FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_3_SL248349.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_3_SL248349.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9542262
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 47536 0.49816280458448947 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 38592 0.4044324081648565 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 35974 0.3769965653846017 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33640 0.35253695612214375 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 32257 0.3380435372661115 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30052 0.3149358087212445 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 29691 0.3111526386510871 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29437 0.30849079599784623 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 29377 0.30786201426873416 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29100 0.30495913861933366 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27987 0.29329523754430553 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27860 0.2919643162176851 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27084 0.2838320725211695 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27073 0.2837167958708323 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26822 0.281086392304047 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25747 0.2698207196574565 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25643 0.2687308313269956 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25375 0.2659222729369619 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25009 0.26208670438937853 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23769 0.24909188198772997 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23661 0.2479600748753283 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23569 0.24699594289068985 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23181 0.24292982104243208 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23023 0.24127402915577043 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22966 0.24067668651311397 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22676 0.23763757482240583 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22260 0.23327802150056245 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20769 0.21765279553212855 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20694 0.2168668183707385 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20608 0.21596556455901125 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20453 0.21434121175880522 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20135 0.21100866859451148 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20005 0.20964630818143537 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19888 0.20842018380966693 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19553 0.20490948582212476 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19435 0.20367288175487114 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18752 0.19651524973847923 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 18598 0.19490137663375834 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 18591 0.1948280187653619 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18141 0.1901121557970217 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18002 0.18865547812457883 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 17863 0.187198800452136 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17808 0.18662241720044995 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 17659 0.18506094257315509 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 17067 0.1788569628459164 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16873 0.1768239019217875 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 16583 0.17378479023107937 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16528 0.17320840697939335 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16136 0.1691003663491948 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 16122 0.16895365061240197 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16064 0.16834582827426034 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15633 0.16382907952013892 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 15427 0.16167026225018766 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 15378 0.1611567571714128 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15124 0.1584949145181719 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 15097 0.1582119627400715 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15032 0.15753078253353345 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13634 0.1428801682452232 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 13495 0.14142349057278034 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13426 0.1407003915843015 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13346 0.13986201594548547 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 12934 0.13554438140558286 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12041 0.12618601333729884 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11904 0.12475029505582638 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11639 0.12197317575224827 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11400 0.11946852853128534 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 10779 0.11296063763497587 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10760 0.11276152342075704 No Hit
TAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 9974 0.10452448276938948 No Hit
AAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 9869 0.10342411474344342 No Hit
ATGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 9599 0.1005945969624393 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 19607465 7.7641935 66.9447 35-39
TATTA 9802335 7.566469 131.01207 240-247
TGGAT 14862355 7.325563 80.342255 35-39
TCACC 15165660 6.4831605 75.73346 160-169
TCTCC 13433960 6.133758 86.445015 20-24
CTGTG 14205105 6.0078144 87.453964 25-29
TCCCT 12852760 5.8683906 73.40716 15-19
AGTGG 15336545 5.844901 40.328884 100-109
CTGAG 14426975 5.712815 50.569622 15-19
TGTAT 8898395 5.672426 60.523438 240-247
TTACT 8295335 5.4943504 115.93414 240-247
ATTAC 8323605 5.1617465 108.96668 240-247
TGGGT 12183500 4.959283 46.351864 100-109
CCTGA 11954880 4.9186435 52.021725 15-19
CTCTG 10839100 4.7631097 74.19643 35-39
TTCAC 8864540 4.716937 76.48593 40-44
ACACG 12236190 4.713561 50.05752 230-239
GACAC 12144305 4.6781654 46.98577 230-239
CTCCA 10871845 4.6475987 41.529133 80-89
TGAGA 9826660 4.5348415 95.9717 15-19
TACTG 8643840 4.426754 81.54244 240-247
AAGGG 12285270 4.383662 40.055286 90-99
ATTCA 7063610 4.3803816 114.70485 40-44
TCTGG 10199335 4.31364 136.6682 35-39
GAAGG 11854775 4.230052 31.811947 90-99
TGGAG 11076475 4.2213483 39.857132 90-99
AGCCT 10227020 4.207743 52.1192 30-34
CCCTG 11876435 4.192807 85.999016 10-14
CAGCC 12607200 4.167161 56.21536 30-34
ACCAT 8315620 4.1428704 69.423454 160-169
CCAGG 12990560 4.132608 53.842392 80-89
TCAGT 8028350 4.111544 98.8497 7
GAGAC 11052980 4.097859 90.938446 8
ACTAC 8154445 4.062573 37.086967 130-139
GTATT 6336180 4.0391006 70.60298 240-247
CACCA 10048785 4.0219975 58.73544 160-169
TCCAG 9739330 4.0070915 40.976334 80-89
TACTA 6434000 3.9899392 29.740637 130-139
GGGTC 12069620 3.9469523 127.546 8
ACTGT 7588370 3.8862178 70.45287 240-247
CACCT 8937535 3.8207023 44.74869 40-44
TGAAG 8241740 3.803427 40.588875 150-159
CTGGG 11592060 3.7907832 134.57463 3
GACTC 9156990 3.7674963 52.198757 20-24
AGACT 7815155 3.7473047 70.27272 15-19
CAAGA 8302605 3.7273371 45.745792 180-189
GATTC 7276600 3.7265515 59.757217 40-44
GGACA 9894315 3.6682875 29.151869 220-229
GCTGG 10986700 3.5928211 33.66949 2
ACTGG 9068620 3.5910058 45.29939 60-69
CCTGT 7966035 3.500576 61.303574 25-29
ACTCT 6493375 3.4552097 68.77141 20-24
GAGTG 8951185 3.4113803 39.927975 90-99
TATCT 5117655 3.389639 49.795837 200-209
AGACA 7476325 3.35639 39.665745 170-179
GGATT 6788280 3.3459013 57.256035 40-44
AGCTG 8426540 3.3367538 38.344078 60-69
TCCTG 7575690 3.329044 63.91092 20-24
CTCCT 7139135 3.2596292 93.64071 20-24
CTGCA 7878290 3.2413962 46.51763 25-29
GGTCC 9448515 3.2103848 141.6944 9
CATCT 5941240 3.1614115 32.768715 170-179
GTCTC 7173555 3.1523306 34.39573 30-34
CCATC 7297515 3.1196105 36.41192 45-49
CCTCA 7164910 3.0629234 83.26073 5
GTCCC 8658360 3.0567117 74.823586 10-14
AAGAA 5787995 3.0282636 39.551907 180-189
GGAGT 7925505 3.0204842 38.23117 90-99
AGTAG 6485365 2.992889 29.98608 50-59
GGCTG 8994210 2.9412458 34.74776 90-99
AGGGA 8238570 2.9397082 41.729637 80-89
GAGCT 7382915 2.9234977 31.027796 60-69
TGTGC 6747835 2.8538852 58.200375 25-29
ATCTG 5471520 2.8021195 37.402496 200-209
GGAAG 7840055 2.797509 31.44475 80-89
GTGAA 6055610 2.7945642 39.570217 150-159
GACAA 6191845 2.7797408 25.157858 180-189
CAGGG 9072265 2.7777097 37.272297 80-89
GTGTA 5589235 2.7548995 37.750027 230-239
ACCTT 5143755 2.7370594 73.52996 40-44
GAACA 6096040 2.7367306 35.721764 210-219
ATCTC 5123055 2.7260442 48.43624 170-179
CTATG 5315355 2.722143 28.750357 50-59
ATGAA 4770695 2.6659033 28.067043 210-219
GGGAA 7409805 2.6439862 34.99572 80-89
GAGTC 6618715 2.6208885 29.33815 150-159
AGAAC 5815100 2.6106067 31.280996 190-199
CACGG 8202895 2.6095374 44.84748 230-239
TGCAA 5377045 2.5782504 36.51722 200-209
ATCAG 5374435 2.5769992 28.650751 45-49
CCCTC 6970070 2.5567083 27.21308 20-24
AGGGC 8301240 2.5416403 33.311573 150-159
CAGTG 6392060 2.5311375 76.43558 8
CGCCA 7651930 2.5292552 39.834785 70-79
TATAT 3253360 2.511284 25.452026 240-247
CCAAG 6517550 2.5106564 40.047848 180-189
CTACA 5034375 2.5081432 20.131388 190-199
ACGGC 7831295 2.4913225 49.779716 230-239
AGGCT 6273625 2.4842393 20.981306 25-29
GTGGA 6436525 2.4530199 27.469019 100-109
TCTGT 4440945 2.4291327 25.792465 210-219
CACTG 5873485 2.4165514 20.293884 30-34
GCAGC 7579170 2.4111156 34.71586 30-34
CTGTC 5477420 2.406985 50.204388 30-34
CTACT 4496005 2.392383 23.736628 50-59
TGAAC 4919555 2.3588874 38.50181 210-219
CTCTC 5112810 2.3344376 97.75538 20-24
ACAGC 6056790 2.333165 27.439938 210-219
CCGCC 8224760 2.332725 33.942368 70-79
TATGC 4553155 2.3317988 21.984306 50-59
CAAAT 4014515 2.3308866 36.167816 200-209
GGGCT 7108460 2.3245766 42.867332 90-99
TCCAA 4652770 2.318026 44.331062 180-189
CTTCA 4334715 2.3065584 50.98257 45-49
GGCCG 8718365 2.2904713 25.956345 230-239
GTATC 4466500 2.2874203 23.182863 200-209
GTGGT 5613135 2.2848215 32.303986 120-129
TGGTG 5582785 2.2724676 35.606716 35-39
CAGGC 7083230 2.2533453 33.683437 70-79
CAGTA 4695340 2.2513785 22.375355 45-49
ACAAC 4820650 2.2486153 20.383595 180-189
AAATG 4006275 2.2387395 34.407036 200-209
GCCTC 6326990 2.2336543 72.904816 4
AATGA 3988955 2.2290606 26.543545 210-219
GCTCC 6277810 2.2162921 34.033134 80-89
TACAT 3549205 2.2009811 19.049623 190-199
GTAGT 4445210 2.1910164 26.356342 120-129
GCCTG 6421785 2.1819727 31.10013 210-219
GCTAT 4234510 2.1686115 35.111607 50-59
TATCA 3490185 2.1643808 17.765059 170-179
GGGGG 8873230 2.15934 96.72309 5
CCTTC 4672170 2.1332474 55.25773 45-49
GTGGG 6770090 2.1307743 30.295977 100-109
TCTAT 3171780 2.1008036 21.074253 110-119
GCAGA 5646935 2.0935845 24.762924 140-149
GCTGT 4939225 2.0889637 19.568111 230-239
AACTA 3586675 2.0824761 38.654205 130-139
TGCAG 5251990 2.0796907 44.046623 30-34
CCTCT 4554365 2.0794592 48.98186 35-39
AACAG 4557575 2.0460587 34.39361 210-219
TGCAC 4942200 2.0333889 27.786839 25-29
AGCAC 5270520 2.030282 21.529104 130-139
CAGAG 5455260 2.0225215 33.071384 170-179
TGGTA 4102195 2.0219467 32.24834 120-129
TGAGC 5099225 2.0191987 18.610416 200-209
CTCAC 4721355 2.0183296 55.68499 20-24
ATATC 3253940 2.0178773 14.669345 160-169
TGTGA 4086795 2.014356 20.034607 210-219
ATGGA 4341105 2.0033486 12.635068 100-109
AGAGT 4305355 1.9868501 31.9124 150-159
GGCTC 5835825 1.9828771 20.961329 40-44
CATAT 3186975 1.9763502 15.452207 160-169
CCGTG 5734110 1.9483168 20.82126 230-239
GGTCT 4595340 1.9435233 36.175056 15-19
TCCGC 5490940 1.9384987 42.05101 70-79
TTCAG 3782940 1.93735 47.812557 45-49
TCTCT 3403360 1.9342338 33.60099 35-39
CTGTA 3773175 1.9323492 24.971384 190-199
CAGAC 4981940 1.919117 26.76724 140-149
GCACA 4979075 1.9180136 19.202932 130-139
CTATT 2877515 1.9058996 21.116213 110-119
GGATC 4805145 1.9027485 18.433815 70-79
TCCAT 3564510 1.8967223 27.768232 45-49
ACTAT 3055765 1.8949825 17.604382 130-139
ACCAG 4899050 1.8871865 14.372488 190-199
TGGGG 5989285 1.8850288 125.72682 4
GTCAC 4573950 1.8818783 38.8669 160-169
CCAGA 4873715 1.8774271 34.580936 170-179
GGTGG 5961295 1.8762196 18.856575 40-44
GCCAG 5881960 1.8711922 35.602577 70-79
AGAGA 4328560 1.8702618 28.921345 170-179
AGGAC 5034855 1.8666574 21.217033 220-229
CAGTT 3643055 1.8657111 25.900528 190-199
GATGG 4874495 1.8577157 21.231794 100-109
TGGGA 4864885 1.8540533 17.075106 100-109
GCTGA 4676740 1.8519026 28.140295 200-209
ACCCT 4319665 1.8466114 44.081318 10-14
CCTGC 5227070 1.845343 50.180313 20-24
TGTCT 3342760 1.8284413 51.62772 30-34
GAGGA 5099985 1.8197901 27.711576 220-229
CACAG 4722995 1.8193675 16.218975 140-149
ATACT 2933335 1.8190594 27.593178 130-139
TAGTG 3688570 1.8180735 27.166197 120-129
TTCCA 3415500 1.8174322 23.189714 180-189
TCAGC 4413745 1.8159647 11.573071 45-49
TAGTA 3023860 1.8047692 17.665262 120-129
GCCGA 5664005 1.8018556 19.863403 220-229
AGTCA 3752270 1.7991837 30.078281 160-169
TACAA 3080600 1.7886417 18.912285 130-139
CAAGG 4810065 1.7833173 17.56686 80-89
AGTTC 3466835 1.7754637 23.134718 190-199
GCAAA 3924975 1.7620621 33.129253 200-209
AGCTA 3657470 1.7537278 26.865662 50-59
CATGA 3654150 1.7521361 24.112158 60-69
ACCTG 4218465 1.7356199 30.927277 25-29
TGACC 4208435 1.731493 14.962167 210-219
CTGAA 3596120 1.7243111 24.330957 200-209
TTCTG 3151425 1.723784 43.52492 30-34
GGAGC 5600125 1.7146237 13.379191 200-209
CTCAG 4133775 1.7007755 79.73466 6
GTGCA 4292805 1.6998713 51.19435 25-29
GGATG 4419500 1.6843128 22.680473 100-109
ATTGG 3414825 1.6831461 21.753302 100-109
CCTGG 4950235 1.6819743 34.470085 2
ATGCA 3506420 1.6813008 16.436367 60-69
AACAC 3603455 1.6808487 23.08962 190-199
CAGCT 3971820 1.6341417 13.475444 50-59
GCCGT 4770450 1.6208876 30.324892 230-239
AGGGG 5499995 1.6207205 33.449856 90-99
TCTGC 3660765 1.6086782 33.784496 200-209
TCTCA 2994685 1.593511 27.079342 100-109
CGATT 3109105 1.5922602 35.341145 160-169
CTCCC 4340720 1.5922302 14.430878 190-199
GGGGC 6280200 1.5879543 28.90794 90-99
GGGCC 6012680 1.5796392 28.977053 2
GTTCT 2868875 1.5692331 24.416111 190-199
AGTTA 2623725 1.5659513 17.00716 50-59
ACAAT 2692505 1.563308 23.798767 180-189
TCTGA 3051290 1.5626515 21.667196 220-229
GCACT 3784195 1.5569464 26.736708 25-29
TTCTC 2711900 1.541256 23.246756 190-199
CGTCC 4354715 1.5373706 19.474306 180-189
CCATG 3725045 1.5326099 15.92393 160-169
GGCTT 3621190 1.5315224 22.162754 25-29
AGTGA 3314390 1.5295364 88.705315 9
TTAGT 2398000 1.528644 26.590137 110-119
GAGCA 4119620 1.5273367 7.8917437 200-209
CGGCC 5592030 1.5264568 32.15121 230-239
GTTAC 2943085 1.5072366 16.757475 50-59
CTTTA 2269460 1.5031589 43.685776 45-49
CATCA 3000875 1.4950464 32.95552 45-49
GTCCG 4377495 1.4873706 34.35574 70-79
TAGCA 3009130 1.4428542 12.399888 130-139
ACTCC 3371790 1.4414048 17.27258 140-149
CTGGT 3396650 1.4365572 32.676495 35-39
GTGAC 3613170 1.4307485 15.974663 210-219
CTCAA 2867285 1.4284914 19.129238 150-159
TACGC 3470645 1.4279413 25.156164 140-149
GTCCA 3425560 1.4093918 29.45209 180-189
AAGCT 2931765 1.405758 17.678665 200-209
CAACT 2818680 1.404276 18.61082 130-139
GGGGT 4455185 1.4021962 120.3765 7
GGCAG 4575875 1.4010231 13.77217 150-159
CAACC 3495515 1.3990699 15.744833 140-149
AACCC 3443330 1.3781826 21.060982 140-149
AGCAG 3702925 1.3728483 13.080918 50-59
CGTGT 3245895 1.3727978 24.925552 230-239
ATCTA 2199970 1.3642751 21.674866 110-119
GAGAG 3822485 1.3639492 26.393965 220-229
CTACG 3304210 1.3594643 16.727566 130-139
GAACT 2827760 1.3558885 14.94073 190-199
CCATA 2710515 1.3503879 15.798184 160-169
CACGT 3273980 1.3470266 16.919813 180-189
CGACA 3493110 1.3455977 16.60759 70-79
TTACA 2164250 1.342124 10.19289 120-129
GGCCT 3931375 1.3357894 69.98633 3
TCAAG 2782245 1.3340646 24.250376 150-159
CAATT 2150100 1.3333491 24.839462 180-189
ACAGT 2767980 1.3272246 12.850356 120-129
CCTTT 2328955 1.3236165 49.769627 45-49
GTGCG 4039130 1.320858 17.649473 240-247
TGGAA 2858445 1.3191252 19.607225 120-129
AAGAG 3034025 1.3109258 19.909805 150-159
TGTCC 2971445 1.3057649 55.01542 15-19
GTGGC 3988315 1.3042407 14.875519 40-44
ATACA 2243765 1.3027629 22.39739 35-39
TGCGA 3282545 1.2998271 14.863033 240-247
AATTC 2094540 1.2988945 24.80217 180-189
AACCA 2780865 1.2971476 17.950312 190-199
CCAGT 3152290 1.296959 19.644665 190-199
ACATG 2703965 1.2965298 14.966189 190-199
TGTGT 2460050 1.2950737 15.394887 240-247
ACAGA 2882345 1.293988 20.556938 140-149
ACATA 2217655 1.287603 21.777912 130-139
ATATT 1656210 1.2784364 27.169275 240-247
TCACT 2397945 1.2759778 13.740405 190-199
TTATT 1547630 1.2759335 20.235542 240-247
AGAGC 3436125 1.273933 25.272219 220-229
TCCGT 2894725 1.2720513 13.81232 150-159
GATTG 2580540 1.2719322 24.95485 100-109
ACGTC 3047185 1.2537155 18.765696 180-189
AGACC 3240280 1.2482036 102.48724 9
ATCCG 3021505 1.2431499 24.587072 70-79
CCTAC 2891105 1.2359169 17.476662 190-199
AGTCG 3114440 1.2332605 16.88817 150-159
ACACA 2640240 1.2315526 7.7983775 130-139
ATTAT 1593545 1.230065 9.555195 50-59
CTTCT 2153100 1.2236727 23.23648 30-34
CGGCT 3595830 1.2217791 21.32581 230-239
TGGAC 3058975 1.2112974 16.226282 80-89
TACCA 2412740 1.2020354 11.21981 45-49
ATTAG 2009390 1.1992899 26.270292 110-119
GTCGA 3024650 1.1977055 13.186111 150-159
TGAGT 2420015 1.1928104 21.416939 90-99
GATCC 2877845 1.184043 24.40845 70-79
TCGGA 2969985 1.1760591 97.3031 5
CTTCG 2673215 1.1747115 108.82967 3
ACGCA 3048025 1.1741444 21.619879 140-149
ATCAT 1885985 1.1695627 15.883206 110-119
GAAGT 2514315 1.1603148 13.823571 140-149
AGCTC 2818040 1.1594373 12.731146 210-219
ATTCC 2173635 1.1566194 22.720636 180-189
GGACT 2914750 1.154187 13.606859 90-99
GTATA 1929880 1.151835 10.681036 240-247
TCGAG 2906225 1.1508113 12.837262 150-159
GGGTG 3647750 1.1480693 14.750132 60-69
AAGGC 3089610 1.1454636 29.350304 25-29
TTGGG 2805860 1.1421227 16.140984 100-109
CGCTG 3353360 1.1393934 12.644203 190-199
GATCT 2211190 1.1324126 9.545521 220-229
GAACC 2915490 1.1230898 11.127016 180-189
AGGCC 3522550 1.1206075 14.264285 2
TTCGG 2647780 1.1198348 104.81294 4
ACAAG 2484635 1.1154418 17.986027 80-89
GCAAG 3001275 1.1127138 16.926678 25-29
GAAGC 2991255 1.1089988 13.765002 200-209
GCCCC 3909535 1.1088312 12.425374 80-89
GACCC 3347375 1.1064352 43.095264 10-14
CCCCT 3012360 1.104971 10.129061 140-149
TAGAC 2298200 1.1019688 18.408978 170-179
CGCAG 3455710 1.099344 16.903992 140-149
CACAT 2205505 1.0987902 14.450022 130-139
GCTTC 2495185 1.0964785 28.587402 2
CTTGA 2135860 1.093834 22.56965 90-99
AGGTC 2758520 1.0923228 18.280552 10-14
TTGAG 2211655 1.0901111 21.135088 90-99
GCACC 3282170 1.0848825 11.338273 130-139
CCGAT 2634835 1.0840605 23.524456 160-169
ATCAA 1846820 1.0722909 12.830883 110-119
ATGGG 2803625 1.0684878 20.126274 100-109
CTATA 1720890 1.0671816 13.765465 50-59
ATGAG 2299825 1.0613314 20.780088 60-69
GGAGA 2963665 1.0575027 87.30398 7
TAGTT 1651940 1.0530559 15.821168 50-59
TGGCT 2484420 1.0507443 32.65662 40-44
TACAG 2188220 1.0492343 9.411644 120-129
GAGCC 3287230 1.0457466 18.45312 220-229
GCTCT 2377600 1.0448071 17.991125 210-219
ACACC 2609215 1.0443308 13.113901 40-44
AGATC 2176575 1.0436504 12.985193 210-219
ATGGT 2115305 1.0426209 9.911187 35-39
CTCCG 2942260 1.0387232 9.891498 140-149
CAGCA 2685505 1.0344963 12.65356 45-49
GTAGC 2610190 1.0335869 11.460099 50-59
GGGAG 3484280 1.0267363 13.636315 5
CGTGA 2578045 1.0208582 20.816883 150-159
ACCGC 3087535 1.0205482 11.327114 220-229
GACAG 2745730 1.0179712 16.024601 70-79
CGAGG 3315005 1.014975 15.067735 220-229
AAACT 1743000 1.0120114 17.73999 130-139
AGCCG 3163995 1.0065426 19.463741 220-229
GTAGA 2174835 1.0036504 18.07841 170-179
TTTAG 1574310 1.0035694 31.595282 45-49
ACCAA 2139400 0.9979334 18.03293 130-139
TGAGG 2617600 0.99759185 11.959694 220-229
CGAGT 2504630 0.99178714 15.31687 160-169
TTTCA 1496870 0.99144006 7.4430957 100-109
GCCAA 2556760 0.9849017 15.035028 180-189
ATGAC 2053215 0.98450035 11.306571 160-169
ACTCA 1971270 0.9820936 12.687252 190-199
AACTC 1970815 0.9818668 16.594843 190-199
AAGGT 2123955 0.98017013 20.728853 15-19
CCAAC 2438035 0.9758163 14.8122425 130-139
GGTAT 1972645 0.9723046 9.497709 110-119
TTATA 1255815 0.9693696 13.1968565 110-119
CCGAG 3029275 0.96368486 18.386078 220-229
GGGAC 3117270 0.95443314 10.450406 90-99
CATAC 1915165 0.95414186 14.731183 130-139
CGGAC 2967170 0.9439279 11.096812 220-229
CCGTC 2662365 0.9399103 10.238206 140-149
CAGAA 2089980 0.938267 18.573406 140-149
GCCAT 2279640 0.9379212 7.9256816 60-69
AGCCC 2833145 0.9364625 11.745558 80-89
ACAGG 2522030 0.93503517 15.562243 70-79
AATCA 1608165 0.9337242 14.421182 110-119
GATAC 1942505 0.9314157 24.013391 35-39
ATGAT 1552355 0.9265119 8.807605 120-129
TATGG 1877370 0.92534405 12.738972 35-39
AACTG 1928230 0.92457104 12.025487 60-69
TAACA 1591045 0.9237841 8.922572 120-129
GCAGT 2320395 0.9188335 6.206823 50-59
TACAC 1836755 0.9150777 22.563784 35-39
TCATA 1469635 0.9113699 8.9866495 110-119
TGGTT 1728710 0.9100656 9.509547 50-59
CCCAG 2752420 0.90977985 15.229353 80-89
AAGTT 1524270 0.90974957 17.905626 140-149
GAGAT 1959335 0.90420073 12.505421 210-219
CCCCA 2623920 0.9011508 17.293581 80-89
GACTG 2267625 0.8979375 13.264664 90-99
TTTAT 1077935 0.88869643 10.593172 240-247
CGGAG 2880545 0.8819538 75.15598 6
GGTAG 2307405 0.87937367 12.50306 120-129
GTTAT 1377345 0.87801087 10.680059 110-119
CGTAT 1709030 0.8752423 7.820671 240-247
AGAAG 2021575 0.8734717 16.337984 140-149
TCCTT 1536475 0.8732259 10.773353 1
ATAGT 1432955 0.85524887 15.939031 120-129
TATTT 1036615 0.85463053 10.56631 240-247
GCCTA 2062720 0.8486731 16.877787 190-199
TTACC 1592170 0.8472144 9.970823 45-49
ATCCA 1700160 0.84702563 10.070177 180-189
GATCA 1758270 0.8430765 9.305252 110-119
CATGG 2125675 0.841728 8.700786 200-209
ACGCT 2038935 0.83888716 15.583602 190-199
GATGA 1812435 0.83640885 6.7169557 60-69
GTTTC 1528500 0.8360674 5.1268916 100-109
GGTTC 1962935 0.830191 11.764748 160-169
TCTTC 1458930 0.8291547 47.4894 2
AGTCT 1614075 0.8266132 15.0398035 8
GTCTA 1612320 0.82571447 10.343839 30-34
GCTTG 1936315 0.8189324 17.290524 90-99
CTATC 1536320 0.8174959 8.193219 110-119
ACAAA 1487420 0.8085828 13.33042 130-139
CCTCC 2202230 0.8078053 11.048068 140-149
TCATC 1516310 0.8068483 10.4464245 110-119
GGTGC 2466725 0.8066573 11.830737 60-69
GTCTG 1906150 0.80617464 14.095112 1
ACATC 1611635 0.80292207 5.9216943 110-119
ATATG 1343520 0.8018702 5.975102 50-59
TGGCA 2022030 0.8006864 5.6105514 100-109
ATATA 1097415 0.79311734 10.982645 130-139
TATAC 1265980 0.7850766 9.153156 130-139
TTATG 1220805 0.7782219 11.400577 50-59
GTTCA 1507600 0.77208436 14.366667 160-169
CATTA 1242925 0.7707795 12.569516 110-119
TGATG 1529680 0.7539698 11.81243 120-129
GGTTA 1528270 0.75327486 8.463902 50-59
CCAGC 2262065 0.7476988 6.225878 80-89
CCCCC 2536930 0.7476081 13.785784 80-89
GGGAT 1947110 0.74206185 13.584958 100-109
GCGGA 2417635 0.74022174 9.436351 220-229
CTGAC 1798175 0.7398302 9.670612 220-229
GACCG 2325295 0.73973197 9.775755 210-219
TCAAC 1474645 0.73467326 7.7066565 110-119
CATCC 1697780 0.7257831 6.681523 110-119
TGACT 1412380 0.72331953 5.0512695 2
AACGC 1867380 0.71934235 14.68617 180-189
ATCTT 1083595 0.7177107 13.507183 3
TTGGA 1454390 0.7168598 6.518466 60-69
TTTAC 1080515 0.7156706 13.554709 45-49
ATGCC 1738065 0.715099 8.478926 50-59
TCCGG 2102965 0.71453846 12.111052 70-79
TGCCA 1730565 0.71201324 7.6227293 60-69
GATTA 1190555 0.71057415 5.6100664 160-169
ACGCC 2146380 0.70946056 13.077637 180-189
GGGCA 2312975 0.7081774 13.770014 150-159
ATAAA 1041090 0.7044621 7.755731 130-139
TATTG 1101780 0.7023475 6.3986745 240-247
GACCA 1823145 0.7023024 6.2026477 170-179
CAACG 1820710 0.7013644 14.959112 180-189
CGCCG 2553980 0.6971602 11.119585 220-229
GAAAT 1246190 0.6963811 7.4996653 110-119
GGATA 1498440 0.6915054 15.457915 35-39
CACAA 1482345 0.69144696 8.440413 130-139
TATAG 1155135 0.689434 6.415991 120-129
CAAAC 1477980 0.68941087 14.239644 130-139
AATAC 1173710 0.6814733 11.396127 130-139
TTTCT 952390 0.67374325 9.651637 4
GCCGC 2430805 0.663537 12.222394 220-229
CACGC 1965260 0.6495935 12.565114 190-199
GCGAC 2041465 0.6494389 7.240428 70-79
AAATA 958545 0.6486074 12.63022 130-139
AGGCA 1742430 0.6460007 6.446496 80-89
CAGTC 1564000 0.64348274 5.367506 210-219
CCCGT 1812355 0.63982624 10.057635 140-149
GACGA 1723145 0.6388508 9.96586 220-229
TACTT 959055 0.6352226 12.121822 1
TCCAC 1469770 0.6283112 10.452757 180-189
CCGGC 2291450 0.6254973 9.788754 70-79
ACTTC 1172960 0.6241473 29.666956 4
TCAAT 1005445 0.62351024 8.666317 110-119
AGATG 1346325 0.6213068 5.8242335 120-129
AAGCA 1363390 0.61207455 6.0199385 120-129
TAGCT 1194180 0.6115732 12.706693 50-59
CACGA 1571330 0.6052996 9.02841 180-189
AATAA 891565 0.6032848 7.2123384 130-139
CCCTA 1407220 0.6015717 11.978141 110-119
CACCG 1814700 0.59982765 9.153372 45-49
AATGG 1284380 0.5927202 6.8195705 120-129
GGCCC 2161650 0.5900658 10.37199 70-79
TTAGC 1146925 0.58737254 10.242998 45-49
ACGAC 1510535 0.5818804 9.286992 220-229
AGTAA 1037185 0.5795874 5.085893 50-59
CCGTA 1394915 0.5739154 6.7529273 230-239
CTTTG 1047510 0.57297283 6.9639945 45-49
CGGTT 1342475 0.5677776 11.653242 160-169
ACCCG 1716135 0.56724817 8.807759 140-149
GTTCC 1284160 0.5643083 6.7898364 140-149
ACCTA 1130940 0.56343824 7.1179 130-139
CGGCA 1761585 0.56040233 11.270535 70-79
TGATT 878515 0.5600236 6.0065117 50-59
GGGGA 1890240 0.5570097 8.806254 4
ATTTC 836335 0.55393994 6.2060947 240-247
GGTGA 1439580 0.5486374 6.316248 120-129
TAATA 756995 0.547091 7.924394 130-139
TCAGG 1381355 0.54699105 5.4312353 150-159
CTGCG 1599945 0.543624 11.939406 25-29
TTCAA 868235 0.53842163 5.341582 200-209
ATTAA 742945 0.5369368 6.4148426 110-119
CCACG 1618375 0.5349347 7.867218 180-189
AAATC 914945 0.53123057 8.099202 110-119
ACCCC 1542035 0.5295916 9.571107 140-149
GCTAC 1279855 0.52657574 8.0151825 50-59
CCTAT 988990 0.5262544 5.195121 110-119
CTGTT 951935 0.5206947 5.9229455 240-247
TATGT 808165 0.51517785 6.755305 140-149
GTCCT 1171085 0.51461893 6.439694 45-49
GAAGA 1181150 0.51034516 6.1135154 15-19
AAGAT 911700 0.5094654 5.60953 15-19
ACATT 813530 0.50449723 6.277713 110-119
GGGTA 1315825 0.50147325 5.573316 100-109
TGACG 1238000 0.49022508 8.803626 220-229
GTAAC 1017215 0.4877465 7.1439023 120-129
AGTTT 761250 0.48527116 5.080589 140-149
CGCGG 1839895 0.48337352 6.585133 220-229
GGGTT 1169025 0.47585058 5.7913437 100-109
AGTAT 786920 0.46966752 5.1179867 110-119
TATCC 879075 0.46776727 5.3875213 110-119
TGGCC 1375400 0.46732882 14.907377 2
CCGGT 1371820 0.46611243 9.043742 160-169
CCATT 862660 0.45903262 5.0943217 110-119
CATAA 786460 0.45663026 6.1749964 110-119
CAATC 915340 0.45602554 6.4760766 110-119
AACAT 781615 0.45381713 5.9841313 110-119
GGTTT 856860 0.45108712 5.4883184 100-109
ATGCT 868280 0.44467065 7.019589 50-59
CCGCG 1628310 0.44447994 6.7912188 220-229
TAAAT 606525 0.43834418 7.7325115 130-139
TGCTA 853310 0.4370041 6.250867 50-59
TGCGC 1247670 0.42392915 8.934133 25-29
ATTCT 638115 0.4226505 7.2315693 2
TTGGT 802680 0.42256448 5.286649 120-129
GGTCA 1054710 0.41764566 5.2881775 160-169
GCATG 1053955 0.4173467 8.457103 60-69
GCCTT 935775 0.41121483 8.719238 1
GCGCT 1208505 0.4106218 8.150849 25-29
ACGAG 1090855 0.40443125 7.928314 180-189
CTAAC 804610 0.4008595 5.6690207 110-119
ATGGC 992550 0.39303145 7.5674195 1
CCTAA 785625 0.39140105 7.7343407 110-119
CACTT 732360 0.38969833 6.9332304 3
CAATG 807540 0.38720903 5.096629 120-129
TACCT 726415 0.3865349 5.1484804 2
AGCTT 754230 0.3862624 7.4982758 1
GGTAA 831740 0.38383433 7.4880486 120-129
CCGGG 1443000 0.37910205 5.1676564 80-89
CATGC 916220 0.37696403 6.8592725 60-69
TTTGA 585790 0.3734213 5.902471 45-49
GAGGC 1203090 0.36835724 5.9119973 35-39
TTCTT 505685 0.35773355 20.367567 1
CGAGC 1063075 0.33818963 6.6680503 180-189
TGGGC 1032490 0.3376402 9.484237 1
TGCTG 784830 0.33193088 14.054362 1
ATGTG 662440 0.3265126 5.0968385 140-149
AACCT 617165 0.3074737 5.189623 2
CTCTT 540565 0.30721968 9.452834 1
CGCTT 682275 0.29981738 5.9036565 1
GCGAG 974920 0.2984971 5.469175 240-247
GCTTT 543180 0.2971116 5.022119 45-49
AACTT 463100 0.28718382 10.68691 3
GACTT 554650 0.28405187 7.025719 1
TTCCT 488100 0.2774022 6.325306 4
CTCGG 806520 0.27403668 5.2073364 6
ATTGT 419950 0.26770392 5.2586102 240-247
GAGGT 684430 0.26084268 6.236972 7
CCCTT 562370 0.25677025 5.482144 3
TAACT 403855 0.25044402 6.8488603 2
GTCTT 456760 0.24984112 11.828838 1
TTGGC 582640 0.24641795 7.103978 1
TGTAA 410875 0.24522777 7.531294 25-29
AATCT 392085 0.24314505 7.8469663 2
CGGGG 936545 0.23680627 8.662096 3
CCTCG 638170 0.22529687 5.684907 5
TGCTT 385720 0.21098326 11.723435 1
TCGGT 452835 0.1915191 6.22083 7
TGCCT 414750 0.1822568 5.2218943 4
TCGCA 372115 0.15310076 5.276611 7
TAGGC 379730 0.15036605 7.0381746 1
TTGCT 218255 0.11938232 5.6938295 4
Produced by FastQC (version 0.10.1)