FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_6_SL248352.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_6_SL248352.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11106874
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 12949245 8.835317 63.08808 110-119
ATACA 11791300 7.6307626 38.874763 110-119
CACAG 17198050 7.5596385 43.111565 110-119
TCCCT 20319875 7.1531043 110.0368 30-34
CAGTA 14721170 6.8223743 40.952305 60-69
AGTAA 11193990 6.468142 49.036037 110-119
GGAGA 17864775 6.2603135 96.18879 35-39
CCCTG 20410070 6.0846415 72.23346 30-34
GTAAT 12227375 5.9744496 40.725136 110-119
GGTGA 19828090 5.8755627 102.68462 45-49
AATAC 8553735 5.535567 41.01633 110-119
GTAGT 15092585 5.280989 26.314657 80-89
ACAGT 11318720 5.245544 36.43131 110-119
AGACG 13283305 5.2133436 102.62496 40-44
GTGAC 15189315 5.0410194 85.31822 45-49
ACTCC 11530020 4.7999153 124.7472 30-34
GAGAC 12144090 4.7662315 142.21762 40-44
CCCCA 12061060 4.7623086 102.45674 60-69
ACCAG 10685290 4.6968656 97.86934 45-49
CTCAG 12568595 4.67174 35.421085 140-149
TAGTA 9137075 4.46449 33.48425 80-89
CACTC 10723725 4.464257 102.44583 25-29
GCACA 10013055 4.4013753 34.901253 110-119
GGCCC 17375555 4.386782 60.004234 60-69
CGTGT 15430255 4.3303533 23.736622 120-129
GTGTC 15408525 4.3242545 24.151226 120-129
TGACC 11576685 4.303047 145.83966 45-49
CTCCC 12788175 4.269831 71.376785 30-34
AGTAG 10218510 4.2283325 28.778257 70-79
ATGCA 8937200 4.141853 99.31575 25-29
GACCA 9301165 4.0884547 109.792534 45-49
CCCAG 11343695 3.9992168 87.443245 60-69
CCACT 9242340 3.8475604 28.534113 240-247
TGAGG 12920270 3.8286011 70.02588 35-39
CAGGG 13395985 3.765059 37.051598 50-59
TACAC 7242800 3.7593381 28.481651 110-119
AGGAG 10720630 3.7568061 96.66622 35-39
ATGGT 10603760 3.7103214 25.877321 190-199
ACGGT 11107205 3.6862516 97.21 40-44
CTGAG 11060930 3.6708944 106.712685 35-39
GACGG 13052270 3.668455 108.88904 40-44
AGATA 6336730 3.6615067 19.803373 150-159
TGCAC 9759565 3.6276245 119.81769 25-29
GAGGA 10138225 3.5527158 126.7452 35-39
GGGTT 14104960 3.5343573 43.81488 50-59
TGTCC 11219380 3.526393 20.86069 120-129
CCTGA 9341360 3.4721785 87.71717 30-34
CCCTT 9826165 3.459056 32.55643 20-24
GCCCC 12138000 3.4321492 73.44758 60-69
CCAGG 10760455 3.3871887 42.072636 50-59
TGGTG 13485660 3.3791754 17.997208 190-199
TCTTG 10175345 3.3719604 18.475258 170-179
GCACT 9071420 3.3718414 92.783615 25-29
GGATG 11348565 3.3628654 72.31686 20-24
CTTCA 7488130 3.2866113 17.03743 200-209
CTCTC 9296625 3.272645 13.759714 100-109
CCGTG 12272725 3.2667794 23.868301 120-129
CAGAT 6984395 3.2368457 17.984827 150-159
TCAGG 9741485 3.2329977 21.16792 140-149
CTTGG 11472950 3.2197735 18.29818 20-24
AGGCT 9698910 3.2188678 22.819138 30-34
CCTGG 12068845 3.2125103 30.435736 50-59
TCGCA 8565840 3.183918 24.822935 100-109
TGTCT 9511275 3.1518967 19.890057 180-189
TTCCC 8840750 3.1121652 51.776512 50-59
CGGAT 9372610 3.1105754 82.2744 20-24
TCTCA 7083490 3.109011 21.976059 130-139
CGCAC 8798665 3.1019676 25.484024 100-109
GGTTG 12312735 3.0852695 75.3082 15-19
ACAGA 5558795 3.0465186 14.827157 140-149
ACCAC 6130405 3.0180237 20.394907 230-239
GGTTC 10712265 3.0062947 49.523678 50-59
GATGC 9047360 3.0026317 99.0664 25-29
AGGGT 10035565 2.9737904 52.03891 50-59
TGGCC 11051775 2.941784 44.09503 60-69
GTTGG 11491915 2.8795922 54.130962 15-19
GACTC 7727505 2.872309 20.21039 190-199
TCAAA 4411830 2.8551247 59.47204 70-79
TCTCT 7608530 2.8238769 15.040181 180-189
AGTCA 6034880 2.7968032 33.225502 70-79
TTCTT 7133045 2.7912023 21.001123 170-179
GCCCT 9354285 2.788695 17.734335 20-24
CCAGT 7496875 2.7865841 37.807606 60-69
CGGTG 11577955 2.751687 68.33547 40-44
CGGCC 10829505 2.73411 13.100114 120-129
GCCGT 10248385 2.7279365 22.543547 120-129
GTTCC 8676045 2.7269907 56.124653 50-59
GATGG 9063940 2.6858735 18.33349 15-19
TCACA 5132060 2.6637695 11.938515 140-149
AGATG 6412805 2.6535642 17.158749 180-189
GCGGA 9196480 2.5847514 90.333244 20-24
AGGGA 7369110 2.582341 9.575702 150-159
TCCAT 5696465 2.5002325 16.324074 70-79
TGTTC 7515130 2.490404 13.038408 140-149
TACCA 4765605 2.473563 16.975761 230-239
TCCTC 7009700 2.46759 17.048067 130-139
GGCGG 12254150 2.4664216 43.331867 20-24
ACAGC 5609895 2.4659061 11.168735 120-129
TGGAG 8277890 2.4529471 15.452114 25-29
GGCTG 10275015 2.4420223 11.790341 140-149
ACTCT 5521935 2.4236295 13.620218 200-209
TTGGG 9614500 2.409158 81.80617 15-19
GTTCT 7248980 2.4022055 18.309738 160-169
GAGAT 5794220 2.397599 16.705273 180-189
CTGGC 8981660 2.3907568 36.0054 60-69
CAGCT 6376050 2.3699741 12.558637 150-159
CACGG 7461450 2.3487241 9.955254 120-129
AATAA 2886055 2.3286934 9.738434 110-119
CTGGA 6994510 2.3213334 14.599618 180-189
ACTAC 4433085 2.3009698 13.150744 230-239
TGTAG 6550635 2.2921078 8.391766 210-219
CATTT 4922995 2.2781155 17.828564 150-159
TCATT 4916270 2.2750037 16.652164 150-159
CTACT 5182335 2.274576 7.829039 180-189
CACTA 4344525 2.2550035 15.73739 240-247
TTCAT 4861275 2.2495544 16.341545 140-149
CAGAG 5706640 2.2397041 9.786401 140-149
GTGAA 5368810 2.221568 18.06928 190-199
GATAC 4766170 2.2088325 14.9187355 150-159
TTCAC 5029575 2.2075279 18.71305 200-209
TCAGC 5906635 2.1954927 15.054004 140-149
TGGGG 10132385 2.1501422 54.145382 15-19
TAGTC 5474650 2.145456 27.56582 70-79
TTGTC 6431340 2.1312518 13.307761 170-179
ATTTG 5146390 2.126363 15.589594 150-159
ATATA 3092735 2.1101844 10.980391 110-119
TTGGA 6005260 2.1012778 16.21413 170-179
CTCGC 7009530 2.089678 16.743967 100-109
AATAT 3059125 2.0872524 13.612377 110-119
TCTGC 6595135 2.0729344 12.000049 210-219
GTCAA 4447480 2.0611386 42.234535 70-79
TACAG 4444410 2.059716 14.134148 160-169
AGCTC 5530825 2.0558045 12.909819 140-149
ACACA 3339820 2.050019 14.132596 110-119
GAATC 4417500 2.047245 14.243036 190-199
CTCTG 6490490 2.0400434 14.524338 180-189
GAACT 4398270 2.0383332 11.298757 160-169
GTCTC 6477300 2.0358973 11.94885 180-189
GCTGT 7238900 2.031528 10.180251 140-149
GTCCT 6441985 2.0247972 14.328236 130-139
ATGTA 4138980 2.0223577 7.337354 150-159
GTTCA 5147145 2.0171103 15.254309 140-149
TCTCG 6405965 2.0134761 16.11786 100-109
TGAAT 4104675 2.005596 13.761619 190-199
CAGGC 6364190 2.0033271 15.455788 140-149
AACCA 3252990 1.9967221 15.784192 70-79
GATAT 4074345 1.9907764 8.653714 180-189
AATCG 4269795 1.9787927 13.308028 190-199
ATCTC 4498935 1.974625 7.983132 100-109
ACGGC 6257290 1.9696771 14.892747 120-129
TTGCA 5024150 1.96891 14.355328 150-159
TACTA 3589325 1.9642153 10.252081 230-239
CCTTC 5552545 1.954635 14.029614 200-209
ACACG 4436805 1.9502584 13.84354 120-129
ATATG 3988285 1.9487262 8.96468 180-189
TGGAA 4671385 1.9329795 9.482157 170-179
GAGTC 5806785 1.9271519 12.335084 210-219
GGGGC 9504585 1.91301 49.437115 15-19
TTTGC 5766575 1.9109584 12.163889 150-159
CAGCC 5407095 1.9062703 10.191233 120-129
CATAG 4090840 1.8958576 8.603463 210-219
TCGGC 7119810 1.8951658 8.065959 130-139
AGTAT 3808650 1.8609544 12.230198 220-229
GGGCG 9245445 1.8608522 60.56386 20-24
AGAAC 3287285 1.8016092 11.834159 160-169
CTGTT 5416580 1.7949752 12.806263 140-149
GTTGT 6055500 1.7917262 7.2522793 210-219
GACGT 5342445 1.773047 10.091096 170-179
CGTAG 5295870 1.7575897 9.773547 210-219
GCAGA 4457745 1.7495462 14.677369 150-159
AGTCT 4413310 1.7295285 13.15372 210-219
CACCA 3500455 1.7232884 13.587667 230-239
ATTGT 4144575 1.7124374 7.6560497 170-179
GTCAC 4600255 1.7099121 9.068617 130-139
TGCAG 5137840 1.7051429 13.53393 150-159
CTCCA 4089955 1.7026368 7.857235 150-159
CCAGA 3870115 1.7011621 25.427402 60-69
ATAGT 3457535 1.6893951 7.3001432 220-229
AACTG 3644340 1.6889321 11.154308 160-169
GGTCA 5088240 1.6886816 7.9721026 130-139
TCCCA 4053855 1.6876085 10.782624 240-247
TGGTT 5658645 1.6743032 18.44611 10-14
GAGAA 3419995 1.6735436 10.851105 160-169
TGACT 4266955 1.6721735 11.980853 190-199
ACTGA 3525155 1.6336967 9.163622 180-189
AATAG 2814655 1.6263717 10.027126 240-247
TCAGA 3508925 1.6261753 10.419095 130-139
AAAAA 1702080 1.6241182 8.5045185 8
CAAAA 2118550 1.6213433 20.97179 70-79
CAAAG 2949985 1.6167505 30.558361 70-79
TAGTT 3898145 1.6106185 10.093354 220-229
TTGTA 3896605 1.6099823 9.385787 210-219
TTCAG 4097435 1.6057404 9.686657 150-159
GCTCC 5386145 1.6057152 6.28632 150-159
GAACC 3643835 1.6016977 12.973999 70-79
GTCTG 5704255 1.6008445 9.0655 210-219
GTGCT 5698180 1.5991396 8.482151 220-229
ATAGA 2753970 1.5913063 10.425138 240-247
TCTGG 5668055 1.5906854 11.890225 180-189
TGCTC 5059205 1.590172 5.174699 150-159
TGTGC 5653425 1.5865797 8.119356 220-229
CTGGT 5642810 1.5836008 7.2892737 160-169
CCTTG 5008335 1.5741833 20.851877 20-24
GCTCA 4208265 1.5642096 11.648828 140-149
CCCAC 3956940 1.5623975 10.81459 240-247
GGCCG 6912845 1.5583054 13.456095 120-129
CCACC 3940055 1.5557305 10.039879 230-239
GGACG 5520580 1.5516075 7.532088 170-179
CTGTG 5527075 1.5511208 5.3333144 210-219
TTGGT 5202565 1.5393564 22.271368 25-29
GTCCC 5093455 1.5184585 18.82425 50-59
ACCAT 2877960 1.4937903 11.01259 45-49
GCTCT 4731135 1.4870555 10.198807 130-139
CCTCG 4922220 1.4674101 9.714164 130-139
TATAC 2672550 1.4625211 6.97401 110-119
CGACT 3905340 1.4516126 9.070024 190-199
TGATA 2965590 1.4490246 8.177068 180-189
CACTG 3858630 1.4342505 7.4449615 230-239
AATTG 2903155 1.4185182 9.541528 170-179
TCGAC 3814100 1.4176989 9.753117 190-199
TGGAC 4254625 1.4120221 10.598915 170-179
CTCTT 3790470 1.4068184 8.402191 200-209
GGGAG 5577750 1.3997309 6.8775434 150-159
GAGGG 5544095 1.3912852 8.609946 200-209
CATGT 3526005 1.3818032 7.043091 70-79
GCTGA 4135965 1.3726412 19.419882 30-34
CAGAC 3121415 1.3720608 14.836772 60-69
GCGTA 4107980 1.3633536 10.254299 210-219
CCATC 3242090 1.349673 10.771892 240-247
ACGGA 3435435 1.3483168 9.524095 200-209
CTGAT 3430330 1.3443093 7.5999017 180-189
ACTTC 3040325 1.3344274 6.616123 210-219
CCTCA 3200595 1.3323988 7.3608027 130-139
CTGCT 4238425 1.3321906 6.03794 90-99
CTCGA 3579635 1.3305484 8.732678 190-199
GAATT 2712855 1.3255352 10.548922 170-179
TATGG 3773485 1.3203658 6.3743935 180-189
CTTGA 3350595 1.313062 8.638971 200-209
TCCAC 3150595 1.311584 9.90476 240-247
GTAGG 4394230 1.3021209 5.0566673 160-169
ACGTG 3864460 1.2825344 9.713398 170-179
AAAGT 2214975 1.279863 22.534315 70-79
AGATC 2760840 1.279483 7.1311765 130-139
ACTAT 2335465 1.2780553 6.309316 230-239
AGCCG 4040820 1.2719741 9.008569 120-129
AAGTA 2200325 1.2713978 16.688625 70-79
GTGTT 4288565 1.2689183 7.5780406 160-169
GGAGT 4276280 1.2671694 7.118738 210-219
GTGGA 4240180 1.256472 19.633036 25-29
CCATG 3366455 1.2513094 7.132431 70-79
ACTCG 3365640 1.2510064 8.98493 190-199
GGCTC 4696315 1.2500747 8.520184 130-139
ATAAC 1927960 1.247683 7.8293715 80-89
ATCGG 3753585 1.2457372 7.796738 190-199
GGAAT 2996580 1.2399594 8.794531 170-179
ACCGT 3312720 1.2313361 17.674057 45-49
ACTGG 3707965 1.2305969 7.5050592 160-169
GGAGG 4896775 1.2288408 14.929669 30-34
TTGAG 3469635 1.2140468 8.361286 200-209
ATAAA 1502415 1.212265 5.7035766 7
TCACG 3252595 1.2089878 9.65957 200-209
AGCTT 3078205 1.2063153 6.44898 150-159
GATCT 3066660 1.2017909 5.614647 130-139
GCATA 2572640 1.1922635 8.214607 210-219
TTCCA 2716200 1.1921659 7.4650545 230-239
CAATA 1831900 1.1855177 6.618452 100-109
GTCCA 3187455 1.1847751 15.291295 70-79
GTGGT 4712550 1.1808494 9.905857 50-59
TTGGC 4191065 1.1761823 7.649715 60-69
ATACC 2239845 1.16258 7.151482 70-79
AGAGC 2953460 1.1591544 7.482687 140-149
ACTGT 2947205 1.1549777 5.7374024 230-239
TGGTC 4088130 1.1472945 11.219105 50-59
TCTAC 2608420 1.1448603 7.574473 180-189
TGCCC 3798865 1.1325159 6.21374 60-69
GTCTA 2880770 1.1289426 7.5832534 180-189
ATCCA 2170740 1.1267115 15.915591 240-247
TATCA 2057570 1.1259806 6.5599623 70-79
AGTTC 2861865 1.121534 5.492676 160-169
AGGTT 3185775 1.1147225 23.689531 10-14
TACTG 2823310 1.1064246 6.813445 180-189
GCTTC 3517960 1.1057392 5.81208 150-159
CTACC 2652235 1.1041181 8.970052 230-239
CCATA 2118380 1.0995343 9.922883 70-79
TATGT 2661055 1.099483 7.8026032 220-229
TCTGT 3312430 1.0976907 6.4226866 210-219
CGGCT 4120175 1.0967168 7.969027 130-139
ATATC 1998730 1.0937811 7.3728876 70-79
GAGCT 3286295 1.0906533 6.7069683 140-149
CTCGG 4079055 1.0857713 8.464372 130-139
AAAAG 1585525 1.0834222 13.864954 70-79
AGGGG 4256170 1.0680817 5.0656877 60-69
CTGCG 3976610 1.0585023 6.96655 210-219
GGGGT 4942880 1.0489036 8.099849 10-14
TAGAT 2132870 1.0421472 8.0453205 240-247
ATACG 2245100 1.0404685 5.4821854 120-129
AACTT 1886425 1.0323236 6.087151 200-209
CTGCC 3455150 1.0300479 5.6989765 200-209
GTCCG 3857750 1.0268638 5.066339 120-129
GAAGA 2092870 1.024127 16.707775 35-39
ACCCA 2075140 1.0216 13.587741 240-247
GCGGT 4281355 1.0175328 5.96151 130-139
AGAGA 2073060 1.0144331 12.230326 35-39
CATGG 3048795 1.011832 5.269145 190-199
TATAA 1478175 1.0085642 6.376342 240-247
TGCGT 3577635 1.004029 8.260657 210-219
CTGAA 2143470 0.9933693 13.6365 35-39
AAAAT 1229655 0.9921812 5.050309 8
ATGGG 3329675 0.9866665 28.52013 15-19
CAGCG 3119195 0.98186386 6.449767 160-169
CTTCT 2644980 0.9816742 5.482788 210-219
CGAAC 2230585 0.9804842 13.651128 70-79
ACTAA 1503475 0.97297674 12.942256 240-247
AGAAA 1419840 0.97020614 5.1918626 7
TGTAA 1975425 0.96521753 7.0184298 230-239
CGGAG 3426675 0.9630971 6.668451 210-219
CCAAT 1845750 0.958027 11.120481 240-247
AGCTG 2877980 0.95514214 5.159452 90-99
TCCCC 2847485 0.950744 5.1388273 80-89
CCCAA 1930315 0.950302 10.382721 240-247
ACAAT 1439255 0.93141675 7.6678543 100-109
TAGGC 2784330 0.92406154 5.7841215 160-169
GAGTT 2639060 0.92342347 5.223393 160-169
TCGAA 1961995 0.90926653 17.16316 70-79
CCATT 2063755 0.9058016 5.4393554 50-59
CTATA 1647720 0.9016952 5.0316014 90-99
TGCAT 2288760 0.89694023 6.2037854 210-219
CGTGG 3743675 0.88974446 6.7967477 50-59
AAGAG 1788340 0.875108 12.198773 35-39
CCAGC 2465400 0.86917627 5.8843937 80-89
TTCTG 2600870 0.8618902 5.4135513 210-219
CATCA 1655075 0.8590582 5.4594364 230-239
TGAAG 2068090 0.8557581 8.991565 35-39
TGAAC 1843660 0.8544253 6.7535295 190-199
CGGTC 3209630 0.8543459 5.801115 130-139
CTTCC 2412545 0.8492763 5.294974 230-239
AGCGT 2554055 0.8476381 6.684037 160-169
GTATG 2393795 0.83760375 5.410603 220-229
GGTGG 3924655 0.83283126 19.647327 25-29
ACGTC 2182785 0.8113399 10.386742 70-79
ATCAA 1250375 0.8091826 12.358315 70-79
AACCG 1831970 0.8052676 5.9761515 190-199
GAGGC 2859015 0.8035512 12.704412 30-34
TAACC 1546035 0.8024615 6.2493196 80-89
GGTGC 3272620 0.7777907 5.0053625 220-229
CTAAT 1419600 0.7768592 9.990837 240-247
AAAGG 1568175 0.76737225 8.97338 10-14
TGAGA 1849635 0.7653633 5.862192 240-247
GGGCC 3377440 0.7613483 14.861664 15-19
AATCC 1455500 0.75546986 9.873628 240-247
AAAGA 1101680 0.7528008 5.8698363 8
AGCCA 1701475 0.7479066 6.0444922 80-89
ATGAT 1520580 0.7429746 6.844945 240-247
CGTCC 2471810 0.73689485 8.489295 70-79
GTCGA 2194915 0.7284469 12.236368 70-79
TCCAG 1934300 0.7189782 5.0836897 240-247
GTAGC 2155125 0.71524143 5.6465487 80-89
GGTCC 2679405 0.71320957 11.473992 50-59
GACCG 2252105 0.70892024 16.393835 45-49
AAGGT 1709750 0.7074801 10.006737 10-14
TGGGC 2971105 0.70613074 16.124985 15-19
CATAC 1329630 0.6901376 8.502636 70-79
CAATC 1285385 0.6671725 9.640138 240-247
TAGGT 1906350 0.6670437 5.603336 10-14
AAAGC 1207165 0.6615914 9.518569 70-79
TATGA 1353790 0.6614788 5.589129 240-247
AGGAT 1545485 0.6395086 5.4534984 10-14
GAGGT 2127265 0.63036215 6.9690533 10-14
GGAAG 1793340 0.6284361 5.526252 30-34
AGAAG 1272890 0.62287724 5.048257 9
AGACC 1415510 0.62220687 5.249624 240-247
ATAGG 1491130 0.6170169 5.3091617 10-14
AATGG 1445230 0.598024 6.827658 10-14
CCGTA 1592610 0.5919723 5.0600605 80-89
GGGTC 2431120 0.57779473 5.8425937 70-79
CATCC 1385890 0.57694215 7.3836327 240-247
CGATG 1719480 0.5706599 12.533628 15-19
TAAAA 706750 0.57026076 6.0436163 8
AAATG 964280 0.5571829 5.722688 9
AAGAA 801290 0.5475381 5.3115554 9
GAAGG 1516770 0.53151834 5.5438194 10-14
AAGGG 1501875 0.5262987 6.457113 10-14
GAAAA 747315 0.5106559 6.151926 8
TGATC 1273490 0.49906695 5.367203 240-247
GGTCG 2091705 0.4971273 5.936272 70-79
AAGCA 905005 0.49599147 6.2768416 70-79
GATCC 1300400 0.4833579 5.418562 240-247
ATAAG 807550 0.4666207 5.7062716 9
TAAGG 1069365 0.44249412 5.8897777 10-14
ACGAT 953535 0.44190595 5.808448 10-14
CGGTT 1565145 0.43924296 8.235349 10-14
TAAAG 716975 0.41428444 5.7519503 9
TGGGT 1557820 0.3903515 5.043385 10-14
AAGCT 833630 0.38633728 7.408049 30-34
Produced by FastQC (version 0.10.1)