FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_13_SL248342.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_13_SL248342.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 11123587
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 46895 0.4215816354922203 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 37313 0.33544035750338447 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 37043 0.33301308292010484 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 35655 0.3205350935808746 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 35410 0.3183325666441949 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 34321 0.30854255915830026 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 34182 0.30729296224320446 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 33304 0.2993998248946136 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 31469 0.2829033476341759 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 31063 0.27925344585339246 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 28919 0.25997908768097916 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 28803 0.2589362586007553 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 28546 0.25662585279370764 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 28233 0.25381201225827604 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26818 0.24109129546071784 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26740 0.24039008280332597 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26132 0.2349242200380147 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26109 0.23471745220314272 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25936 0.23316219848867098 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25558 0.22976401407207944 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 25397 0.22831663922797565 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24801 0.2229586553330324 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24519 0.22042350187938478 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24468 0.21996501668032084 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24215 0.21769057049672913 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23789 0.21386087059866568 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23222 0.20876359397377842 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22435 0.20168853805881143 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22301 0.20048389067303557 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21923 0.1970857062564441 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21882 0.1967171201160201 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21097 0.18966004401278114 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21016 0.18893186163779724 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20917 0.18804186095726136 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20629 0.18545276806842972 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20042 0.1801756933262625 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19775 0.17777538846057483 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 19478 0.1751053864189672 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19397 0.1743772040439833 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18861 0.16955861450087997 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 18601 0.1672212389762403 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18510 0.16640315754261642 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18445 0.1658188136614565 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 18376 0.1651985101568406 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18107 0.16278022547942494 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18037 0.16215093206894501 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 17733 0.15941800068628942 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 17146 0.15414092594412215 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17004 0.15286435931143433 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16853 0.15150688352597055 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16851 0.15148890371424253 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15954 0.1434249581542357 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15761 0.14168990632248393 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 15704 0.141177481688236 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15536 0.13966717750308422 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 15203 0.13667353885037264 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15087 0.1356307097701488 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14886 0.13382373869148503 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 14498 0.13033565521625354 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14063 0.12642504616541408 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13937 0.12529231802655025 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 13391 0.12038382942480695 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13234 0.11897241420415915 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13114 0.11789362550047928 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12675 0.11394705682618385 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 12319 0.11074665033860032 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11334 0.10189159306256157 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 22633655 7.683994 66.54993 35-39
TGGAT 17301970 7.289086 79.48042 35-39
TATTA 11071550 7.2730556 126.940605 240-247
TCACC 17486255 6.422725 74.389534 160-169
TCTCC 15525615 6.0928593 86.19322 20-24
CTGTG 16310470 5.9162774 86.15665 25-29
TCCCT 14722210 5.7775717 72.19933 15-19
AGTGG 17414040 5.6837726 39.930428 100-109
CTGAG 16649790 5.6525064 49.745106 15-19
TGTAT 10158820 5.5240955 60.240135 240-247
TTACT 9336180 5.2805877 110.980705 240-247
ATTAC 9376840 4.9638634 104.099594 240-247
TGGGT 14049690 4.899522 44.81311 100-109
CCTGA 13811555 4.8771935 51.22466 15-19
CTCTG 12608045 4.75691 73.45262 35-39
TTCAC 10282025 4.6864767 74.312904 40-44
GACAC 14033720 4.6382103 46.947384 230-239
ACACG 13873865 4.585378 49.360764 230-239
CTCCA 12479975 4.5839124 40.677204 80-89
TGAGA 11312620 4.460576 93.7677 15-19
ATTCA 8273540 4.3798037 111.00679 40-44
TCTGG 11910865 4.320414 135.11073 35-39
AAGGG 14065715 4.29684 39.687637 90-99
TACTG 9784515 4.2875776 78.328384 240-247
AGCCT 11841105 4.18138 50.72485 30-34
GAAGG 13621195 4.1610465 31.512384 90-99
CCCTG 13678305 4.158764 84.38673 10-14
CAGCC 14573775 4.1471934 54.722366 30-34
TGGAG 12698080 4.1445293 38.23583 90-99
CCAGG 15080335 4.125704 52.441887 80-89
ACCAT 9618855 4.103377 67.030205 160-169
GAGAC 12683695 4.0302157 86.750275 8
TCAGT 9119185 3.9960296 99.4986 7
TCCAG 11266545 3.978489 40.239597 80-89
CACCA 11524340 3.9617662 57.13166 160-169
GTATT 7271190 3.953879 69.1302 240-247
ACTAC 9182595 3.9172697 34.162437 130-139
GGGTC 13807540 3.8802347 124.17787 8
TACTA 7329510 3.8800578 28.144796 130-139
CTGGG 13576460 3.815296 131.16731 3
CACCT 10375620 3.8109798 43.611145 40-44
ACTGT 8596020 3.766779 67.47623 240-247
TGAAG 9498435 3.7452414 39.2308 150-159
GATTC 8543525 3.7437751 58.462696 40-44
GACTC 10600935 3.743446 51.584885 20-24
GGACA 11627710 3.694679 28.57193 220-229
AGACT 8974250 3.6806204 68.78213 15-19
CAAGA 9392855 3.6055436 43.683304 180-189
ACTGG 10513960 3.569428 45.016983 60-69
GCTGG 12540240 3.524095 33.188877 2
CCTGT 9250240 3.490038 61.098553 25-29
ACTCT 7623935 3.4749377 67.955475 20-24
GAGTG 10351300 3.3785632 39.234093 90-99
TCCTG 8937145 3.3719099 64.440315 20-24
TATCT 5857070 3.3127866 48.368164 200-209
CTCCT 8367120 3.2835855 93.61903 20-24
GGATT 7761705 3.2699013 56.040672 40-44
AGACA 8476995 3.253981 37.069763 170-179
CTGCA 9195085 3.2470064 46.094173 25-29
GGTCC 10911580 3.189514 140.24062 9
CATCT 6954600 3.1698594 32.780876 170-179
AGCTG 9287330 3.152994 35.20578 60-69
GTCTC 8328155 3.142143 33.867794 30-34
CCATC 8427570 3.0954583 35.30334 45-49
GTCCC 10089890 3.0677388 74.02344 10-14
CCTCA 8283865 3.0426753 83.94593 5
GGAGT 9228755 3.012175 36.49412 90-99
GGCTG 10561400 2.967995 34.24897 90-99
AAGAA 6536790 2.914292 36.94948 180-189
AGTAG 7319830 2.8862154 28.778503 50-59
AGGGA 9346850 2.855306 39.89282 80-89
GTGAA 7104470 2.8012986 40.46144 10-14
TGTGC 7679600 2.7856123 56.62184 25-29
ATCTG 6342725 2.7793841 36.267284 200-209
GAGCT 8169660 2.773552 28.359015 60-69
CAGGG 10522735 2.76771 35.94335 80-89
GACAA 7201150 2.764235 24.61959 180-189
ATCTC 6007315 2.7380931 47.755684 170-179
GGAAG 8922345 2.7256267 29.900677 80-89
GTGTA 6422590 2.7057502 35.94371 230-239
ACCTT 5868600 2.6748679 71.86571 40-44
GAACA 6945955 2.6662757 34.632683 210-219
ATGAA 5596785 2.6659806 27.44682 210-219
CTATG 5968415 2.6153615 26.116482 50-59
GAGTC 7672460 2.6047554 27.434166 150-159
GGGAA 8512605 2.600458 33.667538 80-89
CACGG 9442765 2.5833678 44.404087 230-239
TGCAA 6268580 2.5709407 35.24315 200-209
CGCCA 9033480 2.5706162 39.41482 70-79
AGGGC 9671345 2.5437753 33.051785 150-159
CAGTG 7429570 2.5222957 77.03206 8
AGAAC 6545720 2.5126414 30.406998 190-199
CCCTC 7896805 2.497345 27.087734 20-24
AGGCT 7340350 2.492006 21.004719 25-29
CTACA 5838550 2.490709 20.23667 190-199
TCTGT 5252155 2.4590082 24.274822 210-219
ACGGC 8986440 2.4585257 48.68209 230-239
CCAAG 7418635 2.451894 38.194202 180-189
ATCAG 5972720 2.4495993 27.048208 45-49
GTGGA 7492920 2.445616 26.74786 100-109
CACTG 6925070 2.4454095 19.679094 30-34
CTACT 5263485 2.3990605 22.595015 50-59
CTTCA 5194575 2.3676517 50.67422 45-49
CTGTC 6272170 2.3664372 48.40408 30-34
TATAT 3586535 2.356045 22.891167 240-247
GGGCT 8377230 2.3541934 42.459293 90-99
TGAAC 5738070 2.353362 37.38657 210-219
ACAGC 7097090 2.3456218 26.39422 210-219
CCGCC 9562865 2.3430133 33.731388 70-79
CTCTC 5965030 2.3409114 96.66811 20-24
GCAGC 8514275 2.32935 33.791397 30-34
CAAAT 4622115 2.290099 35.016033 200-209
CAGGC 8276025 2.2641692 33.50386 70-79
ACAAC 5668505 2.2632723 20.990213 180-189
TATGC 5161240 2.261657 20.224167 50-59
GTATC 5155210 2.2590146 22.629915 200-209
GGCCG 9956020 2.2546659 24.861906 230-239
GCCTC 7401340 2.25031 74.11068 4
TGGTG 6385985 2.2269726 33.970646 35-39
TCCAA 5201355 2.2188835 40.989697 180-189
GCCTG 7568285 2.2122507 30.93033 210-219
AATGA 4639370 2.2099242 25.907747 210-219
TACAT 4166350 2.2055607 19.087719 190-199
AAATG 4630110 2.2055132 33.28852 200-209
GCTCC 7129815 2.1677554 33.43051 80-89
CCTTC 5504825 2.1603088 55.037525 45-49
CAGTA 5248020 2.1523771 21.019972 45-49
GTGGT 6155085 2.1464512 29.986399 120-129
GGGGG 10219510 2.1391253 94.00394 5
TATCA 3995720 2.1152334 17.12504 170-179
GTAGT 5009605 2.1104789 24.775715 120-129
GTGGG 7802845 2.1081405 29.84879 100-109
TCTAT 3691230 2.0877774 19.351517 110-119
CCTCT 5281810 2.0727892 48.18067 35-39
GCAGA 6503030 2.0663235 23.607517 140-149
TGCAG 6080810 2.0643995 42.93401 30-34
AACAG 5336145 2.0483336 33.234283 210-219
TGCAC 5764490 2.0355806 26.64203 25-29
CTCAC 5532925 2.032251 54.751183 20-24
ATGGA 5135075 2.0247645 12.810126 100-109
CAGAG 6371375 2.0244904 32.20749 170-179
GCTGT 5575070 2.022239 19.33434 230-239
GCTAT 4609340 2.0198145 32.176083 50-59
TGTGA 4789610 2.017798 18.354807 210-219
AGAGT 5044155 1.9889147 30.60549 150-159
TCTCT 4074020 1.9839958 33.50326 35-39
AACTA 3960405 1.9622444 35.471207 130-139
TGAGC 5767670 1.9580903 17.55756 200-209
TTCAG 4464560 1.9563717 47.461727 45-49
GGCTC 6664885 1.9481817 19.787663 40-44
AGCAC 5893400 1.9477963 20.030594 130-139
ATATC 3658965 1.936964 13.939421 170-179
TCCGC 6355760 1.9324107 41.672234 70-79
GGTCT 5326740 1.9321623 36.86632 15-19
CATAT 3645305 1.9297328 13.667953 160-169
CTGTA 4357500 1.9094577 23.73744 190-199
CCGTG 6510390 1.9030222 19.887484 230-239
TCCAT 4169870 1.900598 27.165047 45-49
GATGG 5816535 1.8984601 21.651573 100-109
GCACA 5727755 1.8930501 18.189245 130-139
TGGGA 5798370 1.8925313 17.307474 100-109
CAGAC 5723760 1.8917297 25.72928 140-149
TGGGG 6997215 1.8904786 122.09372 4
GCCAG 6894875 1.8863115 34.944157 70-79
GGATC 5546610 1.8830417 18.495626 70-79
ACCCT 5125100 1.8824564 43.255142 10-14
ACTAT 3552180 1.8804348 17.088997 130-139
CACAG 5689470 1.8803967 16.477688 140-149
GTCAC 5320025 1.8786292 38.34384 160-169
ACCAG 5664755 1.8722283 14.068537 190-199
CCTGC 6152960 1.8707513 50.517902 20-24
TGGTA 4437905 1.8696294 30.075771 120-129
AGGAC 5879770 1.8682839 21.16039 220-229
CCAGA 5650605 1.8675517 33.769444 170-179
AGAGA 5012895 1.849978 28.096498 170-179
CAGTT 4203675 1.8420517 24.365068 190-199
CTATT 3247790 1.8369654 17.829584 110-119
TAGTA 3594455 1.8293685 17.67377 120-129
GGTGG 6754465 1.8248935 18.073774 40-44
TGTCT 3895785 1.8239689 49.905643 30-34
GAGGA 5936490 1.8134981 27.385128 220-229
GCTGA 5330865 1.8097978 26.525217 200-209
CAAGG 5693695 1.809159 17.703777 80-89
TTCCA 3938640 1.7952051 21.360662 180-189
TCAGC 5063395 1.788007 12.267951 45-49
TTCTG 3813865 1.7856147 43.823753 30-34
GCCGA 6517240 1.7829976 18.867712 220-229
AGTCA 4325245 1.7739183 29.081131 160-169
ACCTG 4993190 1.7632161 30.70552 25-29
AGTTC 4010590 1.7574418 21.331486 190-199
TGACC 4953895 1.74934 14.089886 210-219
ATACT 3302450 1.7482338 25.94547 130-139
CCTGG 5969950 1.7450485 33.91336 2
GGATG 5326720 1.7385894 23.430965 100-109
GCAAA 4515050 1.7331482 31.831781 200-209
TACAA 3485045 1.7267197 16.88765 130-139
CTGAA 4196375 1.7210646 22.882284 200-209
TAGTG 4067360 1.7135237 25.723816 120-129
CATGA 4177175 1.7131902 22.552963 60-69
CTCAG 4785530 1.6898862 80.3904 6
ATGCA 4113250 1.6869725 16.364294 60-69
GTGCA 4901385 1.6639916 49.7496 25-29
ATTGG 3944420 1.6617309 19.93749 100-109
AACAC 4159005 1.6605718 21.57767 190-199
GGAGC 6246080 1.6428558 13.335726 200-209
AGGGG 6468130 1.635594 33.098545 90-99
TCTGA 3718995 1.6296648 22.120827 220-229
GGGGC 7471540 1.6267148 28.696262 90-99
CGATT 3689025 1.616532 35.669334 160-169
AGCTA 3886625 1.5940263 24.533136 50-59
TCTGC 4224760 1.5939667 32.96298 200-209
GCCGT 5445335 1.5917009 29.029385 230-239
GGGCC 7025570 1.5910288 29.487814 2
AGTGA 3956235 1.5599469 89.19705 9
TCTCA 3422295 1.5598587 25.266718 100-109
GGCTT 4296120 1.5583265 22.089327 25-29
CGTCC 5079890 1.5444943 18.665789 180-189
GCACT 4360135 1.539669 25.583094 25-29
GTTCT 3268915 1.5304744 22.365215 190-199
CTCCC 4835345 1.5291657 13.477078 190-199
AGTTA 2991815 1.5226598 16.01044 50-59
CCATG 4282625 1.5122985 16.066994 160-169
GAGCA 4750020 1.5093082 8.02728 200-209
CAGCT 4269360 1.5076143 11.955014 50-59
TTCTC 3084970 1.502341 20.983833 190-199
CGGCC 6376890 1.5021057 31.293846 230-239
GTCCG 5099360 1.4905703 34.127426 70-79
ACAAT 3008075 1.4903977 21.61156 180-189
CATCA 3487720 1.4878517 31.6277 45-49
TTAGT 2672125 1.4530305 24.651613 110-119
CTGGT 3974740 1.4417528 31.699354 35-39
GTTAC 3271405 1.4335307 14.851177 50-59
GTGAC 4218100 1.4320203 14.585432 210-219
ACTCC 3856415 1.4164666 16.330837 140-149
TACGC 3993430 1.4101765 24.10374 140-149
GGCAG 5331875 1.4023999 14.080239 150-159
CTCAA 3286890 1.402178 18.94221 150-159
CTTTA 2474725 1.3997161 39.970722 45-49
GGCCT 4781530 1.3976669 71.02212 3
GTCCA 3931225 1.3882104 28.577812 180-189
ATACA 2800350 1.387477 23.50491 35-39
GGGGT 5128590 1.3856213 116.68214 7
CGACA 4181915 1.3821427 17.016691 70-79
GAACT 3364740 1.3799851 15.067571 190-199
TTATT 1954495 1.3718065 22.02874 240-247
CAACC 3969775 1.3647048 15.485655 140-149
AGCAG 4293590 1.3642787 12.500157 50-59
AACCC 3968360 1.3642182 19.942335 140-149
CAACT 3181625 1.3572725 16.579645 130-139
CGTGT 3719165 1.3490486 23.654589 230-239
ATCTA 2542265 1.345811 20.175497 110-119
CCATA 3152430 1.344818 14.046455 160-169
ACAGA 3495490 1.3417796 21.026943 140-149
AAGCT 3258540 1.3364291 15.880211 200-209
ACATG 3257155 1.3358612 15.035107 190-199
ACAGT 3255920 1.3353548 12.191203 120-129
CTACG 3775560 1.3332413 16.202341 130-139
GAGAG 4358545 1.3314624 25.327972 220-229
TCACT 2917405 1.3297333 13.881653 190-199
GTGCG 4730585 1.3294027 17.193129 240-247
TGGAA 3354395 1.3226409 18.8716 120-129
ATTAT 2009845 1.3202952 9.8231535 50-59
CACGT 3735035 1.3189312 16.87106 180-189
TCAAG 3203705 1.3139396 22.979334 150-159
TAGCA 3190360 1.3084664 10.937694 130-139
CCTAC 3554770 1.3056718 17.740755 190-199
TGTCC 3450165 1.3017185 53.9664 15-19
AAGAG 3517950 1.2982777 18.494648 150-159
TGCGA 3820325 1.296978 14.231664 240-247
ACACA 3223925 1.2872212 7.887364 130-139
TGTGT 2857105 1.2860372 15.136987 240-247
GTGGC 4569205 1.2840512 14.221097 40-44
CCAGT 3602245 1.2720398 18.437601 190-199
AACCA 3169540 1.2655067 17.749382 190-199
TTACA 2385835 1.263001 9.311143 120-129
CTTCT 2593495 1.2629989 23.729998 30-34
CAATT 2372600 1.2559948 22.356686 180-189
TCCGT 3327800 1.2555511 13.038202 150-159
TGGAC 3697265 1.2551999 16.450058 80-89
AGACC 3793230 1.2536803 99.34437 9
CCTTT 2555925 1.2447028 45.810272 45-49
GATTG 2935860 1.2368381 23.42305 100-109
AGAGC 3892470 1.2368237 24.011137 220-229
CGGCT 4201370 1.2280831 21.396883 230-239
ACGTC 3476245 1.2275462 18.692879 180-189
ATATT 1868050 1.2271482 24.456875 240-247
TGAGT 2911825 1.2267125 21.75013 90-99
AGTCG 3608950 1.2252175 15.462585 150-159
AATTC 2311290 1.2235388 22.339033 180-189
ACATA 2468630 1.2231213 19.942373 130-139
ATCCG 3451115 1.218672 23.70474 70-79
AAGGC 3753405 1.1926361 29.864218 25-29
TACCA 2789235 1.1898799 10.486261 45-49
GTCGA 3494265 1.1862825 12.061551 150-159
GGGTG 4367295 1.1799377 15.1491 60-69
GAAGT 2987605 1.1780152 14.287355 140-149
ATCAT 2215160 1.1726499 15.296138 110-119
GATCC 3317555 1.1715089 23.553286 70-79
AGGCC 4270980 1.1684619 14.566713 2
GATCT 2647455 1.1601157 9.601801 220-229
TCGGA 3401500 1.1547894 92.75872 5
CTTCG 3046885 1.1495641 103.888596 3
GGACT 3374475 1.1456145 12.536137 90-99
AGGTC 3364185 1.1421212 18.820253 10-14
ATTAG 2238665 1.1393503 24.566267 110-119
ACAAG 2963165 1.1374412 18.095257 80-89
ACGCA 3431440 1.1341071 20.343178 140-149
GCAAG 3562150 1.1318654 17.54202 25-29
TCGAG 3329060 1.1301965 11.634923 150-159
TTGGG 3229040 1.126057 14.666988 100-109
CTTGA 2561535 1.1224654 22.938307 90-99
ACACC 3258225 1.1200925 13.504513 40-44
TTGAG 2657905 1.1197395 21.42626 90-99
AGCTC 3161265 1.116319 12.372261 210-219
GAACC 3376515 1.115954 10.436955 190-199
GCTTC 2948830 1.1125691 27.283236 2
GACCC 3904720 1.1111485 42.550537 10-14
CCGAT 3126670 1.1041026 23.92283 160-169
ATGGG 3371855 1.1005405 20.589825 100-109
ATCAA 2217605 1.0987469 12.285973 110-119
CGCTG 3747265 1.0953459 11.465379 190-199
TTCGG 3017810 1.0946467 99.957794 4
GCCCC 4453165 1.0910772 12.48241 80-89
ATTCC 2387060 1.0880057 20.794977 180-189
AAAAA 2005200 1.0799829 5.2719254 190-199
CAGCA 3257930 1.0767612 12.999121 45-49
AGATC 2604825 1.0683203 12.926899 210-219
GCACC 3743185 1.0651813 11.092984 130-139
GACAG 3346860 1.0634573 16.379454 70-79
CGCAG 3885315 1.0629511 15.89897 140-149
TAGAC 2591140 1.0627075 16.618467 170-179
CCCCT 3343125 1.0572549 9.695878 70-79
CACAT 2475365 1.0559839 13.017757 130-139
GGGAG 4165130 1.0532352 14.541436 5
GGAGA 3444665 1.0522873 83.24078 7
TACAG 2555800 1.0482135 8.719499 120-129
CTATA 1970410 1.0430855 13.491417 50-59
GTATA 2047860 1.0422416 9.490485 240-247
GAAGC 3277345 1.0413691 12.429372 200-209
TGAGG 3184800 1.0394876 12.362016 220-229
TTTCA 1836850 1.0389311 7.8489633 100-109
TGGCT 2852585 1.0347148 31.033184 40-44
ATGAG 2600935 1.025551 19.55477 60-69
ATGGT 2431150 1.0242106 9.480579 120-129
ATGAC 2494165 1.0229352 11.46338 160-169
ACCGC 3585825 1.020402 11.409103 220-229
GCCAA 3080770 1.018209 15.500797 180-189
GCTCT 2686445 1.0135734 16.722326 210-219
AAGGT 2565290 1.0114961 21.408186 15-19
CTCCG 3326620 1.0114285 9.578347 140-149
GAGCC 3688915 1.0092196 17.501202 220-229
AACTC 2360710 1.0070723 16.891043 190-199
AAACT 2026865 1.0042418 16.912678 130-139
CCGTC 3300930 1.0036178 10.53381 140-149
ACTCA 2349365 1.0022327 12.805756 190-199
CGAGG 3807320 1.0014087 14.480672 220-229
TTATA 1509905 0.9918777 12.677703 110-119
TAGTT 1822265 0.9908991 14.288382 50-59
CGTGA 2908700 0.9874868 19.655039 150-159
GATAC 2407570 0.9874198 25.443386 35-39
ACAGG 3099650 0.984907 15.915937 70-79
AGCCG 3597165 0.9841185 18.554598 220-229
ATGAT 1926510 0.9804815 8.546034 120-129
GTAGC 2883925 0.97907573 11.091099 50-59
AACTG 2376645 0.9747365 12.735805 60-69
TTTAT 1385440 0.97240245 11.757631 240-247
CGAGT 2847485 0.96670455 15.328271 160-169
GTAGA 2449850 0.96597797 16.437807 170-179
GCAGT 2833520 0.9619635 6.799044 50-59
TACAC 2252080 0.9607311 22.961447 35-39
GGGAC 3626455 0.95383704 9.596983 90-99
CGGAC 3485920 0.953684 10.597913 220-229
CAGAA 2483490 0.95331305 18.725512 140-149
AAGTT 1870675 0.95206475 18.496012 140-149
TAACA 1912760 0.9477067 9.182196 120-129
CCGAG 3448065 0.9433275 17.428837 220-229
ACCAA 2362520 0.9432868 16.213428 130-139
GAGAT 2363190 0.9318079 12.53896 210-219
TATTT 1317495 0.9247136 11.520337 240-247
AGCCC 3248135 0.924307 11.61707 80-89
GGTAT 2179895 0.9183603 8.923965 110-119
CATAC 2151820 0.91796046 13.293504 130-139
TTTAG 1682815 0.91507 28.303082 45-49
CCAAC 2658275 0.9138454 13.178209 130-139
TCATA 1719505 0.91026264 8.648397 110-119
GTTAT 1669395 0.9077725 10.156102 110-119
AATCA 1825340 0.90439314 13.235472 110-119
GCCAT 2559630 0.9038671 7.26182 60-69
TGGTT 2003395 0.9017661 9.149278 50-59
CCCAG 3168145 0.90154463 14.491835 80-89
GATGA 2282890 0.90014553 6.9580255 60-69
TATGG 2123885 0.89476407 11.908488 35-39
AGTCT 2041665 0.89465827 15.767699 8
AGAAG 2410140 0.8894473 16.377136 140-149
GTTTC 1895350 0.8873846 5.56375 100-109
CCCCA 2963605 0.8771981 16.640116 80-89
GACTG 2561425 0.86958873 12.128822 90-99
TCCTT 1779555 0.8666206 10.625659 1
CATGG 2549975 0.8657016 8.816765 200-209
CGGAG 3291320 0.86568934 71.826805 6
GCCTA 2448250 0.8645363 17.029827 190-199
ATAGT 1694580 0.8624427 15.738784 120-129
GTCTG 2370565 0.8598722 14.1501045 3
TTATG 1572450 0.85505635 12.592586 50-59
GTCTA 1941230 0.8506475 9.60178 30-34
GATCA 2059710 0.84475154 9.320068 110-119
TTACC 1843945 0.8404575 9.640459 45-49
TCTTC 1725610 0.84035003 45.752495 2
ATCCA 1969735 0.84028345 9.770486 180-189
GGTGC 2979930 0.8374285 12.0841055 60-69
CTATC 1830835 0.8344821 8.29483 110-119
GCTTG 2295250 0.83255345 17.386595 90-99
CGTAT 1893275 0.8296338 6.7948155 240-247
TCATC 1801695 0.82120025 10.516561 110-119
ATATA 1325140 0.81474274 11.201803 130-139
ACATC 1895625 0.8086684 5.6304035 110-119
ACGCT 2278225 0.80449635 14.10251 190-199
GGTTC 2213065 0.8027425 10.539408 160-169
ACAAA 1724730 0.79980636 12.81296 130-139
CATTA 1509795 0.79924744 12.683183 110-119
TGGCA 2352785 0.7987568 5.4867496 100-109
GGTAG 2434540 0.7946101 11.365268 120-129
CCTCC 2487575 0.7866894 9.975592 140-149
ATATG 1545285 0.78646016 5.644325 50-59
TGATG 1864475 0.7854781 11.937428 120-129
TATAC 1483765 0.78546786 9.313456 130-139
CTGAC 2213830 0.7817568 9.650788 220-229
TCAAC 1807805 0.7712046 7.6162043 110-119
TGACT 1735205 0.7603674 5.0815783 2
GGGAT 2329125 0.76020354 13.752641 100-109
TATGA 1492940 0.7598197 5.4831 50-59
CCAGC 2668960 0.7594939 6.7548413 80-89
TTGGA 1770785 0.7460078 6.577082 60-69
ACGCC 2614625 0.744032 13.510869 180-189
AACGC 2249255 0.7433893 15.089873 180-189
ATCTT 1313220 0.74276346 12.462182 3
GCGGA 2823485 0.7426384 8.885838 220-229
GACCA 2227020 0.7360406 6.33377 170-179
TATTG 1353190 0.73582864 6.9763193 240-247
GGATA 1862030 0.73420006 16.420979 35-39
GGTTA 1741600 0.7337125 8.0395355 50-59
TTTCT 1212125 0.7325044 9.224072 4
GGGCA 2777395 0.7305157 14.069294 150-159
GTTCA 1660820 0.7277718 12.822073 160-169
GACCG 2658395 0.7272882 8.896255 210-219
CAACG 2199880 0.72707075 15.40562 180-189
GATTA 1422270 0.7238527 5.7389226 50-59
CCCCC 2834135 0.72227466 13.209313 80-89
TTTAC 1276685 0.7220992 13.188756 45-49
ATAAA 1229905 0.70775104 7.4686227 110-119
CATCC 1914655 0.7032555 6.5310373 110-119
GAAAT 1469240 0.6998598 7.0779705 110-119
CGCCG 2969380 0.6994511 10.8727255 220-229
CACAA 1751115 0.69917023 8.438933 130-139
TCCGG 2379305 0.6954836 11.328892 70-79
TATAG 1362340 0.6933518 6.746935 120-129
CAGTC 1961295 0.6925807 5.7161927 210-219
AATAC 1384780 0.68611085 11.080049 130-139
CCCGT 2235780 0.6797685 10.300679 140-149
ATGCC 1923715 0.67931026 7.660816 50-59
GCGAC 2466400 0.67476195 7.4786797 70-79
TGCCA 1910265 0.67456067 6.9503436 60-69
GCCGC 2859555 0.6735813 12.020729 220-229
CAAAC 1679880 0.67072815 13.587333 130-139
AGATG 1671240 0.6589714 5.976191 120-129
AGGCA 2070890 0.6580207 6.231292 80-89
GACGA 2060565 0.6547399 9.947634 220-229
AAATA 1135760 0.6535751 12.306066 130-139
ACTTC 1430365 0.6519506 30.027857 4
TACTT 1142280 0.646079 11.131535 1
TCAAT 1190390 0.6301625 8.067676 110-119
CACCG 2214000 0.6300279 9.026278 45-49
AAGCA 1634745 0.6275136 5.774998 120-129
CCCTA 1700335 0.62453544 12.258668 110-119
TCCAC 1700130 0.62446004 10.334593 180-189
CACGC 2171975 0.6180691 11.424476 190-199
AATAA 1061095 0.61060905 6.840604 130-139
ACCTA 1420320 0.6059046 7.8447514 130-139
CACGA 1827560 0.6040172 8.859891 180-189
AATGG 1531755 0.6039724 6.660648 120-129
GGCCC 2559525 0.60290784 10.360365 70-79
GTTCC 1593635 0.6012652 7.290941 140-149
ACGAC 1812585 0.5990679 9.252918 220-229
CCGGC 2542535 0.5989058 9.148192 70-79
GGTGA 1828855 0.59692043 6.6635146 120-129
TGATT 1096810 0.596416 6.201399 50-59
ATTTC 1052945 0.59555066 6.805647 240-247
ACCCG 2090825 0.59497654 8.994851 140-149
CTTTG 1263425 0.5915234 7.334486 45-49
TAGCT 1349220 0.59122866 12.143495 50-59
GGGGA 2303385 0.5824563 9.283272 4
AGTAA 1219100 0.58070785 5.3006015 50-59
TTCAA 1086580 0.57520807 5.560778 200-209
ATTAA 933515 0.5739579 6.8759217 110-119
GGAGG 2206310 0.55790895 5.4221563 6
TCAGG 1642875 0.55774647 5.4576616 150-159
CCGTA 1560385 0.55100965 5.848906 230-239
GAAGA 1480590 0.5464026 6.470629 15-19
TAATA 880335 0.54126096 7.4402046 130-139
CGGCA 1977540 0.5410187 10.576593 70-79
CCACG 1898860 0.54034996 7.774333 180-189
ACATT 1020035 0.5399809 6.666634 110-119
CTGTT 1149530 0.5381988 5.7129035 240-247
CTGCG 1839045 0.5375627 11.740395 25-29
AAGAT 1126060 0.536389 5.9174557 15-19
CCTAT 1169560 0.5330774 5.059753 110-119
GCTAC 1504300 0.5312047 7.8425903 50-59
GTCCT 1405095 0.53013057 6.129603 45-49
TATGT 971725 0.52839816 6.8562117 140-149
CGGTT 1446195 0.52457666 10.425273 160-169
TTAGC 1192835 0.52270067 8.868084 45-49
AGTAT 1013660 0.515894 5.730143 110-119
GGGTT 1477545 0.5152615 6.4259534 100-109
AAATC 1036620 0.5136095 7.5601997 110-119
GTAAC 1222650 0.501447 7.1754336 120-129
GGTTT 1106935 0.49825245 5.9511814 100-109
TGACG 1457140 0.49469054 8.764508 220-229
CGCGG 2178485 0.49334535 6.4697137 220-229
ACCCC 1661755 0.49186322 8.469349 140-149
CATAA 992005 0.49150434 6.133809 110-119
TATCC 1077395 0.49106926 5.396016 110-119
TGGCC 1677240 0.49026632 15.26327 2
AACAT 976460 0.4838023 5.981453 110-119
CAATC 1080980 0.46114308 5.964514 110-119
CCGCG 1930100 0.45464393 6.6851573 220-229
ATGCT 1022700 0.44814748 6.848328 50-59
ATTCT 792270 0.44811168 7.207308 2
GCCTT 1183245 0.44642854 9.273109 1
TGCTA 996755 0.4367783 6.098833 50-59
TGCGC 1487125 0.43469468 8.632008 25-29
CCGGT 1485155 0.43411884 8.080276 160-169
GGTCA 1273460 0.43233225 5.539809 160-169
TAAAT 694625 0.42707992 7.321981 130-139
TTGGT 943880 0.42485833 5.1054583 120-129
CACTT 921445 0.41998836 7.075294 3
CTAAC 981395 0.41866037 5.8551984 110-119
GCATG 1221220 0.41459706 8.190661 60-69
CCTAA 965405 0.41183907 8.120792 110-119
TTTGA 741165 0.4030258 6.2382345 45-49
ATGGC 1186355 0.4027606 7.352509 1
ACGAG 1264035 0.40164435 7.6234884 180-189
CCGGG 1757350 0.39797404 5.3757143 80-89
AGCTT 905190 0.39665452 7.0349216 1
GCGCT 1352660 0.39538983 7.7994213 25-29
CATGC 1103090 0.38952774 6.859152 60-69
GAGGC 1463855 0.3850259 5.850914 35-39
GGTAA 975130 0.3844946 7.561066 120-129
TTCTT 605475 0.36589715 19.361643 1
CGGTG 1284625 0.36100903 5.435047 8
TGGGC 1247470 0.3505676 9.370865 1
TGCTG 954295 0.34615028 13.886143 1
ATGTG 815390 0.34351277 5.1857657 140-149
CGAGC 1214660 0.33230877 6.3923163 180-189
CTCTT 654970 0.31896204 8.670518 1
GCTTT 662045 0.3099631 5.138107 45-49
TCACA 725845 0.30964345 6.032691 5
CGGGA 1161850 0.305592 5.1277666 80-89
GCGAG 1160915 0.3053461 5.5089235 240-247
GACTT 692745 0.30356106 6.796055 1
CTCGG 1033905 0.302216 5.9106812 6
ATTGT 546790 0.29732984 5.736964 240-247
AACTT 556405 0.2945468 10.66288 3
CGCTT 777295 0.29326695 5.73017 1
GAGGT 896360 0.2925631 6.819219 7
TTCCT 591850 0.2882234 6.447384 4
CCCTT 714590 0.28043312 5.589687 3
GTCTT 573340 0.26843223 11.9609375 1
TAACT 503730 0.26666197 7.188944 2
TTGGC 731125 0.26520014 7.3997264 1
AATCT 500740 0.26507914 7.2039824 2
TGTAA 513260 0.26121947 7.9886775 25-29
CGGGG 1189705 0.25902435 9.340156 3
CCTCG 812400 0.2470028 6.484093 5
TGCTT 459035 0.21491574 11.190086 1
TCGGT 588570 0.21349132 7.060513 7
TCGCA 477145 0.16849142 5.6828218 7
TAGGC 464435 0.15767297 7.165769 1
TTGCT 267925 0.12543991 5.7700243 4
Produced by FastQC (version 0.10.1)