FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_7_SL248353.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_7_SL248353.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 5929637
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26122 0.44053286904409156 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20373 0.34357921066669006 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20314 0.3425842087804026 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19697 0.3321788500712607 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 19255 0.32472476814347995 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19000 0.320424336262068 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18671 0.314875935913109 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 17356 0.2926991989560238 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16806 0.28342375764317446 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16099 0.2715005994464754 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15760 0.2657835547100101 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15243 0.2570646398759317 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14971 0.25247751253575895 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14904 0.25134759513946636 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14439 0.24350563112042106 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14437 0.24347190224291976 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14364 0.2422407982141234 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14328 0.24163367841910055 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14274 0.24072299872656625 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13963 0.2354781582751187 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13878 0.2340446809813147 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13838 0.23337010343128928 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13638 0.22999721568116227 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 13524 0.22807466966358986 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13395 0.2258991570647579 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13242 0.22331889793591073 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11970 0.20186733184510286 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 11942 0.201395127560085 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11871 0.20019775240878995 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 11809 0.1991521572062506 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 11799 0.1989835128187442 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11777 0.19861249516623025 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 11518 0.19424460552981573 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 11477 0.19355316354103969 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11152 0.18807222094708326 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 10928 0.18429458666694098 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 10858 0.18311407595439652 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 10811 0.1823214473331167 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10686 0.18021339248928728 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 10335 0.17429397448781436 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 10237 0.1726412594902521 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 10141 0.17102227337019113 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 9914 0.16719404577379696 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 9736 0.16419217567618388 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 9679 0.16323090266739768 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 9630 0.16240454516861658 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 9481 0.15989174379477192 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9466 0.15963877721351238 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 9223 0.15554071859710805 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 9205 0.15523715869959662 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 9118 0.15376995252829134 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 9020 0.15211723753072912 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 8493 0.1432296783091444 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 8482 0.1430441694828874 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 8416 0.14193111652534549 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 8271 0.13948577290650338 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 8225 0.13871000872397418 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 7947 0.13402169475129758 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7753 0.13074999363367437 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7607 0.12828778557608164 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 7527 0.12693863047603082 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 7450 0.1256400686922319 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 7343 0.12383557374591396 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7150 0.12058073706704138 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 7148 0.1205470081895401 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 6618 0.11160885565170346 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6237 0.10518350448771147 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6206 0.10466070688644179 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 6138 0.10351392505139859 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 12031745 7.670508 66.12292 35-39
TATTA 5907855 7.28122 126.84925 240-247
TGGAT 9191725 7.276149 79.12634 35-39
TCACC 9308995 6.419221 74.02664 160-169
TCTCC 8243790 6.088902 85.79028 20-24
CTGTG 8657420 5.911756 85.81922 25-29
TCCCT 7853995 5.8009977 72.40836 15-19
AGTGG 9314915 5.709949 39.77163 100-109
CTGAG 8850640 5.642482 49.280483 15-19
TGTAT 5430905 5.5517244 60.494667 240-247
TTACT 5002105 5.3180265 111.585 240-247
ATTAC 5005515 4.9683704 104.075424 240-247
TGGGT 7456445 4.895733 44.741417 100-109
CCTGA 7319565 4.853139 50.7154 15-19
CTCTG 6657185 4.727817 73.091515 35-39
TTCAC 5448735 4.665351 74.45319 40-44
GACAC 7456745 4.615877 46.483006 230-239
ACACG 7403765 4.583082 48.98089 230-239
CTCCA 6627035 4.569817 40.455914 80-89
TGAGA 6006525 4.4391036 93.10805 15-19
ATTCA 4357850 4.3255115 110.55132 40-44
TCTGG 6308000 4.307444 134.7542 35-39
TACTG 5220385 4.297829 78.5264 240-247
AAGGG 7490680 4.2868876 39.481426 90-99
AGCCT 6294315 4.173361 50.566715 30-34
CCCTG 7286565 4.167578 84.53769 10-14
TGGAG 6765600 4.1472454 38.15468 90-99
GAAGG 7245825 4.1467576 31.419615 90-99
CAGCC 7735970 4.130886 54.54307 30-34
CCAGG 7994430 4.1046286 52.137535 80-89
ACCAT 5105995 4.0816574 66.81346 160-169
TCAGT 4869600 4.009035 100.12165 7
GAGAC 6733325 4.0076747 87.033035 8
GTATT 3887560 3.9740453 69.40985 240-247
CACCA 6166485 3.9699473 56.878506 160-169
TCCAG 5972385 3.95991 39.978916 80-89
ACTAC 4925420 3.9373083 34.554592 130-139
TACTA 3922615 3.8935065 28.41364 130-139
GGGTC 7322885 3.872211 124.08297 8
CACCT 5563475 3.8364158 43.969948 40-44
CTGGG 7175550 3.7943032 130.92961 3
ACTGT 4583465 3.7734666 67.838165 240-247
TGAAG 5044735 3.7282958 38.931046 150-159
GACTC 5588725 3.705529 51.067608 20-24
GATTC 4491160 3.6974735 58.03274 40-44
GGACA 6170245 3.67253 28.63942 220-229
AGACT 4748920 3.6501348 68.3738 15-19
CAAGA 4996585 3.585542 43.211147 180-189
ACTGG 5574130 3.5536327 45.00275 60-69
GCTGG 6680720 3.5326455 33.01195 2
CCTGT 4911615 3.488143 60.59454 25-29
ACTCT 4013100 3.4361224 67.283966 20-24
GAGTG 5553440 3.404203 39.29643 90-99
TCCTG 4727060 3.3570757 64.33917 20-24
TATCT 3109660 3.3060591 48.192223 200-209
CTCCT 4443815 3.2822225 93.38599 20-24
GGATT 4125395 3.2656534 55.57351 40-44
AGACA 4513205 3.2386692 37.067318 170-179
CTGCA 4862715 3.224158 45.9692 25-29
AGCTG 4989780 3.1810966 35.60124 60-69
GGTCC 5776915 3.1769826 140.38286 9
GTCTC 4433295 3.148449 33.698284 30-34
CATCT 3668435 3.1410112 32.345882 170-179
CCATC 4485955 3.0933883 35.406338 45-49
GTCCC 5365325 3.068718 73.71547 10-14
CCTCA 4438985 3.060999 84.54687 5
GGAGT 4898550 3.0027618 36.423756 90-99
GGCTG 5585980 2.9537668 34.244915 90-99
AAGAA 3497690 2.9096413 36.75273 180-189
AGTAG 3902565 2.8841784 28.704529 50-59
AGGGA 5023970 2.875199 39.778576 80-89
TGTGC 4091370 2.793809 56.58691 25-29
GAGCT 4378665 2.7914968 28.584352 60-69
GTGAA 3768245 2.78491 40.454163 10-14
GTGTA 3517985 2.784829 36.445145 230-239
ATCTG 3362660 2.7684047 36.08476 200-209
CAGGG 5601425 2.7653036 35.87011 80-89
GACAA 3822210 2.7428122 24.29745 180-189
GGAAG 4788910 2.740675 29.897793 80-89
ATCTC 3196845 2.7372234 47.37641 170-179
ACCTT 3140135 2.6886666 72.545685 40-44
GAACA 3702710 2.6570592 34.38538 210-219
ATGAA 2968535 2.6450436 27.205196 210-219
CTATG 3192765 2.6285338 26.570402 50-59
GGGAA 4571785 2.6164153 33.576344 80-89
GAGTC 4074975 2.5978878 27.639357 150-159
CACGG 5029680 2.5824192 44.234066 230-239
TGCAA 3321385 2.5528972 34.99744 200-209
AGGGC 5153465 2.5441551 32.99994 150-159
CGCCA 4754955 2.5390706 39.116932 70-79
CAGTG 3967500 2.52937 77.524025 8
CCCTC 4223490 2.512317 27.25128 20-24
AGAAC 3496900 2.5093703 30.422525 190-199
CTACA 3131080 2.5029392 20.385166 190-199
AGGCT 3892970 2.4818554 21.263065 25-29
ATCAG 3208470 2.4661078 27.131607 45-49
ACGGC 4788680 2.458681 48.648373 230-239
GTGGA 3995275 2.4490638 26.852331 100-109
CCAAG 3941730 2.4400115 37.98548 180-189
TCTGT 2766270 2.4393475 24.513037 210-219
CACTG 3662895 2.4286335 19.650429 30-34
CTACT 2808945 2.405093 22.68886 50-59
CTGTC 3345720 2.3760722 48.6031 30-34
CTTCA 2768945 2.370844 50.779892 45-49
GGGCT 4444535 2.3501909 42.49475 90-99
TATAT 1905020 2.347869 23.121897 240-247
CCGCC 5080940 2.3404312 33.57893 70-79
TGAAC 3041650 2.3378859 37.212734 210-219
GCAGC 4551530 2.3369195 33.542286 30-34
ACAGC 3764660 2.3304014 26.188229 210-219
CTCTC 3146635 2.3241193 96.23467 20-24
GTATC 2775650 2.2851322 22.545275 200-209
TATGC 2759745 2.2720375 20.66817 50-59
CAAAT 2446380 2.2670264 34.751526 200-209
GGCCG 5295620 2.2551973 25.181753 230-239
GCCTC 3937415 2.2520192 75.10874 4
CAGGC 4380980 2.2493532 33.415535 70-79
TGGTG 3424675 2.248564 34.32613 35-39
ACAAC 3004225 2.2421057 20.555632 180-189
TACAT 2231680 2.2151194 19.267395 190-199
GTGGT 3372020 2.2139919 30.513702 120-129
TCCAA 2761590 2.2075746 40.981712 180-189
GCCTG 4002400 2.2010977 30.879484 210-219
AATGA 2459005 2.1910388 25.617962 210-219
AAATG 2452430 2.1851802 33.0952 200-209
CCTTC 2940600 2.1719408 55.18116 45-49
GCTCC 3780740 2.1624084 33.27892 80-89
TATCA 2173400 2.1572716 17.312096 170-179
CAGTA 2803835 2.1550956 21.077168 45-49
GGGGG 5392020 2.1229272 93.568504 5
GTAGT 2669335 2.1130395 24.899233 120-129
GTGGG 4146740 2.1083446 29.735151 100-109
TCTAT 1953665 2.0770543 19.35183 110-119
CCTCT 2799940 2.0680485 47.89104 35-39
GCAGA 3469290 2.0649214 23.604185 140-149
TGCAG 3217325 2.051117 42.653595 30-34
GCTAT 2483120 2.0442984 32.781063 50-59
CTCAC 2957595 2.0394747 55.17843 20-24
GCTGT 2973145 2.030224 19.347881 230-239
AACAG 2827760 2.0291965 32.960007 210-219
TGCAC 3058020 2.0275793 26.687859 25-29
AGAGT 2728210 2.016275 30.809456 150-159
ATGGA 2725585 2.0143352 12.79298 100-109
CAGAG 3383690 2.0139725 31.99667 170-179
TGTGA 2532240 2.0045154 18.504204 210-219
AACTA 2125120 1.9693195 35.638878 130-139
TGAGC 3084795 1.966626 17.787207 200-209
AGCAC 3167710 1.9608772 20.278568 130-139
TCTCT 2130415 1.9538237 33.334423 35-39
TTCAG 2372960 1.953606 47.630295 45-49
GGTCT 2849670 1.945909 37.255535 15-19
GGCTC 3526005 1.9391071 19.8916 40-44
ATATC 1949565 1.9350979 13.993105 170-179
TCCGC 3374645 1.930141 41.510063 70-79
CATAT 1938630 1.924244 13.770095 160-169
CCGTG 3473685 1.9103338 20.186117 230-239
GCACA 3071880 1.9015566 18.379818 130-139
CTGTA 2309180 1.9010975 23.891014 190-199
TCCAT 2219180 1.9001206 27.123873 45-49
TGGTA 2399405 1.8993635 30.610716 120-129
GATGG 3087955 1.8928853 21.661898 100-109
TGGGA 3082005 1.8892385 17.390036 100-109
CAGAC 3045610 1.8852949 25.634445 140-149
TGGGG 3706735 1.8846309 121.85577 4
ACCCT 2731580 1.8836207 43.65948 10-14
GGATC 2952060 1.8820045 18.447948 70-79
CACAG 3037090 1.8800209 16.504839 140-149
GTCAC 2829075 1.8757805 38.380356 160-169
ACCAG 3030050 1.8756629 14.229836 190-199
CCTGC 3274165 1.8726709 50.910793 20-24
GCCAG 3640325 1.869074 34.704197 70-79
ACTAT 1882395 1.8684263 17.045977 130-139
AGGAC 3111525 1.8519795 21.158554 220-229
GGTGG 3642160 1.8517988 18.264671 40-44
CCAGA 2981160 1.845399 33.525932 170-179
CAGTT 2240425 1.8444929 24.792255 190-199
AGAGA 2668085 1.8409379 27.969809 170-179
CTATT 1723695 1.8325598 18.088184 110-119
TGTCT 2064620 1.8206197 50.16999 30-34
GCTGA 2846550 1.8147393 26.762833 200-209
GAGGA 3161785 1.8094776 27.336761 220-229
CAAGG 3038570 1.8085567 17.697817 80-89
TAGTA 1893545 1.8071691 17.486345 120-129
TCAGC 2718055 1.8021699 12.3429575 45-49
TTCCA 2097560 1.7959863 21.399622 180-189
TTCTG 2013270 1.7753382 44.153152 30-34
AGTTC 2152520 1.7721225 21.762434 190-199
AGTCA 2300255 1.7680316 29.08041 160-169
GCCGA 3439715 1.7660736 18.90295 220-229
ACCTG 2648525 1.7560691 30.807064 25-29
ATACT 1763015 1.7499322 26.124344 130-139
TGACC 2625285 1.7406601 14.09997 210-219
CCTGG 3163230 1.7396008 34.346024 2
GGATG 2830980 1.7353626 23.425453 100-109
TAGTG 2191270 1.7346044 26.05244 120-129
TACAA 1861390 1.7249244 16.939941 130-139
CATGA 2234095 1.7171795 22.773605 60-69
GCAAA 2391370 1.7160437 31.589838 200-209
CTGAA 2215720 1.7030562 23.023117 200-209
CTCAG 2558295 1.6962433 80.91839 6
ATGCA 2181010 1.6763772 16.168783 60-69
AACAC 2234090 1.6673404 21.592514 190-199
GTGCA 2609190 1.6634172 49.652843 25-29
ATTGG 2097930 1.6607165 20.108812 100-109
GGAGC 3347145 1.6524138 13.398393 200-209
AGGGG 3426870 1.6266714 32.975372 90-99
GGGGC 3959870 1.6214613 28.64564 90-99
GCCGT 2944950 1.6195589 29.4217 230-239
AGCTA 2104460 1.6175388 24.828152 50-59
TCTGA 1963390 1.6164161 22.10678 220-229
CGATT 1937855 1.5953937 35.210102 160-169
GGGCC 3735945 1.5909928 29.986217 2
TCTGC 2228710 1.5827911 32.809086 200-209
GGCTT 2295680 1.5676147 22.45781 25-29
TCTCA 1829160 1.5661752 25.558607 100-109
AGTGA 2100290 1.5522127 89.433205 9
CGTCC 2709400 1.5496515 18.74202 180-189
GTTCT 1754625 1.5472604 22.940779 190-199
CTCCC 2580285 1.5348668 13.687147 190-199
CAGCT 2313725 1.5340844 12.268743 50-59
GCACT 2310835 1.5321681 25.626438 25-29
AGTTA 1597640 1.524762 16.123613 50-59
CCATG 2287455 1.5166664 16.084476 160-169
TTCTC 1653505 1.5164449 21.47491 190-199
CGGCC 3418935 1.5142606 31.578514 230-239
GAGCA 2533925 1.5081921 8.088227 200-209
CATCA 1865100 1.4909334 31.675667 45-49
ACAAT 1608735 1.4907925 21.732533 180-189
GTCCG 2682355 1.4751464 33.84255 70-79
TTAGT 1425405 1.4571154 24.9476 110-119
GTTAC 1767330 1.4550042 15.160918 50-59
CTGGT 2121755 1.4488492 31.83512 35-39
GTGAC 2242085 1.4293796 14.684229 210-219
TACGC 2138830 1.4181226 24.26866 140-149
CTTTA 1325975 1.4097207 40.33951 45-49
ACTCC 2042450 1.408416 16.363495 140-149
CTCAA 1753645 1.4018381 19.012735 150-159
GGCCT 2542870 1.3984374 72.02377 3
GGCAG 2823225 1.3937658 14.1040945 150-159
CGTGT 2038400 1.3919297 24.052011 230-239
AAAAA 1386735 1.3908178 6.3506956 90-99
GTCCA 2088865 1.3849939 28.533068 180-189
ATACA 1483920 1.3751283 23.408817 35-39
AGCAG 2309995 1.3749089 12.793934 50-59
GGGGT 2704055 1.374834 116.348076 7
AACCC 2132735 1.3730425 20.095638 140-149
CGACA 2216455 1.372031 17.009909 70-79
CAACC 2128645 1.3704093 15.570943 140-149
CAACT 1705630 1.3634555 16.700699 130-139
GAACT 1766565 1.3578246 14.926177 190-199
CTACG 2027645 1.3444029 16.33326 130-139
CCATA 1681660 1.3442942 14.131081 160-169
AAGAG 1947950 1.3440559 18.651463 150-159
ATCTA 1351865 1.3418332 20.138985 110-119
GTGCG 2531805 1.3387734 17.389051 240-247
AAGCT 1741240 1.3383591 16.10891 200-209
ACAGA 1864265 1.3377938 20.864351 140-149
TTATT 1012125 1.3361061 21.249964 240-247
ACATG 1736320 1.3345777 15.151667 190-199
GAGAG 2329580 1.3332096 25.25181 220-229
TAGCA 1728620 1.3286592 11.152072 130-139
ACAGT 1725095 1.3259498 12.237198 120-129
CACGT 1993935 1.3220519 16.873188 180-189
CCTAC 1913490 1.3194889 17.883574 190-199
TGGAA 1776455 1.3128836 18.851145 120-129
ACACA 1757740 1.3118321 7.9330506 130-139
TGTCC 1843885 1.309495 54.492664 15-19
TCAAG 1702985 1.3089556 23.124222 150-159
TCACT 1526055 1.3066487 13.766691 190-199
TGTGT 1534730 1.3012748 15.143546 240-247
TGCGA 2041140 1.3012726 14.339882 240-247
ATTAT 1047350 1.2908214 9.584604 50-59
CCAGT 1932435 1.2812752 18.736038 190-199
GTGGC 2417945 1.2785662 14.259077 40-44
AACCA 1702490 1.270598 17.794874 190-199
CTTCT 1376060 1.2619976 23.882748 30-34
TTACA 1269260 1.2598411 9.405854 120-129
CCTTT 1370375 1.2567837 46.418907 45-49
AGACC 2026610 1.2545129 100.01936 9
AGAGC 2104530 1.2526162 23.9669 220-229
CAATT 1261515 1.2521536 22.502127 180-189
TCCGT 1762000 1.2513417 13.074346 150-159
TGGAC 1955245 1.2465127 16.537117 80-89
GATTG 1572315 1.244641 23.631767 100-109
ACGTC 1858040 1.2319485 18.69807 180-189
ATATT 995185 1.22653 24.736088 240-247
ACATA 1322925 1.2259364 20.080635 130-139
ATCCG 1848185 1.2254143 23.753265 70-79
TGAGT 1547465 1.2249697 21.871748 90-99
CGGCT 2219190 1.2204313 21.218052 230-239
AGTCG 1913905 1.2201573 15.544388 150-159
AATTC 1227960 1.2188476 22.473568 180-189
ATCAT 1223090 1.2140138 15.380976 110-119
TACCA 1494385 1.1945894 10.526365 45-49
GATCT 1439715 1.1852859 9.677077 220-229
GTCGA 1854880 1.1825277 12.140632 150-159
AAGGC 1984960 1.1814482 30.027412 25-29
TCGGA 1851470 1.1803538 93.31204 5
GGGTG 2312600 1.1758049 15.198697 60-69
GAAGT 1586600 1.1725717 14.332794 140-149
GATCC 1764100 1.1696627 23.608719 70-79
AGGCC 2256845 1.1587455 14.8273735 2
CTTCG 1629605 1.157317 104.73037 3
ATTAG 1196805 1.1422116 24.65149 110-119
ACGCA 1844130 1.1415542 20.476736 140-149
AGGTC 1786865 1.1391665 18.92848 10-14
TTGGG 1730145 1.1359739 14.886534 100-109
ACAAG 1578410 1.1326647 18.106077 80-89
ACACC 1753615 1.1289672 13.480324 40-44
GGACT 1770845 1.1289533 12.460939 90-99
TCGAG 1767205 1.1266329 11.714483 150-159
GCAAG 1889355 1.1245443 17.494816 25-29
CTTGA 1363640 1.122655 23.0511 90-99
GAACC 1813505 1.1225966 10.505565 190-199
TTGAG 1415640 1.1206174 21.56779 90-99
AGATC 1456740 1.1196856 13.073372 210-219
AGCTC 1686725 1.1183603 12.4769745 210-219
GCTTC 1573875 1.1177385 27.711052 2
GACCC 2089755 1.1158961 42.8746 10-14
TTCGG 1612030 1.1007812 100.749664 4
CGCTG 2000995 1.1004361 11.65104 190-199
ATCAA 1183015 1.0962837 12.358199 110-119
ATGGG 1787745 1.0958699 20.600254 100-109
GCCCC 2378805 1.095748 12.433337 80-89
ATTCC 1272885 1.0898777 20.905972 180-189
CAGCA 1748520 1.0823697 13.237597 45-49
CCGAT 1632255 1.0822448 23.44026 160-169
GCACC 2006230 1.0712951 11.164862 130-139
CGCAG 2083765 1.06988 16.01885 140-149
TAGAC 1385220 1.0647136 16.746984 170-179
CCCCT 1789245 1.0643213 9.7811985 70-79
CACAT 1330170 1.0633183 13.221962 130-139
GGAGA 1843705 1.0551454 83.400314 7
GGGAG 2222695 1.0550721 14.454772 5
GTATA 1105330 1.0549093 9.653648 240-247
GACAG 1772050 1.0547241 16.36807 70-79
GAAGC 1764085 1.0499833 12.598762 200-209
TACAG 1355195 1.0416358 8.850265 120-129
CTATA 1043295 1.035553 13.454767 50-59
TGAGG 1687545 1.0344483 12.34348 220-229
TGGCT 1509160 1.0305362 31.15272 40-44
ATGGT 1299760 1.028887 9.433789 120-129
ATGAG 1390545 1.027678 19.647755 60-69
TTTCA 963845 1.0247194 7.7711506 100-109
GCTCT 1431390 1.0165482 16.963541 210-219
ACCGC 1898645 1.0138465 11.379193 220-229
GAGCC 1972015 1.0125035 17.530287 220-229
AAGGT 1367065 1.0103252 21.336319 15-19
ATGAC 1314055 1.0100145 11.439184 160-169
CTCCG 1760265 1.0067903 9.628209 140-149
AAACT 1082740 1.0033602 16.998074 130-139
GTAGA 1355430 1.0017264 16.69026 170-179
ACTCA 1249985 0.9992196 12.67186 190-199
GCCAA 1613625 0.9988668 15.16814 180-189
CCGTC 1745810 0.9985226 10.645533 140-149
AACTC 1247645 0.997349 16.70499 190-199
CGAGG 2018485 0.99648285 14.480966 220-229
TAGTT 973165 0.99481463 14.419574 50-59
CGTGA 1547115 0.98632044 19.675108 150-159
GTAGC 1543095 0.98375773 11.062861 50-59
ACAGG 1648305 0.98107105 15.925219 70-79
TTATA 794900 0.9796858 12.577416 110-119
AGCCG 1907910 0.9795897 18.594189 220-229
GATAC 1268860 0.97527647 25.285828 35-39
CGAGT 1517590 0.9674976 15.333115 160-169
ATGAT 1011360 0.9652258 8.438192 120-129
TACAC 1202260 0.9610689 22.952354 35-39
ACCAA 1282085 0.9568424 16.290323 130-139
AACTG 1244545 0.95658743 12.5026865 60-69
AAGTT 999445 0.9538543 18.556221 140-149
CGGAC 1857290 0.95359963 10.608526 220-229
CAGAA 1327080 0.95231074 18.676767 140-149
GCAGT 1492630 0.95158505 6.834113 50-59
TAACA 1024675 0.94955224 9.174906 120-129
TTTAT 717170 0.94673616 11.233109 240-247
GGGAC 1911505 0.94366914 9.534636 90-99
CCGAG 1833450 0.9413593 17.48059 220-229
GAGAT 1260235 0.9313727 12.633895 210-219
CATAC 1162755 0.9294892 13.561938 130-139
TTTAG 907975 0.9281744 28.723713 45-49
CCAAC 1433390 0.9228082 13.293006 130-139
AGCCC 1725220 0.92124015 11.6197605 80-89
TCATA 927960 0.9210739 8.70128 110-119
GGTAT 1158175 0.9168087 8.918521 110-119
AATCA 986045 0.91375434 13.467075 110-119
TGGTT 1076295 0.9125746 9.362616 50-59
TATTT 686325 0.90601754 11.33871 240-247
GCCAT 1365035 0.9050681 7.3676453 60-69
GTTAT 883195 0.9028431 10.073996 110-119
CCCAG 1682450 0.8984017 14.493194 80-89
TATGG 1132320 0.8963419 12.171222 35-39
AGAAG 1294730 0.89334387 16.399618 140-149
CCCCA 1592400 0.88434684 16.55091 80-89
GATGA 1193040 0.88171244 6.7312555 60-69
AGTCT 1070890 0.8816403 15.537158 8
GTTTC 994790 0.87722397 5.4976635 100-109
TCCTT 955895 0.87666035 10.631161 1
GCCTA 1312105 0.86997354 17.223232 190-199
CGTAT 1052955 0.8668748 6.97347 240-247
ATAGT 908185 0.8667573 15.846466 120-129
CATGG 1358595 0.86613476 8.854952 200-209
CGGAG 1754360 0.86609 72.16694 6
GACTG 1357275 0.8652932 12.053943 90-99
ATCCA 1065075 0.85140526 9.87055 180-189
GTCTG 1246220 0.8509866 14.404308 3
GATCA 1102170 0.8471545 9.3397665 110-119
TTACC 988780 0.84661955 9.613483 45-49
GTCTA 1027500 0.84591836 9.849474 30-34
TTATG 826445 0.8448306 12.49725 50-59
TCTTC 916625 0.84064543 46.065453 2
CTATC 980435 0.8394744 8.352685 110-119
GGTGC 1584095 0.83764124 12.161108 60-69
GCTTG 1225375 0.8367523 17.537857 90-99
TCATC 959425 0.8214851 10.520553 110-119
CATTA 823110 0.81700194 12.466454 110-119
ACGCT 1223320 0.81110597 14.303316 190-199
ACATC 1013465 0.810149 5.728824 110-119
GGTAG 1315810 0.80657846 11.54765 120-129
GGTTC 1178550 0.80477774 10.772946 160-169
ACAAA 927670 0.80258995 12.753795 130-139
ATATA 696300 0.80119497 10.982332 130-139
TGGCA 1250570 0.7972665 5.4092584 100-109
CCTCC 1339620 0.7968646 9.967114 140-149
TATAC 788910 0.7830557 9.210991 130-139
ATATG 820480 0.783053 5.6599817 50-59
TGATG 981940 0.7773015 11.788107 120-129
CTGAC 1163220 0.7712575 9.585812 220-229
TCAAC 958635 0.7663187 7.605198 110-119
GGGAT 1240190 0.760224 13.78026 100-109
CCAGC 1420925 0.7587515 6.704325 80-89
TATGA 784850 0.74904823 5.391435 50-59
ATCTT 703000 0.7473999 12.554761 3
GGTTA 941470 0.74526554 8.23878 50-59
GCGGA 1502970 0.7419841 8.943113 220-229
GTTCA 892980 0.7351709 13.088565 160-169
TTGGA 926340 0.73328865 6.4421997 60-69
CCCCC 1530200 0.7330643 13.219487 80-89
AACGC 1177850 0.72911316 14.790305 180-189
GACCA 1175900 0.7279061 6.30772 170-179
ACGCC 1362610 0.72761226 13.257218 180-189
GGATA 983460 0.7268231 16.157537 35-39
CATCC 1053815 0.7266812 6.688697 110-119
GGGCA 1471905 0.726648 14.090013 150-159
GACCG 1413270 0.7256237 8.931977 210-219
TATTG 706040 0.721747 6.75857 240-247
TTTCT 631810 0.7194767 8.863212 4
GATTA 751965 0.71766335 5.6899242 50-59
CGCCG 1618910 0.71702194 10.9951315 220-229
CAACG 1156280 0.715761 15.105538 180-189
TTTAC 671550 0.7139636 13.099625 45-49
ATAAA 654280 0.7028666 7.2658553 110-119
CACAA 938435 0.70037043 8.345201 130-139
GAAAT 784420 0.698939 7.213562 110-119
TATAG 722805 0.6898335 6.70237 120-129
TCCGG 1249410 0.68710613 11.285717 70-79
ATGCC 1035880 0.68682635 7.8350487 50-59
TGCCA 1034420 0.6858583 7.076075 60-69
CCCGT 1187825 0.67938095 10.427646 140-149
CAGTC 1024595 0.679344 5.6431475 210-219
CAAAC 908640 0.6781339 13.685172 130-139
GCCGC 1524895 0.6753823 12.181745 220-229
AATAC 728775 0.6753457 10.882507 130-139
GCGAC 1311715 0.67348176 7.4754343 70-79
GACGA 1100685 0.65512764 9.875354 220-229
AAATA 608705 0.6539072 12.032758 130-139
ACTTC 756170 0.6474528 30.117506 4
AGGCA 1087615 0.6473484 6.107968 80-89
TACTT 608440 0.64686775 11.142174 1
TCCAC 934855 0.64464986 10.448802 180-189
AGATG 867955 0.6414594 5.8604236 120-129
TCAAT 636835 0.6321092 8.214668 110-119
CCCTA 908425 0.62642425 12.358011 110-119
AAGCA 872770 0.6262985 5.8086567 120-129
CACCG 1165510 0.6223639 9.081076 45-49
CACGC 1164220 0.6216751 11.566747 190-199
ACCTA 763395 0.6102467 7.824689 130-139
CACGA 983140 0.6085838 8.909667 180-189
GGCCC 1369615 0.6066082 10.41221 70-79
AATAA 564520 0.606441 6.70727 130-139
ATTAA 521865 0.600482 6.6309924 110-119
GTTCC 844700 0.59989125 7.370594 140-149
CCGGC 1353770 0.59959036 9.119661 70-79
GGTGA 973845 0.5969573 6.660097 9
CTTTG 676450 0.596506 7.2172937 45-49
ACCCG 1116495 0.5961907 9.139046 140-149
AATGG 806425 0.5959859 6.62337 120-129
ACGAC 959360 0.5938635 9.208065 220-229
TAGCT 717835 0.59097785 12.116145 50-59
TGATT 569395 0.5820621 6.15487 50-59
ATTTC 545375 0.5798196 6.637803 240-247
GGGGA 1218575 0.5784349 9.138781 4
CCGTA 869840 0.57673573 6.039393 230-239
AGTAA 640875 0.5710366 5.114195 50-59
GAAGA 820650 0.56623596 6.269236 15-19
TTCAA 567380 0.5631696 5.487588 200-209
GGAGG 1179010 0.5596541 5.4761286 6
TCAGG 873655 0.55697465 5.4460897 150-159
CCACG 1021715 0.5455797 7.8280864 180-189
CTGTT 611870 0.5395582 5.8575034 240-247
AAGAT 603595 0.53781915 5.685081 15-19
CGGCA 1046650 0.53738785 10.520336 70-79
TTAGC 651550 0.5364068 9.140429 45-49
GTCCT 755070 0.53623754 6.3036194 45-49
TAATA 464825 0.5348491 7.3399324 130-139
CTGCG 971570 0.53430957 11.913012 25-29
ACATT 538265 0.5342707 6.6030884 110-119
CCTAT 622480 0.53298384 5.203497 110-119
CGGTT 779770 0.53246915 10.662735 160-169
GCTAC 799955 0.5303994 7.832509 50-59
TATGT 513565 0.5249901 6.7600017 140-149
AAATC 560905 0.5197829 7.673336 110-119
AGTAT 533390 0.50905883 5.631373 110-119
GGGTT 772475 0.5071895 6.2766976 100-109
ACCCC 900390 0.5000359 8.476469 140-149
GTAAC 649850 0.49949047 7.2244787 120-129
AGTTT 488310 0.49917322 5.0235476 140-149
CGCGG 1171660 0.4989641 6.6228375 220-229
TGACG 781720 0.498364 8.763901 220-229
GGTTT 579515 0.49136218 5.8725934 100-109
CATAA 526830 0.48820612 5.98599 110-119
TATCC 570095 0.4881304 5.352899 110-119
TGGCC 877325 0.48248005 15.193577 2
AACAT 514065 0.47637692 5.876246 110-119
CAATC 578820 0.4627002 6.0878506 110-119
CCGCG 1035605 0.45867375 6.851527 220-229
ATGCT 543715 0.44762868 6.9597287 50-59
GCCTT 618765 0.43943608 9.324679 1
TGCTA 532935 0.43875375 6.209035 50-59
ATTCT 411725 0.4377286 7.2869506 2
CCGGT 795515 0.43748906 8.240194 160-169
TGCGC 791070 0.4350446 8.767353 25-29
GGTCA 676495 0.43128076 5.460975 160-169
TTGGT 507525 0.43032292 5.2573776 120-129
TAAAT 369485 0.42514646 7.223131 130-139
CTAAC 526620 0.42097226 5.8844976 110-119
CACTT 482525 0.41315067 7.0185575 3
CCTAA 515120 0.4117793 8.115159 110-119
GCATG 644180 0.4106792 8.144425 60-69
ACGAG 677365 0.4031677 7.680074 180-189
TTTGA 394305 0.40307692 6.2295823 45-49
GCGCT 726180 0.3993587 7.94622 25-29
ATGGC 624740 0.39828578 7.12557 1
TACCT 462545 0.39604327 5.151577 2
CCGGG 920185 0.39187074 5.2957344 80-89
AGCTT 473645 0.38994157 7.033526 1
GGTAA 524375 0.38753772 7.60601 120-129
CATGC 583175 0.3866664 6.7695165 60-69
GAGGC 776720 0.38345 6.0061707 35-39
CGGTG 715555 0.37837273 5.772562 8
TTCTT 321185 0.36575097 19.321037 1
TGGGC 656575 0.34718516 9.607967 1
ATGTG 431460 0.34154275 5.1033697 140-149
TGCTG 497755 0.33989412 13.75963 1
CGAGC 649835 0.3336487 6.4480305 180-189
CTCTT 348390 0.31951174 8.954465 1
CTCGG 580240 0.31909978 6.297531 6
TCACA 397095 0.3174319 6.382846 5
GCTTT 348620 0.30741948 5.026551 45-49
GCGAG 621880 0.30700886 5.5474644 240-247
CGGGA 608980 0.3006404 5.0870647 80-89
GACTT 363550 0.29930276 6.9511495 1
CGCTT 415040 0.29475415 5.8813295 1
AACTT 295150 0.29295978 10.731859 3
ATTGT 286270 0.2926385 5.59257 240-247
GAGGT 475880 0.29170963 6.906414 7
TTCCT 313780 0.2877706 6.2522006 4
CCCTT 379070 0.27998284 5.661176 3
GTCTT 306520 0.27029496 11.991102 1
AATCT 266210 0.26423454 7.316905 2
TAACT 264115 0.2621551 7.0238857 2
TTGGC 380555 0.25986356 7.540296 1
TGTAA 269415 0.25712538 7.772582 25-29
CGGGG 615950 0.25221512 9.223256 3
CCTCG 433060 0.24769029 6.8990617 5
TCGGT 348750 0.23814538 7.473405 7
CTGGC 403890 0.22211708 5.0846786 3
TGCTT 245575 0.21655254 11.284142 1
TCGCA 260050 0.17242268 6.0476418 7
TAGGC 241930 0.15423581 7.0554795 1
TTGCT 140080 0.123525105 5.592247 4
Produced by FastQC (version 0.10.1)