FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_9_SL248355.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_9_SL248355.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12738936
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 14571055 8.501457 59.756756 110-119
ATACA 13334345 7.369802 36.81495 110-119
CACAG 19401380 7.355062 41.562283 110-119
TCCCT 22776055 7.030761 106.28148 30-34
CAGTA 16395875 6.5615516 38.65583 110-119
AGTAA 12637030 6.2528286 46.747826 110-119
GGAGA 20112610 6.111064 93.68091 35-39
CCCTG 22733535 5.9514103 69.63948 30-34
GTAAT 13804485 5.8319054 39.006527 110-119
GGTGA 22458615 5.8262625 101.18618 45-49
AATAC 9693645 5.3576117 38.73924 110-119
GTAGT 17161115 5.249568 28.738043 80-89
AGACG 15334165 5.2042866 101.12456 40-44
ACAGT 12793070 5.1197267 35.075333 110-119
GTGAC 17213620 4.9880643 83.853714 45-49
GAGAC 14008835 4.754481 140.63986 40-44
CCCCA 13795455 4.7247944 100.951866 60-69
ACTCC 12921265 4.671648 120.43068 30-34
CTCAG 14332230 4.6390195 34.505074 140-149
TAGTA 10674875 4.5097556 36.38037 80-89
ACCAG 11867230 4.498866 92.24881 45-49
GGCCC 19887370 4.415291 59.268177 60-69
GCACA 11467155 4.347198 33.75582 110-119
CACTC 11893105 4.2999196 97.98668 25-29
GTGTC 17371625 4.297926 23.591557 120-129
CGTGT 17219375 4.2602577 23.055796 120-129
TGACC 13148030 4.255721 143.35532 45-49
AGTAG 11677100 4.18364 29.577692 80-89
CTCCC 14194495 4.15074 69.38154 30-34
ATGCA 9941430 3.9785132 95.30979 25-29
CCCAG 12945985 3.9694405 86.408325 60-69
GACCA 10398270 3.941984 104.72792 45-49
TGAGG 14505005 3.7629201 68.99309 35-39
AGGAG 12177475 3.7000334 93.94866 35-39
CCACT 10138800 3.6656556 26.813843 240-247
ATGGT 11949300 3.6552787 25.299913 190-199
TACAC 8166730 3.650672 26.936249 110-119
GACGG 14848555 3.6489933 108.10048 40-44
ACGGT 12581675 3.6458452 96.105156 40-44
CAGGG 14777020 3.6314137 35.477 50-59
CTGAG 12484020 3.617547 104.83863 35-39
CCCTT 11658660 3.5989223 35.57884 20-24
TGTCC 12907155 3.5669887 20.517492 120-129
AGATA 7189390 3.5573251 18.979923 150-159
TGCAC 10908420 3.5308096 115.18479 25-29
GAGGA 11463530 3.4831066 123.38875 35-39
GCCCC 13859640 3.437056 73.11036 60-69
GGGTT 15441980 3.4203362 42.175312 50-59
TGGTG 15410140 3.4132838 17.76778 190-199
CCTGA 10380285 3.3598642 83.85829 30-34
CAGAT 8336980 3.33642 17.689768 150-159
GGATG 12817565 3.3251605 69.81353 20-24
CCGTG 14071990 3.2980392 23.203085 120-129
AGGCT 11369785 3.2946706 24.810463 30-34
CTTGG 13261180 3.280958 20.325592 20-24
CCAGG 11935590 3.2763135 40.160988 50-59
TCTTG 11198790 3.267085 17.734032 170-179
GCACT 10054915 3.2545493 89.01802 25-29
CTCTC 10519405 3.2472446 13.554804 100-109
CTTCA 8475335 3.234746 16.537518 200-209
TCAGG 11027720 3.195549 20.643665 140-149
CCTGG 13536385 3.17251 29.385649 50-59
TCGCA 9624470 3.1152236 23.832687 100-109
TCTCA 8119195 3.0988197 21.630234 130-139
TGTCT 10582945 3.087422 19.368004 180-189
CGCAC 9891155 3.0327826 24.390686 100-109
GGTTG 13632810 3.019612 74.03022 15-19
CGGAT 10407000 3.0156803 79.167946 20-24
TTCCC 9705390 2.9959655 49.521294 50-59
ACAGA 6367760 2.9846947 13.813759 140-149
TGGCC 12478730 2.9246283 43.578125 60-69
GATGC 10046485 2.9112124 96.13977 25-29
GGTTC 11728510 2.9017587 47.57237 50-59
AGGGT 11088795 2.8766792 49.642925 50-59
ACCAC 6786015 2.873566 19.238329 230-239
GCCCT 10949960 2.866589 19.19604 20-24
GTTGG 12733385 2.8203938 53.170517 15-19
AAAAA 3513325 2.811922 14.017329 130-139
TCTCT 8615690 2.8075798 14.989375 180-189
GACTC 8637770 2.7958515 19.020891 190-199
CGGTG 13206230 2.7709358 68.335655 40-44
TCAAA 5002495 2.7648451 56.895405 70-79
GATGG 10621545 2.7554643 19.761412 15-19
GCCGT 11678695 2.7371247 21.964458 120-129
AGTCA 6801990 2.7221243 31.267786 70-79
CCAGT 8347680 2.7019558 36.16558 60-69
CGGCC 12160025 2.699706 12.569781 120-129
TTCTT 7829220 2.693263 20.048794 170-179
GTTCC 9581910 2.6480324 53.87414 50-59
AGATG 7314700 2.620691 16.931028 180-189
TCACA 5806050 2.5954065 11.143565 140-149
TCCAT 6763210 2.5812864 17.921692 70-79
GGCTG 12058090 2.5300326 13.016644 30-34
GCGGA 10220180 2.5115824 87.23855 20-24
TGGAG 9609755 2.4929838 16.963217 25-29
TCCTC 8060665 2.4882538 16.86322 130-139
AGGGA 8144215 2.4745579 8.825181 150-159
TGTTC 8447160 2.4643373 12.729676 140-149
GGCGG 13671890 2.4327931 42.600235 20-24
ACAGC 6347070 2.406174 11.048415 120-129
ACTCT 6275365 2.3950927 13.123947 200-209
GAGAT 6652040 2.3832748 16.396557 180-189
TTGGG 10677970 2.3651276 79.786575 15-19
TACCA 5280250 2.3603647 16.046312 230-239
GTTCT 7998280 2.333383 17.34917 160-169
CTGGC 9943395 2.330424 34.7833 60-69
AATAA 3408600 2.3292694 9.846746 110-119
CTGGA 8002070 2.3187938 14.428488 180-189
CAGCT 7143345 2.3121395 12.026442 150-159
CACGG 8385510 2.3018181 9.478707 120-129
TGTAG 7468375 2.2845683 7.7713447 210-219
CATTT 5569085 2.2438066 17.472994 150-159
TCATT 5542080 2.2329261 16.227024 150-159
TTCAT 5457535 2.1988628 15.808084 140-149
CAGAG 6466260 2.1945941 9.145876 140-149
TTCAC 5749440 2.1943653 18.120842 200-209
GTGAA 6120130 2.1927037 17.676989 190-199
TCAGC 6772825 2.192211 14.300558 140-149
ACTAC 4871210 2.1775165 12.096659 230-239
TTGTC 7411805 2.1622875 13.290437 170-179
CTACT 5649465 2.156208 7.2876306 230-239
CACTA 4774705 2.134377 14.72298 240-247
GATAC 5313090 2.1262734 14.372566 150-159
TGGGG 11227650 2.109035 52.70843 15-19
TCTGC 7629205 2.1083877 12.03662 210-219
ATTTG 5842325 2.1073399 15.439053 150-159
CTCGC 7934150 2.0770805 16.34385 100-109
GCTGT 8340495 2.0635278 10.052481 140-149
CTCTG 7456235 2.060586 14.481043 180-189
TAGTC 6012850 2.0545232 25.988886 70-79
GAATC 5124035 2.0506146 14.225764 190-199
ATGTA 4840960 2.0451336 7.8249207 70-79
TCTCG 7399925 2.0450244 16.272905 100-109
GTCCT 7383750 2.0405543 14.209978 130-139
TTGGA 6668035 2.0397453 15.316471 170-179
ACACA 3894550 2.0390282 13.538069 110-119
GTCTC 7376560 2.0385673 11.8006525 180-189
GAACT 5079710 2.032876 10.419108 160-169
ATCTC 5323975 2.0319796 8.310446 100-109
ATATA 3468265 2.0235534 10.318532 110-119
TACAG 5042895 2.0181427 13.433605 160-169
AGCTC 6206290 2.0088363 12.195663 140-149
CAGGC 7317195 2.0085664 15.325689 140-149
TGAAT 4741010 2.0029085 13.702872 190-199
GTTCA 5838015 1.9947841 14.787482 140-149
AATCG 4927205 1.9718441 13.198651 190-199
GATAT 4665750 1.9711136 7.956497 180-189
GTCAA 4917270 1.9678683 39.95159 70-79
AATAT 3360660 1.9607713 12.939361 110-119
TTGCA 5735110 1.9596226 14.133835 150-159
ACACG 5150300 1.9524784 13.076514 120-129
TTTGC 6677915 1.9481856 12.083897 150-159
CCTTC 6294165 1.9429514 13.776363 200-209
TGGAA 5398275 1.9340794 9.052539 170-179
AACCA 3688755 1.9312822 14.642513 70-79
ACGGC 7020795 1.9272046 14.188077 120-129
GAGTC 6613830 1.9165175 12.151847 210-219
TCGGC 8059295 1.8888493 7.7820115 130-139
TACTA 3992010 1.8837987 9.79028 230-239
CAGCC 6133000 1.8804733 10.103445 120-129
GGGGC 10567200 1.8803407 48.135094 15-19
ATATG 4432965 1.8727702 8.092298 180-189
CATAG 4669685 1.8687859 8.2398815 210-219
GGGCG 10267890 1.8270812 59.59378 20-24
CCAGA 4799210 1.8193804 27.13653 60-69
AGTAT 4263710 1.801266 11.568242 220-229
CTGTT 6105905 1.7813096 12.482125 140-149
GACGT 6136345 1.7781546 9.359458 170-179
GTTGT 6789500 1.7732687 6.8035107 210-219
CGTAG 6089490 1.7645773 9.64915 210-219
TCAGA 4409090 1.7644969 10.229877 130-139
CTCCA 4868820 1.7603086 7.9995937 150-159
GCAGA 5075630 1.7226261 14.200684 150-159
ATTGT 4771000 1.7209107 7.6428037 50-59
CACCA 4048235 1.7142417 13.217493 230-239
TGCAG 5906780 1.7116326 13.34127 150-159
CCTTG 6192520 1.711349 23.088959 20-24
AGAAC 3644640 1.7083147 10.900919 160-169
GTCAC 5257500 1.7017343 8.344463 130-139
AGTCT 4976115 1.7002825 12.844389 210-219
ATAGT 3959645 1.6728094 7.326621 220-229
GGTCA 5734150 1.6616089 7.358103 130-139
TGACT 4840955 1.6541 11.4838085 190-199
CAAAA 2532700 1.6394917 20.995777 70-79
TCCCA 4525980 1.6363558 10.455967 240-247
TGGTT 6249940 1.6323473 18.058336 10-14
CTGTG 6547170 1.6198399 5.4134707 210-219
TGTGC 6546440 1.6196595 8.078729 220-229
GTCTG 6539315 1.6178966 8.969107 210-219
AACTG 4029760 1.6126909 10.313591 160-169
TTCAG 4716940 1.6117254 9.026478 150-159
GTCCC 6136135 1.6063782 20.613295 50-59
GCTCC 6111860 1.6000233 6.4062476 150-159
TCTGG 6431150 1.5911354 11.846368 180-189
TGCTC 5746835 1.5881808 5.3717947 150-159
TTGGT 6065140 1.5840818 24.385496 25-29
GAGAA 3774875 1.584029 10.020191 160-169
AATAG 3189985 1.5784112 9.135733 240-247
TAGTT 4374750 1.5779824 9.666511 220-229
GTGCT 6345450 1.5699323 7.849855 220-229
ACTGA 3904435 1.5625365 8.434913 180-189
CTGGT 6297445 1.5580553 6.818113 160-169
TTGTA 4318530 1.5577037 8.657918 210-219
CAAAG 3314865 1.5537428 28.495363 70-79
GAACC 4066590 1.541644 12.195008 70-79
GGCCG 7753075 1.5410024 12.992339 120-129
ATAGA 3114065 1.5408458 9.491747 240-247
ACCAT 3424215 1.5306844 11.647188 45-49
GCTCA 4718405 1.5272413 10.956702 140-149
CCACC 4429130 1.5169293 9.588125 230-239
GGACG 6161915 1.5142744 6.983439 170-179
CCCAC 4375465 1.4985496 10.157099 240-247
CAGAC 3878960 1.4705135 16.060574 60-69
GCTCT 5296040 1.4636 9.847774 130-139
AAATA 2141090 1.4631155 5.181941 9
CCTCG 5578135 1.4602995 9.501343 130-139
AGATC 3635240 1.454806 7.1008134 130-139
CATGT 4244805 1.450402 7.8404937 70-79
GCTGA 5000385 1.4489827 21.314165 30-34
CGACT 4349740 1.4079129 8.361186 190-199
CACTG 4338380 1.4042357 7.01104 230-239
TATAC 2952155 1.393099 6.589708 110-119
TGATA 3262955 1.3784827 7.3991055 180-189
TCGAC 4257590 1.3780857 9.000608 190-199
CTCTT 4215350 1.3736486 7.8093143 200-209
CCTCA 3798445 1.3733175 7.4393067 130-139
TGGAC 4738275 1.3730302 10.054797 170-179
AATTG 3248660 1.3724434 8.963024 170-179
CTGCT 4961370 1.3711113 5.6952763 90-99
GCGTA 4698495 1.3615028 10.066147 210-219
GGGAG 6132130 1.3491106 6.361464 150-159
GAGGG 6121695 1.3468148 7.942008 200-209
CCATC 3721360 1.3454475 10.763553 240-247
GTGGA 5158680 1.338276 21.60252 25-29
ACTTC 3493695 1.3334242 6.6696887 220-229
GTAGG 5104690 1.3242699 5.194945 160-169
CCATG 4067295 1.3164917 7.9962373 70-79
CTTGA 3847250 1.3145621 8.001858 200-209
GATCT 3847250 1.314562 5.6226034 130-139
ACGGA 3868670 1.312994 9.125369 200-209
TCCAC 3623050 1.3099039 9.3918085 240-247
CTGAT 3828090 1.3080151 7.1352706 180-189
ACCGT 4037960 1.3069965 19.10039 45-49
CTCGA 3988445 1.2909697 8.032301 190-199
ATCGG 4434370 1.2849661 7.867906 190-199
TATGG 4192075 1.2823514 5.9183035 180-189
GAATT 3026365 1.2785319 9.967321 170-179
GTGTT 4881760 1.2750087 7.4137444 160-169
AGCCG 4603470 1.2636503 9.0374775 120-129
GTCCA 3894565 1.2605829 16.75625 70-79
GGAGT 4850955 1.2584453 6.876688 210-219
GGAAT 3508800 1.2571232 8.40161 170-179
GGAGG 5676055 1.248771 16.247618 30-34
GGCTC 5299980 1.2421513 8.195288 130-139
GTGGT 5603985 1.2412602 10.96212 50-59
ACGTG 4271430 1.2377503 9.005186 170-179
ACTAT 2620480 1.2365843 6.0487614 230-239
ATAAC 2222540 1.2283827 7.4372 80-89
TCACG 3789300 1.2265109 9.311739 200-209
AAGTA 2474090 1.2241849 15.458242 70-79
TTGGC 4940855 1.2224206 8.374443 60-69
AAAGT 2462905 1.2186505 20.895775 70-79
TGGTC 4902640 1.2129656 12.393987 50-59
ATAAA 1774760 1.2127836 5.453123 7
GCATA 3006730 1.2032793 7.926062 210-219
ACTCG 3711395 1.2012948 8.242471 190-199
TGCCC 4549260 1.1909504 6.9602947 60-69
TTCCA 3104075 1.1847194 7.507507 230-239
TTGAG 3842630 1.1754565 7.73734 200-209
ACTGG 4051275 1.1739551 6.9176116 160-169
ATACC 2613560 1.1683073 7.6440315 70-79
AGCTT 3357360 1.1471722 5.8938727 150-159
ACTGT 3355395 1.1465006 5.3913693 230-239
TCTGT 3916675 1.1426336 6.5786586 210-219
AGAGC 3365115 1.1420918 6.7903757 140-149
AAAAG 1958115 1.1347781 14.075978 70-79
TATCA 2402915 1.1339169 6.86269 70-79
CCATA 2509975 1.1220031 10.568195 70-79
GAAGA 2658495 1.115569 18.275991 35-39
AGTTC 3263400 1.1150671 5.3920336 160-169
CAATA 2009345 1.1105514 6.507856 100-109
AGGTT 3603395 1.1022749 23.116827 10-14
ATATC 2330800 1.0998865 7.3629227 70-79
ATCCA 2433900 1.0879961 14.886488 240-247
TCTAC 2836605 1.0826354 6.961126 180-189
CTGAA 2700775 1.0808374 14.601662 35-39
CGGCT 4611535 1.0808011 7.6869917 130-139
GTCTA 3153380 1.0774745 7.038939 180-189
CTCGG 4577205 1.0727552 8.208111 130-139
TATGT 2973900 1.0726925 7.338135 220-229
GCTTC 3849975 1.0639694 5.2919664 150-159
CTGCC 4033965 1.0560513 5.832573 200-209
ATGGG 4065705 1.054734 30.98348 15-19
AGAGA 2505015 1.0511651 13.021159 35-39
TACTG 3074035 1.0503632 6.2135515 180-189
GAGCT 3611340 1.0464733 6.086593 140-149
CTTCT 3197640 1.0420094 5.588056 210-219
CTACC 2869830 1.0375792 8.328887 230-239
AACTT 2197055 1.0367733 5.9310226 200-209
CTGCG 4419620 1.035822 6.7248974 210-219
CATGG 3522265 1.0206616 5.3279095 190-199
TAGAT 2413645 1.0196793 7.3686457 240-247
AAAAT 1489015 1.0175195 5.364825 8
GCGGT 4834080 1.0142883 5.6569705 130-139
GGGGT 5375715 1.0097902 7.7178597 10-14
TGCGT 4029460 0.99693155 8.101432 210-219
TCCCC 3355855 0.9813158 5.0643764 80-89
ATACG 2444300 0.9781973 5.129 120-129
TATAA 1662580 0.97002935 6.1619883 240-247
AAGAG 2302270 0.96608835 13.515312 35-39
ACCCA 2269795 0.96115404 12.571786 240-247
CAGCG 3490020 0.9580087 6.128751 160-169
AGCTG 3304205 0.95747346 5.0332046 90-99
CGAAC 2525380 0.9573714 12.816255 70-79
TAGGC 3297335 0.9554827 5.8509855 160-169
CGTGG 4533095 0.9511356 7.634953 50-59
CGGAG 3861270 0.9488969 6.4870696 210-219
CCATT 2476455 0.9451784 5.8577347 50-59
ACAAT 1708905 0.94450027 7.4233537 100-109
TGTAA 2219840 0.9378036 6.609762 230-239
GAGTT 3038875 0.92958874 5.161917 160-169
ACTAA 1678430 0.9276569 11.980962 240-247
TTCTG 3165690 0.9235443 5.582556 210-219
TGCAT 2687890 0.9184218 6.1114674 210-219
ACGTC 2826210 0.9147805 11.50994 70-79
CCCAA 2149005 0.91000515 9.37593 240-247
TGAAG 2487920 0.8913653 9.666153 35-39
TGAAC 2210370 0.88457966 6.3771057 190-199
GGTGG 4692210 0.88139856 21.63719 25-29
CATCA 1967525 0.8795183 5.501317 230-239
TCGAA 2193470 0.87781626 16.250244 70-79
GAGGC 3517135 0.86432666 13.702819 30-34
CCAAT 1915480 0.8562533 10.230062 240-247
ATCAA 1543295 0.8529687 12.818773 70-79
GTATG 2784260 0.8517023 5.156428 220-229
CCAGC 2762000 0.8468723 5.5983973 80-89
CTTCC 2721800 0.8401949 5.3040338 230-239
AGCGT 2871240 0.83201134 6.3256817 160-169
CGGTC 3528195 0.82689977 5.3900204 130-139
GGGCC 4115030 0.817904 16.523422 15-19
CGTCC 3070405 0.80380106 9.51584 70-79
AAAGA 1386170 0.80332124 5.9598756 8
GACCG 2921205 0.80186933 17.690233 45-49
TAACC 1772250 0.7922269 6.0224295 80-89
AACCG 2054140 0.7787244 5.5656323 190-199
TGAGA 2166565 0.77623105 5.5989337 240-247
TGGGC 3647745 0.76537114 17.886528 15-19
GGTCC 3264465 0.7650896 12.629315 50-59
AAAGG 1777280 0.74578977 8.61102 10-14
CTAAT 1561655 0.73693293 9.233329 240-247
ATGAT 1735930 0.73336875 6.76717 240-247
AATCC 1637885 0.7321634 9.142721 240-247
AGCCA 1931085 0.73207426 5.64519 80-89
CATAC 1628845 0.72812235 9.117734 70-79
GTCGA 2465530 0.7144471 11.680557 70-79
GTAGC 2458675 0.71246076 5.507039 80-89
AAGGT 1940365 0.6951887 9.694531 10-14
GGAAG 2261625 0.6871776 6.2456694 30-34
AAAGC 1453190 0.6811388 9.644608 70-79
CATTG 1982860 0.677521 5.3375516 50-59
AGGAT 1811655 0.6490748 5.7928624 10-14
TAGGT 2117980 0.6478879 5.559613 10-14
TATGA 1504755 0.6357055 5.4544387 240-247
CAATC 1417340 0.6335759 8.72729 240-247
GAGGT 2430785 0.6305995 6.8007617 10-14
ATAGG 1748060 0.62629026 5.236176 10-14
AGAAG 1486285 0.62368125 5.6484294 9
GAAAA 1073095 0.62188625 5.644917 8
CCGTA 1909935 0.6182029 5.189551 80-89
CGATG 2101785 0.6090431 13.524907 15-19
TAAAA 871340 0.59543085 6.269654 8
AATGG 1660240 0.59482634 6.631121 10-14
GGGTC 2760340 0.5791755 5.650806 70-79
CATCC 1596545 0.5772265 7.251268 240-247
ACGAT 1433750 0.57377994 6.0163403 10-14
AAGAA 988120 0.572641 5.1754913 9
AAATG 1149455 0.5687527 5.3012886 9
GAAGG 1781500 0.5412953 5.433158 10-14
AAGCA 1125380 0.5274878 6.508961 70-79
AAGGG 1689520 0.51334786 6.2509947 10-14
TGATC 1483060 0.5067449 5.4326563 240-247
GGTCG 2329075 0.4886873 5.7291613 70-79
GATCC 1501980 0.48615706 5.519558 240-247
ATAAG 959785 0.4749036 5.394787 9
CGGTT 1808120 0.44734827 8.052233 10-14
TAAGG 1225135 0.43893808 5.894979 10-14
AAGCT 1018105 0.4074408 8.136711 30-34
TAAAG 815940 0.40372878 5.4907293 9
Produced by FastQC (version 0.10.1)