FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_8_SL248354.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_8_SL248354.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11047110
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 12677770 8.639264 60.856697 110-119
ATACA 11655690 7.509569 37.064682 110-119
CACAG 16909100 7.420903 41.74029 110-119
TCCCT 19606075 6.903908 103.10374 30-34
CAGTA 14102000 6.545968 38.799545 110-119
AGTAA 10984575 6.354084 47.164917 110-119
GGAGA 17593390 6.2240224 95.69499 35-39
GGTGA 19553685 5.87125 101.911285 45-49
GTAAT 11911980 5.8483586 39.300488 110-119
CCCTG 19316325 5.7738376 67.75535 30-34
AATAC 8397210 5.4101844 39.043312 110-119
AGACG 13619465 5.3664737 103.18803 40-44
GTAGT 15012530 5.3103123 30.472927 80-89
ACAGT 11220715 5.208513 35.318703 110-119
GTGAC 14964525 5.004633 85.12024 45-49
GAGAC 12175600 4.7975483 143.02075 40-44
CCCCA 12177505 4.776671 101.6683 60-69
TAGTA 9612300 4.7192974 39.189636 80-89
CTCAG 12396320 4.6175265 34.14732 140-149
ACTCC 10939820 4.5387278 116.50215 30-34
GGCCC 17687360 4.487856 58.94303 60-69
ACCAG 10068585 4.4188037 91.66993 45-49
GCACA 9871035 4.3321047 33.839382 110-119
GTGTC 15173090 4.306889 23.660542 120-129
TGACC 11483305 4.2774363 145.44763 45-49
CGTGT 15054125 4.2731204 23.161549 120-129
AGTAG 10242075 4.2684855 31.776318 80-89
CACTC 10102775 4.1914535 95.459015 25-29
CTCCC 12227165 4.070738 67.14473 30-34
CCCAG 11338570 3.9931717 87.02052 60-69
GACCA 8908655 3.909745 104.88022 45-49
ATGCA 8401730 3.8999755 93.65871 25-29
TGAGG 12601230 3.7836845 69.90552 35-39
CCCTT 10686195 3.762941 39.424446 20-24
AGGAG 10590540 3.7466207 96.0707 35-39
GACGG 13019755 3.6961358 109.90892 40-44
ATGGT 10435385 3.69126 25.429085 190-199
ACGGT 10975825 3.6706796 97.35273 40-44
TACAC 7098090 3.6697962 26.797117 110-119
AGATA 6326265 3.6594605 19.843658 150-159
CTGAG 10879680 3.638525 105.1166 35-39
CCACT 8764115 3.636068 26.883028 240-247
TGTCC 11343255 3.5862012 20.5252 120-129
CAGGG 12602490 3.5776799 34.776695 50-59
GAGGA 9993960 3.5355682 126.45026 35-39
TGGTG 13610710 3.4686708 17.979239 25-29
GCCCC 12253545 3.4629397 73.24642 60-69
TGCAC 9245530 3.443883 112.46968 25-29
CTTGG 11952720 3.392785 22.620863 20-24
AGGCT 10116560 3.383313 28.371738 30-34
CCGTG 12488850 3.351623 23.235033 120-129
GGGTT 13081090 3.333698 41.00606 50-59
GGATG 10954790 3.289319 68.579094 20-24
CCTGA 8804310 3.2795322 82.225 30-34
TCTTG 9798060 3.2763772 17.824745 170-179
CTCTC 9262900 3.2617543 13.729718 130-139
CAGAT 6990475 3.2448893 17.984816 150-159
TCAGG 9652030 3.2279582 20.725224 140-149
CCAGG 10197935 3.2245169 39.382355 50-59
CTTCA 7312740 3.208935 16.837025 200-209
GCACT 8527480 3.1764152 86.97121 25-29
TCGCA 8379945 3.1214597 23.853338 100-109
TCTCA 7097865 3.114645 21.718332 130-139
CCTGG 11598115 3.1125772 28.89568 50-59
TGTCT 9268315 3.0992355 19.350174 180-189
CGCAC 8647655 3.0454957 24.423431 100-109
ACAGA 5565735 3.0439348 14.094719 140-149
GGTTG 11595775 2.9551675 71.85458 15-19
CGGAT 8834910 2.9546864 77.63983 20-24
GCCCT 9859370 2.9470613 21.146732 20-24
TTCCC 8250140 2.9051304 48.16258 50-59
TGGCC 10819310 2.9035702 43.11058 60-69
ACCAC 5859550 2.8642297 18.914898 230-239
TCTCT 7667885 2.8558586 15.166834 180-189
GATGG 9505685 2.8542066 21.93502 15-19
GATGC 8501535 2.843195 93.578865 25-29
GGTTC 9970880 2.830239 46.164497 50-59
AGGGT 9396085 2.821298 48.75608 50-59
GACTC 7493010 2.7910843 18.58105 190-199
CGGTG 11513935 2.7742732 68.81661 40-44
GTTGG 10765755 2.743638 51.89054 15-19
TCAAA 4252010 2.7395 56.400723 70-79
CGGCC 10739220 2.7248878 12.3989935 120-129
GCCGT 10148095 2.7234364 21.942791 120-129
TCACA 5213280 2.6953273 11.279531 140-149
TTCTT 6820655 2.6868544 19.930021 170-179
AGATG 6426360 2.678249 17.417381 180-189
TCCAT 5972105 2.6206455 19.456203 70-79
AGTCA 5630125 2.6134322 30.26418 70-79
TGGAG 8667465 2.60252 19.234406 25-29
GTTCC 8227080 2.601014 52.174194 50-59
CCAGT 6943305 2.5863235 34.941074 60-69
GGCTG 10725655 2.5843377 14.7729435 30-34
TGTTC 7500560 2.5081153 13.086524 140-149
TCCTC 7072275 2.4903672 16.872957 130-139
AGGGA 7025710 2.485489 8.849425 150-159
ACAGC 5593215 2.4546964 11.348404 120-129
GCGGA 8643625 2.4538102 85.524155 20-24
GAGAT 5877910 2.4496768 16.962523 180-189
AATAA 3016265 2.4217153 10.477138 110-119
GGCGG 11655150 2.383851 41.514496 20-24
ACTCT 5415125 2.3762345 13.0460615 200-209
CTGGA 7058415 2.3605676 14.785853 180-189
GTTCT 6963015 2.3283653 17.290909 160-169
CACGG 7354170 2.3253381 9.399016 120-129
TACCA 4487240 2.319956 15.621049 230-239
CATTT 4959120 2.301666 17.867538 150-159
TTGGG 9030095 2.3013074 77.866905 15-19
TGTAG 6465805 2.287119 7.7230973 210-219
CTGGC 8515055 2.2851787 33.903957 60-69
TCATT 4910655 2.279172 16.493744 150-159
GTGAA 5419530 2.2586422 18.135315 190-199
CAGCT 6062345 2.2581732 11.685825 150-159
TTCAT 4854790 2.2532434 16.162033 140-149
CAGAG 5645625 2.2245438 9.119467 140-149
TTCAC 5067510 2.2236962 18.629358 200-209
GATAC 4724295 2.1929574 14.808253 150-159
TTGTC 6546735 2.1891654 13.363418 170-179
ACTAC 4218285 2.180903 12.502802 230-239
GAATC 4689000 2.176574 14.781667 190-199
ATTTG 5162435 2.1512208 15.716002 150-159
CTACT 4862540 2.1337523 7.6784296 230-239
TGAAT 4328805 2.1252894 14.287168 190-199
CACTA 4096270 2.11782 14.7358885 240-247
TCAGC 5666140 2.11059 13.833934 140-149
TACAG 4478285 2.0787625 13.80671 160-169
CTCTG 6573035 2.078083 14.527987 180-189
TCTGC 6557105 2.0730467 11.916767 210-219
ATGTA 4220380 2.0720565 8.721077 70-79
CTCGC 6929275 2.0712276 16.226725 100-109
GCTGT 7264960 2.0621624 10.225671 140-149
GTCTC 6517020 2.0603738 11.930549 180-189
GTCCT 6480075 2.0486934 14.2969675 130-139
TTGGA 5789520 2.0478997 15.374377 170-179
TGGGG 9465375 2.047651 51.50883 15-19
ACACA 3350655 2.0410323 13.872882 110-119
ATATA 2991805 2.038765 10.732611 110-119
CAGGC 6423755 2.0311475 15.3140955 140-149
GTTCA 5133195 2.022373 15.147898 140-149
TCTCG 6351230 2.0079587 15.716317 100-109
TAGTC 5095270 2.0074315 24.899298 70-79
AATCG 4315095 2.003012 13.403673 190-199
TTGCA 5054475 1.9913588 14.217383 150-159
ATCTC 4519155 1.9830701 7.9503736 100-109
AATAT 2904590 1.9793327 13.333169 110-119
TTTGC 5908640 1.9757924 12.097662 150-159
GAACT 4255260 1.9752374 10.475172 160-169
AGCTC 5282810 1.967803 11.877827 140-149
TGGAA 4705010 1.9608591 9.101553 170-179
GAGTC 5837740 1.9523337 12.283593 210-219
ACGGC 6174225 1.9522477 14.196303 120-129
CCTTC 5540860 1.9511086 13.866967 200-209
GATAT 3942400 1.9355783 7.9285183 180-189
GTCAA 4165085 1.9333792 38.939934 70-79
AACCA 3155065 1.9218898 14.488659 70-79
TCGGC 7148310 1.9183865 7.834083 130-139
AAAAA 2027780 1.9182009 8.016531 8
CCAGA 4356185 1.9118006 29.525948 60-69
CAGCC 5393000 1.899285 10.330498 120-129
TACTA 3468675 1.8967977 10.28052 230-239
ACACG 4321360 1.896517 13.033635 120-129
ATATG 3856730 1.8935171 8.1714 180-189
CATAG 4019865 1.8659699 8.247002 210-219
GACGT 5504325 1.8408287 9.3363 170-179
AGTAT 3741140 1.8367668 11.887484 220-229
GGGGC 8913130 1.8230201 46.981438 15-19
CCTTG 5746075 1.8166374 25.647154 20-24
CTGTT 5431335 1.8161862 12.750624 140-149
GTTGT 5987340 1.7975477 6.797792 210-219
CGTAG 5331840 1.783144 9.770971 210-219
GGGCG 8692045 1.7778013 57.80626 20-24
GCAGA 4469985 1.7613066 14.528928 150-159
ATTGT 4164435 1.7353475 8.0326185 50-59
TGCAG 5175740 1.7309388 13.520998 150-159
AGTCT 4377285 1.7245603 12.925194 210-219
AGAAC 3153250 1.7245319 11.1039915 160-169
CACCA 3498105 1.7099226 13.146399 230-239
GTCCC 5617935 1.6792554 22.096605 50-59
ATAGT 3419535 1.6788701 7.2068696 220-229
CTCCA 4031210 1.672474 7.433496 150-159
GGTCA 4993625 1.6700337 7.4032683 130-139
CAAAA 2191305 1.6634109 21.787947 70-79
GTCAC 4453720 1.6589736 8.207121 130-139
TCCCA 3987110 1.6541777 10.622775 240-247
TTGGT 5470270 1.6423105 26.988348 25-29
TCAGA 3536945 1.6418046 9.96313 130-139
TGACT 4167225 1.6418008 11.178607 190-199
AATAG 2807630 1.624088 9.2921295 240-247
TCTGG 5708735 1.620427 11.966611 180-189
AACTG 3486165 1.6182333 10.378007 160-169
TGGTT 5350750 1.6064277 17.602995 10-14
GAGAA 3252265 1.5969505 10.294309 160-169
CTGTG 5603335 1.5905092 5.1645 210-219
GTCTG 5588500 1.5862982 8.933926 210-219
ATAGA 2737270 1.583388 9.864368 240-247
TTCAG 3992225 1.5728544 9.080455 150-159
TGTGC 5521890 1.5673912 7.8581076 220-229
GCTCC 5230305 1.5633891 6.0099535 150-159
CAGAC 3554345 1.5598968 17.829847 60-69
GAACC 3546770 1.5565723 11.9892845 70-79
ACTGA 3350035 1.5550433 8.352102 180-189
ACCAT 3002350 1.5522505 12.35055 45-49
TTGTA 3713015 1.5472379 8.583816 210-219
TGCTC 4881480 1.5432932 5.201814 150-159
GGCCG 6768145 1.5418361 12.846911 120-129
CTGGT 5411420 1.536034 6.720738 160-169
GTGCT 5404970 1.5342033 7.703314 220-229
TAGTT 3674955 1.531378 9.2655945 220-229
GCTGA 4568460 1.5278444 24.162567 30-34
GGACG 5372955 1.5253105 6.955959 170-179
CCACC 3883265 1.5232252 9.485315 230-239
CCCAC 3879410 1.5217129 10.407039 240-247
CAAAG 2764965 1.5121765 27.779856 70-79
GCTCA 4039155 1.5045518 10.693756 140-149
CCTCG 5000815 1.4947923 9.645926 130-139
AAATA 1848635 1.4842422 5.044279 9
GCTCT 4666390 1.4752921 9.936992 130-139
CATGT 3690850 1.4541188 8.677787 70-79
CGACT 3806060 1.4177257 8.23205 190-199
CACTG 3769410 1.404074 6.998564 230-239
GTGGA 4650810 1.3964666 24.270658 25-29
ACCGT 3744265 1.3947077 20.969997 45-49
TCGAC 3732495 1.3903233 8.966249 190-199
AATTG 2830030 1.3894441 9.180139 170-179
TATAC 2530565 1.3838049 6.8107 110-119
CTCTT 3696780 1.3768439 7.749579 200-209
TGATA 2803800 1.3765662 7.38885 180-189
GCGTA 4107525 1.3736924 10.335577 210-219
TGGAC 4105140 1.3728948 9.952828 170-179
CCTCA 3297735 1.3681688 7.271477 130-139
CCATC 3267535 1.3556395 10.677673 240-247
ACGGA 3438820 1.3549973 9.484153 200-209
GGGAG 5263330 1.3415239 6.360955 150-159
GTCCA 3581025 1.333902 18.253134 70-79
GAGGG 5232865 1.3337588 7.8891973 200-209
CTTGA 3383645 1.3330863 8.042223 200-209
GTAGG 4419505 1.3270143 5.0070376 160-169
CCATG 3559335 1.3258228 8.815839 70-79
ACTTC 3011345 1.3214214 6.675078 220-229
GGAGG 5140555 1.3102307 18.423805 30-34
CTGCT 4116495 1.3014412 5.448132 90-99
GAATT 2648400 1.3002703 10.097481 170-179
CTCGA 3490385 1.3001393 7.9724274 190-199
TATGG 3674780 1.2998626 6.005587 180-189
TCCAC 3129010 1.298168 9.157947 240-247
GTGTT 4303235 1.2919377 7.671324 160-169
AGCCG 4082205 1.2907652 9.211821 120-129
AGATC 2776360 1.2887509 6.7536206 130-139
CTGAT 3249170 1.280106 7.0365047 180-189
GGAGT 4237960 1.272503 7.0017095 210-219
ATCGG 3802710 1.2717522 8.049688 190-199
TTGGC 4461310 1.266345 8.808252 60-69
GTGGT 4965350 1.2654127 11.9904175 50-59
TGGTC 4428050 1.2569041 13.525885 50-59
GGCTC 4663005 1.2514071 8.30115 130-139
ATAAA 1555370 1.2487838 5.502458 7
ACGTG 3707155 1.2397954 8.988058 170-179
AAGTA 2137515 1.2364565 15.533222 70-79
ACTAT 2243075 1.2265949 6.1150975 230-239
ATAAC 1901595 1.2251663 7.253117 80-89
GGAAT 2925095 1.219062 8.470723 170-179
TGCCC 4055935 1.2123584 7.6522517 60-69
ACTCG 3222225 1.2002522 8.204312 190-199
GATCT 3046130 1.2001126 5.399497 130-139
GCATA 2562550 1.189503 7.8596706 210-219
AAAGT 2051670 1.1867992 20.289928 70-79
TTGAG 3343370 1.1826346 7.757991 200-209
TTCCA 2681290 1.1765885 7.371548 230-239
ACTGG 3504920 1.1721613 6.899331 160-169
ATACC 2265195 1.1711326 8.210604 70-79
CCATA 2246085 1.1612525 11.286245 70-79
ACTGT 2944730 1.1601629 5.3400455 230-239
TCACG 3114220 1.1600211 9.576983 200-209
CAATA 1788340 1.1521981 6.6931176 100-109
TATCA 2087180 1.141346 7.087629 70-79
AAAAG 1659700 1.1311468 14.841597 70-79
AGCTT 2867560 1.1297596 5.9236794 150-159
ATGGG 3742605 1.1237662 34.584656 15-19
AGTTC 2850130 1.1228926 5.485541 160-169
TCTGT 3349400 1.1200072 6.2436075 210-219
AGAGC 2785330 1.0975028 6.6808677 140-149
CGGCT 4085755 1.096491 7.8270245 130-139
CTCGG 4079670 1.0948578 8.276791 130-139
AGGTT 3089860 1.0929618 22.763344 10-14
GAAGA 2221710 1.09092 19.022556 35-39
AGAGA 2217965 1.089081 13.958364 35-39
TATGT 2573225 1.0722798 7.4313717 220-229
ATCCA 2068255 1.0693123 14.720562 240-247
TCTAC 2423670 1.0635412 6.867588 180-189
GTCTA 2692985 1.0609804 7.0871034 180-189
ATATC 1930725 1.0557905 7.533597 70-79
CTGCC 3498395 1.0457042 5.673544 200-209
CTGCG 3885690 1.0427996 6.858807 210-219
CTGAA 2236565 1.0381849 15.41688 35-39
TACTG 2626645 1.034844 6.051134 180-189
GCTTC 3269435 1.033641 5.2373056 150-159
GAGCT 3086350 1.0321777 5.9826713 140-149
AACTT 1886990 1.0318748 5.8098536 200-209
CTACC 2464395 1.0224315 8.194225 230-239
CATGG 3041230 1.017088 5.195877 190-199
AAAAT 1262800 1.0138838 5.0854273 8
TCCCC 3041550 1.0126103 5.263879 80-89
GCGGT 4184670 1.0082928 5.6520414 130-139
TAGAT 2045810 1.00442 7.589015 240-247
TGCGT 3512610 0.99705595 8.188032 210-219
ATACG 2140620 0.9936484 5.133518 120-129
TATAA 1452620 0.9898877 6.427624 240-247
GGGGT 4527630 0.9794653 7.4785237 10-14
CGTGG 4046505 0.97500205 8.286271 50-59
CTTCT 2616270 0.97441435 5.15721 210-219
ACAAT 1503960 0.96897674 7.6692257 100-109
CGGAG 3406210 0.9669778 6.569458 210-219
CCATT 2187405 0.95986474 6.0727563 50-59
CAGCG 3030420 0.958198 6.1987066 160-169
CGAAC 2182835 0.9579816 12.573258 70-79
ACCCA 1950225 0.9532972 12.531848 240-247
GAGTT 2662520 0.9418008 5.267281 160-169
TAGGC 2803945 0.9377321 5.6014137 160-169
AAGAG 1908940 0.93734133 14.121842 35-39
ACGTC 2509680 0.934835 12.489899 70-79
GAGGC 3289480 0.93383974 15.664692 30-34
TGAAG 2236895 0.9322479 10.313384 35-39
CCCAA 1903970 0.93068707 9.204351 240-247
GGTGG 4288750 0.9277882 24.196646 25-29
ACTAA 1430840 0.92186666 12.082795 240-247
TGTAA 1870940 0.918565 6.4641514 230-239
TGCAT 2305130 0.9081737 5.983389 210-219
CATCA 1729725 0.8942883 5.702856 230-239
TCGAA 1899510 0.88172823 16.25738 70-79
GGGCC 3856325 0.8785009 18.311445 15-19
ATCAA 1360130 0.87630934 13.501639 70-79
TTCTG 2591635 0.8666179 5.1761513 210-219
CCAAT 1657705 0.8570531 10.074824 240-247
CGTCC 2829075 0.84563804 10.216106 70-79
TGAAC 1807095 0.83883035 6.3447123 190-199
AGCGT 2505930 0.83806604 6.3558135 160-169
CCAGC 2360665 0.83136946 5.5923376 80-89
CGGTC 3091620 0.8296957 5.3422537 130-139
TGGGC 3438725 0.82855797 19.848726 15-19
GACCG 2607720 0.8245431 19.384092 45-49
GTATG 2324460 0.8222203 5.20164 220-229
CTTCC 2320180 0.8170073 5.1314545 230-239
GGTCC 3031700 0.813615 13.744271 50-59
AACCG 1797880 0.78903633 5.6913753 190-199
TAACC 1520520 0.7861268 5.8499618 80-89
TGAGA 1864645 0.7771091 5.7067595 240-247
AAAGA 1127020 0.7681057 5.7377915 8
CATAC 1450850 0.7501066 9.766876 70-79
AAAGG 1508155 0.74054515 8.632724 10-14
CTAAT 1349825 0.7381333 9.380731 240-247
AGCCA 1679610 0.7371311 5.8739843 80-89
AATCC 1407480 0.7276838 8.922191 240-247
CCTTT 1941990 0.72328275 5.1556377 50-59
ATGAT 1459190 0.7164104 6.5275793 240-247
GTCGA 2136505 0.714518 11.453224 70-79
GTAGC 2118885 0.7086253 5.493346 80-89
AAAGC 1273900 0.69670385 10.064158 70-79
AAGGT 1628040 0.67850167 9.6109295 10-14
CATTG 1718325 0.67698467 5.5666676 50-59
AGGAT 1602690 0.6679368 6.609071 10-14
CGATG 1969070 0.6585222 15.049712 15-19
GGAAG 1861050 0.6583845 6.5887856 30-34
TAGGT 1857655 0.65709966 5.389383 10-14
AGAAG 1306600 0.64157605 5.8085012 9
GAGGT 2128945 0.6392437 6.751002 10-14
CCGTA 1707205 0.63591963 5.3634877 80-89
TATGA 1294355 0.6354823 5.5149627 240-247
CAATC 1210495 0.62584025 8.612061 240-247
ATAGG 1467400 0.6115533 5.15338 10-14
TAAAA 726845 0.5835732 5.688379 8
CATCC 1402435 0.58184415 7.180842 240-247
AATGG 1385660 0.57748747 6.520651 10-14
AAATG 979365 0.5665187 5.359577 9
AAGAA 827505 0.56397516 5.3547673 8
GGGTC 2320865 0.55921054 5.468448 70-79
GAAAA 809870 0.5519563 6.005488 8
GAAGG 1526535 0.54004306 5.6181226 10-14
AAGCA 972205 0.5317049 6.7461796 70-79
AAGGG 1454305 0.51449025 6.3062987 10-14
ACGAT 1068825 0.4961349 6.8069773 10-14
TGATC 1252575 0.49348876 5.0771017 240-247
TTGCC 1551525 0.49051887 5.373313 60-69
ATAAG 833445 0.4821105 5.2466984 9
GGTCG 1988520 0.47913226 5.537064 70-79
GATCC 1270665 0.47331226 5.128944 240-247
CGGTT 1564900 0.44419762 7.937942 10-14
GAAGC 1122440 0.44227472 5.3221536 30-34
TAAGG 1042070 0.43429288 5.8363457 10-14
TACCG 1159165 0.43177935 5.157327 70-79
AAGCT 910420 0.42260534 8.8696 30-34
TAAAG 695800 0.40248907 5.421731 9
Produced by FastQC (version 0.10.1)