FastQCFastQC Report
Wed 24 May 2017
HCGTCBCXY_s1_2_illumina12index_15_SL248344.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename HCGTCBCXY_s1_2_illumina12index_15_SL248344.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10260331
Filtered Sequences 0
Sequence length 251
%GC 54

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
TTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 42066 0.4099867733311917 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 35266 0.34371210831307486 No Hit
TTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 35071 0.3418115848309377 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33824 0.32965798081952713 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 33289 0.32444372408648414 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 30615 0.29838218669553646 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 29118 0.2837920141172834 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 27920 0.27211597754497396 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27668 0.26965991642959664 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 27227 0.26536180947768645 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 27206 0.26515713771807164 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 26716 0.2603814633270603 No Hit
GTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 26028 0.25367602663110966 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 25624 0.24973853182709213 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25092 0.2445535139168512 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 25062 0.24426112568883013 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24908 0.24276019945165514 No Hit
TGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24745 0.24117155674607377 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 24632 0.24007022775386094 No Hit
TCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 24490 0.2386862568078944 No Hit
CTCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 23750 0.23147401385004052 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 23423 0.22828698216461046 No Hit
TACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 23323 0.22731235473787345 No Hit
ATCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 22885 0.22304348660876533 No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21761 0.21208867433224135 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 21449 0.20904783676082184 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 21291 0.2075079254265774 No Hit
CTCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 20808 0.2028004749554376 No Hit
GACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20414 0.1989604428940938 No Hit
GGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 20242 0.1972840837201061 No Hit
ACCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 20066 0.19556873944904896 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19572 0.19075407996096813 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 19498 0.19003285566518271 No Hit
AGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 19422 0.1892921388208626 No Hit
AGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 19367 0.18875609373615723 No Hit
CGCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 18737 0.18261594094771408 No Hit
GACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 18709 0.1823430452682277 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 18599 0.181270955098817 No Hit
TTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 18436 0.17968231239323565 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 18420 0.17952637200495775 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 17858 0.17404896586669572 No Hit
AACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17658 0.17209971101322172 No Hit
AACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17286 0.16847409698576002 No Hit
CCCTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 17208 0.16771388759290515 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 17089 0.1665540809550881 No Hit
GGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCAG 16832 0.16404928846837397 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 16645 0.16222673518037575 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 15958 0.15553104475869248 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 15933 0.15528738790200824 No Hit
CGCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 15693 0.1529482820778394 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGATACACCTTCACCGG 15658 0.15260716247848144 No Hit
AGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGTTACACCTTTACCAG 15494 0.15100877349863276 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14788 0.14412790386586943 No Hit
ACCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 14561 0.14191549960717642 No Hit
CACTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14519 0.14150615608794687 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA 14268 0.13905984124683696 No Hit
TGGGCCTCAGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG 14102 0.13744195971845352 No Hit
CACTTCGGAGACCCTGTCCCTCACCTGCACTGTCTCTGGTGGCTCCATCA 14078 0.13720804913603663 No Hit
TGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13702 0.13354345001150547 No Hit
GTCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 13125 0.12791984975923293 No Hit
TCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12988 0.1265846101846032 No Hit
GCCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12776 0.12451840003992075 No Hit
ATCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 12379 0.1206491291557748 No Hit
CCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCA 11866 0.11564929045661393 No Hit
GGCTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10781 0.10507458287651733 No Hit
TACTTCGGAGACCCTGTCCCTCACCTGCGCTGTCTATGGTGGGTCCTTCA 10693 0.10421691074098878 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGGA 20427495 7.5206137 66.20212 35-39
TATTA 10134295 7.070439 122.71463 240-247
TGGAT 15442285 7.002841 78.00887 35-39
TCACC 15863640 6.384055 73.87546 160-169
TCTCC 14146540 6.137035 87.71741 20-24
CTGTG 14876885 5.9042506 87.540474 25-29
TCCCT 13370120 5.8002095 72.81134 15-19
AGTGG 15863710 5.5861816 39.111904 100-109
CTGAG 15080020 5.55188 50.068512 15-19
TGTAT 9265610 5.411144 57.84191 240-247
TTACT 8470515 5.171931 109.01579 240-247
TGGGT 12820445 4.8666267 45.14274 100-109
ATTAC 8562570 4.849896 102.210495 240-247
CCTGA 12564930 4.836442 51.837826 15-19
CTCTG 11383650 4.72348 73.32695 35-39
TTCAC 9366520 4.642977 73.70231 40-44
CTCCA 11447375 4.606803 41.352974 80-89
ACACG 12652125 4.517673 48.637245 230-239
GACAC 12627810 4.5089912 45.98341 230-239
TGAGA 10407960 4.3783827 92.83105 15-19
TCTGG 10830065 4.2981725 134.36937 35-39
ATTCA 7564730 4.284713 109.97586 40-44
AAGGG 12899245 4.2136655 38.40162 90-99
TACTG 8884080 4.2121415 77.18668 240-247
CCCTG 12409720 4.1804013 85.9632 10-14
CAGCC 13195090 4.1233835 55.67376 30-34
AGCCT 10693905 4.116255 51.03765 30-34
CCAGG 13646420 4.078799 52.90096 80-89
GAAGG 12429735 4.0602946 30.612417 150-159
TGGAG 11528200 4.0594935 38.18088 90-99
GAGAC 11739690 4.009407 84.29561 8
ACCAT 8683830 3.993144 65.62191 160-169
TCCAG 10295620 3.962949 40.599945 80-89
GGGTC 12761645 3.9328468 126.19335 8
TCAGT 8265630 3.918921 92.09539 7
CACCA 10451560 3.9017572 56.26858 160-169
ACTAC 8363815 3.8459895 34.460945 130-139
GTATT 6566960 3.8351243 67.035324 240-247
CTGGG 12290190 3.787555 133.91261 3
GACTC 9792225 3.7691836 52.055336 20-24
CACCT 9357910 3.7659328 44.030766 20-24
TACTA 6646920 3.7648587 27.911743 130-139
GATTC 7803245 3.6996934 58.55843 40-44
ACTGT 7775400 3.6864915 66.34966 240-247
AGACT 8354600 3.674528 68.251396 15-19
TGAAG 8667725 3.646307 39.0852 150-159
GGACA 10398830 3.5514686 27.822031 220-229
GCTGG 11521335 3.5506113 33.094765 90-99
CCTGT 8535580 3.5417147 62.57681 25-29
CAAGA 8637010 3.523909 42.98633 180-189
ACTGG 9441260 3.4759066 43.227913 60-69
ACTCT 6993475 3.4666607 68.419754 20-24
GAGTG 9697090 3.4146934 37.96823 100-109
TCCTG 8083040 3.35394 64.119255 20-24
CTCCT 7605015 3.2991986 94.48548 20-24
TATCT 5379425 3.284572 48.324932 200-209
GGTCC 10165835 3.275452 144.3463 9
GGATT 7092565 3.21637 55.863422 40-44
AGACA 7833535 3.1960905 36.718746 170-179
CTGCA 8295770 3.1931744 44.484566 25-29
CATCT 6392115 3.168567 32.78633 170-179
AGCTG 8577985 3.1580822 35.774788 60-69
CCATC 7757045 3.1216924 34.325684 45-49
GTCTC 7489725 3.1077526 32.942764 30-34
GTCCC 9144520 3.0804694 75.92675 10-14
CCTCA 7406120 2.980468 78.81389 5
GGAGT 8365205 2.945689 36.553104 90-99
GGCTG 9502830 2.9285543 34.18522 90-99
ATCTC 5818880 2.8844144 48.023994 170-179
AGGGA 8823630 2.882325 39.43616 80-89
GTGTA 6355580 2.8821588 34.781578 230-239
AAGAA 6142380 2.863571 35.955948 180-189
TGTGC 7079555 2.809692 57.711586 25-29
AGTAG 6613035 2.7819471 27.670683 50-59
GAGCT 7508325 2.7642746 28.98478 60-69
GTGAA 6564970 2.7617276 38.14246 150-159
GGAAG 8431820 2.754337 29.607676 80-89
AAAAA 4934415 2.748177 19.702803 90-99
ATCTG 5779330 2.74011 36.442535 200-209
CAGGG 9397675 2.6866195 35.94089 80-89
GACAA 6570800 2.6808934 24.31115 180-189
ACCTT 5346215 2.6501148 69.55597 40-44
GAACA 6481430 2.6444302 34.145824 210-219
CTATG 5552850 2.632731 27.231361 50-59
GGGAA 8026770 2.622023 33.262524 80-89
ATGAA 5113215 2.5696852 26.786583 210-219
CACGG 8535740 2.55126 43.99055 230-239
CCCTC 7228000 2.545669 27.574202 20-24
CGCCA 8144745 2.545182 39.85394 70-79
GAGTC 6850850 2.5222178 27.10086 150-159
TGCAA 5697020 2.5056689 35.3026 200-209
AGGGC 8703320 2.4881163 32.354183 150-159
AGAAC 6077550 2.4796467 29.373985 190-199
CAGTG 6663645 2.4532964 71.50742 8
CCAAG 6862800 2.4504888 38.13994 180-189
AGGCT 6583165 2.4236665 19.95384 80-89
ACGGC 8106990 2.4231102 48.38746 230-239
TATAT 3464200 2.4168842 23.811623 240-247
CTCTC 5554545 2.409666 98.81043 20-24
ATCAG 5469795 2.4057302 25.865328 45-49
TCTGT 4683870 2.393922 23.941431 210-219
CTACA 5200210 2.391248 18.779501 190-199
CACTG 6168515 2.3743598 19.169628 30-34
CCGCC 8673195 2.3719773 34.475883 70-79
GCAGC 7905300 2.3628268 34.36511 30-34
GTATC 4983365 2.3627253 22.759436 200-209
GTGGA 6708280 2.362226 25.830145 100-109
CTGTC 5679945 2.3568106 47.70873 30-34
TGAAC 5311310 2.3360255 37.214306 210-219
GTGGT 6078615 2.3074355 30.55588 120-129
ACAGC 6439845 2.2994647 26.645395 210-219
CTACT 4618250 2.2892635 22.124 50-59
GGGCT 7424010 2.2879097 42.140297 90-99
CTTCA 4584085 2.272328 49.94551 45-49
TATGC 4773675 2.2633066 20.62608 50-59
GGCCG 9041990 2.2622457 24.73281 230-239
CAAAT 4300165 2.2594295 34.41847 200-209
TGGTG 5924695 2.2490077 33.339836 35-39
GCCTC 6672945 2.2478817 69.81341 4
TCCAA 4888260 2.2478018 41.837894 180-189
CAGGC 7480045 2.2357216 32.89175 70-79
TATCA 3901420 2.20979 16.439411 170-179
GCTCC 6542370 2.2038956 34.257706 80-89
GCCTG 6835650 2.2024593 30.759268 210-219
GGGGG 9575720 2.191753 95.78021 5
ACAAC 5111620 2.1804576 19.695585 180-189
AATGA 4323715 2.172916 25.367731 210-219
AAATG 4320485 2.1712925 32.58435 200-209
CCTTC 4908795 2.1295273 55.126583 45-49
CAGTA 4827145 2.1230795 20.782719 45-49
CCTCT 4888520 2.1207316 49.140263 35-39
GTGGG 7132915 2.1025174 29.950573 100-109
TGCAG 5685010 2.093001 43.235832 30-34
TACAT 3686055 2.0878055 17.435375 190-199
GCTGT 5217430 2.0706627 19.333641 230-239
GCTAT 4346320 2.0606878 32.952103 50-59
GTAGT 4543605 2.0604556 24.38051 120-129
GCAGA 6005135 2.0509086 23.992058 140-149
CTCAC 5094495 2.0501938 55.020775 20-24
TCTAT 3343170 2.0412743 19.48737 110-119
AGAGT 4802585 2.0203338 29.131008 150-159
AACAG 4913200 2.0045907 32.792797 210-219
CAGAG 5807245 1.9833239 32.204803 170-179
TGCAC 5146490 1.9809663 25.83011 25-29
GGCTC 6116795 1.9708432 19.636663 40-44
TGTGA 4342180 1.9691124 18.304089 210-219
TCCGC 5840745 1.9675432 42.270542 70-79
AACTA 3714605 1.9517596 35.049458 130-139
TCTCT 3648195 1.949445 32.54059 35-39
AGCAC 5403735 1.9295026 20.050848 130-139
GGTGG 6541050 1.9280577 17.912315 40-44
TGAGC 5222720 1.9228035 17.186384 200-209
CTGTA 4048775 1.9196151 23.832466 190-199
ATGGA 4562745 1.919439 11.828987 120-129
CCGTG 5939780 1.9138086 19.55531 230-239
CAGAC 5353795 1.9116707 26.193493 140-149
GGTCT 4808665 1.9084347 34.888027 15-19
TGGTA 4207400 1.9079918 29.992884 120-129
TTCAG 4020435 1.9061785 46.669323 45-49
ATATC 3347535 1.8960658 13.0460005 160-169
CCAGA 5286430 1.887617 33.93283 170-179
CATAT 3329430 1.8858109 13.846257 160-169
TGGGG 6395425 1.8851328 124.209946 4
GCCAG 6302655 1.8838098 35.44175 70-79
ACCCT 4636185 1.8657544 43.670082 10-14
AGAGA 4735025 1.8478032 27.632414 170-179
ACTAT 3256395 1.8444433 16.421698 130-139
AGGAC 5394940 1.8425112 20.432312 220-229
CCTGC 5462620 1.840166 48.750965 20-24
GGATC 4996940 1.83968 17.803745 70-79
TCCAT 3707000 1.8375574 26.299297 45-49
CTATT 2999110 1.831198 19.140818 110-119
GTCAC 4745560 1.826642 37.037952 160-169
GCACA 5107430 1.8237015 17.793573 130-139
TGTCT 3547120 1.8129299 48.69802 30-34
GATGG 5139075 1.8096527 20.140486 100-109
TTCCA 3641965 1.8053195 22.771547 180-189
ACCAG 5041380 1.8001171 13.538085 190-199
CAGTT 3791285 1.7975333 23.832699 190-199
GCCGA 6003475 1.7943875 19.297184 220-229
TGGGA 5064790 1.7834944 16.059551 100-109
GAGGA 5458990 1.7832325 26.548141 220-229
GCTGA 4823905 1.775975 26.10374 200-209
TCAGC 4604715 1.7724285 11.471248 45-49
ATACT 3116455 1.7651806 26.03983 130-139
CACAG 4932655 1.761295 15.315152 140-149
ACCTG 4549835 1.7513041 30.73152 25-29
TAGTG 3839195 1.7410164 25.434391 120-129
CAAGG 5079925 1.7349254 16.571869 80-89
TAGTA 3184900 1.7254258 16.460339 120-129
TTCTG 3365005 1.7198511 41.406258 30-34
CCTGG 5321815 1.714699 34.38396 2
GCAAA 4201210 1.7140981 31.657307 200-209
AGTCA 3877030 1.7051989 27.962427 160-169
GTGCA 4623095 1.7020447 50.226112 25-29
AGTTC 3556330 1.6861359 21.068565 190-199
TACAA 3203510 1.6832156 17.01243 130-139
CTGAA 3817400 1.6789726 22.236078 200-209
CTCAG 4360355 1.6783705 75.02194 6
TGACC 4350310 1.6745038 14.088981 210-219
CATGA 3804685 1.6733801 22.699602 60-69
GCCGT 5149100 1.65905 28.691975 230-239
ATGCA 3746195 1.647655 16.268444 60-69
AACAC 3858725 1.6460116 22.17492 190-199
AGCTA 3721760 1.636908 25.022783 50-59
TCTGC 3933060 1.6319661 33.643646 200-209
GGAGC 5697325 1.6287587 12.717036 200-209
GGATG 4613345 1.6245245 21.486376 100-109
TCTCA 3256465 1.6142275 25.923588 100-109
AGGGG 5897230 1.6125232 32.55725 90-99
ATTGG 3549240 1.6095263 19.86431 100-109
GGGCC 6429335 1.6085769 27.330233 2
GGGGC 6634255 1.5875964 28.685068 90-99
CGATT 3345350 1.5861055 34.927982 160-169
CAGCT 4077015 1.5693082 12.652756 50-59
CTCCC 4435435 1.5621402 13.883871 190-199
TCTGA 3289855 1.5597942 20.624435 220-229
ACAAT 2931880 1.5404935 22.758482 180-189
GCACT 3967425 1.5271252 24.843645 25-29
GTTCT 2972615 1.5193009 22.353254 190-199
GTCCG 4698770 1.5139527 34.705387 70-79
CGTCC 4481175 1.509551 18.644758 180-189
TTCTC 2806310 1.4995763 21.411627 190-199
CCATG 3892055 1.4981142 15.059838 160-169
CGGCC 5702135 1.491561 31.293333 230-239
AGTGA 3543815 1.490799 81.44181 9
GGCTT 3718100 1.4756175 20.56991 25-29
TTAGT 2522690 1.4732585 24.92344 110-119
AGTTA 2717210 1.4720538 15.282348 50-59
CTGGT 3701460 1.4690137 30.777416 35-39
ACTCC 3648605 1.4683195 17.15093 140-149
GAGCA 4288450 1.4646161 7.369014 200-209
AAGAG 3734465 1.4573432 17.969133 150-159
CATCA 3164355 1.4550868 30.171907 45-49
CTTTA 2369880 1.4470025 41.6804 45-49
TACGC 3751425 1.4439833 24.445526 140-149
CGTGT 3586445 1.423367 23.087814 230-239
GGGGT 4791585 1.4123806 118.61746 7
GTTAC 2965990 1.4062425 15.200395 50-59
GTGAC 3791910 1.3960346 14.719313 210-219
CTACG 3573060 1.375328 16.155436 130-139
GGCAG 4806690 1.3741428 13.136842 150-159
CTCAA 2979570 1.370116 18.001879 150-159
GTCCA 3551895 1.3671811 27.980175 180-189
GAGAG 4162235 1.3596348 25.345753 220-229
TAGCA 3083385 1.3561373 11.33853 130-139
CAACC 3629065 1.3547958 14.917062 140-149
GGCCT 4204240 1.3546143 66.51988 3
CAACT 2940215 1.352019 17.050453 130-139
AACCC 3609535 1.347505 19.58958 140-149
GAACT 3042265 1.3380518 14.345555 190-199
AGAGC 3897235 1.3310063 24.28769 220-229
AGCAG 3884410 1.3266262 12.169797 50-59
TGTGT 2712625 1.3260719 14.940036 240-247
ATCAT 2336615 1.3234739 15.448453 110-119
TCACT 2666800 1.321931 13.247048 190-199
TGGAA 3142160 1.3218324 19.03049 120-129
AAGCT 2983685 1.3122871 15.850199 200-209
TTATT 1742505 1.3105135 20.491528 240-247
CGACA 3663315 1.3080537 15.640332 70-79
CAATT 2303260 1.3045816 23.807081 180-189
ATACA 2479555 1.302829 20.72939 35-39
ATCTA 2299370 1.3023782 19.890486 110-119
ACAGT 2960395 1.3020437 11.934682 120-129
TGTCC 3123940 1.2962334 53.813904 15-19
CCTTT 2425040 1.2958413 48.211823 45-49
GTGGC 4199720 1.2942575 14.112624 40-44
CACGT 3354530 1.2912121 16.248154 180-189
CCATA 2802070 1.288495 14.274926 160-169
TCCGT 3104820 1.2882999 13.844636 150-159
GTGCG 4155630 1.2806699 16.660313 240-247
TCAAG 2901980 1.2763516 22.24998 150-159
ACAGA 3125955 1.2753929 19.280048 140-149
AATTC 2240045 1.2687762 23.772528 180-189
TTACA 2229745 1.2629422 9.347754 120-129
AGATC 2870220 1.2623827 12.013634 210-219
ATATT 1807295 1.2609037 25.418344 240-247
ATTAT 1806630 1.2604398 9.655871 50-59
ACATG 2860750 1.2582178 13.801267 190-199
CCTAC 3116580 1.2542151 16.782255 190-199
AGACC 3505395 1.2516656 97.73058 9
ACATA 2378015 1.249477 20.073368 130-139
GATCT 2628955 1.2464465 9.19299 220-229
AACCA 2918350 1.244877 16.744778 190-199
TCGGA 3373890 1.2421358 91.00058 5
CCAGT 3214815 1.2374338 18.262682 190-199
TGCGA 3350440 1.2335024 13.723669 240-247
CGGCT 3820275 1.2308999 21.341843 230-239
ACACA 2857710 1.2190099 7.574537 130-139
ACGTC 3142340 1.2095368 17.971317 180-189
CTTCT 2260290 1.2078058 22.604052 30-34
ATCCG 3136780 1.2073967 23.235954 70-79
AGTCG 3270555 1.2040917 15.575967 150-159
GATTG 2642020 1.1981157 22.867046 100-109
TGGAC 3210215 1.181877 15.185466 80-89
TGAGT 2585925 1.1726775 19.939518 90-99
CGCTG 3633370 1.1706787 12.283823 190-199
TACCA 2536640 1.1664404 10.447804 45-49
GTCGA 3155865 1.1618674 12.156724 150-159
CTTCG 2799350 1.1615496 103.59997 3
ATTAG 2132480 1.1552752 24.607487 110-119
GGGTG 3918760 1.1551043 14.128527 60-69
ATTCC 2322185 1.1511055 22.27191 180-189
GATCC 2985125 1.1490222 23.086355 70-79
ACGCA 3215650 1.1482068 20.720655 140-149
AAGGC 3333740 1.1385583 27.395332 25-29
GAAGT 2694510 1.1335166 12.890598 140-149
GGACT 3071465 1.1307946 12.784502 90-99
AGGCC 3767775 1.1261557 13.758104 2
GACCC 3591880 1.1224401 42.976566 10-14
TCGAG 3023935 1.1132959 11.852062 150-159
AGCTC 2874540 1.1064563 11.952097 210-219
TTGGG 2906015 1.1031201 14.836311 100-109
GAACC 3082660 1.1007203 10.112984 180-189
CGCAG 3674780 1.0983604 16.387255 140-149
GCAAG 3212180 1.0970423 16.470284 25-29
CCGAT 2849855 1.0969548 23.616276 160-169
GCCCC 4011010 1.0969456 12.329935 80-89
TTCGG 2761370 1.0959162 98.817986 4
GTATA 2020045 1.0943633 9.949389 240-247
AGGTC 2972280 1.0942783 17.437798 10-14
ACAAG 2652580 1.0822555 16.458279 80-89
GCTTC 2600375 1.0789877 27.35185 2
GTAGA 2557960 1.0760733 16.302757 170-179
CACAT 2335405 1.073905 13.49913 130-139
CCCCT 3047600 1.073351 9.465738 140-149
GCACC 3413080 1.0665662 10.892644 130-139
CTTGA 2237665 1.0609273 21.30951 90-99
CTCCG 3149080 1.0608151 9.965098 140-149
TTGAG 2331220 1.0571728 19.701895 90-99
ATGGG 2978670 1.0488967 19.350367 100-109
GGAGA 3206845 1.0475473 80.49266 7
TACAG 2379770 1.0466726 8.891766 120-129
TAGAC 2376225 1.0451136 16.690495 170-179
ATCAA 1980830 1.0407847 12.11782 110-119
GAAGC 3039035 1.0379088 12.386385 200-209
CAGCA 2897890 1.0347447 12.087977 45-49
ACACC 2769155 1.0337758 12.730242 40-44
TGGCT 2603070 1.0330911 30.545822 40-44
ATGAG 2449760 1.0305561 19.42563 60-69
CGTGA 2794180 1.0287088 20.53025 150-159
CTATA 1810085 1.025244 12.629127 50-59
GGGAG 3747335 1.0246615 14.584349 5
GAGCC 3427415 1.024425 17.921621 220-229
ATGGT 2244280 1.0177468 9.285157 35-39
GCTCT 2431825 1.0090503 16.746832 210-219
CGAGG 3525445 1.0078588 14.626337 220-229
ACTCA 2191680 1.0078151 12.181104 190-199
GACAG 2942415 1.0049106 14.981934 70-79
ACCGC 3215560 1.0048425 10.909634 220-229
AGCCG 3346810 1.000333 18.923986 220-229
TGAGG 2839205 0.9997861 11.713032 220-229
GTAGC 2708225 0.9970637 10.971602 50-59
TTATA 1416185 0.9880361 13.006926 110-119
TTTCA 1616650 0.9870949 7.23261 100-109
TAGTT 1687115 0.9852802 14.221504 50-59
AACTC 2137675 0.98298156 16.165321 190-199
AAACT 1866060 0.9804812 16.217867 130-139
CGTAT 2065995 0.9795345 7.377042 240-247
GCCAA 2728725 0.9743413 14.679489 180-189
ATGAC 2208420 0.97130936 10.35153 160-169
ATGAT 1786020 0.9675798 9.002951 120-129
ACCAA 2267775 0.96736205 16.380152 130-139
CCGTC 2870875 0.9670975 10.247068 140-149
TTTAG 1650000 0.9636049 29.364122 45-49
CCGAG 3202560 0.957218 17.810156 220-229
CGAGT 2586000 0.95206517 14.610813 160-169
GCAGT 2584245 0.95141906 5.988346 50-59
CCAAC 2546595 0.95069027 13.677441 130-139
AAGGT 2248525 0.9459013 20.011097 15-19
GGTAT 2084465 0.9452731 9.248439 110-119
GCCAT 2449410 0.94281703 7.6849675 60-69
GATAC 2134260 0.9386922 22.839725 35-39
CATAC 2031735 0.9342664 13.801472 130-139
CGGAC 3125145 0.93407923 10.675558 220-229
GGGAC 3263615 0.93300647 9.821709 90-99
TTTAT 1237290 0.93054837 10.8983965 240-247
CATTA 1639970 0.9288897 12.013739 110-119
AACTG 2109875 0.92796725 11.516887 60-69
AGCCC 2951285 0.9222582 11.310121 80-89
TATGG 2026500 0.91898686 11.995204 35-39
AATCA 1744040 0.9163684 13.427179 110-119
TACAC 1976100 0.9086834 20.687689 35-39
ACAGG 2658545 0.90796167 14.527171 70-79
CAGAA 2224660 0.90766364 17.227194 140-149
CCCAG 2887535 0.9023368 14.441277 80-89
TAACA 1713740 0.90044796 8.19499 120-129
CCCCA 2753045 0.89946127 16.662523 80-89
TCATA 1587635 0.8992469 8.603366 110-119
GAGAT 2123110 0.8931421 11.518235 210-219
TATTT 1184120 0.89056 10.890963 240-247
GTCTG 2231790 0.8857397 15.866962 3
GTTAT 1516375 0.88556755 10.69256 110-119
TGGTT 1803595 0.8816908 8.995139 50-59
AGTCT 1857705 0.88077956 16.147287 8
GACTG 2392170 0.88070446 12.4192705 90-99
TCCTT 1647110 0.8801477 10.746716 1
ACGCT 2274725 0.875578 14.975666 190-199
CGGAG 3062385 0.87547857 70.61775 6
AAGTT 1606200 0.87016195 16.594175 140-149
GATGA 2054690 0.8643594 6.6536736 60-69
AGAAG 2180725 0.85100937 14.961147 140-149
ATAGT 1561745 0.8460784 15.02878 120-129
GTTTC 1645360 0.84094197 5.041911 100-109
ATCCA 1826515 0.83989877 9.490711 180-189
GGTTC 2104675 0.8352909 11.410608 160-169
TCTTC 1562935 0.83516806 44.903557 2
GGTAG 2368010 0.8338614 11.637945 120-129
TTACC 1678440 0.83200157 9.877189 45-49
GTCTA 1747235 0.82840335 9.790832 30-34
CTATC 1662835 0.82426614 8.1433 110-119
GATCA 1873280 0.82390773 8.965214 110-119
GCCTA 2140025 0.8237297 16.051134 190-199
ATATA 1272030 0.8232574 10.2796 130-139
TCATC 1657000 0.82137376 10.286589 110-119
CATGG 2229045 0.82064813 8.387507 200-209
TGGCA 2219410 0.8171009 5.7851853 100-109
ACAAA 1667080 0.8125601 12.23907 130-139
ATATG 1488855 0.80659014 5.3601193 50-59
CCTCC 2289700 0.80642194 10.47449 140-149
ACATC 1744305 0.8020956 5.5128293 110-119
GGTGC 2598865 0.80091065 11.292262 60-69
GCTTG 2015940 0.80007434 16.397785 90-99
TTATG 1369210 0.7996227 11.24465 50-59
TGATG 1750060 0.79362553 11.996818 120-129
CGCCG 2935405 0.7678415 10.772643 220-229
TATAC 1353345 0.7665435 8.678218 130-139
GTTCA 1605265 0.7610921 13.709404 160-169
ATAAA 1250960 0.7510469 7.664263 130-139
CTGAC 1937900 0.7459287 9.23208 220-229
CCAGC 2386830 0.7458696 6.191109 80-89
CCCCC 2595400 0.74210185 13.400433 80-89
CATCC 1838805 0.7399961 6.5372314 110-119
ATTAA 1139790 0.7376717 6.3598895 110-119
ATCTT 1199315 0.7322782 11.80387 3
TTGGA 1606255 0.72841215 6.4393544 60-69
GCGGA 2541605 0.7265973 8.907928 220-229
TATTG 1243825 0.7263975 6.5364594 240-247
GGGAT 2062470 0.7262697 12.786525 100-109
TCAAC 1578875 0.7260248 7.1743617 110-119
AAATA 1207625 0.7250295 12.303422 130-139
ACGCC 2316175 0.7237903 12.972321 180-189
TTTAC 1176575 0.7183937 13.348857 45-49
GATTA 1325460 0.7180705 5.53602 50-59
TTTCT 1090005 0.71744066 9.509251 4
GGTTA 1579695 0.7163676 7.9136186 50-59
AACGC 2004610 0.71578276 14.276414 180-189
GACCG 2377330 0.71056366 9.096642 210-219
ATGCC 1842770 0.7093116 8.034185 50-59
TCCGG 2193390 0.7067146 11.569564 70-79
TGCCA 1834705 0.7062072 7.375542 60-69
AATAC 1337320 0.7026661 11.131943 130-139
CAACG 1952790 0.69727945 14.5588 180-189
CACAA 1621250 0.69157463 8.183412 130-139
GACCA 1931155 0.6895543 5.9233804 170-179
GGGCA 2409880 0.68893963 13.136277 150-159
GAAAT 1359150 0.68305117 6.9710183 110-119
CAGTC 1769085 0.68094903 5.5019464 210-219
GGATA 1618300 0.68078053 15.070489 35-39
TATAG 1249380 0.6768541 6.0353975 120-129
AATAA 1126155 0.6761169 7.172906 130-139
CCGTA 1738520 0.6691841 6.2512717 230-239
CAAAC 1568490 0.6690689 13.160668 130-139
AGGCA 1942875 0.66354185 6.599602 80-89
GCCGC 2522875 0.65993214 11.81685 220-229
CACGC 2106435 0.6582478 12.242113 190-199
CCCGT 1951175 0.6572827 9.991931 140-149
AGATG 1556360 0.6547238 5.7971334 120-129
ACTTC 1297690 0.643264 30.293781 4
TCCAC 1595750 0.64218277 10.087297 180-189
GAAGA 1641705 0.6406614 6.3143506 15-19
GCGAC 2128425 0.63616806 7.035476 70-79
GACGA 1858370 0.6346813 9.373375 220-229
TCAAT 1112515 0.6301358 8.1298895 110-119
TACTT 1020530 0.6231156 11.247155 1
CACCG 1987445 0.6210641 8.727367 45-49
AAGCA 1502170 0.61288697 5.768785 120-129
CCGGC 2329700 0.6094016 9.456802 70-79
AGTAA 1206805 0.606489 5.114897 50-59
GGCCC 2309570 0.60413593 10.135167 70-79
TAGCT 1268820 0.60157603 12.134902 50-59
AATGG 1428100 0.6007679 6.475265 120-129
GGTGA 1702555 0.5995307 6.4428673 120-129
CACGA 1678215 0.59923744 8.589184 180-189
CCCTA 1488105 0.59886277 11.425829 110-119
TGATT 1018875 0.5950261 6.000085 50-59
CTTTG 1160795 0.5932813 7.1561494 45-49
GGGGA 2131655 0.58287424 9.484529 4
ACGAC 1627435 0.58110553 8.7057495 220-229
ATTTC 949005 0.57944393 6.4806695 240-247
AAGAT 1150355 0.5781197 5.7791095 15-19
TAATA 888590 0.5750951 7.8588443 130-139
ACCCG 1827400 0.57105106 8.556382 140-149
ACCTA 1240045 0.57021827 6.9002533 130-139
GTCCT 1361810 0.56506324 6.282104 45-49
GTTCC 1360795 0.5646421 6.577707 140-149
CGGTT 1418105 0.5628091 11.312783 160-169
TTAGC 1178585 0.5587936 9.462127 45-49
TTCAA 985650 0.55827856 5.2945676 200-209
CTGCG 1713605 0.5521268 11.892314 25-29
TCAGG 1498065 0.5515296 5.141236 150-159
GGAGG 2005575 0.5483992 5.211012 6
CGGCA 1834130 0.54820585 10.71563 70-79
CTGTT 1065630 0.54464257 5.5060863 240-247
TATGT 927635 0.5417416 6.654275 140-149
CCACG 1712785 0.5352347 7.625511 180-189
AAATC 997580 0.524157 7.57182 110-119
GCTAC 1360635 0.52373016 7.66838 50-59
ACATT 919485 0.5208023 6.087624 110-119
ACCCC 1585875 0.5181292 8.840186 140-149
GGGTT 1316440 0.49971914 5.8569965 100-109
AACAT 944815 0.49643278 5.73694 110-119
GTAAC 1103545 0.4853622 6.5761476 120-129
TGGCC 1505810 0.48517492 14.546159 2
CGCGG 1932315 0.48345232 6.3942056 220-229
TATCC 971015 0.48133147 5.390668 110-119
TGACG 1302715 0.47960928 8.259968 220-229
CAATC 1025285 0.47146374 6.1565676 110-119
TAAAT 727870 0.47107714 7.6950727 130-139
CCGGT 1453975 0.46847355 8.872645 160-169
CATAA 886290 0.4656821 5.9449034 110-119
ATGCT 971975 0.46083516 6.8816047 50-59
GGTTT 939135 0.45909792 5.363613 100-109
TGCTA 945880 0.44846296 6.1384206 50-59
ATTCT 733770 0.4480256 7.389062 2
CCGCG 1709150 0.44707853 6.6389117 220-229
TGCGC 1366045 0.44014236 8.782363 25-29
TTGGT 892930 0.4365105 5.0992284 120-129
GCCTT 1043310 0.43290624 8.672174 1
GCATG 1175730 0.43285832 8.39774 60-69
GGTCA 1153475 0.42466488 5.3760824 160-169
CACTT 848755 0.42072722 7.266204 3
ATGGC 1123505 0.41363108 6.9086595 1
GCGCT 1277195 0.4115147 7.9259806 25-29
ACGAG 1184835 0.4046517 7.459521 180-189
CATGC 1047030 0.40301856 7.0815187 60-69
AGCTT 842965 0.3996686 7.0269194 1
CCGGG 1586230 0.3968642 5.3074207 80-89
CTAAC 861550 0.39617237 5.1754575 110-119
TTTGA 675330 0.39439473 5.937075 45-49
CCTAA 833460 0.38325557 7.150568 110-119
CTCGG 1187820 0.38271794 5.0001435 6
GAGGC 1319710 0.37728038 5.6468997 35-39
TTCTT 571510 0.37616754 19.3003 1
GGTAA 888230 0.37365735 6.897844 120-129
ATGTG 787345 0.35704896 5.015015 140-149
TGCTG 892035 0.3540256 13.620406 1
TGGGC 1126330 0.3471091 9.128064 1
CGAGC 1126290 0.33663848 6.395176 180-189
GCTTT 622905 0.31836617 5.163476 45-49
CTCTT 594700 0.31778315 8.946129 1
TCGGT 790020 0.3135385 5.8604193 7
TCACA 681285 0.31327993 5.1055374 7
CGGGA 1059585 0.30291554 5.013672 80-89
AACTT 533225 0.30202213 10.5987015 3
GACTT 633980 0.30058414 6.735645 1
CGCTT 722025 0.29959372 5.7393913 1
GCGAG 1047720 0.29952356 5.1421824 240-247
ATTGT 505920 0.2954588 5.281807 240-247
GAGGT 816555 0.28753832 6.5307336 7
CCCTT 655190 0.28423375 5.717751 3
TTCCT 529455 0.28291893 6.193487 4
TAACT 483450 0.27382925 7.4379325 2
TGTAA 505135 0.2736579 7.901244 25-29
AATCT 471035 0.2667973 7.0132875 2
GTCTT 514445 0.26293242 11.094549 1
TTGGC 661300 0.2624528 7.279195 1
CGGGG 1071385 0.25638554 9.41921 3
CCTCG 727050 0.24491774 5.604668 5
TGCTT 449515 0.22974674 11.353576 1
TGCCT 488240 0.20258808 5.137311 4
TCGCA 516300 0.19873211 7.5118923 7
TAGGC 436240 0.1606067 6.583273 1
TTGCT 262705 0.1342683 5.6038384 4
Produced by FastQC (version 0.10.1)