FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_16_SL248345.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_16_SL248345.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 7164785
Filtered Sequences 0
Sequence length 251
%GC 54

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33752 0.4710818259026614 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26570 0.3708415535148647 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24895 0.3474633223467278 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22768 0.3177764580514279 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22186 0.30965339504255884 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22176 0.30951382351319684 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22157 0.309248637607409 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 21114 0.2946913270949512 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20853 0.2910485101786027 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20529 0.28652639262727353 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20101 0.28055273117057944 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19701 0.27496986999609896 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18514 0.2584027294608282 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 18241 0.2545924267092453 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18217 0.25425745503877645 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17700 0.24704160697076047 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17523 0.24457119090105284 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17513 0.24443161937169086 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17482 0.2439989476306686 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17035 0.2377601002681867 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16306 0.22758533577769605 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16289 0.22734806417778064 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16109 0.22483577664926443 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15650 0.2184294434515481 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15463 0.21581945585247847 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15319 0.21380962582966553 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14965 0.20886879369025033 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14842 0.20715206387909757 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14725 0.20551907698556204 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14462 0.20184834576334112 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14452 0.2017087742339791 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 14365 0.2004945019285296 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14094 0.19671211348281908 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13769 0.19217603877855371 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13446 0.18766787838016075 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 13445 0.18765392122722455 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13289 0.18547660536917715 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12982 0.18119175941776341 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 12831 0.17908422932439702 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12548 0.17513435504345212 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12522 0.17477146906711086 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 12519 0.17472959760830226 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12496 0.17440858309076965 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 11902 0.16611803424666616 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11851 0.1654062194469199 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 11393 0.15901384340213975 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11324 0.15805079984954187 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11022 0.15383573966280914 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 10728 0.149732336699566 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10608 0.14805747834722185 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 10578 0.14763876375913582 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10415 0.14536374783053505 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10328 0.14414947552508553 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10161 0.14181863098473996 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 10139 0.14151157362014352 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 9655 0.13475631159902215 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 9552 0.13331872484659343 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 9300 0.12980152230667075 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 8992 0.1255027192023208 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 8882 0.12396743237933867 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 8702 0.12145514485082246 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 8217 0.11468592567676492 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 8133 0.113513524830124 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 8104 0.11310876739497418 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7905 0.11033129396067014 No Hit
TTGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 7830 0.10928450749045505 No Hit
AAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 7614 0.10626976245623561 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 7481 0.10441346111572086 No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG 7314 0.10208261657537526 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 14530620 7.658265 65.37526 35-39
TATTA 7177785 7.3013263 126.45531 240-247
TGGAT 10975525 7.1760573 78.02603 35-39
TCACC 11273340 6.447216 74.671524 160-169
TCTCC 9902985 6.079065 84.89644 20-24
CTGTG 10484615 5.931299 86.39959 25-29
TCCCT 9568270 5.873596 74.21147 15-19
AGTGG 11386890 5.761224 40.370586 100-109
CTGAG 10581680 5.577004 50.249516 15-19
TGTAT 6557865 5.540819 59.389336 240-247
TTACT 6057650 5.331536 111.54584 240-247
ATTAC 6084360 4.988982 105.74857 240-247
TGGGT 9009650 4.892926 45.43752 100-109
CCTGA 8816900 4.840596 51.746796 15-19
CTCTG 8000485 4.714659 73.85916 35-39
TTCAC 6556035 4.651308 73.85541 40-44
ACACG 9063030 4.635593 49.135113 230-239
GACAC 8990080 4.59828 46.058544 230-239
CTCCA 7999405 4.574855 41.101448 80-89
TGAGA 7201520 4.386657 93.096245 15-19
ATTCA 5279515 4.329035 111.98471 40-44
TACTG 6332790 4.3131275 78.99461 240-247
TCTGG 7563695 4.2788925 135.10669 35-39
AAGGG 9061830 4.271442 39.685513 90-99
CCCTG 8863475 4.210404 84.90007 10-14
TGGAG 8250265 4.1742415 38.7815 90-99
CAGCC 9302660 4.116952 55.294228 30-34
GAAGG 8723165 4.1118064 31.19038 90-99
AGCCT 7461550 4.096491 50.296757 30-34
CCAGG 9615510 4.0851154 53.529877 80-89
ACCAT 6165455 4.0751905 67.58975 160-169
TCAGT 5974000 4.0687633 92.92921 7
GAGAC 8175755 4.0144224 88.52456 8
ACTAC 6027030 3.9836955 35.979893 130-139
CACCA 7439465 3.9637861 57.25387 160-169
GTATT 4671275 3.9468164 69.46729 240-247
TACTA 4787095 3.925266 28.65057 130-139
TCCAG 7139890 3.9198952 40.441093 80-89
GGGTC 8880655 3.8876886 121.497025 8
CACCT 6619910 3.7859223 44.9579 20-24
ACTGT 5506615 3.750437 67.929276 240-247
CTGGG 8545890 3.7411382 129.119 3
GACTC 6769760 3.7166889 51.94844 20-24
GATTC 5454100 3.7146702 59.713604 40-44
TGAAG 6093805 3.7119153 39.42821 150-159
CAAGA 6206635 3.66903 44.666576 180-189
AGACT 5780575 3.6678975 67.312195 15-19
GGACA 7287230 3.578143 27.644346 220-229
GCTGG 8157650 3.5711782 33.248947 90-99
ACTGG 6715000 3.5390954 44.363346 60-69
CCTGT 5991110 3.5305412 61.938843 25-29
GAGTG 6773510 3.4270735 39.174603 100-109
ACTCT 4773980 3.3869941 68.51956 20-24
TATCT 3803440 3.347532 49.051624 200-209
GGATT 5050560 3.302175 56.998753 40-44
TCCTG 5596960 3.29827 62.629658 20-24
AGACA 5553190 3.2827485 38.273914 170-179
AGCTG 6223545 3.2800775 37.138187 60-69
GGTCC 7058620 3.2188659 138.92862 9
CTGCA 5850495 3.2120001 46.38271 25-29
CTCCT 5224670 3.2072258 91.89177 20-24
CATCT 4442400 3.1517482 33.04729 170-179
CCATC 5466925 3.126531 36.000378 45-49
GTCTC 5252925 3.0955312 33.603565 30-34
GTCCC 6461395 3.0693471 74.09099 10-14
CCTCA 5331915 3.0493188 78.57131 5
GGAGT 5928900 2.999741 37.16525 90-99
AAGAA 4350170 2.9721074 38.082317 180-189
GGCTG 6687500 2.92759 34.21202 90-99
AGGGA 6181695 2.913843 40.222237 80-89
AGTAG 4765935 2.9030707 29.150774 50-59
GAGCT 5465575 2.8805945 30.303057 60-69
TGTGC 4976810 2.8154538 56.691494 25-29
GTGTA 4303995 2.8140535 36.241028 230-239
ATCTC 3939335 2.794839 47.960594 170-179
GGAAG 5911830 2.7866375 30.013634 80-89
ATCTG 4052290 2.759928 36.958557 200-209
GTGAA 4520350 2.7534778 38.657486 150-159
CAGGG 6685900 2.7268124 36.30253 80-89
CTATG 3970535 2.7042463 28.111135 50-59
GACAA 4552255 2.6910493 24.748869 180-189
ACCTT 3784460 2.6849597 70.226135 40-44
GAACA 4530075 2.6779375 34.77442 210-219
GGGAA 5601250 2.640241 33.592 80-89
CCCTC 5241295 2.593552 28.08974 20-24
ATGAA 3521515 2.5824914 27.839262 210-219
GAGTC 4899045 2.5820086 28.667496 150-159
AGAAC 4342140 2.5668404 30.59736 190-199
CACGG 6006980 2.5520444 44.14488 230-239
TATAT 2487885 2.5307055 24.408096 240-247
ATCAG 3975455 2.5225105 27.576899 45-49
TGCAA 3948120 2.505166 35.562527 200-209
CGCCA 5650995 2.5008843 39.604588 70-79
CAGTG 4736445 2.4963114 71.89105 8
CCAAG 4872405 2.492156 39.13104 180-189
AGGGC 6084700 2.4816155 32.07068 150-159
CTACA 3704405 2.4485064 18.992376 190-199
ACGGC 5738825 2.4381196 48.870316 230-239
AGGCT 4618650 2.4342282 20.212914 80-89
CTGTC 4115080 2.425003 50.29552 30-34
GTGGA 4787025 2.4220068 27.063791 100-109
TCTGT 3287635 2.4034321 25.550724 210-219
CACTG 4350530 2.3884993 20.331648 30-34
GCAGC 5597100 2.3779082 34.710457 30-34
CTACT 3338380 2.3684795 23.319822 50-59
CCGCC 6171995 2.3633664 33.905346 70-79
TCCAA 3548280 2.345312 44.301792 180-189
CTCTC 3803230 2.3346577 96.69614 20-24
TATGC 3425815 2.333249 21.379166 50-59
GTATC 3424335 2.3322413 23.205399 200-209
TGAAC 3660850 2.322887 37.71579 210-219
GTGGT 4273230 2.3206894 31.246336 120-129
ACAGC 4486355 2.2946978 26.92312 210-219
CTTCA 3228005 2.2901719 50.970257 45-49
GGGCT 5220945 2.2855756 42.228672 90-99
TGGTG 4195240 2.2783349 35.043957 35-39
CAAAT 2978725 2.2754977 34.69845 200-209
GGCCG 6370820 2.2481616 25.107107 230-239
CAGGC 5277595 2.2421677 32.58231 70-79
CAGTA 3523020 2.2354307 22.00246 45-49
TATCA 2707165 2.2197895 17.651793 170-179
AATGA 3014555 2.2107139 26.203362 210-219
GCTCC 4653330 2.2104647 33.892235 80-89
GCCTC 4648385 2.2081156 68.97705 4
ACAAC 3566980 2.1965058 19.337667 180-189
AAATG 2989285 2.1921823 32.913334 200-209
GCCTG 4779450 2.1795208 30.250694 210-219
CCTTC 3496190 2.1461775 56.190224 45-49
GTGGG 5087595 2.138072 29.978436 100-109
GGGGG 6559675 2.1332474 91.89662 5
GCTAT 3104715 2.114555 34.268677 50-59
TACAT 2575640 2.111943 17.718386 190-199
GCTGT 3729830 2.1100192 19.36122 230-239
GTAGT 3214195 2.1015165 24.966486 120-129
TGCAG 3962270 2.088288 43.758263 30-34
TCTAT 2369935 2.0858574 20.823755 110-119
GCAGA 4232065 2.0780094 24.381292 140-149
CCTCT 3359065 2.062002 48.872932 35-39
TGCAC 3730120 2.047886 27.290575 25-29
CTCAC 3554410 2.032765 56.53416 20-24
AACTA 2651400 2.0254486 37.38342 130-139
AACAG 3418015 2.0205474 33.50114 210-219
AGCAC 3920275 2.005157 21.232834 130-139
AGAGT 3289140 2.0035117 30.749514 150-159
TGTGA 3048005 1.9928573 19.82295 210-219
ATATC 2418280 1.9829129 14.230372 160-169
GGCTC 4346900 1.9822696 20.477716 40-44
TGGTA 3023390 1.9767637 30.974813 120-129
TGAGC 3725530 1.9635158 18.031246 200-209
CATAT 2388855 1.9587853 15.026062 160-169
CAGAG 3979190 1.953844 32.17535 170-179
ATGGA 3195660 1.94657 12.082953 120-129
TTCAG 2854145 1.943897 47.585857 45-49
TCTCT 2549515 1.9415228 33.44313 35-39
TCCGC 4084930 1.9404584 41.865334 70-79
CCGTG 4246365 1.9364238 20.068975 230-239
GGTGG 4603175 1.9344935 18.994074 40-44
CAGAC 3779020 1.9329073 26.51717 140-149
CTGTA 2823935 1.9233214 24.17724 190-199
GGTCT 3364015 1.9030722 34.73628 15-19
GGATC 3598030 1.8963174 18.616056 70-79
CAGTT 2759725 1.8795893 25.347942 190-199
ACCAG 3661150 1.8726188 14.244099 190-199
ACTAT 2283215 1.8721639 17.157633 130-139
GCCAG 4402880 1.8705481 35.421085 70-79
GCACA 3656070 1.8700205 18.49613 130-139
ACCCT 3266840 1.8683038 44.574337 10-14
TGGGG 4437185 1.864736 119.44735 4
AAAAA 2264085 1.862305 11.584032 90-99
CCAGA 3639875 1.861737 33.742092 170-179
GTCAC 3383250 1.8574495 38.104984 160-169
CCTGC 3903675 1.8543571 48.989902 20-24
GCTGA 3508780 1.8492788 27.243492 200-209
TCCAT 2606070 1.8489277 27.821539 45-49
TGTCT 2522440 1.8440348 51.052452 30-34
CTATT 2094425 1.843372 20.288456 110-119
AGAGA 3219435 1.826998 27.515265 170-179
TGGGA 3588830 1.8157772 16.229752 100-109
AGGAC 3695025 1.8143145 19.879791 220-229
GATGG 3585170 1.8139253 20.411669 100-109
ATACT 2197815 1.8021386 26.77405 130-139
TAGTG 2739295 1.7910155 26.453083 120-129
TTCCA 2516350 1.7852741 23.599873 180-189
TCAGC 3242485 1.7801677 11.073518 45-49
GCCGA 4174745 1.7736257 19.190386 220-229
TACAA 2314110 1.7677873 18.495953 130-139
ACCTG 3214335 1.7647129 31.931519 25-29
GAGGA 3740570 1.7631788 26.131912 220-229
CAAGG 3574435 1.755103 17.035528 80-89
CACAG 3428475 1.7536093 15.379619 140-149
AGTCA 2757705 1.7498224 29.502008 160-169
AGTTC 2533290 1.7253693 22.6749 190-199
TGACC 3136820 1.7221563 14.914316 210-219
AGCTA 2712030 1.7208405 26.421701 50-59
CTGAA 2709605 1.7193019 23.87332 200-209
GCAAA 2899325 1.7139256 32.153675 200-209
CCTGG 3747445 1.7089068 34.23041 2
TAGTA 2168295 1.7067848 16.553123 120-129
ATGCA 2671830 1.6953329 16.92677 60-69
TTCTG 2314360 1.6919175 41.655483 30-34
GGAGC 4141235 1.6889827 12.702256 200-209
GTGCA 3190110 1.6813263 49.4492 25-29
CTCAG 3058940 1.679399 75.13482 6
CATGA 2643705 1.6774868 22.83244 60-69
ATTGG 2538765 1.6599045 21.466698 100-109
AACAC 2650110 1.6319077 22.710728 190-199
GCCGT 3575655 1.6305672 29.166277 230-239
CTCCC 3258520 1.6124148 14.50959 190-199
TCTCA 2267505 1.6087261 26.313314 100-109
GGATG 3175875 1.6068414 21.60203 100-109
TCTGC 2724145 1.6053298 33.391727 200-209
AGGGG 4099880 1.6052026 32.816902 90-99
CAGCT 2911145 1.5982575 13.163872 50-59
GCACT 2886085 1.5844992 26.283077 25-29
GTTCT 2158200 1.5777564 23.962185 190-199
GGGGC 4643680 1.5731055 28.557045 90-99
CGATT 2306960 1.5712209 34.867546 160-169
GGGCC 4448220 1.5697064 26.586803 2
ACAAT 2045750 1.5627824 23.609354 180-189
TTCTC 2051475 1.5622523 22.990767 190-199
CGTCC 3282725 1.5593882 19.635952 180-189
AGTTA 1960460 1.5431864 16.09814 50-59
TCTGA 2264725 1.5424557 20.830627 220-229
CATCA 2273830 1.502937 32.04897 45-49
CCATG 2732785 1.5003356 15.417563 160-169
CGGCC 4080795 1.5000772 31.43157 230-239
GAGCA 3034140 1.4898098 7.4531627 200-209
GTTAC 2184050 1.4875096 16.847923 50-59
GGCTT 2619935 1.4821353 20.620792 25-29
CTGGT 2614375 1.47899 32.099693 35-39
GTCCG 3240910 1.4779171 34.008785 70-79
AGTGA 2411075 1.4686563 82.85321 9
TTAGT 1732525 1.4638312 25.380474 110-119
ACTCC 2552040 1.4595101 17.140932 140-149
CTTTA 1643665 1.4466431 40.88982 45-49
CTCAA 2167295 1.4325204 19.152012 150-159
GTGAC 2703825 1.425033 15.810788 210-219
GTCCA 2594625 1.4244839 29.602665 180-189
AAGAG 2509200 1.4239465 19.607323 150-159
GGCAG 3478540 1.4187059 13.261313 150-159
TACGC 2582190 1.4176569 24.295631 140-149
TAGCA 2226335 1.4126568 12.238046 130-139
CAACC 2646870 1.4102663 15.6922 140-149
AAGCT 2205160 1.3992208 17.47978 200-209
CGTGT 2463455 1.3936124 23.95941 230-239
AACCC 2606535 1.3887756 20.78987 140-149
GGGGT 3288935 1.3821816 114.31333 7
CAACT 2062225 1.363072 18.439554 130-139
CTACG 2459780 1.3504522 15.926399 130-139
GAGAG 2864800 1.3503703 25.63097 220-229
CAATT 1646185 1.3498194 25.066042 180-189
GGCCT 2949090 1.3448416 66.04479 3
CACGT 2447510 1.3437158 16.986813 180-189
TGGAA 2201565 1.3410378 20.004734 120-129
AGCAG 2721290 1.3361956 12.630539 50-59
ATCTA 1628895 1.3356422 21.263872 110-119
TCAAG 2094930 1.3292775 23.980282 150-159
TGTCC 2246350 1.3237667 55.638866 15-19
GTGGC 3017600 1.3210162 14.816979 40-44
AACCA 2141015 1.3184128 17.752567 190-199
GAACT 2077065 1.3179418 14.142495 190-199
ACAGT 2076670 1.3176912 12.373888 120-129
TTACA 1600200 1.3121133 10.007974 120-129
CGACA 2564625 1.3117641 15.693329 70-79
AATTC 1597345 1.3097723 25.065737 180-189
CCATA 1979975 1.3087072 15.533687 160-169
AGAGC 2657360 1.304805 24.736172 220-229
TGTGT 1855720 1.3023392 15.069999 240-247
AGACC 2513940 1.2858396 100.962845 9
GTGCG 2936890 1.2856838 17.02341 240-247
CCAGT 2341525 1.2855285 19.445333 190-199
TCCGT 2177565 1.2832319 14.162668 150-159
CCTTT 1683780 1.2822427 47.95974 45-49
ATCAT 1562380 1.2811021 16.359224 110-119
ATACA 1671160 1.276627 21.250051 35-39
TTATT 1163875 1.2707752 19.372072 240-247
GATTG 1933850 1.2643967 24.453026 100-109
ACAGA 2137895 1.263809 19.542643 140-149
ACATG 1988770 1.2619168 13.946051 190-199
ATCCG 2294315 1.2596097 24.42579 70-79
TCACT 1775055 1.2593478 12.743414 190-199
ACGTC 2293220 1.2590085 18.822453 180-189
ATATT 1235240 1.2565005 26.09117 240-247
TGCGA 2365700 1.2468262 14.038893 240-247
ACATA 1629085 1.2444851 20.777037 130-139
AGTCG 2344780 1.2358006 16.833418 150-159
TCGGA 2336020 1.2311835 95.19976 5
CGGCT 2691330 1.227298 21.213762 230-239
ATTAT 1205945 1.2267013 9.607535 50-59
CCTAC 2144535 1.2264582 16.631147 190-199
CTTCT 1572535 1.1975268 22.824902 30-34
GTCGA 2268030 1.1953499 13.072979 150-159
GATCC 2174550 1.1938571 24.283068 70-79
CTTCG 2021395 1.1912014 107.346054 3
CGCTG 2611240 1.1907755 12.84878 190-199
GATCT 1728905 1.1775202 9.191239 220-229
TGAGT 1791310 1.1712008 20.298992 90-99
ATTCC 1649795 1.1704795 23.168905 180-189
ATTAG 1486580 1.1701694 25.010605 110-119
ACACA 1900220 1.170134 7.6182795 130-139
TGGAC 2218780 1.1693932 15.533851 80-89
TACCA 1762075 1.1646814 10.658827 45-49
AGCTC 2109925 1.158377 12.751223 210-219
GGGTG 2739490 1.1512762 14.15762 60-69
GACCC 2598740 1.1500891 44.01659 10-14
TCGAG 2177760 1.1477739 12.701017 150-159
GAAGT 1878340 1.1441519 12.974925 140-149
ACGCA 2236335 1.143849 20.815117 140-149
AGATC 1801330 1.142982 12.254451 210-219
AAGGC 2327520 1.1428485 27.967827 25-29
TTGGG 2099030 1.1399331 15.9856825 100-109
TTCGG 2009545 1.1368289 103.2889 4
GAACC 2219685 1.1353328 11.05183 180-189
GGACT 2148105 1.1321442 13.371274 90-99
GCCCC 2940995 1.1261592 12.599335 80-89
GTATA 1427230 1.1234517 10.507526 240-247
AGGCC 2632480 1.1183999 13.678369 2
GCAAG 2271215 1.1152017 16.627796 25-29
GAAGC 2248010 1.1038078 13.624477 200-209
CCCCT 2228455 1.1027073 10.113737 140-149
ACAAG 1855725 1.0970051 17.029842 80-89
CGCAG 2579255 1.0957874 16.367702 140-149
AGGTC 2076750 1.094537 17.55992 10-14
TAGAC 1722250 1.0928042 18.242153 170-179
GCACC 2465480 1.091114 11.466349 130-139
CCGAT 1977045 1.0854242 23.491844 160-169
ATCAA 1413630 1.0798956 12.993513 110-119
CACAT 1630850 1.0779455 13.912375 130-139
GGAGA 2270715 1.0703386 84.84708 7
GCTTC 1811575 1.0675552 27.687626 2
GTAGA 1745540 1.0632596 17.757679 170-179
TTGAG 1623730 1.061633 20.047232 90-99
TGGCT 1875695 1.061108 32.17366 40-44
CTTGA 1556190 1.0598876 21.653528 90-99
CTCCG 2230250 1.0594325 9.988083 150-159
TACAG 1664005 1.0558465 9.304552 120-129
CTATA 1281830 1.05106 13.407664 50-59
GTAGC 1991020 1.0493538 11.6413765 50-59
GCTCT 1780055 1.0489805 17.958067 210-219
ATGGT 1596935 1.0441136 9.89227 35-39
CGTGA 1965830 1.0360774 20.967182 150-159
CAGCA 2017525 1.0319313 11.853389 45-49
ATGGG 2039090 1.0316824 19.222118 100-109
TAGTT 1219005 1.0299519 15.252221 50-59
ACCGC 2324510 1.0287269 11.381146 220-229
TTATA 1009280 1.0266514 14.533818 110-119
GAGCC 2414505 1.0257939 17.731892 220-229
GGGAG 2616125 1.0242766 14.951903 5
ATGAG 1681075 1.0239921 19.729399 60-69
ACCAA 1639205 1.0094038 17.786684 130-139
ACACC 1878675 1.000968 12.634726 40-44
ATGAT 1271005 1.0004783 9.86752 120-129
GACAG 2028800 0.99617225 15.120808 70-79
CGAGT 1888925 0.99554515 15.357458 160-169
AGCCG 2335350 0.9921652 18.78568 220-229
TTTCA 1121220 0.9868224 6.9684973 100-109
AAACT 1291745 0.9867855 16.763369 130-139
TGAGG 1947800 0.98549414 11.615635 220-229
GGTAT 1502870 0.9826118 9.637687 110-119
CGAGG 2398800 0.97833914 14.138342 220-229
CCAAC 1828115 0.9740293 14.747156 130-139
ATGAC 1527370 0.9691487 10.716334 160-169
GGGAC 2359995 0.96251273 10.275556 90-99
ACTCA 1452445 0.96002483 11.743572 190-199
TTTAG 1133000 0.95728534 29.169138 45-49
AGCCC 2161910 0.9567672 11.783046 80-89
CCGTC 2012100 0.955805 10.296399 140-149
GCAGT 1803635 0.95059365 6.057004 50-59
AACTC 1437705 0.95028216 15.781977 190-199
TATGG 1452125 0.94943357 12.86722 35-39
AAGGT 1551910 0.9453139 20.258886 15-19
CCGAG 2218320 0.94244546 17.6927 220-229
AATCA 1231800 0.94099253 14.484813 110-119
GCCAA 1831610 0.9368388 14.322262 180-189
CGGAC 2203300 0.93606424 11.043117 220-229
TCATA 1141375 0.9358913 9.17885 110-119
GCCAT 1695550 0.93087965 7.8476095 60-69
CGTAT 1366140 0.9304487 7.7623453 240-247
CCCAG 2093945 0.92668885 15.068366 80-89
CATAC 1401900 0.9266161 14.240357 130-139
CCCCA 2006360 0.92494124 17.279533 80-89
GATAC 1454755 0.92307293 22.946035 35-39
TGGTT 1312965 0.92143524 9.798413 50-59
CAGAA 1558590 0.9213549 17.569464 140-149
TTTAT 839965 0.9171146 10.06504 240-247
ACAGG 1851700 0.90921336 14.701485 70-79
CGGAG 2199495 0.89705354 73.62722 6
GTTAT 1061415 0.8968023 11.689297 110-119
AACTG 1413250 0.8967371 10.958249 60-69
TACAC 1351060 0.8930122 21.331997 35-39
GACTG 1693940 0.89277965 12.973589 90-99
ACGCT 1621355 0.8901456 15.696923 190-199
GAGAT 1455625 0.88666373 11.730811 210-219
TAACA 1153865 0.8814567 8.214109 120-129
TCCTT 1157425 0.8814095 11.082131 45-49
GGTAG 1729705 0.87514836 12.311879 120-129
TATTT 801425 0.8750347 10.55104 240-247
AAGTT 1102320 0.867697 16.75496 140-149
ATAGT 1098355 0.864576 15.996104 120-129
AGAAG 1500190 0.85134315 15.294954 140-149
ATATG 1079510 0.849742 5.8908563 50-59
GATGA 1385620 0.8440217 6.3445315 60-69
TGGCA 1600655 0.8436146 6.22966 100-109
TCTTC 1104995 0.8414828 48.20759 2
GTCTG 1486365 0.8408583 14.959146 3
GATCA 1319560 0.83728886 9.286008 110-119
CATTA 1011675 0.8295414 11.76585 110-119
AGTCT 1215445 0.82781357 15.626985 8
TTACC 1163310 0.82533324 9.773947 45-49
GTCTA 1211585 0.8251845 10.416798 30-34
ATCCA 1246295 0.8237656 9.5313425 180-189
CATGG 1557420 0.8208277 8.446647 200-209
GCCTA 1494270 0.82037425 16.036257 190-199
GGTTC 1449090 0.8197713 11.280455 160-169
CTATC 1148675 0.8149501 8.000175 110-119
TGATG 1237735 0.80926037 12.412712 120-129
GCTTG 1429205 0.80852205 16.543503 90-99
ATATA 847470 0.80312765 10.32159 130-139
ACAAA 1127095 0.80214965 12.722359 130-139
ACATC 1212080 0.80115044 5.796791 110-119
TCATC 1119265 0.7940846 10.204734 110-119
GGTGC 1807695 0.79135543 11.285095 60-69
CCTCC 1597325 0.790405 11.1484785 140-149
TATAC 953935 0.78219646 8.715573 130-139
TTATG 919240 0.7766769 10.906146 50-59
GGTTA 1164685 0.76149845 8.669466 50-59
CTGAC 1386315 0.7611055 9.672951 220-229
CGCCG 2061745 0.7578858 11.3402 220-229
CCAGC 1706940 0.7554173 6.1552067 80-89
CCCCC 1891090 0.75431776 13.743412 80-89
ATAAA 845025 0.74606854 8.654771 130-139
TATGA 941980 0.7414845 5.2855678 120-129
TGACT 1088030 0.7410339 5.0649724 2
GTTCA 1088020 0.7410271 13.6455965 160-169
GACCG 1731290 0.73553246 9.734991 210-219
GCGGA 1803255 0.73544896 9.3085985 220-229
TTGGA 1121890 0.73351806 6.432145 60-69
TCAAC 1100850 0.72763056 7.78037 110-119
TCCGG 1594435 0.7270929 12.25867 70-79
GGGAT 1433410 0.72523725 12.910034 100-109
ATCTT 822990 0.72434044 13.706983 3
GATTA 916975 0.7218017 5.8203735 50-59
AAATA 816235 0.72064996 13.215601 130-139
CATCC 1254350 0.717362 6.373042 110-119
ATGCC 1304625 0.71625656 8.1472225 50-59
AATAC 930420 0.71076334 11.838549 130-139
TGCCA 1285480 0.7057457 7.513464 60-69
AACGC 1375550 0.7035715 14.0583 180-189
ACGCC 1586565 0.70214456 12.567961 180-189
TTTAC 795335 0.70000035 13.294052 45-49
AATAA 792640 0.6998181 8.211216 130-139
CACAA 1135475 0.6992126 8.4511595 130-139
GACCA 1360675 0.6959632 6.088782 170-179
GGGCA 1700825 0.69367343 13.225857 150-159
GAAAT 944850 0.6929026 7.5241737 110-119
TATTG 814135 0.6878724 6.2655606 240-247
GGATA 1124745 0.6851151 14.949865 35-39
GCCGC 1854250 0.68161184 12.402326 220-229
TTTCT 720265 0.6804427 9.7735 4
CAACG 1325245 0.6778413 14.2975645 180-189
AGGCA 1379965 0.6775842 7.021478 80-89
CAAAC 1097400 0.67576647 13.571104 130-139
CACGC 1524350 0.6746109 12.748456 190-199
TATAG 848360 0.66779107 6.030011 120-129
CCCGT 1389310 0.65996194 10.122171 140-149
CAGTC 1195235 0.6562 5.270675 210-219
ATTAA 678215 0.6427286 6.4380198 110-119
TCAAT 783105 0.64212126 8.786972 110-119
CCGGC 1745805 0.6417481 9.949602 70-79
GACGA 1285450 0.6311759 9.715546 220-229
ACTTC 888595 0.63043123 30.64125 4
TAGCT 925045 0.6300283 12.839041 50-59
GCGAC 1482825 0.629973 7.0214653 70-79
AGTAA 852680 0.62531006 5.0879326 50-59
AGATG 1023690 0.62355953 5.362303 120-129
CCGTA 1124790 0.61752474 6.2867136 230-239
CACCG 1393850 0.6168573 9.294368 45-49
TCCAC 1076515 0.6156582 10.198472 180-189
TACTT 694810 0.611525 10.640967 1
AAGCA 1029385 0.6085172 6.06056 120-129
GGCCC 1639050 0.6025055 10.327536 70-79
TAATA 634890 0.6016705 8.890832 130-139
TGATT 710745 0.60051703 6.2955437 50-59
CACGA 1165125 0.59594256 8.717612 180-189
CCCTA 1034305 0.5915184 11.463241 110-119
AATGG 958760 0.5840088 6.48843 120-129
CTTTG 798665 0.58386564 7.19356 45-49
ACCCG 1315200 0.58205026 8.783971 140-149
ACGAC 1136070 0.5810814 9.128765 220-229
GGGGA 1480015 0.5794619 9.190053 4
GAAGA 1015460 0.57626367 6.211158 15-19
CGGCA 1346745 0.5721599 11.446241 70-79
GGTGA 1130195 0.57182485 6.0231 120-129
TCAGG 1076330 0.56727254 5.5093455 150-159
GTCCT 957530 0.56426924 6.7729454 45-49
TTAGC 824060 0.5612496 9.673361 45-49
ACCTA 848955 0.5611351 6.974717 130-139
ATTTC 636950 0.56060046 6.3674293 240-247
CTGCG 1219035 0.5559033 12.304487 25-29
CGGTT 974145 0.551088 11.178458 160-169
GCTAC 986135 0.5414013 8.014392 50-59
TTCAA 659220 0.5405395 5.284206 200-209
GTTCC 914990 0.5392006 6.25426 140-149
AAATC 705495 0.53893936 8.106971 110-119
ACCCC 1158155 0.53391486 9.456594 140-149
GGAGG 1357750 0.5315921 5.9134445 6
CCACG 1196890 0.5296914 7.6684756 180-189
AAGAT 721660 0.52922696 5.7832537 15-19
AAGTA 718630 0.52700484 5.3226547 120-129
TATGT 601980 0.50862014 6.301019 140-149
GGGTA 1001820 0.50687325 5.45176 100-109
CTGTT 690750 0.5049742 5.3973927 240-247
TAAAT 532750 0.5048748 8.818738 130-139
GGGTT 907280 0.49272215 5.6266985 100-109
TGACG 932140 0.49127817 8.69269 220-229
ACATT 597975 0.49032053 5.857205 110-119
CGCGG 1385580 0.48894927 6.6634927 220-229
GTAAC 749035 0.47527862 6.524917 120-129
TGGCC 1024820 0.46733758 15.008122 2
TATCC 656775 0.46596196 5.292622 110-119
CAATC 700635 0.4630998 6.6021886 110-119
AACAT 603170 0.4607716 5.5554 110-119
CCGGT 1009485 0.4603445 8.642594 160-169
CCGCG 1240560 0.45602286 6.9212456 220-229
ATGCT 664010 0.45224297 6.9775877 50-59
TGCTA 655520 0.44646057 6.185467 50-59
TGCGC 975980 0.4450656 9.146247 25-29
GGTTT 627095 0.44009355 5.0921445 100-109
ATTCT 495220 0.43585932 6.91923 2
GCGCT 943910 0.43044105 8.327314 25-29
GCCTT 730150 0.43027496 8.985098 1
CATAA 560900 0.42848083 5.6980395 110-119
GCATG 807505 0.42559007 8.422596 60-69
GGTCA 805625 0.42459923 5.3730927 160-169
ATGGC 786100 0.4143087 7.347956 1
CACTT 575805 0.4085162 7.081284 3
ACGAG 820840 0.40304515 7.657191 180-189
CATGC 728195 0.3997888 7.2737713 60-69
AGCTT 586280 0.3993027 7.2998857 1
CCGGG 1126225 0.397427 5.2997646 80-89
CTAAC 589040 0.3893387 5.1975794 110-119
TACCT 546755 0.3879061 5.15781 2
TTTGA 457480 0.38653037 6.102722 45-49
CCTAA 573580 0.37912008 7.2245007 110-119
GAGGC 912170 0.37202415 5.627127 35-39
GGTAA 606745 0.36958614 6.7656355 120-129
TTCTT 383155 0.36197093 19.69406 1
TGGGC 801995 0.35108975 8.789464 1
TGCTG 610200 0.34519902 13.6512165 1
CGAGC 805205 0.34208852 6.5396366 180-189
AACCT 469550 0.3103592 5.020756 2
CGGGA 744880 0.30379575 5.070682 80-89
AACTT 366970 0.30090377 10.797123 3
CTCTT 394585 0.30048683 9.453618 1
CGCTT 505985 0.29817528 6.1427917 1
GCGAG 719225 0.2933325 5.1806645 240-247
GACTT 423665 0.28854913 7.0549006 1
TCACA 433725 0.2866799 5.0805297 5
TTCCT 366055 0.27876052 6.233273 4
ATTGT 328395 0.2774649 5.107162 240-247
GAGGT 539320 0.27287024 7.50174 7
TAACT 323655 0.26538682 7.1956882 2
CCCTT 429005 0.26334983 5.8555775 3
GTCTT 359075 0.2625025 12.559766 1
TTGGC 454945 0.257369 7.9031887 1
TCGGT 453705 0.25666752 5.6459246 7
TGTAA 322830 0.25411734 7.6499476 25-29
AATCT 307905 0.25247234 7.788155 2
CGGGG 744225 0.25211564 9.221477 3
CCTCG 491815 0.23362619 5.3369927 5
TGCTT 296410 0.21669112 10.535601 1
TGCCT 333100 0.19629472 5.0193267 4
TCGCA 308890 0.16958475 7.1555676 7
TAGGC 298940 0.15755431 6.8821535 1
TTGCT 176260 0.12885523 6.1770644 4
Produced by FastQC (version 0.10.1)