FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s2_2_illumina12index_15_SL248344.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s2_2_illumina12index_15_SL248344.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 9841072
Filtered Sequences 0
Sequence length 251
%GC 54

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 41335 0.42002537934891643 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 34646 0.3520551419601442 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 34205 0.34757392284092625 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 32998 0.33530899885703513 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 32607 0.33133585446788727 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29678 0.30157283678038327 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 28255 0.2871130299625894 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 27218 0.2765755600609365 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26738 0.27169804265226394 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 26613 0.2704278558270887 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 26453 0.26880201669086456 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26417 0.26843620288521414 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25270 0.256780968577407 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25241 0.2564862852339664 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24268 0.24659915098680305 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24113 0.2450241193235859 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23933 0.2431950502953337 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23930 0.2431645658115295 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23681 0.2406343536557806 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23573 0.23953691223882928 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23323 0.23699653858847897 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22947 0.23317581661835216 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22473 0.228359268177288 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22447 0.22809506931765156 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21105 0.21445834356257124 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20778 0.21113553482791306 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20662 0.20995680145415052 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20385 0.2071420674495624 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19725 0.20043548101263764 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19690 0.2000798287015886 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19523 0.19838285910315462 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18870 0.19174740312843966 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18839 0.19143239679579624 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18748 0.19050770078706872 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18525 0.18824168749095627 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18134 0.18426854310180843 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18115 0.18407547470438182 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 17864 0.1815249395594301 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 17817 0.18104734931316427 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 17692 0.1797771624879891 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17516 0.1779887394381425 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17044 0.17319251398628116 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 16862 0.17134312196882615 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16657 0.16926001557553894 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 16652 0.1692092081025319 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 16145 0.16405733033962155 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16078 0.16337651020132765 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 15547 0.15798075656798363 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 15450 0.15699509159164773 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 15336 0.155836681207088 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 15279 0.15525747601480813 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15114 0.15358082940557696 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14394 0.14626455329256813 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 13953 0.14178333417335023 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13930 0.14154961979751798 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 13881 0.14105170656204935 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 13850 0.1407367002294059 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 13744 0.13965958180165738 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12997 0.13206894533441071 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12721 0.12926437282442402 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12455 0.1265614152604513 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12357 0.125565588789514 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 11743 0.11932643110425369 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11721 0.11910287822302285 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10515 0.10684811573373307 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10243 0.10408418920215196 No Hit
ATGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 9903 0.10062928103767557 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 19638190 7.544291 66.19812 35-39
TATTA 9799265 7.1263485 123.79771 240-247
TGGAT 14840510 7.019692 77.90185 35-39
TCACC 15235095 6.397116 73.98668 160-169
TCTCC 13596755 6.153138 87.673584 20-24
CTGTG 14297235 5.9195867 87.50216 25-29
TCCCT 12878240 5.8279786 73.243095 15-19
AGTGG 15282020 5.6154776 39.381557 100-109
CTGAG 14507805 5.5733805 50.169514 15-19
TGTAT 8964900 5.4585595 58.81154 240-247
TTACT 8172970 5.2026367 109.898445 240-247
ATTAC 8265095 4.8816786 102.97679 240-247
TGGGT 12322955 4.880262 45.400772 100-109
CCTGA 12065825 4.8460183 51.893764 15-19
CTCTG 10928690 4.730624 73.636635 35-39
TTCAC 8986435 4.6459956 73.89328 40-44
CTCCA 10998345 4.6181326 41.431732 80-89
ACACG 12210950 4.5504584 48.879147 230-239
GACAC 12173225 4.5364 46.302547 230-239
TGAGA 10000190 4.3888946 92.96361 15-19
TCTGG 10398780 4.3054814 134.7597 35-39
ATTCA 7253600 4.284251 110.17699 40-44
TACTG 8550140 4.2281804 77.62339 240-247
AAGGG 12389685 4.224194 38.567913 90-99
CCCTG 11928685 4.1936355 86.087234 10-14
CAGCC 12670605 4.1330733 55.684116 30-34
AGCCT 10273625 4.126214 50.922382 30-34
CCAGG 13092090 4.0848274 53.041573 80-89
TGGAG 11091400 4.075607 38.267673 90-99
GAAGG 11934630 4.0690455 30.63951 150-159
GAGAC 11283785 4.0220685 84.87418 8
ACCAT 8335895 3.9987228 65.68238 160-169
TCCAG 9884070 3.9697564 40.657383 80-89
TCAGT 7959185 3.935944 92.80153 7
GGGTC 12236875 3.9359224 126.12437 8
CACCA 10037170 3.9104617 56.320232 160-169
GTATT 6364540 3.875249 67.888 240-247
ACTAC 8047100 3.8601878 34.55481 130-139
CTGGG 11782955 3.7899215 133.8464 3
CACCT 8991080 3.7752953 44.212532 20-24
TACTA 6388930 3.7735443 27.907011 130-139
GACTC 9385815 3.7696414 52.15153 20-24
ACTGT 7489315 3.7035859 66.89519 240-247
GATTC 7477995 3.6979883 58.75776 40-44
AGACT 8013740 3.6769962 68.28723 15-19
TGAAG 8315000 3.6492968 39.108795 150-159
GGACA 10019720 3.5714967 28.069515 220-229
GCTGG 11051800 3.5547497 33.13649 90-99
CCTGT 8183215 3.5422103 62.594494 25-29
CAAGA 8302715 3.534726 42.936623 180-189
ACTGG 9080235 3.4883018 43.50439 60-69
ACTCT 6700685 3.4642606 68.44914 20-24
GAGTG 9326315 3.427015 38.179142 100-109
TCCTG 7741755 3.3511186 64.04427 20-24
CTCCT 7292285 3.3000846 94.44161 20-24
TATCT 5175500 3.2945485 48.41737 200-209
GGTCC 9741140 3.2756455 144.3194 9
GGATT 6807710 3.2201068 55.963657 40-44
AGACA 7547500 3.2132072 36.954346 170-179
CTGCA 7960545 3.1972077 44.753975 25-29
AGCTG 8252255 3.1702216 35.98832 60-69
CATCT 6127305 3.1678226 32.864395 170-179
CCATC 7450360 3.1283572 34.698677 45-49
GTCTC 7203040 3.1179287 33.161556 30-34
GTCCC 8783550 3.0879352 76.00775 10-14
CCTCA 7121415 2.9902353 79.44466 5
GGAGT 8040160 2.9544086 36.63821 90-99
GGCTG 9121910 2.9340112 34.2483 90-99
GTGTA 6148165 2.9081366 35.24076 230-239
AGGGA 8484660 2.8927977 39.548077 80-89
ATCTC 5579035 2.8843665 48.164406 170-179
AAGAA 5906105 2.8725443 35.942883 180-189
TGTGC 6801530 2.8160858 57.691692 25-29
AGTAG 6362400 2.7923377 27.886374 50-59
GAGCT 7226840 2.776294 29.119095 60-69
GGAAG 8095525 2.760124 29.64776 80-89
GTGAA 6287850 2.7596188 38.198456 150-159
ATCTG 5555580 2.7473228 36.49821 200-209
AAAAA 4727880 2.746458 19.76786 90-99
CAGGG 9019330 2.6917076 36.03975 80-89
GACAA 6311780 2.6871228 24.387579 180-189
ACCTT 5134975 2.654787 69.89743 40-44
GAACA 6235385 2.6545987 34.26279 210-219
CTATG 5337400 2.6394293 27.38924 50-59
GGGAA 7707010 2.6276624 33.293068 80-89
ATGAA 4907925 2.5726802 26.93541 210-219
CACGG 8234240 2.5691428 44.25507 230-239
CCCTC 6953885 2.5558577 27.727516 20-24
CGCCA 7786325 2.5398512 39.801758 70-79
GAGTC 6578460 2.5272093 27.249496 150-159
TGCAA 5466265 2.5081222 35.312504 200-209
AGGGC 8347380 2.4911728 32.385826 150-159
AGAAC 5847345 2.489398 29.708534 190-199
CAGTG 6397215 2.4575813 72.1063 8
CCAAG 6583365 2.4533167 38.077457 180-189
ACGGC 7825080 2.4414816 48.763416 230-239
TATAT 3340355 2.4292164 24.012135 240-247
AGGCT 6305080 2.4221869 19.98009 80-89
ATCAG 5269425 2.4178047 26.16394 45-49
CTCTC 5314090 2.4048629 98.86429 20-24
CTACA 5001835 2.3993764 18.961952 190-199
TCTGT 4494615 2.3954942 24.099794 210-219
CACTG 5923725 2.379156 19.29986 30-34
CCGCC 8307630 2.372045 34.44322 70-79
GCAGC 7586650 2.36709 34.364204 30-34
GTATC 4782285 2.3649163 22.82784 200-209
CTGTC 5461905 2.364256 47.9835 30-34
GTGGA 6432655 2.3637207 26.039015 100-109
TGAAC 5096615 2.338513 37.336445 210-219
GTGGT 5851185 2.3172457 30.811766 120-129
ACAGC 6183225 2.304203 26.711447 210-219
CTACT 4448470 2.2998633 22.30891 50-59
GGGCT 7129845 2.2932749 42.259815 90-99
TATGC 4607280 2.2783735 20.773771 50-59
CTTCA 4402580 2.276138 50.041378 45-49
GGCCG 8687070 2.269327 24.96332 230-239
CAAAT 4122025 2.2589653 34.372143 200-209
TGGTG 5701385 2.2579205 33.51516 35-39
TCCAA 4691275 2.250401 41.886673 180-189
GCCTC 6392700 2.2474105 70.44119 4
CAGGC 7170305 2.2371871 32.91979 70-79
TATCA 3749620 2.2146678 16.526768 170-179
GCTCC 6286840 2.2101946 34.340416 80-89
GCCTG 6562160 2.206652 30.87309 210-219
GGGGG 9134070 2.1830611 95.61276 5
ACAAC 4903685 2.1825755 19.719906 180-189
AATGA 4151950 2.1764064 25.48259 210-219
AAATG 4142645 2.1715288 32.55596 200-209
CCTTC 4721855 2.13685 55.379604 45-49
CAGTA 4653760 2.135315 21.01746 45-49
CCTCT 4677435 2.116748 49.19342 35-39
GTGGG 6857590 2.1097763 30.069294 100-109
TACAT 3549230 2.09631 17.593866 190-199
TGCAG 5443850 2.0913327 43.271687 30-34
GCTGT 5025450 2.080723 19.420927 230-239
GCTAT 4186665 2.0703728 33.14967 50-59
GTAGT 4367235 2.0657406 24.445518 120-129
CTCAC 4895410 2.0555506 55.327076 20-24
GCAGA 5763975 2.0545502 24.041805 140-149
TCTAT 3213415 2.0455515 19.562607 110-119
AGAGT 4609780 2.0231454 29.275408 150-159
AACAG 4716810 2.0080938 32.919907 210-219
TGCAC 4954440 1.9898604 26.061523 25-29
CAGAG 5568835 1.984993 32.33677 170-179
GGCTC 5871890 1.9745358 19.882532 40-44
TGTGA 4169225 1.9720801 18.451118 210-219
TCCGC 5586835 1.9641017 42.226177 70-79
TCTCT 3502840 1.951794 32.77011 35-39
AACTA 3558550 1.9501679 35.119164 130-139
AGCAC 5189230 1.9337869 20.151924 130-139
GGTGG 6280315 1.9321744 18.03731 40-44
TGAGC 5014410 1.9263572 17.283298 200-209
ATGGA 4385110 1.9245421 11.863172 120-129
CCGTG 5720525 1.923636 19.793766 230-239
CTGTA 3887280 1.922322 23.981417 190-199
TGGTA 4051885 1.9165775 30.233593 120-129
GGTCT 4625585 1.915164 35.082554 15-19
TTCAG 3868060 1.9128174 46.78185 45-49
CAGAC 5132215 1.91254 26.264217 140-149
ATATC 3227765 1.9064404 13.116681 160-169
CATAT 3204980 1.8929827 13.903439 160-169
CCAGA 5065115 1.8875349 34.05539 170-179
TGGGG 6113745 1.8809283 124.03214 4
GCCAG 6023870 1.8794911 35.38541 70-79
ACCCT 4444995 1.8664242 43.831562 10-14
AGAGA 4564960 1.8589237 27.765512 170-179
AGGAC 5195840 1.8520403 20.594368 220-229
GGATC 4798405 1.8433758 17.814964 70-79
CCTGC 5242815 1.8431584 48.989136 20-24
TCCAT 3558150 1.839567 26.510355 45-49
ACTAT 3112415 1.8383102 16.436878 130-139
CTATT 2882260 1.8347493 19.227024 110-119
GCACA 4904610 1.8277221 17.89705 130-139
GTCAC 4545930 1.8257898 37.16418 160-169
CAGTT 3674445 1.8170716 24.252028 190-199
TGTCT 3405650 1.8151088 48.96751 30-34
GATGG 4936770 1.8140482 20.215363 100-109
ACCAG 4857210 1.8100584 13.714626 190-199
TTCCA 3489165 1.8039017 22.719025 180-189
GAGGA 5263860 1.7946839 26.749357 220-229
TGGGA 4879800 1.793114 16.11639 100-109
GCCGA 5744185 1.7922275 19.360287 220-229
GCTGA 4636930 1.7813429 26.240864 200-209
TCAGC 4423105 1.7764595 11.531565 45-49
CACAG 4746035 1.7686285 15.404558 140-149
ATACT 2982605 1.7616396 26.055496 130-139
ACCTG 4352705 1.7481846 30.817673 25-29
TAGTG 3689890 1.7453505 25.628393 120-129
CAAGG 4868580 1.7353894 16.660355 80-89
TAGTA 3040590 1.7177821 16.443596 120-129
TTCTG 3222040 1.7172502 41.54057 30-34
GCAAA 4032645 1.7168235 31.70768 200-209
CCTGG 5099650 1.7148551 34.25228 2
AGTCA 3719605 1.7066908 28.06524 160-169
AGTTC 3448570 1.705373 21.42654 190-199
GTGCA 4411760 1.6948406 50.155056 25-29
TACAA 3077395 1.6864839 17.095636 130-139
CTGAA 3658800 1.6787912 22.408346 200-209
CTCAG 4178920 1.6783869 75.63462 6
TGACC 4178320 1.678146 14.180007 210-219
CATGA 3649520 1.674533 22.768 60-69
GCCGT 4970235 1.6713368 28.962784 230-239
ATGCA 3610055 1.6564251 16.364157 60-69
AACAC 3715250 1.6536163 22.350655 190-199
AGCTA 3581735 1.643431 25.162256 50-59
GGAGC 5484040 1.6366441 12.773503 200-209
TCTGC 3766225 1.630259 33.68918 200-209
GGATG 4434175 1.6293664 21.596117 100-109
TCTCA 3126725 1.6165198 26.05609 100-109
ATTGG 3409635 1.6127875 19.981548 100-109
AGGGG 5629665 1.6070342 32.60018 90-99
GGGCC 6146945 1.6057689 27.508509 2
GGGGC 6347600 1.5860695 28.7554 90-99
CGATT 3196295 1.580619 34.82945 160-169
CAGCT 3917035 1.5732055 12.73336 50-59
CTCCC 4276555 1.5718216 14.086612 190-199
TCTGA 3161810 1.5635654 20.846891 220-229
ACAAT 2811720 1.5408877 22.807617 180-189
GTTCT 2883175 1.5366453 22.799576 190-199
GCACT 3819855 1.534175 25.087807 25-29
TTCTC 2716365 1.5135676 21.754942 190-199
CGTCC 4302640 1.5126314 18.739504 180-189
GTCCG 4492475 1.5106809 34.700916 70-79
CGGCC 5503910 1.5031632 31.595459 230-239
CCATG 3728750 1.4975845 15.069999 160-169
AGTGA 3385630 1.4858892 81.96971 9
AGTTA 2622805 1.4817545 15.395405 50-59
GGCTT 3573790 1.4796818 20.679852 25-29
CTGGT 3552020 1.4706682 30.958036 35-39
TTAGT 2411585 1.4683689 24.938023 110-119
ACTCC 3495345 1.4676723 17.19347 140-149
GAGCA 4116485 1.4673079 7.3890424 200-209
AAGAG 3586855 1.4606237 18.077492 150-159
CATCA 3035310 1.4560359 30.46649 45-49
TACGC 3606305 1.4484066 24.565548 140-149
CTTTA 2269020 1.4443817 41.629303 45-49
CGTGT 3478505 1.4402303 23.412008 230-239
GTTAC 2852800 1.4107552 15.294689 50-59
GGGGT 4568320 1.4054695 118.41438 7
GTGAC 3651965 1.4029545 14.845788 210-219
CTACG 3428950 1.3771752 16.163322 130-139
GGCAG 4609140 1.3755411 13.165633 150-159
CTCAA 2861580 1.3726977 18.083817 150-159
GTCCA 3406060 1.3679819 28.066696 180-189
GAGAG 4006145 1.365873 25.50417 220-229
GGCCT 4044825 1.3601501 67.12627 3
TAGCA 2962580 1.35934 11.411425 130-139
CAACC 3482120 1.356627 15.005436 140-149
AACCC 3474535 1.3536719 19.74303 140-149
CAACT 2820140 1.352819 17.11281 130-139
TGTGT 2614575 1.3328849 15.113096 240-247
GAACT 2904230 1.3325667 14.375152 190-199
AGCAG 3728705 1.3290848 12.292865 50-59
AGAGC 3728165 1.3288921 24.386068 220-229
ATCAT 2230700 1.317536 15.51498 110-119
TGGAA 3001685 1.3173829 19.075949 120-129
AAGCT 2871015 1.3173264 15.978418 200-209
TCACT 2542880 1.3146714 13.223989 190-199
CGACA 3507215 1.3069774 15.673294 70-79
ATCTA 2212280 1.3066565 19.998888 110-119
ACAGT 2844640 1.3052247 11.985966 120-129
CAATT 2205470 1.3026342 23.811785 180-189
ATACA 2371785 1.2997931 20.7136 35-39
TGTCC 3001265 1.2991363 54.146217 15-19
TTATT 1655925 1.2978861 20.293673 240-247
GTGGC 4032855 1.2971452 14.269281 40-44
CCTTT 2325560 1.2958097 48.342762 45-49
CACGT 3224085 1.2948949 16.308826 180-189
CCATA 2695730 1.2931396 14.360534 160-169
TCCGT 2980020 1.2899401 13.861182 150-159
GTGCG 4008210 1.2892183 16.88206 240-247
TCAAG 2792545 1.2813216 22.413357 150-159
ACAGA 3000960 1.2776027 19.387125 140-149
AATTC 2143385 1.2659644 23.757668 180-189
ATATT 1739850 1.2652763 25.63554 240-247
TTACA 2140225 1.264098 9.378208 120-129
ACATG 2753925 1.2636014 13.922096 190-199
CCTAC 3007130 1.2626741 16.936083 190-199
ATTAT 1736120 1.2625637 9.689695 50-59
AGATC 2743875 1.25899 12.089284 210-219
AGACC 3360195 1.2521898 98.160675 9
AACCA 2811315 1.2512848 16.877546 190-199
ACATA 2280615 1.2498299 20.08626 130-139
CCAGT 3109455 1.2488558 18.508825 190-199
GATCT 2521870 1.247105 9.244033 220-229
TCGGA 3239450 1.244481 91.65553 5
TGCGA 3224560 1.2387607 13.8998375 240-247
CGGCT 3671595 1.2346443 21.45696 230-239
ACACA 2742600 1.2207005 7.556622 130-139
ACGTC 3014535 1.2107328 18.011143 180-189
ATCCG 3008085 1.2081424 23.301325 70-79
CTTCT 2164720 1.2061892 22.616507 30-34
GATTG 2547250 1.2048718 23.014374 100-109
AGTCG 3135995 1.2047372 15.64962 150-159
TGGAC 3068895 1.1789598 15.265765 80-89
CGCTG 3490930 1.173892 12.441796 190-199
TGAGT 2479800 1.1729673 20.06215 90-99
TACCA 2431770 1.1665182 10.413493 45-49
GTCGA 3035230 1.166027 12.224232 150-159
CTTCG 2691970 1.1652539 104.17652 3
GGGTG 3754735 1.1551654 14.206476 60-69
ATTAG 2036510 1.1505269 24.628925 110-119
ACGCA 3086065 1.1500342 20.783653 140-149
ATTCC 2221165 1.1483445 22.269335 180-189
GATCC 2857405 1.1476246 23.149979 70-79
AAGGC 3182145 1.1342651 27.453707 25-29
GAAGT 2579320 1.1320149 12.963041 140-149
GGACT 2941495 1.1300173 12.885717 90-99
AGGCC 3604855 1.1247411 13.964975 2
GACCC 3440285 1.1221999 43.170403 10-14
TCGAG 2905575 1.1162181 11.917582 150-159
AGCTC 2763760 1.1100137 12.0464945 210-219
TTGGG 2795490 1.1070982 14.949059 100-109
GAACC 2968865 1.1063591 10.161227 180-189
GTATA 1952765 1.1032151 10.062256 240-247
TTCGG 2657260 1.1002043 99.48287 4
CGCAG 3522025 1.0988973 16.407816 140-149
GCCCC 3848225 1.0987686 12.386319 80-89
GCAAG 3075765 1.0963465 16.457376 25-29
AGGTC 2847515 1.0939136 17.55719 10-14
CCGAT 2713925 1.0899984 23.506477 160-169
ACAAG 2540150 1.0814215 16.545124 80-89
GCTTC 2496890 1.080811 27.392637 2
GTAGA 2460660 1.0799372 16.439732 170-179
CCCCT 2925150 1.0751209 9.575047 140-149
CACAT 2238135 1.0736318 13.513238 130-139
GCACC 3274360 1.0680761 10.942728 130-139
CTTGA 2147270 1.0618593 21.442917 90-99
CTCCG 3015375 1.060082 9.989572 140-149
TTGAG 2240465 1.0597597 19.846935 90-99
TAGAC 2290975 1.0511829 16.830845 170-179
GGAGA 3073375 1.04785 80.9544 7
ATGGG 2850180 1.0473171 19.391865 100-109
TACAG 2281495 1.0468333 8.986231 120-129
GAAGC 2922540 1.0417298 12.4911175 200-209
ATCAA 1899515 1.0409783 12.106326 110-119
TGGCT 2509080 1.0388523 30.87253 40-44
CAGCA 2777350 1.0349903 12.18373 45-49
ATGAG 2347185 1.0301353 19.47581 60-69
ACACC 2642690 1.0295868 12.684889 40-44
CGTGA 2675845 1.027964 20.577948 150-159
GGGAG 3589745 1.0247223 14.548996 5
CTATA 1733615 1.0239388 12.625625 50-59
GAGCC 3277945 1.0227427 17.989779 220-229
ATGGT 2148490 1.0162548 9.258064 35-39
GCTCT 2335730 1.0110509 16.894516 210-219
CGAGG 3387515 1.0109621 14.68063 220-229
ACCGC 3091025 1.0082734 10.969437 220-229
ACTCA 2093455 1.0042288 12.160833 190-199
GACAG 2815510 1.0035794 15.018322 70-79
TGAGG 2724580 1.0011646 11.830025 220-229
AGCCG 3200085 0.9984498 18.986296 220-229
GTAGC 2598285 0.99816823 11.006598 50-59
TAGTT 1625995 0.99003786 14.315294 50-59
TTATA 1359905 0.9889678 13.042106 110-119
TTTCA 1550380 0.98691964 7.256461 100-109
AACTC 2051785 0.9842397 16.198788 190-199
CGTAT 1985270 0.98174775 7.4671817 240-247
AAACT 1785675 0.9785913 16.205576 130-139
ATGAT 1722495 0.97312397 9.068018 120-129
GCCAA 2595650 0.96727914 14.6184635 180-189
ACCAA 2172360 0.9668932 16.45161 130-139
ATGAC 2106510 0.9665437 10.392298 160-169
CCGTC 2743825 0.96461606 10.246876 140-149
TTTAG 1579155 0.9615179 29.354792 45-49
CCGAG 3069640 0.9577499 17.851065 220-229
CGAGT 2480685 0.95299053 14.6687155 160-169
GCAGT 2472300 0.9497693 6.047743 50-59
CCAAC 2430760 0.9470193 13.7482395 130-139
GGTAT 1995820 0.9440405 9.313903 110-119
AAGGT 2149265 0.9432719 19.929323 15-19
GCCAT 2347580 0.9428626 7.74356 60-69
CGGAC 3009500 0.9389859 10.754106 220-229
GGGAC 3133240 0.93507695 9.908529 90-99
GATAC 2035930 0.93415904 22.833773 35-39
CATAC 1944050 0.9325593 13.821509 130-139
AGCCC 2825195 0.9215613 11.361923 80-89
TTTAT 1174665 0.9206826 10.687161 240-247
AACTG 2005115 0.92002 11.49044 60-69
TATGG 1941150 0.91818106 11.954929 35-39
CATTA 1552060 0.9167055 11.959203 110-119
AATCA 1663125 0.9114311 13.400883 110-119
CAGAA 2136090 0.90940046 17.32858 140-149
ACAGG 2547585 0.9080783 14.567197 70-79
TACAC 1882540 0.903053 20.744814 35-39
CCCAG 2767990 0.90290135 14.51176 80-89
CCCCA 2638735 0.8998759 16.763723 80-89
TAACA 1638720 0.89805657 8.250274 120-129
TCATA 1519565 0.8975127 8.629093 110-119
GAGAT 2035365 0.89328337 11.615742 210-219
TATTT 1133850 0.8886925 10.974633 240-247
GTCTG 2137505 0.8850065 15.988842 3
GTTAT 1451940 0.884059 10.701021 110-119
ACGCT 2194725 0.88147116 15.16739 190-199
GACTG 2293555 0.8811019 12.513219 90-99
AGTCT 1778760 0.87962526 15.912935 8
TCCTT 1576220 0.87827504 10.6989 1
TGGTT 1717665 0.875649 8.958948 50-59
AAGTT 1547840 0.8744526 16.68439 140-149
CGGAG 2929390 0.8742404 71.08278 6
GATGA 1968075 0.863751 6.6188846 60-69
AGAAG 2092380 0.8520501 15.0415945 140-149
ATAGT 1500365 0.8476316 15.143131 120-129
GTTTC 1573980 0.8388839 5.0734253 100-109
GGTAG 2278615 0.8372919 11.708776 120-129
ATCCA 1743980 0.8365859 9.515646 180-189
TCTTC 1498420 0.8349246 45.33092 2
GGTTC 2012595 0.8332891 11.410677 160-169
GCCTA 2068245 0.83067274 16.239826 190-199
TTACC 1601480 0.8279667 9.83181 45-49
GTCTA 1671320 0.82649446 9.7396555 30-34
GATCA 1796215 0.8241691 9.006283 110-119
ATATA 1220790 0.8237438 10.254171 130-139
CTATC 1592445 0.8232956 8.114783 110-119
CATGG 2141905 0.8228434 8.414722 200-209
TGGCA 2127140 0.81717116 5.8312144 100-109
TCATC 1577535 0.8155871 10.355649 110-119
ACAAA 1597330 0.8122155 12.205793 130-139
ATATG 1431555 0.80875736 5.358573 50-59
CCTCC 2194075 0.8064188 10.559656 140-149
TTATG 1318735 0.802953 11.344896 50-59
GCTTG 1937400 0.80215555 16.507439 90-99
GGTGC 2492325 0.8016424 11.355895 60-69
ACATC 1668730 0.80048853 5.570899 110-119
TGATG 1683905 0.79650193 12.10949 120-129
CGCCG 2811400 0.7678164 10.848651 220-229
TATAC 1297260 0.7662109 8.665691 130-139
GTTCA 1537570 0.76035285 13.759928 160-169
ATAAA 1195605 0.74854267 7.690185 130-139
TGACT 1510470 0.7469515 5.0230756 2
CTGAC 1847765 0.7421211 9.233849 220-229
CCAGC 2273585 0.7416295 6.212212 80-89
CCCCC 2482575 0.7410701 13.474124 80-89
CATCC 1753040 0.73608994 6.5952106 110-119
ATTAA 1084290 0.73163867 6.385862 110-119
ATCTT 1145965 0.72948265 11.934504 3
GCGGA 2441905 0.7287565 8.976266 220-229
GGGAT 1973400 0.72513866 12.8120575 100-109
TCAAC 1511260 0.72495025 7.1846547 110-119
TTGGA 1529115 0.72328484 6.389313 60-69
AAATA 1152630 0.72163695 12.267865 130-139
ACGCC 2201810 0.71821684 12.969813 180-189
TATTG 1178160 0.71735954 6.4605403 240-247
GACCG 2289705 0.71440464 9.17136 210-219
TTTCT 1040015 0.71351904 9.676606 4
GGTTA 1507940 0.713269 7.891713 50-59
GATTA 1262250 0.71310854 5.588718 50-59
AACGC 1912960 0.712872 14.256917 180-189
TTTAC 1117325 0.7112514 13.228137 45-49
ATGCC 1766495 0.70948046 8.070035 50-59
TGCCA 1764500 0.70867926 7.437507 60-69
TCCGG 2099920 0.7061384 11.651283 70-79
AATAC 1277210 0.6999407 11.095102 130-139
CAACG 1865065 0.6950238 14.549118 180-189
CACAA 1547620 0.6888284 8.156166 130-139
GACCA 1846700 0.68817997 5.900496 170-179
GGGCA 2300770 0.6866365 13.168913 150-159
GAAAT 1295235 0.678948 6.9718738 110-119
TATAG 1200625 0.6782934 6.1148834 120-129
GGATA 1543290 0.6773209 14.989967 35-39
CAGTC 1684180 0.6764201 5.544998 210-219
AATAA 1071010 0.6705364 7.1902733 130-139
CCGTA 1669130 0.6703755 6.273844 230-239
CAAAC 1504155 0.6694826 13.160936 130-139
AGGCA 1855345 0.6613318 6.619231 80-89
CACGC 2026460 0.66101885 12.389569 190-199
GCCGC 2416235 0.65989363 11.911872 220-229
CCCGT 1862665 0.6548365 9.992697 140-149
AGATG 1477980 0.64865756 5.7552414 120-129
TCCAC 1521940 0.63905257 10.098025 180-189
ACTTC 1235335 0.6386694 30.666378 4
GAAGA 1564150 0.63694656 6.2665315 15-19
GACGA 1784540 0.6360935 9.413508 220-229
GCGAC 2037735 0.63578814 7.0394096 70-79
TACTT 989245 0.62972 11.383737 1
TCAAT 1056580 0.6240562 8.119771 110-119
CACCG 1889890 0.61647046 8.73276 45-49
AAGCA 1434050 0.61052 5.744268 120-129
CCGGC 2231745 0.6095079 9.521465 70-79
AGTAA 1159875 0.6079937 5.131296 50-59
GGCCC 2202790 0.6016001 10.168778 70-79
TAGCT 1212460 0.5995808 12.176159 50-59
CCCTA 1426710 0.59906614 11.509574 110-119
CACGA 1604055 0.59775734 8.592776 180-189
GGTGA 1623930 0.59672356 6.434581 120-129
AATGG 1352040 0.59338486 6.464524 120-129
TGATT 973490 0.5927398 6.038041 50-59
CTTTG 1111345 0.59231335 7.2054205 45-49
ACGAC 1553330 0.5788545 8.735505 220-229
ATTTC 907150 0.57746106 6.4651756 240-247
GGGGA 2022460 0.57732785 9.368467 4
AAGAT 1093940 0.5734313 5.7136045 15-19
TAATA 847735 0.5720201 7.8916316 130-139
ACCTA 1187960 0.5698635 6.9559827 130-139
ACCCG 1745985 0.56952953 8.585711 140-149
GTTCC 1306615 0.56558514 6.612838 140-149
GTCCT 1295715 0.560867 6.2687793 45-49
CGGTT 1353925 0.56057525 11.299474 160-169
TTAGC 1130480 0.5590404 9.462366 45-49
TTCAA 938685 0.554423 5.280862 200-209
TCAGG 1435055 0.55129683 5.148543 150-159
CGGCA 1757465 0.5483419 10.770216 70-79
CTGCG 1627965 0.54743445 11.851812 25-29
CTGTT 1024300 0.5459211 5.7099814 240-247
GGAGG 1899665 0.542275 5.2972217 6
CCTAT 1035670 0.5354424 5.001615 110-119
TATGT 879245 0.5353558 6.5740504 140-149
CCACG 1632750 0.532593 7.64067 180-189
AAATC 951770 0.521592 7.558155 110-119
GCTAC 1298545 0.5215369 7.6771746 50-59
ACCCC 1518615 0.5178864 8.93537 140-149
ACATT 873190 0.51573914 6.076645 110-119
GGGTT 1253320 0.49635252 5.841714 100-109
AACAT 893300 0.4895491 5.6686673 110-119
GTAAC 1058775 0.48580462 6.630222 120-129
CGCGG 1858245 0.4854301 6.4355245 220-229
TGGCC 1435060 0.48256648 14.653797 2
TGACG 1250335 0.48033404 8.287824 220-229
TATCC 924650 0.47804496 5.365335 110-119
GGGTA 1299395 0.47747108 5.0500736 100-109
TAAAT 695345 0.469193 7.7254796 130-139
CAATC 977210 0.4687669 6.1470456 110-119
CCGGT 1383075 0.46508548 8.858575 160-169
CATAA 844400 0.4627508 5.9010634 110-119
ATGCT 931135 0.46046114 6.965048 50-59
GGTTT 891535 0.4544959 5.3812985 100-109
TGCTA 906965 0.44850868 6.1765885 50-59
CCGCG 1638005 0.44735262 6.6685266 220-229
ATTCT 697860 0.4442341 7.4103384 2
TGCGC 1303865 0.43844965 8.751745 25-29
TTGGT 848840 0.43273038 5.129767 120-129
GCATG 1124085 0.4318333 8.42799 60-69
GCCTT 997610 0.43182835 8.812448 1
GGTCA 1093415 0.42005098 5.3563876 160-169
CACTT 804265 0.4158058 7.30174 3
ATGGC 1072945 0.41218713 6.9867625 1
GCGCT 1216595 0.40910342 7.91406 25-29
ACGAG 1131390 0.40328026 7.461662 180-189
CATGC 999760 0.40153536 7.1161737 60-69
TACCT 775615 0.4009937 5.006234 2
AGCTT 800625 0.39592186 7.045069 1
CTAAC 824440 0.39548326 5.207416 110-119
CCGGG 1501425 0.39221784 5.293188 80-89
TTTGA 643105 0.39157453 6.02752 45-49
CCTAA 796870 0.38225794 7.1893344 110-119
CTCGG 1127410 0.37911323 5.035285 6
TTCTT 547845 0.3758579 19.613194 1
GAGGC 1253300 0.37403196 5.704901 35-39
GGTAA 847790 0.37207904 6.9412274 120-129
TGCTG 839245 0.3474786 13.639311 1
TGGGC 1066500 0.34303376 9.273013 1
CGAGC 1068400 0.33334857 6.3946967 180-189
CTCTT 567900 0.31643575 8.943052 1
GCTTT 587580 0.31316245 5.168342 45-49
TCGGT 749925 0.3104968 5.8930664 7
TCACA 645285 0.30954272 5.0884113 5
AACTT 507535 0.2997694 10.68527 3
GCGAG 1001880 0.29899877 5.2112293 240-247
CGCTT 688455 0.29800662 5.737368 1
GACTT 601270 0.29733762 6.6622453 3
ATTGT 481735 0.2933194 5.2845974 240-247
GAGGT 776780 0.28543285 6.665373 7
CCCTT 623790 0.2822928 5.561804 3
TTCCT 506080 0.28198946 6.3069363 4
TGTAA 475480 0.26862255 7.8465514 25-29
TAACT 453600 0.26791334 7.3987985 2
AATCT 448795 0.26507533 7.1463594 2
TTGGC 630935 0.2612305 7.2446685 1
GTCTT 486180 0.2591193 11.227062 1
CGGGG 1009710 0.25229537 9.249456 3
CCTCG 692485 0.24344927 5.643469 5
TGCTT 428825 0.2285508 11.325816 1
TGCCT 455655 0.19723615 5.248169 4
TCGCA 486135 0.19524725 7.4341264 7
TAGGC 417120 0.1602426 6.749014 1
TTGCT 243790 0.12993272 5.5434713 4
Produced by FastQC (version 0.10.1)