FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_2_SL248348.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_2_SL248348.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13935984
Filtered Sequences 0
Sequence length 251
%GC 55

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 60701 0.43557024749741385 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 52142 0.374153701669003 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 51906 0.3724602439268013 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 46853 0.3362015915058456 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 44307 0.31793233976158414 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 44007 0.3157796392418361 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 42054 0.3017655588582765 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 41675 0.29904598053499487 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 40462 0.29034189476681377 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 40133 0.2879810998634901 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 39089 0.28048970205476703 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 38932 0.27936312211609887 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 38928 0.2793344194425022 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 38558 0.276679422134813 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 37712 0.2706088066691236 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 37165 0.2666837160547831 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 36970 0.26528446071694683 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 36585 0.26252182838327026 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 36487 0.26181861288015257 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 35170 0.25236825759845877 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 35082 0.2517367987793327 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 34978 0.25099052926582005 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 34819 0.2498495979903536 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 34693 0.24894546377205945 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 33315 0.2390573927180169 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 31760 0.227899228357323 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 31447 0.22565324414838595 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30806 0.22105364070452432 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30806 0.22105364070452432 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29905 0.21458836347688115 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 29865 0.21430133674091473 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 29506 0.2117252717856163 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 28254 0.20274133494986793 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 28222 0.20251171356109476 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27990 0.20084695849248965 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27306 0.1959388013074642 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 27056 0.19414488420767417 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26838 0.1925805884966573 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 26802 0.19232226443428754 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25674 0.18422811048003498 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 25493 0.18292931449978703 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 25485 0.18287190915259374 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25059 0.17981507441455158 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24897 0.17865261613388764 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24406 0.17512936294990006 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24269 0.17414629637921514 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23593 0.16929554454138293 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23502 0.16864255871705938 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22914 0.16442326569835328 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 22561 0.16189025475344979 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 22125 0.158761663331416 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 22081 0.15844593392185294 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21921 0.15729782697798733 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21707 0.1557622339405671 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 21232 0.15235379145096606 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20799 0.14924672703412978 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 20407 0.14643386502165903 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 20321 0.14581675753933127 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 19635 0.1408942490175075 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 18518 0.13287902741564572 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 17889 0.12836553199257406 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 16972 0.12178544407054429 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 16696 0.11980495959237611 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 16315 0.11707102993229614 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15798 0.1133612093699304 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 15299 0.10978055083874952 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14735 0.10573347386162327 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14581 0.10462842092815261 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 14015 0.10056699261422804 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 28615545 7.735581 69.10011 35-39
TATTA 14244755 7.5031204 131.04588 240-247
TGGAT 21503640 7.2056494 82.41967 35-39
TCACC 22263105 6.5615215 76.64816 160-169
TCTCC 19887045 6.246426 89.34585 20-24
CTGTG 20916175 6.025814 91.04469 25-29
TCCCT 18649040 5.8575745 73.48558 15-19
AGTGG 22241150 5.7581663 40.52231 100-109
CTGAG 21259600 5.7470636 52.595272 15-19
TGTAT 13123155 5.69159 60.765923 240-247
TTACT 11865385 5.3732996 113.765175 240-247
ATTAC 11926010 5.0677114 108.471115 240-247
CCTGA 17802035 5.024866 54.32657 15-19
TGGGT 18123925 5.0005965 47.141663 100-109
TTCAC 13456630 4.916152 79.231995 40-44
CTCTG 15968565 4.803561 76.192535 35-39
CTCCA 16273710 4.7962894 43.83267 80-89
ACACG 17793515 4.7127466 51.17088 230-239
TGAGA 14806985 4.6557026 99.87203 15-19
GACAC 17462910 4.6251836 48.141087 230-239
ATTCA 10795725 4.58742 119.41528 40-44
AAGGG 18199530 4.421245 40.401684 90-99
TCTGG 15054025 4.336967 138.34906 35-39
TACTG 12388930 4.3346987 80.09109 240-247
AGCCT 15226395 4.2978573 54.689373 30-34
GAAGG 17527460 4.257978 33.061527 150-159
CAGCC 18572180 4.229096 59.30394 30-34
CCAGG 19367970 4.223814 55.26631 80-89
GAGAC 16564885 4.2018137 85.665985 8
ACCAT 12233510 4.193702 69.469894 160-169
CCCTG 17275125 4.1922617 87.584076 10-14
TCCAG 14716340 4.153887 43.0824 80-89
TGGAG 16001935 4.142853 39.761436 90-99
GGGTC 18333090 4.080703 132.97662 8
ACTAC 11869995 4.069087 36.372814 130-139
TCAGT 11592205 4.055937 92.950386 7
TACTA 9497705 4.035854 31.590057 130-139
CACCA 14489190 4.007009 58.971874 160-169
GTATT 9172480 3.978159 70.30116 240-247
GACTC 13939410 3.9345882 54.63979 20-24
AGACT 11958300 3.9260151 73.67222 15-19
GATTC 11147340 3.900285 62.136486 40-44
CTGGG 17390850 3.8709726 140.07883 3
ACTGT 10928350 3.8236637 68.53277 240-247
CACCT 12973380 3.8235953 45.074596 40-44
TGAAG 12043150 3.7866812 42.340813 150-159
CAAGA 12137000 3.7389646 46.368195 180-189
GGACA 14332520 3.6355567 28.927485 220-229
GCTGG 16330375 3.634925 35.17094 2
ACTCT 9850950 3.598878 72.15149 20-24
ACTGG 13145205 3.553516 44.521843 60-69
CCTGT 11803885 3.5507689 63.55249 25-29
TATCT 7626740 3.4538078 52.05368 200-209
TCCTG 11227605 3.377416 64.70317 20-24
GGATT 10062500 3.3718407 59.264668 40-44
AGACA 10936015 3.368985 39.30173 170-179
GAGTG 12986250 3.3621008 39.314316 100-109
GGTCC 14357970 3.3370006 148.86162 9
CTCCT 10565225 3.3184867 96.09704 20-24
AGCTG 12011850 3.2471387 37.472637 60-69
CATCT 8858035 3.2361333 33.967094 170-179
CTGCA 11449330 3.231729 44.913612 25-29
CCATC 10644475 3.1372063 35.29596 45-49
GTCTC 10390480 3.1255975 33.332283 30-34
GTCCC 12865315 3.1221056 78.69928 10-14
AAGAA 8543095 3.0611382 39.65924 180-189
AGTAG 9655865 3.0360563 30.403719 50-59
GGAGT 11708630 3.031329 38.164402 90-99
GGCTG 13534265 3.0125482 35.990025 90-99
CCTCA 10084420 2.9721434 78.861946 5
AGGGA 11985275 2.911605 42.21657 80-89
TGTGC 9961215 2.8697612 60.485836 25-29
GAACA 9302945 2.865896 37.333733 210-219
GAGCT 10598200 2.8649895 30.927458 60-69
GTGAA 8998640 2.8294072 41.150543 150-159
GACAA 9139950 2.8156836 26.096682 180-189
ATCTG 8044300 2.8145785 38.745438 200-209
GGAAG 11566375 2.8098407 31.634672 80-89
ATGAA 7345660 2.8050528 29.179592 210-219
ATCTC 7654780 2.7965448 50.436077 170-179
CAGGG 13257950 2.7690647 37.82031 80-89
ACCTT 7538475 2.7540545 72.602135 40-44
CTATG 7726440 2.7033641 28.277882 50-59
GTGTA 8013130 2.6851182 36.116318 230-239
CGCCA 11625395 2.6472337 41.631954 70-79
AGAAC 8591700 2.6467876 31.8476 190-199
GGGAA 10863235 2.6390257 35.09376 80-89
CACGG 11913355 2.5980935 45.46272 230-239
TGCAA 7912540 2.5977566 37.90306 200-209
GAGTC 9392130 2.538955 27.484007 150-159
CCAAG 9565095 2.5333874 40.555233 180-189
AGGGC 12125955 2.5326355 33.682224 150-159
AGGCT 9360495 2.5304034 21.177439 80-89
ACGGC 11381245 2.4820495 50.04087 230-239
CCCTC 9756710 2.4722621 26.99008 20-24
CAGTG 9101175 2.4603016 71.74471 8
TATAT 4666435 2.4579449 25.705301 240-247
TGAAC 7478650 2.4553068 39.904305 210-219
CTACA 7134855 2.4458604 18.987011 190-199
ATCAG 7433470 2.4404736 27.37805 45-49
CAAAT 6112675 2.4372802 38.298885 200-209
CTCTC 7738745 2.430703 102.926025 20-24
CACTG 8607585 2.4296079 19.820532 30-34
GCAGC 11119275 2.4249184 36.376293 30-34
TCTGT 6433925 2.3990743 24.383656 210-219
CCGCC 12232165 2.3947506 35.73095 70-79
CTTCA 6485380 2.3693242 52.003773 45-49
ACAGC 8920820 2.3627465 28.9875 210-219
GTGGA 9115730 2.360035 26.116919 100-109
CTACT 6447560 2.3555074 22.50451 50-59
GGGCT 10580165 2.3550045 43.630127 90-99
GTATC 6723685 2.3525152 24.304487 200-209
AAATG 6130515 2.341031 36.24258 200-209
CTGTC 7776150 2.3391714 48.39108 30-34
AATGA 6082715 2.322778 27.389141 210-219
GGCCG 12867765 2.3106394 25.146536 230-239
TCCAA 6720055 2.303665 44.22338 180-189
TATGC 6563500 2.2964687 21.286062 50-59
CAGGC 10511555 2.2923853 33.96215 70-79
ACAAC 7096030 2.2825449 21.58587 180-189
GTGGT 8237795 2.272901 32.536514 120-129
GCCTC 9337090 2.2658894 68.85436 4
GCTCC 9319895 2.2617166 35.788162 80-89
GTAGT 6715375 2.2502534 27.602022 120-129
GGGGG 13600545 2.2400491 100.12332 5
CAGTA 6807595 2.2349935 22.467354 45-49
TGGTG 8060260 2.2239172 33.911846 35-39
GCCTG 9568550 2.2238698 31.494686 210-219
TACAT 5159370 2.1923676 17.922392 190-199
CCTCT 6861110 2.155042 51.50441 35-39
GCTAT 6153285 2.152941 34.49098 50-59
TGCAG 7956600 2.150891 45.989407 30-34
GTGGG 10083295 2.149502 31.3693 100-109
GCTGT 7445355 2.1449585 20.126507 230-239
CCTTC 6772155 2.1271017 56.82177 45-49
AACAG 6887630 2.1218262 35.977345 210-219
GCAGA 8296380 2.1044421 26.028555 140-149
TATCA 4914295 2.088228 17.494678 170-179
TCTAT 4548730 2.0599158 19.740314 110-119
CAGAG 8113965 2.0581713 34.51454 170-179
TCCGC 8359005 2.0285316 44.099506 70-79
GGCTC 8710330 2.0244071 19.826508 40-44
AACTA 5059190 2.0172286 36.06996 130-139
CTGTA 5749420 2.0116348 26.334595 190-199
TGAGC 7428255 2.0080647 17.430405 200-209
CTCAC 6794260 2.002447 54.083553 20-24
AGCAC 7537155 1.9962724 21.72914 130-139
TGCAC 7048185 1.989446 26.646555 25-29
TGGTA 5932530 1.9879303 32.011124 120-129
TGTGA 5909455 1.9801979 18.684673 210-219
CATAT 4647665 1.9749292 14.318213 160-169
CAGAC 7382140 1.9552155 28.371828 140-149
ATATC 4598565 1.9540653 13.714537 170-179
TTCAG 5583485 1.9535764 48.480034 45-49
CCGTG 8402790 1.9529306 19.661966 230-239
ATGGA 6203550 1.9505581 12.195109 120-129
GGTCT 6739415 1.9415816 33.95033 15-19
GCCAG 8900495 1.9410417 36.965603 70-79
CCAGA 7322190 1.9393373 36.331837 170-179
AGAGA 6552925 1.9333534 30.163898 170-179
TAGTA 4733980 1.926545 18.964804 120-129
TGGGG 9032360 1.9254694 130.05928 4
AGAGT 6096270 1.9168265 30.17042 150-159
ATACT 4483670 1.9052429 29.329529 130-139
CTATT 4206210 1.9048038 20.56431 110-119
AGGAC 7473430 1.8956945 21.619974 220-229
TCTCT 4859865 1.8921511 33.12565 35-39
GCCGA 8641180 1.8844894 20.898808 220-229
ACTAT 4422520 1.8792585 17.216751 130-139
GCACA 7076120 1.8741637 19.137457 130-139
GGTGG 8688785 1.8522279 18.246714 40-44
TCCAT 5067585 1.8513566 27.139877 45-49
CAGTT 5275695 1.8458854 24.692026 190-199
GAGGA 7558720 1.8362538 28.22062 220-229
TTCCA 5013920 1.8317509 24.316015 180-189
TAGTG 5450920 1.8265473 27.317598 120-129
GGATC 6752850 1.8254839 18.24492 70-79
ACCAG 6886510 1.8239441 14.138233 190-199
ACCCT 6184315 1.8226798 42.579155 10-14
GTCAC 6446500 1.8196123 37.138977 160-169
GATGG 6985160 1.8084368 20.087254 100-109
GCAAA 5869375 1.8081391 34.42207 200-209
TGGGA 6954065 1.8003864 16.038425 100-109
CATGA 5456505 1.7914187 25.619997 60-69
CCTGC 7363915 1.7870468 47.79907 20-24
TGTCT 4789590 1.7859364 49.570866 30-34
GCTGA 6593880 1.7825099 26.347809 200-209
GTGCA 6543455 1.7688787 53.63817 25-29
TCAGC 6227550 1.7578107 11.385741 45-49
CACAG 6617120 1.7525941 15.419894 140-149
AGCTA 5280030 1.7334806 26.864113 50-59
TACAA 4346505 1.7330629 17.707254 130-139
AACAC 5378940 1.7302171 24.099884 190-199
AGTCA 5263735 1.7281306 28.643852 160-169
ACCTG 6061010 1.7108024 30.378183 25-29
CAAGG 6738055 1.7091608 16.860884 80-89
AGTTC 4878415 1.7068833 21.78216 190-199
CTCAG 5991315 1.69113 75.20055 6
TGACC 5973160 1.6860056 14.158277 210-219
TTCTG 4513205 1.6828783 40.931786 30-34
AGGGG 8407715 1.6817862 34.565872 90-99
CTGAA 5102970 1.67535 23.335224 200-209
TCTGC 5546435 1.668443 35.09061 200-209
CCTGG 7170615 1.6665549 36.234673 2
ATGCA 5064745 1.6628004 16.428295 60-69
GGGGC 9548460 1.6420958 29.923223 90-99
ATTGG 4899285 1.6417 20.28947 100-109
CGATT 4681275 1.6379069 36.23993 160-169
GGATG 6304040 1.6320969 21.65107 100-109
TCTCA 4461740 1.6300215 28.35163 100-109
GGAGC 7783505 1.6256684 12.541712 200-209
GGGCC 9003120 1.6166728 27.175447 2
ACAAT 4006265 1.5974005 24.594406 180-189
GCCGT 6800600 1.5805581 29.211367 230-239
AGTTA 3870315 1.5750673 16.372074 50-59
TTAGT 3631595 1.5750442 27.446304 110-119
CAGCT 5544100 1.5648975 13.0104885 50-59
GTCCG 6712295 1.5600348 36.02007 70-79
CTCCC 6024695 1.5266032 13.692866 190-199
GCACT 5396980 1.523371 25.596302 25-29
CCATG 5383000 1.5194249 15.259116 160-169
TCTGA 4320705 1.5117493 20.566214 220-229
AGTGA 4804240 1.5105785 83.18967 9
CGTCC 6210500 1.5071404 18.584858 180-189
GTTCT 4038515 1.5058764 22.840067 190-199
CTTTA 3321565 1.5041877 43.67549 45-49
ACTCC 5087535 1.49943 18.332521 140-149
CGGCC 7975120 1.4953065 31.499664 230-239
TTCTC 3816685 1.4859968 21.612692 190-199
TAGCA 4498440 1.4768775 12.457486 130-139
TACGC 5224790 1.4747679 26.245947 140-149
GTTAC 4188080 1.4653456 15.710991 50-59
GAGCA 5744225 1.457068 7.496457 200-209
GGCTT 5054565 1.4561874 20.845737 25-29
GGGGT 6813795 1.4525279 124.759224 7
CATCA 4198240 1.4391754 31.770704 45-49
GAGAG 5806695 1.410631 27.390446 220-229
CTCAA 4109385 1.4087156 18.962263 150-159
CTACG 4966100 1.4017493 16.823826 130-139
GAACT 4266480 1.400723 15.654723 190-199
CAACT 4081575 1.3991822 17.370733 130-139
GTGAC 5153435 1.3931173 14.895508 210-219
CTGGT 4817895 1.3880042 31.163565 35-39
CAATT 3253085 1.3823313 25.670532 180-189
GGCAG 6515505 1.360833 13.213886 150-159
AAGCT 4141625 1.3597319 16.72962 200-209
CAACC 4908915 1.3575685 15.600184 140-149
GTCCA 4790560 1.3522006 28.034643 180-189
AGCAG 5306315 1.3459889 13.381077 50-59
AATTC 3166595 1.3455791 25.63039 180-189
CCATA 3924750 1.3454219 14.772708 160-169
AACCC 4851955 1.3418161 20.336662 140-149
ACATA 3359590 1.3395547 23.05198 130-139
TTATT 2375880 1.3336873 21.293257 240-247
TCCGT 4428480 1.3321469 14.377552 150-159
CCTTT 3413160 1.3288876 50.397068 45-49
ATCTA 3124080 1.3275133 20.519672 110-119
AGAGC 5226040 1.3256263 26.402845 220-229
TGGAA 4210450 1.3238754 19.683907 120-129
TGTGT 3666935 1.3095042 15.53063 240-247
TCACT 3583345 1.3091147 14.644921 190-199
CACGT 4634105 1.3080391 16.71369 180-189
TCAAG 3975780 1.3052837 23.313189 150-159
CGTGT 4527370 1.3043059 23.266035 230-239
ATATT 2474400 1.3033373 27.467213 240-247
ATACA 3264375 1.30159 21.697197 35-39
CGACA 4907350 1.2997487 15.819526 70-79
GGCCT 5556095 1.2913171 65.79678 3
ACAGT 3926170 1.2889962 12.307749 120-129
ACATG 3906440 1.2825186 14.079675 190-199
TTACA 3016620 1.2818503 9.5967045 120-129
GTGGC 5754540 1.2808844 14.313099 40-44
TGTCC 4224140 1.2706786 53.228153 15-19
CCAGT 4491560 1.2678038 18.870726 190-199
AAGAG 4283740 1.2638606 18.917046 150-159
AACCA 3918935 1.2605845 17.79947 190-199
ACAGA 4066365 1.252698 19.672434 140-149
GTGCG 5621460 1.2512625 16.673376 240-247
ATTAG 3040720 1.2374543 27.099634 110-119
CGGCT 5321580 1.2368124 22.054207 230-239
TGCGA 4562605 1.2333995 14.016783 240-247
ATTAT 2336575 1.230741 9.1831665 50-59
AGACC 4613975 1.222046 97.23283 9
GATTG 3642770 1.2206551 23.518896 100-109
ATCCG 4320870 1.2196243 23.961584 70-79
CCTAC 4125720 1.215958 16.634296 190-199
ACGTC 4290445 1.2110364 18.459274 180-189
ACGCA 4570815 1.2106148 22.568913 140-149
AGTCG 4465880 1.207252 15.823652 150-159
ATTCC 3280940 1.1986359 23.721392 180-189
TACCA 3458915 1.1857314 10.459099 45-49
ACACA 3683480 1.1848469 7.7835507 130-139
TGGAC 4362110 1.1792002 15.270201 80-89
CTTCT 3017205 1.1747255 22.115917 30-34
GTCGA 4319315 1.1676314 12.318373 150-159
GATCC 4123765 1.1639887 23.80409 70-79
CGCTG 4986220 1.1588699 13.226786 190-199
GAAGT 3639375 1.1443145 13.136885 140-149
GGACT 4220255 1.1408528 12.816685 90-99
TGAGT 3402455 1.1401278 20.08669 90-99
CCGAT 4037675 1.1396887 24.698261 160-169
CTTCG 3783840 1.1382304 102.470215 3
CGCAG 5168530 1.1271657 17.77742 140-149
AGCTC 3989220 1.1260116 12.566324 210-219
ATCAT 2640750 1.1221322 15.29216 110-119
TCGGA 4146030 1.1207876 91.35041 5
TCGAG 4140010 1.1191603 11.962406 150-159
CACAT 3255930 1.1161474 15.362637 130-139
GGGTG 5235365 1.1160467 13.854318 60-69
GTATA 2738995 1.1146641 10.783957 240-247
ATGAG 3535050 1.111512 21.872757 60-69
TTGGG 4025510 1.1106838 15.055375 100-109
AAGGC 4348405 1.1030072 27.938274 25-29
GAACC 4161215 1.1021291 10.450955 180-189
GCAAG 4304455 1.091859 16.151575 25-29
CTCCG 4474490 1.0858523 10.669348 140-149
GAGCC 4972020 1.0843103 19.426886 220-229
GACCC 4759680 1.0838331 41.803097 10-14
TTCGG 3738970 1.077173 98.23787 4
GTAGC 3982855 1.076677 12.246794 50-59
AGGCC 4928810 1.0748869 13.669747 2
GATCT 3071180 1.0745592 8.94942 220-229
TAGAC 3265250 1.0720103 17.292498 170-179
CGTGA 3962020 1.0710447 21.751326 150-159
ACAAG 3471245 1.0693632 17.036398 80-89
GAAGC 4214360 1.0690056 13.024966 200-209
GCTTC 3535670 1.0635775 27.045025 2
GCCCC 5431385 1.0633286 12.155268 80-89
GCACC 4655650 1.0601443 11.284689 130-139
AGGTC 3899035 1.054018 17.536873 10-14
AGCCG 4829565 1.0532434 20.555202 220-229
CGAGG 5036055 1.051834 15.7883625 220-229
CTATA 2462235 1.046276 13.638967 50-59
ATCAA 2617830 1.043796 12.375758 110-119
CTTGA 2972600 1.0400677 21.25914 90-99
GCCAA 3921120 1.0385382 15.964441 180-189
TTGAG 3086435 1.0342326 19.81129 90-99
CCCCT 4072885 1.0320321 9.238741 140-149
ATGGG 3986000 1.0319633 19.07209 100-109
CAGCA 3869805 1.024947 12.154138 45-49
ACACC 3705470 1.0247538 12.755298 40-44
AACTC 2987495 1.0241265 17.49702 190-199
GGAGA 4211090 1.0230079 81.86936 7
TAGTT 2355830 1.0217373 14.942884 50-59
GCTCT 3378900 1.0164189 17.250847 210-219
TTTAG 2343415 1.0163529 32.14074 45-49
ACTCA 2957770 1.0139368 13.565548 190-199
TGGCT 3511105 1.0115266 31.015877 40-44
ATGGT 3015575 1.0104883 9.577813 35-39
GGGAG 5044145 1.0089749 13.691539 5
CCGAG 4621335 1.007832 19.368475 220-229
TTATA 1912095 1.0071552 13.974253 110-119
TTTCA 2222015 1.0062507 8.086352 100-109
TACAG 3056150 1.003361 8.775419 120-129
CATAC 2915320 0.99938464 15.561146 130-139
GACAG 3901775 0.9897162 15.177594 70-79
ACCGC 4340965 0.9884869 11.269861 220-229
TGAGG 3782295 0.97922474 11.623535 220-229
AAACT 2454660 0.978736 16.589375 130-139
AGATC 2965490 0.97359645 12.231582 210-219
ATGAC 2945490 0.9670303 10.781166 160-169
AACTG 2940675 0.9654495 12.787759 60-69
CCGTC 3976470 0.96499467 10.200994 140-149
GTAGA 3068205 0.96472377 17.008085 170-179
CGAGT 3565090 0.9637434 15.174378 160-169
TTTAT 1709710 0.9597364 11.54963 240-247
ACCAA 2940675 0.9459124 17.00498 130-139
GCCAT 3336815 0.9418614 8.16953 60-69
CCAAC 3404635 0.9415574 13.860841 130-139
ATGAT 2304815 0.93796974 8.977104 120-129
GGTAT 2790035 0.93491215 9.431911 110-119
GGGAC 4459830 0.93148327 9.833451 90-99
AAGGT 2961285 0.9311053 19.664677 15-19
TCATA 2186060 0.92892104 8.994979 110-119
GCAGT 3432895 0.92800736 6.5116806 50-59
GTTAT 2122480 0.9205321 11.220646 110-119
CGGAC 4219905 0.9202871 10.568275 220-229
TAACA 2293135 0.9143317 8.815975 120-129
AATCA 2289320 0.91281056 13.797911 110-119
TATGG 2722475 0.9122734 12.376529 35-39
GATAC 2778215 0.9121125 23.36575 35-39
TATTT 1623795 0.9115085 11.046522 240-247
AGCCC 3997245 0.91021806 11.55122 80-89
ACAGG 3573725 0.9065035 14.737738 70-79
CAGAA 2913815 0.8976395 17.592201 140-149
CCCAG 3927395 0.8943123 14.640258 80-89
GGTAG 3450865 0.8934185 12.863968 120-129
TACAC 2605645 0.8932267 21.779146 35-39
TGGTT 2479560 0.88547915 9.233042 50-59
GACTG 3254075 0.8796674 12.458704 90-99
ACGCT 3108515 0.8774206 16.319532 190-199
AAGTT 2150070 0.87499464 17.037868 140-149
CGTAT 2486345 0.8699344 7.880178 240-247
CCCCA 3649020 0.8676104 16.649723 80-89
GAGAT 2754080 0.86595464 11.785341 210-219
GATGA 2724235 0.8565706 7.056059 60-69
CGGAG 4092255 0.85471123 70.56546 6
GTTTC 2281305 0.8506502 5.6878285 100-109
ATAGT 2081255 0.8469896 15.697206 120-129
GGTTC 2928065 0.8435565 12.0925045 160-169
TCCTT 2164300 0.8426536 10.687318 1
TCTTC 2158270 0.84030586 44.02385 2
AGAAG 2821085 0.8323236 15.393307 140-149
AGTCT 2371205 0.8296486 14.325631 8
ATATA 1670540 0.8256601 11.9303465 130-139
GTCTG 2863885 0.8250666 14.81584 1
GTCTA 2347040 0.82119364 10.003245 30-34
TTACC 2245685 0.820423 9.35453 45-49
TGGCA 3001680 0.81143796 5.762408 100-109
ATCCA 2356290 0.8077467 9.326009 180-189
CATGG 2980965 0.80583805 8.1633415 200-209
TATAC 1895320 0.80537707 9.965761 130-139
GCCTA 2848715 0.80408853 15.922155 190-199
TTATG 1847155 0.80112207 11.892087 50-59
AAAAA 1831990 0.7972278 5.3738403 190-199
GTTCA 2277455 0.7968469 14.767421 160-169
GATCA 2414410 0.79267204 8.7464075 110-119
CATTA 1863230 0.79174113 13.304524 110-119
ATATG 1927140 0.7842708 5.5196147 50-59
TCATC 2140620 0.7820394 9.85521 110-119
ACATC 2268180 0.7775422 5.82277 110-119
CTATC 2125310 0.77644616 7.739119 110-119
ACAAA 2072460 0.7753857 12.788251 130-139
GGTGC 3465385 0.7713488 11.147125 60-69
GCTTG 2671450 0.7696274 16.16942 90-99
CCTCC 3030700 0.767952 10.185548 140-149
TGATG 2288080 0.76671225 12.113039 120-129
AACGC 2881100 0.7630811 15.609726 180-189
ACGCC 3304210 0.75240606 13.911984 180-189
TATGA 1832835 0.7458923 5.0112243 50-59
CAACG 2803400 0.7425016 15.890718 180-189
CCAGC 3259540 0.7422342 6.5364656 80-89
ATCTT 1625995 0.73634017 12.265734 3
GGTTA 2178275 0.7299177 8.217467 50-59
GCGGA 3486815 0.7282586 9.009166 220-229
CTGAC 2574175 0.7265959 9.243445 220-229
TCAAC 2118925 0.726377 7.338249 110-119
ATGCC 2567875 0.72481763 8.66757 50-59
ATAAA 1561405 0.72413146 7.932309 130-139
TGCCA 2565430 0.7241275 7.8304415 60-69
TATTG 1650880 0.71599644 6.5473323 240-247
GACCG 3272910 0.71376425 9.087527 210-219
CCCCC 3478850 0.71114236 13.266363 80-89
TTGGA 2118625 0.70992947 6.3880243 60-69
GGGAT 2741290 0.7097117 12.878213 100-109
TATAG 1735160 0.70614237 6.4589577 120-129
TTTAC 1552900 0.7032387 12.8533325 45-49
GAAAT 1839865 0.7025807 7.2868466 110-119
AATAC 1758865 0.70130455 11.84446 130-139
GGGCA 3310715 0.69147825 13.23684 150-159
TTTCT 1430870 0.69056094 9.52984 4
CATCC 2326535 0.6856909 6.365404 110-119
GACCA 2582080 0.6838834 5.9719944 170-179
CACGC 2998440 0.6827788 13.204929 190-199
CGCCG 3637085 0.68194044 10.811328 220-229
TCCGG 2931530 0.6813301 11.309229 70-79
GATTA 1667965 0.6787967 5.427788 50-59
GGATA 2143675 0.6740274 14.975125 35-39
CACAA 2091390 0.6727271 8.285394 130-139
AAATA 1443995 0.66968036 13.153943 130-139
GCCGC 3524160 0.6607674 11.94896 220-229
CAAAC 2040850 0.6564702 13.392727 130-139
CCCGT 2696450 0.65436417 9.992049 140-149
CAGTC 2292485 0.6470851 5.497154 210-219
TAGCT 1818730 0.6363459 13.307629 50-59
AGCAA 2052300 0.6322384 5.167939 50-59
AGGCA 2488960 0.6313443 6.458537 80-89
AGATG 2005345 0.63053286 5.9949837 120-129
TTCAT 1384675 0.6270571 5.080905 100-109
TACTT 1384115 0.6268036 11.224988 1
AAGCA 2031700 0.6258922 5.9287186 120-129
GCGAC 2864515 0.62470037 6.8863935 70-79
TCAAT 1467145 0.623433 8.389876 110-119
ACTTC 1703030 0.62217325 30.237022 4
GACGA 2447260 0.6207668 9.566211 220-229
CACCG 2655950 0.6047899 9.13519 45-49
AATAA 1303460 0.6045045 7.4151225 130-139
TTAGC 1710135 0.5983502 10.55041 45-49
CCGGC 3187085 0.59756714 9.181139 70-79
AGTAA 1552275 0.59276 5.2750745 50-59
AATGG 1883845 0.5923301 6.5815983 120-129
TTCAA 1381045 0.58684653 6.2487783 200-209
CGGTT 2030385 0.58494073 12.008187 160-169
ACCTA 1704285 0.58423656 7.482151 130-139
ACCCG 2562105 0.58342034 8.911717 140-149
CCCTA 1979305 0.58335316 11.447602 110-119
CCGTA 2049075 0.57837933 6.761029 230-239
TCCAC 1953790 0.5758332 9.712617 180-189
ACGAC 2167245 0.57401115 8.904579 220-229
CACGA 2148725 0.569106 8.353437 180-189
GGCCC 3016140 0.5655155 9.729185 70-79
ATTTC 1242435 0.56264305 6.5706334 240-247
CTTTG 1497650 0.55844194 6.976065 45-49
TAATA 1129850 0.5584254 8.101908 130-139
GTTCC 1837220 0.5526607 6.518517 140-149
GGGGA 2758530 0.5517859 8.361885 4
TGATT 1268775 0.5502752 5.816204 50-59
ATTAA 1111735 0.5494721 6.726702 110-119
GGTGA 2094855 0.5423516 6.266988 120-129
ACATT 1266590 0.5382112 6.82803 110-119
TCAGG 1984615 0.5364969 5.169071 150-159
CGGCA 2454880 0.5353662 10.590422 70-79
CTGCG 2288805 0.5319515 11.9122 25-29
CTGTT 1424040 0.53099424 5.756862 240-247
TATGT 1201445 0.52107376 7.039524 140-149
GGAGG 2583925 0.51685977 5.4701915 6
AAATC 1286610 0.51300436 7.8493137 110-119
AAGAT 1340215 0.51178163 5.672304 120-129
GCTAC 1803055 0.50893676 7.641905 50-59
CCACG 2234040 0.5087162 7.314571 180-189
GTCCT 1682690 0.506176 6.3968716 45-49
GAAGA 1711800 0.50504386 5.8615646 15-19
GGGTT 1790395 0.4939903 6.2729 100-109
ACCCC 2071945 0.49263674 8.806235 140-149
GTAAC 1488925 0.4888272 6.9270086 120-129
CGCGG 2699040 0.48466134 6.5027685 220-229
CCGGT 2077490 0.48283884 9.348526 160-169
AGTAT 1177955 0.47938174 5.3005667 110-119
ATTTA 908755 0.4786673 5.0486736 110-119
AACAT 1196065 0.47690177 6.1984906 110-119
CATAA 1194590 0.47631365 6.394023 110-119
TGGCC 2029255 0.47162834 14.131197 2
TGACG 1732220 0.46826744 8.330948 220-229
GGTTT 1307325 0.46686068 5.891906 100-109
CCATT 1269930 0.4639475 5.218109 110-119
CAATC 1340340 0.4594745 6.2693534 110-119
CCGCG 2370010 0.44436845 6.7540874 220-229
TAAAT 894960 0.44233167 7.9231877 130-139
ATTCT 971870 0.44011626 7.3966384 2
ATGCT 1246035 0.43596867 6.6260724 50-59
GCATG 1601435 0.4329126 8.807808 60-69
TGCTA 1222380 0.4276922 5.874142 50-59
TGCGC 1808185 0.42024842 8.832247 25-29
TCAAA 1042845 0.41580907 5.3501687 200-209
GCCTT 1365560 0.41077897 8.77482 1
GGTCA 1494795 0.40408486 5.1322484 160-169
ATGGC 1490105 0.40281698 6.9298844 1
CACTT 1089470 0.3980195 7.036586 3
CTAAC 1160505 0.3978263 5.5531864 110-119
GCGCT 1710600 0.39756826 7.964029 25-29
CCTAA 1138220 0.39018694 7.673494 110-119
CATGC 1355675 0.38265768 6.941289 60-69
TACCT 1046165 0.3821987 5.181965 2
ACGAG 1489485 0.37781963 7.2118278 180-189
AGCTT 1078245 0.3772616 7.0093055 1
CCGGG 2096895 0.37653536 5.0622344 80-89
GGTAA 1187655 0.37342975 7.268822 120-129
TTTGA 858170 0.37219337 5.9032083 45-49
GAGGC 1709970 0.35714555 5.3647056 35-39
TTCTT 730740 0.3526669 18.89045 1
TGCTG 1194530 0.34413633 14.857148 1
ATGTG 1018270 0.34121183 5.327767 140-149
TGGGC 1504760 0.33493966 8.731699 1
CGAGC 1455840 0.3174931 6.072392 180-189
CTCTT 775700 0.30201283 8.526465 1
GCGAG 1400590 0.2925282 5.194742 240-247
CGCTT 968405 0.29130936 5.5759654 1
TCACA 833130 0.2856007 5.3923435 5
GACTT 807170 0.28241655 6.8821545 1
AACTT 664565 0.28239316 10.617115 3
ATTGT 638410 0.27688217 5.301822 240-247
TTCCT 703085 0.2737407 6.4035697 4
CCCTT 857880 0.26945603 5.681929 3
GAGGT 1036650 0.26838556 6.892342 7
CTCGG 1154070 0.2682226 5.197205 6
TAACT 618920 0.26299727 7.460636 2
GTCTT 678485 0.25299263 11.597385 1
AATCT 587060 0.24945901 7.594471 2
TTGGC 849030 0.2446 6.840106 1
CGGGG 1390510 0.23913285 8.458699 3
TGTAA 581095 0.236483 7.208246 25-29
CCTCG 915845 0.22225378 5.6296062 5
TGCTT 541595 0.20194927 10.885742 1
TCGGT 639235 0.18415944 6.123091 7
TGCCT 611395 0.18391591 5.0582466 4
TAGGC 539880 0.14594464 6.5095267 1
TTGCT 332640 0.12403439 5.7892156 4
Produced by FastQC (version 0.10.1)