FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_1_illumina12index_1_SL248338.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_1_illumina12index_1_SL248338.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 6429203
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 7526245 8.906051 61.64915 110-119
ATACA 6969950 7.7872343 37.86297 110-119
CACAG 10118725 7.6550326 42.653656 110-119
TCCCT 11593285 6.977265 105.84018 30-34
CAGTA 8340105 6.682609 39.455868 110-119
AGTAA 6540710 6.565615 48.37416 110-119
GGAGA 10384435 6.341589 96.96385 35-39
GTAAT 7103450 6.0283313 40.046608 110-119
GGTGA 11520440 5.9478707 103.6657 45-49
CCCTG 11313120 5.7757025 69.65926 30-34
AATAC 5020520 5.6092176 40.149765 110-119
GTAGT 9177480 5.58563 33.11799 80-89
AGACG 7986725 5.428589 103.512054 40-44
ACAGT 6675970 5.3492007 35.954193 110-119
GTGAC 8865250 5.0943227 86.127846 45-49
TAGTA 5917760 5.022097 42.915195 80-89
CCCCA 7122290 4.7870493 101.85095 60-69
GAGAC 7029905 4.778237 143.52483 40-44
CTCAG 7407920 4.737996 35.066593 140-149
ACTCC 6485360 4.6167417 119.151474 30-34
AGTAG 6199340 4.462898 34.762337 80-89
CGTGT 9087005 4.414621 23.197842 120-129
GCACA 5832635 4.4125137 34.651646 110-119
GGCCC 10166735 4.402989 58.314735 60-69
ACCAG 5802925 4.3900375 91.51475 45-49
GTGTC 8965090 4.355393 23.307682 120-129
TGACC 6713995 4.294173 146.89381 45-49
CACTC 6003920 4.2740183 98.26778 25-29
CTCCC 7356775 4.1803527 68.36583 30-34
CCCAG 6633540 4.0058117 87.81426 60-69
ATGCA 4990770 3.998914 96.335106 25-29
GACCA 5128665 3.8799453 104.9937 45-49
TGAGG 7504035 3.874247 70.449844 35-39
TACAC 4303595 3.838035 27.875916 110-119
ATGGT 6214425 3.7822444 25.93698 190-199
AGATA 3754305 3.7686005 19.914454 150-159
CCCTT 6260565 3.767838 36.83215 20-24
CCACT 5273250 3.7538755 28.189861 240-247
AGGAG 6140555 3.7499273 97.311455 35-39
GACGG 7608570 3.7088747 109.97951 40-44
ACGGT 6419555 3.6889293 98.0988 40-44
CTGAG 6334360 3.6399734 106.07646 35-39
CAGGG 7418990 3.616462 34.62877 50-59
TGTCC 6588885 3.5627685 20.220673 120-129
GAGGA 5824225 3.55675 127.68604 35-39
TGGTG 8046920 3.5123625 18.035954 190-199
TGCAC 5419100 3.465977 115.39283 25-29
TCTTG 6018030 3.4465396 18.488432 170-179
GCCCC 7136165 3.4398067 73.02859 60-69
CTTGG 7044800 3.422483 21.231897 20-24
CCGTG 7453715 3.4189453 23.299109 120-129
GGGTT 7769770 3.3913903 40.681923 50-59
CCTGA 5257260 3.3624659 85.14522 30-34
CTCTC 5549680 3.340001 13.870182 130-139
AGGCT 5791605 3.3280845 26.400408 30-34
GGATG 6428790 3.3191102 70.681816 20-24
CAGAT 4120120 3.3012955 18.236906 150-159
CTTCA 4367560 3.2930145 16.809443 200-209
TCAGG 5703055 3.2772005 21.001339 140-149
GCACT 5061125 3.2370207 89.49333 25-29
TCGCA 5052535 3.2315269 24.243391 100-109
TGTCT 5549925 3.1784549 20.108784 180-189
CCAGG 5845945 3.1717348 39.032677 50-59
CGCAC 5208475 3.1452549 24.706165 100-109
TCTCA 4140910 3.1221268 21.693214 130-139
ACAGA 3286915 3.1151989 14.900632 140-149
GGTTG 6975250 3.0445943 73.27262 15-19
CCTGG 6574100 3.0154748 28.235472 50-59
CGGAT 5246230 3.0146906 79.86129 20-24
ACCAC 3556445 2.9946108 19.80946 230-239
GACTC 4611810 2.9496455 19.784163 190-199
GATGC 5042140 2.8974123 95.88406 25-29
GCCCT 5666310 2.892829 19.81894 20-24
GGTTC 5910000 2.8711782 45.723682 50-59
TTCCC 4757320 2.8631299 47.293655 50-59
TGGCC 6161310 2.826132 42.70624 60-69
TTCTT 4183455 2.8243616 21.164783 170-179
TCTCT 4424640 2.820395 14.78243 180-189
AGGGT 5459560 2.8187077 48.87883 50-59
GTTGG 6447915 2.8144202 52.84936 15-19
CGGCC 6416870 2.779005 12.907871 120-129
GATGG 5359690 2.767146 20.74746 15-19
GCCGT 6018150 2.7604659 22.022383 120-129
TCACA 3091975 2.7574875 12.084948 140-149
CGGTG 6649240 2.7402382 69.13267 40-44
TCAAA 2447205 2.7341604 56.699036 70-79
TCCAT 3502265 2.6406069 21.017529 70-79
AGATG 3663280 2.637191 17.023424 180-189
AGGGA 4313250 2.6340246 9.278342 150-159
AGTCA 3203260 2.5666504 30.158888 70-79
CCAGT 4011830 2.5659072 34.6221 60-69
TGGAG 4957060 2.559273 18.093224 25-29
GTTCC 4714175 2.5490677 51.483738 50-59
TGTTC 4414710 2.5283146 12.984004 140-149
ACAGC 3328660 2.5182028 11.436124 120-129
GCGGA 5119775 2.4956863 87.68135 20-24
GGCTG 6035465 2.4872937 13.7108965 30-34
ACTCT 3289900 2.4804897 13.842549 200-209
TCCTC 4113100 2.4754145 16.711952 130-139
GTTCT 4260380 2.4399295 17.915293 160-169
TACCA 2723215 2.4286194 16.533792 230-239
GAGAT 3365960 2.423151 16.585262 180-189
GGCGG 6916295 2.4178734 42.20088 20-24
CAGCT 3729030 2.385033 12.545006 150-159
TGTAG 3918295 2.3847666 8.250854 210-219
CACGG 4378555 2.375598 9.916379 120-129
ACTAC 2645160 2.3590086 13.841579 230-239
CTACT 3125050 2.3561976 8.422832 230-239
TTGGG 5364665 2.341597 79.6332 15-19
CTGGA 4070995 2.3393545 14.617374 180-189
AATAA 1655675 2.3174198 9.655827 110-119
CATTT 2872255 2.2936704 17.861238 150-159
TTCAT 2842155 2.2696335 16.056807 140-149
CAGAG 3336680 2.2679467 9.684433 140-149
TCATT 2838625 2.2668147 16.65527 150-159
GATAC 2814590 2.2552238 14.972474 150-159
GTGAA 3130005 2.2532873 18.037683 190-199
CACTA 2524750 2.2516243 15.997803 240-247
TCAGC 3447300 2.2048426 14.452005 140-149
CTGGC 4803355 2.2032514 33.12745 60-69
TTCAC 2910890 2.1947272 18.313229 200-209
TTGTC 3788600 2.1697397 13.093019 170-179
ATTTG 3000480 2.1527603 15.763483 150-159
CTCGC 4180530 2.1342914 16.481094 100-109
ATGTA 2506670 2.1272814 9.3883915 70-79
ACACA 1998050 2.1076896 14.058871 110-119
TACAG 2626815 2.1047668 14.333417 160-169
TTGGA 3448395 2.098774 16.026537 170-179
AGCTC 3271180 2.0921984 12.598757 140-149
GATAT 2455755 2.0840724 8.822672 180-189
TGGGG 5617455 2.0799503 52.792183 15-19
GAACT 2580750 2.0678568 11.2232485 160-169
TCTGC 3810740 2.0605586 11.837892 210-219
CTCTG 3804345 2.0571005 14.357852 180-189
TCTCG 3782115 2.0450804 15.795824 100-109
TACTA 2160880 2.0410867 11.216987 230-239
GAATC 2547125 2.0409143 14.312229 190-199
ATATA 1721570 2.03719 10.513311 110-119
GCTGT 4182375 2.0318687 10.280374 140-149
ATATG 2391035 2.0291479 9.090315 180-189
GTTCA 2987455 2.0237327 14.842101 140-149
GTCTC 3740025 2.0223212 11.82753 180-189
ACGGC 3707680 2.011613 14.754682 120-129
GTCCT 3719390 2.0111635 14.213235 130-139
CAGGC 3700510 2.0077229 15.240444 140-149
TAGTC 2962635 2.0069191 25.097946 70-79
TGAAT 2362075 2.0045712 13.834609 190-199
AATCG 2494770 1.9989641 13.274047 190-199
TTTGC 3488460 1.997849 12.163375 150-159
GACGT 3442900 1.9784261 10.094395 60-69
ACACG 2612170 1.9761627 13.895331 120-129
AACCA 1873225 1.9760151 14.986344 70-79
TTGCA 2914575 1.9743627 14.333597 150-159
AATAT 1660485 1.9649061 13.197204 110-119
ATCTC 2587010 1.950531 7.6112566 100-109
CCAGA 2577695 1.9500816 30.90355 60-69
TGGAA 2690390 1.9368088 9.23512 170-179
CCTTC 3193495 1.9219625 13.79245 200-209
CAGCC 3176175 1.9180046 10.326652 120-129
TCGGC 4168455 1.9120291 8.048695 130-139
GAGTC 3309915 1.9020076 12.303087 210-219
AGTAT 2238785 1.8999412 12.56548 220-229
GTCAA 2369265 1.898402 38.36232 70-79
CATAG 2355965 1.8877451 8.246142 210-219
AGAAC 1962880 1.8603344 11.975402 160-169
GGGGC 5295805 1.8513651 48.131344 15-19
GTTGT 3569550 1.836705 7.167142 210-219
CGTAG 3165670 1.819119 9.691329 210-219
CTGTT 3170630 1.815827 12.788462 140-149
GGGCG 5152745 1.8013524 58.763165 20-24
GCAGA 2623210 1.7829998 14.739822 150-159
ATTGT 2446330 1.7551732 8.390223 50-59
CCTTG 3225780 1.7442566 24.051579 20-24
GGTCA 3000820 1.7243897 7.8174663 130-139
GAGAA 2023805 1.7233082 11.137713 160-169
TGACT 2541780 1.7218276 11.887764 190-199
AGTCT 2540240 1.7207844 12.978335 210-219
AACTG 2133430 1.7094364 10.999829 160-169
TGCAG 2958825 1.7002577 13.239445 150-159
GTCAC 2655230 1.6982462 8.829872 140-149
GTCCC 3309845 1.6897795 23.143194 50-59
ATAGT 1987225 1.6864552 7.3082585 220-229
TCCCA 2365965 1.6842626 11.005865 240-247
CACCA 1997335 1.6818033 12.741152 230-239
ACTGA 2094950 1.6786038 9.203003 180-189
CAAAA 1270015 1.6783607 22.527456 70-79
TGGTT 3240290 1.6672848 18.114 10-14
CTCCA 2294080 1.6330898 7.343568 150-159
TTGTA 2273445 1.6311331 9.163442 210-219
TTGGT 3159425 1.6256758 25.435492 25-29
AATAG 1618090 1.6242512 10.111869 240-247
GAACC 2142560 1.6208926 12.443355 70-79
TAGTT 2254080 1.6172392 10.004909 220-229
GGACG 3306175 1.6116287 7.394071 170-179
GTGCT 3312975 1.6094995 8.431676 220-229
GCTCA 2504530 1.6018604 11.297733 140-149
AAAAA 965800 1.5989677 8.825809 8
TGTGC 3276945 1.5919955 8.08829 220-229
GCTCC 3115980 1.5908053 6.002637 150-159
CTGGT 3273085 1.5901202 7.061169 160-169
TTCAG 2334590 1.581475 9.594659 150-159
GTCTG 3249100 1.5784677 8.953826 210-219
TCTGG 3246145 1.5770322 11.730277 180-189
TCAGA 1962755 1.572681 9.806541 130-139
GGCCG 4037710 1.5710796 13.022576 120-129
CAGAC 2072230 1.5676866 19.108202 60-69
ATAGA 1558675 1.56461 10.49339 240-247
TGCTC 2885420 1.5602158 5.0900445 150-159
ACCAT 1739480 1.5513043 12.908462 45-49
CTGTG 3179260 1.5445383 5.156631 210-219
CCCAC 2290765 1.539674 10.676559 240-247
CCACC 2262695 1.5208075 9.498119 230-239
GCTCT 2790435 1.5088551 10.235661 130-139
TGATA 1770095 1.5021883 8.274393 180-189
CCTCG 2929350 1.495525 9.637082 130-139
CAAAG 1575915 1.4935857 27.661192 70-79
CGACT 2304215 1.4737419 8.738274 190-199
ACCGT 2279185 1.457733 22.741713 45-49
CATGT 2148155 1.4551821 9.364667 70-79
TCGAC 2255725 1.4427286 9.442114 190-199
GCTGA 2502455 1.4380094 22.568554 30-34
CTCTT 2240885 1.4284055 8.505557 200-209
AATTG 1673595 1.4202936 9.37349 170-179
TATAC 1498650 1.4155688 6.9646263 110-119
TGGAC 2459035 1.4130585 10.290222 170-179
AAATA 1008665 1.4118111 5.0794463 9
GAGGG 3218910 1.4097592 8.397103 200-209
CACTG 2198855 1.4063551 7.340478 230-239
GGGAG 3210680 1.4061549 6.705836 150-159
TATGG 2296395 1.3976398 6.604321 190-199
GCGTA 2385525 1.3708167 10.130985 210-219
CTCGA 2141660 1.3697741 8.548414 190-199
CTGAT 2004285 1.3577231 7.6895604 180-189
ACGGA 1992655 1.3544106 9.287924 200-209
GTCCA 2106330 1.3471775 19.586592 70-79
CTTGA 1982840 1.343196 8.749119 200-209
GTGGA 2588135 1.3362243 22.933794 25-29
GAATT 1566280 1.3292209 10.365816 170-179
CCTCA 1860985 1.3247818 7.0480957 130-139
CCATG 2070065 1.323983 9.456613 70-79
ACGTG 2297755 1.3203807 9.559682 170-179
CCATC 1848035 1.3155631 10.602106 240-247
ACTTC 1727700 1.302636 6.5193744 220-229
AGCCG 2391065 1.2972795 9.125561 120-129
ACTAT 1370840 1.2948444 6.650849 230-239
GTGTT 2512280 1.2926887 7.4838476 160-169
GGAGG 2941425 1.2882314 17.279282 30-34
GTGGT 2950810 1.2879852 12.916333 50-59
ACTCG 2012185 1.286964 8.805642 190-199
TTGGC 2645835 1.2853914 9.2526045 60-69
AGATC 1600365 1.2823114 6.8649344 130-139
TCCAC 1798995 1.2806529 9.684915 240-247
CTGCT 2356440 1.2741836 5.664362 90-99
TGGTC 2620105 1.2728914 14.536281 50-59
GGCTC 2770385 1.2707481 8.536377 130-139
ACTGG 2181070 1.2533289 7.361131 160-169
TTGAG 2055600 1.2510862 8.425111 200-209
ATCGG 2169385 1.2466141 7.9251785 190-199
GGAGT 2410065 1.2442887 7.0035043 210-219
ATAAC 1113675 1.2442626 7.3516326 80-89
AGCTT 1830835 1.2402263 6.731587 150-159
GATCT 1830550 1.2400331 5.4707203 130-139
AAGTA 1226430 1.2311 15.724883 70-79
GGAAT 1703210 1.2261391 8.618342 170-179
TGCCC 2385785 1.2180179 8.199877 60-69
ATACC 1363455 1.2159574 8.977177 70-79
CCATA 1353965 1.207494 12.268596 70-79
TCTAC 1589020 1.1980753 7.8203325 180-189
AGAGC 1749450 1.1891037 7.5004973 140-149
TCACG 1849005 1.1825966 9.319287 200-209
ATAAA 839955 1.1756706 5.492091 7
GTCTA 1724410 1.1681329 7.868066 180-189
GCATA 1449710 1.1615975 7.5784793 210-219
AAAGT 1154120 1.1585146 20.220436 70-79
TTCCA 1514130 1.1416103 7.449736 230-239
AAAAG 957755 1.1371764 15.554666 70-79
TACTG 1677535 1.1363792 6.90753 180-189
GCTTC 2099120 1.1350446 5.9115753 150-159
GAGCT 1961635 1.1272328 6.6876335 140-149
ACTGT 1661990 1.1258489 5.5783052 230-239
TATCA 1186505 1.1207284 7.399622 70-79
AGTTC 1644210 1.1138046 5.5128236 160-169
AGGTT 1810605 1.1019765 23.573404 10-14
CTCGG 2388000 1.095352 8.346516 130-139
CGGCT 2385275 1.094102 7.9700756 130-139
CTACC 1535970 1.093413 8.799104 230-239
TATGT 1515885 1.087605 7.793458 220-229
ATCCA 1216730 1.085105 15.340951 240-247
CAATA 967035 1.080428 6.436165 100-109
TCTGT 1883485 1.0786761 6.1319723 210-219
ATGGG 2086745 1.0773623 32.64205 15-19
CTGCG 2343210 1.0748073 6.980876 210-219
AGAGA 1254040 1.0678387 14.252894 35-39
ATATC 1118205 1.0562147 7.862791 70-79
GAAGA 1235610 1.0521452 19.02125 35-39
GTCCG 2251500 1.0327408 5.1748204 130-139
AACTT 1082725 1.0227016 5.7761016 200-209
GCGGT 2480470 1.022234 5.901292 130-139
ATACG 1273495 1.020403 5.42567 120-129
TAGAT 1199425 1.0178901 7.9485755 240-247
CTGAA 1266050 1.0144378 15.519651 35-39
CTGCC 1985865 1.0138463 5.501401 200-209
TGCGT 2081505 1.0112305 8.138681 210-219
TCCCC 1778565 1.0106369 5.3942156 80-89
TATAA 849910 1.0057262 6.6953464 240-247
GGGGT 2700865 1.0000373 7.6122313 10-14
CGAAC 1316555 0.99600214 13.062809 70-79
AAAAT 709635 0.993264 5.440927 8
CATGG 1725595 0.991595 5.254574 190-199
CGTGG 2402500 0.99010164 8.899586 50-59
ACTAA 876655 0.9794502 13.030378 240-247
ACGTC 1515695 0.9694163 13.452909 70-79
CAGCG 1777680 0.96448565 6.4320703 160-169
CGGAG 1971415 0.96098626 6.5857806 210-219
CCCAA 1136510 0.9569683 10.121679 240-247
AGCTG 1661225 0.9546054 5.1356606 90-99
CTTCT 1492470 0.951344 5.249439 210-219
ACCCA 1120085 0.94313806 12.724308 240-247
TGTAA 1109935 0.9419446 6.8318353 230-239
GAGTT 1540205 0.9374048 5.3203254 160-169
CCATT 1234220 0.9305663 6.3129916 50-59
ACAAT 832710 0.9303523 7.4570775 100-109
AAGAG 1074530 0.91498256 14.229965 35-39
TGAAG 1260740 0.90760535 10.324855 35-39
TAGGC 1577665 0.9065885 5.5092635 160-169
CATCA 997390 0.8894931 5.3187876 230-239
TCGAA 1108630 0.8883031 16.842604 70-79
GGTGG 2397160 0.8875859 22.696749 25-29
TGCAT 1307360 0.885619 5.86126 210-219
CGGTC 1914050 0.87795573 5.701189 130-139
ATCAA 781770 0.873439 14.016203 70-79
CGTCC 1701580 0.86870986 10.947903 70-79
CCAAT 969415 0.8645444 10.709809 240-247
GAGGC 1764420 0.8600845 14.624747 30-34
GACCG 1566050 0.8496651 20.693403 45-49
CCCCC 1581700 0.84858686 5.0757256 80-89
TGAAC 1055100 0.8454115 6.549759 190-199
AGCGT 1458100 0.83788174 6.5529566 160-169
TTCTG 1451075 0.83103395 5.113619 210-219
GTATG 1361930 0.8289026 5.4496546 220-229
GGTCC 1803870 0.8274173 14.563942 50-59
GGGCC 2117190 0.8238021 17.17555 15-19
CCAGC 1356565 0.8191921 5.583717 80-89
AACCG 1081075 0.81785643 5.780294 190-199
CTTCC 1354155 0.81498027 5.359994 230-239
GGTGC 1924440 0.7930868 5.0321918 220-229
TAACC 882540 0.7870674 5.8620715 80-89
CATAC 873140 0.7786844 10.768693 70-79
TGGGC 1887440 0.77783865 18.672342 15-19
CTAAT 819195 0.77378106 9.87815 240-247
AATCC 835875 0.7454506 9.599728 240-247
ATGAT 875685 0.7431486 6.7676415 240-247
AAAGA 622670 0.73931813 5.845293 8
AAAGG 862045 0.73404765 9.092527 10-14
GTAGC 1257200 0.7224367 5.672103 80-89
TGAGA 1001850 0.72123075 5.7440267 240-247
CCTTT 1127720 0.7188416 5.5941463 50-59
AGCCA 947540 0.71683437 5.759387 80-89
AAAGC 749150 0.7100127 10.503423 70-79
GTCGA 1217825 0.6998103 11.628106 70-79
TAGGT 1125350 0.68491435 5.5286765 10-14
AAGGT 949715 0.6836987 10.050743 10-14
TATGA 801025 0.6797885 5.9834085 240-247
CATTG 997250 0.67554736 5.782801 50-59
CCGTA 1037605 0.6636368 5.6856937 80-89
CAATC 733960 0.65456074 9.400373 240-247
GAGGT 1226685 0.6333233 6.9323297 10-14
AGGAT 874915 0.6298504 6.2489038 10-14
CGATG 1073455 0.61684954 14.307419 15-19
GGAAG 1001460 0.6115737 6.2604284 30-34
AGAAG 713965 0.6079547 5.609198 9
ATAGG 831410 0.59853107 5.3124037 10-14
CATCC 811125 0.5774166 7.2476416 240-247
AATGG 793840 0.5714845 6.677673 10-14
TAAAA 401755 0.5623296 6.1536965 8
AAGCA 575695 0.54561937 7.1470504 70-79
GGGTC 1321035 0.5444157 5.542794 70-79
AAGAA 453240 0.5381478 5.399979 9
AAATG 524020 0.52601534 5.362553 9
ACGAT 643360 0.5154999 6.471791 10-14
GAAAA 430335 0.51095194 6.2341957 8
GAAGG 832830 0.5085944 5.5820756 10-14
AAGGG 832225 0.5082249 6.3555284 10-14
TTGCC 922950 0.4990612 5.7890534 60-69
GGTCG 1154220 0.47566915 5.6319017 70-79
ATAAG 460800 0.4625546 5.689886 9
TACCG 722425 0.46205235 5.7350807 70-79
TAAGG 595450 0.42866376 5.9778767 10-14
CGGTT 876125 0.42563635 8.089709 10-14
GAAGC 620080 0.42146927 5.065517 30-34
AAGCT 508085 0.40710917 8.481959 30-34
TAAAG 384955 0.38642082 5.620452 9
Produced by FastQC (version 0.10.1)