FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_1_illumina12index_2_SL248348.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_1_illumina12index_2_SL248348.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 13935984
Filtered Sequences 0
Sequence length 251
%GC 54

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TAATA 16198240 8.812241 61.742474 110-119
ATACA 15001210 7.716779 37.258804 110-119
CACAG 21604090 7.5438166 42.78758 110-119
TCCCT 25071740 6.968357 105.088005 30-34
CAGTA 18103950 6.685551 39.623276 110-119
AGTAA 14038805 6.485122 48.329918 110-119
GGAGA 22497790 6.335072 96.45851 35-39
GTAAT 15239540 5.9517465 39.95305 110-119
GGTGA 24886645 5.924649 102.97464 45-49
CCCTG 24618155 5.809932 69.059784 30-34
AATAC 10672850 5.490226 39.333214 110-119
GTAGT 19399495 5.438975 30.760086 80-89
AGACG 17166950 5.383027 103.874535 40-44
ACAGT 14364860 5.3047543 36.05324 110-119
GTGAC 18903455 5.0114007 85.58822 45-49
TAGTA 12385920 4.837276 39.61979 80-89
GAGAC 15359560 4.8162856 144.19057 40-44
CCCCA 15374820 4.779283 102.06076 60-69
CTCAG 15772290 4.65623 34.665344 140-149
ACTCC 14033215 4.6133733 119.37328 30-34
GGCCC 22382420 4.4853067 59.112537 60-69
ACCAG 12782395 4.463416 92.80722 45-49
GCACA 12537205 4.3777995 34.632507 110-119
AGTAG 13101035 4.3445797 32.201347 80-89
CGTGT 19366880 4.340724 23.165333 120-129
GTGTC 19128000 4.2871833 23.512953 120-129
TGACC 14508285 4.2830753 146.01436 45-49
CACTC 13000075 4.2737317 97.48404 25-29
CTCCC 15715320 4.130099 68.269684 30-34
CCCAG 14389810 4.0168543 87.67706 60-69
ATGCA 10820915 3.9960217 95.977394 25-29
GACCA 11275760 3.9373221 106.034775 45-49
TGAGG 16025080 3.8150167 70.33754 35-39
AGATA 8181055 3.779178 20.593603 150-159
AGGAG 13414670 3.7773888 96.96843 35-39
CCCTT 13537290 3.7625103 37.11846 20-24
ATGGT 13335860 3.7389328 25.85605 190-199
TACAC 9058285 3.7250454 27.173182 110-119
CCACT 11288910 3.7111924 27.82244 240-247
GACGG 16432415 3.6990392 110.343765 40-44
ACGGT 13903120 3.685787 97.85986 40-44
CTGAG 13802065 3.658997 106.15556 35-39
CAGGG 16051900 3.6133828 35.302967 50-59
TGTCC 14311400 3.5719576 20.507502 120-129
GAGGA 12665315 3.5663805 127.47464 35-39
TGCAC 11841710 3.495861 114.98527 25-29
TGGTG 17185625 3.4589646 18.000557 190-199
GCCCC 15469375 3.4520695 73.275635 60-69
CTTGG 15102335 3.3849056 21.424536 20-24
GGGTT 16772205 3.3757555 41.58476 50-59
CCGTG 15851235 3.359363 23.393684 120-129
AGGCT 12661895 3.3567321 26.822016 30-34
TCTTG 12673030 3.345133 18.374102 170-179
CCTGA 11328575 3.3443754 84.08162 30-34
CTCTC 12001370 3.335621 14.180142 130-139
GGATG 13924375 3.3149118 70.074196 20-24
CAGAT 8904620 3.2883592 18.441294 150-159
CTTCA 9411945 3.2722728 17.477627 200-209
TCAGG 12295760 3.2596679 21.186354 140-149
GCACT 10983905 3.2426226 88.87098 25-29
CCAGG 12917235 3.2380147 39.874153 50-59
TCGCA 10760415 3.1766448 24.075459 100-109
TCTCA 9113440 3.168491 22.076387 130-139
TGTCT 11908655 3.1433706 19.94082 180-189
CGCAC 11100730 3.098721 24.589043 100-109
CCTGG 14563425 3.0864365 28.94931 50-59
ACAGA 6962255 3.0410898 14.151439 140-149
CGGAT 11364740 3.0128498 79.331604 20-24
GGTTG 14897260 2.9983838 73.3507 15-19
ACCAC 7675600 2.9846208 20.167381 230-239
TTCCC 10523760 2.9249394 48.486 50-59
GCCCT 12393575 2.9249072 19.957142 20-24
GATGC 10943900 2.9012828 95.88212 25-29
TGGCC 13667445 2.8965504 43.334213 60-69
TCTCT 9808805 2.883173 15.489263 180-189
GGTTC 12738265 2.855044 46.730682 50-59
AGGGT 11974585 2.850734 49.62031 50-59
GATGG 11859340 2.8232982 20.82033 15-19
GTTGG 13883805 2.7944045 52.78158 15-19
GACTC 9464745 2.7941432 18.616468 190-199
TCAAA 5405395 2.7805917 57.64074 70-79
CGGCC 13833845 2.772222 12.674081 120-129
TTCTT 8909475 2.76959 20.77833 170-179
CGGTG 14518385 2.7630608 69.01801 40-44
GCCGT 12885195 2.7307677 22.100983 120-129
AGATG 8181225 2.7130668 17.67698 180-189
AGTCA 7130715 2.6332793 31.236357 70-79
TCACA 6400675 2.6321542 11.350692 140-149
CCAGT 8875945 2.6203196 35.385162 60-69
GTTCC 10455415 2.609549 52.749165 50-59
TGGAG 10951890 2.6072657 18.248186 25-29
TGTTC 9803050 2.587582 13.399527 140-149
TCCAT 7434945 2.5849245 19.44641 70-79
ACAGC 7319590 2.5558884 11.592227 120-129
GGCTG 13333765 2.5376103 13.985105 30-34
AGGGA 8929970 2.514558 8.875906 150-159
GCGGA 11145840 2.5089982 87.533676 20-24
TCCTC 9016830 2.506108 17.226643 130-139
GAGAT 7488590 2.4833744 17.242802 180-189
TACCA 5907780 2.4294608 16.657532 230-239
GGCGG 14893660 2.4068172 42.272156 20-24
GTTCT 9098585 2.4016335 17.764362 160-169
CTGGA 9024455 2.3924284 15.240722 180-189
AATAA 3697015 2.3789492 10.148508 110-119
ACTCT 6802060 2.364888 12.991525 200-209
CATTT 6425480 2.3625686 18.48843 150-159
CACGG 9403380 2.3571827 9.4486685 120-129
TTGGG 11624220 2.339616 79.409195 15-19
TCATT 6340485 2.331317 17.127007 150-159
GTGAA 7012140 2.3253736 18.820883 190-199
TTCAT 6288555 2.312223 16.618597 140-149
ACTAC 5616640 2.309735 13.583471 230-239
GATAC 6178300 2.2815652 15.463934 150-159
CAGCT 7727715 2.281344 11.859517 150-159
CTGGC 10764130 2.281249 34.147396 60-69
TGTAG 8123515 2.2775645 7.776929 210-219
TTCAC 6518185 2.2661927 19.180586 200-209
CTACT 6435720 2.2375216 8.2097845 230-239
CAGAG 7102805 2.227221 9.197748 140-149
TTGTC 8390915 2.2148395 13.591279 170-179
ATTTG 6703135 2.2132735 16.325525 150-159
CACTA 5363485 2.2056298 15.831498 240-247
TACAG 5835920 2.1551287 14.731685 160-169
GAATC 5774050 2.1322808 14.925548 190-199
TCAGC 7129895 2.1048584 13.814091 140-149
ATGTA 5378495 2.1005516 8.696985 70-79
CTCTG 8372605 2.089704 14.899093 180-189
ATATA 3834185 2.0858908 11.10523 110-119
TGAAT 5339215 2.0852108 14.412191 190-199
TGGGG 12192415 2.0837233 52.491856 15-19
GTCTC 8339800 2.0815165 12.326168 180-189
CTCGC 8817490 2.0809443 16.173546 100-109
ACACA 4274490 2.0791478 14.252305 110-119
TCTGC 8323145 2.0773594 12.303755 210-219
TTGGA 7404895 2.076087 15.898378 170-179
GTTCA 6622320 2.0675619 15.461806 140-149
GCTGT 9210470 2.0643544 10.539549 140-149
GTCCT 8247990 2.0586019 14.622595 130-139
CAGGC 8182200 2.0510647 15.712579 140-149
TAGTC 6538950 2.041533 25.750349 70-79
AATCG 5509680 2.0346525 13.573895 190-199
TTGCA 6479470 2.0229626 14.7526865 150-159
AATAT 3713330 2.0201428 13.710823 110-119
TCTCG 8021960 2.0021873 15.473958 100-109
TTTGC 7572440 1.9987973 12.510147 150-159
AGCTC 6750450 1.99284 11.877623 140-149
TACTA 4579025 1.9914407 10.909317 230-239
CCTTC 7163145 1.990901 14.379814 200-209
GAGTC 7499720 1.9882135 12.793411 210-219
TGGAA 5954650 1.9746876 9.688856 170-179
TCGGC 9303840 1.9717689 8.361187 130-139
GAACT 5330100 1.968336 10.576921 160-169
ACGGC 7832890 1.9635017 14.488158 120-129
AACCA 4036650 1.9634606 14.863881 70-79
GTCAA 5308435 1.9603354 39.781425 70-79
GATAT 5012230 1.9575082 8.096164 180-189
ATATG 4955795 1.9354677 8.348305 180-189
ATCTC 5564105 1.9344854 7.7217135 100-109
AGTAT 4945035 1.9312655 12.898678 220-229
CAGCC 6894125 1.9244653 10.46718 120-129
CCAGA 5434170 1.8975288 29.353643 60-69
ACACG 5397920 1.8848708 13.221776 120-129
CATAG 5082220 1.8767971 8.2909565 210-219
GGGGC 11492925 1.857258 47.838802 15-19
GACGT 6954370 1.8436384 9.391148 170-179
CTGTT 6977495 1.8417575 13.178319 140-149
CGTAG 6904880 1.8305185 10.019254 210-219
GCAGA 5774110 1.8105832 15.121895 150-159
GGGCG 11198675 1.8097072 59.002926 20-24
GTTGT 7626305 1.8076959 6.7350097 210-219
AGTCT 5699260 1.7793725 13.576222 210-219
ATTGT 5385980 1.7783688 8.051384 50-59
AGAAC 4009535 1.7513516 11.259043 160-169
TGCAG 6588260 1.746581 13.769347 150-159
CCTTG 6972990 1.7403768 24.263016 20-24
CACCA 4391635 1.7076666 13.321942 230-239
ATAGT 4304215 1.6809955 7.361661 220-229
CAAAA 2718120 1.6538414 22.053595 70-79
GGTCA 6233870 1.6526302 7.415368 130-139
GTCCC 6995330 1.6509112 21.884933 50-59
TCCCA 5010840 1.6472971 10.466476 240-247
AACTG 4448840 1.6428984 10.434954 160-169
TCTGG 7311315 1.6386944 12.312006 180-189
GTCAC 5550070 1.6384689 8.279216 140-149
CTCCA 4970600 1.6340684 7.318985 150-159
TGACT 5221040 1.6300668 11.200981 190-199
GAGAA 4139815 1.6238225 10.439865 160-169
AATAG 3508170 1.6205734 9.845676 240-247
GAACC 4633695 1.6180152 12.296999 70-79
TGGTT 6809845 1.6141669 17.990017 10-14
TTGGT 6762730 1.602999 25.40672 25-29
GTCTG 7145585 1.6015493 9.356303 210-219
ACTGA 4330960 1.5993667 8.621425 180-189
TCAGA 4288985 1.5838659 9.743194 130-139
TGTGC 6990770 1.5668503 8.06409 220-229
TTGTA 4742625 1.5659429 8.593701 210-219
GTGCT 6955125 1.558861 8.0283575 220-229
ATAGA 3364940 1.5544091 9.948739 240-247
TAGTT 4699825 1.551811 9.446791 220-229
ACCAT 3765480 1.5484811 12.319084 45-49
GCTCC 6557330 1.5475423 5.93385 150-159
GGCCG 8597580 1.5471758 12.994394 120-129
CCACC 4974240 1.546249 9.878443 230-239
CAAAG 3528400 1.5411932 28.609043 70-79
TTCAG 4935780 1.5410055 9.118443 150-159
GCTCT 6151395 1.5353162 10.440884 130-139
CTGTG 6817465 1.5280074 5.033249 210-219
CTGGT 6814995 1.5274537 6.7221494 160-169
CAGAC 4368320 1.5253502 17.739798 60-69
CCCAC 4906800 1.5252851 10.638885 240-247
GGACG 6768700 1.5236769 7.0010347 170-179
CCTCG 6451805 1.5226384 10.068734 130-139
TGCTC 6086920 1.519224 5.048876 150-159
GCTCA 5124830 1.512931 10.685445 140-149
AAAAA 1955560 1.4884031 8.904702 8
GCTGA 5513810 1.4617387 22.90444 30-34
AAATA 2264040 1.4568608 5.1012836 9
AATTG 3719395 1.4525962 9.75232 170-179
TATAC 3288415 1.4301478 7.137504 110-119
CGACT 4797510 1.4163009 8.28267 190-199
CATGT 4534615 1.4157573 8.556975 70-79
GCGTA 5313545 1.4086475 10.591777 210-219
TGATA 3603615 1.4073787 7.6040826 180-189
ACGGA 4474030 1.4029181 9.836485 200-209
CACTG 4734670 1.3977495 7.100778 230-239
ACCGT 4713915 1.3916223 20.95544 45-49
TCGAC 4697675 1.3868283 8.952526 190-199
CTCTT 4657475 1.3690053 7.8043733 200-209
GAATT 3491455 1.3635751 10.784627 170-179
TGGAC 5108940 1.3544056 9.878854 170-179
GGGAG 6680150 1.3503678 6.3981066 150-159
GAGGG 6662430 1.3467855 7.957289 200-209
CCATC 4065810 1.3366218 10.482416 240-247
GTGGA 5610935 1.3357693 23.059488 25-29
CCTCA 4037055 1.3271687 7.1617637 130-139
TATGG 4716860 1.3224512 6.0641584 180-189
GTCCA 4457840 1.3160249 18.238588 70-79
GTGTT 5544500 1.3142366 7.863989 160-169
CTCGA 4432155 1.3084424 8.004251 190-199
ACTTC 3756160 1.305913 6.6003423 220-229
AGCCG 5205560 1.3048985 9.303729 120-129
GGAGT 5478625 1.3042709 7.3935895 210-219
GGCTC 6113580 1.2956551 8.77905 130-139
CCATG 4364240 1.288393 8.658991 70-79
CTGAT 4122795 1.2871826 7.163352 180-189
TCCAC 3900925 1.2824162 9.317511 240-247
GGAGG 6331965 1.2799833 17.376287 30-34
ATCGG 4817630 1.277178 8.166723 190-199
TTGGC 5688805 1.2750392 8.794897 60-69
CTTGA 4073830 1.271895 8.101416 200-209
CTGCT 5088425 1.2700113 5.5770955 90-99
GGAAT 3816845 1.2657464 9.086113 170-179
ATAAC 2438300 1.2542871 7.500284 80-89
ACGTG 4713305 1.2495209 8.976547 170-179
GTGGT 6204345 1.2487534 11.904363 50-59
AGATC 3374930 1.2463174 6.646246 130-139
AAGTA 2697820 1.2462382 15.8774395 70-79
ACTAT 2855990 1.242084 6.2288003 230-239
ATAAA 1918795 1.2347031 5.905121 7
TGGTC 5479560 1.2281408 13.438122 50-59
ACTCG 4124225 1.2175366 8.222844 190-199
AAAGT 2600835 1.2014365 21.03158 70-79
TCACG 4055980 1.1973895 9.858457 200-209
ATACC 2893620 1.1899456 8.38787 70-79
ACTGG 4481505 1.1880696 6.9624434 160-169
TTGAG 4237050 1.1879284 7.791886 200-209
TGCCC 5014290 1.1833819 7.4838443 60-69
GATCT 3779615 1.180038 5.2379766 130-139
CCATA 2868840 1.1797552 11.516257 70-79
TTCCA 3370310 1.1717637 7.4948306 230-239
GCATA 3167395 1.1696774 7.7004356 210-219
AGCTT 3697035 1.1542555 6.1147423 150-159
AGTTC 3633805 1.1345145 5.607548 160-169
ACTGT 3632660 1.134157 5.3568354 230-239
CGGCT 5340710 1.1318603 8.284714 130-139
CTCGG 5327825 1.1291294 8.72715 130-139
TATCA 2596220 1.1291088 7.1704273 70-79
AAAAG 2054280 1.1224401 15.088383 70-79
TATGT 3381445 1.1165018 8.006257 220-229
AGAGC 3544660 1.1114963 6.942351 140-149
CAATA 2152155 1.1070911 6.551842 100-109
AGGTT 3946820 1.1065575 23.370665 10-14
TCTAC 3165105 1.1004194 7.116606 180-189
CTACC 3339400 1.0978167 8.930438 230-239
GTCTA 3474190 1.0846808 7.2827964 180-189
ATGGG 4554010 1.0841521 32.80763 15-19
GAAGA 2760135 1.0826496 18.719694 35-39
CTGCG 5083855 1.0774246 7.249926 210-219
TCTGT 4076975 1.0761454 6.0952077 210-219
AGAGA 2723775 1.0683876 14.019983 35-39
ATCCA 2590055 1.0651102 14.71807 240-247
TACTG 3400595 1.0617037 6.3190284 180-189
GCTTC 4226015 1.0547639 5.3859587 150-159
GAGCT 3972230 1.0530581 6.1603208 140-149
ATATC 2396235 1.0421343 7.6120634 70-79
TGCGT 4612975 1.0339121 8.39241 210-219
ATACG 2794625 1.0320183 5.5893273 120-129
AGAAA 1879020 1.0266796 5.008345 8
CTGCC 4316810 1.0187753 5.5427055 200-209
CTGAA 2742070 1.0126104 15.315754 35-39
TATAA 1850470 1.0067011 6.615114 240-247
AACTT 2309100 1.0042391 5.6039534 200-209
CGGAG 4460190 1.0040168 6.9407754 210-219
TCCCC 3809210 1.0010877 5.3472047 80-89
GCGGT 5233315 0.99597627 5.5744505 130-139
CAGCG 3964290 0.9937444 6.668913 160-169
TAGAT 2534880 0.98998815 7.5679784 240-247
GGGGT 5789995 0.9895292 7.5373683 10-14
CATGG 3728030 0.9883196 5.1207557 190-199
AAAAT 1532630 0.9862143 5.4158216 8
ACTAA 1895350 0.97498786 13.079911 240-247
CGAAC 2792145 0.97497416 12.883496 70-79
ATGTG 3473115 0.97374636 5.0223765 220-229
ACCCA 2500770 0.97241265 12.979522 240-247
GAGTT 3415970 0.9577248 5.395136 160-169
CGTGG 5019710 0.955324 8.253932 50-59
AGCTG 3581825 0.94955987 5.0389943 90-99
CCATT 2724225 0.9471377 6.054467 50-59
ACAAT 1826025 0.93932635 7.530029 100-109
TGAAG 2830145 0.9385357 10.202566 35-39
CTTCT 3180865 0.9349747 5.1187882 210-219
CCCAA 2385625 0.9276392 9.489539 240-247
TGTAA 2370350 0.92573154 6.5793023 230-239
AAGAG 2340100 0.91789305 13.926595 35-39
ACGTC 3090270 0.91229683 12.478034 70-79
TAGGC 3410310 0.9040903 5.48201 160-169
GGTGG 5220990 0.8922843 22.80774 25-29
TGCAT 2851165 0.8901655 5.895751 210-219
TCGAA 2397715 0.8854447 16.49706 70-79
GAGGC 3931655 0.88504004 14.746947 30-34
CATCA 2130090 0.8759585 5.6045303 230-239
AGCGT 3289580 0.8720842 6.827917 160-169
TGAAC 2338835 0.863701 6.8177295 190-199
ATCAA 1674715 0.8614909 13.632376 70-79
CCAAT 2085705 0.85770607 10.297056 240-247
GTATG 3025170 0.8481574 5.6410503 220-229
GGGCC 4649280 0.8366601 17.345695 15-19
CCAGC 2987090 0.8338333 5.632098 80-89
AACCG 2382920 0.8320792 6.030441 190-199
CGTCC 3520930 0.83094615 10.164473 70-79
CGGTC 3910545 0.82876444 5.331389 130-139
CTTCC 2977240 0.82748425 5.3205433 230-239
TTCTG 3123410 0.8244454 5.1094975 210-219
GACCG 3224185 0.8082193 19.30039 45-49
GGTCC 3770520 0.7990888 13.609477 50-59
TAACC 1925080 0.79165214 6.0625677 80-89
TGGGC 4134870 0.786926 18.804815 15-19
CTAAT 1782810 0.7753528 9.970037 240-247
AAAGG 1928760 0.7565468 9.036649 10-14
TGAGA 2271685 0.7533387 6.018764 240-247
AAAGA 1376345 0.7520225 5.833059 8
CATAC 1825945 0.7508848 9.9596815 70-79
AGCCA 2117920 0.73954517 5.8982177 80-89
AATCC 1771730 0.7285899 9.082292 240-247
GTAGC 2689700 0.713053 5.593303 80-89
ATGAT 1822505 0.7117727 6.5225534 240-247
GTCGA 2667560 0.70718354 11.58211 70-79
CCTTT 2396710 0.70448244 5.0915217 50-59
AAAGC 1591345 0.6950942 10.223072 70-79
AAGGT 2093150 0.6941327 9.990908 10-14
CATTG 2152490 0.67203134 5.5664043 50-59
CGATG 2498330 0.6623199 14.41131 15-19
TAGGT 2349485 0.6587177 5.429264 10-14
TATGA 1662640 0.64933795 5.688601 240-247
CCGTA 2168110 0.64006054 5.3608427 80-89
AGGAT 1927680 0.63925934 6.202289 10-14
GAGGT 2649015 0.6306387 6.8723187 10-14
CAATC 1530985 0.6295881 8.789077 240-247
GGAAG 2206145 0.6212204 6.2190185 30-34
AGAAG 1577905 0.61892563 5.6955047 9
AGACC 1766295 0.6167631 5.223563 240-247
ATAGG 1833205 0.60792947 5.3175464 10-14
AATGG 1761335 0.58409584 6.658502 10-14
TAAAA 879730 0.56608725 5.8323016 8
CATCC 1715915 0.5641014 6.944506 240-247
GGGTC 2913410 0.55446446 5.516978 70-79
AAGAA 998685 0.5456725 5.4511166 9
AAATG 1177595 0.5439814 5.359407 9
AAGCA 1216345 0.5312955 6.865888 70-79
GAAGG 1847255 0.52016187 5.550039 10-14
AAGGG 1826925 0.5144373 6.355123 10-14
GAAAA 923435 0.50455654 5.866128 8
GGTCG 2518160 0.47924256 5.599847 70-79
TTGCC 1916945 0.478447 5.3078 60-69
ACGAT 1290685 0.476633 6.6229095 10-14
ATAAG 1026970 0.47440127 5.789574 9
TACCG 1492980 0.44075134 5.301636 70-79
CGGTT 1941960 0.43525404 7.9844275 10-14
TAAGG 1305740 0.43301094 5.9690223 10-14
GAAGC 1365230 0.42809412 5.088127 30-34
AAGCT 1112175 0.41071168 8.494137 30-34
TAAAG 876895 0.40507522 5.7108436 9
GAAAG 952225 0.37350565 5.192177 9
Produced by FastQC (version 0.10.1)