486225 190-199
CACTG 6839685 2.4366345 19.532711 60-69
ATCAG 5870295 2.4244576 26.729353 45-49
TCTGT 5137985 2.417884 23.939793 210-219
TATAT 3655110 2.4128258 24.115139 240-247
CTCTC 6045375 2.399406 100.20229 20-24
TGAAC 5785450 2.389416 38.41347 210-219
GTGGA 7293945 2.3853517 26.08546 100-109
CCGCC 9548040 2.3709583 34.813713 70-79
GCAGC 8590910 2.3695557 34.889957 30-34
ACAGC 7077490 2.3620524 27.49486 210-219
CAAAT 4710670 2.3540723 36.363148 200-209
CTACT 5087115 2.3407357 22.461695 50-59
CTTCA 5086560 2.3404803 50.884464 45-49
GGGCT 8272830 2.333693 42.688293 90-99
CTGTC 6120820 2.3275979 47.495594 30-34
GTATC 5208560 2.2962348 23.197939 200-209
CAGGC 8279115 2.283556 33.73899 70-79
GGCCG 10002520 2.2801042 24.788803 230-239
TATGC 5158765 2.2742825 20.547976 50-59
AAATG 4735770 2.2674909 34.5205 200-209
GCCTC 7371260 2.2651436 71.441536 4
TCCAA 5241920 2.2595773 42.1805 180-189
ACAAC 5594090 2.259031 21.13188 180-189
AATGA 4715490 2.2577808 26.50318 210-219
GCCTG 7569535 2.2286456 31.2324 210-219
TGGTG 6354735 2.2183568 33.303944 35-39
GCTCC 7213270 2.2165942 34.689125 80-89
GGGGG 10545770 2.2067807 97.58628 5
GTGGT 6237705 2.1775029 30.749294 120-129
TACAT 4062365 2.167006 18.270926 190-199
CAGTA 5223300 2.1572454 21.362217 45-49
CCTTC 5359525 2.1271925 55.485767 45-49
TGCAG 6214010 2.1210165 44.255028 30-34
GTGGG 7841695 2.1194215 30.533438 100-109
GTAGT 5012830 2.117383 25.183275 120-129
CCTCT 5320625 2.1117532 49.59841 35-39
TATCA 3910520 2.0860066 16.791769 170-179
GCAGA 6512900 2.082584 24.609762 140-149
AACAG 5379210 2.0812733 34.28008 210-219
GCTGT 5668375 2.06526 19.54698 230-239
TCTAT 3621130 2.061908 19.43026 110-119
GCTAT 4651260 2.0505447 32.662827 50-59
CAGAG 6368915 2.036543 33.276882 170-179
TGCAC 5642975 2.010307 26.186108 25-29
CTCAC 5400075 2.0078733 53.878128 20-24
GGCTC 6759020 1.9900113 19.546991 40-44
TGTGA 4703340 1.9866567 18.18456 210-219
ATGGA 5015250 1.9845635 12.291092 100-109
TCCGC 6441265 1.9793618 42.876686 70-79
AACTA 3959430 1.9786537 35.46456 130-139
CTGTA 4445160 1.959684 24.671824 190-199
TGAGC 5730815 1.9560885 17.202547 200-209
AGCAC 5837450 1.9481995 20.329086 130-139
TTCAG 4415845 1.9467602 47.647552 45-49
TCTCT 3963465 1.9467037 32.838055 35-39
AGAGT 4903745 1.9404403 29.850727 150-159
GGTCT 5297255 1.9300432 35.242416 15-19
CATAT 3613355 1.9274884 13.681389 160-169
ATATC 3611310 1.9263977 13.482453 170-179
CCGTG 6538825 1.9251808 19.573029 230-239
CAGAC 5764515 1.9238582 26.916721 140-149
GCCAG 6957725 1.9190887 35.96953 70-79
CCAGA 5749515 1.918852 34.985523 170-179
TGGGG 7066185 1.9098198 126.60581 4
TGGTA 4497330 1.8996396 30.352312 120-129
AGAGA 5109775 1.894219 29.0346 170-179
ACTAT 3545510 1.8912976 17.157085 130-139
AGGAC 5888570 1.8829463 21.178354 220-229
GCACA 5585910 1.8642502 18.338474 130-139
TCCAT 4049300 1.8632056 26.617228 45-49
GATGG 5684310 1.85895 20.774097 100-109
CTATT 3263755 1.8584151 18.824467 110-119
GGATC 5412775 1.8475325 18.021254 70-79
GTCAC 5175905 1.8439137 37.5238 160-169
ACCCT 4949340 1.8402796 42.53225 10-14
ACCAG 5507585 1.8381099 13.731754 190-199
GGTGG 6779225 1.8322613 17.672523 40-44
TGGGA 5592560 1.8289448 16.501848 100-109
GCCGA 6629255 1.8284897 19.658985 220-229
CAGTT 4144925 1.8273228 24.081116 190-199
CCTGC 5938840 1.8249696 49.02642 20-24
TAGTA 3563255 1.8211508 17.603624 120-129
GAGGA 5931035 1.8170896 27.502909 220-229
TTCCA 3940250 1.8130283 22.729525 180-189
CACAG 5429150 1.8119329 15.855355 140-149
ATACT 3386140 1.8062841 27.165468 130-139
TGTCT 3820780 1.7980207 48.90537 30-34
GCTGA 5217700 1.7809479 26.136839 200-209
TCAGC 4981695 1.7747265 11.813839 45-49
GCAAA 4559805 1.7642368 32.845474 200-209
CAAGG 5503555 1.7598329 17.201286 80-89
TTCTG 3709430 1.7456206 42.092144 30-34
TAGTG 4121315 1.7408136 25.885923 120-129
AGTCA 4210845 1.7390971 28.496523 160-169
CATGA 4203120 1.7359067 23.643742 60-69
ACCTG 4870445 1.7350937 30.082651 25-29
CCTGG 5853275 1.7233391 35.465286 2
GTGCA 5030660 1.717106 51.61062 25-29
TACAA 3431100 1.7146304 17.067453 130-139
CTGAA 4143820 1.7114155 22.68721 200-209
AGTTC 3881520 1.7111987 21.192698 190-199
TGACC 4794420 1.7080098 13.994456 210-219
CTCAG 4765085 1.6975592 78.20073 6
AACAC 4195860 1.6943915 22.90093 190-199
ATGCA 4075410 1.6831621 16.490559 60-69
GGATG 5144690 1.6824771 22.364817 100-109
AGGGG 6514180 1.6493891 33.513638 90-99
AGCTA 3965060 1.637587 25.242289 50-59
TCTGC 4292060 1.6321651 33.821453 200-209
ATTGG 3862120 1.6313316 19.726847 100-109
GGGGC 7425325 1.62173 29.088928 90-99
GGAGC 6131295 1.6203088 12.907664 200-209
CGATT 3672420 1.6190153 35.648636 160-169
TCTCA 3497070 1.6091079 26.490707 100-109
GGGCC 7039440 1.6046613 28.302317 2
GCCGT 5387795 1.5862912 28.855045 230-239
TCTGA 3594600 1.5847077 21.200426 220-229
ACAAT 3095415 1.5468777 23.055185 180-189
CAGCT 4314815 1.5371509 12.382067 50-59
AGTGA 3883910 1.5368857 86.25747 9
GTCCG 5205105 1.5325029 35.19872 70-79
GCACT 4295640 1.5303197 25.20711 25-29
CTCCC 4747615 1.5226908 13.421305 190-199
AGTTA 2976020 1.5210196 15.898074 50-59
CCATG 4260595 1.517835 15.63594 160-169
CGTCC 4926305 1.5138237 18.450638 180-189
GTTCT 3208985 1.5101161 22.262173 190-199
GGCTT 4107420 1.4965295 20.955296 25-29
CGGCC 6279710 1.4940566 31.230736 230-239
TTAGT 2736730 1.4930519 25.416744 110-119
TTCTC 3017190 1.4819293 20.992533 190-199
GAGCA 4601775 1.471477 7.6073794 200-209
ACTCC 3946270 1.4673148 17.433615 140-149
CATCA 3395585 1.4636977 31.280083 45-49
CTTTA 2554300 1.4544443 41.837635 45-49
TACGC 4047405 1.4418862 25.08695 140-149
GGGGT 5284805 1.4283557 121.327866 7
GTTAC 3234745 1.4260628 15.065732 50-59
CTGGT 3904450 1.4225777 31.092066 35-39
GTGAC 4142735 1.4140321 14.504356 210-219
CTCAA 3243280 1.3980453 18.491343 150-159
GGCAG 5242505 1.3854296 13.54559 150-159
GAACT 3335455 1.3775574 15.023879 190-199
CAACT 3188380 1.3743801 16.809803 130-139
GTCCA 3852495 1.3724496 28.211063 180-189
TAGCA 3310110 1.3670899 11.413546 130-139
CTACG 3834755 1.3661296 16.387226 130-139
GAGAG 4458815 1.366046 26.10266 220-229
TTATT 1929425 1.3595569 21.804232 240-247
GGCCT 4602045 1.3549482 68.24522 3
CAACC 3885150 1.3533192 15.225205 140-149
ATACA 2705045 1.3517973 22.460325 35-39
CGACA 4045225 1.3500596 16.339365 70-79
TGGAA 3398380 1.3447586 19.264803 120-129
AACCC 3847115 1.3400704 19.846865 140-149
AGCAG 4183530 1.3377378 12.54762 50-59
AAGCT 3229400 1.3337562 15.985735 200-209
ATCTA 2493005 1.3298551 20.159292 110-119
TCACT 2884720 1.327347 13.959026 190-199
CCATA 3072425 1.3243967 14.170519 160-169
ACAGT 3201665 1.3223015 12.15281 120-129
CGTGT 3621780 1.3195876 23.172346 230-239
CAATT 2470570 1.3178874 23.87845 180-189
CACGT 3651600 1.3008808 16.454834 180-189
ACATG 3149085 1.3005857 14.397355 190-199
ACAGA 3358825 1.299565 20.280067 140-149
TCAAG 3145495 1.2991031 22.685888 150-159
TCCGT 3410780 1.297036 13.71178 150-159
ATTAT 1961565 1.2948762 9.686019 50-59
TGTGT 2860755 1.2898557 15.18171 240-247
GTGCG 4569200 1.2889314 16.759676 240-247
CCTTT 2619175 1.2864394 47.885807 45-49
GTGGC 4558400 1.2858847 13.933809 40-44
AATTC 2400695 1.2806138 23.852102 180-189
AAGAG 3446110 1.2774901 18.311203 150-159
TGTCC 3350795 1.2742251 52.839874 15-19
TTACA 2384185 1.2718066 9.366235 120-129
ATATT 1925540 1.2710952 25.8608 240-247
AGAGC 3970060 1.269478 24.852526 220-229
ACATA 2538590 1.2686145 21.020693 130-139
TGCGA 3694865 1.2611614 14.05919 240-247
CCAGT 3523075 1.2550938 18.32271 190-199
AACCA 3107490 1.2548809 17.302217 190-199
CCTAC 3372340 1.2539144 17.158194 190-199
ACACA 3079525 1.243588 7.965116 130-139
CGGCT 4191805 1.234164 21.643202 230-239
AGACC 3689905 1.2314746 97.66706 9
CTTCT 2483455 1.2197789 22.800646 30-34
TGGAC 3565475 1.216997 15.704691 80-89
AGTCG 3540310 1.2084075 15.412599 150-159
ACGTC 3385565 1.206106 18.1981 180-189
GATTG 2854135 1.2055658 23.042048 100-109
ATCCG 3376425 1.20285 23.430191 70-79
TACCA 2767315 1.1928763 10.6554785 45-49
TGAGT 2812245 1.1878718 20.73709 90-99
ATTAG 2292980 1.1719235 25.357391 110-119
GTCGA 3427030 1.1697419 11.97014 150-159
AAGGC 3642400 1.1647044 28.52876 25-29
ACGCA 3489050 1.1644409 21.316736 140-149
GAAGT 2935175 1.1614658 13.470784 140-149
GGGTG 4282565 1.1574744 14.472038 60-69
ATCAT 2163465 1.154067 15.305436 110-119
GATCC 3239095 1.153926 23.267448 70-79
GGACT 3365950 1.1488935 12.68822 90-99
ATTCC 2481270 1.1417075 22.144539 180-189
TCGGA 3336635 1.1388875 91.4951 5
CTTCG 2991960 1.1377691 102.66339 3
AGGCC 4123370 1.1373131 13.917557 2
GATCT 2568240 1.1322289 9.200939 220-229
CGCTG 3831020 1.1279407 12.203044 190-199
CCGAT 3150680 1.1224284 24.212631 160-169
TCGAG 3269025 1.1158103 11.620664 150-159
GCAAG 3481185 1.1131539 16.776602 25-29
AGCTC 3121880 1.1121683 12.115753 210-219
ACAAG 2861115 1.1069957 17.409428 80-89
TTGGG 3164440 1.1046655 14.582352 100-109
AGGTC 3232295 1.1032733 18.017246 10-14
GAACC 3290060 1.0980297 10.117336 190-199
CGCAG 3963670 1.0932646 16.702381 140-149
GACCC 3790540 1.0912168 41.77626 10-14
CACAT 2521585 1.0869521 13.932638 130-139
CTTGA 2460925 1.0849181 21.967838 90-99
AAAAA 1994520 1.0825096 5.3243313 190-199
GCTTC 2844410 1.0816594 26.698017 2
TTCGG 2961240 1.0789213 98.45707 4
TTGAG 2551140 1.077583 20.450397 90-99
GTATA 2108090 1.0774277 10.027483 240-247
ATGGG 3285975 1.0746182 19.92371 100-109
GCCCC 4321080 1.0730056 12.223757 80-89
ATCAA 2134285 1.0665705 12.239304 110-119
ATGAG 2688680 1.0639262 20.525442 60-69
ACACC 3045050 1.060686 13.02238 40-44
CTCCG 3442595 1.0578886 10.198538 140-149
GCACC 3664985 1.0550722 10.904922 130-139
TAGAC 2539060 1.0486428 16.580141 170-179
TACAG 2535555 1.0471953 8.673483 120-129
CCCCT 3260140 1.0456165 9.393947 70-79
CAGCA 3127460 1.0437633 12.36955 45-49
GAAGC 3254975 1.0408204 12.478339 200-209
GAGCC 3768790 1.0395124 18.255232 220-229
AGATC 2514790 1.0386192 12.385352 210-219
CGTGA 3038770 1.0372177 20.785757 150-159
CTATA 1942760 1.0363353 13.276563 50-59
GACAG 3234345 1.0342237 15.65251 70-79
GGAGA 3370730 1.0326897 81.85599 7
TTTCA 1807330 1.029112 7.805607 100-109
GCCAA 3072955 1.0255729 15.54409 180-189
TGGCT 2814350 1.0254022 30.461868 40-44
CGAGG 3879365 1.0251944 14.963647 220-229
GGGAG 4045385 1.0242907 14.504586 5
ATGGT 2413980 1.0196476 9.3918705 120-129
AGCCG 3693410 1.018721 19.341366 220-229
AACTC 2346170 1.0113379 16.87201 190-199
TGAGG 3091330 1.0109631 11.912327 220-229
GCTCT 2645390 1.005977 16.66707 210-219
GTAGC 2946795 1.0058241 11.345322 50-59
ACCGC 3484950 1.0032438 11.099435 220-229
ATGAC 2419705 0.9993487 11.052108 160-169
ACTCA 2318180 0.9992725 12.893276 190-199
AAACT 1990755 0.99484384 16.63057 130-139
TTATA 1505355 0.9937209 13.22035 110-119
TAGTT 1813955 0.98962235 14.39169 50-59
CCGTC 3202410 0.9840812 10.357884 140-149
ATGAT 1908020 0.9751735 8.8914385 120-129
AAGGT 2461045 0.9738498 20.39018 15-19
CCGAG 3529970 0.97364086 18.11875 220-229
AACTG 2345290 0.96861494 12.414705 60-69
GATAC 2340935 0.96681625 24.253613 35-39
TTTAT 1367435 0.96355426 11.590739 240-247
TTTAG 1761105 0.96078944 29.930819 45-49
GCAGT 2804430 0.9572309 6.385693 50-59
CGAGT 2791110 0.95268446 14.870516 160-169
GGGAC 3591455 0.9491088 9.750345 90-99
GTAGA 2398155 0.9489638 16.238508 170-179
CATAC 2197090 0.9470756 14.211534 130-139
ACAGG 2959545 0.9463528 15.207733 70-79
ACCAA 2325830 0.93922734 16.40236 130-139
CGGAC 3377495 0.93158495 10.534298 220-229
GGTAT 2205215 0.93146676 9.166721 110-119
CAGAA 2407215 0.93137693 18.077034 140-149
GCCAT 2613995 0.9312346 7.589663 60-69
TAACA 1860420 0.9297113 8.843286 120-129
CCAAC 2655360 0.9249449 13.394901 130-139
TACAC 2138135 0.92166257 22.075747 35-39
TATTT 1303585 0.9185629 11.368073 240-247
AGCCC 3174470 0.9138632 11.363211 80-89
TCATA 1700190 0.9069401 8.800339 110-119
GTTAT 1661740 0.90657973 10.617614 110-119
AAGTT 1772200 0.90575695 17.436546 140-149
AATCA 1810835 0.9049321 13.367737 110-119
TATGG 2135575 0.9020514 11.800443 35-39
GAGAT 2272650 0.8993007 11.96213 210-219
CCCAG 3104415 0.8936958 14.432348 80-89
GATGA 2247150 0.8892103 7.0901117 60-69
TGGTT 1969755 0.8881222 8.962724 50-59
AGTCT 2006055 0.88438517 15.914094 8
GACTG 2565120 0.8755477 12.306403 90-99
GTTTC 1857580 0.8741585 5.4972787 100-109
CCCCA 2891320 0.86873686 16.59006 80-89
TCCTT 1757890 0.8634089 10.549947 1
AGAAG 2326830 0.8625675 15.712061 140-149
CGTAT 1945535 0.85770446 7.25198 240-247
CGGAG 3241420 0.8566056 70.819565 6
GTCTG 2342385 0.8534429 14.208636 1
ACGCT 2372785 0.84530354 15.008259 190-199
TCTTC 1718335 0.84398097 45.532505 2
ATAGT 1648125 0.8423433 15.405852 120-129
GCCTA 2358820 0.84032863 16.419073 190-199
GTCTA 1894565 0.835234 9.499557 30-34
CATGG 2446610 0.8350969 8.464808 200-209
GGTTC 2285010 0.83253837 11.30787 160-169
TTACC 1809320 0.83252287 9.72867 45-49
TTATG 1518850 0.82862467 12.050694 50-59
GGTAG 2524190 0.82549036 11.77398 120-129
ATCCA 1911715 0.82406217 9.50966 180-189
GATCA 1994580 0.82377017 9.042999 110-119
ATATA 1324645 0.8191832 11.165298 130-139
TGGCA 2379905 0.8123286 5.714102 100-109
GGTGC 2867640 0.80893606 11.574639 60-69
TCATC 1754310 0.80721116 10.260609 110-119
CTATC 1749480 0.80498874 7.980581 110-119
GCTTG 2201880 0.8022502 16.684105 90-99
CATTA 1502860 0.80167747 12.77383 110-119
ACATC 1855410 0.79979134 5.657552 110-119
ACAAA 1698620 0.7952237 12.847045 130-139
ATATG 1550955 0.7926805 5.5582824 50-59
TGATG 1869625 0.78971606 12.217282 120-129
TATAC 1466585 0.7823272 9.314363 130-139
CCTCC 2416620 0.7750765 10.117195 140-149
GTTCA 1730665 0.7629773 13.764313 160-169
CTGAC 2131640 0.7593957 9.451885 220-229
AACGC 2256730 0.7531645 15.216269 180-189
ACGCC 2611945 0.75192404 13.622686 180-189
TATGA 1469590 0.75109553 5.073447 50-59
CCAGC 2608605 0.75096256 6.55257 80-89
TCAAC 1741780 0.7508101 7.4786 110-119
ATCTT 1307405 0.7444497 12.52224 3
GGGAT 2261360 0.7395366 13.191899 100-109
TTGGA 1745080 0.7371091 6.521756 60-69
TATTG 1347770 0.7352902 6.8851595 240-247
CAACG 2192715 0.7318001 15.482267 180-189
TTTAC 1276815 0.72703135 13.309852 45-49
GCGGA 2750435 0.72685367 8.8142 220-229
GGTTA 1715795 0.72473925 7.9586186 50-59
TTTCT 1192140 0.7245969 9.468264 4
ATAAA 1238400 0.7174611 7.6120696 130-139
GACCG 2590270 0.71445155 8.917356 210-219
GACCA 2127915 0.7101736 6.108433 170-179
GGATA 1792485 0.7092967 15.708869 35-39
GGGCA 2683730 0.70922565 13.549441 150-159
CCCCC 2736200 0.7091525 13.155369 80-89
ATGCC 1978585 0.70487 8.086156 50-59
GATTA 1377185 0.70386803 5.6328917 50-59
AATAC 1405045 0.7021459 11.533063 130-139
TGCCA 1966580 0.7005933 7.276353 60-69
GAAAT 1461095 0.6995735 7.0071783 110-119
CGCCG 2912645 0.692971 10.690876 220-229
TCCGG 2352440 0.6926126 11.242596 70-79
CATCC 1856860 0.69042367 6.331234 110-119
CAGTC 1937380 0.69019073 5.6210637 210-219
CACAA 1707310 0.6894538 8.372513 130-139
TATAG 1337760 0.68371826 6.3860316 120-129
AAATA 1159650 0.6718377 12.748886 130-139
CCCGT 2174165 0.66810775 10.125411 140-149
AGATG 1679670 0.66465515 6.1087294 120-129
GCCGC 2792340 0.66434824 11.820167 220-229
CAAAC 1644410 0.6640532 13.4466505 130-139
AGGCA 2057320 0.6578547 6.5211153 80-89
GCGAC 2364150 0.65208286 7.158684 70-79
CACGC 2257170 0.6497918 12.195244 190-199
ACTTC 1398215 0.6433611 29.450119 4
TACTT 1129705 0.6432655 11.026404 1
GACGA 2008495 0.64224225 9.746797 220-229
AGCAA 1646110 0.6368974 5.0161924 50-59
TCAAT 1179255 0.6290554 8.135653 110-119
AAGCA 1612950 0.6240674 5.8315177 120-129
CACCG 2163930 0.62295 8.928541 45-49
AATAA 1075180 0.62290037 7.102652 130-139
AATGG 1556465 0.61590225 6.741916 120-129
TAGCT 1370945 0.60439193 12.443169 50-59
CCGGC 2508760 0.5968794 9.137392 70-79
AGTAA 1245600 0.5963943 5.431696 50-59
TCCAC 1603045 0.59604937 10.023515 180-189
CCCTA 1601515 0.5954805 11.638732 110-119
TTCAA 1115080 0.5948222 5.9069557 200-209
ACGAC 1778300 0.59349257 9.084147 220-229
TGATT 1085100 0.5919878 6.0821137 50-59
CACGA 1763440 0.58853316 8.609915 180-189
GGTGA 1793885 0.58665735 6.712808 120-129
ATTTC 1029250 0.5860654 6.7546964 240-247
GGCCC 2458465 0.5849133 10.057442 70-79
ATTAA 944525 0.58411044 6.9814878 110-119
ACCTA 1353610 0.58348584 7.404812 130-139
ACCCG 2025865 0.58320403 8.774163 140-149
CTTTG 1231515 0.57953864 7.2654543 45-49
GGGGA 2282570 0.5779463 9.340231 4
GTTCC 1515880 0.57645196 6.7351694 140-149
CCGTA 1605385 0.5719177 6.2430696 230-239
CGGTT 1526065 0.5560184 11.188321 160-169
TTAGC 1261105 0.5559681 9.645049 45-49
TCAGG 1627220 0.555416 5.2911325 150-159
TAATA 893120 0.5523207 7.7496085 130-139
GAAGA 1466440 0.54361665 6.4805803 15-19
AAGAT 1132860 0.54241437 5.871656 15-19
ACATT 1015880 0.5419055 6.611749 110-119
CTGTT 1150705 0.5415103 5.780923 240-247
CGGCA 1946030 0.53675646 10.531159 70-79
GGAGG 2117945 0.5362633 5.3004384 6
CTGCG 1810745 0.5331251 11.596782 25-29
TATGT 974500 0.53164876 6.9411807 140-149
GTCCT 1387330 0.5275676 6.0551944 45-49
CCACG 1829000 0.5265307 7.5559735 180-189
GCTAC 1465975 0.5222529 7.759955 50-59
GGGTT 1476810 0.5155355 6.3786097 100-109
AAATC 1020485 0.50996894 7.558999 110-119
AGTAT 978120 0.49990913 5.3113203 110-119
GTAAC 1207005 0.49849832 7.0119567 120-129
ACCCC 1641650 0.49325636 8.653507 140-149
TGGCC 1662890 0.48959315 14.751081 2
AACAT 978070 0.4887728 6.1047163 110-119
GGTTT 1078385 0.48622164 5.833531 100-109
TGACG 1423140 0.48575777 8.529413 220-229
TATCC 1052365 0.48422503 5.3206334 110-119
CGCGG 2114785 0.48207158 6.354434 220-229
CATAA 956750 0.47811854 6.196793 110-119
CAATC 1074075 0.46298978 6.0387535 110-119
CCGGT 1570965 0.46252835 8.709822 160-169
ATTCT 800220 0.4556534 7.2186027 2
ATGCT 1014135 0.44708943 6.7245684 50-59
CCGCG 1869825 0.44486523 6.5933223 220-229
TAAAT 714225 0.4416889 7.6397843 130-139
TGCTA 986555 0.43493056 5.929638 50-59
TGCGC 1458880 0.4295279 8.517422 25-29
GCCTT 1128105 0.4289907 8.688502 1
GCATG 1245340 0.4250696 8.439852 60-69
GGTCA 1236610 0.4220898 5.386878 160-169
CACTT 910420 0.4189118 6.896038 3
ATGGC 1202855 0.4105683 6.9649367 1
CTAAC 944965 0.40733573 5.6108665 110-119
TACCT 873295 0.4018295 5.0841813 2
GCGCT 1348040 0.39689407 7.678581 25-29
CCGGG 1738895 0.39638633 5.2992263 80-89
CCTAA 915185 0.3944988 7.7032967 110-119
AGCTT 892735 0.39356926 6.8722467 1
CATGC 1102075 0.3926137 6.9518228 60-69
ACGAG 1227425 0.39248502 7.4180646 180-189
TTTGA 718565 0.39202073 6.0807924 45-49
GGTAA 962590 0.38090244 7.3951874 120-129
GAGGC 1413240 0.37347496 5.493737 35-39
TTCTT 598140 0.36355662 18.978868 1
TGCTG 979310 0.35680947 14.654049 1
TGGGC 1232020 0.34754208 9.419179 1
ATGTG 820630 0.34662816 5.2462106 140-149
CGAGC 1187770 0.32761222 6.2485337 180-189
CTCTT 636610 0.3126787 8.599874 1
GCTTT 664125 0.31253058 5.1460195 45-49
CGGGA 1144825 0.30254135 5.028393 80-89
TCACA 700785 0.30207974 5.349909 5
GACTT 683130 0.30116326 6.818298 1
GCGAG 1135925 0.30018935 5.2935696 240-247
CGCTT 780975 0.29698566 5.7304444 1
ATTGT 542540 0.29598844 5.625805 240-247
AACTT 553200 0.29509598 10.348122 3
CTTTC 599770 0.29458427 5.086114 45-49
TTCCT 580440 0.28509012 6.3626013 4
GAGGT 866520 0.28337958 6.649707 7
CCCTT 696315 0.27636707 5.4419866 3
TAACT 508745 0.27138215 7.41941 2
GTCTT 568020 0.26730454 11.933998 1
AATCT 492915 0.2629379 7.6961308 2
TTGGC 720695 0.26258364 7.2112794 1
TGTAA 508120 0.25969598 7.943434 25-29
CGGGG 1183700 0.2585263 9.321025 3
CCTCG 773935 0.23782554 5.4596353 5
TGCTT 454770 0.21401021 11.120792 1
TCGGT 555625 0.20244074 5.905806 7
TCGCA 465900 0.16597667 5.412834 7
TAGGC 448270 0.15300718 6.706304 1
TTGCT 270145 0.12712753 5.7752585 4
Produced by FastQC (version 0.10.1)