FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_18_SL248347.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_18_SL248347.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5573761
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 6382115 8.436784 59.72438 110-119
ATACA 5841035 7.3158684 36.33108 110-119
CACAG 8454655 7.296757 41.28032 110-119
TCCCT 9956390 7.0544205 106.69346 30-34
CAGTA 7191340 6.550585 38.6288 110-119
AGTAA 5525765 6.1965065 46.36589 110-119
GGAGA 8778020 6.0727777 92.61091 35-39
CCCTG 9998345 6.0093813 70.062805 30-34
GGTGA 9796905 5.8107433 101.54334 45-49
GTAAT 6029985 5.797259 39.008465 110-119
AATAC 4245680 5.3176937 38.454166 110-119
GTAGT 7364855 5.149497 26.565912 80-89
AGACG 6628270 5.121682 99.93574 40-44
ACAGT 5566475 5.070497 35.004456 110-119
GTGAC 7492795 4.9637303 83.40241 45-49
GAGAC 6122580 4.730934 140.02559 40-44
ACTCC 5684615 4.697952 121.265854 30-34
CCCCA 5991070 4.691111 100.61197 60-69
ACCAG 5325655 4.596286 92.7678 45-49
CTCAG 6175775 4.5695915 34.145252 140-149
GGCCC 8667760 4.419272 59.749516 60-69
TAGTA 4533740 4.358761 33.243916 80-89
GCACA 5029735 4.3408933 33.733723 110-119
GTGTC 7596625 4.3145647 23.771149 120-129
CACTC 5188745 4.288149 97.71298 25-29
CGTGT 7537870 4.2811947 23.123552 120-129
TGACC 5745260 4.2510443 141.8142 45-49
CTCCC 6197890 4.1607065 69.67832 30-34
AGTAG 5012330 4.087787 27.57578 70-79
GACCA 4630685 3.9964948 104.76366 45-49
ATGCA 4368580 3.9793353 94.74208 25-29
CCCAG 5595450 3.9226968 86.00032 60-69
TGAGG 6333470 3.75651 68.45293 35-39
CAGGG 6558665 3.685717 36.296623 50-59
AGGAG 5301800 3.667872 92.87968 35-39
GACGG 6490205 3.6472452 107.12108 40-44
ATGGT 5213845 3.6455133 25.378529 190-199
CCACT 4410455 3.6449444 26.950768 240-247
ACGGT 5491060 3.6376467 95.310356 40-44
TACAC 3553360 3.615189 26.64891 110-119
CTGAG 5445480 3.6074514 104.9198 35-39
TGTCC 5596300 3.5500855 20.402458 120-129
TGCAC 4778210 3.535503 114.84163 25-29
AGATA 3141945 3.5233283 19.246157 150-159
GGGTT 6883215 3.5001452 43.28413 50-59
CCCTT 4934915 3.496545 33.824802 20-24
GAGGA 5005600 3.462956 121.80586 35-39
AAAAA 1918310 3.4500647 21.975864 130-139
GCCCC 6015870 3.4258149 73.1839 60-69
TGGTG 6639770 3.3763523 17.928106 190-199
CCTGA 4561855 3.3754165 83.90566 30-34
CCAGG 5308265 3.331814 41.13683 50-59
GGATG 5573585 3.3058064 69.61737 20-24
TCTTG 4894900 3.277313 17.911026 170-179
GCACT 4425170 3.2742803 88.737144 25-29
CTTCA 3742725 3.2646124 16.574871 200-209
CCGTG 6053180 3.2573447 23.282011 120-129
CTCTC 4578590 3.244077 13.581418 130-139
AGGCT 4888780 3.238656 23.743158 30-34
CTTGG 5697695 3.236052 19.352589 20-24
CAGAT 3521735 3.207945 17.927795 150-159
CCTGG 5951895 3.2028408 29.877201 50-59
TCAGG 4805330 3.1833735 20.592764 140-149
TGTCT 4650875 3.1139295 19.725897 180-189
TCGCA 4198505 3.106566 23.777855 100-109
TCTCA 3506775 3.0588038 21.485924 130-139
TTCCC 4297545 3.044948 50.447937 50-59
GGTTG 5975350 3.038492 74.87408 15-19
CGCAC 4319495 3.028187 24.295994 100-109
CGGAT 4568385 3.026405 79.022865 20-24
GGTTC 5225110 2.9676435 48.765266 50-59
ACAGA 2780880 2.954613 13.735287 140-149
TGGCC 5487410 2.9528916 44.249676 60-69
AGGGT 4952380 2.937357 50.68846 50-59
GATGC 4420780 2.9286213 96.831116 25-29
ACCAC 2987590 2.8798945 19.017866 230-239
TCTCT 3793960 2.83719 15.079709 180-189
GTTGG 5570415 2.8325806 53.26873 15-19
GCCCT 4693985 2.821262 18.388435 20-24
AGTCA 3062130 2.7892914 31.920864 70-79
TCAAA 2224060 2.7856245 57.19145 70-79
CGGTG 5770735 2.7802875 68.029686 40-44
GACTC 3736090 2.7644153 18.697077 190-199
GCCGT 5122650 2.7566066 22.03226 120-129
CCAGT 3716675 2.7500494 36.857616 60-69
TTCTT 3442965 2.7174685 20.454016 170-179
GATGG 4571365 2.7113695 18.795933 15-19
CGGCC 5314055 2.7093801 12.673792 120-129
GTTCC 4257380 2.7007244 55.187233 50-59
AGATG 3201185 2.6107147 16.945097 180-189
TCACA 2530195 2.574221 11.209873 140-149
GCGGA 4479745 2.5174444 87.07344 20-24
GGCTG 5208500 2.5094073 12.452163 30-34
TGTTC 3724745 2.493852 13.001025 140-149
TCCAT 2850765 2.4865954 16.324636 70-79
TCCTC 3493305 2.475118 16.889492 130-139
TGGAG 4152245 2.4627807 16.081293 25-29
GGCGG 5992385 2.4490654 42.66953 20-24
AGGGA 3539020 2.4483519 8.668989 150-159
ACAGC 2795210 2.4123952 10.940783 120-129
GAGAT 2929455 2.3891063 16.45825 180-189
TTGGG 4697505 2.3887022 79.93129 15-19
CTGGC 4424435 2.3808823 35.865925 60-69
ACTCT 2702690 2.3574362 13.033908 200-209
GTTCT 3516295 2.354287 17.46803 160-169
AATAA 1519870 2.34351 9.699879 110-119
TACCA 2296775 2.3367393 15.867097 230-239
CACGG 3673555 2.3057632 9.441638 120-129
CTGGA 3467780 2.2972903 14.356696 180-189
CAGCT 3097470 2.291886 11.911135 150-159
CATTT 2446735 2.2525108 17.618511 150-159
TCATT 2442040 2.2481885 16.592093 150-159
TGTAG 3188745 2.2295663 7.661738 210-219
TTCAC 2540550 2.2160087 18.238659 200-209
TTCAT 2399195 2.2087445 16.055302 140-149
TCAGC 2972405 2.199348 14.172593 140-149
ACTAC 2145580 2.1829135 12.408294 230-239
GTGAA 2665245 2.1736307 17.635864 190-199
CAGAG 2793625 2.1586416 9.042988 140-149
CTACT 2474585 2.1584704 7.3744655 230-239
TTGTC 3223620 2.1583304 13.339735 170-179
TCTGC 3390290 2.1506746 12.013658 210-219
GATAC 2346060 2.1370237 14.47743 150-159
CACTA 2092275 2.128681 14.953206 240-247
TGGGG 4915740 2.1204393 52.796257 15-19
ATTTG 2568110 2.116762 15.440028 150-159
TAGTC 2665205 2.0813866 26.538818 70-79
GCTGT 3648600 2.072252 10.13556 140-149
CTCGC 3442915 2.0693214 16.22177 100-109
CTCTG 3243995 2.0578704 14.432391 180-189
TTGGA 2942680 2.0575178 15.606787 170-179
ATCTC 2354510 2.0537343 9.005938 100-109
TCTCG 3237080 2.0534837 16.650305 100-109
ACACA 1724075 2.0459547 13.384003 110-119
GTCTC 3216055 2.0401466 11.861949 180-189
GAATC 2234885 2.0357547 14.080692 190-199
GTCCT 3204485 2.032807 14.421466 130-139
ATATA 1534575 2.0286186 10.525692 110-119
TACAG 2226800 2.02839 13.580505 160-169
GTTCA 2586840 2.0201876 15.086971 140-149
GAACT 2204515 2.0080907 10.294412 160-169
GTCAA 2202885 2.0066059 40.56319 70-79
CAGGC 3184790 1.9989824 15.387443 140-149
ATGTA 2068125 1.9883058 7.1259604 70-79
AACCA 1670870 1.9828165 15.300231 70-79
AGCTC 2679215 1.9824102 12.048532 140-149
TGAAT 2058905 1.979442 13.513995 190-199
TTGCA 2525840 1.9725498 14.185458 150-159
GATAT 2048485 1.9694239 7.8415847 180-189
AATAT 1485830 1.9641806 13.235353 110-119
AATCG 2150560 1.9589429 12.980551 190-199
ACACG 2258575 1.9492544 13.090313 120-129
CCTTC 2744625 1.9446543 13.793698 200-209
TTTGC 2893070 1.9370153 12.2287855 150-159
ACGGC 3066560 1.9247736 14.227063 120-129
GAGTC 2899920 1.9211016 12.112227 210-219
TGGAA 2330210 1.9003942 9.098617 170-179
TCGGC 3519490 1.8939121 7.895513 130-139
GGGGC 4631705 1.8929607 48.190193 15-19
TACTA 1760250 1.8901767 9.782535 230-239
ATATG 1949010 1.8737881 8.043775 180-189
CAGCC 2658120 1.863478 9.972237 120-129
CATAG 2039290 1.8575872 8.229747 210-219
GGGCG 4507080 1.8420268 60.21003 20-24
CTGTT 2701620 1.8088325 12.737509 140-149
AGTAT 1867210 1.7951452 11.618107 220-229
GTTGT 2952280 1.7697425 6.753023 210-219
CGTAG 2649130 1.7549617 9.555289 210-219
GACGT 2625350 1.739208 9.514907 170-179
CTCCA 2100580 1.735988 7.7912445 150-159
CCAGA 2005705 1.7310159 25.383757 60-69
GCAGA 2238745 1.7298843 14.369103 150-159
TGCAG 2606360 1.726628 13.516575 150-159
GTCAC 2332880 1.7261493 8.298963 140-149
CACCA 1789320 1.7248193 13.169108 230-239
ATTGT 2090530 1.7231171 7.5414343 170-179
AGTCT 2181695 1.7037905 13.004403 210-219
AGAAC 1601435 1.7014831 10.884129 160-169
CAAAA 1149315 1.6790483 20.663652 70-79
GTCTG 2928750 1.6634074 9.024507 210-219
ATAGT 1728145 1.6614474 7.3282037 220-229
GGTCA 2495885 1.6534419 7.270318 130-139
CCTTG 2602765 1.6510978 21.999594 20-24
TTCAG 2106055 1.6447196 9.203839 150-159
TGGTT 2737655 1.6410856 18.087309 10-14
TCAGA 1798490 1.6382428 9.984949 130-139
TGACT 2078405 1.6231265 11.230172 190-199
TCCCA 1955470 1.6160644 10.447188 240-247
TCTGG 2836590 1.6110642 11.903068 180-189
CTGTG 2824595 1.6042516 5.279702 210-219
TGTGC 2814775 1.5986743 8.149614 220-229
AACTG 1740385 1.585315 10.160884 160-169
GCTCC 2632605 1.5822948 6.42253 150-159
GAACC 1828685 1.5782396 12.855659 70-79
TGCTC 2485990 1.5770204 5.2570925 150-159
CAAAG 1482940 1.5755851 29.055548 70-79
GAGAA 1650585 1.5701264 9.968336 160-169
GTGCT 2750950 1.5624243 7.9023776 220-229
CTGGT 2749620 1.561669 6.8514004 160-169
AATAG 1392215 1.5612081 9.283424 240-247
GTCCC 2590635 1.5570692 19.435497 50-59
TTGTA 1886860 1.5552423 8.554334 210-219
TTGGT 2590270 1.5527358 23.442678 25-29
TAGTT 1883125 1.5521638 9.422185 220-229
GGCCG 3386740 1.545981 13.122619 120-129
ACTGA 1694645 1.5436505 8.37949 180-189
ATAGA 1359765 1.5248191 9.57846 240-247
GGACG 2702845 1.5188948 7.107003 170-179
ACCAT 1482200 1.5079905 11.206091 45-49
CCACC 1924140 1.5066347 9.622746 230-239
GCTCA 2028075 1.5006174 10.834221 140-149
AAATA 966230 1.4898443 5.3567476 9
CCCAC 1901160 1.488641 10.249933 240-247
GCTCT 2334275 1.480778 10.023635 130-139
CCTCG 2443710 1.4687617 9.63244 130-139
GCTGA 2134525 1.4140527 20.268232 30-34
CACTG 1905135 1.4096512 7.0605803 230-239
CAGAC 1629995 1.4067609 14.567505 60-69
CGACT 1899515 1.4054928 8.322492 190-199
TATAC 1301950 1.3980488 6.7235727 110-119
CATGT 1789000 1.3971162 7.1524744 70-79
AATTG 1451240 1.3952295 9.140409 170-179
CTGCT 2195055 1.3924618 5.7393928 90-99
CTCTT 1848875 1.3826212 7.8713074 200-209
TGGAC 2076305 1.3754838 10.138871 170-179
GCGTA 2073145 1.3733906 10.116989 210-219
TCGAC 1855490 1.3729178 8.944022 190-199
TGATA 1421520 1.3666568 7.3241796 180-189
CCTCA 1633565 1.3500316 7.294358 130-139
CTTGA 1726490 1.348299 8.078937 200-209
GAGGG 2679590 1.3481969 7.862639 200-209
GGGAG 2666675 1.3416989 6.2855363 150-159
AGATC 1467430 1.3366807 6.8932743 130-139
ACGGA 1725000 1.3329121 9.266955 200-209
GTAGG 2232165 1.3239425 5.091929 160-169
CCATC 1596345 1.3192718 10.591506 240-247
ACTTC 1505300 1.3130063 6.5595894 220-229
GAATT 1348780 1.2967241 9.987525 170-179
CTGAT 1658400 1.2951242 7.1208844 180-189
ATCGG 1947905 1.2904232 7.740984 190-199
GTGTT 2147680 1.2874255 7.6240096 160-169
CTCGA 1737470 1.2855922 8.020133 190-199
GTGGA 2164000 1.2835124 20.460629 25-29
GGAAT 1573600 1.2833438 8.453543 170-179
TATGG 1830825 1.2801104 5.8789873 180-189
CCATG 1717270 1.2706457 7.2768226 70-79
GGAGT 2135170 1.2664126 6.9972634 210-219
TCACG 1711390 1.2662951 9.526781 200-209
TCCAC 1531750 1.2658883 9.178437 240-247
AGCCG 2006150 1.2591909 8.8768 120-129
ATAAA 811050 1.2505699 6.0299926 7
ACGTG 1882330 1.2469819 9.113303 170-179
GGCTC 2315175 1.2458447 8.282411 130-139
AAGTA 1104960 1.239085 15.539144 70-79
ATAAC 984700 1.233332 7.6810155 80-89
AAAGT 1099100 1.2325137 21.221228 70-79
ACCGT 1663790 1.2310747 17.46302 45-49
ACTAT 1142165 1.2264698 6.0202966 230-239
GGAGG 2431565 1.2234067 15.69792 30-34
GATCT 1545520 1.2069709 5.4429884 130-139
GTGGT 2355865 1.1979678 10.052301 50-59
GTCCA 1617855 1.1970866 15.437516 70-79
ACTCG 1617165 1.1965759 8.206172 190-199
TTGGC 2092670 1.1885489 7.9532905 60-69
GCATA 1303905 1.1877258 7.984298 210-219
TTGAG 1685160 1.1782616 7.7234197 200-209
TGGTC 2071045 1.1762668 11.42385 50-59
TTCCA 1343175 1.1715919 7.351307 230-239
ACTGG 1759925 1.1658926 6.8982406 160-169
AAAAG 882955 1.1548914 13.719569 70-79
TATCA 1072100 1.1512332 6.8453465 70-79
AGAGC 1485480 1.1478344 6.6534405 140-149
ACTGT 1468955 1.1471776 5.520238 230-239
TGCCC 1908560 1.1471164 6.3925276 60-69
AGCTT 1460400 1.1404966 5.907385 150-159
TCTGT 1687610 1.1299161 6.5309644 210-219
ATACC 1108150 1.127432 6.8582616 70-79
ATATC 1043030 1.1200174 7.2715473 70-79
CAATA 888360 1.1126666 6.4357724 100-109
GAAGA 1169000 1.1120166 18.227375 35-39
AGTTC 1419330 1.108423 5.269452 160-169
AGGTT 1568055 1.0963819 22.879416 10-14
CGGCT 2026290 1.0903896 7.79663 130-139
CCATA 1069035 1.0876364 9.573121 70-79
CTGAA 1192485 1.0862334 14.39263 35-39
TCTAC 1243515 1.0846628 6.934776 180-189
CTCGG 2014015 1.0837841 8.416449 130-139
GTCTA 1376675 1.0751117 7.0345707 180-189
TATGT 1299030 1.0707241 7.425313 220-229
AAAAT 689940 1.0638288 5.1058593 8
GCTTC 1669800 1.0592595 5.3262677 150-159
CTGCG 1955875 1.0524977 6.7818937 210-219
CTTCT 1405865 1.0513306 5.3908734 210-219
TACTG 1344735 1.0501683 6.164194 180-189
CTGCC 1740455 1.0460789 5.859852 200-209
AGGGG 2077370 1.0451987 5.1426783 60-69
AGAAA 797345 1.0429149 5.0036774 6
AGAGA 1094490 1.0411385 12.510734 35-39
GAGCT 1568410 1.0390201 6.0100574 140-149
GGGGT 2405335 1.0375583 7.874606 10-14
ATCCA 1019005 1.0367359 14.427118 240-247
CTACC 1250770 1.0336772 8.450277 230-239
CATGG 1536095 1.0176126 5.330056 190-199
GCGGT 2107215 1.0152369 5.6846743 130-139
ATGGG 1711415 1.015075 29.392925 15-19
AACTT 937890 1.0071169 5.865472 200-209
ATACG 1094955 0.9973934 5.141056 120-129
TAGAT 1036555 0.99654925 7.4494276 240-247
TGCGT 1754125 0.9962696 8.118606 210-219
ACGTC 1333110 0.98639745 11.052069 70-79
CAGCG 1570070 0.9854786 6.3826065 160-169
CGAAC 1137165 0.9814259 13.435559 70-79
AAGAG 1030740 0.9804961 13.510182 35-39
TATAA 741230 0.9798628 6.170846 240-247
TCCCC 1432430 0.96160483 5.0349054 90-99
CGGAG 1701620 0.95624495 6.628076 210-219
ACAAT 754530 0.94504523 7.4272475 100-109
TTCTG 1402435 0.9389812 5.4256725 210-219
TGTAA 976325 0.93864393 6.512066 230-239
TAGGC 1414010 0.9367351 5.760164 160-169
ACTAA 741495 0.9287189 12.201365 240-247
ACCCA 962780 0.92807406 12.387437 240-247
CCATT 1057155 0.9221093 5.728228 50-59
GAGTT 1312570 0.91774714 5.0735984 160-169
TGAAC 1001530 0.9122927 6.523797 190-199
TGCAT 1168085 0.9122138 6.1126795 210-219
TCGAA 1000300 0.9111723 16.928852 70-79
CGTGG 1889760 0.9104691 7.000891 50-59
CCCAA 932220 0.8986158 9.249835 240-247
CATCA 870260 0.8854027 5.618843 230-239
GTATG 1255615 0.8779244 5.183296 220-229
AGCGT 1309365 0.86741126 6.4966283 160-169
TGAAG 1061990 0.8661021 9.544763 35-39
GGTGG 1988050 0.85755944 20.57767 25-29
CCAGC 1208115 0.84695035 5.6003003 80-89
CGTCC 1405275 0.8446232 9.2773695 70-79
CCAAT 828400 0.8428143 10.081279 240-247
GAGGC 1495590 0.84046394 13.254246 30-34
ATCAA 670780 0.84014875 12.483576 70-79
AAAGA 635295 0.830956 5.567106 8
CGGTC 1538325 0.82780534 5.3520384 130-139
CTTCC 1161215 0.82275784 5.2419767 230-239
TAACC 785585 0.7992543 6.2040987 80-89
GGGCC 1748335 0.798081 15.722802 15-19
AACCG 917775 0.79208213 5.693583 190-199
TGAGA 956395 0.7799845 5.4069433 240-247
GACCG 1233670 0.7743319 16.298748 45-49
AAAGG 790810 0.7522616 8.5828085 10-14
TGGGC 1545540 0.744627 17.042591 15-19
GTCGA 1117990 0.7406317 12.257809 70-79
CTAAT 683800 0.7342722 9.387979 240-247
GGTCC 1364200 0.73410493 11.735984 50-59
AGCCA 848365 0.7321781 5.8090744 80-89
ATGAT 756950 0.7277356 6.725623 240-247
AATCC 689265 0.7012583 8.810437 240-247
GTAGC 1057610 0.7006319 5.3243084 80-89
GGAAG 1001740 0.6930201 6.0960283 30-34
CATAC 680350 0.6921882 8.172471 70-79
AAAGC 638790 0.6786978 9.438557 70-79
AAGGT 831770 0.67834705 9.671162 10-14
GAAAA 513370 0.67148 5.927368 8
CATTG 849275 0.66323966 5.202569 50-59
TAGGT 929465 0.64988065 5.369851 10-14
AGGAT 794395 0.64786595 5.744725 10-14
TATGA 662395 0.6368299 5.3829627 240-247
GAGGT 1067105 0.6329216 6.650004 10-14
AGAAG 658215 0.62613 5.0928035 9
CAATC 610205 0.62082267 8.578104 240-247
TAAAA 399105 0.6153859 6.3666115 8
ATAGG 753555 0.614559 5.2363205 10-14
GGGTC 1265750 0.60982674 5.994146 70-79
CGATG 894605 0.5926464 13.006179 15-19
AAGAA 452805 0.5922619 5.059358 9
AATGG 717775 0.58537877 6.380633 10-14
AAATG 511230 0.57328534 5.12006 9
CATCC 679990 0.561966 7.023489 240-247
AAGCA 503015 0.5344404 6.323686 70-79
GAAGG 764000 0.52854776 5.202433 10-14
AAGGG 752090 0.5203082 6.2635264 10-14
GGTCG 1057490 0.50948894 6.08482 70-79
TGATC 644100 0.50300866 5.420047 240-247
GATCC 642290 0.47524452 5.4663944 240-247
ATAAG 418480 0.4692769 5.3070245 9
ACGAT 498655 0.45422438 5.6177177 10-14
CGGTT 793530 0.45069182 8.010957 10-14
TAAGG 536330 0.43740195 5.6669736 10-14
AAGCT 434980 0.39622286 7.5898232 30-34
TGGGT 775930 0.39456382 5.0608997 10-14
Produced by FastQC (version 0.10.1)