FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_11_SL248340.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_11_SL248340.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 12502379
Filtered Sequences 0
Sequence length 251
%GC 54

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 58438 0.4674150415692886 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 44020 0.35209298966220753 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 43972 0.351709062731181 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 42598 0.34071915433054784 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 41155 0.32917735096656403 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 38712 0.3096370698728618 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 38700 0.30954108814010517 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 38680 0.3093811185855108 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 37967 0.3036782039642215 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 37698 0.30152661345492726 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 35458 0.2836100233403579 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 34337 0.27464372980534346 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 32531 0.2601984790254719 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 31955 0.25559135585315407 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 31852 0.254767512646993 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 31802 0.2543675887605071 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 31221 0.2497204731995407 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30747 0.2459291947556541 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 30084 0.24062620402085078 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 29848 0.2387385632766372 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 28686 0.2294443321547043 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 28468 0.22770066400962571 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 28100 0.2247572242050893 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27699 0.22154983463547215 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27560 0.22043804623104132 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 27340 0.21867838113050325 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26500 0.2119596598375397 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26208 0.20962410434046194 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26172 0.20933615914219209 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25695 0.20552088526511633 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 25560 0.20444109077160433 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25369 0.20291338152522814 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 24646 0.19713048212664167 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 24369 0.19491490379550963 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23900 0.19116361774027166 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 23643 0.189108008963734 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23449 0.18755630428416864 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 22633 0.18102954645671834 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 22593 0.1807096073475296 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 21812 0.17446279624061947 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21353 0.1707914949626787 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21304 0.1703995695539225 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21189 0.16947974461500487 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 20552 0.16438471430117418 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20540 0.16428873256841758 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20417 0.1633049198076622 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 20202 0.16158524709577274 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 20029 0.16020151044853143 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19950 0.15956963070788366 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 19514 0.15608229441772642 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 18673 0.1493555746470332 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 18626 0.1489796461937364 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18211 0.14566027793590325 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18138 0.14507638906163378 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 17242 0.13790975301580605 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17003 0.13599811683840332 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 16916 0.1353022492759178 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 16509 0.1320468688399224 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16412 0.1312710165001397 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 16129 0.12900744730262936 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15704 0.125608094267499 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 15568 0.1245203012962573 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14926 0.11938527859377804 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 14818 0.11852144299896844 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14783 0.1182414962784283 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13715 0.10969912206308895 No Hit
CAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTG 13569 0.10853134431455007 No Hit
AAGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 13339 0.1066916944367148 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12713 0.10168464737791104 No Hit
ATGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 12572 0.10055686201802073 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 25153795 7.6045604 65.32626 35-39
TATTA 12477210 7.265528 124.97341 240-247
TGGAT 19141735 7.1852684 77.84562 35-39
TCACC 19536730 6.3893385 73.99345 160-169
TCTCC 17219890 6.0542703 85.056114 20-24
CTGTG 18098040 5.8820624 85.11792 25-29
TCCCT 16507320 5.8037415 72.33474 15-19
AGTGG 19601755 5.697691 39.242744 100-109
CTGAG 18471795 5.584441 48.55192 15-19
TGTAT 11333585 5.493975 58.58882 240-247
TTACT 10563025 5.3256717 111.8189 240-247
ATTAC 10641725 4.990806 103.98544 240-247
TGGGT 15571125 4.865768 44.84543 100-109
CCTGA 15268475 4.801014 49.999454 15-19
CTCTG 13908300 4.7015257 72.434265 35-39
GACAC 15666430 4.582261 45.562664 230-239
TTCAC 11256945 4.5710464 72.96889 40-44
ACACG 15618835 4.5683413 48.22363 230-239
CTCCA 13827685 4.5222387 39.96543 80-89
TGAGA 12495280 4.36295 91.501274 15-19
TACTG 11039520 4.310013 78.99296 240-247
TCTGG 13213655 4.2945833 134.11618 35-39
AAGGG 15703245 4.245862 38.67675 90-99
ATTCA 8973555 4.20846 108.34867 40-44
CCCTG 15306360 4.167213 84.252335 10-14
TGGAG 14262355 4.145674 38.376614 90-99
GAAGG 15183020 4.1052027 30.500952 90-99
AGCCT 13046025 4.102187 49.98837 30-34
CAGCC 16169085 4.094791 54.002747 30-34
CCAGG 16661690 4.05694 52.17343 80-89
ACCAT 10705290 4.04358 67.16604 160-169
TCAGT 10321645 4.0297427 99.13184 7
GAGAC 14092945 3.9631903 88.659 8
CACCA 13002115 3.9554014 57.062813 160-169
ACTAC 10438860 3.9429448 35.791996 130-139
GTATT 8098115 3.925576 68.11422 240-247
TCCAG 12376135 3.8915477 39.48296 80-89
TACTA 8236750 3.8629096 28.074207 130-139
GGGTC 15281060 3.845863 123.49351 8
ACTGT 9689450 3.7829232 68.29597 240-247
CACCT 11550250 3.777421 44.459442 20-24
CTGGG 14908325 3.752055 130.98961 3
TGAAG 10653090 3.7197163 38.6677 150-159
GACTC 11689885 3.6757631 50.214764 20-24
GATTC 9283575 3.6244626 57.03551 40-44
AGACT 9941835 3.610501 67.06096 15-19
GGACA 12818040 3.604664 28.169666 220-229
CAAGA 10606325 3.5829303 43.606133 180-189
GCTGG 14049345 3.5358713 32.47901 2
ACTGG 11681275 3.5315137 44.23314 60-69
CCTGT 10316540 3.4873765 60.180557 25-29
GAGTG 11730655 3.4097786 39.232826 90-99
ACTCT 8290595 3.3665168 66.12677 20-24
TCCTG 9838450 3.3257642 63.895985 20-24
TATCT 6564020 3.3094513 47.789753 200-209
AGCTG 10777975 3.2584252 36.777603 60-69
CTCCT 9217935 3.2408962 92.48253 20-24
AGACA 9581980 3.2368958 37.75341 170-179
GGATT 8618385 3.235099 54.669155 40-44
CTGCA 10253460 3.2240942 46.419334 25-29
GGTCC 12070195 3.159521 139.89468 9
GTCTC 9320590 3.150708 34.13352 30-34
CATCT 7691315 3.1231706 31.90215 170-179
CCATC 9499650 3.106788 35.82158 45-49
CCTCA 9352840 3.058775 83.50886 5
GTCCC 11121615 3.0279 72.8226 10-14
GGAGT 10186135 2.9608293 36.65741 90-99
AGGGA 10739810 2.9038424 39.685062 80-89
AAGAA 7433425 2.9001873 37.288418 180-189
GGCTG 11474975 2.8879662 33.544468 90-99
AGTAG 8208695 2.8662124 28.733545 50-59
GAGCT 9401855 2.8423932 29.462795 60-69
GTGTA 7518280 2.8221507 36.61739 230-239
TGTGC 8609715 2.7982523 56.33378 25-29
GTGAA 7932040 2.7696133 39.91375 10-14
ATCTC 6811155 2.7657683 46.933163 170-179
ATCTG 7074630 2.7620535 35.963993 200-209
CAGGG 11698795 2.7387605 35.814644 80-89
GGAAG 10123945 2.7373242 29.911154 80-89
GACAA 8013000 2.7068772 23.863657 180-189
ACCTT 6558175 2.6630425 71.85291 40-44
CTATG 6811080 2.6591592 27.667267 50-59
GAACA 7752715 2.6189504 33.76118 210-219
GGGAA 9672490 2.6152594 33.494144 80-89
GAGTC 8587945 2.5963302 28.572847 150-159
CCCTC 9095245 2.57546 27.402182 20-24
CACGG 10507410 2.55844 43.631275 230-239
ATGAA 6094655 2.5563107 26.56425 210-219
CAGTG 8360455 2.5275548 76.7344 8
ATCAG 6957775 2.5268025 27.549831 45-49
TGCAA 6940570 2.5205543 34.682827 200-209
AGGGC 10736915 2.5135784 32.66174 150-159
AGAAC 7406785 2.5020914 29.546871 190-199
CGCCA 9799555 2.481719 38.65587 70-79
CTACA 6565660 2.4799676 20.010077 190-199
TATAT 4222440 2.4587433 23.723616 240-247
CCAAG 8365285 2.446756 38.459595 180-189
AGGCT 8064920 2.4382076 20.910349 25-29
GTGGA 8378725 2.4354649 26.948988 100-109
ACGGC 9990315 2.4325333 48.251293 230-239
TCTGT 5724495 2.4026637 25.07285 210-219
CTGTC 7098755 2.3996446 49.233765 30-34
CACTG 7606990 2.3919394 19.881796 30-34
GCAGC 9724105 2.367714 33.206123 30-34
CTACT 5802890 2.3563478 23.153933 50-59
CTCTC 6584970 2.3151824 95.11631 20-24
CCGCC 10536270 2.3103063 33.126602 70-79
TATGC 5906520 2.306004 21.363344 50-59
ACAGC 7876730 2.3038585 26.017855 210-219
GTATC 5900480 2.303646 22.278864 200-209
TGAAC 6325280 2.2971041 36.818184 210-219
GGGCT 9108730 2.2924411 41.817776 90-99
CTTCA 5631310 2.2866752 49.448006 45-49
TGGTG 7282175 2.275582 34.616974 35-39
GTGGT 7270395 2.2719011 30.962759 120-129
TCCAA 6012425 2.2710009 42.474503 180-189
GGCCG 11084645 2.2468343 25.474548 230-239
CAAAT 5125445 2.2359536 34.24127 200-209
CAGGC 9171015 2.2330425 33.16077 70-79
GCCTC 8176560 2.226098 73.75832 4
TATCA 4716820 2.2121162 17.031181 170-179
ACAAC 6257510 2.1985772 19.480957 180-189
CAGTA 6018490 2.1856897 21.509462 45-49
GCTCC 8005855 2.1796234 33.013466 80-89
TACAT 4630090 2.1714413 18.82821 190-199
GCCTG 8268160 2.164292 30.693153 210-219
AATGA 5130965 2.1521053 25.01289 210-219
AAATG 5111210 2.1438196 32.587 200-209
CCTTC 6065310 2.1324773 54.10593 45-49
GGGGG 11365315 2.129597 93.78421 5
GTAGT 5622805 2.1106427 24.635283 120-129
GTGGG 8670770 2.0981247 29.398685 100-109
GCTAT 5372655 2.0975742 33.95726 50-59
GCAGA 7383335 2.076327 23.767595 140-149
TCTAT 4117790 2.0761096 20.653265 110-119
CCTCT 5835535 2.051692 47.354366 35-39
TGCAG 6781380 2.0501645 42.05435 30-34
CTCAC 6236985 2.0397584 55.83501 20-24
TGCAC 6473415 2.0354984 27.279114 25-29
AACTA 4657910 2.0319934 37.419662 130-139
GCTGT 6245110 2.0297298 18.88293 230-239
AGAGT 5797940 2.0244539 30.99091 150-159
AACAG 5894230 1.9911342 32.430542 210-219
AGCAC 6801060 1.9892364 20.449877 130-139
TGTGA 5282855 1.9830351 19.210152 210-219
ATGGA 5677995 1.9825733 12.381903 100-109
CAGAG 7013880 1.9724295 31.494612 170-179
TCTCT 4512615 1.9699298 33.294933 35-39
TGAGC 6514560 1.9694989 18.160784 200-209
TGGTA 5229885 1.9631516 31.100872 120-129
ATATC 4173670 1.9573873 13.7234745 160-169
GGCTC 7452500 1.9507831 20.774954 40-44
GGTCT 5953470 1.9349432 36.822018 15-19
CCGTG 7390180 1.93447 20.592905 230-239
AAAAA 4095915 1.9196332 13.715928 90-99
CATAT 4081750 1.9142784 14.495319 160-169
TTCAG 4899235 1.9127432 46.350357 45-49
GGATC 6320970 1.9109722 18.295225 70-79
GCACA 6521535 1.9074789 18.529655 130-139
CAGAC 6520045 1.9070427 25.739264 140-149
GGTGG 7873530 1.9052111 18.420637 40-44
TCCGC 6981320 1.9006897 40.928696 70-79
CTGTA 4857495 1.8964471 23.88513 190-199
TCCAT 4663800 1.8938037 27.297457 45-49
GTCAC 6013020 1.8907318 38.62026 160-169
ACCCT 5741875 1.8778363 44.211647 10-14
CCTGC 6886355 1.8748351 50.715904 20-24
ACCAG 6409355 1.8746674 13.926158 190-199
ACTAT 3993035 1.8726724 17.211058 130-139
TGGGA 6412845 1.8640376 17.128841 100-109
GATGG 6395225 1.8589159 21.309923 100-109
TGGGG 7679330 1.8582193 121.91491 4
CTATT 3672895 1.8518022 19.80468 110-119
TGTCT 4401180 1.8472469 50.596268 30-34
GCTGA 6099990 1.844165 27.625628 200-209
GCCAG 7568320 1.8428036 34.462578 70-79
CACAG 6273855 1.8350352 16.213743 140-149
CCAGA 6270340 1.8340067 32.948204 170-179
CAGTT 4691800 1.8317571 24.880941 190-199
TCAGC 5803475 1.8248428 11.842724 45-49
AGGAC 6475120 1.8209206 20.548828 220-229
AGAGA 5573185 1.8101319 27.360197 170-179
TTCCA 4444765 1.8048614 22.382565 180-189
CAAGG 6397730 1.799157 17.551117 80-89
AGTCA 4897355 1.7785354 29.344238 160-169
GAGGA 6560940 1.7739547 26.607172 220-229
ACCTG 5592890 1.7586265 30.988558 25-29
ATACT 3738950 1.7535102 26.117613 130-139
TACAA 4013365 1.7508134 17.828192 130-139
TAGTG 4657100 1.7481443 25.758566 120-129
AGTTC 4472440 1.7461152 22.147596 190-199
GCCGA 7170360 1.7459047 18.922035 220-229
TTCTG 4157365 1.7449138 43.904713 30-34
TAGTA 3836515 1.7299314 16.581316 120-129
TGACC 5491905 1.7268728 14.549477 210-219
CTGAA 4719860 1.7140758 23.366322 200-209
CCTGG 6545380 1.7133334 33.706726 2
CTCAG 5424670 1.7057314 80.02438 6
GGAGC 7252245 1.6977955 13.518552 200-209
GCAAA 5022490 1.696651 31.219091 200-209
CATGA 4665745 1.6944236 22.566814 60-69
AGCTA 4631445 1.6819669 25.489407 50-59
GGATG 5785875 1.6817946 22.698877 100-109
ATGCA 4621725 1.6784369 16.177107 60-69
ATTGG 4390215 1.6479632 20.885923 100-109
GCCGT 6292570 1.6471573 29.780611 230-239
GTGCA 5443260 1.6456205 49.11402 25-29
AACAC 4651560 1.6343263 21.778517 190-199
CAGCT 5147625 1.6186177 12.960498 50-59
TCTGA 4094480 1.5985533 21.747286 220-229
TCTCA 3922055 1.5926074 25.778532 100-109
CTCCC 5622910 1.5922143 13.96103 190-199
AGGGG 7063295 1.5898404 32.171326 90-99
TCTGC 4684815 1.5836427 32.753464 200-209
CGATT 4014895 1.5674821 34.60026 160-169
GGGGC 8040475 1.5669831 28.036058 90-99
GGGCC 7707090 1.5622113 28.46121 2
GTTCT 3707555 1.5561212 23.454967 190-199
GCACT 4941960 1.5539484 26.208183 25-29
GGCTT 4778110 1.5529385 22.131905 25-29
CGTCC 5636520 1.5345633 19.20389 180-189
TTCTC 3511960 1.5331056 22.26809 190-199
ACAAT 3513545 1.5327691 22.405094 180-189
GAGCA 5441745 1.5303168 7.883646 200-209
AGTGA 4371065 1.5262353 88.3946 9
CCATG 4828470 1.5182625 15.879148 160-169
AGTTA 3364500 1.517094 16.294989 50-59
CGGCC 7173255 1.5122803 31.709639 230-239
CATCA 3991410 1.5076271 32.036537 45-49
CTGGT 4584845 1.4901252 32.255302 35-39
GTTAC 3759940 1.4679433 16.013744 50-59
GTCCG 5589015 1.4629931 33.489635 70-79
CTTTA 2880030 1.4520551 41.420948 45-49
TTAGT 2994290 1.4514873 24.911867 110-119
GTGAC 4721785 1.4275024 15.337582 210-219
CGTGT 4391650 1.4273348 24.596394 230-239
TACGC 4532850 1.4253079 24.173931 140-149
GGCAG 6084200 1.4243487 13.961682 150-159
ACTCC 4354520 1.4241126 16.781374 140-149
CTCAA 3721760 1.4057755 18.659544 150-159
GTCCA 4467645 1.4048047 29.03511 180-189
AAGAG 4312730 1.4007449 19.019735 150-159
GGGGT 5698695 1.3789518 116.465096 7
AAGCT 3789295 1.3761296 16.962002 200-209
CAACC 4522100 1.3756777 15.179743 140-149
AACCC 4512845 1.3728621 20.238075 140-149
CAACT 3626815 1.3699131 17.876942 130-139
GGCCT 5224535 1.3675859 70.72813 3
AGCAG 4852220 1.3645316 12.43431 50-59
TAGCA 3754230 1.3633953 11.493725 130-139
CGACA 4660440 1.3631287 16.578672 70-79
CTACG 4297740 1.35138 16.358774 130-139
ATCTA 2871175 1.3465371 21.107637 110-119
GTGCG 5321855 1.3393787 17.573072 240-247
ACAGT 3676415 1.3351358 12.649252 120-129
GAGAG 4934875 1.3342973 25.090967 220-229
CACGT 4243125 1.3342068 16.699232 180-189
TTACA 2839695 1.3317735 9.808155 120-129
ACAGA 3903300 1.3185767 20.439373 140-149
ATACA 3022130 1.3183913 21.869415 35-39
TGTCC 3895700 1.3168923 54.920277 15-19
GAACT 3626030 1.3168379 14.071931 190-199
CCATA 3465420 1.3089514 14.891471 160-169
GTGGC 5196960 1.3079457 14.622907 40-44
TCAAG 3600210 1.307461 23.43376 150-159
ACATG 3593190 1.3049116 14.800894 190-199
TGCGA 4301220 1.300356 14.594846 240-247
TGGAA 3722820 1.299889 18.708422 120-129
TTATT 2068960 1.2951776 20.25282 240-247
TGTGT 3190955 1.2876855 14.7729645 240-247
CAATT 2745180 1.2874475 23.552082 180-189
TCACT 3161255 1.2836735 12.945977 190-199
CCAGT 4071335 1.2801893 19.011395 190-199
AACCA 3640325 1.2790287 17.325628 190-199
CCTTT 2926175 1.2773879 46.931984 45-49
AGAGC 4540920 1.2769885 23.976254 220-229
AGACC 4339795 1.2693431 101.93385 9
ATCAT 2695945 1.264357 15.596512 110-119
TCCGT 3737720 1.2634891 13.488689 150-159
CCTAC 3862965 1.2633532 17.525517 190-199
ATTAT 2156185 1.255555 9.290718 50-59
AATTC 2673920 1.2540275 23.532562 180-189
CTTCT 2870725 1.2531818 23.69701 30-34
GATTG 3324270 1.2478374 24.063404 100-109
ACGTC 3966370 1.247184 18.532438 180-189
ATATT 2140295 1.2463022 25.285896 240-247
ACACA 3544660 1.2454169 7.678515 130-139
ACATA 2849500 1.2430823 20.367167 130-139
ATCCG 3911180 1.2298301 23.99905 70-79
AGTCG 4047625 1.2236885 16.287216 150-159
GATCT 3126540 1.2206534 9.560893 220-229
TCGGA 4035430 1.2200017 95.435555 5
TGGAC 4023305 1.2163361 16.311783 80-89
TGAGT 3236330 1.2148272 21.454247 90-99
CGGCT 4608530 1.2063394 20.69127 230-239
TACCA 3164380 1.1952431 11.086693 45-49
GTCGA 3907805 1.1814177 12.64445 150-159
CTTCG 3483495 1.1775516 107.715675 3
AGATC 3237155 1.175613 12.844531 210-219
GATCC 3736355 1.1748581 23.833544 70-79
GGGTG 4834310 1.1697906 14.9631195 60-69
AAGGC 4157060 1.1690402 29.493725 25-29
AGGCC 4750820 1.156773 14.903664 2
GAAGT 3309945 1.1557262 13.882685 140-149
ATTAG 2540285 1.1454455 24.55013 110-119
ACGCA 3916175 1.1454391 20.522633 140-149
AGCTC 3642020 1.1451955 12.412355 210-219
CGCTG 4366640 1.1430215 12.127109 190-199
GGACT 3776525 1.1417289 13.372391 90-99
TCGAG 3765360 1.1383533 12.359402 150-159
ACAAG 3358225 1.1344446 17.822073 80-89
TTGGG 3625080 1.1327888 15.549525 100-109
GACCC 4468770 1.1317077 43.4362 10-14
ATTCC 2780865 1.1292106 21.816116 180-189
AGGTC 3719325 1.1244361 18.437212 10-14
GCAAG 3987995 1.1214961 17.263174 25-29
TTCGG 3450550 1.1214668 103.56094 4
GAACC 3831320 1.1206197 10.56559 180-189
CCCCT 3957075 1.1205074 9.978828 70-79
GCCCC 5104185 1.1192036 12.450284 80-89
GTATA 2480555 1.1185125 9.893182 240-247
CTTGA 2848305 1.1120259 22.708769 90-99
GCTTC 3281815 1.1093763 27.98778 2
TTGAG 2951400 1.1078725 21.170776 90-99
CGCAG 4468780 1.0880994 16.116045 140-149
GCACC 4291080 1.0867082 11.127612 130-139
ATGGG 3728605 1.0838028 20.337944 100-109
CACAT 2859715 1.0801656 13.486004 130-139
TACAG 2971365 1.0790882 9.36367 120-129
GAAGC 3836685 1.0789449 13.226114 200-209
TAGAC 2962205 1.0757616 17.529268 170-179
GGAGA 3939735 1.0652303 85.09531 7
CCGAT 3381915 1.0634083 22.887505 160-169
CAGCA 3629355 1.0615473 12.770258 45-49
TGGCT 3259200 1.0592759 32.22462 40-44
ACACC 3474150 1.0568787 13.036379 40-44
ATCAA 2421315 1.0562884 12.348168 110-119
CTATA 2238680 1.0499066 13.348414 50-59
GTAGA 2996110 1.0461452 17.183338 170-179
GGGAG 4641345 1.0446963 14.242182 5
GCTCT 3068545 1.0372831 17.501059 210-219
ATGGT 2757950 1.0352567 9.322172 120-129
CTCCG 3799645 1.0344673 9.743326 140-149
GACAG 3676265 1.033832 15.978875 70-79
TAGTT 2121375 1.0283402 15.226659 50-59
AAACT 2340985 1.0212448 17.464321 130-139
ACCGC 4031905 1.0210725 11.1873665 220-229
GAGCC 4183430 1.0186197 17.569832 220-229
ATGAG 2910880 1.0163857 19.397167 60-69
TGAGG 3465880 1.0074359 11.905685 220-229
CGTGA 3331865 1.0072981 20.214846 150-159
GTAGC 3300735 0.99788684 10.988721 50-59
AAGGT 2852675 0.9960624 21.066975 15-19
ATGAC 2729350 0.9911974 11.122055 160-169
ACTCA 2621915 0.99034435 11.919799 190-199
TTTCA 1957895 0.98713267 7.0250764 100-109
CGAGT 3256925 0.9846421 15.103119 160-169
AGCCG 4035650 0.9826368 18.549307 220-229
ACCAA 2795960 0.982361 17.186298 130-139
CGAGG 4192740 0.98154646 14.306198 220-229
GGTAT 2610680 0.9799757 9.287355 110-119
TTATA 1681540 0.97916734 12.961088 110-119
GGGAC 4114905 0.9633248 10.266996 90-99
CCGTC 3516590 0.9574044 10.143758 140-149
AACTC 2530570 0.9558417 15.715826 190-199
GATAC 2627815 0.9543237 23.752235 35-39
GCCAA 3261110 0.9538394 14.473145 180-189
CAGAA 2814835 0.9508815 18.44144 140-149
ACAGG 3379280 0.95031446 15.520754 70-79
ATGAT 2106095 0.9496639 8.730997 120-129
CCAAC 3118865 0.94879663 14.259669 130-139
GCAGT 3119120 0.9429805 6.15311 50-59
TTTAG 1943195 0.9419673 29.313984 45-49
CGGAC 3862875 0.9405681 10.828662 220-229
AGCCC 3703380 0.937874 11.514452 80-89
CCGAG 3846330 0.93653953 17.49376 220-229
TACAC 2477595 0.9358321 22.211845 35-39
TAACA 2123135 0.92620856 8.773548 120-129
CATAC 2442360 0.9225232 13.812207 130-139
GCCAT 2931825 0.9218821 7.4520645 60-69
TTTAT 1471505 0.92116827 10.603408 240-247
GAGAT 2633140 0.9194078 12.421357 210-219
AAGTT 2038915 0.9193716 17.9248 140-149
TATGG 2445595 0.91800755 11.951186 35-39
AACTG 2520440 0.91532916 11.496788 60-69
CGTAT 2336600 0.91224766 7.2470794 240-247
CCCAG 3601045 0.911958 14.830715 80-89
TGGTT 2237500 0.9029261 9.063663 50-59
AATCA 2069120 0.90264475 13.439649 110-119
CCCCA 3423220 0.9016703 16.939205 80-89
GACTG 2977450 0.9001504 13.047011 90-99
AGAAG 2741435 0.8903991 16.199198 140-149
CGGAG 3781945 0.88537675 73.86754 6
TCCTT 2024920 0.8839554 10.830779 1
TCATA 1884180 0.88365155 8.635211 110-119
TATTT 1392240 0.87154806 10.518518 240-247
GATCA 2396200 0.8702098 9.490489 110-119
GTTAT 1792530 0.86893207 10.4745 110-119
ATCCA 2285990 0.86345947 10.121469 180-189
AGTCT 2197790 0.85805386 15.49923 8
TTACC 2111710 0.85749054 9.868538 45-49
CATGG 2828670 0.8551709 8.804881 200-209
GCCTA 2711255 0.85252607 16.848454 190-199
CATTA 1813430 0.85047096 11.998146 110-119
CTATC 2087375 0.8476089 8.442912 110-119
GTCTG 2605995 0.8469771 14.412125 3
ATAGT 1873070 0.84459007 15.313729 120-129
ACGCT 2680920 0.84298766 14.808651 190-199
TCTTC 1928040 0.84166354 47.627617 2
GCTTG 2583500 0.83966607 17.429008 90-99
GGTAG 2888675 0.83965826 11.859743 120-129
GATGA 2395010 0.8362605 6.389711 60-69
GGTGC 3295220 0.82932496 11.965177 60-69
TCATC 2042065 0.82921016 10.718107 110-119
ACAAA 2036500 0.826396 12.995865 130-139
GTCTA 2108115 0.8230433 9.513279 30-34
GGTTC 2522425 0.8198158 11.144165 160-169
ATATG 1814450 0.8181576 5.9530973 50-59
ACATC 2161940 0.81660354 5.7251716 110-119
CCTCC 2875900 0.8143558 10.967885 140-149
TGGCA 2679315 0.81001747 5.6339335 100-109
ATATA 1471685 0.7971446 10.357211 130-139
TTATG 1635620 0.7928698 11.048212 50-59
TGATG 2056460 0.7719372 11.661129 120-129
TATAC 1642710 0.7704058 8.682182 130-139
CTGAC 2440310 0.76733017 9.759216 220-229
GGGAT 2620000 0.76156193 13.590459 100-109
TGACT 1922250 0.7504785 5.0916634 2
CCAGC 2959700 0.74953854 6.187002 80-89
CATCC 2289465 0.74875206 6.821931 110-119
CCCCC 3263565 0.74428904 13.519266 80-89
GTTCA 1906295 0.7442494 13.442375 160-169
TCAAC 1965635 0.7424556 7.713357 110-119
GGTTA 1969915 0.7394506 7.9930778 50-59
ATCTT 1460615 0.7364137 12.915975 1
CGCCG 3493015 0.73640454 10.940314 220-229
GCGGA 3128990 0.732516 9.11894 220-229
GACCG 3007010 0.73217416 9.45141 210-219
GATTA 1618940 0.7299998 5.808388 160-169
TTTAC 1441145 0.72659737 13.404893 45-49
GGGCA 3090555 0.7235181 13.942459 150-159
TCCGG 2759830 0.7224193 12.007425 70-79
TTGGA 1922260 0.7215623 6.396232 60-69
CACAA 2038105 0.71608853 8.493697 130-139
GACCA 2442115 0.71429217 6.1658974 170-179
ATAAA 1414070 0.7124685 7.553016 130-139
TATTG 1463805 0.70958215 6.6672616 240-247
ACGCC 2799375 0.7089366 12.688922 180-189
AACGC 2409410 0.7047264 14.109269 180-189
GGATA 2017915 0.70459104 15.622581 35-39
CAAAC 1988580 0.6986879 14.067934 130-139
ATGCC 2199995 0.6917656 7.931424 50-59
TGCCA 2198095 0.6911682 7.1379333 60-69
CAACG 2351375 0.6877519 14.360307 180-189
AATAC 1575205 0.68717647 11.011858 130-139
TTTCT 1266630 0.6865361 9.705887 4
TATAG 1512905 0.6821873 6.462505 120-129
AAATA 1353280 0.68183994 12.249245 130-139
CAGTC 2152670 0.67688483 5.37202 210-219
GAAAT 1597725 0.6701415 6.956732 110-119
GCGAC 2740165 0.6672004 7.336604 70-79
GCCGC 3160525 0.6663083 12.026569 220-229
AGGCA 2354580 0.6621504 6.42116 80-89
GACGA 2324945 0.65381646 9.94775 220-229
TCCAC 1994325 0.6522288 10.68687 180-189
AATAA 1293710 0.651826 7.066307 130-139
CCCGT 2383265 0.64885265 9.895464 140-149
ATTAA 1188975 0.6440135 6.2775855 110-119
ACTTC 1579095 0.64121455 30.144772 4
CACGC 2531360 0.6410622 11.965561 190-199
TACTT 1251680 0.6310727 11.764911 1
CCGGC 2989930 0.63034314 9.731204 70-79
AGATG 1805265 0.63034046 5.5389137 120-129
CACGA 2149250 0.6286324 9.118579 180-189
CCCTA 1905605 0.6232136 12.13845 110-119
CACCG 2451340 0.62079734 9.127647 45-49
TCAAT 1313645 0.6160794 8.072608 110-119
GGCCC 2910960 0.61369437 10.660407 70-79
CCGTA 1928900 0.6065226 6.2636786 230-239
AAGCA 1795335 0.60648346 5.6764836 120-129
AATGG 1713135 0.59817165 6.739795 120-129
ACGAC 2043295 0.5976417 9.297911 220-229
CTTTG 1417445 0.59492475 7.015645 45-49
TAGCT 1512010 0.5903139 12.03285 50-59
GGTGA 2021885 0.58770627 6.3484144 120-129
GAAGA 1807800 0.5871609 6.291341 15-19
TGATT 1203320 0.58331156 5.9347425 50-59
GGGGA 2573225 0.5791939 9.109539 4
GTTCC 1711195 0.57844794 6.9292808 140-149
ACCTA 1502350 0.56746453 7.084342 130-139
ACCCG 2236295 0.5663376 8.514764 140-149
CGGCA 2320595 0.5650397 11.193057 70-79
TCAGG 1849145 0.55903834 5.476972 150-159
ATTTC 1105640 0.55744225 6.207022 240-247
CCACG 2198325 0.55672175 8.027467 180-189
TAATA 1024205 0.5547651 7.7077885 130-139
TTAGC 1414165 0.5521136 9.487671 45-49
GTCCT 1631405 0.55147594 6.1192117 45-49
GGAGG 2449180 0.5512732 5.290636 6
CTGCG 2099355 0.5495319 11.567056 25-29
CGGTT 1689215 0.54901356 11.032789 160-169
TTCAA 1161530 0.54473984 5.275349 200-209
AAGAT 1287535 0.5400371 5.76772 15-19
GCTAC 1716915 0.5398662 8.010647 50-59
CCTAT 1328195 0.53933287 5.411507 110-119
ACCCC 2017100 0.53130066 9.3219 140-149
CTGTT 1246105 0.52301055 5.610444 240-247
AAATC 1196855 0.52212286 7.4869857 110-119
TATGT 1061035 0.51433855 6.45612 140-149
ACATT 1078205 0.50566167 5.962643 110-119
GGGTA 1739300 0.50556666 5.3833003 100-109
AGTTT 1035110 0.5017714 5.036632 140-149
TGACG 1647410 0.49804926 8.868049 220-229
GGGTT 1570710 0.49082583 5.6975775 100-109
AGTAT 1083815 0.4887054 5.053404 110-119
GTAAC 1336510 0.48537022 6.9092836 120-129
TATCC 1192820 0.4843619 5.4969063 110-119
CGCGG 2387090 0.48385817 6.408025 220-229
TGGCC 1801120 0.47146523 15.49515 2
AACAT 1070380 0.46694872 5.517203 110-119
ATGCT 1190565 0.46481645 7.244359 50-59
TAAAT 845055 0.45772773 7.584237 130-139
GGTTT 1130225 0.4560936 5.2618074 100-109
CAATC 1206610 0.45575824 6.049407 110-119
CCGGT 1739735 0.45539695 8.575686 160-169
TGCTA 1166120 0.4552727 6.5112586 50-59
CATAA 1038455 0.4530216 5.6890063 110-119
TTGGT 1108095 0.44716328 5.4719715 120-129
CCGCG 2116210 0.4461437 6.622214 220-229
ATTCT 861845 0.4345255 7.0585604 2
GGTCA 1434515 0.43368626 5.511181 160-169
TGCGC 1655640 0.43338403 8.549917 25-29
GCCTT 1276175 0.43139488 9.01785 1
ACGAG 1501170 0.42215607 8.005149 180-189
GCGCT 1583305 0.41444954 7.7363076 25-29
GCATG 1366065 0.41299236 8.060817 60-69
CACTT 1008405 0.40947756 6.5942597 3
CTAAC 1080270 0.40803736 5.551462 110-119
CAATG 1106440 0.40181744 5.017668 120-129
ATGGC 1328860 0.40174443 7.4522247 1
CGGTG 1591605 0.40056747 5.0990124 8
CCTAA 1058510 0.3998182 7.6028996 110-119
CCGGG 1969560 0.3992257 5.318412 80-89
AGCTT 1013780 0.39579663 7.5317235 1
GAGGC 1655145 0.38747972 6.13269 35-39
CATGC 1224525 0.38503924 6.731883 60-69
TTTGA 793435 0.38461906 5.8325987 45-49
GGTAA 1100840 0.3843779 7.266477 120-129
TTCTT 679405 0.36824965 20.192448 1
TGCTG 1083430 0.3521267 13.914653 1
TGGGC 1398855 0.35205704 9.732159 1
CGAGC 1444555 0.35173345 6.7974114 180-189
CTCGG 1300145 0.34032887 5.521302 6
CTCTT 728905 0.31819504 9.459664 1
GCTTT 739240 0.3102711 5.1547947 45-49
CGCTT 908860 0.30722868 5.9680266 1
GCGAG 1311165 0.30695185 5.4911895 240-247
CGGGA 1297365 0.3037212 5.0821958 80-89
AACTT 642140 0.30115384 10.971093 3
TCACA 795860 0.3006106 5.1207623 5
GACTT 762630 0.29774347 6.8392124 1
ATTGT 613695 0.29748973 5.432292 240-247
TTCCT 652380 0.28478897 6.4987707 4
GAGGT 952145 0.27676237 6.683643 7
CCCTT 774545 0.2723191 5.6713433 3
GTCTT 641345 0.26918295 11.899781 1
TGTAA 586950 0.26466292 7.871288 25-29
TCGGT 810550 0.2634377 6.618145 7
AATCT 557245 0.26133937 7.3715544 2
TAACT 556720 0.26109317 6.8762946 2
TTGGC 800660 0.26022333 7.69251 1
CGGGG 1321255 0.25749528 9.084391 3
CCTCG 905805 0.24660873 6.00321 5
TGCTT 536585 0.22521345 11.448422 1
TCGCA 582760 0.18324284 6.7846622 7
TAGGC 512475 0.15493278 7.111041 1
TTGCT 314755 0.1321078 5.783847 4
Produced by FastQC (version 0.10.1)