FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_11_SL248340.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_11_SL248340.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12502379
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 14294190 8.4909115 60.18184 110-119
ATACA 13050620 7.3383393 37.401943 110-119
CACAG 18965240 7.3114395 41.40636 110-119
TCCCT 22351090 7.064177 107.17796 30-34
CAGTA 16104945 6.558914 38.70275 60-69
AGTAA 12364200 6.214163 46.71147 110-119
GGAGA 19728010 6.0761414 93.63795 35-39
CCCTG 22387825 5.9868264 70.38919 30-34
GTAAT 13523250 5.818109 39.11235 110-119
GGTGA 22019525 5.8054605 100.46024 45-49
AATAC 9525010 5.355896 39.39891 110-119
GTAGT 16818825 5.2408586 27.723112 80-89
AGACG 14934240 5.1460934 100.04726 40-44
ACAGT 12483465 5.0840263 34.913666 110-119
GTGAC 16967525 5.0049205 83.54916 45-49
GAGAC 13737355 4.7336664 139.23166 40-44
CCCCA 13479185 4.711013 100.743126 60-69
ACTCC 12733255 4.701302 120.574005 30-34
CTCAG 14015545 4.625287 34.665775 140-149
ACCAG 11773455 4.5388775 93.651146 45-49
TAGTA 10312685 4.436827 35.121414 80-89
GGCCC 19305060 4.3679514 59.19742 60-69
GCACA 11260855 4.3412614 33.59919 110-119
GTGTC 17166345 4.334516 23.845472 120-129
CACTC 11691285 4.3165917 98.829185 25-29
CGTGT 16988875 4.2897053 23.391987 120-129
TGACC 12877635 4.249764 143.14235 45-49
CTCCC 14016580 4.1935015 69.73386 30-34
AGTAG 11391155 4.1465836 28.337063 80-89
ATGCA 9761895 3.9756377 95.12669 25-29
GACCA 10258040 3.954658 105.82042 45-49
CCCAG 12653480 3.9528525 86.125946 60-69
TGAGG 14337145 3.7799964 68.57279 35-39
CCACT 10064440 3.715937 27.016623 240-247
TACAC 8065910 3.6751585 27.504137 110-119
AGGAG 11906520 3.667156 93.95423 35-39
CAGGG 14664515 3.659892 35.456345 50-59
ATGGT 11712230 3.649609 25.204258 190-199
ACGGT 12292565 3.6259449 96.09653 40-44
GACGG 14519705 3.6237512 107.39763 40-44
CTGAG 12242620 3.611213 104.32224 35-39
TGTCC 12548495 3.544904 20.488743 120-129
CCCTT 11168950 3.5300043 34.985462 20-24
TGCAC 10670225 3.5212939 115.4213 25-29
AGATA 6998370 3.5173333 18.82591 150-159
GGGTT 15398650 3.4753256 42.51557 50-59
GAGGA 11225790 3.4574945 122.648994 35-39
GCCCC 13544030 3.4285028 72.96258 60-69
TGGTG 15141000 3.417176 17.72384 190-199
CCTGA 10206460 3.3682463 84.66183 30-34
GGATG 12588355 3.318927 70.038765 20-24
TCTTG 11038960 3.2943456 17.876556 170-179
CCAGG 11787885 3.2914438 40.29593 50-59
CCGTG 13724980 3.280548 23.465694 120-129
GCACT 9899170 3.266837 89.58604 25-29
CTTGG 12893580 3.2556403 20.014406 20-24
CTTCA 8329890 3.2489731 16.372036 200-209
AGGCT 10993740 3.24283 24.454306 30-34
CTCTC 10234880 3.2347865 13.714673 100-109
CAGAT 7851260 3.1975107 17.591356 150-159
TCAGG 10786415 3.1816752 20.528658 140-149
CCTGG 13306055 3.1804166 29.84385 50-59
AAAAA 3867895 3.1354592 18.67769 130-139
TCGCA 9462870 3.1228535 24.116718 100-109
TGTCT 10438920 3.115276 19.485754 180-189
TCTCA 7818255 3.049416 21.46478 130-139
GGTTG 13491365 3.04487 73.99162 15-19
CGCAC 9737915 3.0420516 24.638271 100-109
TTCCC 9592690 3.031819 50.287247 50-59
CGGAT 10222265 3.0152674 79.584015 20-24
ACAGA 6245670 2.9714441 14.351017 140-149
GGTTC 11653180 2.942438 48.025986 50-59
GATGC 9879535 2.914172 95.90857 25-29
TGGCC 12187695 2.9131064 43.486126 60-69
AGGGT 10997150 2.8994048 49.977695 50-59
ACCAC 6677385 2.8800564 19.137665 230-239
GACTC 8620650 2.8449113 19.697533 190-199
GTTGG 12548675 2.8321135 53.162678 15-19
GCCCT 10571660 2.8270137 18.72565 20-24
TCTCT 8374465 2.79607 14.641981 180-189
TCAAA 4901755 2.7562478 56.774094 70-79
CGGTG 12898545 2.7556589 68.39951 40-44
AGTCA 6756395 2.7516153 31.51286 70-79
GCCGT 11497910 2.748233 22.11975 120-129
CCAGT 8296065 2.7377942 36.955223 60-69
TTCTT 7677350 2.7078862 20.095387 170-179
GATGG 10256495 2.7041306 19.124825 15-19
CGGCC 11924935 2.6981287 12.773317 120-129
GTTCC 9457525 2.6717162 54.427174 50-59
TCACA 5706270 2.6000102 11.583584 140-149
AGATG 7056880 2.5688303 16.742842 180-189
TCCAT 6546565 2.5534089 17.20182 70-79
AGGGA 8205735 2.5273306 9.136137 150-159
GCGGA 10030140 2.5032692 87.11335 20-24
GGCTG 11638475 2.4864564 12.795886 30-34
TCCTC 7768945 2.4554152 16.600334 130-139
TGGAG 9255020 2.4400914 16.672625 25-29
GGCGG 13460330 2.4331133 42.4713 20-24
TGTTC 8149675 2.4320993 12.781705 140-149
ACTCT 6199575 2.4180694 13.589264 200-209
CACGG 8609405 2.4039402 9.688622 120-129
TACCA 5205280 2.3717384 16.02045 230-239
TTGGG 10496405 2.3689363 79.93607 15-19
ACAGC 6141525 2.367668 10.849532 120-129
CTGGC 9827545 2.3489828 34.982906 60-69
GAGAT 6427515 2.33973 16.267958 180-189
GTTCT 7833425 2.3377209 17.631145 160-169
CAGCT 7072760 2.3340902 12.277618 150-159
TGTAG 7435260 2.3168766 8.175686 210-219
AATAA 3335980 2.3149097 9.653889 110-119
CTGGA 7720545 2.2773337 14.114133 180-189
TCAGC 6760770 2.2311301 14.732985 140-149
CATTT 5357555 2.2075043 17.085524 150-159
TCATT 5345860 2.2026856 15.910948 150-159
CTACT 5636020 2.1982617 7.490647 180-189
ACTAC 4818125 2.195335 12.098249 230-239
CAGAG 6334390 2.1827266 9.435983 140-149
TTCAT 5259430 2.1670735 15.748008 140-149
TTCAC 5555110 2.1667037 18.014225 200-209
CACTA 4719290 2.1503015 14.543152 240-247
GTGAA 5878685 2.1399462 17.178165 190-199
TCTGC 7554320 2.134068 11.8383665 210-219
TTGTC 7143355 2.1317842 13.147394 170-179
TGGGG 11057245 2.111454 52.93438 15-19
GATAC 5169645 2.105394 14.294002 150-159
CTCGC 7812055 2.089056 16.662695 100-109
ATTTG 5641985 2.0778625 14.992757 150-159
TAGTC 5955630 2.0762734 26.291208 70-79
TCTCG 7318650 2.067492 16.800657 100-109
GAACT 5069430 2.0645804 10.947417 160-169
TTGGA 6615895 2.061557 15.760791 170-179
ATCTC 5267000 2.0543296 8.714068 100-109
ATGTA 4772860 2.053428 7.6939855 70-79
ACGGC 7307980 2.0405533 14.318506 120-129
CTCTG 7220465 2.039755 14.190269 180-189
GCTGT 8064460 2.036283 9.792183 140-149
AGCTC 6160440 2.0330143 12.638581 140-149
ATATA 3415215 2.0286767 10.171273 110-119
GTCTC 7153670 2.0208857 11.559695 180-189
ACACA 3795450 2.0202346 13.611532 110-119
GTCCT 7109940 2.008532 14.013166 130-139
GATAT 4647605 1.9995395 8.220454 180-189
ACACG 5183565 1.998357 13.305313 120-129
GAATC 4891795 1.9922366 13.911109 190-199
GTCAA 4886790 1.9901984 40.290955 70-79
TACAG 4884810 1.989392 13.221486 160-169
CAGGC 7060830 1.9715434 14.923415 140-149
GTTCA 5643100 1.967318 14.779046 140-149
AACCA 3663180 1.9498302 15.082069 70-79
TGAAT 4503525 1.937552 13.333115 190-199
AATCG 4754420 1.9362893 13.104806 190-199
AATAT 3240105 1.9246593 12.686675 110-119
TTGCA 5508620 1.9204352 13.797689 150-159
TTTGC 6409470 1.9127716 11.780627 150-159
CCTTC 6050345 1.9122425 13.601411 200-209
TGGAA 5242200 1.9082543 8.973372 170-179
TACTA 3935435 1.8942771 9.685907 230-239
GAGTC 6385310 1.8834785 11.849396 210-219
ATATG 4362235 1.8767649 8.481801 180-189
GGGGC 10367510 1.8740497 48.256523 15-19
CAGCC 5948230 1.8581825 9.962601 120-129
CATAG 4544765 1.850905 8.255721 210-219
TCGGC 7706600 1.8420339 7.689871 130-139
GGGCG 10092710 1.8243763 59.341007 20-24
GACGT 6068780 1.7901114 9.830566 170-179
GTTGT 6702425 1.7878162 7.1166778 210-219
AGTAT 4127550 1.7757962 11.404859 220-229
CTCCA 4804320 1.7738246 7.975444 150-159
CCAGA 4584990 1.7675958 26.131048 60-69
CTGTT 5888735 1.7573692 12.451303 140-149
AGAAC 3691215 1.7561349 11.340036 160-169
CGTAG 5929885 1.7491417 9.543285 210-219
GTCAC 5290235 1.7458369 8.7857 130-139
CACCA 3974620 1.7143133 13.141202 230-239
ATTGT 4641410 1.709365 7.555409 50-59
GGTCA 5731885 1.6907375 7.7279987 130-139
TGACT 4847990 1.6901239 11.823338 190-199
GCAGA 4898205 1.6878409 14.031255 150-159
TCCCA 4561030 1.6839983 10.882457 240-247
AGTCT 4825900 1.6824229 12.5887375 210-219
TGCAG 5689960 1.6783708 13.153709 150-159
CCTTG 5932700 1.6759661 22.721382 20-24
ATAGT 3849965 1.6563709 7.25331 220-229
TCAGA 4063990 1.6551043 10.28899 130-139
TTCAG 4722950 1.646532 9.394628 150-159
TGGTT 6171590 1.6462203 17.883188 10-14
AACTG 4031660 1.6419374 10.753984 160-169
GTCTG 6484655 1.6373808 8.7632885 210-219
CAAAA 2486575 1.6333683 20.5575 70-79
TGTGC 6440185 1.626152 8.1985855 220-229
CTGTG 6434175 1.6246349 5.395578 210-219
GCTCC 6074940 1.6245263 6.4731703 150-159
GAGAA 3819090 1.6240464 10.3682785 160-169
TGCTC 5739685 1.62144 5.4460063 150-159
TAGTT 4374785 1.6111709 9.948533 220-229
TTGTA 4339955 1.5983436 9.113568 210-219
GTGCT 6321330 1.5961411 8.18418 220-229
AATAG 3171370 1.5939091 9.2556715 240-247
ACTGA 3905295 1.5904738 8.904326 180-189
CTGGT 6284285 1.5867875 7.0924964 160-169
TTGGT 5943510 1.5853819 23.771849 25-29
GTCCC 5911345 1.5807786 19.976345 50-59
ATAGA 3136790 1.5765294 10.100278 240-247
CAAAG 3297110 1.5686353 28.612967 70-79
TCTGG 6209355 1.5678676 11.606777 180-189
GGCCG 7697495 1.556704 13.165837 120-129
GCTCA 4703680 1.5522672 11.355369 140-149
GAACC 4002510 1.5430392 12.563219 70-79
GGACG 6142355 1.5329766 7.294029 170-179
CCCAC 4347985 1.5196329 10.217333 240-247
ACCAT 3315885 1.5108528 11.444936 45-49
CCACC 4302365 1.5036885 9.553652 230-239
GCTCT 5145180 1.4534945 9.950576 130-139
CATGT 4141220 1.4437271 7.6252165 70-79
CCTCG 5380270 1.4387615 9.267158 130-139
CACTG 4347845 1.4348376 7.3784585 230-239
GCTGA 4851085 1.4309273 20.943607 30-34
CGACT 4334495 1.4304318 8.758379 190-199
CAGAC 3701695 1.4270697 15.265991 60-69
TATAC 2930865 1.4107387 6.5692687 110-119
TGGAC 4757310 1.4032664 10.444977 170-179
CTCTT 4196540 1.4011426 8.245108 200-209
TCGAC 4230420 1.3960862 9.376616 190-199
TGATA 3240970 1.3943628 7.7430835 180-189
GGGAG 6235910 1.3910745 6.651908 150-159
GAGGG 6225105 1.3886642 8.370546 200-209
CTGCT 4893730 1.382461 5.7188773 90-99
AGATC 3358500 1.3677856 7.087727 130-139
CCTCA 3694480 1.3640555 7.3700557 130-139
AATTG 3168295 1.3630959 9.098416 170-179
GCGTA 4600670 1.3570622 10.031579 210-219
CTTGA 3882010 1.3533604 8.392161 200-209
CCATC 3626200 1.3388456 10.920081 240-247
ACTTC 3416525 1.3325742 6.5720916 220-229
GTAGG 5037080 1.328029 5.1703434 160-169
CTGAT 3794040 1.322692 7.3928185 180-189
GTGGA 5014260 1.3220125 21.129429 25-29
CTCGA 3970680 1.3103691 8.428887 190-199
CCATG 3966765 1.3090769 7.718699 70-79
ACGGA 3789795 1.305901 9.113291 200-209
TCCAC 3534715 1.305068 9.650366 240-247
TATGG 4132920 1.2878455 6.1811333 180-189
ACGTG 4314580 1.2726742 9.505422 170-179
GAATT 2951965 1.2700243 10.062627 170-179
ACCGT 3827380 1.2630782 18.28378 45-49
GTGTT 4729305 1.2615027 7.410088 160-169
ATCGG 4260270 1.2566543 7.754395 190-199
GGAGG 5624265 1.254632 15.941539 30-34
GGAAT 3446440 1.2545656 8.358183 170-179
ACTAT 2593150 1.2481835 6.133828 230-239
GGAGT 4698980 1.2388887 6.801966 210-219
AGCCG 4433245 1.2378621 8.857596 120-129
ACTCG 3745435 1.2360356 8.681233 190-199
GGCTC 5154240 1.231968 8.25321 130-139
AAGTA 2444905 1.2287927 15.694548 70-79
TCACG 3715680 1.2262161 9.425351 200-209
GATCT 3515715 1.2256614 5.6480794 130-139
GTGGT 5423770 1.224092 10.499627 50-59
GTCCA 3709115 1.2240496 16.084162 70-79
AAAGT 2430970 1.2217891 20.990677 70-79
ATAAC 2172840 1.221784 7.4505615 80-89
ACTGG 4113535 1.2133718 7.2500544 160-169
ATAAA 1739305 1.206942 5.2038755 7
TGGTC 4759800 1.2018534 11.887451 50-59
TTGAG 3854255 1.2010117 8.072097 200-209
TGCCC 4441680 1.187769 6.755345 60-69
AGCTT 3403450 1.1865231 6.243696 150-159
TTGGC 4697740 1.1861832 8.101074 60-69
GCATA 2903630 1.1825348 7.9889655 210-219
AGAGC 3395255 1.169949 7.077391 140-149
ACTGT 3345175 1.1662071 5.602572 230-239
TTCCA 2972805 1.1595068 7.3547235 230-239
ATACC 2536875 1.155904 7.2489142 70-79
TCTGT 3839670 1.1458688 6.5477 210-219
CGGCT 4763095 1.1384761 7.6304812 130-139
TATCA 2354275 1.1332036 6.745497 70-79
AAAAG 1923000 1.1290464 13.695034 70-79
TCTAC 2860240 1.1156021 7.2823753 180-189
CCATA 2435895 1.1098934 10.118984 70-79
ATCCA 2430980 1.107654 15.506753 240-247
CAATA 1964135 1.1044295 6.529638 100-109
GAAGA 2595160 1.1035771 17.904497 35-39
GTCTA 3147215 1.0971936 7.3822703 180-189
AGGTT 3521060 1.0971859 23.09612 10-14
ATATC 2267705 1.0915343 7.24552 70-79
GCTTC 3857925 1.0898497 5.6714478 150-159
AGTTC 3123745 1.0890113 5.240605 160-169
TACTG 3084830 1.0754447 6.516794 180-189
GAGCT 3643360 1.074684 6.412688 140-149
CTGAA 2632095 1.0719495 14.225195 35-39
CTTCT 3184680 1.0633024 5.527593 210-219
TATGT 2882900 1.0617309 7.2354207 220-229
CTCGG 4433885 1.0597886 8.062381 130-139
CTGCC 3961975 1.0594891 5.863421 200-209
AGGGG 4719940 1.0528998 5.0024524 60-69
CTACC 2836005 1.0470941 8.365405 230-239
AGAGA 2458150 1.0453143 12.615931 35-39
CTGCG 4368330 1.0441196 6.6780834 210-219
AACTT 2160620 1.0399901 5.961498 200-209
ATGGG 3935405 1.0375718 30.2857 15-19
GGGGT 5430865 1.0370597 7.911334 10-14
GTCCG 4335670 1.0363129 5.038854 130-139
TAGAT 2407600 1.0358219 7.765411 240-247
CATGG 3506005 1.0341682 5.315351 190-199
AAAAT 1489615 1.0336766 5.368016 8
GCGGT 4819165 1.0295714 5.9107924 130-139
TGCGT 3940210 0.99490637 8.063666 210-219
ATACG 2407045 0.98029524 5.0140495 120-129
TATAA 1642025 0.9753815 6.322883 240-247
TCCCC 3228545 0.965921 5.0479393 80-89
CAGCG 3458180 0.9656021 6.1517854 160-169
AAGAG 2266550 0.96383744 13.330223 35-39
ACCCA 2224430 0.95943004 12.721638 240-247
CGAAC 2486905 0.9587463 13.054252 70-79
TGTAA 2213375 0.95226055 6.9315653 230-239
AGCTG 3227630 0.9520559 5.103829 90-99
TAGGC 3217555 0.94908404 5.8145633 160-169
ACAAT 1679330 0.94428426 7.528958 100-109
CGTGG 4396465 0.93926543 7.280718 50-59
TTCTG 3146210 0.93892026 5.4615946 210-219
CGGAG 3759115 0.93818 6.3672557 210-219
CCATT 2381520 0.92888314 5.786445 50-59
ACTAA 1646685 0.92592794 11.785793 240-247
CCCAA 2144165 0.92481065 9.801434 240-247
TGCAT 2615095 0.9116839 6.158024 210-219
ACGTC 2713490 0.8954821 11.033048 70-79
TCGAA 2183650 0.8893153 16.628372 70-79
TGAAG 2428080 0.8838644 9.321083 35-39
TGAAC 2168025 0.8829517 6.179803 190-199
GGTGG 4594515 0.8773531 21.13445 25-29
CCAAT 1911230 0.8708346 10.457362 240-247
CATCA 1899780 0.86561745 5.540316 230-239
GTATG 2767885 0.8624915 5.139634 220-229
CGGTC 3567020 0.85259014 5.661052 130-139
CCAGC 2723555 0.85081816 5.6255302 80-89
GAGGC 3400255 0.84861773 13.527413 30-34
ATCAA 1490485 0.838097 12.453422 70-79
AGCGT 2810235 0.82893664 6.3091006 160-169
CTTCC 2620750 0.82830155 5.2096586 230-239
AAAGA 1410045 0.8278764 5.2547445 8
GGGCC 3967610 0.80239016 16.210604 15-19
TAACC 1734045 0.7901018 6.0512295 80-89
GACCG 2807335 0.78387135 16.923376 45-49
CGTCC 2927100 0.78274864 9.122348 70-79
AACCG 2007070 0.7737614 5.4792433 190-199
TGAGA 2124700 0.7734287 5.640131 240-247
TGGGC 3528290 0.7537876 17.594378 15-19
AAAGG 1762650 0.74955696 8.562068 10-14
GGTCC 3127430 0.74751925 12.135949 50-59
AATCC 1624020 0.7399699 9.462032 240-247
ATGAT 1718835 0.73949456 6.8425956 240-247
CTAAT 1529920 0.7364097 9.143914 240-247
AGCCA 1909370 0.7360963 5.875356 80-89
GTCGA 2456440 0.7245775 11.933502 70-79
GTAGC 2402890 0.70878184 5.4689803 80-89
CATAC 1551145 0.7067651 8.638513 70-79
AAGGT 1902085 0.6923929 9.90406 10-14
GGAAG 2222410 0.6844925 5.841918 30-34
AAAGC 1414750 0.6730825 9.482056 70-79
CATTG 1923665 0.6706351 5.267012 50-59
CAATC 1443640 0.6577814 9.204266 240-247
GAAAA 1116805 0.65570706 5.849402 8
TAGGT 2099020 0.65406865 5.429297 10-14
AGGAT 1765850 0.6428009 5.7611475 10-14
TATGA 1482905 0.63799036 5.5010195 240-247
GAGGT 2401425 0.63313705 6.7771935 10-14
AGAAG 1456750 0.6194746 5.2938757 9
ATAGG 1694965 0.6169975 5.1247864 10-14
CCGTA 1831495 0.6044139 5.089212 80-89
CGATG 2039510 0.6015954 13.174662 15-19
TAAAA 851045 0.5905588 5.568578 8
AATGG 1621290 0.5901785 6.627491 10-14
GGGTC 2759790 0.58960444 5.6868634 70-79
AAGAA 999535 0.58685464 5.345552 9
CATCC 1580650 0.58359885 7.3977356 240-247
AAATG 1130050 0.5679555 5.621804 9
GAAGG 1773680 0.5462857 5.517227 10-14
AAGGG 1698530 0.52313983 6.3532395 10-14
AAGCA 1088970 0.5180891 6.2405457 70-79
TGATC 1477975 0.515257 5.4866056 240-247
GGTCG 2330290 0.49784565 5.7997303 70-79
GATCC 1502220 0.49574944 5.5592284 240-247
ATAAG 937395 0.4711284 5.537388 9
ACGAT 1114490 0.45388815 6.1514177 10-14
CGGTT 1756995 0.4436427 8.124398 10-14
TAAGG 1216680 0.44289324 5.927925 10-14
TAAAG 813450 0.4088345 5.6633925 9
AAGCT 974510 0.39687976 7.673235 30-34
TGGGT 1751470 0.3952897 5.046668 10-14
Produced by FastQC (version 0.10.1)