FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_14_SL248343.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_14_SL248343.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11853962
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 51704 0.4361748417955111 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 43360 0.36578487428928824 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 40832 0.34445867128644414 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 39034 0.3292907468405922 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 37404 0.3155400700626508 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 37061 0.3126465227406668 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 34059 0.28732165667478937 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33873 0.2857525610424599 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33854 0.2855922770800176 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 33587 0.2833398656078027 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 32530 0.27442301569719896 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 31718 0.26757298530229806 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 30986 0.2613978347492594 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 30654 0.25859708340553145 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29984 0.2529449647299359 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29915 0.2523628808663298 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29816 0.2515277170620253 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29362 0.2476977739594576 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 28054 0.23666348854501137 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 28013 0.236317612626057 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27532 0.23225989757686083 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27359 0.2308004699188339 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27179 0.22928199027464402 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26947 0.22732483873324377 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26774 0.22586541107521688 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25675 0.21659424924763548 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25532 0.21538790153030693 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24508 0.20674943955447134 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24197 0.20412584416923218 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23822 0.20096234491050335 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 23794 0.2007261369658516 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23194 0.19566453815188542 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22966 0.19374113060257828 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22799 0.19233231893269104 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22700 0.1914971551283866 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22626 0.1908728912746641 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22392 0.1888988677372173 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 21838 0.18422532483232187 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21609 0.18229348128499145 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 20693 0.17456610709566978 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20608 0.17384904726369124 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20434 0.17238118360764107 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19942 0.16823067258018878 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 19074 0.16090822629598442 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18897 0.1594150546458644 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18803 0.15862207083167637 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18317 0.15452217579236377 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 18211 0.1536279600018964 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17973 0.1516201924723565 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17828 0.15039697275898134 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17633 0.14875195314444234 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17513 0.1477396333816491 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 17468 0.14736001347060163 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17011 0.14350476237396406 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 16876 0.1423659026408217 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 15656 0.13207398505242382 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15527 0.13098574130742108 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15393 0.12985531757230198 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15187 0.12811750197950694 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 15122 0.12756916210799393 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14803 0.12487807873856858 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14196 0.11975742793843948 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14008 0.11817146031006343 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13763 0.11610464079436057 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13157 0.11099242599225474 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 13157 0.11099242599225474 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12290 0.10367841570607364 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 24161465 7.684658 67.867424 35-39
TATTA 11927510 7.3396716 128.25531 240-247
TGGAT 18311210 7.206867 80.52874 35-39
TCACC 18669240 6.4706664 75.42465 160-169
TCTCC 16729645 6.188551 88.44448 20-24
CTGTG 17519440 5.947047 88.66459 25-29
TCCCT 15676015 5.798797 72.74608 15-19
CTGAG 17848295 5.676727 51.465332 15-19
AGTGG 18579045 5.6606226 40.1193 100-109
TGTAT 10908575 5.54608 60.066807 240-247
TTACT 9949105 5.280349 111.45149 240-247
ATTAC 10021985 4.9837065 105.66621 240-247
CCTGA 14931970 4.957685 53.245907 15-19
TGGGT 15198435 4.9421906 45.90835 100-109
TTCAC 11144915 4.779993 75.88286 40-44
CTCTG 13479295 4.7764897 74.49667 35-39
CTCCA 13516080 4.6846066 42.29225 80-89
GACAC 14924350 4.6427727 47.731575 230-239
ACACG 14889265 4.6318583 50.129337 230-239
TGAGA 12311725 4.540132 96.76483 15-19
ATTCA 9029145 4.4899898 114.59586 40-44
TCTGG 12784725 4.3398285 136.5247 35-39
AAGGG 15161215 4.328077 39.75835 90-99
TACTG 10403120 4.274187 78.54665 240-247
AGCCT 12752770 4.2341514 52.47401 30-34
CAGCC 15651225 4.1993504 56.893353 30-34
GAAGG 14632510 4.1771474 31.618177 150-159
CCCTG 14578505 4.1747127 85.82732 10-14
CCAGG 16227940 4.170965 53.73097 80-89
ACCAT 10274615 4.1289177 67.65119 160-169
GAGAC 13804235 4.113711 85.76746 8
TGGAG 13494345 4.1114273 38.678326 90-99
TCCAG 12263560 4.0717244 41.70708 80-89
TCAGT 9710930 3.9897969 95.48475 7
GGGTC 15166110 3.9853523 128.18796 8
CACCA 12190005 3.9586437 57.843533 160-169
GTATT 7762030 3.94633 69.042694 240-247
ACTAC 9770935 3.9265106 34.595528 130-139
TACTA 7828100 3.8927376 29.049767 130-139
CTGGG 14695220 3.8616118 135.63618 3
GACTC 11611580 3.8552554 53.572845 20-24
GATTC 9352690 3.842612 60.387894 40-44
AGACT 9876905 3.8021655 71.14848 15-19
CACCT 10910545 3.781541 44.0084 40-44
ACTGT 9142425 3.756223 67.45256 240-247
TGAAG 10163890 3.748086 40.42491 150-159
GGACA 12344375 3.678668 28.54436 220-229
CAAGA 10073785 3.6334817 44.524876 180-189
ACTGG 11223485 3.569678 44.52417 60-69
ACTCT 8292220 3.556488 70.58806 20-24
GCTGG 13527970 3.5548813 33.523106 90-99
CCTGT 9937855 3.521554 63.06264 25-29
TCCTG 9608655 3.4048994 64.863144 20-24
TATCT 6349145 3.3697197 49.859673 200-209
GAGTG 11030770 3.3608305 38.869576 100-109
CTCCT 8990320 3.3256564 95.47203 20-24
GGATT 8372760 3.2953234 57.74731 40-44
AGACA 9135185 3.2949412 37.571404 170-179
GGTCC 11956605 3.2799022 144.20738 9
CTGCA 9793820 3.2517257 45.328865 25-29
CATCT 7523230 3.2266724 33.863853 170-179
AGCTG 9947030 3.163696 35.80007 60-69
GTCTC 8853045 3.1371434 33.433662 30-34
CCATC 9012385 3.1236482 34.838924 45-49
GTCCC 10782165 3.0875893 76.8648 10-14
GGAGT 9861860 3.0046897 36.984528 90-99
CCTCA 8632535 2.9919941 81.088715 5
GGCTG 11343585 2.9808688 34.79241 90-99
AAGAA 7046620 2.9468591 37.411846 180-189
AGTAG 7816145 2.8823202 28.934784 50-59
AGGGA 10004535 2.8559978 40.501274 80-89
TGTGC 8305295 2.8192673 58.442165 25-29
GTGAA 7644495 2.8190217 39.4954 150-159
ATCTG 6812195 2.7988331 37.279476 200-209
ATCTC 6507655 2.7910976 49.198544 170-179
GACAA 7737515 2.79082 25.44932 180-189
GAGCT 8717600 2.7726705 29.041616 60-69
GAACA 7644335 2.757211 35.839424 210-219
CAGGG 11150875 2.745501 36.451164 80-89
GGAAG 9591010 2.7379487 30.325409 80-89
ATGAA 6093145 2.7195678 28.448814 210-219
ACCTT 6288700 2.6971889 71.37332 40-44
GTGTA 6794400 2.6741178 35.461605 230-239
CTATG 6423275 2.6390433 26.945492 50-59
CGCCA 9746825 2.615152 40.590225 70-79
CACGG 10089750 2.593305 44.882755 230-239
GGGAA 9083620 2.5931036 34.01407 80-89
TGCAA 6711140 2.5834877 36.280945 200-209
GAGTC 8034545 2.5554216 27.05418 150-159
AGAAC 7073090 2.5511706 30.799295 190-199
AGGGC 10270505 2.5287416 33.186234 150-159
AGGCT 7873090 2.5040703 20.522217 80-89
CCAAG 7994615 2.4870217 38.950993 180-189
CAGTG 7817755 2.486471 73.84782 8
CCCTC 8271090 2.4725034 26.719595 20-24
ACGGC 9608680 2.4696589 49.25794 230-239
CACTG 7372555 2.447822 19.753939 60-69
CTACA 6083470 2.44468 19.60721 190-199
TATAT 3946510 2.4285107 24.294209 240-247
ATCAG 6299770 2.4251287 26.715128 45-49
TCTGT 5526920 2.4235556 23.924976 210-219
TGAAC 6241255 2.4026031 38.60975 210-219
CTCTC 6484100 2.3985674 100.293724 20-24
GTGGA 7843920 2.3898685 26.153849 100-109
CCGCC 10270910 2.3768096 34.768383 70-79
GCAGC 9234725 2.3735433 35.154118 30-34
ACAGC 7615675 2.3691382 27.46592 210-219
CAAAT 5058060 2.3566926 36.223507 200-209
GGGCT 8921610 2.3444216 42.873596 90-99
CTACT 5465155 2.343975 22.523535 50-59
CTTCA 5457445 2.3406682 50.859116 45-49
CTGTC 6562390 2.3254325 47.632057 30-34
CAGGC 8908135 2.289602 33.77233 70-79
GTATC 5568005 2.28765 23.35509 200-209
GGCCG 10750935 2.283025 24.810158 230-239
TATGC 5544070 2.2778158 20.61601 50-59
AAATG 5086110 2.2700956 34.319168 200-209
GCCTC 7923000 2.2688365 70.97358 4
TCCAA 5638945 2.2660449 42.190754 180-189
ACAAC 6012035 2.2636657 21.158535 180-189
AATGA 5071125 2.2634072 26.80397 210-219
GCCTG 8107765 2.2240994 31.153461 210-219
TGGTG 6824430 2.2191517 33.495083 35-39
GCTCC 7741375 2.2168262 34.69206 80-89
GGGGG 11279920 2.1981096 96.89636 5
CAGTA 5636105 2.1696475 21.414875 45-49
TACAT 4359645 2.167953 18.405619 190-199
GTGGT 6612960 2.1503866 30.431787 120-129
CCTTC 5757810 2.1299016 55.51895 45-49
TGCAG 6673800 2.1226308 44.48881 30-34
GTAGT 5381275 2.117945 25.118982 120-129
GTGGG 8408105 2.1165578 30.514982 100-109
CCTCT 5715640 2.1143024 50.00086 35-39
GCAGA 7008460 2.088546 24.613623 140-149
AACAG 5783280 2.0859528 34.34295 210-219
TATCA 4173615 2.0754445 16.714125 170-179
TCTAT 3891420 2.065317 19.506824 110-119
GCTGT 6079695 2.0637774 19.570099 230-239
GCTAT 5002240 2.0552018 32.78175 50-59
CAGAG 6859750 2.04423 33.294064 170-179
TGCAC 6092585 2.0228488 26.447472 25-29
CTCAC 5794890 2.0084803 53.95982 20-24
ATGGA 5405370 1.9933106 12.274588 100-109
TGTGA 5057820 1.9906403 18.172903 210-219
GGCTC 7243845 1.9871112 19.62763 40-44
TCCGC 6914550 1.9800562 42.858036 70-79
AACTA 4243525 1.9771775 35.485283 130-139
TTCAG 4765610 1.9579809 47.816795 45-49
TGAGC 6132055 1.9503267 17.28868 200-209
CTGTA 4746640 1.950187 24.485521 190-199
TCTCT 4246995 1.9440709 32.84851 35-39
AGAGT 5270345 1.9435182 29.89886 150-159
AGCAC 6229675 1.9379716 20.203453 130-139
GGTCT 5694260 1.9329402 35.462215 15-19
CATAT 3885935 1.9323878 13.659943 160-169
CCGTG 7020965 1.9259714 19.603746 230-239
ATATC 3868515 1.9237251 13.435609 170-179
CCAGA 6171115 1.9197543 35.017265 170-179
CAGAC 6169680 1.919308 26.923843 140-149
GCCAG 7456165 1.9164113 35.911865 70-79
TGGGG 7592050 1.9111336 125.9522 4
AGAGA 5491560 1.8974285 28.917912 170-179
TGGTA 4811490 1.8936905 30.27402 120-129
ACTAT 3807845 1.8935554 17.041525 130-139
AGGAC 6347950 1.8917117 21.182608 220-229
GATGG 6123295 1.8656322 20.921785 100-109
GCACA 5986345 1.8622748 18.217342 130-139
TCCAT 4339930 1.8613724 26.639614 45-49
CTATT 3497825 1.856422 18.6587 110-119
GGATC 5821415 1.8515261 18.128061 70-79
ACCCT 5322360 1.8447038 42.574448 10-14
GTCAC 5555725 1.8446016 37.605705 160-169
TGGGA 6044190 1.8415306 16.63117 100-109
ACCAG 5904430 1.836792 13.8070965 190-199
CCTGC 6397140 1.8318902 49.20346 20-24
GCCGA 7121385 1.8303648 19.579594 220-229
CAGTT 4447130 1.8271314 24.056175 190-199
TAGTA 3833450 1.8261145 17.697073 120-129
GAGGA 6390590 1.8243237 27.546595 220-229
GGTGG 7238275 1.8220785 17.711418 40-44
TTCCA 4242455 1.8195655 22.718374 180-189
CACAG 5833075 1.8145944 15.919092 140-149
TGTCT 4093130 1.7948382 48.972145 30-34
ATACT 3598440 1.7894231 26.897758 130-139
GCTGA 5613475 1.7853897 26.202185 200-209
TCAGC 5358415 1.7790911 11.942079 45-49
CAAGG 5944725 1.7715493 17.25115 80-89
GCAAA 4907885 1.7702092 32.82965 200-209
TTCTG 3995130 1.7518653 42.16139 30-34
ACCTG 5255865 1.7450427 30.187206 25-29
AGTCA 4519020 1.73962 28.65375 160-169
CATGA 4502705 1.7333394 23.51082 60-69
TAGTG 4400335 1.7318695 25.742538 120-129
CCTGG 6291455 1.7258544 34.93621 2
AGTTC 4188540 1.7208883 21.191704 190-199
TGACC 5182870 1.720807 14.06898 210-219
TACAA 3681820 1.7154633 17.091652 130-139
CTGAA 4455330 1.7151021 22.745337 200-209
GTGCA 5390825 1.714575 51.42453 25-29
CTCAG 5095090 1.6916624 77.37814 6
ATGCA 4386895 1.6887577 16.777685 60-69
AACAC 4479305 1.6865585 22.850063 190-199
GGATG 5531755 1.6854029 22.4896 100-109
AGGGG 6982530 1.6468914 33.499863 90-99
ATTGG 4154785 1.6352265 19.740204 100-109
CGATT 3974975 1.6331434 36.003944 160-169
TCTGC 4607290 1.632628 33.883698 200-209
AGCTA 4239760 1.6321174 25.27565 50-59
GGGGC 7986115 1.6245737 29.082336 90-99
GGAGC 6574565 1.6187497 12.930807 200-209
TCTCA 3742965 1.6053371 26.517744 100-109
GGGCC 7550710 1.6034379 27.918407 2
TCTGA 3865250 1.5880625 21.351408 220-229
GCCGT 5779820 1.5855042 28.876627 230-239
GCACT 4654450 1.5453618 25.388348 25-29
ACAAT 3315340 1.5447102 23.019062 180-189
AGTGA 4167570 1.5368537 85.39756 9
CAGCT 4622515 1.5347589 12.408003 50-59
GTCCG 5589405 1.5332699 35.23884 70-79
CTCCC 5103330 1.5255548 13.538483 190-199
CCATG 4584100 1.5220045 15.743845 160-169
AGTTA 3190160 1.5196749 16.020956 50-59
CGTCC 5300610 1.5178868 18.584743 180-189
GTTCT 3448455 1.5121481 22.249432 190-199
GGCTT 4444890 1.5088364 21.230145 25-29
CGGCC 6769565 1.5006717 31.326857 230-239
TTCTC 3254165 1.4896008 20.984365 190-199
TTAGT 2918970 1.4840472 25.380173 110-119
GAGCA 4959410 1.477922 7.699428 200-209
ACTCC 4235625 1.4680468 17.306559 140-149
CATCA 3652335 1.4677134 31.211515 45-49
TACGC 4353265 1.445363 25.107588 140-149
CTTTA 2715480 1.4412032 41.54181 45-49
GGGGT 5663600 1.4256881 120.379295 7
CTGGT 4193945 1.423652 31.183008 35-39
GTTAC 3455240 1.4196073 15.043963 50-59
GTGAC 4442730 1.4130296 14.521339 210-219
CTCAA 3484080 1.400099 18.522541 150-159
GGCAG 5648670 1.3907814 13.611393 150-159
GAACT 3605060 1.3877864 15.1047325 190-199
GTCCA 4136500 1.3733929 28.289127 180-189
CAACT 3408840 1.3698634 16.814959 130-139
CTACG 4117540 1.3670981 16.36254 130-139
GAGAG 4776835 1.3636446 26.115227 220-229
TTATT 2072245 1.3609678 21.942474 240-247
GGCCT 4958700 1.3602568 67.84298 3
TAGCA 3527225 1.3578233 11.365238 130-139
CAACC 4180095 1.3574651 15.311336 140-149
CGACA 4360825 1.3565965 16.441853 70-79
ATACA 2904215 1.3531554 22.547182 35-39
AGCAG 4519815 1.3469211 12.579699 50-59
TGGAA 3648595 1.3454739 19.284842 120-129
AACCC 4134810 1.342759 19.880703 140-149
ATCTA 2694195 1.3397624 20.24136 110-119
AAGCT 3464265 1.3335866 15.973943 200-209
TCACT 3082030 1.3218658 13.933015 190-199
ACAGT 3431480 1.3209659 12.064964 120-129
CCATA 3278480 1.3174773 14.055938 160-169
CGTGT 3876755 1.3159804 23.21012 230-239
CAATT 2643110 1.3143591 23.850597 180-189
ATTAT 2133175 1.3126631 9.911812 50-59
CACGT 3941050 1.3085 16.535995 180-189
ACATG 3382875 1.3022552 14.503786 190-199
TCCGT 3672725 1.3014579 13.878776 150-159
ACAGA 3604585 1.3001264 20.434164 140-149
TCAAG 3373805 1.2987635 22.631546 150-159
GTGGC 4926495 1.2945851 14.022696 40-44
GTGCG 4911495 1.2906433 16.776117 240-247
TGTGT 3068090 1.2887758 15.303808 240-247
ATATT 2083190 1.2819047 25.914478 240-247
CCTTT 2792185 1.2781286 47.62068 45-49
AATTC 2567405 1.2767125 23.816696 180-189
AAGAG 3680585 1.2717054 18.251446 150-159
TGTCC 3587635 1.2713056 52.920273 15-19
AGAGC 4241975 1.2641237 24.841127 220-229
ACATA 2711565 1.2633944 20.92076 130-139
TTACA 2536840 1.2615134 9.206839 120-129
TGCGA 3962700 1.2603538 13.936034 240-247
CCTAC 3631410 1.2586288 17.229422 190-199
CCAGT 3781925 1.2556677 18.341772 190-199
AACCA 3332890 1.2549076 17.401321 190-199
CGGCT 4515285 1.2386204 21.743912 230-239
AGACC 3958870 1.2315534 97.40461 9
ACACA 3257775 1.2266252 7.8356996 130-139
CTTCT 2671425 1.2228504 23.07231 30-34
TGGAC 3841045 1.221661 15.8126955 80-89
AGTCG 3809325 1.2115723 15.41474 150-159
ACGTC 3643405 1.2096766 18.349304 180-189
GATTG 3071230 1.2087648 23.051575 100-109
ATCCG 3630820 1.2054982 23.447477 70-79
TGAGT 3030100 1.192577 20.898273 90-99
TACCA 2952100 1.1863196 10.556205 45-49
AAGGC 3935310 1.1727364 28.791552 25-29
GTCGA 3686280 1.1724372 12.005466 150-159
ATTAG 2459475 1.1716033 25.405869 110-119
ACGCA 3744400 1.1648345 21.288063 140-149
GAAGT 3152515 1.1625369 13.610082 140-149
GATCC 3484675 1.1569754 23.301422 70-79
GGGTG 4585735 1.1543591 14.49586 60-69
GGACT 3622295 1.1520866 12.729365 90-99
ATCAT 2311460 1.1494368 15.396234 110-119
AGGCC 4441575 1.1415902 13.835518 2
ATTCC 2658065 1.1400294 22.125004 180-189
CTTCG 3213780 1.1388272 102.95069 3
TCGGA 3574075 1.13675 91.720764 5
CCGAT 3397430 1.1280084 24.412077 160-169
GATCT 2739665 1.1256087 9.324789 220-229
CGCTG 4085785 1.1208011 12.071055 190-199
GCAAG 3758540 1.1200583 17.079462 25-29
TCGAG 3514965 1.1179497 11.636496 150-159
AGCTC 3336995 1.1079429 12.12481 210-219
ACAAG 3071290 1.1077739 17.507704 80-89
AGGTC 3479155 1.1065602 17.972414 10-14
TTGGG 3396800 1.1045634 14.537452 100-109
GAACC 3534585 1.0995638 10.144854 190-199
GACCC 4065295 1.0907515 41.805355 10-14
CTTGA 2652630 1.0898498 22.174261 90-99
CGCAG 4237575 1.0891572 16.64212 140-149
TTGAG 2755965 1.0846837 20.623798 90-99
GCTTC 3060230 1.0844154 26.802471 2
TTCGG 3179765 1.0793846 98.70387 4
CACAT 2681515 1.0775833 13.793705 130-139
ATGGG 3535715 1.0772538 19.975779 100-109
GCCCC 4654755 1.0771652 12.352741 80-89
GTATA 2255035 1.0742158 10.078316 240-247
ATCAA 2278555 1.0616428 12.228197 110-119
ACACC 3264480 1.0601237 13.241267 40-44
CTCCG 3691075 1.0569793 10.036201 140-149
GCACC 3935940 1.0560445 10.935219 130-139
TAGAC 2731055 1.0513335 16.595747 170-179
CAGCA 3378235 1.0509254 12.530951 45-49
ATGAG 2848210 1.0503198 20.2146 60-69
CTATA 2111105 1.0498049 13.427031 50-59
TACAG 2715585 1.0453783 8.6289625 120-129
CCCCT 3494165 1.0445218 9.456284 70-79
GAAGC 3489040 1.0397463 12.46093 200-209
GACAG 3479140 1.0367962 15.773721 70-79
TTTCA 1952535 1.0362808 7.8591394 100-109
GAGCC 4027755 1.0352286 18.203419 220-229
CGTGA 3250150 1.0337241 20.753075 150-159
GGAGA 3610440 1.0306734 81.98813 7
AGATC 2670270 1.0279342 12.410675 210-219
GCCAA 3301685 1.0271116 15.611706 180-189
CGAGG 4170865 1.0269251 14.913485 220-229
TGGCT 3024740 1.0267606 30.669847 40-44
GGGAG 4349760 1.0259292 14.290084 5
ATGGT 2603845 1.0248127 9.426239 120-129
AAAAA 2019900 1.0223914 5.3357635 190-199
AACTC 2531925 1.0174696 16.993818 190-199
TGAGG 3336205 1.0164677 12.038992 220-229
AGCCG 3947760 1.014668 19.260736 220-229
ACCGC 3764140 1.0099491 11.2232 220-229
TTATA 1630100 1.0030929 13.118645 110-119
AAACT 2151840 1.0026027 16.703945 130-139
GCTCT 2829350 1.0026014 16.579626 210-219
ATGAC 2600845 1.0012085 11.079848 160-169
GTAGC 3141000 0.99900866 11.287205 50-59
ACTCA 2483995 0.9982087 12.932369 190-199
TAGTT 1957265 0.9951022 14.42943 50-59
CCGTC 3442065 0.9856725 10.263623 140-149
ATGAT 2065415 0.9838877 8.955635 120-129
AAGGT 2647135 0.9761704 20.73534 15-19
CCGAG 3782270 0.9721331 18.042591 220-229
TTTAT 1479365 0.9715877 11.73363 240-247
AACTG 2522890 0.97119945 12.510322 60-69
GATAC 2508985 0.96584666 24.347883 35-39
GCAGT 3010020 0.95734984 6.4846163 50-59
GGGAC 3879180 0.9551082 9.782246 90-99
CGAGT 2994635 0.95245653 14.928595 160-169
TTTAG 1871075 0.9512819 29.619696 45-49
GTAGA 2571165 0.94815546 16.25681 170-179
ACAGG 3180375 0.94776314 15.307171 70-79
ACCAA 2490900 0.93787956 16.359264 130-139
CGGAC 3648265 0.9376907 10.521513 220-229
CATAC 2325970 0.9347054 14.007403 130-139
GCCAT 2813435 0.9341114 7.674685 60-69
GGTAT 2371040 0.9331863 9.174415 110-119
CAGAA 2584780 0.93229616 18.171268 140-149
CCAAC 2851415 0.9259829 13.405102 130-139
TAACA 1987220 0.92590165 8.797834 120-129
TATTT 1407070 0.92410725 11.422932 240-247
AGCCC 3423440 0.91853666 11.491859 80-89
TACAC 2284745 0.9181388 22.119299 35-39
AAGTT 1915805 0.912619 17.624943 140-149
TATGG 2302335 0.9061454 11.76815 35-39
TCATA 1820135 0.90511215 8.809684 110-119
GAGAT 2449600 0.90332645 12.034302 210-219
GTTAT 1768965 0.8993678 10.540038 110-119
AATCA 1928535 0.8985586 13.274091 110-119
GATGA 2425315 0.8943711 7.0675254 60-69
CCCAG 3325295 0.8922035 14.352217 80-89
AGTCT 2144830 0.88121694 15.52975 8
TGGTT 2089260 0.8776104 8.850105 50-59
GACTG 2753095 0.8756338 12.373739 90-99
GTTTC 1982550 0.8693486 5.5020967 100-109
CCCCA 3093050 0.86632574 16.566086 80-89
AGAAG 2493375 0.8615041 15.754581 140-149
GTCTG 2530530 0.858999 14.301543 1
TCCTT 1872480 0.8571317 10.233498 1
CGGAG 3476700 0.85601205 70.92827 6
CGTAT 2076285 0.85305476 7.232963 240-247
ATAGT 1778505 0.84721434 15.48674 120-129
GCCTA 2544130 0.8446973 16.510986 190-199
TTATG 1651495 0.83964425 12.163951 50-59
ACGCT 2528870 0.8396308 14.876726 190-199
TCTTC 1830925 0.8381098 45.562885 2
CATGG 2634955 0.8380588 8.599682 200-209
GGTTC 2445840 0.8302506 11.190186 160-169
GTCTA 2018845 0.8294552 9.522917 30-34
TTACC 1933685 0.8293469 9.705112 45-49
GATCA 2140985 0.82418317 9.166452 110-119
ATCCA 2042570 0.8208193 9.547904 180-189
ATATA 1421740 0.8197231 11.195017 130-139
CTATC 1909295 0.8188862 8.156113 110-119
TGGCA 2573400 0.81848097 5.765943 100-109
GGTAG 2680895 0.81680924 11.620361 120-129
GGTGC 3087060 0.8112181 11.636564 60-69
TCATC 1886780 0.8092297 10.408381 110-119
GCTTG 2380565 0.80809265 16.836449 90-99
ATATG 1681500 0.8010047 5.6060424 50-59
TGATG 2025360 0.79713464 12.340912 120-129
ACATC 1981560 0.79630214 5.5879307 110-119
ACAAA 1815255 0.79245883 12.77646 130-139
CATTA 1592835 0.792081 12.817535 110-119
TATAC 1568335 0.7798975 9.302724 130-139
CCTCC 2583605 0.7723253 10.172269 140-149
CTGAC 2299720 0.7635488 9.461827 220-229
GTTCA 1845875 0.7583894 13.641085 160-169
TCAAC 1879995 0.7554876 7.5657086 110-119
AACGC 2416845 0.75184923 15.224884 180-189
TATGA 1577805 0.7516082 5.0611396 50-59
ACGCC 2798035 0.7507354 13.651016 180-189
TGACT 1820030 0.7477708 5.23995 2
CCAGC 2777100 0.7451183 6.573157 80-89
ATCTT 1395295 0.74053335 12.495589 3
GGGAT 2417415 0.7365325 13.18903 100-109
TATTG 1445105 0.7347125 6.8739414 240-247
TTGGA 1864090 0.7336624 6.508145 60-69
CAACG 2354655 0.7325028 15.516816 180-189
GCGGA 2963465 0.72964644 8.803455 220-229
TTTAC 1364180 0.72401947 13.313226 45-49
TTTCT 1276675 0.72316736 9.782961 4
GACCG 2800250 0.71973073 8.949727 210-219
ATAAA 1332040 0.7195888 7.7127843 130-139
GGTTA 1816025 0.71474516 7.835605 50-59
GATTA 1496650 0.71294904 5.730296 50-59
GGATA 1925795 0.71016556 15.890561 35-39
GACCA 2279500 0.7091231 6.163032 170-179
GGGCA 2879785 0.7090432 13.605766 150-159
CCCCC 2927000 0.70708025 13.161339 80-89
AATAC 1509995 0.7035491 11.504051 130-139
ATGCC 2106420 0.69936967 7.978228 50-59
GAAAT 1563970 0.69805044 6.9448743 110-119
TGCCA 2100355 0.6973559 7.3498425 60-69
TATAG 1455885 0.69352996 6.6304674 120-129
TCCGG 2527885 0.69344234 11.278453 70-79
CGCCG 3127680 0.6933416 10.750175 220-229
CATCC 1984270 0.6877382 6.4321747 110-119
CAGTC 2065600 0.6858167 5.687815 210-219
CACAA 1816920 0.684111 8.379556 130-139
AAATA 1241925 0.6709073 12.763908 130-139
AGGCA 2236150 0.6663807 6.572142 80-89
CCCGT 2323360 0.6653192 10.0401945 140-149
GCCGC 3000125 0.6650653 11.8252535 220-229
CAAAC 1765510 0.66475403 13.504482 130-139
AGATG 1796680 0.66255254 6.0507436 120-129
GCGAC 2539670 0.6527555 7.2629724 70-79
CACGC 2408490 0.6462173 12.102419 190-199
GACGA 2165225 0.6452448 9.798236 220-229
ACTTC 1491475 0.6396855 29.422768 4
TACTT 1198585 0.63613224 11.359975 1
AATAA 1156905 0.6249782 7.1930747 130-139
CACCG 2322135 0.6230476 9.004369 45-49
TCAAT 1252195 0.62268835 8.060656 110-119
AAGCA 1722695 0.62135327 5.825813 120-129
AATGG 1666425 0.614519 6.725983 120-129
AGTAA 1357565 0.60592526 5.5711837 50-59
CCCTA 1738840 0.60267335 11.835294 110-119
TAGCT 1466030 0.60232764 12.406152 50-59
CCGGC 2695095 0.5974465 9.165729 70-79
TGATT 1171930 0.59582645 6.141407 50-59
TCCAC 1715025 0.59441924 10.094974 180-189
ACGAC 1910225 0.5942464 9.099289 220-229
ATTTC 1116025 0.5923147 6.793746 240-247
CACGA 1896340 0.5899269 8.656847 180-189
TTCAA 1185930 0.5897362 5.762 200-209
GGCCC 2649985 0.5874466 10.116011 70-79
GGTGA 1925710 0.5867211 6.737735 120-129
ACCTA 1453075 0.5839271 7.429113 130-139
GTTCC 1647445 0.5837846 6.859875 140-149
ACCCG 2172755 0.58296776 8.736504 140-149
CTTTG 1327390 0.5820608 7.338876 45-49
ATTAA 1002715 0.57812864 7.0532804 110-119
GGGGA 2424610 0.5718657 9.09924 4
CCGTA 1707560 0.56694084 6.1678796 230-239
TCAGG 1739345 0.5532062 5.312337 150-159
TAATA 957435 0.5520219 7.823161 130-139
CGGTT 1623355 0.5510546 11.083414 160-169
TTAGC 1339410 0.550305 9.396802 45-49
ACATT 1087670 0.5408738 6.586736 110-119
CTGTT 1227185 0.53812087 5.6968484 240-247
GGAGG 2277890 0.5372605 5.3405743 6
GAAGA 1554670 0.5371652 6.4067445 15-19
CGGCA 2089400 0.53702533 10.574642 70-79
AAGAT 1196905 0.5342174 5.778479 15-19
CTGCG 1931550 0.5298574 11.616522 25-29
CCACG 1973500 0.529506 7.596688 180-189
TATGT 1038420 0.5279479 6.927996 140-149
GCTAC 1576805 0.52352786 7.7793527 50-59
GTCCT 1474690 0.5225675 6.0544915 45-49
GGGTT 1577820 0.51307166 6.36035 100-109
AAATC 1082690 0.5044557 7.447199 110-119
AGTAT 1042580 0.49664676 5.353995 110-119
GTAAC 1286305 0.49516973 6.904473 120-129
TGGCC 1780795 0.48850268 14.872469 2
AACAT 1046810 0.4877382 6.1052094 110-119
ACCCC 1739815 0.48730102 8.654813 140-149
TATCC 1135980 0.48721555 5.3644915 110-119
CGCGG 2288885 0.48605832 6.3562136 220-229
GGTTT 1156225 0.48568156 5.8285084 100-109
TGACG 1525180 0.4850901 8.545554 220-229
CATAA 1025940 0.47801426 6.2545815 110-119
ATTCT 866615 0.45994377 7.5279517 2
CAATC 1144010 0.4597275 5.9898586 110-119
CCGGT 1666125 0.45704678 8.624053 160-169
ATGCT 1098825 0.45145917 6.8541937 50-59
CCGCG 2012970 0.44623357 6.5870414 220-229
TAAAT 767660 0.4426046 7.7221227 130-139
TGCTA 1065345 0.43770373 6.0495825 50-59
GCCTT 1218500 0.4317847 8.854115 1
TGCGC 1555930 0.42681834 8.606397 25-29
GCATG 1339675 0.4260894 8.526397 60-69
GGTCA 1327410 0.42218846 5.4486504 160-169
CACTT 975935 0.41857317 6.7483172 3
ATGGC 1286185 0.40907666 7.027462 1
CTAAC 1011430 0.40644938 5.642917 110-119
CCGGG 1865205 0.39608738 5.343977 80-89
CCTAA 985165 0.39589465 7.760345 110-119
TTTGA 778060 0.3955771 6.1035733 45-49
AGCTT 960990 0.3948288 7.161287 1
CATGC 1185875 0.39373204 7.0905266 60-69
GCGCT 1432925 0.393076 7.7111454 25-29
ACGAG 1318725 0.39298478 7.4662066 180-189
GGTAA 1027490 0.37890226 7.3050857 120-129
GAGGC 1527755 0.3761546 5.6215525 35-39
TTCTT 638275 0.36154827 19.434195 1
TGCTG 1027980 0.3489521 13.949502 1
TGGGC 1317600 0.34623912 9.1290865 1
ATGTG 865080 0.34047535 5.217083 140-149
CGAGC 1272990 0.3271886 6.280917 180-189
GCTTT 714380 0.31325573 5.1221347 45-49
CTCTT 677390 0.3100767 8.757262 1
CGGGA 1231080 0.30310908 5.071907 80-89
GCGAG 1214480 0.29902193 5.276938 240-247
GACTT 724190 0.29753804 6.593503 1
AACTT 597615 0.29718047 10.513842 3
CTTTC 646845 0.2960947 5.1826158 45-49
CGCTT 825295 0.2924495 5.798495 1
ATTGT 570825 0.29021576 5.575306 240-247
CTCGG 1057575 0.29011104 5.1251097 6
GAGGT 931930 0.28393838 6.700147 7
TTCCT 605145 0.27700636 6.1807537 4
CCCTT 732895 0.27110904 5.3090644 3
TAACT 539000 0.26803255 7.030651 2
AATCT 533095 0.26509613 7.4360175 2
TTGGC 778515 0.26427016 7.413019 1
GTCTT 595425 0.26109397 11.591249 1
TGTAA 534960 0.25483528 7.8877015 25-29
CGGGG 1251245 0.25453424 9.019779 3
CCTCG 829990 0.23767662 5.5814443 5
TGCTT 481290 0.21104576 11.02212 1
TCGGT 587545 0.19944459 6.0846324 7
TCGCA 493940 0.16399704 5.26484 7
TAGGC 480670 0.15287916 6.6873918 1
TTGCT 291700 0.1279105 5.8606887 4
Produced by FastQC (version 0.10.1)